Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00519
Xingbiao Wang, Yue-qin Tang, Gang Wu, Xiaolei Wu
This experiment optimized the extraction and detection methods of biosurfactant produced by the strain Dietzia sp. DQ12-45-1b, and studied biosurfactant products of the strain growing on n-hexadecane for different cultural time. Ethyl acetate was the best extraction solvent with the highest metabolite recovery effi cient. The mixture of hexane : ammonia : isopropanol = 2:1:4 (V:V:V) was used as the best solvent system in thin layer chromatography analysis. The strain DQ12-45-1b produced glycolipid biosurfactant, with the biosurfactant concentration of the culture broth positively correlated with the incubation time. The content ratio of crude extracts in emulsifi ed hexadecane layer and aqueous medium was 4.16:1. A glycolipid was detected in the emulsifi ed hexadecane layer but not in aqueous phase. Fig 5, Tab 1, Ref 15
{"title":"O658Detection of Metabolites from n -Hexadecane Degradation by Strain Dietzia sp. DQ12-45-1b: O658Detection of Metabolites from n -Hexadecane Degradation by Strain Dietzia sp. DQ12-45-1b","authors":"Xingbiao Wang, Yue-qin Tang, Gang Wu, Xiaolei Wu","doi":"10.3724/SP.J.1145.2013.00519","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00519","url":null,"abstract":"This experiment optimized the extraction and detection methods of biosurfactant produced by the strain Dietzia sp. DQ12-45-1b, and studied biosurfactant products of the strain growing on n-hexadecane for different cultural time. Ethyl acetate was the best extraction solvent with the highest metabolite recovery effi cient. The mixture of hexane : ammonia : isopropanol = 2:1:4 (V:V:V) was used as the best solvent system in thin layer chromatography analysis. The strain DQ12-45-1b produced glycolipid biosurfactant, with the biosurfactant concentration of the culture broth positively correlated with the incubation time. The content ratio of crude extracts in emulsifi ed hexadecane layer and aqueous medium was 4.16:1. A glycolipid was detected in the emulsifi ed hexadecane layer but not in aqueous phase. Fig 5, Tab 1, Ref 15","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"519-522"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00471
X. Luan, J. Duan, Yong-Jun Chen, Z. Chi
{"title":"X55Diversity of Bacterial Community on the Surface of Marine Paint in Temperate Coastal Marine Waters: X55Diversity of Bacterial Community on the Surface of Marine Paint in Temperate Coastal Marine Waters","authors":"X. Luan, J. Duan, Yong-Jun Chen, Z. Chi","doi":"10.3724/SP.J.1145.2013.00471","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00471","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"471-477"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00495
Wei Wang, K. Qu, Jian-bing Zhu, Z. Cui, Cuihua Yang, Haizeng Wang
{"title":"Effect of Carbon Sources on Bio-film Cultivation and Its Nitrification-denitrification Performance: Effect of Carbon Sources on Bio-film Cultivation and Its Nitrification-denitrification Performance","authors":"Wei Wang, K. Qu, Jian-bing Zhu, Z. Cui, Cuihua Yang, Haizeng Wang","doi":"10.3724/SP.J.1145.2013.00495","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00495","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"16 1","pages":"495-500"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00547
Yinyin Liu, Feng Li, Qingye Sun, Yonghong Xie
{"title":"Review on the Study of Soil Microorganisms in Wetland Ecosystems: Review on the Study of Soil Microorganisms in Wetland Ecosystems","authors":"Yinyin Liu, Feng Li, Qingye Sun, Yonghong Xie","doi":"10.3724/SP.J.1145.2013.00547","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00547","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"547-552"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00506
Jingjing Cao, Yonghui Li, Yucheng Li, Juan Wu, Ning Wang, Yueqin Dou
{"title":"Isolation of a Humus-reducing Bacterium Strain and Its Biodegradation of Pollutants: Isolation of a Humus-reducing Bacterium Strain and Its Biodegradation of Pollutants","authors":"Jingjing Cao, Yonghui Li, Yucheng Li, Juan Wu, Ning Wang, Yueqin Dou","doi":"10.3724/SP.J.1145.2013.00506","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00506","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00528
Peiyong Song, Lin Li, Zhongjiu Xiao, Xiaoxia Li
{"title":"2.3Identification and Phylogenetic Analysis of Four Endophytic Bacteria Isolated from Alsophila spinulosa : 2.3Identification and Phylogenetic Analysis of Four Endophytic Bacteria Isolated from Alsophila spinulosa","authors":"Peiyong Song, Lin Li, Zhongjiu Xiao, Xiaoxia Li","doi":"10.3724/SP.J.1145.2013.00528","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00528","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"528-531"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00537
C. Luo, T. He, Ze Chun
{"title":"Advances in Infrared Spectroscopy Methods for Discriminating Dendrobium : Advances in Infrared Spectroscopy Methods for Discriminating Dendrobium","authors":"C. Luo, T. He, Ze Chun","doi":"10.3724/SP.J.1145.2013.00537","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00537","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"537-541"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-20DOI: 10.3724/SP.J.1145.2013.00515
Xingbiao Wang, Qing Zhang, Yang Liu, Yue-qin Tang, Gang Wu, Xiaolei Wu
Abstract This study analyzed the microbial community structure, microorganisms and the oil-degrading activities in the dehydrated crude oil from Daqing Oil fi eld. The results showed the existence of many microbial cells in the dehydrated crude oil, and screened a microbial community with oil-emulsifying and oil-dispersing activity. Community analysis showed that the types and rations of the microorganisms in the community changed with incubation time. All together 166 bacteria were isolated and puri fi ed from the crude oil culture system, up to 13.1% of the tested strains having oil-dispersing or oil-emulsifying activities. The results showed that there are microbial communities with oil emulsifying and oil dispersing activities in Daqing oil reservoir, that the harsh conditions and the lack of nutrition leads to smaller community density, and that the oil degrading strains occupy a high proportion.
{"title":"CLC Q938.15 : X74Microorganisms and Their Oil-degrading Activities in Dehydrated Crude Oil: CLC Q938.15 : X74Microorganisms and Their Oil-degrading Activities in Dehydrated Crude Oil","authors":"Xingbiao Wang, Qing Zhang, Yang Liu, Yue-qin Tang, Gang Wu, Xiaolei Wu","doi":"10.3724/SP.J.1145.2013.00515","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00515","url":null,"abstract":"Abstract This study analyzed the microbial community structure, microorganisms and the oil-degrading activities in the dehydrated crude oil from Daqing Oil fi eld. The results showed the existence of many microbial cells in the dehydrated crude oil, and screened a microbial community with oil-emulsifying and oil-dispersing activity. Community analysis showed that the types and rations of the microorganisms in the community changed with incubation time. All together 166 bacteria were isolated and puri fi ed from the crude oil culture system, up to 13.1% of the tested strains having oil-dispersing or oil-emulsifying activities. The results showed that there are microbial communities with oil emulsifying and oil dispersing activities in Daqing oil reservoir, that the harsh conditions and the lack of nutrition leads to smaller community density, and that the oil degrading strains occupy a high proportion.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"515-518"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Intestinal Microbes on the Immune System and the Latest Research Methods: Effect of Intestinal Microbes on the Immune System and the Latest Research Methods","authors":"Dandan Chen, Shenghua Gu, Jinna Zhang, Fukai Bao, Yue-qin Tang, Xiaolei Wu","doi":"10.3724/SP.J.1145.2013.00542","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00542","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"542-546"},"PeriodicalIF":0.0,"publicationDate":"2013-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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