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Seed development and maturation in African marigold (Tagetes erecta L.) 非洲万寿菊种子的发育与成熟
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.15
C. Murali, S. Jain, M. Joshi, A. Pandey
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引用次数: 1
Diversity and economic importance of viral diseases of pepper (Capsicum spp.) in Eastern Africa 东非辣椒(Capsicum spp.)病毒病的多样性及其经济意义
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.13
B. Waweru, D. Kilalo, D. Miano, J. Kimenju, P. Rukundo
Pepper (Capsicum spp.) is an important vegetable crop in the Eastern Africa region where it is grown mainly by small-scale farmers. However, productivity of the crop is limited by many abiotic and biotic constraints. The abiotic constraints include high cost of seeds and inputs, shortage of improved varieties, drought, low soil fertility and lack of technical knowhow among others, while biotic constraints are dominated by diseases and insect pests. Numerous diseases especially those that are caused by viruses pose serious threats in pepper production. Currently, over 68 viruses are known to affect pepper on a worldwide scale and have serious impact on the quantity and quality of pepper produced. Out of the 68 viruses, 12 belonging to seven genera namely Potyvirus, Cucumovirus, Tobamovirus, Polerovirus, Tospovirus, Alfamovirus and Potexvirus have been reported in Eastern Africa. Pepper veinal mottle virus, Potato virus Y and Cucumber mosaic virus are the most widespread and damaging viruses in the region. Yield losses ranging from 10 to 100% in pepper production have been reported for various viruses namely; Pepper veinal mottle virus, Potato virus Y, Chilli veinal mottle virus, Ethiopian pepper mottle virus, Cucumber mosaic virus, Pepper mild mottle virus, Tobacco mosaic virus, Pepper vein yellows virus, Tomato spotted wilt virus, Alfaalfa mosaic virus and Potato virus X. This article provides an overview of the important pepper viruses occurring in Eastern Africa with reference to their geographic distribution, yield-losses, symptoms, transimission, management methods and research gaps that need to be addressed. It can be concluded that viruses are a major constraint to pepper production in the target region.
在东非地区,胡椒是一种重要的蔬菜作物,主要由小农种植。然而,作物的生产力受到许多非生物和生物限制。非生物限制包括种子和投入成本高、缺乏改良品种、干旱、土壤肥力低和缺乏技术知识等,而生物限制主要是病虫害。许多病害,特别是由病毒引起的病害,对辣椒生产构成严重威胁。目前,在世界范围内已知的影响辣椒的病毒超过68种,严重影响辣椒生产的数量和质量。在这68种病毒中,有12种属于7属,即Potyvirus、Cucumovirus、Tobamovirus、Polerovirus、Tospovirus、alfamvirus和Potexvirus已在东非报告。辣椒脉斑驳病毒、马铃薯Y型病毒和黄瓜花叶病毒是该地区传播最广、危害最大的病毒。据报道,辣椒生产中各种病毒的产量损失从10%到100%不等,即;辣椒纹路斑驳病毒、马铃薯Y型病毒、辣椒纹路斑驳病毒、埃塞俄比亚辣椒斑驳病毒、黄瓜花叶病毒、辣椒轻度斑驳病毒、烟草花叶病毒、辣椒黄脉病毒、番茄斑驳病毒、苜蓿花叶病毒和马铃薯x型病毒。本文综述了发生在东非的重要辣椒病毒的地理分布、产量损失、症状、传播、需要解决的管理方法和研究差距。可以得出结论,病毒是目标地区辣椒生产的主要制约因素。
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引用次数: 5
Modelling individual leaf area of cress and radish using leaf dimensions and weight 利用叶片尺寸和重量模拟水芹和萝卜的个体叶面积
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.04
M. H. Aminifard, H. Bayat, Mehdi Khayyat
Leaf area information is required in various horticultural and physiological studies. Leaf area measurements require easy, quick and possibly non-destructive methods. The objective of this study was to establish equations to estimate leaf area (LA) using length (L), width (W), fresh weight (FW) and dry weight (DW), length×width (L×W), length + width (L+W), width/length (W/L), length2 (L2) and width2 (W2) of cress (Lepidium sativum L.) leaves as a leafy vegetable and radish (Raphanus sativus L.) as a root vegetable. A soilcultured experiment was carried out in 2010 under greenhouse conditions to study relationship between leaf dimension and weight with LA of these two vegetable plants. Observed LA was obtained by an automatic measuring device and leaf dimensions were measured by a ruler. Regression analyses of LA versus L, W, FW, DW, L×W, L+W, W/L, L2 and W2 led several models that could be used for estimating the area of individual cress and radish leaves. A linear model employing FW as an independent variables [LA=0.295 (Fresh W.)+ 1.430] resulted in the most accurate estimate (R2 = 0.912, RMSE = 1.52) of cress LA. For radish, a linear model using W as an independent variable [LA=22.50 (W) + 7.46] showed the most accuracy (R2 = 0.874, RMSE = 11.26) estimating LA. Validation of the regression models showed that the correlation between measured and simulated values using these equations were quite acceptable (R2 = 0.922, 0.876).
在各种园艺和生理学研究中都需要叶面积信息。叶面积测量需要简单、快速且可能无损的方法。本研究的目的是建立以水芹(Lepidium sativum L.)叶为叶菜和萝卜(Raphanus sativus L.)为根菜的长度(L)、宽度(W)、鲜重(FW)和干重(DW)、长度×宽度(L×W)、长度+宽度(L+W)、宽度/长度(W/L)、长度2(L2)和宽度2(W2)估算叶面积(LA)的方程。2010年在温室条件下进行了一项土培试验,研究了这两种蔬菜的叶片尺寸和重量与LA的关系。通过自动测量装置获得观察到的LA,并通过尺子测量叶片尺寸。LA与L、W、FW、DW、L×W、L+W、W/L、L2和W2的回归分析得出了几个可用于估计单个水芹和萝卜叶面积的模型。以FW为自变量[LA=0.295(Fresh W.)+1.430]的线性模型对水芹LA的估计最准确(R2=0.912,RMSE=1.52)。对于萝卜,以W为自变量[LA=22.50(W)+7.46]的线性模型估计LA的准确度最高(R2=0.874,RMSE=12.26)。回归模型的验证表明,使用这些方程的测量值和模拟值之间的相关性是可以接受的(R2=0.922,0.876)。
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引用次数: 0
Successful community-based management of banana wilt caused by Fusarium oxysporum f.sp. cubense Tropical race-4 through ICAR-FUSICONT 香蕉枯萎病的成功社区治理。通过ICAR-FUSICONT
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.06
T. Damodaran, S. Gopal, A. Yadav, P. K. Shukla, M. Muthukumar, N. Kumari, I. Ahmad, Sambhunath Jha, N. Deepak
The profitability of commercial G-9 banana cultivation in the banana growing countries is not only significantly reduced due to serious outbreak of wilt disease caused by Fusarium oxysporum f.sp. cubense Tropical race 4 (Foc TR-4) rather its cultivation is totally stopped at many places. The virulent and polycyclic nature of the pathogen limits the field level success of the control initiatives through chemicals, resistance breeding and biological control. In India, outbreak of Foc TR-4 during 2017 in the districts of Ayodhya in Uttar Pradesh and Katihar in Bihar attracted intensive research program for the management of the disease to avoid nationwide threat. “ICAR-FUSICONT” a bio-formulation comprising of consortia of effective antagonistic and growth promoting microbes on a patent protected modified CSR-BIO media was developed and validated through participatory community based management in the hotspots of Foc TR-4. The current study aims to assess the efficacy of the bio-formulation in the control of the disease, impact on restoring the yield and increasing the income of the banana growers at hotspot sites. The investigation was carried out by descriptive survey from 2017 to 2018 with both adopters and non-adopters of the technology. Results showed that formation of community based organization and community enabled adoption of ICAR-FUSICONT bio-formulation application during the critical growth phases of the crop resulted in an overall decrease in disease incidence in both the states of Uttar Pradesh (U.P.) and Bihar with the adopters. Adopters recorded an average of 6.08 % disease incidence while nonadopters had 45.68 % disease incidence at harvest. An average yield of 25.98 kg/plant with a significantly higher net income of Rs.182949.40/acre was obtained by adopters compared to non-adopters yield of 11.12 kg and an income of Rs. 39917.04. The study also showed that most of the adopters were from the age group of 30 to 40 years with educational status of above secondary level. The results of this study furthermore indicate that the community enabled technology adoption of ICAR-FUSICONT would be a critical component in the control of the banana Fusarium wilt (Foc TR-4) in the areas of disease epidemic.
在香蕉种植国,G-9香蕉商业种植的盈利能力不仅由于尖孢镰刀菌(Fusarium oxysporum f.sp.cubense Tropical race 4,Foc TR-4)引起的枯萎病的严重爆发而显著下降,而且在许多地方完全停止了其种植。病原体的毒性和多环性限制了通过化学品、抗性育种和生物控制的控制举措在实地取得成功。在印度,2017年在北方邦的阿约提亚和比哈尔邦的卡蒂哈尔地区爆发的Foc TR-4引起了对该疾病管理的深入研究,以避免全国范围的威胁。“ICAR-FUSICONT”是一种生物制剂,由专利保护的改良CSR-bio培养基上的有效拮抗和生长促进微生物群落组成,通过在Foc TR-4热点地区的参与式社区管理进行开发和验证。目前的研究旨在评估生物制剂在控制疾病方面的功效,对恢复产量和增加热点地区香蕉种植者收入的影响。该调查于2017年至2018年通过描述性调查对该技术的采用者和非采用者进行。结果表明,在作物的关键生长阶段,基于社区的组织和社区的ICAR-FUSICONT生物制剂应用的形成,导致北方邦和比哈尔邦的发病率总体下降。收养者的发病率平均为6.08%,而未收养者在收获时的发病率为45.68%。采用者的平均产量为25.98公斤/株,净收入为182949.40卢比/英亩,而非采用者的产量为11.12公斤,收入为39991.04卢比。研究还表明,大多数收养者年龄在30至40岁之间,具有中等以上教育水平。本研究的结果进一步表明,社区支持的ICAR-FUSICONT技术的采用将是在疾病流行地区控制香蕉枯萎病(Foc TR-4)的关键组成部分。
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引用次数: 6
Effect of nitrogen fertilization on cucumber downy mildew 施氮对黄瓜霜霉病的影响
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.05
A. Papadaki, G. Markakis, K. Ladomenou, D. Goumas, N. Magan
The objective of this study was to examine the effect of different concentrations of nitrogen on downy mildew severity on cucumber plants. Nitrogen (100-600 mg L-1) was applied to plants grown in pots under greenhouse conditions through irrigation water. The cucumber leaves were inoculated with a zoospore suspension of Pseudoperonospora cubensis, the causal agent of powdery mildew. Disease symptoms in plants treated with 300 mg L-1 nitrogen were significantly lower and 24% severity reduction was recorded. A positive effect on the leaf area was also noticed in cucumber plants with the application of 300 mg L-1 nitrogen. The results indicated that a cubic regression curve can be fitted to the disease progress for downy mildew, regardless of N treatments. Furthermore, the N enrichment affected the NH4-N and NO3-N content in leaves and soil as well as P content in the leaves. The application of 100 mg L -1 N significantly reduced Mn content in leaves. Therefore, nitrogen supply in the form of ammonium nitrate fertilizer could be considered as an efficient method for the control of downy mildew of cucumber.
本研究的目的是研究不同氮浓度对黄瓜霜霉病严重程度的影响。在温室条件下,通过灌溉水对盆栽植株施氮100 ~ 600 mg L-1。用引起白粉病的黄瓜假operonospora cubensis虫孢子悬浮液接种黄瓜叶片。300mg L-1氮素处理的植株病害症状显著降低,严重程度降低24%。施用300 mg L-1氮肥对黄瓜叶片面积也有积极影响。结果表明,无论施氮多少,霜霉病的发病过程均符合三次回归曲线。此外,氮的富集还影响了叶片和土壤中NH4-N和NO3-N含量以及叶片中P含量。施用100 mg L -1 N显著降低了叶片中Mn含量。因此,以硝酸铵肥的形式供氮可被认为是防治黄瓜霜霉病的有效方法。
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引用次数: 3
Influence of Pseudomonas fluorescens and ferrous sulphate on biochemical parameters of peach leaves 荧光假单胞菌和硫酸亚铁对桃叶片生化参数的影响
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.14
S. Singh, Pushpa Singh, P. Shrivastava, D. C. Dimri, S. Tripathi, A. Narayan
Foliar fertilization and microbe supplemented iron bioavailability can fulfill the iron demand of plants. Plants of low-chill peach cv. Pant Peach-1 were selected to find out the effect of Pseudomonas flouresecens PBAP-27 inoculation along with foliar spray of ferrous sulphate. The experiment consisted of eight treatments viz. T1: control (water spray), T2: P. fluorescens PBAP-27, T3: FeSO4. 7H2O (0.5 %) spray once, T4: FeSO4. 7H2O (0.5 %) spray twice, T5: FeSO4. 7H2O (0.5 %) spray thrice, T6: P. fluorescens PBAP-27+FeSO4.7H2O (0.5 %) spray once, T7: P. fluorescens PBAP-27+FeSO4.7H2O (0.5 %) spray twice, T8: P. fluorescens PBAP-27+FeSO4.7H2O (0.5 %) spray thrice. Leaf area, chlorophyll content, active iron, total iron, ferric iron and iron: manganese ratios of leaves were studied. Maximum leaf area was recorded with P. fluorescens PBAP-27+FeSO4.7H2O (0.5 %) by spraying twice. Whereas, chlorophyll content, active iron, total iron, ferric iron and iron: manganese ratio were recorded higher in P. fluorescens PBAP-27+FeSO4.7H2O (0.5 %) sprayed thrice. Findings indicate that the three foliar spray of ferrous sulphate (0.5 %) along with inoculation of P. fluorescens strain can be used as useful strategy for iron fertilization for improving physiological parameters (leaf area and chlorophyll content) and leaves iron status (active iron, total iron, ferric iron ) in low chill peach.
叶面施肥和微生物补铁的生物利用度可以满足植物对铁的需求。选用低冷桃Pant peach-1植株,研究了荧光假单胞菌PBAP-27接种和硫酸亚铁叶面喷雾的效果。实验由八个处理组成,即T1:对照(喷水),T2:荧光假单胞菌PBAP-27,T3:FeSO4。7H2O(0.5%)喷洒一次,T4:FeSO4。7H2O(0.5%)喷雾两次,T5:FeSO4。7H2O(0.5%)喷三次,T6:荧光假单胞菌PBAP-27+FeSO4.7H2O(0.5%。对叶片面积、叶绿素含量、活性铁、全铁、三价铁和铁锰比进行了研究。荧光假单胞菌PBAP-27+FeSO4.7H2O(0.5%)通过喷洒两次记录最大叶面积。而荧光假单胞菌PBAP-27+FeSO4.7H2O(0.5%)三次喷雾的叶绿素含量、活性铁、总铁、三价铁和铁锰比均较高。结果表明,三次叶面喷施0.5%硫酸亚铁和接种荧光假单胞菌可作为改善低冷桃生理参数(叶面积和叶绿素含量)和叶片铁状况(活性铁、总铁、三价铁)的有效施肥策略。
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引用次数: 0
King chilli (Capsicum chinense Jacq.), The Indias hottest chilli- An Overview 王辣椒(Capsicum chinense Jacq.),印度最辣的辣椒-综述
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.09
S. Malakar, S. Sarkar, Nitin Kumar
King chilli (Capsicum chinense Jacq.) is an indigenous variety of capsicum to the northeast region of India and has been familiar as the hottest chilli in the World. In this article possible nutritional value of king chilli was studied for the health benefit. It has also been great prospective for its pungency and medicinal value utilized by the native people of the northeast India. It is consumed in different form as normal chilli but have especial demand on national and international markets for its extra powerful pungency and aroma. Even though being a valuable crop with high potential value for income sources for the indigenous people, so it is essential to emphasis on studies directed towards the different systematic production (morphological, cultivation practices, postharvest processing and marketing) of king chilli. Due to high demand there need to standardize the production and postharvest technology which may help to improve the yield, quality and shelf life extension for long chain marketing and distribution. The present review is focused on production and processing practices of king chilli.
国王辣椒(Capsicum chinense Jacq.)是印度东北部地区的本土辣椒品种,是世界上最辣的辣椒。本文对王辣椒的营养价值进行了初步探讨,以期对人体健康有益。它的辛辣和药用价值也被印度东北部的土著居民所利用,具有很大的前景。它的消费形式与普通辣椒不同,但由于其特别强烈的辛辣和香气,在国内和国际市场上有着特别的需求。尽管王辣椒是一种有价值的作物,对土著人民的收入来源具有很高的潜在价值,所以必须强调对王辣椒不同系统生产(形态、栽培方法、采后加工和销售)的研究。由于需求量很大,因此需要标准化生产和采后技术,这可能有助于提高产量、质量和延长长链营销和分销的保质期。本文就王辣椒的生产和加工实践作一综述。
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引用次数: 1
Effect of crezacin and humic acid on growth and physiological aspects of tomato plants (Lycopersicon esculentum) 百忧解和腐殖酸对番茄生长和生理特性的影响
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.11
M. Abdelkader, M. Puchkov, M. Lysakov, E. Loktionova, A. A. Suliman
This study aims to enhance growth characteristics and quality parameters for tomato plants (Lycopersicon esculentum) by applying different doses of Crezacin (synthetic) and Energene (natural) as growth regulators instead of Chemical fertilization. A specific experiment has been carried out to study the effect of two types of plant growth regulators Crezacin: Active substances (475 g/L): Orth-o-cresoxyacetic acid triethanolammonium salt with applied doses (100, 200 and 300 ppm) and Energene: active substances (700 g/kg) humic acid with applied doses (250, 500 and 700 ppm) on growth and physiological characteristics of tomato plants (Kendras F1). Both Crezacin and Energene were applied three times (Soaking tomato seeds for 24 hours before sowing, spraying on plants at 35 DAP, spraying on plants at 70 DAP). Application of humic acid “Ener-700” increased plant height (78.0 cm), number of leaves/ plant (57.8), fresh weight (250.2 g), dry weight (75.1 g) of arial parts, flowers number (48.1), fruits number (35.1) and fruit weight (64.0 g). Also using “ Ener-700” improved tomato fruit’s quality by enhancing level of dry matter (7.6 %), sugar contents (5.8 oBrix), Ascorbic acid (26.9 mg 100 g-1), Maturity Index (12.61) and Taste Index (1.09), while level of nitrate rose by increasing humic acid and the maximum level (22.0 mg kg-1) obtained from Ener-700. Applying Ener-500 gave the maximum titratable acidity (0.48%) and carotenoids contents (4.89 mg 100 g-1)
本研究旨在通过施用不同剂量的克忧锌(合成)和Energene(天然)作为生长调节剂,而不是化学施肥,来提高番茄(Lycopersicon esculentum)的生长特性和质量参数。本试验研究了两种植物生长调节剂克扎辛:活性物质(475 g/L):施用剂量(100、200和300 ppm)的邻甲氧基乙酸三乙醇胺盐和施用剂量(250、500和700 ppm)的Energene:活性物质(700 g/kg)腐殖酸对番茄生长和生理特性的影响(肯德拉斯F1)。Crezacin和Energene都施用了三次(播种前将番茄种子浸泡24小时,以35DAP喷洒在植物上,以70DAP喷洒到植物上)。施用腐殖酸“Ener-700”可增加株高(78.0cm)、单株叶数(57.8)、鲜重(250.2g)、干重(75.1g)、花数(48.1)、果数(35.1)和果重(64.0g)。使用Ener-700可提高番茄果实的干物质(7.6%)、含糖量(5.8oBrix)、抗坏血酸(26.9mg/100g-1)、成熟度指数(12.61)和味觉指数(1.09),从而改善番茄果实的品质。应用Ener-500可获得最大可滴定酸度(0.48%)和类胡萝卜素含量(4.89 mg 100 g-1)
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引用次数: 1
Fruit characterization of jackfruit (Artocarpus heterophyllus Lam.) local genotypes under coffee ecosystem of lower pulney hills in Tamil Nadu, India. 印度泰米尔纳德邦下普尔尼丘陵咖啡生态系统下菠萝蜜(Artocarpus heterophyllus Lam.)本地基因型果实特征
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.08
K. Chandrashekar, R. Vijayakumar, S. Subramanian, M. Kavino, A. J. Joel
n experiment was conducted to evaluation of jackfruit (Artocarpus heterophyllus Lam.,) local genotypes under coffee ecosystem of lower pulney hills at Horticultural Research Station, Thadiyankudisai and its adjoining areas, Tamil Nadu Agricultural University, Coimbatore during year 2016-2017 with an objective to characterize the promising local genotypes for commercial cultivation and studies of genetic improvement with jackfruit, five fruits of each of thirty five genotypes were evaluated based on IPGRI, 2000 (Bioversity international) jackfruit descriptor. Fruit characters viz., bearing habit, bearing position, clustering habit, shape, length, diameter, weight, stalk attachment, spine density per 25 cm2, fruit rind weight and flake characters viz., total weight of flakes per fruit, weight of individual flake with seed, weight of individual flake without seed, number of flakes per fruit, flakes: fruit ratio, rind: flakes ratio, flake length, flake width, shape of flake, flake colour. Results were evaluated by descriptive statistics viz., mean, standard deviation, coefficient of variation, standard error of mean. It was concluded that a more variation of fruit character was found under lower pulney hills of Tamil Nadu, it will help to breeder for accounting potential fruit character in future.
2016-2017年,在哥印拜陀泰米尔纳德邦农业大学Thadiyankudisai及其邻近地区园艺研究所,对下普尔尼丘陵咖啡生态系统下的菠萝蜜(Artocarpus heterophyllus Lam.,)进行了本地基因型评价试验,目的是表征具有商业种植前景的本地基因型和菠萝蜜遗传改良研究。根据IPGRI, 2000 (Bioversity international)菠萝蜜描述符对35个基因型各5个果实进行了评价。果实性状:结实习性、结实位置、聚类习性、形状、长度、直径、重量、柄附着、每25cm2棘密度、果皮重量和叶片性状:单果叶片总重量、带籽单片重量、无籽单片重量、单果叶片数、叶片:果比、果皮:叶片比、叶片长度、叶片宽度、叶片形状、叶片颜色。结果用描述性统计进行评价,即平均值、标准差、变异系数、平均值的标准误差。结果表明,在泰米尔纳德邦低普尔尼丘陵地区发现了更多的果实性状变异,这将有助于今后育种人员对潜在果实性状的核算。
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引用次数: 0
Elimination of Potyvirus and Carlavirus from Infected shallot bulbs 从受感染的小葱球茎中消除Potyvirus和Carlavirus
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.07
P. H. Putri, S. Hidayat, D. Dinarti
Viral diseases on shallot in Indonesia has been reported and have important role in yield loss. Viral diseases are easily transmitted via infected bulbs in generations and in regions. Cultivation of virus-free bulbs is considered the most effective method of controlling viral diseases, so the production of virus-free bulb are important and necessary. Elimination of viruses on local cultivars was achieved using shoot tip culture combined with thermotherapy. In this research, the method for elimination of Potyvirus and Carlavirus from shallot bulbs was developed using combination of thermotherapy (30 °C, 37 °C, in homogenous and heterogenous condition) with different size of explant shoot tip culture (1 mm, 2 mm, 3 mm). The result showed that virus-free plantlets could be derived from 1 mm-explant. Treatment combination of 2 mm-explant with homogenous temperture 37 °C was also able to eliminate virus.
印度尼西亚的葱病毒性病害已被报道,并在产量损失中起重要作用。病毒性疾病很容易通过受感染的球茎在世代和地区间传播。无病毒球茎的栽培被认为是控制病毒病害最有效的方法,因此无病毒球茎的生产是重要和必要的。采用茎尖培养与热疗相结合的方法,实现了对地方品种的病毒脱除。本研究采用热疗(30°C、37°C、同质和异质条件)和不同外植茎尖培养大小(1 mm、2 mm、3 mm)相结合的方法,对大葱鳞茎中Potyvirus和carlavvirus进行了脱除。结果表明,1 mm外植体可获得脱毒苗。2 mm外植体与均匀温度37℃联合处理也能杀灭病毒。
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引用次数: 2
期刊
Journal of Applied Horticulture
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