Chinexe Journal of Pediatrics最新文献

{"title":"A prospective randomized trial comparing intermittent nasoduodenal with intermittent nasogastric feedings in premature infants","authors":"Hui Lu, Xindong Xue, Jiaxiang Zhang, Pengfei Li","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.04.105","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.04.105","url":null,"abstract":"Objective To compare and evaluate the effects of intermittent nasoduodenal feeding (INDF) and intermittent nasogastric feeding (INGF) on nutrient intake, physical growth, gut transit time and whole gastrointestinal transit time and feeding related complications at the early time after birth in premature infants Methods Forty premature infants (birth weights ranged from 1 050 g to 1 920 g) were randomized into INDF and INGF groups and fed with the same formula Intake of fluid (including both intravenous and oral), caloric intake, protein intake, variation of physical growth parameters (e g, body weight, length and head circumference), stool characters, and relevant complications were recorded; gut transit time and whole gastrointestinal transit time were monitored Results Within a week after feeding, milk input was (67 2±38 8) ml/(kg·d), (55 7±36 6) ml/(kg·d), respectively, in INDF and INGF groups The caloric intake and the protein intake supplied by milk were (217 1±125 5) kJ/(kg·d) vs (188 7±126 4) kJ/(kg·d) and (1 62±0 9) g/(kg·d) vs (1 22±0 82) g/(kg·d) The nutrient intake in INDF group was significantly higher than that in INGF group ( P 0 001) The time of reaching 418 4 kJ/(kg·d) by enteral feeding were respectively (9 3±1 9) d and (13 9±7 4) d , the birth weight regaining time were (8 4±1 8) d vs (10 2±2 5) d; all these parameters in INDF group were significantly lower than those in INGF group ( P 0 05) There was no significant difference in the increase of length and head circumference between the two groups ( P 0 05) The gut transit time [INDF, (53 3±14 0) h] was significantly shorter than the whole gastrointestinal transit time [INGF, (63 2±14 9) h], but there was no such complications as diarrhea, in the two groups The morbidities of such complications as apnea and pneumonia caused by aspiration, vomiting, gastric residue were lower in INDF group than those in INGF group, but the difference was not statistically significant ( P 0 05) However, the morbidity of hyperbilirubinemia was significantly lower in INDF than that in INGF group ( P 0 01) Conclusion Nasoduodenal feeding was more tolerable than nasogastric feeding in the initial feeding of premature infants","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131838243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of clinical date of 620 very low birth weight infants","authors":"Li-ping Shi, L. Du","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.04.104","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.04.104","url":null,"abstract":"The rapid decline in mortality of very low birth weight infants (VLBWI) during the past twenty years has been attributed to the establishment of the neonatal intensive care all over the country. Little information was reported on the conditions of VLBWI during the recent few years in China. \u0000 \u0000 \u0000Objective \u0000To investigate the factors in relation to the improved hospital survival rate of VLBWI. \u0000 \u0000 \u0000Methods \u0000The hospital data of 620 VLBWI during the last ten years were analyzed by dividing patients into three groups according to the birth weight, that is, less than 1 000 grams, between 1 001 and 1 250 grams and between 1 251 and 1 500 grams, respectively. The risk factors, the effects of these factors and mortality 1991~1995 and 1996~2000 were analyzed and compared. \u0000 \u0000 \u0000Results \u0000The risk factors causing the premature birth of VLBWI were multiple births (27.3%), pre-rupture of membrane (20.2%), and pregnancy complications (11.5%). In 36.8% cases no reason could be found. All hospitalized VLBWI had at least one complication including apnea (61.9%), which happened at 78.8%, 79.1% and 52.6%, respectively in the three groups; hypothermia (56.1%), at 65.2%, 62.1% and 52.4%, respectively in the three groups; intraventricular hemorrhage (IVH) (41.9%), at 80.3%, 62.1% and 27.9%, respectively in three groups; respiratory distress syndrome (RDS, 35.8%), at 62.1%, 44.4% and 28.2%, respectively in the three groups; patent ductus arteriosus (PDA, 27.2%), at 33.3 %, 26.8% and 26.2%, respectively in three groups and, infection (26.1%), at 27.3%, 37.3% and 9.2%, respectively in the three groups. The highest frequency of complications occurred in the babies with birth weight less than 1 000 grams. The survival rate was 86.5% for all VLBWI and 60.6% for babies less than 1 000 grams. In recent years, the survival rate has been improved significantly (80.8% vs 89.2%, χ2=8.27, P<0.005). RDS, infection and IVH were the primary causes of death. \u0000 \u0000 \u0000Conclusions \u0000The declines in the mortality of VLBWI were attributed to the improved neonatal intensive care, continuous post-natal monitoring and the early finding of complications. Early treatment of VLBWI could increase the survival rate. The prevention of infections, especially in nosocomial infection is one of the most important steps of decreasing mortality. Improved survival rate in RDS may be associated with the introduction of surfactant replacement therapy and high-frequency ventilation. \u0000 \u0000 \u0000Key words: \u0000Infant, very low birht weight; Survival rate; Retrospective studies","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133253193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential diagnostic significance of cytokines in cerebrospinal fluid of children with acute central nervous system infections","authors":"Yu Jin, J. Xue, Wei-jun Yu","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.04.103","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.04.103","url":null,"abstract":"Objective To investigate the diagnostic value of cytokines in central nervous system infection (CNSI), the levels of interferon gamma (IFN γ), granulocyte colony stimulating factor (G CSF), tumor necrosis factor α(TNF α), interleukin 6 (IL 6) and interleukin 8 (IL 8) in the cerebrospinal fluid (CSF) were measured in the children with CNSI of different etiology Methods The concentrations of IFN γ, G CSF, TNF α, IL 6 and IL 8 were measured in the CSF of 66 children, of whom 14 patients had purulent meningitis (PM), 30 patients had acute viral meningitis (VM), and 22 were control children without CNSI The ages were from 5 months to 13 years The concentrations of cytokines were detected by enzyme linked immunosorbent assay (ELISA) The CSF specimens were analyzed for the white blood cell count, glucose and the concentrations of IFN γ, G CSF, TNF α, IL 6 and IL 8 Results The CSF levels of G CSF and IL 8 were significantly higher in the PM group [(132±41), (2 53±0 56) μg/L] than in the VM group [ (53±22), (0 53±0 46) μg/L] and control group [(41±24), (0 38±0 29) μg/L, P 0 001] The IFN γ concentration was markedly higher in the CSF of patients with VM [(2 0±0 4) μg/L] than patients with PM [(0 8±0 5) μg/L, P 0 01] and control group [(0 6±0 5) μg/L, P 0 001] The TNF α and IL 6 concentrations were obviously higher in the PM group [(3 21±1 36) μg/L] and VM group [(2 23±1 05) μg/L] compared with the control group [(0 45±0 23) μg/L, P 0 001] No significant difference was found between PM group and VM group in TNF α and IL 6 levels in CSF The concentrations of G CSF and IL 8 in the CSF were positively correlated to the neutrophil count ( r =0 182, P =0 022; r =0 201, P =0 032) The IFN γ, TNF α and IL 6 concentrations were not correlated to the neutrophil count, glucose and protein amounts in CSF( r =0 087, r =0 075, r =0 122, P 0 10) Conclusion The cytokines increased in the CSF of patients with CNSI It may contribute to the pathophysiological mechanisms of acute infection of the central nervous system The CSF levels of G CSF and IL 8 may be helpful to distinguish PM from VM The IFN γ concentration in the CSF may be valuable in differential diagnosis between VM and PM The CSF levels variation of TNF α and IL 6 were not significant in the differential diagnosis of CNSI","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131060724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey of β-Mediterranean anemia (β-TA) prevalence in ethnic minority She of the southwest area in Zhejiang province","authors":"Lihua Liu, Ying Chen, You-Ming Ding, Chenfu Lan","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.04.108","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.04.108","url":null,"abstract":"Objective \u0000There are eighty thousand ethnic minority She people in the southwest area of Zhejiang province, which accounts for 12.5 per cent of She in China. Since there is no report on β-TA in She people, this study aimed at exploring the morbidity of β-TA in She population in this area and related hereditary factors. \u0000 \u0000 \u0000Methods \u0000Lishui district were selected, because 70 per cent of the inhabitants were She people. Nineteen counties of Lishui city were included, such as Yunhe county, Jingning county, Suichang county, Songyang county, Longquan county and so on. Five folk primary and high schools and 17 general primary and high schools from forty-two natural villages as well as in Lishui city were surveyed. Ninety per cent of them were located in remote counties. Totally 1 650 cases were sampled by multiple-degree sampling survey, 1 391cases were by total sampling survey and 424 cases were by supplement survey. Totally 3 465 persons were surveyed, among whom 1 561 were males (45.1%), 1 904 were females (54.9%). There were 483 people aged from 0 to 10 years (13.9%), 1 634 aged -20 years (47.2%), 212 aged -30 years (6.1%), 402 aged -40 years (11.6%), 302 aged -50 years (8.7%), and 432 aged -51 years or older (12.5%). The survey process was as follows, firstly, specimen were anti-coagulated by EDTA-K2 reagent and tested for RBC analysis by Counter -JT3 automatic blood cell analyzer. Secondly, the hemoglobin electrophoresis was performed. All the cases were tested with the automatic blood cell analyzer. There were 316 cases with microcytic and/or hypochromic anemia tested by the hemoglobin electrophoresis. Thirdly, 34 of 48 family members from 5 unrelated probands with intermediate advanced congenital β-TA were surveyed for hereditary genealogy. Fourthly, liquids extracted from white blood cells of people who had had survey of hereditary genealogy and part of those with microcytic hypochromic anemia were tested with the methods of reverse-cross testing (RDB) for the type of gene mutation of β-TA and with PCR for the type of gene deficiency of α-TA. \u0000 \u0000 \u0000Results \u0000Among 3 465 inhabitants, 316 cases were found to have microcytic hypochromic anemia, 235 cases were abnormal and diagnosed with the hemoglobin electrophoresis. Of the 211 abnormal cases, 200 cases showed HbA2>3.5%, 35 cases HbF>1%, which indicated that the morbidity in this area was 6.78%. Tested in the same way, there were 18 cases and 6 cases respectively in 34 cases with congenital β-TA, which showed that there was conglomerate family mobility of β-TA . The relationship among HbA2, HbF and MCV in 235 cases with β-TA showed that MCV≤81f l (91.5%) in 215 cases and MCV≥81fl in 20 cases (8.5%). Most of the 20 cases belonged to the congenital β-TA family members, of whom 2 children had advanced β-TA with the history of splenectomy and splenic embolism, respectively. The two children also showed increased MCV, markedly higher RDW (RBC volume distribution width) and abnormal bell-shaped graph fo","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133917692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of bronchoalveolar lavage fluid components in children with chronic cough and wheezy diseases","authors":"A. Jiao, Xi-cheng Liu, Qin-bo Jiang, Shunying Zhao","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.02.111","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.02.111","url":null,"abstract":"Objective \u0000Today the technique of bronchoalveolar lavage (BAL ) has been widely used as a useful research tool in studying the airway inflammation of airway diseases by investigating inflammatory cells and inflammatory mediators in bronchoalveolar lavage fluid (BALF ) in adults, but there have been very few the similar studies in pediatric cases. The purpose of this prospective study was to analyze the components of BALF in children with chronic cough and wheezy diseases. \u0000 \u0000 \u0000Methods \u0000Fiberoptic bronchoscopy was performed on 39 children aged 1 year to 11 years, who were divided into 4 groups with regard to clinical data: asthma (n=13), chronic cough (n=10), infantile wheezing (n= 8), and control (n= 8). BAL was performed with 3×1 ml/kg body weight of normal saline warmed to body temperature. Differential cell counts were obtained from smears stained with May-Grunwald-Giemsa method. Activated eosinophils and T cells were marked with EG2 and CD45RO monoclonal antibodies, respectively, using immunohistochemical method. Interleukin-5 (IL-5) was measured with a sandwich ELISA using a mouse monoclonal antibody. Statistical analysis was conducted with the SPSS 8.0 software. P< 0.05 was considered statistically significant. \u0000 \u0000 \u0000Results \u0000The mean (±s) recovery of BALF was (77±6)% and no difference was found between the groups. Eosinophils 3.0%(0.7%-8.8%), EG2-positive cells 2.0% (0.8%-7.5%), and epithelial cells 3.0% (0.7%-12.0%) in BALF were significantly increased in children with asthma by comparison with chronic cough, infantile wheezing and control subjects (P< 0.01, respectively). The cell profile of children with chronic cough was similar to that of control children, but increased eosinophils and EG2-positive cells were also found in two of them. Activated T cell (CD45RO+ ) counts were significantly increased in asthmatic children as compared with controls (P< 0.05 ) but no significant difference was found when compared with children with chronic cough and infantile wheezing. Neutrophils were significantly increased in asthmatic children and infantile wheezers in comparison with controls (P<0.05, respectively). IL-5 (1.6 ng/L; 0, 16.0 ng/ml) levels were significantly increased in asthmatic children as compared with controls and children with chronic cough(P< 0.05, respectively). CD45RO-positive cell counts, EG2-positive cell counts and IL-5 levels in BALF correlated positively with one another (r= 0.785 3, P<0.001; r= 0.918 7, P<0.001; r= 0.759 1, P<0.001, respectively). \u0000 \u0000 \u0000Conclusions \u0000Eosinophils and epithelial cells in BALF were significantly increased in children with asthma in comparison with chronic cough, infantile wheezing and control subjects; some children with chronic cough also had increased eosinophil counts; neutrophil was the main significantly increased cell in BALF in the children with infantile wheezing. \u0000 \u0000 \u0000Key words: \u0000Child; Bronchoalveolar lavage fluid; Granulocytes; Asthma; Cough","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114437677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of NF-κB on endothelial damage mediated by inflammatory cytokines in Kawasaki disease","authors":"Jinrong Fu, Chengrong Li, Yufeng Zhou","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.02.104","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.02.104","url":null,"abstract":"Objective \u0000Kawasaki disease (KD) is the leading cause of acquired heart diseases in children. Its etiology is unknown. In recent years, data have shown that the dysfunction of T cell and monocytes/macrophages plays a central role in the development of vasculitis. Nuclear factor-κB(NF-κB) is positioned to integrate information from immune signaling pathway that can regulate the function of T cell and monocytes/macrophages. But the role of NF-κB in endothelial damage of KD is unknown. Therefore, the objective of the study was to further explore the effects of NF-κB on the endothelial damage in KD. \u0000 \u0000 \u0000Methods \u0000Human umbilical vein endothelial cells were cultured in vitro and the production of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay ( ELISA). The proportion of apoptotic cells in endothelial cells induced by the supernatants of peripheral blood mononuclear cells (PBMCs) was detected by AnnexinⅤ/PI double-staining. Electrophoretic mobility shift assay (EMSA) was used to detect the activity of NF-κB. \u0000 \u0000 \u0000Results \u0000The levels of TNF-α, IL-1β and IL-6 were markedly increased in patients and the proportion of apoptotic cells in endothelial cells induced by the cultured supernatants of PBMCs was markedly elevated (38.45±7.80)% compared to (2.87±0.76)% in the control subjects. The apoptosis induced by the supernatants of cultured PBMCs could be reversed, to some degree, through alone anti- TNF-α, IL-1β or IL-6 monoclonal antibody (McAb), or the combination together. The activity of NF-κB in the activated PBMCs in patients with KD was distinctly increased and SN50, the blockade of NF-κB, could significantly inhibit the production of TNF-α, IL-1β, IL-6 and the apoptosis of endothelial cells induced by the supernatants of cultured PBMC. \u0000 \u0000 \u0000Conclusion \u0000NF-κB, which can trigger the transcription of inflammatory cytokines such, as TNF-α, IL-1β, IL-6, plays an important role in endothelial damage of KD. \u0000 \u0000 \u0000Key words: \u0000Mucocutaneous lymph node synrome; NF-κB; Cytokine; Endothelium, rascular","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115629116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of hepatitis B virus DNA quantity in transmission between mothers and infants","authors":"Jian Lin, S. Liao, Guangzhou Guo, Wenyao Huang, Chun-ping Wang, Mingfang Zhou, Chun-chen Wu","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.02.108","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.02.108","url":null,"abstract":"Objective \u0000In order to investigate the influence of serum HBV-DNA quantity in pregnant women on HBV transmission between mothers and infants. \u0000 \u0000 \u0000Methods \u0000Serum HBV-DNA quantity was performed by fluorescence quantitative PCR in 69 paired mothers and infants. Different groups were divided according to the concentration of serum HBV-DNA. \u0000 \u0000 \u0000Results \u0000The average maternal serum HBV-DNA was(6.3±1.9) copies/ml, while the average infant serum HBV-DNA was (4.8±2.0) copies/ml. There was a positive correlation of serum HBV-DNA between paired mothers and infants (r=0.310, P<0.01). Of 69 infants, 45 were positive in HBV-DNA qualitative PCR test and (or) abnormal in HBV serological tests. The mother-infant transmission rate of HBV was 65%. The transmission rate increased when the serum HBV-DNA concentration increased in pregnant women. The cumulative calculation of different groups of maternal serum HBV-DNA showed that the mother-infant transmission rates were significantly different at the levels of 5.0, 6.0 and 7.0 copies/ml. The differences were 32%, 34% and 28%, respectively. In this study, 19 pregnant women were HBeAg positive, 17 were HBsAb positive. The HBV-DNA [(7.6±1.3) copies/ml] and mother-infant transmission rate (90%) in HBeAg positive group were significantly higher than those in HBeAg negative group [(5.8±1.9) copies/ml and 56.0% respectively]. HBV-DNA concentration [(5.3±1.6) copies/ml] and transmission rate (29%) in HBsAb positive group were significantly lower than those in HBsAb negative group [(6.6±1.9) copies/ml, and 76.9%]. Meanwhile HBeAg positive cases were mainly among those pregnant women with higher maternal serum HBV-DNA concentration, and HBsAb positive cases were mainly among the pregnant women with lower maternal serum HBV-DNA concentration. \u0000 \u0000 \u0000Conclusion \u0000HBV transmission rate was influenced by maternal serum HBV-DNA concentration. It seems that the maternal serum critical HBV-DNA levels may influence the mother-infant HBV transmission rate. \u0000 \u0000 \u0000Key words: \u0000Hepatitis B virus; DNA viruses; Disease transmission, vertical; Quantitative detection; Serum concentration interface","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129575138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Congenital atransferrinemia: a case report","authors":"G. Lin, L. Chen, Wei Wang, Yunzhu Li","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.02.112","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.02.112","url":null,"abstract":"Objective \u0000Congenital atransferrinemia (CAT) is an extremely rarely, recessively inherited disorder. To date, only eight cases have been reported in the published literatures of the world, and there is no report in China. The authors diagnosed a difficult and complicated case suffering from severe anemia for 3 years. \u0000 \u0000 \u0000Methods \u0000A thirteen-year-old boy was transferred to the Pediatric Department, Ruijin Hospital for recurrent anemia with significant hepatosplenomegaly three years. The child had required transfusion of whole blood and iron many times, but there was no benefit to the anemia. The child denied a family history of anemia, and his parents are normal. Diagnosis was confirmed according to the symptoms, signs, special laboratory examinations (liver biopsy, bone marrow biopsy, serum TRF, etc.) and the previously published papers of other authors. \u0000 \u0000 \u0000Results \u0000The patient manifested as severe anemia, hepatomegaly and splenectasis. The beta-globulin fraction was 5% (normal value: 7%~13%) on serum protein electrophoresis, and assays for TRF showed 0.0147g/L (normal value: 2.52~4.29g/L). The liver biopsy revealed the significant deposition of hemosiderin within the hepatocytes and kupffer cells. The parent of the boy showed a low level of TRF. His father′s TRF was 1.29g/L, and his mother′s TRF was 1.4 g/L. CAT is an autosomal recessive disease. \u0000 \u0000 \u0000Conclusion \u0000The diagnosis of congenital atransferrinemia was established, which remind that doctors must pay a great attention to this rare disease. \u0000 \u0000 \u0000Key words: \u0000Anemia; Transferrin; Hemosiderosis","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131882995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of WT1 gene expression in peripheral blood cells and bone marrow cells of childhood leukemia","authors":"Xiao-ling Bai, H. Zhao, Hui Yao, Nan Zheng, Bo Xu, Nianzheng Sun, G. Jiang, T. Tang","doi":"10.3760/CMA.J.ISSN.0578-1310.2002.02.106","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2002.02.106","url":null,"abstract":"Objective \u0000To set up a simple, quick and accurate method for monitoring minimal residual disease (MRD) of leukemia, the clinical significance of WT1 gene expression in peripheral blood (PB) cells was compared with that in bone marrow (BM) cells from children with the acute leukemia (AL). \u0000 \u0000 \u0000Methods \u0000Thirty-four children with AL were selected in this study, including 23 acute lymphoblastic leukemia (ALL) and 11 acute myeloid leukemia (AML). Normal PB cells from 8 healthy children and BM cells from 8 non-hematological disease patients were used as the negative control and K562 cells as the positive control. Relative levels of WT1 gene expression in PB and BM mononuclear cells from 34 AL were dynamically detected by using the nested reverse transcriptase-polymerase chain reaction (RT-PCR), and analyzed quantitatively with GQS-960 image processing system software. The WT1/β-actin ratio was calculated. \u0000 \u0000 \u0000Results \u0000(1) WT1 gene expression showed 0.784±0.311 in BM cells of 27 patients with recently diagnosis, and 0.734±0.295 in PB cells of 26 cases. There was no difference of the WT1 gene expression(P>0.05). (2) Twenty-six out of 32 cases with AL achieved complete remission (CR) after chemotherapy, including 20 cases with WT1 positive expression in BM and PB, 6 cases with WT1 negative expression. The CR rate showed no difference. (3) Relative levels of WT1 gene expression in PB and BM markedly decreased after CR, 12 of 20 cases with WT1+ in BM changed to negative expression of WT1 gene. Eight cases still lasted positive (0.376±0.162 ). Fifteen of 20 cases with WT1+ in PB changed to negative expression, 8 cases still lasted positive (0.229±0.105 ). Six NR patients showed continued positive expression of WT1 gene (0.912±0.241 ). (4) Eighteen patients with WT1+ at the diagnosis were followed up from 6 to 24 months. Five out of 8 cases of WT1+ in BM relapsed, 3 out of 10 cases WT1- relapsed, which showed no market difference. While 5 cases with WT1+ in PB were all relapse, 3 out of 13 cases with WT1- relapsed, which showed significant difference (P=0.006 5). \u0000 \u0000 \u0000Conclusion \u0000The dynamic analysis of WT1 gene expression in children with AL could be used to monitor MRD and predict early relapse. WT1 gene expression detected in PB cells of MRD seems to be more specific and convenient than that detected in BM cells. \u0000 \u0000 \u0000Key words: \u0000Leukemia; Neoplasm residual; Oncogenes; Gene expression; Polymerase chain reaction","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123559575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"~(13)C-urea breath test in the diagnosis of Helicobacter pylori infection in children","authors":"Liying Jia, S. Dong","doi":"10.3760/CMA.J.ISSN.0578-1310.2001.12.114","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2001.12.114","url":null,"abstract":"Objective \u0000To evaluate the reliability of 13 C-urea breath test (13C-UBT) in the diagnosis of Helicobacter Pylori (HP) infection in children by using gastroscopy and serum HP antibody study. \u0000 \u0000 \u0000Methods \u0000Totally 589 children with repeatedly occurred epigastric or periumbilical pain were studied from October 1997 to December 1999 in outpatients clinic of gastroenterology of our hospital.The patients′ ages ranged from 4 to 14 years, the average age was 9 years. Of the 589 patients, 435 were examined with 13 C-UBT. After overnight fasting, in the morning each child received a cup of oat meal to delay gastric emptying and 50 mg of 13 C-urea was administrated, and before and 30 minute after the dose, expired gases were collected for analysis with isotope ratio mess spectrometry. The result were expressed as DOB (Delta over baseline). Two hundred and forty-seven and 188 cases have stopped using antibiotics 2 and 4 weeks before examination, respectively. At the same time 41 of 435 cases with serious symptoms were examined with gastroscopy and antral biopsies were obtained for Warthin-Starry staining (W-S stain) and rapid urease test (RUT). Diagnosis of HP infection was made when both examinations were positive. The rate of consistency betwenn 13 C-UBT and gastroscopy, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated. At the same time HP-IgG antibody was examined in 300 of 589 cases, while 13 C-UBT was performed in 146 of 300 cases. The consistency between 13 C-UBT and serum HP-IgG antibody was also calculated. \u0000 \u0000 \u0000Results \u0000Positive results of 13 C-UBT were seen in 27.58% of the patients, the rate of infection rose with increase of patients′ age, which was remarkable after 7 years of age. The rate of infection increased very fast during childhood. The patterns of infection were similar to that in the other developing countries. In patients who had not received antibiotics for 2 weeks and 4 weeks, the rates of HP infection were 27.12% and 28.17%, respectively. The positive rate of serum HP-IgG antibody was 56.7%. The HP like microorganism was seen in histologic study after gastroscopy in 39% of the cases. The consistency rate between gastroscopy and 13C-UBT was 90.24%. The sensitivity, specificity, PPV and NPV were 75%, 100%, 100%, and 86.2%, respectively. The rate of consistency between 13 C-BUT and serum HP-IgG antibody were 63.1%. \u0000 \u0000 \u0000Conclusions \u000013 C-UBT is a method with higher sensitivity and high specificity. As a noninvasive method, it is reliable for detection of HP infection in children. There was no significant difference in positive rate of 13 C-UBT between inpatients without antibiotics use for 2 weeks and 4 weeks before examination. \u0000 \u0000 \u0000Key words: \u0000Helicobacter pylori; 13C-urea breath test; Urease","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126067256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}