Pub Date : 2000-01-16DOI: 10.3760/CMA.J.ISSN.0578-1310.2000.01.115
J. Niu, Lüzhou Guo
Objective To study the role of insulin-like growth factor type I receptors (IGF-IR) in the modulation of cord blood T lymphocyte functions and its clinical significance. Methods The antisense oligonucleotide was used to inhibit the expression of IGF-IR mRNA of cord blood lymphocyte. By using the reverse transcriptase polymerase chain reaction and3 H thymidine uptake technique, the mRNA expressions of IGF-IR, IL-2, IL-4, IFN-γ as well as the proliferation of lymphocyte were detected. Results A 50% reduction in IGF-IR mRNA following exposure to antisense oligonucleotide was observed. The proliferation of T-cell to mitogens was significantly reduced about 50%. The mRNA levels of IL-2, IL-4 and IFN-γ were also markedly decreased after using IGF-IR antisense oligon ucleotide. Compared with the expore to PHA, the mRNA levels of IL-2 and IFN-γdecreased by 30% ~50%, and IFN-γ decreased 20% ~ 30%. Conclusion IGF-IR was involved in the process of cord blood lymphocyte proliferation and the production of cytokines. It might play an important role in maintaining cell immune functions and TH1/TH2 relative balance in infants. Key words: Receptors, insulin-like growth factor I; Oligonucleotides, antisense; Fetal blood; Lymphocytes; Cytokines
{"title":"Effects of insulin-like growth factor type I receptors on cord blood T lymphocyte functions and its clinical significance","authors":"J. Niu, Lüzhou Guo","doi":"10.3760/CMA.J.ISSN.0578-1310.2000.01.115","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2000.01.115","url":null,"abstract":"Objective \u0000To study the role of insulin-like growth factor type I receptors (IGF-IR) in the modulation of cord blood T lymphocyte functions and its clinical significance. \u0000 \u0000 \u0000Methods \u0000The antisense oligonucleotide was used to inhibit the expression of IGF-IR mRNA of cord blood lymphocyte. By using the reverse transcriptase polymerase chain reaction and3 H thymidine uptake technique, the mRNA expressions of IGF-IR, IL-2, IL-4, IFN-γ as well as the proliferation of lymphocyte were detected. \u0000 \u0000 \u0000Results \u0000A 50% reduction in IGF-IR mRNA following exposure to antisense oligonucleotide was observed. The proliferation of T-cell to mitogens was significantly reduced about 50%. The mRNA levels of IL-2, IL-4 and IFN-γ were also markedly decreased after using IGF-IR antisense oligon ucleotide. Compared with the expore to PHA, the mRNA levels of IL-2 and IFN-γdecreased by 30% ~50%, and IFN-γ decreased 20% ~ 30%. \u0000 \u0000 \u0000Conclusion \u0000IGF-IR was involved in the process of cord blood lymphocyte proliferation and the production of cytokines. It might play an important role in maintaining cell immune functions and TH1/TH2 relative balance in infants. \u0000 \u0000 \u0000Key words: \u0000Receptors, insulin-like growth factor I; Oligonucleotides, antisense; Fetal blood; Lymphocytes; Cytokines","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128375028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-01-16DOI: 10.3760/CMA.J.ISSN.0578-1310.2000.01.109
Zhang Dongxia
Objective To explore the relationship between the red blood cell (RBC) immunoadhensive function and epilepsy seizure. Methods The rosette rate of RBC C3b receptor and rosette rate of RBC IC were measured by using yeast rosette method in 70 children with epilepsy and 30 healthy children (control group). Results The levels of the rosette rate of RBC C3b receptor were lower in epilepsy group than in control group ( t = -9.01, P 0.01); the levels of rosette rate of RBC IC were higher in epilepsy group than in control group ( t = 8 85, P 0 01). The levels of rosette rate of RBC C3b receptor were higher in patients taking antiepileptic drugs than patients without taking antiepileptic drugs ( t = 2 75, P 0 05); while rosette rate of RBC IC had no significant difference between the patients with and without antiepileptic drugs ( t = -1 80, P 0 05). There were no differences for the above indexes among the different types of epileptic syndromes ( F RBC C3b =0 081, F RBC IC =0 31, P 0 05). Conclusions RBC immunoadhensive function showed significant decrease in child patients with epilepsy; RBC immunoadhensive function had no significant difference in various types of epileptic syndromes; the red cell immunoadhensive functions were improved by controlling epileptic seizure.
目的探讨红细胞免疫粘附功能与癫痫发作的关系。方法采用酵母花环法测定70例癫痫患儿和30例健康儿童(对照组)的红细胞C3b受体花环率和红细胞IC花环率。结果癫痫组红细胞C3b受体玫瑰花结率低于对照组(t = -9.01, p0.01);癫痫组红细胞IC玫瑰花结率高于对照组(t = 885, P < 0.01)。服用抗癫痫药物的患者红细胞C3b受体玫瑰花结率高于未服用抗癫痫药物的患者(t = 2.75, P < 0.05);而使用抗癫痫药物组与未使用抗癫痫药物组RBC IC玫瑰花结率差异无统计学意义(t = -1 80, P < 0.05)。不同类型癫痫综合征患者上述指标比较差异无统计学意义(F RBC C3b =0 081, F RBC IC =0 31, P < 0.05)。结论儿童癫痫患者红细胞免疫粘附功能明显下降;红细胞免疫粘附功能在各类型癫痫综合征中无显著差异;通过控制癫痫发作，红细胞免疫粘附功能得到改善。
{"title":"The study of red blood cell immune function in children with epilepsy","authors":"Zhang Dongxia","doi":"10.3760/CMA.J.ISSN.0578-1310.2000.01.109","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0578-1310.2000.01.109","url":null,"abstract":"Objective To explore the relationship between the red blood cell (RBC) immunoadhensive function and epilepsy seizure. Methods The rosette rate of RBC C3b receptor and rosette rate of RBC IC were measured by using yeast rosette method in 70 children with epilepsy and 30 healthy children (control group). Results The levels of the rosette rate of RBC C3b receptor were lower in epilepsy group than in control group ( t = -9.01, P 0.01); the levels of rosette rate of RBC IC were higher in epilepsy group than in control group ( t = 8 85, P 0 01). The levels of rosette rate of RBC C3b receptor were higher in patients taking antiepileptic drugs than patients without taking antiepileptic drugs ( t = 2 75, P 0 05); while rosette rate of RBC IC had no significant difference between the patients with and without antiepileptic drugs ( t = -1 80, P 0 05). There were no differences for the above indexes among the different types of epileptic syndromes ( F RBC C3b =0 081, F RBC IC =0 31, P 0 05). Conclusions RBC immunoadhensive function showed significant decrease in child patients with epilepsy; RBC immunoadhensive function had no significant difference in various types of epileptic syndromes; the red cell immunoadhensive functions were improved by controlling epileptic seizure.","PeriodicalId":416525,"journal":{"name":"Chinexe Journal of Pediatrics","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124156759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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