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Established the Environmental Monitoring Program Indicators to Prevent Disease Infection and Promote Sustainable Development 建立环境监测项目指标,预防疾病感染,促进可持续发展
Pub Date : 2018-10-05 DOI: 10.31031/cjmi.2018.02.000534
Y. Shih
Marine aquaculture may also cause environmental degradation from feces, uneaten feed and use of chemicals if the local carrying capacity is exceeded [1]. The most important benthic impact relates to the formation of anoxic sediments [2-4] which can be easily monitored. Both sediment condition and waste accumulation are good indicators for detecting the environmental condition at aquaculture sites. Beveridge [5] revealed that environmental deterioration because of high organic matter concentrations in the sediments may affect the health of farmed fish and hence profitability. Waste and waste-makes quality management for marine aquaculture in other countries has not only focused on the release limitations of organic matter, and nutrients [6-8] but also on chemicals [9] used on the farms regardless wherever into estuaries or nearshore coastal waters [10,11]. This investigation uses an environmental monitoring program to understand and assess the environmental impact of marine farming.
如果超过当地的承载能力,海洋养殖还可能导致粪便、未食用的饲料和化学品的使用等环境退化[1]。最重要的底栖生物影响与缺氧沉积物的形成有关[2-4],这很容易监测。沉积物状况和废弃物积累都是检测养殖场环境状况的良好指标。贝弗里奇[5]发现,沉积物中有机物浓度高导致环境恶化,可能影响养殖鱼类的健康,从而影响盈利能力。其他国家对海洋水产养殖的废物和废物制造质量管理不仅关注有机物和营养物质的释放限制[6-8],而且关注养殖场使用的化学品[9],无论它们是否进入河口或近岸沿海水域[10,11]。这项调查使用环境监测程序来了解和评估海洋养殖对环境的影响。
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引用次数: 0
Genetic Resistance to Infectious Diseases in the Era of Personalized Medicine 个体化医疗时代对传染病的遗传抗性
Pub Date : 2018-09-25 DOI: 10.31031/CJMI.2018.02.000536
A. Alimov
Individual particularities of resistance to pathogens have been the focus of clinicians and researchers for centuries. Several theories and concepts were formulated to explain this phenomenon. They include the immunological theory of infectious diseases, the genetic theory of infectious diseases, the concept of latent and asymptomatic infection, monogenic and polygenic type of inheritance of resistance to pathogens and a number of others [1]. Nowadays, most of these concepts are reflected in the modern theory called “A Unified Genetic theory of infection disease”. The theory assumes that at an early age susceptibility to pathogenic microorganisms is caused by inherited variants of genes, while in adults features of a disease course depend on the total action of several genetic loci. In elderly people, a special role in the initiation and development of infectious disease plays somatic mutations [2]. In this regard, recently developed methodological approaches to wide genome analysis allow us to hope for a significant expansion of our understanding of genetic resistance to infectious diseases at both individual and population levels.
几个世纪以来,个体对病原体的耐药性一直是临床医生和研究人员关注的焦点。人们提出了一些理论和概念来解释这种现象。它们包括传染病的免疫学理论,传染病的遗传学理论,潜伏和无症状感染的概念,单基因和多基因遗传类型对病原体的抗性以及其他一些理论。如今,这些概念大多反映在现代的“传染病统一遗传理论”中。该理论认为,在早期,对致病微生物的易感性是由基因的遗传变异引起的,而在成人中,疾病病程的特征取决于几个遗传位点的总作用。在老年人中,在传染病的发生和发展中起特殊作用的是体细胞突变[2]。在这方面,最近发展的广泛基因组分析的方法学方法使我们能够希望在个体和群体水平上大大扩展我们对传染病的遗传抗性的理解。
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引用次数: 0
The Evaluation of the Speed-Oligo® Mycobacteria Assay for Identification of Mycobacterium spp. from Smear Positive and Negative Sputum Samples Speed-Oligo®分枝杆菌试验在痰液涂片阳性和阴性样本中鉴定分枝杆菌的评价
Pub Date : 2018-09-24 DOI: 10.31031/CJMI.2018.02.000533
G. Tarhan, İ. Ceyhan, H. Simsek, O. Kisa, Yusuf Ozay
Speed-oligo® Mycobacteria is an oligochromatographic test for the qualitative detection of Mycobacterium genus and the species of M. tuberculosis complex, directly in clinical samples. It is based on polymerase chain reaction targeting 16S rRNA and 16S-23S rRNA regions and double-reverse hybridization on a dipstick using probes bound to colloidal gold and to the membrane. In this study, we evaluated 25 type reference strains of mycobacteria (18 non-tuberculosis mycobacteria, M. tuberculosis H37Rv and M. tuberculosis H37Ra), 60 sputum samples (40 smear positive, 20 smear negative) collected from patient with suspected TB. All results were compared with microscopy, Löwenstein Jensen culture and Inno-Lipa (GenoType Mycobacterium CM/AS; Hain Lifescience, Germany). All smear positive sputum samples were positive with microscopy, culture and Speed-oligo® Mycobacteria. Of 20 smear negative sputum samples, 7 were culture positive. Of 7 culture positive samples, 3 were positive with microscopy and Speedoligo® Mycobacteria. It is not effective to identify for M. intermedium, M. kansasii and M. xenopi.
Speed-oligo®Mycobacteria是一种寡色谱检测方法,用于直接在临床样品中定性检测分枝杆菌属和结核分枝杆菌复合体种。它是基于针对16S rRNA和16S- 23s rRNA区域的聚合酶链反应,并使用结合在胶体金和膜上的探针在试纸上进行双反向杂交。本研究对疑似结核患者收集的25株分枝杆菌(18株非结核分枝杆菌、结核分枝杆菌H37Rv和结核分枝杆菌H37Ra)、60份痰标本(40份涂片阳性,20份涂片阴性)进行了评价。所有结果与显微镜、Löwenstein Jensen培养和Inno-Lipa(基因型分枝杆菌CM/AS;海恩生命科学,德国)。所有涂片阳性痰标本镜检、培养和Speed-oligo®分枝杆菌检测均为阳性。20份涂片阴性痰中,培养阳性7份。7份培养阳性样本中,3份镜检和Speedoligo®分枝杆菌阳性。中间芽孢杆菌、堪萨斯芽孢杆菌和色斑芽孢杆菌的鉴定效果不佳。
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引用次数: 0
Molecular Dıagnostic Tests Used in the Dıagnosis of Tuberculosis 用于结核病Dıagnosis的分子Dıagnostic测试
Pub Date : 2018-09-11 DOI: 10.31031/CJMI.2018.02.000532
G. Tarhan
The definitive diagnosis of tuberculosis (TB) is dependent on the isolation, identification and drug susceptibility testing of the causal agent Mycobacterium tuberculosis by cultivation. Smear microscopy has poor sensitivity and culture is slow to yield results. The resurgence of tuberculosis worldwide has been accompanied by an increase in the incidence of multidrug-resistant (MDR) tuberculosis on all continents. At the same time, a number of other nontuberculous mycobacterial (NTM) species are emerging as causes of disease. A quick and correct diagnosis of symptomatic tuberculosis is critical for the control of this serious disease. The nucleic acid amplification techniques (NAATs) and other molecular biology methods (i DNA hybridization, DNA sequencing, etc.) are essential in today’s laboratory practices for detection and characterization of mycobacteria. The use of NAATs in the routine detection of mycobacteria allowed a fast and accurate detection of the Mycobacterium species within 24 hours. The methods are widely used for the identification of mycobacteria, detection of the mutations in the resistance genes as well as the molecular epidemiological studies. The availability of new kits, and accumulated experience with nucleic acid amplification techniques for M. tuberculosis detection in most laboratories, have yielded improved sensitivity and specificity of these tests.
结核病的明确诊断依赖于病原菌结核分枝杆菌培养的分离、鉴定和药敏试验。涂片镜检灵敏度差,培养效果慢。在世界范围内,结核病的死灰复燃伴随着各大洲耐多药结核病发病率的上升。与此同时,许多其他非结核分枝杆菌(NTM)物种正在成为疾病的原因。对有症状结核病的快速和正确诊断对于控制这一严重疾病至关重要。核酸扩增技术(NAATs)和其他分子生物学方法(如DNA杂交、DNA测序等)在今天的实验室实践中对分枝杆菌的检测和表征是必不可少的。在分枝杆菌常规检测中使用NAATs可以在24小时内快速准确地检测分枝杆菌种类。该方法广泛应用于分枝杆菌的鉴定、耐药基因突变的检测以及分子流行病学研究。新试剂盒的可用性,以及大多数实验室在检测结核分枝杆菌的核酸扩增技术方面积累的经验,已经提高了这些检测的灵敏度和特异性。
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引用次数: 0
The Place Importance of Serologic Techniques in Tuberculosis Dıagnosis 血清学技术在肺结核中的地位与重要性Dıagnosis
Pub Date : 2018-08-20 DOI: 10.31031/CJMI.2018.02.000531
G. Tarhan
Diagnosing active TB accurately and rapidly is a key challenge for eradicating the TB epidemic.Conventional culture methods are slow and staining methods not sufficiently sensitive. Nucleic acid amplification techniques (NAATs) tend to be costly and in some cases lack sensitivity. Antibody detection tests (serological tests) have a long history and have been used successfully for the rapid diagnosis of many infectious diseases (e.g., HIV, syphilis, and viral hepatitis). TB serological tests almost exclusively rely on antibody recognition of antigens of Mycobacterium tuberculosis by the humoral immune response, as opposed to antigen recognition by the cellular immune response (e.g. interferon-gamma release assays. These tests use various modifications of enzyme-linked immunosorbent assay (ELISA) or immunochromatographic methods to detect different antibody classes. Cellular immunodiagnostics, including tuberculin skin test (TST) and Interferon-Gamma Release Assays (IGRAs) have been used to diagnose latent tuberculosis infection (LTBI). The TST is the only universally accepted test for the diagnosis of LTBI.
准确和迅速诊断活动性结核病是根除结核病流行的一项关键挑战。传统的培养方法是缓慢的,染色方法不够灵敏。核酸扩增技术(NAATs)往往是昂贵的,在某些情况下缺乏灵敏度。抗体检测试验(血清学试验)有着悠久的历史,并已成功地用于许多传染病(如艾滋病毒、梅毒和病毒性肝炎)的快速诊断。结核血清学试验几乎完全依赖于体液免疫反应对结核分枝杆菌抗原的抗体识别,而不是细胞免疫反应(如干扰素释放试验)对抗原的识别。这些测试使用酶联免疫吸附试验(ELISA)或免疫层析方法的各种修改来检测不同的抗体类别。细胞免疫诊断,包括结核菌素皮肤试验(TST)和干扰素γ释放试验(IGRAs)已被用于诊断潜伏性结核感染(LTBI)。TST是唯一被普遍接受的LTBI诊断测试。
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引用次数: 0
Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in Bastak 巴斯塔克皮肤利什曼病人库及感染的分子研究
Pub Date : 2018-07-31 DOI: 10.31031/CJMI.2018.02.000529
Mohammad Palangar, H. Jamali, Atefahekhosraubak
Leishmaniasis is one the six most important diseases in tropical areas. Study and research on various aspects of it is recommended and supported by the World Health Organization. Broom tail rodents (Rodentia: Muridae: Gerbillinae) are the most important reservoir hosts of parasite causes wet cutaneous leishmaniasis (or Zoonotic Cutaneous Leishmaniasis (ZCL) disease) means Leishmania major. Different species of rodents from different regions of Iran play role in maintenance of the parasite. The purpose of this research is molecular study of cutaneous leishmaniasis human reservoirs and infections in Bastak County, Hormozgan Province, Iran. Live traps were used to catch rodents. The rodents were anesthetized with chloroform in the field and four slides were prepared from earlobe of each animal by sanding method and their morphometric characteristics were completely measured and recorded. Rodent abdomen was opened with blade and the liver and the spleen of each sample were kept in a numbered container containing 70% ethanol. The prepared slides were examined with microscopic (Giemsa staining) and molecular methods. Finally, using Nested-PCR method, they were evaluated using species-specific primers (LIN R4, LIN 17, LIN 19). A total of 108 rodents from five species of three different genera were collected. Meriones persicus species with 26.9% was the predominant species. A contamination in T.indica species and two cases of contamination in two samples of female M.hurrianae were observed. L. major species was also identified. Broom tail rodents are abundance and have relatively high diversity in Bastak County. They live in proximity to human dwellings. Tatera indica and Meriones hurrianae species are introduced as the disease reservoir hosts in this area and use of molecular and PCR methods provides the possibility of faster diagnosis of parasite species.
利什曼病是热带地区六种最重要的疾病之一。世界卫生组织建议并支持对其各个方面进行研究。帚尾鼠(啮齿目:鼠科:沙billinae)是引起湿性皮肤利什曼病(或人畜共患皮肤利什曼病(ZCL)病)的寄生虫最重要的宿主。来自伊朗不同地区的不同种类的啮齿动物在寄生虫的维持中发挥作用。本研究的目的是对伊朗霍尔木兹甘省Bastak县皮肤利什曼病人类宿主和感染的分子研究。用活捕器捕捉啮齿动物。在野外用氯仿麻醉鼠,用砂磨法从每只鼠耳垂上制备4片载玻片,完整地测量并记录其形态特征。用刀片切开鼠腹,各标本的肝脏和脾脏保存在含有70%乙醇的编号容器中。对制备的载玻片进行显微(吉姆萨染色)和分子检测。最后,采用巢式pcr方法,利用物种特异性引物(LIN R4, LIN 17, LIN 19)对它们进行鉴定。共采集鼠类3属5种108只。优势种为西瓜,占26.9%。在2个样本中分别观察到1个籼稻种和2个雌性胡里奈尔蚊种的污染情况。L.主要种也有鉴定。巴塔克县帚尾鼠数量丰富,多样性较高。它们住在人类住所附近。引进了印度塔塔拉(Tatera indica)和飓风梅里安(Meriones hurrianae)作为该地区的疾病寄主,利用分子和PCR方法为寄生虫种类的快速诊断提供了可能。
{"title":"Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in Bastak","authors":"Mohammad Palangar, H. Jamali, Atefahekhosraubak","doi":"10.31031/CJMI.2018.02.000529","DOIUrl":"https://doi.org/10.31031/CJMI.2018.02.000529","url":null,"abstract":"Leishmaniasis is one the six most important diseases in tropical areas. Study and research on various aspects of it is recommended and supported by the World Health Organization. Broom tail rodents (Rodentia: Muridae: Gerbillinae) are the most important reservoir hosts of parasite causes wet cutaneous leishmaniasis (or Zoonotic Cutaneous Leishmaniasis (ZCL) disease) means Leishmania major. Different species of rodents from different regions of Iran play role in maintenance of the parasite. The purpose of this research is molecular study of cutaneous leishmaniasis human reservoirs and infections in Bastak County, Hormozgan Province, Iran. Live traps were used to catch rodents. The rodents were anesthetized with chloroform in the field and four slides were prepared from earlobe of each animal by sanding method and their morphometric characteristics were completely measured and recorded. Rodent abdomen was opened with blade and the liver and the spleen of each sample were kept in a numbered container containing 70% ethanol. The prepared slides were examined with microscopic (Giemsa staining) and molecular methods. Finally, using Nested-PCR method, they were evaluated using species-specific primers (LIN R4, LIN 17, LIN 19). A total of 108 rodents from five species of three different genera were collected. Meriones persicus species with 26.9% was the predominant species. A contamination in T.indica species and two cases of contamination in two samples of female M.hurrianae were observed. L. major species was also identified. Broom tail rodents are abundance and have relatively high diversity in Bastak County. They live in proximity to human dwellings. Tatera indica and Meriones hurrianae species are introduced as the disease reservoir hosts in this area and use of molecular and PCR methods provides the possibility of faster diagnosis of parasite species.","PeriodicalId":422893,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131281644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Magnetic Field Therapies in Association with the Relation between Macro and Microcosmos 磁场治疗与宏观与微观的关系
Pub Date : 2018-07-31 DOI: 10.31031/CJMI.2018.02.000528
I. Tunçer
From the concept of energy and matter to the magnetic fields in the universe, the relation between macro and micro points of view gives new perspectives for the understanding and management of life. While the discoveries of huge magnetic fields and mapping those are helping about galaxy and space formation [1,2], the magnetic fields of human body give information about the situation of cells, organs and the hole organism [3].
从能量和物质的概念到宇宙中的磁场,宏观和微观的观点之间的关系为认识和管理生命提供了新的视角。巨大磁场的发现和绘制有助于了解星系和空间的形成[1,2],而人体磁场则提供了有关细胞、器官和孔洞生物状况的信息[3]。
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引用次数: 0
Viable Monitoring During the Filling of a Terminally Sterilized Pharmaceutical Product-Risk Based Approach 终末灭菌药品灌装过程的可行性监测——基于风险的方法
Pub Date : 2018-07-18 DOI: 10.31031/CJMI.2018.02.000527
Ahmed Assem
This article addresses a risk based approach to establish a routine monitoring program of viable (I.e. microorganisms) in a controlled environment used to produce pharmaceutical products that are intended to be terminally sterilized. Current applicable quality system regulation & GMP require appropriate environment to be established, maintained and monitored for the manufacturing of the terminally sterilized pharmaceutical meanwhile the appropriate environmental monitoring plan is not clearly specified & a risk based approach will be a valuable tool to design a suitable program Table 1: Variable Very Low Low Normal High Very High Risk factor (A) Amount of microbial contamination on, or in, a source 1-2 (Class A) 3-4 (Class B) 5-6
本文介绍了一种基于风险的方法,在用于生产最终灭菌的药品的受控环境中建立活菌(即微生物)的常规监测程序。现行适用的质量体系法规和GMP要求为终端灭菌药品的生产建立、保持和监控适当的环境,同时没有明确规定适当的环境监控计划,基于风险的方法将是设计合适程序的有价值的工具。可变极低低正常高极高危险因子(A)源上或源内的微生物污染数量1-2 (A类)3-4 (B类)5-6
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引用次数: 0
Non-Thermal Decontamination of Food 食物的非热净化
Pub Date : 2018-06-15 DOI: 10.31031/CJMI.2018.01.000522
P. Birwal
In today’s generation the demand of fresh food with absence of harmful micro-organisms is increasing with world-wide awareness of the health benefits and risks associated with consumption of food. Meeting consumers’ expectation is necessity of the food industry. Food borne illness as a result of either post contamination or inefficient processing of food with pathogenic bacteria is a world-wide concern in this millennium. The European Food Safety Authority (EFSA) reported and documented Salmonella as the most common cause of food-borne outbreaks in the EU 3. Numbers of outbreaks of foodborne diseases due to salmonella spp., L. Monocytogens and E. coli have been reported and documented worldwide which caused loss of many lives in last few decades. United States Centers for disease control and prevention, reported that two-thirds of foodborne illness is caused by human enteric viruses i.e. nor virus (NoV), hepatitis A and E.
在今天这一代人中,对不含有害微生物的新鲜食品的需求正在增加,全世界都认识到与食品消费有关的健康益处和风险。满足消费者的期望是食品行业的需要。食源性疾病,无论是污染后或加工效率低下的致病菌的食品的结果是一个世界性的问题,在这个千年。欧洲食品安全局(EFSA)报告并记录了沙门氏菌是欧盟食源性疾病爆发的最常见原因。在过去的几十年里,由于沙门氏菌、单核增生乳杆菌和大肠杆菌引起的食源性疾病在世界范围内的暴发已被报道和记录,造成了许多人的生命损失。美国疾病控制和预防中心报告说,三分之二的食源性疾病是由人类肠道病毒引起的,即甲型肝炎和戊型肝炎病毒。
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引用次数: 0
Could Fast Response Curtail New Congo Ebola Virus Outbreak? 快速反应能否遏制刚果埃博拉病毒爆发?
Pub Date : 2018-06-11 DOI: 10.31031/CJMI.2018.01.000520
P. Akinwole
{"title":"Could Fast Response Curtail New Congo Ebola Virus Outbreak?","authors":"P. Akinwole","doi":"10.31031/CJMI.2018.01.000520","DOIUrl":"https://doi.org/10.31031/CJMI.2018.01.000520","url":null,"abstract":"","PeriodicalId":422893,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128137975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Cohesive Journal of Microbiology & Infectious Disease
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