Pub Date : 2023-12-01DOI: 10.5398/tasj.2023.46.4.471
N. Oanh, C. T. T. Thu, N. V. Don, J. Hornick
{"title":"Growth Performance and Pork Quality of Finishing Pigs Fed Diet Supplemented with Sacha Inchi Oil and Herbal Plants","authors":"N. Oanh, C. T. T. Thu, N. V. Don, J. Hornick","doi":"10.5398/tasj.2023.46.4.471","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.4.471","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138609919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.5398/tasj.2023.46.4.389
H. Hartati, W. P. B. Putra
{"title":"Genome-Wide Association Study for Body Weight and Carcass Weight in Sumba Ongole Bulls (Bos indicus)","authors":"H. Hartati, W. P. B. Putra","doi":"10.5398/tasj.2023.46.4.389","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.4.389","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138626771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.5398/tasj.2023.46.4.509
R. W. Ouédraogo, K. Tindano, F. G. Traoré, S. A. R. Tapsoba, M. Sanou, H. Tamboura, B. Bayala, A. Traoré
{"title":"Local Chickens Farming System Characteristics and Their Genetic Resources Management in Seno Province of Burkina Faso","authors":"R. W. Ouédraogo, K. Tindano, F. G. Traoré, S. A. R. Tapsoba, M. Sanou, H. Tamboura, B. Bayala, A. Traoré","doi":"10.5398/tasj.2023.46.4.509","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.4.509","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138622483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.5398/tasj.2023.46.3.287
D. Lestari, S. Murtini, N. Ulupi, A. Gunawan, C. Sumantri
The CD4 gene plays an important role in the immune process by producing CD4 molecules that aid in producing antibodies. IPB-D2 chickens are selected from IPB-D2 chickens based on IgY concentration and ND antibody titer. This study aimed to analyze the polymorphism of the CD4 gene, unravel the mRNA expression of CD4 gene in IPB-D2 chicken related to the immunocompetence index, and detect the CD4 gene pathway. The total samples used were 100 IPB-D2 G2 chickens aged 21 weeks. Blood samples were collected for ELISA test, HI test, sequencing test, and seca tonsil tissue for relative mRNA expression. Polymorphism and association data were analyzed using MEGAX, FinchTV, SNPstat, and DNAsp. The relative mRNA expression analysis was conducted using qRT-PCR. The pathway analysis of the CD4 gene was performed using the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analysis. The result showed there were 4 SNPs of the CD4 gene in IPB-D2 chicken, i.e., g.7526 C>T, g.7825 C>T, g.8100 C>A, and g.8157 T>A. All CD4 SNPSs showed no association with IgY concentration and ND antibody titers. Relative mRNA expression shows that IPB-D2 chickens with high ND antibody titers have a higher level of expression when compared to IPB-D2 chickens with low ND antibody titers. Furthermore, pathway analysis showed the CD4 gene involved in the T cell receptor (TcR) signaling process. This study concludes that the CD4 gene is polymorphic and involved in the T cell receptor signaling process. This study demonstrated that polymorphisms of the CD4 gene in IPB-D2 chicken might not contribute to the IgY concentration and ND antibody titer but can serve as a reference in the study of CD4 genes in the other chicken breeds related to the other immunocompetence index.
{"title":"Association and Expression of Cluster of Differentiation 4 (CD4) Gene in IPB-D2 Chicken Related to Immunocompetence Index","authors":"D. Lestari, S. Murtini, N. Ulupi, A. Gunawan, C. Sumantri","doi":"10.5398/tasj.2023.46.3.287","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.3.287","url":null,"abstract":"The CD4 gene plays an important role in the immune process by producing CD4 molecules that aid in producing antibodies. IPB-D2 chickens are selected from IPB-D2 chickens based on IgY concentration and ND antibody titer. This study aimed to analyze the polymorphism of the CD4 gene, unravel the mRNA expression of CD4 gene in IPB-D2 chicken related to the immunocompetence index, and detect the CD4 gene pathway. The total samples used were 100 IPB-D2 G2 chickens aged 21 weeks. Blood samples were collected for ELISA test, HI test, sequencing test, and seca tonsil tissue for relative mRNA expression. Polymorphism and association data were analyzed using MEGAX, FinchTV, SNPstat, and DNAsp. The relative mRNA expression analysis was conducted using qRT-PCR. The pathway analysis of the CD4 gene was performed using the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analysis. The result showed there were 4 SNPs of the CD4 gene in IPB-D2 chicken, i.e., g.7526 C>T, g.7825 C>T, g.8100 C>A, and g.8157 T>A. All CD4 SNPSs showed no association with IgY concentration and ND antibody titers. Relative mRNA expression shows that IPB-D2 chickens with high ND antibody titers have a higher level of expression when compared to IPB-D2 chickens with low ND antibody titers. Furthermore, pathway analysis showed the CD4 gene involved in the T cell receptor (TcR) signaling process. This study concludes that the CD4 gene is polymorphic and involved in the T cell receptor signaling process. This study demonstrated that polymorphisms of the CD4 gene in IPB-D2 chicken might not contribute to the IgY concentration and ND antibody titer but can serve as a reference in the study of CD4 genes in the other chicken breeds related to the other immunocompetence index.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44686482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.5398/tasj.2023.46.3.313
B. Ma'rifah, I. Agusetyaningsih, T. Sarjana, S. Kismiati, S. Sugiharto
{"title":"Effect of Moringa oleifera Leaves Extract, Whey Protein, and Their Combination on Growth, Carcass and Meat Quality of Broiler Chickens","authors":"B. Ma'rifah, I. Agusetyaningsih, T. Sarjana, S. Kismiati, S. Sugiharto","doi":"10.5398/tasj.2023.46.3.313","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.3.313","url":null,"abstract":"","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48037957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.5398/tasj.2023.46.3.354
P. Promtan, J. Panatuk, W. Kongbuntad, D. Amornlerdpison, Y. Nanta, N. Pripwai, W. Thaworn, W. Pattanawong
This research was conducted to investigate the growth performance of black-boned chicken embryonic stem (ES) cells for the future development of cultured meat. Black-boned chicken ES cells were isolated and cultured from fertilized eggs. The treatments applied were: fetal bovine serum (FBS) (T1), commercial chicken serum (SCK) (T2), Pradu Hang Dam chicken serum (PDC) (T3), and black-boned chicken serum (BBC) (T4). Black-boned chicken ES cells were cultured at 37.0 °C in a humidified environment of 5% CO 2 , for 10 days. The growth of black-boned chicken ES cells concentration was measured by the absorbance at 450 nm. A haemocytometer was used to count the number of black-boned chicken ES cells. Comparing the protein content of cultured meat and chicken meat was collected for combustion and proximate analysis. All collected data were analyzed using ANOVA in a completely randomized design. T4 tended to have a higher number and growth rate than the other groups, followed by T3, T2, and T1, respectively. When counted ES cells final, T4 had a significantly higher number and growth rate than the other groups (p<0.001). Comparing the protein content, it was found that cultured meat had significantly more protein than the other groups (p<0.001), and characteristics (alignment and cross-section of muscle fibers) were not different from chicken cells. Finally, the cultivation of black-boned chicken ES cells necessitates the use of a medium containing black-boned chicken serum in the growth and development of black-boned Chicken ES cells for culture meat.
本研究旨在研究乌骨鸡胚胎干细胞（ES）的生长性能，为培养肉的未来发展提供参考。从受精卵中分离和培养黑骨鸡ES细胞。所用的处理方法为：胎牛血清（FBS）（T1）、商品鸡血清（SCK）（T2）、Pradu Hang Dam鸡血清（PDC）（T3）和乌骨鸡血清（BBC）（T4）。将乌骨鸡ES细胞在37.0°C、5%CO2的湿润环境中培养10天。通过在450nm处的吸光度测量乌骨鸡ES细胞的生长浓度。使用血细胞仪对乌骨鸡ES细胞的数量进行计数。将培养肉和鸡肉的蛋白质含量进行比较，以进行燃烧和近似分析。在完全随机设计中，使用ANOVA对所有收集的数据进行分析。T4的数量和生长率往往高于其他组，其次分别是T3、T2和T1。当最终计数ES细胞时，T4的数量和生长速率显著高于其他组（p＜0.001）。比较蛋白质含量，发现培养的肉比其他组具有显著更多的蛋白质（p＜001），并且特征（肌肉纤维的排列和横截面）与鸡细胞没有差异。最后，培养乌骨鸡ES细胞需要使用含有乌骨鸡血清的培养基来生长和发育用于培养肉的乌骨鸡ES细胞。
{"title":"Growth and Development of Black-Boned Chicken Embryonic Stem Cells for Culture Meat using Different Serums as Medium","authors":"P. Promtan, J. Panatuk, W. Kongbuntad, D. Amornlerdpison, Y. Nanta, N. Pripwai, W. Thaworn, W. Pattanawong","doi":"10.5398/tasj.2023.46.3.354","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.3.354","url":null,"abstract":"This research was conducted to investigate the growth performance of black-boned chicken embryonic stem (ES) cells for the future development of cultured meat. Black-boned chicken ES cells were isolated and cultured from fertilized eggs. The treatments applied were: fetal bovine serum (FBS) (T1), commercial chicken serum (SCK) (T2), Pradu Hang Dam chicken serum (PDC) (T3), and black-boned chicken serum (BBC) (T4). Black-boned chicken ES cells were cultured at 37.0 °C in a humidified environment of 5% CO 2 , for 10 days. The growth of black-boned chicken ES cells concentration was measured by the absorbance at 450 nm. A haemocytometer was used to count the number of black-boned chicken ES cells. Comparing the protein content of cultured meat and chicken meat was collected for combustion and proximate analysis. All collected data were analyzed using ANOVA in a completely randomized design. T4 tended to have a higher number and growth rate than the other groups, followed by T3, T2, and T1, respectively. When counted ES cells final, T4 had a significantly higher number and growth rate than the other groups (p<0.001). Comparing the protein content, it was found that cultured meat had significantly more protein than the other groups (p<0.001), and characteristics (alignment and cross-section of muscle fibers) were not different from chicken cells. Finally, the cultivation of black-boned chicken ES cells necessitates the use of a medium containing black-boned chicken serum in the growth and development of black-boned Chicken ES cells for culture meat.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42200109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.5398/tasj.2023.46.3.347
H. Kim, K. W. Lee, B. G. Kim
The objective of the present work was to determine the optimal supplemental chromium (Cr) concentration for alleviating the detrimental effects of heat stress on the growth performance of broilers using the literature data. A total of 53 observations from 22 experiments that assessed the growth performance of broilers fed various doses of Cr under heat stress were collected. The control groups received no supplemental Cr, whereas supplemental Cr concentrations ranged from 200 µg/ kg to 2,000 µg/kg of diet. The sources of Cr were Cr chloride (n= 12), Cr-amino acid chelate (n= 14), and Cr picolinate (n= 27). The relative change (Δ %) of average daily gain (ADG) between broilers fed a Cr-supplemented diet and those fed a control diet was calculated. To compare ΔADG among Cr sources, the source was considered a fixed variable, while the experiment and the supplemental Cr concentration were considered random variables. The ΔADG was not different among the sources. Polynomial contrast analysis indicated that ΔADG increased quadratically (p<0.05) as the dietary Cr concentration increased. The optimum supplemental Cr concentration was estimated using a one-slope broken-line model with the fixed variable of supplemental Cr and a random variable of experiment based on the NLMIXED procedure of SAS. The optimum supplemental Cr concentration to maximize ΔADG in broilers under heat stress was 687 µg/kg (SE= 137, R 2 = 0.70, and p<0.001). Taken together, the optimum Cr supplemental concentration in broiler diets to alleviate the detrimental effects of heat stress on body weight gain is 687 µg/kg, regardless of the source of Cr.
{"title":"Optimal Supplemental Chromium Concentration for Alleviating Heat Stress in Broiler Chickens: A Meta-analysis","authors":"H. Kim, K. W. Lee, B. G. Kim","doi":"10.5398/tasj.2023.46.3.347","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.3.347","url":null,"abstract":"The objective of the present work was to determine the optimal supplemental chromium (Cr) concentration for alleviating the detrimental effects of heat stress on the growth performance of broilers using the literature data. A total of 53 observations from 22 experiments that assessed the growth performance of broilers fed various doses of Cr under heat stress were collected. The control groups received no supplemental Cr, whereas supplemental Cr concentrations ranged from 200 µg/ kg to 2,000 µg/kg of diet. The sources of Cr were Cr chloride (n= 12), Cr-amino acid chelate (n= 14), and Cr picolinate (n= 27). The relative change (Δ %) of average daily gain (ADG) between broilers fed a Cr-supplemented diet and those fed a control diet was calculated. To compare ΔADG among Cr sources, the source was considered a fixed variable, while the experiment and the supplemental Cr concentration were considered random variables. The ΔADG was not different among the sources. Polynomial contrast analysis indicated that ΔADG increased quadratically (p<0.05) as the dietary Cr concentration increased. The optimum supplemental Cr concentration was estimated using a one-slope broken-line model with the fixed variable of supplemental Cr and a random variable of experiment based on the NLMIXED procedure of SAS. The optimum supplemental Cr concentration to maximize ΔADG in broilers under heat stress was 687 µg/kg (SE= 137, R 2 = 0.70, and p<0.001). Taken together, the optimum Cr supplemental concentration in broiler diets to alleviate the detrimental effects of heat stress on body weight gain is 687 µg/kg, regardless of the source of Cr.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48799421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.5398/tasj.2023.46.3.280
P. Silalahi, Y. C. Chen
Selection for reproductive components in various pig populations in the world has been successfully performed. This study aimed to estimate the genetic parameters and genetic trends of reproductive traits of Taiwan Duroc, Landrace, and Yorkshire pigs. Data were extracted from the Taiwan Swine Registry Database from 2009 to June 2018. The number of farrowing records used in this study was 6,504, 6,398, and 2,178 for Duroc, Landrace, and Yorkshire, respectively. Litter traits measured were the number of piglets born alive (NBA), number of piglets at 21 days of age (N21D), litter weight at birth (LWB), and litter weight at 21 days of age (LW21D). Variances estimates obtained from univariate analysis with a repeatability model were used to estimate heritability and repeatability. Heritability estimates were 0.058±0.017, 0.037±0.014, and 0.101±0.032 for NBA; 0.086±0.018, 0.102±0.019, and 0.151±0.035 for N21D; 0.036±0.011, 0.111±0.021, and 0.330±0.05 for LWB; 0.119±0.020, 0.168±0.023, and 0.237±0.045 for LW21D in the Duroc, Landrace, and Yorkshire breeds, respectively. The genetic trends of NBA were 0.005 piglets/year for the Duroc and 0.002/piglets for the Landrace. In Yorkshire, conversely, there was no significant genetic improvement of NBA, but there was a 0.011 kg/year improvement of LWB. Among all genetic trends, it was shown that genetic gain in Taiwan pig populations was very low. In addition, it also suggested that genomic selection could be used in the Taiwan pig breeding program to push the rate of genetic gain.
{"title":"Estimation of Genetic Parameters for Litter Traits in Taiwan Duroc, Landrace, and Yorkshire Pigs","authors":"P. Silalahi, Y. C. Chen","doi":"10.5398/tasj.2023.46.3.280","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.3.280","url":null,"abstract":"Selection for reproductive components in various pig populations in the world has been successfully performed. This study aimed to estimate the genetic parameters and genetic trends of reproductive traits of Taiwan Duroc, Landrace, and Yorkshire pigs. Data were extracted from the Taiwan Swine Registry Database from 2009 to June 2018. The number of farrowing records used in this study was 6,504, 6,398, and 2,178 for Duroc, Landrace, and Yorkshire, respectively. Litter traits measured were the number of piglets born alive (NBA), number of piglets at 21 days of age (N21D), litter weight at birth (LWB), and litter weight at 21 days of age (LW21D). Variances estimates obtained from univariate analysis with a repeatability model were used to estimate heritability and repeatability. Heritability estimates were 0.058±0.017, 0.037±0.014, and 0.101±0.032 for NBA; 0.086±0.018, 0.102±0.019, and 0.151±0.035 for N21D; 0.036±0.011, 0.111±0.021, and 0.330±0.05 for LWB; 0.119±0.020, 0.168±0.023, and 0.237±0.045 for LW21D in the Duroc, Landrace, and Yorkshire breeds, respectively. The genetic trends of NBA were 0.005 piglets/year for the Duroc and 0.002/piglets for the Landrace. In Yorkshire, conversely, there was no significant genetic improvement of NBA, but there was a 0.011 kg/year improvement of LWB. Among all genetic trends, it was shown that genetic gain in Taiwan pig populations was very low. In addition, it also suggested that genomic selection could be used in the Taiwan pig breeding program to push the rate of genetic gain.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46881626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.5398/tasj.2023.46.3.306
A. Baharun, Andika Fajar Setiawan, A. Rahmi, H. Iskandar, M. Gunawan, S. Anwar, T. Maulana, E. M. Kaiin, S. Said
Strategies to increase the population and productivity of beef cattle can be implemented through reproductive management, such as artificial insemination (AI) using frozen semen. This study aims to evaluate the characteristics of frozen semen of Limousin bulls of different ages, which can still be used for insemination programs up to 12 years of age. This study used frozen semen of Limousin bulls ages 3, 8, and 12 years produced in 2021 at the Artificial Insemination Center in Singosari, East Java Province, Indonesia. Five bulls from each different age group were used for replication. Computer-assisted sperm analysis was used to determine sperm motility, viability, and abnormalities using eosin–nigrosine staining. Plasma membrane integrity was analyzed using the hypoosmotic swelling test. The acrosomes’ integrity was evaluated using FITC-PNA-PI, protamine deficiency using chromomycin A3, and DNA fragmentation testing using the acridine orange fluorescent technique. The results showed that the parameters of sperm motility, viability, abnormality, plasma membrane integrity, and protamine deficiency showed no significant differences in all age groups. The kinematic parameters (straightness and beat cross frequency) of the 3-year-old group were significantly higher (p<0.05) compared with those of the other groups. Parameters of acrosome integrity showed a higher prevalence in the 3-year-old group compared with those of the other groups. Furthermore, the DNA fragmentation of the 12-year-old group was significantly higher (p<0.05) compared with that of the other groups. The research concludes that increasing the age of Limousin bulls can reduce acrosome integrity and DNA fragmentation.
{"title":"Frozen Semen Characteristics of Limousin Bull at Different Ages","authors":"A. Baharun, Andika Fajar Setiawan, A. Rahmi, H. Iskandar, M. Gunawan, S. Anwar, T. Maulana, E. M. Kaiin, S. Said","doi":"10.5398/tasj.2023.46.3.306","DOIUrl":"https://doi.org/10.5398/tasj.2023.46.3.306","url":null,"abstract":"Strategies to increase the population and productivity of beef cattle can be implemented through reproductive management, such as artificial insemination (AI) using frozen semen. This study aims to evaluate the characteristics of frozen semen of Limousin bulls of different ages, which can still be used for insemination programs up to 12 years of age. This study used frozen semen of Limousin bulls ages 3, 8, and 12 years produced in 2021 at the Artificial Insemination Center in Singosari, East Java Province, Indonesia. Five bulls from each different age group were used for replication. Computer-assisted sperm analysis was used to determine sperm motility, viability, and abnormalities using eosin–nigrosine staining. Plasma membrane integrity was analyzed using the hypoosmotic swelling test. The acrosomes’ integrity was evaluated using FITC-PNA-PI, protamine deficiency using chromomycin A3, and DNA fragmentation testing using the acridine orange fluorescent technique. The results showed that the parameters of sperm motility, viability, abnormality, plasma membrane integrity, and protamine deficiency showed no significant differences in all age groups. The kinematic parameters (straightness and beat cross frequency) of the 3-year-old group were significantly higher (p<0.05) compared with those of the other groups. Parameters of acrosome integrity showed a higher prevalence in the 3-year-old group compared with those of the other groups. Furthermore, the DNA fragmentation of the 12-year-old group was significantly higher (p<0.05) compared with that of the other groups. The research concludes that increasing the age of Limousin bulls can reduce acrosome integrity and DNA fragmentation.","PeriodicalId":42234,"journal":{"name":"Tropical Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47096934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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