Propolis is a resinous substance that bees use todefend their colonies against any pathogen. It has beenused in traditional medicine since ancient times because ofits therapeutic properties. Pollen analysis of propolis of AinTrid has shown that the region of Ain Trid is rich in plantspecies visited by bees (31 genus identified) and that themajority of these species are considered as medicinal plants,which gives propolis an important place in wound healing.The study of the wound healing activity is carried out in vivoon theWistar rats divided into 3 groups: group 1 treated withpropolis ointment, group 2 treated with commercial cream,and group 3 considered as untreated. The duration of treatmentwas estimated to be 23 days. The results obtainedshowed that the group treated by propolis ointment showeda high cure rate, in which the percentage of wound closurereached up to 94.93% ± 5.29 in the group 1, while in thegroup 2 arrived to 79.97% ± 13.01, and in group 3 was72.55% ± 19.94. The results demonstrated also that the numberof days required to contract 50% of wound area wasdifferent among the groups, in the 12th day in group 1,16th day in group 2, and 18th day post wounding in group 3.These results have shown that propolis can be prepared andused in traditional medicine as a natural ointment for thetreatment of skin diseases in our region.
{"title":"Wound Healing Activity of Propolis of West Algeria","authors":"M. Debab, F. Toumi-Benali, H. Salem","doi":"10.3166/PHYTO-2018-0075","DOIUrl":"https://doi.org/10.3166/PHYTO-2018-0075","url":null,"abstract":"Propolis is a resinous substance that bees use todefend their colonies against any pathogen. It has beenused in traditional medicine since ancient times because ofits therapeutic properties. Pollen analysis of propolis of AinTrid has shown that the region of Ain Trid is rich in plantspecies visited by bees (31 genus identified) and that themajority of these species are considered as medicinal plants,which gives propolis an important place in wound healing.The study of the wound healing activity is carried out in vivoon theWistar rats divided into 3 groups: group 1 treated withpropolis ointment, group 2 treated with commercial cream,and group 3 considered as untreated. The duration of treatmentwas estimated to be 23 days. The results obtainedshowed that the group treated by propolis ointment showeda high cure rate, in which the percentage of wound closurereached up to 94.93% ± 5.29 in the group 1, while in thegroup 2 arrived to 79.97% ± 13.01, and in group 3 was72.55% ± 19.94. The results demonstrated also that the numberof days required to contract 50% of wound area wasdifferent among the groups, in the 12th day in group 1,16th day in group 2, and 18th day post wounding in group 3.These results have shown that propolis can be prepared andused in traditional medicine as a natural ointment for thetreatment of skin diseases in our region.","PeriodicalId":39888,"journal":{"name":"Phytotherapie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42671263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Djoko, L. T. Nague, R. Chougouo, D. Wouessidjewe
Le beurre de karité et le beurre de cacao présentent de grandes similitudes d’utilisation en cosmétique et en agroalimentaire. Nous avons voulu, dans la présente étude, rechercher les voies et moyens de l’utilisation du beurre de karité dans le secteur pharmaceutique pour la fabrication des suppositoires et des ovules. Des échantillons de beurre de karité produits dans la région nord du Cameroun ont été achetés sur le marché de Bafoussam (région ouest). Après l’analyse physique et chimique des échantillons, nous avons procédé à la recherche d’un améliorant qui, incorporé au beurre de karité, pourrait permettre d’obtenir un excipient compatible avec la fabrication des suppositoires et des ovules. Nous avons ainsi essayé successivement comme améliorant la cire blanche et la silice colloïdale aux taux respectifs de 1, 2, 4 et 6 %. Pour vérifier la pertinence de notre choix, nous avons fabriqué et soumis au contrôle deux médicaments témoins en utilisant d’une part le beurre de karité brut local et d’autre part le beurre de karité raffiné importé de France (laboratoire Aroma). À température ambiante, le beurre de karité brut était collant et huileux au toucher, avec une consistance pâteuse. Son point de fusion était de 34 °C. L’indice d’acide était de 1,56, l’indice d’ester de 170,98, l’indice de saponification de 181,84, l’indice de peroxyde de 2,90 et la densité de 0,90. Pour ce qui est de la recherche de l’améliorant, le meilleur résultat a été obtenu avec la silice colloïdale anhydre (AEROSIL® 200) à 4 %. Le nouvel excipient obtenu avait une consistance convenable et un point de fusion favorable (36 °C) à l’utilisation comme excipient pour suppositoires. Tous les médicaments témoins (suppositoires enfants de paracétamol dosés à 300 mg et ovules de métronidazole dosés à 500 mg) fabriqués aussi bien avec le beurre de karité brut local que le beurre de karité raffiné importé ont répondu favorablement aux tests pharmacotechniques prévus par la pharmacopée européenne. Ce résultat des tests pharmacotechniques nous permet de conclure que le beurre de karité renforcé par 4 % de silice colloïdale anhydre peut être valablement utilisé comme excipient pour suppositoires et ovules.
{"title":"Essai de formulation de suppositoires et ovules à base de beurre de karité comme excipient","authors":"E. Djoko, L. T. Nague, R. Chougouo, D. Wouessidjewe","doi":"10.3166/PHYTO-2018-0065","DOIUrl":"https://doi.org/10.3166/PHYTO-2018-0065","url":null,"abstract":"Le beurre de karité et le beurre de cacao présentent de grandes similitudes d’utilisation en cosmétique et en agroalimentaire. Nous avons voulu, dans la présente étude, rechercher les voies et moyens de l’utilisation du beurre de karité dans le secteur pharmaceutique pour la fabrication des suppositoires et des ovules. Des échantillons de beurre de karité produits dans la région nord du Cameroun ont été achetés sur le marché de Bafoussam (région ouest). Après l’analyse physique et chimique des échantillons, nous avons procédé à la recherche d’un améliorant qui, incorporé au beurre de karité, pourrait permettre d’obtenir un excipient compatible avec la fabrication des suppositoires et des ovules. Nous avons ainsi essayé successivement comme améliorant la cire blanche et la silice colloïdale aux taux respectifs de 1, 2, 4 et 6 %. Pour vérifier la pertinence de notre choix, nous avons fabriqué et soumis au contrôle deux médicaments témoins en utilisant d’une part le beurre de karité brut local et d’autre part le beurre de karité raffiné importé de France (laboratoire Aroma). À température ambiante, le beurre de karité brut était collant et huileux au toucher, avec une consistance pâteuse. Son point de fusion était de 34 °C. L’indice d’acide était de 1,56, l’indice d’ester de 170,98, l’indice de saponification de 181,84, l’indice de peroxyde de 2,90 et la densité de 0,90. Pour ce qui est de la recherche de l’améliorant, le meilleur résultat a été obtenu avec la silice colloïdale anhydre (AEROSIL® 200) à 4 %. Le nouvel excipient obtenu avait une consistance convenable et un point de fusion favorable (36 °C) à l’utilisation comme excipient pour suppositoires. Tous les médicaments témoins (suppositoires enfants de paracétamol dosés à 300 mg et ovules de métronidazole dosés à 500 mg) fabriqués aussi bien avec le beurre de karité brut local que le beurre de karité raffiné importé ont répondu favorablement aux tests pharmacotechniques prévus par la pharmacopée européenne. Ce résultat des tests pharmacotechniques nous permet de conclure que le beurre de karité renforcé par 4 % de silice colloïdale anhydre peut être valablement utilisé comme excipient pour suppositoires et ovules.","PeriodicalId":39888,"journal":{"name":"Phytotherapie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44299275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Methanol extract from Lippia graveolens (Mexican oregano) was studied in order to identify inhibitory bioactives for protein tyrosine phosphatase 1B (PTP1B). Known flavone as lutein (1), and another flavone glycoside such as lutein-7-o-glucoside (2), 6-hydroxy-lutein-7-ohexoside (3) and lutein-7-o-ramnoide (4) were isolated from methanol extract of aerial parts of the Lippia graveolens. All isolates were identified based on extensive spectroscopic data analysis, including UV, IR, NMR, MS and compared with spectroscopic data previously reported. These flavones were evaluated for PTP1B inhibitory activity. Among them, compounds 1 and 3 displayed potential inhibitory activity against PTP1B with IC50 values of 7.01 ± 1.25 μg/ml and 18.4 μg/ml, respectively. In addition, compound 2 and 4 showed moderate inhibitory activity with an IC50 value of 23.8 ± 6.21 and 67.8 ± 5.80 μg/ml respectively. Among the four compounds, luteolin was found to be the most potent PTP1B inhibitor compared to the positive control ursolic acid, with an IC50 value of 8.12 ± 1.06 μg/ml. These results indicate that flavonoids constituents contained in Lippia graveolens can be considered as a natural source for the treatment of type 2 diabetes.
{"title":"Inhibition of Protein Tyrosine Phosphatase 1B by Lutein Derivatives isolated from Mexican Oregano (Lippia graveolens)","authors":"R. Perez-Gutierrez","doi":"10.3166/PHYTO-2018-0074","DOIUrl":"https://doi.org/10.3166/PHYTO-2018-0074","url":null,"abstract":"Methanol extract from Lippia graveolens (Mexican oregano) was studied in order to identify inhibitory bioactives for protein tyrosine phosphatase 1B (PTP1B). Known flavone as lutein (1), and another flavone glycoside such as lutein-7-o-glucoside (2), 6-hydroxy-lutein-7-ohexoside (3) and lutein-7-o-ramnoide (4) were isolated from methanol extract of aerial parts of the Lippia graveolens. All isolates were identified based on extensive spectroscopic data analysis, including UV, IR, NMR, MS and compared with spectroscopic data previously reported. These flavones were evaluated for PTP1B inhibitory activity. Among them, compounds 1 and 3 displayed potential inhibitory activity against PTP1B with IC50 values of 7.01 ± 1.25 μg/ml and 18.4 μg/ml, respectively. In addition, compound 2 and 4 showed moderate inhibitory activity with an IC50 value of 23.8 ± 6.21 and 67.8 ± 5.80 μg/ml respectively. Among the four compounds, luteolin was found to be the most potent PTP1B inhibitor compared to the positive control ursolic acid, with an IC50 value of 8.12 ± 1.06 μg/ml. These results indicate that flavonoids constituents contained in Lippia graveolens can be considered as a natural source for the treatment of type 2 diabetes.","PeriodicalId":39888,"journal":{"name":"Phytotherapie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42312416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Boukhira, F. Bousta, S. Moularat, A. Abdellaoui, Z. B. Ouaritini, D. Bousta
The aim of this study is to evaluate the preservative efficacy of Origanum elongatum essential oil in topical cream under a challenge test that was performed following the standard procedure proposed by the European Pharmacopoeia, using standardized microorganisms. Essential oil was analyzed by GC–MS (gas chromatography–mass spectrometry). The antimicrobial activity of this essential oil was investigated by agar diffusion methods and broth microdilution assay to determine the minimum inhibitory concentration of essential oil required for preservation. The results demonstrated that the preservation effect was effective at 2% (v/w) of Origanum elongatum essential oil in topical cream, satisfying criterion A against Staphylococcus aureus, Escherichia coli and Aspergillus brasiliensis. However, the essential oil was ineffective against Pseudomonas aeruginosa with a reduction of 102 within 7 days of challenge and no increase up to the 28th day and against Candida albicans in the challenge test. Carvacrol predominated (63.06%), followed by the two monoterpenes hydrocarbons: γ-terpinene (15.99%) and p-cymene (9.51%). Our study highlighted the preservative power of Origanum elongatum essential oil as an alternative solution to chemical preservatives.
{"title":"Evaluation of the Preservative Properties of Origanum elongatum Essential Oil in a Topically Applied Formulation Under a Challenge Test","authors":"S. Boukhira, F. Bousta, S. Moularat, A. Abdellaoui, Z. B. Ouaritini, D. Bousta","doi":"10.3166/PHYTO-2018-0067","DOIUrl":"https://doi.org/10.3166/PHYTO-2018-0067","url":null,"abstract":"The aim of this study is to evaluate the preservative efficacy of Origanum elongatum essential oil in topical cream under a challenge test that was performed following the standard procedure proposed by the European Pharmacopoeia, using standardized microorganisms. Essential oil was analyzed by GC–MS (gas chromatography–mass spectrometry). The antimicrobial activity of this essential oil was investigated by agar diffusion methods and broth microdilution assay to determine the minimum inhibitory concentration of essential oil required for preservation. The results demonstrated that the preservation effect was effective at 2% (v/w) of Origanum elongatum essential oil in topical cream, satisfying criterion A against Staphylococcus aureus, Escherichia coli and Aspergillus brasiliensis. However, the essential oil was ineffective against Pseudomonas aeruginosa with a reduction of 102 within 7 days of challenge and no increase up to the 28th day and against Candida albicans in the challenge test. Carvacrol predominated (63.06%), followed by the two monoterpenes hydrocarbons: γ-terpinene (15.99%) and p-cymene (9.51%). Our study highlighted the preservative power of Origanum elongatum essential oil as an alternative solution to chemical preservatives.","PeriodicalId":39888,"journal":{"name":"Phytotherapie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46868899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bouyahya, Fatima-Ezzahrae Guaouguaou, N. Dakka, Y. Bakri
Le quorum sensing (QS) est une communication bacterienne fondee sur des molecules-signaux ligands-recepteurs. Ces derniers sont des pheromones bacteriennes dites auto-inductrices secretees par les bacteries pour reguler certaines fonctions telles que la resistance aux antibiotiques et la formation de biofilms. Le dechiffrage moleculaire de ce systeme a permis de decortiquer de nouvelles cibles moleculaires contrebalancant ainsi les mecanismes de resistance pour lutter contre les maladies infectieuses. Les substances naturelles des plantes medicinales sont montrees efficaces contre les mediateurs du QS dans les dernieres annees. Elles peuvent inhiber, interferer ou sequestrer les auto-inducteurs du QS. Ainsi, elles peuvent supprimer les phenotypes qui en resultent tels que la bioluminescence et la formation de biofilms. Dans le present travail, nous discutons le systeme du QS et leur implication dans les maladies infectieuses, ainsi nous montrons leur importance comme cible therapeutique specifique de substances naturelles extraites des plantes medicinales.
{"title":"Quorum sensing : une nouvelle cible anti-infectieuse des plantes médicinales","authors":"A. Bouyahya, Fatima-Ezzahrae Guaouguaou, N. Dakka, Y. Bakri","doi":"10.3166/PHYTO-2018-0042","DOIUrl":"https://doi.org/10.3166/PHYTO-2018-0042","url":null,"abstract":"Le quorum sensing (QS) est une communication bacterienne fondee sur des molecules-signaux ligands-recepteurs. Ces derniers sont des pheromones bacteriennes dites auto-inductrices secretees par les bacteries pour reguler certaines fonctions telles que la resistance aux antibiotiques et la formation de biofilms. Le dechiffrage moleculaire de ce systeme a permis de decortiquer de nouvelles cibles moleculaires contrebalancant ainsi les mecanismes de resistance pour lutter contre les maladies infectieuses. Les substances naturelles des plantes medicinales sont montrees efficaces contre les mediateurs du QS dans les dernieres annees. Elles peuvent inhiber, interferer ou sequestrer les auto-inducteurs du QS. Ainsi, elles peuvent supprimer les phenotypes qui en resultent tels que la bioluminescence et la formation de biofilms. Dans le present travail, nous discutons le systeme du QS et leur implication dans les maladies infectieuses, ainsi nous montrons leur importance comme cible therapeutique specifique de substances naturelles extraites des plantes medicinales.","PeriodicalId":39888,"journal":{"name":"Phytotherapie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41616987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sari, S. Nemmiche, Houcine Benmehdi, A. Amrouche, A. Hamadi, D. C. Sari
The purpose of the current study is to explore the prominent role of the fixed oil of Citrullus colocynthis seeds as a natural remedy to obesity. Wistar rats were subjected to different diets; one being an 8 week high-fat diet that was established to induce obesity in rats. The results from our experiment show a significant correction in body weight, blood parameters, and in particular, the total cholesterol, triglycerides, HDL-C (high-density lipoprotein cholesterol), and glycemia. There was also an improvement of the redox status shown by the significant increase in antioxidant vitamins levels and decrease in malondialdehyde contents as well as an increase in the hepatic enzymes activities in Citrullus colocynthis seeds oil treated rats compared to obese rats and olive oil treated rats. The results of this study demonstrated that Citrullus colocynthis seeds oil prompted a corrective effect in the antioxidant defense systems, restored insulin sensitivity, and improved lipid profile. To conclude, our study suggests thatCitrullus colocynthisseeds oil is a promising natural tool to combat obesity and its associated complications.
{"title":"Hypolipidemic and Antioxidant Effects ofCitrullus colocynthisSeeds Oil in High-Fat Diets Induced Obese Rats","authors":"M. Sari, S. Nemmiche, Houcine Benmehdi, A. Amrouche, A. Hamadi, D. C. Sari","doi":"10.3166/PHYTO-2018-0066","DOIUrl":"https://doi.org/10.3166/PHYTO-2018-0066","url":null,"abstract":"The purpose of the current study is to explore the prominent role of the fixed oil of Citrullus colocynthis seeds as a natural remedy to obesity. Wistar rats were subjected to different diets; one being an 8 week high-fat diet that was established to induce obesity in rats. The results from our experiment show a significant correction in body weight, blood parameters, and in particular, the total cholesterol, triglycerides, HDL-C (high-density lipoprotein cholesterol), and glycemia. There was also an improvement of the redox status shown by the significant increase in antioxidant vitamins levels and decrease in malondialdehyde contents as well as an increase in the hepatic enzymes activities in Citrullus colocynthis seeds oil treated rats compared to obese rats and olive oil treated rats. The results of this study demonstrated that Citrullus colocynthis seeds oil prompted a corrective effect in the antioxidant defense systems, restored insulin sensitivity, and improved lipid profile. To conclude, our study suggests thatCitrullus colocynthisseeds oil is a promising natural tool to combat obesity and its associated complications.","PeriodicalId":39888,"journal":{"name":"Phytotherapie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48537953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Guinobert, V. Bardot, L. Berthomier, I. Ripoche, C. Faivre, L. Haddioui, H. Belkhelfa
L’objectif etait de realiser l’empreinte phytochimique par HPTLC et d’identifier par LC/MS les principaux composes d’un extrait hydroethanolique de cones frais de cypres (Cupressus sempervirens L.) puis d’evaluer l’activite virucide de cet extrait sur quatre virus infectant l’Homme et trois virus bovins. L’analyse phytochimique a montre la presence de tanins, de flavonoides, d’acides amines et de glucides. A 40 % (v/v), au contact des virus pendant 60 minutes a 37 °C, l’extrait a eu une activite virucide sur le coronavirus, le virus parainfluenza de type 3, le virus respiratoire syncytial bovin et le rotavirus bovin. A 80 % (v/v), l’extrait a eu une activite virucide sur le virus influenza A-H1N1, le rhinovirus et l’herpesvirus bovin de type 1. Ces resultats invitent a poursuivre les travaux sur cet extrait de cypres et a explorer ses effets antiviraux in vivo.
{"title":"Activité virucide in vitro d’un extrait de cyprès sur des virus humains et bovins","authors":"I. Guinobert, V. Bardot, L. Berthomier, I. Ripoche, C. Faivre, L. Haddioui, H. Belkhelfa","doi":"10.3166/PHYTO-2018-0064","DOIUrl":"https://doi.org/10.3166/PHYTO-2018-0064","url":null,"abstract":"L’objectif etait de realiser l’empreinte phytochimique par HPTLC et d’identifier par LC/MS les principaux composes d’un extrait hydroethanolique de cones frais de cypres (Cupressus sempervirens L.) puis d’evaluer l’activite virucide de cet extrait sur quatre virus infectant l’Homme et trois virus bovins. L’analyse phytochimique a montre la presence de tanins, de flavonoides, d’acides amines et de glucides. A 40 % (v/v), au contact des virus pendant 60 minutes a 37 °C, l’extrait a eu une activite virucide sur le coronavirus, le virus parainfluenza de type 3, le virus respiratoire syncytial bovin et le rotavirus bovin. A 80 % (v/v), l’extrait a eu une activite virucide sur le virus influenza A-H1N1, le rhinovirus et l’herpesvirus bovin de type 1. Ces resultats invitent a poursuivre les travaux sur cet extrait de cypres et a explorer ses effets antiviraux in vivo.","PeriodicalId":39888,"journal":{"name":"Phytotherapie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49265034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：