Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00489
Chengyan Zhang, Xiaoying Cheng, Jianjun Wang, Lu Zhang, Yingyang Du
{"title":"Spatial-temporal Variations of Alkaline Phosphatase Activity in the Kuilei Lake and Their Influencing Factors: Spatial-temporal Variations of Alkaline Phosphatase Activity in the Kuilei Lake and Their Influencing Factors","authors":"Chengyan Zhang, Xiaoying Cheng, Jianjun Wang, Lu Zhang, Yingyang Du","doi":"10.3724/SP.J.1145.2013.00489","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00489","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00286
Luo Ke-ju, Zhu Jianguo, L. Gang, Tang Haoye, Liang Chunhua, Zeng Qing
An ozone free-air control enrichment(O3-FACE) experiment was conducted in 2011 to study the effects of O3 and high nitrogen(N) supply on dry matter production and distribution of rice.Two cultivars,Shanyou 63(SY63,three-line hybrid indica cultivar) and Yangdao 6(YD6,inbred indica cultivar),were investigated.The results showed:(1) Elevated O3 concentration significantly reduced the biomass of stems and foliages in the mid-filling stage by 30.20%,21.95% for SY63 and 21.71%,11.12% for YD6,respectively,due to the significant decrease of the net photosynthetic rate.O3 exposure also significantly reduced the yield,the biomass of roots and panicles in the harvesting stage by 45.75%,48.62%,37.15% for SY63,and 28.91%,35.22%,28.01% for YD6,respectively.Increasing N supply could mitigate O3-induced reduction of the net photosynthetic rate,the dry matter and yield,where the yield and biomass of stems,foliages,roots and panicles were changed by elevated O3only by-26.88%,-13.52%,-5.84%,-26.33%,-16.19% for SY63,and-12.68%,-16.26%,+5.19%,-14.55%,-20.22% for YD6,respectively;(2) In SY63 and YD6 exposed to O3,the root-shoot ratio and root dry matter distribution in whole plant decreased significantly,and the ratio of foliage dry matter distribution increased significantly,but there was no significant effect on the ratio of stem or panicle dry matter distribution.High N supply could increase the ratio of root or foliage dry matter and the root-shoot ratio at O3-FACE treatments;(3) O3 exposure could reduce N accumulation of SY63 and YD6,which would show upward trends after high N treatments.These results implied that increasing N supply could mitigate O3-induced changes in dry matter production and distribution of SY63 and YD6.
{"title":"Responses of Dry Matter Production and Distribution in Rice (Oryza sativa L.) to Ozone and High Nitrogen Supply","authors":"Luo Ke-ju, Zhu Jianguo, L. Gang, Tang Haoye, Liang Chunhua, Zeng Qing","doi":"10.3724/SP.J.1145.2013.00286","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00286","url":null,"abstract":"An ozone free-air control enrichment(O3-FACE) experiment was conducted in 2011 to study the effects of O3 and high nitrogen(N) supply on dry matter production and distribution of rice.Two cultivars,Shanyou 63(SY63,three-line hybrid indica cultivar) and Yangdao 6(YD6,inbred indica cultivar),were investigated.The results showed:(1) Elevated O3 concentration significantly reduced the biomass of stems and foliages in the mid-filling stage by 30.20%,21.95% for SY63 and 21.71%,11.12% for YD6,respectively,due to the significant decrease of the net photosynthetic rate.O3 exposure also significantly reduced the yield,the biomass of roots and panicles in the harvesting stage by 45.75%,48.62%,37.15% for SY63,and 28.91%,35.22%,28.01% for YD6,respectively.Increasing N supply could mitigate O3-induced reduction of the net photosynthetic rate,the dry matter and yield,where the yield and biomass of stems,foliages,roots and panicles were changed by elevated O3only by-26.88%,-13.52%,-5.84%,-26.33%,-16.19% for SY63,and-12.68%,-16.26%,+5.19%,-14.55%,-20.22% for YD6,respectively;(2) In SY63 and YD6 exposed to O3,the root-shoot ratio and root dry matter distribution in whole plant decreased significantly,and the ratio of foliage dry matter distribution increased significantly,but there was no significant effect on the ratio of stem or panicle dry matter distribution.High N supply could increase the ratio of root or foliage dry matter and the root-shoot ratio at O3-FACE treatments;(3) O3 exposure could reduce N accumulation of SY63 and YD6,which would show upward trends after high N treatments.These results implied that increasing N supply could mitigate O3-induced changes in dry matter production and distribution of SY63 and YD6.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00342
Xiaobo Wang, Meirong Li, Xue Li, Jianli Huang, Ying Huang, L. Du, Ribo Huang, Yutuo Wei
{"title":"Gene Cloning, Expression and Enzymatic Characterization of a High Maltose-producing α-amylase from Saccharomonospora viridis : Gene Cloning, Expression and Enzymatic Characterization of a High Maltose-producing α-amylase from Saccharomonospora viridis","authors":"Xiaobo Wang, Meirong Li, Xue Li, Jianli Huang, Ying Huang, L. Du, Ribo Huang, Yutuo Wei","doi":"10.3724/SP.J.1145.2013.00342","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00342","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00375
Yang Shanshan, Zhang Hongrui, Fu Sheng-mi, Li Xinli, C. Jing
Polyhydroxyalkanoate(PHA) is a family of carbon and energy storage materials synthesized by many bacteria.PHA,as a kind of degradable material,can be used in many areas,such as bioplastics,medical materials etc.In this study,several soil or water samples were collected from environment,and a polyhydroxyalkanoate producing strain was isolated from soil by enrichment culturing,Nile blue fluorescence staining,Sudan black staining,and Fourier transform infrared spectra elemental analysis.This strain was identified and named as Rhizobium sp.H2-5 according to its morphological features,physiological and biochemical tests as well as 16S rDNA gene sequence analysis.
{"title":"Screening and Identification of a Polyhydroxyalkanoate Producing Strain Rhizobium sp. H2-5","authors":"Yang Shanshan, Zhang Hongrui, Fu Sheng-mi, Li Xinli, C. Jing","doi":"10.3724/SP.J.1145.2013.00375","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00375","url":null,"abstract":"Polyhydroxyalkanoate(PHA) is a family of carbon and energy storage materials synthesized by many bacteria.PHA,as a kind of degradable material,can be used in many areas,such as bioplastics,medical materials etc.In this study,several soil or water samples were collected from environment,and a polyhydroxyalkanoate producing strain was isolated from soil by enrichment culturing,Nile blue fluorescence staining,Sudan black staining,and Fourier transform infrared spectra elemental analysis.This strain was identified and named as Rhizobium sp.H2-5 according to its morphological features,physiological and biochemical tests as well as 16S rDNA gene sequence analysis.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69809149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00444
R. Hu, Shu Zhang, Jian-wei Wu, Guo Guo, P. Fu
For understanding whether or not Musca domestica has an ability to hydrolyze cellulose,the activities and characteristics of cellulases from M.domestica were examined with the 3,5-dinitrosalicylic acid(DNSA) method.Then the composition and activity change of M.domestica cellulases were investigated during its development.The results showed that M.domestica in the different developmental stages had the filter paper enzyme activity,the activity-range being 0.85(± 0.035) IU mg-1to 10.45(± 0.050) IU mg-1.The cellulase components included β-glucosidase(BG),endo-β-1,4 glucanase(EG) and exoβ-1,4-glucanase(CBH),which were detected with the special substrate of 1% salicin,1% carboxymethylcellulose(CMC) and 1% microcrystalline cellulose(MCC),respectively.Under the condition of pH 5.6,for the three enzymes,the optimum reaction temperature and time were 50 ℃ and 60 min.They had good heat stability in vitro when being kept in 50 ℃ for 1 hour.Activity of the cellulases increased gradually with its development(P 0.05).The lowest enzyme activity showed in the ovule stage and the highest enzymatic activity in the imago stage.At the optimum reaction condition,the activities of BG,EG and CBH were 7.96(± 0.065) IU mg-1,9.76(± 0.080) IU mg-1 and 10.86(± 0.091) IU mg-1 respectively.The CBH activity was the highest,and the BG the lowest in M.domestica life cycle.With increasing crude fiber in the forage of M.domestica larvae,cellulase activity of the third instar larvae was also increased(P 0.05),without a significant deviation of mean weight and stem length of the third instar larvae compared to the control group(P 0.05).The results suggested a complete enzyme system of cellulases in M.domestica and a close relationship between the enzyme activity level and the development of M.domestica.
{"title":"Q969.453.806Detection of Cellulases and the Relationship Between Enzyme Activity and Development of Musca domestica : Q969.453.806Detection of Cellulases and the Relationship Between Enzyme Activity and Development of Musca domestica","authors":"R. Hu, Shu Zhang, Jian-wei Wu, Guo Guo, P. Fu","doi":"10.3724/SP.J.1145.2013.00444","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00444","url":null,"abstract":"For understanding whether or not Musca domestica has an ability to hydrolyze cellulose,the activities and characteristics of cellulases from M.domestica were examined with the 3,5-dinitrosalicylic acid(DNSA) method.Then the composition and activity change of M.domestica cellulases were investigated during its development.The results showed that M.domestica in the different developmental stages had the filter paper enzyme activity,the activity-range being 0.85(± 0.035) IU mg-1to 10.45(± 0.050) IU mg-1.The cellulase components included β-glucosidase(BG),endo-β-1,4 glucanase(EG) and exoβ-1,4-glucanase(CBH),which were detected with the special substrate of 1% salicin,1% carboxymethylcellulose(CMC) and 1% microcrystalline cellulose(MCC),respectively.Under the condition of pH 5.6,for the three enzymes,the optimum reaction temperature and time were 50 ℃ and 60 min.They had good heat stability in vitro when being kept in 50 ℃ for 1 hour.Activity of the cellulases increased gradually with its development(P 0.05).The lowest enzyme activity showed in the ovule stage and the highest enzymatic activity in the imago stage.At the optimum reaction condition,the activities of BG,EG and CBH were 7.96(± 0.065) IU mg-1,9.76(± 0.080) IU mg-1 and 10.86(± 0.091) IU mg-1 respectively.The CBH activity was the highest,and the BG the lowest in M.domestica life cycle.With increasing crude fiber in the forage of M.domestica larvae,cellulase activity of the third instar larvae was also increased(P 0.05),without a significant deviation of mean weight and stem length of the third instar larvae compared to the control group(P 0.05).The results suggested a complete enzyme system of cellulases in M.domestica and a close relationship between the enzyme activity level and the development of M.domestica.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69809646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00249
Xia Li, T. He, Ze Chun
{"title":"Detecting Genetic Diversity of Dendrobium Tissue Culture Seedlings with ISSR Method: Detecting Genetic Diversity of Dendrobium Tissue Culture Seedlings with ISSR Method","authors":"Xia Li, T. He, Ze Chun","doi":"10.3724/SP.J.1145.2013.00249","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00249","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00205
Shengxiong Huang, Yongsheng Liu
The WRKY transcription factor family is widely involved in regulating plant development and defense response against various biotic and abiotic stresses.The Hidden Markov Model Profile(HMM) of WRKY domain was used to identify WRKY transcription factors in potato genome with HMMER 3.0.All together 81 potato WRKY transcription factors were identified and then classified into three main groups,with 5 subgroups in Group Ⅱ,namely Ⅱ-a,Ⅱ-b,Ⅱ-c,Ⅱ-d,and Ⅱ-e.The constructed phylogenetic tree of WRKY genes among potato,Arabidopsis and rice demonstrated that the distinct gene expansion events occurred in the genome of potato and rice,respectively,while the gene expansion of Group Ⅱ-e was unique in potato genome.The potato WRKY transcription factors showed similar motif compositions within each group,but different ones among different groups,though several conserved motifs were shared by all.Furthermore,28 potato WRKY genes showed distinct expression patterns in response to stresses of wounding,drought,salt,and virus invasion.The expressions of several potato WRKY genes were significantly up/down-regulated,implying that these members might participate in regulating the defense response against various biotic and abiotic stresses.
{"title":"Genome-wide Analysis of WRKY Transcription Factors in Solanum tuberosum","authors":"Shengxiong Huang, Yongsheng Liu","doi":"10.3724/SP.J.1145.2013.00205","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00205","url":null,"abstract":"The WRKY transcription factor family is widely involved in regulating plant development and defense response against various biotic and abiotic stresses.The Hidden Markov Model Profile(HMM) of WRKY domain was used to identify WRKY transcription factors in potato genome with HMMER 3.0.All together 81 potato WRKY transcription factors were identified and then classified into three main groups,with 5 subgroups in Group Ⅱ,namely Ⅱ-a,Ⅱ-b,Ⅱ-c,Ⅱ-d,and Ⅱ-e.The constructed phylogenetic tree of WRKY genes among potato,Arabidopsis and rice demonstrated that the distinct gene expansion events occurred in the genome of potato and rice,respectively,while the gene expansion of Group Ⅱ-e was unique in potato genome.The potato WRKY transcription factors showed similar motif compositions within each group,but different ones among different groups,though several conserved motifs were shared by all.Furthermore,28 potato WRKY genes showed distinct expression patterns in response to stresses of wounding,drought,salt,and virus invasion.The expressions of several potato WRKY genes were significantly up/down-regulated,implying that these members might participate in regulating the defense response against various biotic and abiotic stresses.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00318
Huaju Yang, Jun Huang, Yonggui Zhao, Guoke Chen
{"title":"Identification and Characterization of Thauera sp. Strain TN9: Identification and Characterization of Thauera sp. Strain TN9","authors":"Huaju Yang, Jun Huang, Yonggui Zhao, Guoke Chen","doi":"10.3724/SP.J.1145.2013.00318","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00318","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-19DOI: 10.3724/SP.J.1145.2013.00449
Z. Tan, Hualing Zhang, Sainan Zhou, Wang Yu, Ao Zeng, Ying-Li Cai, Guangxian Cai
{"title":"R285.5Change of Intestinal Microbes in Dysbacteriosis-modeled Mice Treated with Ultra-micro Powder of Qiweibaizhusan: R285.5Change of Intestinal Microbes in Dysbacteriosis-modeled Mice Treated with Ultra-micro Powder of Qiweibaizhusan","authors":"Z. Tan, Hualing Zhang, Sainan Zhou, Wang Yu, Ao Zeng, Ying-Li Cai, Guangxian Cai","doi":"10.3724/SP.J.1145.2013.00449","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00449","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69809263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Association Analysis Between Forest Stand Traits and Habitat Conditions in Picea schrenkiana var. tianschanica Forest in Tianshan Mountains: Association Analysis Between Forest Stand Traits and Habitat Conditions in Picea schrenkiana var. tianschanica Forest in Tianshan Mountains","authors":"Baiketuerhan Yeerjiang, Chunyu Zhang, Xiu-hai Zhao","doi":"10.3724/SP.J.1145.2013.00410","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00410","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69809354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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