Pub Date : 2023-12-25DOI: 10.26577/ijbch2023v16i2a2
Tokpayev E.R, Shreider, Z. T. Ibraimov, T. M. Shalakhmetova, M. K. Nauryzbayev
The use of pig bone tissue as a graft makes it possible to obtain high-quality, relatively cheap and biocompatible ECM. This is since the mineral composition of pork bone is most similar to the mineral composition of human bone. Decellularization in SC-CO2 is a promising direction, since carbon dioxide easily diffuses into the depth of the cell and is a good solvent for lipids, it is non-flammable, non-toxic and chemically non-aggressive. In this paper, the ECM in SC-CO2 was obtained in three ways: 1) in dynamic mode with large SC-CO2 flows; 2) in static and dynamic modes with large SC-CO2 flows; 3) in static and dynamic modes with large SC-CO2 flows, with preliminary exposure in ethyl alcohol as a co-solvent. According to the histological examination, the removal of ICC by the first method is 55%. The use of a co-solvent before starting the decellularization process increases the percentage of ICC removal to 98%, which allows the use of ECM as a transplant.
{"title":"Decellularization of bone tissue in a supercritical carbon dioxide environment","authors":"Tokpayev E.R, Shreider, Z. T. Ibraimov, T. M. Shalakhmetova, M. K. Nauryzbayev","doi":"10.26577/ijbch2023v16i2a2","DOIUrl":"https://doi.org/10.26577/ijbch2023v16i2a2","url":null,"abstract":"The use of pig bone tissue as a graft makes it possible to obtain high-quality, relatively cheap and biocompatible ECM. This is since the mineral composition of pork bone is most similar to the mineral composition of human bone. Decellularization in SC-CO2 is a promising direction, since carbon dioxide easily diffuses into the depth of the cell and is a good solvent for lipids, it is non-flammable, non-toxic and chemically non-aggressive. In this paper, the ECM in SC-CO2 was obtained in three ways: 1) in dynamic mode with large SC-CO2 flows; 2) in static and dynamic modes with large SC-CO2 flows; 3) in static and dynamic modes with large SC-CO2 flows, with preliminary exposure in ethyl alcohol as a co-solvent. According to the histological examination, the removal of ICC by the first method is 55%. The use of a co-solvent before starting the decellularization process increases the percentage of ICC removal to 98%, which allows the use of ECM as a transplant.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"32 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.26577/ijbch2023v16i2a4
A. H.M.U.Shaheen, Rajput, L. Atiq, Amrao W.R, Arshad G.A, Kachelo, M. Usman, M. F. Tahir
Rice (Oryzae sativa L.) is suffering from several biotic and abiotic factors. Among biotic factors, brown leaf spot of rice (BLS) is potentially devastating disease of rice causing the severe yield losses up to 100%. The current study was designed to evaluate most effective synthetic fungicides management strategy towards BLS disease. For this purpose, 12 fungicides were screened out under in vitro conditions and four most effective fungicides were further demonstrated against the targeted pathogen with three different concentrations (100, 150 and 150ppm) by using poisoned food technique. The results revealed that propiconazole and thiophanate methyl showed the strong inhibitory effect against B. oryzae followed by contaf plus and bavistin respectively. The promising fungicides (propiconazole and thiophanate methyl) under lab conditions were further evaluated in vivo against BLS disease by using 3 types of applications i.e. Pre-inoculation, Post-inoculation and after symptoms appearance. Findings showed that, Post-inoculation was found most effective as compared to other applications, furthermore combination of propiconazole and thiophanate methyl showed significant reduction in disease incidence percent as compared to solo applications. The reduction in disease incidence percent in all application methods suggested that these synthetic fungicides could be used against BLS of rice.
{"title":"Synthetic fungicides for controlling brown leaf spot of rice caused by Bipolaris oryzae","authors":"A. H.M.U.Shaheen, Rajput, L. Atiq, Amrao W.R, Arshad G.A, Kachelo, M. Usman, M. F. Tahir","doi":"10.26577/ijbch2023v16i2a4","DOIUrl":"https://doi.org/10.26577/ijbch2023v16i2a4","url":null,"abstract":"Rice (Oryzae sativa L.) is suffering from several biotic and abiotic factors. Among biotic factors, brown leaf spot of rice (BLS) is potentially devastating disease of rice causing the severe yield losses up to 100%. The current study was designed to evaluate most effective synthetic fungicides management strategy towards BLS disease. For this purpose, 12 fungicides were screened out under in vitro conditions and four most effective fungicides were further demonstrated against the targeted pathogen with three different concentrations (100, 150 and 150ppm) by using poisoned food technique. The results revealed that propiconazole and thiophanate methyl showed the strong inhibitory effect against B. oryzae followed by contaf plus and bavistin respectively. The promising fungicides (propiconazole and thiophanate methyl) under lab conditions were further evaluated in vivo against BLS disease by using 3 types of applications i.e. Pre-inoculation, Post-inoculation and after symptoms appearance. Findings showed that, Post-inoculation was found most effective as compared to other applications, furthermore combination of propiconazole and thiophanate methyl showed significant reduction in disease incidence percent as compared to solo applications. The reduction in disease incidence percent in all application methods suggested that these synthetic fungicides could be used against BLS of rice.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"2 10","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139157960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.26577/ijbch2023v16i2a15
D. Narin, Y. Cosgun, G. Korukluoglu, M. Kavutcu
The aim of this study was to investigate the serum neopterin level and ADA activity in acute measles infection and determine whether there is a correlation between the measles optical density values, and the neopterin and ADA levels. The neopterin level and ADA activities were investigated in the samples of 136 measles IgM-positive patients along with those of 40 measles IgM-negative patients as the control group. The most important findings of the study was the determination of significantly higher neopterin levels and ADA activity in the measles IgM-positive group when compared to the measles IgM-negative group. These findings have shown that the neopterin level and ADA activity can assist in the diagnosis of measles in the acute period as biomarkers.
本研究旨在调查急性麻疹感染者的血清蝶呤水平和 ADA 活性，并确定麻疹光密度值与蝶呤和 ADA 水平之间是否存在相关性。研究人员对 136 名麻疹 IgM 阳性患者和 40 名麻疹 IgM 阴性患者作为对照组的样本中的新蝶呤水平和 ADA 活性进行了调查。该研究最重要的发现是，与麻疹 IgM 阴性组相比，麻疹 IgM 阳性组的新蝶呤水平和 ADA 活性明显更高。这些研究结果表明，新蝶呤水平和 ADA 活性作为生物标志物可以帮助诊断急性期麻疹。
{"title":"Investigation of serum neopterin levels and adenosine deaminase enzymatic activity in measles infection","authors":"D. Narin, Y. Cosgun, G. Korukluoglu, M. Kavutcu","doi":"10.26577/ijbch2023v16i2a15","DOIUrl":"https://doi.org/10.26577/ijbch2023v16i2a15","url":null,"abstract":"The aim of this study was to investigate the serum neopterin level and ADA activity in acute measles infection and determine whether there is a correlation between the measles optical density values, and the neopterin and ADA levels. The neopterin level and ADA activities were investigated in the samples of 136 measles IgM-positive patients along with those of 40 measles IgM-negative patients as the control group. The most important findings of the study was the determination of significantly higher neopterin levels and ADA activity in the measles IgM-positive group when compared to the measles IgM-negative group. These findings have shown that the neopterin level and ADA activity can assist in the diagnosis of measles in the acute period as biomarkers.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"19 6","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139157555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.26577/ijbch.2023.v16.i1.07
Y. Syrgabek
{"title":"Miniaturized solid-phase microextraction of caffeine in tea samples: assessing the environmental friendliness of the methods with the GAPI tool","authors":"Y. Syrgabek","doi":"10.26577/ijbch.2023.v16.i1.07","DOIUrl":"https://doi.org/10.26577/ijbch.2023.v16.i1.07","url":null,"abstract":"","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45549518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.26577/ijbch.2023.v16.i1.012
M. Zhumagul
{"title":"Component composition of essential oil of Rhodiola algida (Ledeb.) Fisch & C.A. Mey and its biological activity","authors":"M. Zhumagul","doi":"10.26577/ijbch.2023.v16.i1.012","DOIUrl":"https://doi.org/10.26577/ijbch.2023.v16.i1.012","url":null,"abstract":"","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47951789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.26577/ijbch.2023.v16.i1.05
E. Djangalina
{"title":"Influence of light-emitting diodes on the efficiency of valuable woody plants micropropagation","authors":"E. Djangalina","doi":"10.26577/ijbch.2023.v16.i1.05","DOIUrl":"https://doi.org/10.26577/ijbch.2023.v16.i1.05","url":null,"abstract":"","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42792818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.26577/ijbch.2023.v16.i1.08
A. Battalova
{"title":"Features of microcrystalline cellulose produced from sunflower seeds of different oil content","authors":"A. Battalova","doi":"10.26577/ijbch.2023.v16.i1.08","DOIUrl":"https://doi.org/10.26577/ijbch.2023.v16.i1.08","url":null,"abstract":"","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43388409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.26577/ijbch.2023.v16.i1.04
A. Kapytina
{"title":"Genetic resistance of pear cultivars to fire blight","authors":"A. Kapytina","doi":"10.26577/ijbch.2023.v16.i1.04","DOIUrl":"https://doi.org/10.26577/ijbch.2023.v16.i1.04","url":null,"abstract":"","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45879688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.26577/ijbch.2023.v16.i1.02
Lamiaa Elsayed Mokhtar Deef
{"title":"Molecular phylogenetics of some endangered turtles reveals new close genetic relationships","authors":"Lamiaa Elsayed Mokhtar Deef","doi":"10.26577/ijbch.2023.v16.i1.02","DOIUrl":"https://doi.org/10.26577/ijbch.2023.v16.i1.02","url":null,"abstract":"","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48079401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.26577/ijbch.2023.v16.i1.011
M. M. Torekhanova
{"title":"Study of the possibility of using agricultural wastewater for the accumulation of microalgae biomass.","authors":"M. M. Torekhanova","doi":"10.26577/ijbch.2023.v16.i1.011","DOIUrl":"https://doi.org/10.26577/ijbch.2023.v16.i1.011","url":null,"abstract":"","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45429659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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