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Genetic Diversity of Various Goat Breeds in East Java Based on DNA Microsatellite Markers 基于DNA微卫星标记的东爪哇不同山羊品种遗传多样性研究
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.247
T. Susilorini, D. Wulandari, A. Furqon, W. A. Septian, F. Saputra, S. Suyadi
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引用次数: 0
Factors Associated with the Odds of Pregnancy for Dairy Cattle after Treatment of Ovarian Disorders in Northern Vietnam 越南北部奶牛卵巢疾病治疗后怀孕几率的相关因素
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.277
S. Long, N. C. Toan, P. V. Gioi, P. T. Hang
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引用次数: 0
The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents 不同精液稀释剂对利木赞公牛冷冻精液质量的影响
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.284
A. A. Arif, T. Maulana, E. M. Kaiin, B. Purwantara, R. Arifiantini
Diluents are substances added to the semen to increase the semen volume, reduce sperm density, and maintain sperm viability. This study aimed to compare the effects of various semen diluents on the quality of frozen semen of Limousin bull. Three mature Limousin bulls were used as semen sources. The semen was collected using an artificial vagina and then evaluated for its quality. Sperms with motility less than 70% and abnormality greater than 20% were excluded from this study. After semen evaluation, each ejaculate was individually divided into four equal tubes and diluted with skimmed milk-egg yolk (SMEY), Tris–egg yolk (TEY), Tris–egg yolk–skimmed milk (TEYSM), or Andromed ® . Diluted semen was loaded into a mini straw and then equilibrated at 5 °C for 4 h. Following equilibra-tion, the straws were frozen. The percentages of sperm motility, viability, intact membrane, and sperm DNA integrity were evaluated after freezing. Furthermore, malondialdehyde (MDA) and aspartate aminotransferase (AST) enzyme concentrations were assessed after thawing. The sperm motility with TEY, TEYSM, and Andromed ® did not differ significantly but was higher than with SMEY. Sperm viability, membrane intactness, and sperm DNA integrity did not differ among treatments. By contrast, the MDA levels and concentration of AST in the Andromed ® diluent semen showed the lowest values compared with SMEY semen (p<0.05) but did not differ from those of TEY and TEYSM semen. To conclude, TEY, TEYSM, and Andromed ® diluents performed equally well, and although they showed better results than SMEY, sperm diluted in SMEY are still suitable for artificial insemination.
稀释剂是添加到精液中以增加精液体积、降低精子密度和保持精子活力的物质。本研究旨在比较不同精液稀释液对利穆赞公牛冷冻精液质量的影响。三头成熟的利穆赞公牛被用作精液来源。使用人工阴道采集精液,然后对其质量进行评估。精子活力低于70%和异常大于20%的精子被排除在本研究之外。精液评估后,将每份精液单独分为四个相等的管,并用脱脂牛奶蛋黄(SMEY)、Tris-蛋黄(TEY)、Tris--蛋黄-脱脂牛奶(TEYSM)或Andromed®稀释。将稀释的精液装入迷你吸管中,然后在5°C下平衡4小时。平衡后,将吸管冷冻。冷冻后评估精子活力、活力、完整膜和精子DNA完整性的百分比。此外,解冻后评估丙二醛(MDA)和天冬氨酸氨基转移酶(AST)的浓度。TEY、TEYSM和Andromed®的精子活力没有显著差异,但高于SMEY。精子活力、膜完整性和精子DNA完整性在不同处理之间没有差异。相反,与SMEY精液相比,Andromed®稀释精液中的MDA水平和AST浓度显示出最低值(p<0.05),但与TEY和TEYSM精液没有差异。总之,TEY、TEYSM和Andromed®稀释剂表现同样良好,尽管它们显示出比SMEY更好的结果,但在SMEY中稀释的精子仍然适合人工授精。
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引用次数: 3
The Influence of a Tropical Climate with a Long Dry Season on Goat Semen Quality and Seminal Fluid Change in the Kanchanaburi Province, Thailand 长旱季的热带气候对泰国北碧府山羊精液质量和精液变化的影响
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.291
S. Kimsakulvech, C. Nakthong, Y. Lanamtiang, T. Wongtawan
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引用次数: 0
Protodioscin Content, Degradation Kinetics, and In Vitro Digestibility of Marandu Palisadegrass Hay as were Affected by Cutting Interval of the Canopy 冠层刈割间隔对马兰度牧草原薯蓣皂苷含量、降解动力学及体外消化率的影响
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.299
F. J. O. Coca, E. N. O. Gomes, L. Junges, L. C. V. Itavo, L. M. Nonato, F. K. Gomes, C. Ítavo, G. S. Difante, A. M. Dias
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引用次数: 1
The Color Fastness and Quality of Eco-Printed Leather with Different Types of Mordant in Natural Dyes from Mangrove Extract (Rhizophora mucronata) 红树林提取物天然染料中不同变质剂对生态印花革的色牢度和质量的影响
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.368
W. Pancapalaga, E. Ishartati, T. Ambarwati
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引用次数: 0
Selenium Application in Improving Chicory (Cichorium intybus) Productivity and Quality 硒在提高菊苣产量和品质中的应用
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.337
N. Umami, E. R. Rahayu, B. Suhartanto, A. Agus, Md. Mahmudur Rahman
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引用次数: 0
Production Potential and Quality of Panicum maximum Cultivars Established in a Semi-Arid Environment 半干旱条件下大穗品种的生产潜力和品质
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.308
M. Pereira, G. Difante, L. C. V. Itavo, J. G. Rodrigues, A. Gurgel, A. M. Dias, C. Ítavo, E. Véras, A. Costa, G. O. D. A. Monteiro
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引用次数: 3
A Novel SNPs of KISS1 Gene Strongly Associated with Litter Size in Indonesian Goat Breeds 印尼山羊KISS1基因的一个新SNPs与产仔数强相关
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-09-01 DOI: 10.5398/tasj.2022.45.3.255
A. Febriana, S. Sutopo, E. Kurnianto, W. Widiyanto
Kisspeptin is a protein encoded by the KISS1 gene which behaves as a key role by stimulating gonadotropin releasing hormone (GnRH) neuron activity directly in the reproductive axis. The objective of the present study was to determine the genetic diversity within intron 1 of KISS1 gene and to verify their association with fecundity traits which can be devoted as a marker assisted selection (MAS) for breeding selection. This study was established on three Indonesian native goat populations (Kacang, Kejobong, and Senduro). The PCR products were then sequenced in both directions. The DNA sequencing alignment resulted fifteen variants (one indel and fourteen SNPs), with SNP1, SNP2, SNP3, and SNP9 being novel single nucleotide polymorphisms (SNPs) of KISS1 gene intron 1. Genotype, haplotype, and parity were significantly associated with litter size. SNP8, SNP9, and SNP10 were correlated with overall means of litter size (LS) and LS at the first and the third parities (p<0.05). Additionally, novel SNP9 were in remarkably strong linkage disequilibrium with SNP8 and SNP10 (D’=1.00; r≥0.58; χ 2 ≥13.38), and allele A had putative binding sites for the NFIC which plays an important role in activating the expression of KISS1 gene. The H2 haplotype (CATAGCGCAACGCT) was found to have the highest litter size (p<0.0001). CC genotype at SNP8, AA genotype at novel SNP9, GA genotype at SNP10, and H2 haplotype were the excellent genotypes and haplotype that associated with the superior LS (p<0.05). Therefore, this result led to presume that these three SNPs and H2 haplotype can be acknowledged as prominent genetic markers for goat-breeding selection.
Kisspeptin是一种由KISS1基因编码的蛋白质,其通过直接刺激生殖轴中的促性腺激素释放激素(GnRH)神经元活性发挥关键作用。本研究的目的是确定KISS1基因内含子1内的遗传多样性,并验证它们与繁殖力性状的相关性,这可作为育种选择的标记辅助选择(MAS)。这项研究建立在三个印尼本地山羊种群(卡康、克约邦和森杜罗)上。然后对PCR产物进行双向测序。DNA测序比对产生了15个变体(1个indel和14个SNPs),其中SNP1、SNP2、SNP3和SNP9是KISS1基因内含子1的新单核苷酸多态性(SNPs)。基因型、单倍型和产次与产仔数显著相关。SNP8、SNP9和SNP10与第一和第三胎的总产仔数(LS)和LS相关(p<0.05)。此外,新的SNP9与SNP8和SNP10具有显著的强连锁不平衡(D’=1.00;r≥0.58;χ2≥13.38),等位基因A具有NFIC的假定结合位点,NFIC在激活KISS1基因表达中起着重要作用。H2单倍型(CATGCGCAACGCT)具有最高的产仔数(p<0.0001)。SNP8处的CC基因型、新SNP9处的AA基因型、SNP10处的GA基因型和H2单倍型是与优越LS相关的优秀基因型和单倍型。因此,这一结果使我们推测这三个SNPs和H2单倍型可以被认为是山羊育种选择的重要遗传标记。
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引用次数: 2
Mitigation of Polycyclic Aromatic Hydrocarbons Formation in Goat Satay by Shallots Juices Marination 沙葱汁腌制对山羊沙中多环芳烃形成的抑制作用
IF 0.8 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-06-01 DOI: 10.5398/tasj.2022.45.2.227
E. Saputro, L. Radiati, W. Warsito, D. Rosyidi
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引用次数: 2
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