Pub Date : 2016-01-01DOI: 10.15789/2220-7619-2016-4-359-365
Yu. V. Ostankova, A. V. Semenov, Z. K. Burkitbayev, T. N. Savchuk, A. A. Totolian
The prevalence of one of the hepatotropic virus, hepatitis B virus (HBV) remains a serious global health problem. Since hepatitis B is transmitted through contact with blood or other fluids of an infected person, blood safety is one of the major public health issues in regions with high virus prevalence. Observed in recent years the trend to a shift in the prevalence of various genotypes of HBV in different geographical areas due to immigration from regions of the world with a high incidence of hepatotropic viruses, makes doctors and epidemiologists to pay close attention to the epidemiological situation in neighboring countries. The aim of our work was to study the characteristics of the genetic structure of the HBV in primary donors in Astana, Kazakhstan. A total of 30 blood plasma samples from newly diagnosed hepatitis B (HBsAg+) of Astana. HBV DNA was detected in 27 samples out of 30. Based on the phylogenetic analysis of the isolates showed that among patients examined HBV identified mainly D genotype, which is the most common genotype of HBV in Central Asia. Thus HBV subtype predominant D1 (85,2%) compared to the HBV subtype D2 (3,7%) and subtype D3 (7,4%), in a single sample was detected HBV genotypes A subtype A1. High similarities identified isolates previously described in Iran, Sudan, Mongolia, Tunisia suggest numerous independent, perhaps mutual, the importation of the virus in the country, including in the major migration waves. First detected at the territory of Kazakhstan HBV subtype A1, uncharacteristic for the region, as well as subtypes D2 and D3, which have a high similarity with the nucleotide sequences of HBV in Russia, show cases of importation of the virus from other countries. Identification of the propagation and the role of «imported» genotypes of HBV virus in circulation may be essential for regions where the prevalence of hepatotropic viruses is high, and the genome structure and the way of their distribution sufficiently studied.
{"title":"ГЕНЕТИЧЕСКИЕ ВАРИАНТЫ ВИРУСА ГЕПАТИТА B У ПЕРВИЧНЫХ ДОНОРОВ В Г. АСТАНА, КАЗАХСТАН","authors":"Yu. V. Ostankova, A. V. Semenov, Z. K. Burkitbayev, T. N. Savchuk, A. A. Totolian","doi":"10.15789/2220-7619-2016-4-359-365","DOIUrl":"https://doi.org/10.15789/2220-7619-2016-4-359-365","url":null,"abstract":"The prevalence of one of the hepatotropic virus, hepatitis B virus (HBV) remains a serious global health problem. Since hepatitis B is transmitted through contact with blood or other fluids of an infected person, blood safety is one of the major public health issues in regions with high virus prevalence. Observed in recent years the trend to a shift in the prevalence of various genotypes of HBV in different geographical areas due to immigration from regions of the world with a high incidence of hepatotropic viruses, makes doctors and epidemiologists to pay close attention to the epidemiological situation in neighboring countries. The aim of our work was to study the characteristics of the genetic structure of the HBV in primary donors in Astana, Kazakhstan. A total of 30 blood plasma samples from newly diagnosed hepatitis B (HBsAg+) of Astana. HBV DNA was detected in 27 samples out of 30. Based on the phylogenetic analysis of the isolates showed that among patients examined HBV identified mainly D genotype, which is the most common genotype of HBV in Central Asia. Thus HBV subtype predominant D1 (85,2%) compared to the HBV subtype D2 (3,7%) and subtype D3 (7,4%), in a single sample was detected HBV genotypes A subtype A1. High similarities identified isolates previously described in Iran, Sudan, Mongolia, Tunisia suggest numerous independent, perhaps mutual, the importation of the virus in the country, including in the major migration waves. First detected at the territory of Kazakhstan HBV subtype A1, uncharacteristic for the region, as well as subtypes D2 and D3, which have a high similarity with the nucleotide sequences of HBV in Russia, show cases of importation of the virus from other countries. Identification of the propagation and the role of «imported» genotypes of HBV virus in circulation may be essential for regions where the prevalence of hepatotropic viruses is high, and the genome structure and the way of their distribution sufficiently studied.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"6 1","pages":"359-365"},"PeriodicalIF":0.4,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.15789/2220-7619-2016-4-389-394
Ya. V. Podkopaev, L. V. Domotenko, A. N. Kruglov, I. V. Ryabchenko, K. V. Detushev, T. P. Morozova, A. P. Shepelin
The State Research Center for Applied Microbiology and Biotechnology has designed two nutrient media — chocolate agar and PBM-agar to isolate pathogens of purulent bacterial meningitis (PBM). In our previous research using collected microbial strains the media were shown to be highly susceptible and to provide the growth of Neisseria meningiti-dis, Streptococcus pneumoniae and Haemophilus influenzae strains, when inoculated with microbial suspensions containing single cells. When isolating Haemophilus influenzae , meningococci, and pneumococci the use of selective additives in both media assures selective isolation of required microorganisms, inhibiting contaminants. The objective of this research was to assess the media in bacteriological tests of clinical samples collected from the upper and lower respiratory tract in humans. The bacteriological plating of throat smear specimens (n = 90) from children and adults at the age of 0 to 66 with disorder of the upper respiratory tract on chocolate agar, PBM-agar and on a control medium in the absence of selective additives resulted in the equal amount of microbial cultures isolated. Of 154 isolated cultures 2, 23 and 9 were attributed to Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae , respectively. The plating of throat smears (n = 10) from healthy people at the age of 30 to 55 on the analyzable and control media in the presence of additives allowed us to selectively isolate Haemophilus influenzae and Streptococcus pneumoniae cultures without a quantitative loss, with contaminants inhibited. By their growth characteristics chocolate agar and PBM-agar were highly competitive with reference media being used in clinical practice for isolating main causative agents of purulent bacterial meningitis.
{"title":"СРАВНИТЕЛЬНАЯ ОЦЕНКА ПИТАТЕЛЬНЫХ СРЕД ДЛЯ ВЫДЕЛЕНИЯ ВОЗБУДИТЕЛЕЙ ГНОЙНЫХ БАКТЕРИАЛЬНЫХ МЕНИНГИТОВ","authors":"Ya. V. Podkopaev, L. V. Domotenko, A. N. Kruglov, I. V. Ryabchenko, K. V. Detushev, T. P. Morozova, A. P. Shepelin","doi":"10.15789/2220-7619-2016-4-389-394","DOIUrl":"https://doi.org/10.15789/2220-7619-2016-4-389-394","url":null,"abstract":"The State Research Center for Applied Microbiology and Biotechnology has designed two nutrient media — chocolate agar and PBM-agar to isolate pathogens of purulent bacterial meningitis (PBM). In our previous research using collected microbial strains the media were shown to be highly susceptible and to provide the growth of Neisseria meningiti-dis, Streptococcus pneumoniae and Haemophilus influenzae strains, when inoculated with microbial suspensions containing single cells. When isolating Haemophilus influenzae , meningococci, and pneumococci the use of selective additives in both media assures selective isolation of required microorganisms, inhibiting contaminants. The objective of this research was to assess the media in bacteriological tests of clinical samples collected from the upper and lower respiratory tract in humans. The bacteriological plating of throat smear specimens (n = 90) from children and adults at the age of 0 to 66 with disorder of the upper respiratory tract on chocolate agar, PBM-agar and on a control medium in the absence of selective additives resulted in the equal amount of microbial cultures isolated. Of 154 isolated cultures 2, 23 and 9 were attributed to Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae , respectively. The plating of throat smears (n = 10) from healthy people at the age of 30 to 55 on the analyzable and control media in the presence of additives allowed us to selectively isolate Haemophilus influenzae and Streptococcus pneumoniae cultures without a quantitative loss, with contaminants inhibited. By their growth characteristics chocolate agar and PBM-agar were highly competitive with reference media being used in clinical practice for isolating main causative agents of purulent bacterial meningitis.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"6 1","pages":"389-394"},"PeriodicalIF":0.4,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-30DOI: 10.15789/2220-7619-2015-3-279-284
N. S. Bobyreva, G. N. Degteva
The Nenets Autonomous District (NAD) refers to the territories of the Russia’s Far North. The country is the only region of Russia, where there are still no roads. The extreme weather conditions, as well as residential patterns in the NAD reduce the availability of specialized medical care, as indigenous people and other nationalities living in the district, which requires the use of health care management specific forms. The article presents data on giardiasis studies amongst different categories of the NAD population, obtained as a result of the population surveys in remote regions of the Russian Federation Arctic zone. Samples studied were serum and faecal emulsion. The study used the following laboratory methods: for serum — Giardia lamblia antibody screening method using ELISA kit, emulsion stool — native smear microscopy analysis, the method of enrichment using disposable concentrators «Parasep» immunological diagnostic methods based on the detection of specific lamblia antigen in faeces using ELISA kits. For statistics was used descriptive statistics analysis (mean values, the percentage error of the mean, the construction of the trend line) with program SPSS 20.00, Excel 2010, the method of analysis of official statistical reporting hospitals survey on giardiasis, data of Rospotrebnadzor (Russian Sanitary service) for NAD and data from own research from 2002 to 2013. Just in the process of research carried out research on the 10 356 giardiasis, registered diseases by 3470 cases of giardiasis, accounting for 99.14% of the total number of protozoonosises registered and 37.4% of all registered in the NAD parasitosis. The comparison was made among the population of the county towns, home to both the indigenous people, nomadic, and representatives of other ethnic groups, sedentary with urban population of the city of Naryan-Mar. The article analyzes the incidence rates for giardiasis according to district official statistical reporting compared with the incidence in the Russian Federation for the same period of time. Statistical analysis was performed using the software package Excel 2010. The study found an association of giardiasis prevalence in the population of the NAD on criteria such as location, gender, occupation and age structure examined.
{"title":"Анализ данных лабораторного обследования на лямблиоз у различных групп населения Ненецкого автономного округа","authors":"N. S. Bobyreva, G. N. Degteva","doi":"10.15789/2220-7619-2015-3-279-284","DOIUrl":"https://doi.org/10.15789/2220-7619-2015-3-279-284","url":null,"abstract":"The Nenets Autonomous District (NAD) refers to the territories of the Russia’s Far North. The country is the only region of Russia, where there are still no roads. The extreme weather conditions, as well as residential patterns in the NAD reduce the availability of specialized medical care, as indigenous people and other nationalities living in the district, which requires the use of health care management specific forms. The article presents data on giardiasis studies amongst different categories of the NAD population, obtained as a result of the population surveys in remote regions of the Russian Federation Arctic zone. Samples studied were serum and faecal emulsion. The study used the following laboratory methods: for serum — Giardia lamblia antibody screening method using ELISA kit, emulsion stool — native smear microscopy analysis, the method of enrichment using disposable concentrators «Parasep» immunological diagnostic methods based on the detection of specific lamblia antigen in faeces using ELISA kits. For statistics was used descriptive statistics analysis (mean values, the percentage error of the mean, the construction of the trend line) with program SPSS 20.00, Excel 2010, the method of analysis of official statistical reporting hospitals survey on giardiasis, data of Rospotrebnadzor (Russian Sanitary service) for NAD and data from own research from 2002 to 2013. Just in the process of research carried out research on the 10 356 giardiasis, registered diseases by 3470 cases of giardiasis, accounting for 99.14% of the total number of protozoonosises registered and 37.4% of all registered in the NAD parasitosis. The comparison was made among the population of the county towns, home to both the indigenous people, nomadic, and representatives of other ethnic groups, sedentary with urban population of the city of Naryan-Mar. The article analyzes the incidence rates for giardiasis according to district official statistical reporting compared with the incidence in the Russian Federation for the same period of time. Statistical analysis was performed using the software package Excel 2010. The study found an association of giardiasis prevalence in the population of the NAD on criteria such as location, gender, occupation and age structure examined.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"5 1","pages":"279-284"},"PeriodicalIF":0.4,"publicationDate":"2015-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-06-28DOI: 10.15789/2220-7619-2015-2-148-156
G. A. Borisov, A. Savchenko, I. V. Kudryavtsev
The aim of the investigation was to select using cluster analysis and comparatively characterize immune disorders types in acute and chronic viral infections. Patients with acute and chronic viral infections (n = 896) were examined: 77 patients with acute viral hepatitis B, 94 — chronic viral hepatitis B, 119 — chronic hepatitis C, 531 — recurrent herpes, 75 — human papillomavirus infection. Healthy persons (n = 466) were examined as control. The research of blood lymphocyte phenotype was performed by flow cytometry. Four-color immunophenotyping were used in the following panels: Т-lymphocytes (CD3+CD19–CD16/56–CD45+), Т-helpers (CD3+CD4+CD45+), cytotoxic Т-cells (CD3+CD8+CD45+), NKcells (CD3–CD16/56+CD45+), B-lymphocytes (CD3–CD19+CD16/56+CD45+). Absolute values were obtained on a dualplatform technology using the results of haematological analysis. The immunoglobulin concentrations were determined by ELISA. The clustering was performed by a single linkage method. The number of clusters was determined on the basis of calculating the values of the Euclidean distance between the mean group values. It was found that the parameters, characterizing the functional state of the various parts of the immune system in acute and chronic viral infections, considerable diversity values. Custer analysis allows to allocate 6 immunotypes defined different states of innate and adaptive immunity: characterized by activation of the innate (increasing the number of neutrophils and NK-cells) and adaptive immunity humoral response (increasing the concentration of IgG), characterized by hyperreaction of adaptive immunity (a significant increase in the concentration of IgG), discoordinated (multidirectional changes in the values of immunological parameters), immunodeficiency and unresponsiveness (did not differ from the control parameters) immunotypes. It is proved that in patients with viral infections most often determined by the “unresponsiveness” immunotype (40,5%), as well as humoral immunodeficiency (24,9%) and adaptive immune reaction (24,5%). A group of patients with chronic viral hepatitis B and C is allocated separately in which more than 10% of the detected adaptive immunity overreaction that is probably due to the development of chronic hepatitis. These immunotypes can be regarded as different pathogenetic variants of the course of acute and chronic viral infections. Healthy people often had unresponsiveness or immunodeficiency immunotypes, that is their immune system is out of activation. Stratification of patients with viral infections by immunotypes will increase the effectiveness of treatment and implement personalized approaches to diagnosis and treatment of functional disorders of the immune system.
{"title":"ОСОБЕННОСТИ ИММУННОГО РЕАГИРОВАНИЯ ПРИ ВИРУСНЫХ ИНФЕКЦИЯХ","authors":"G. A. Borisov, A. Savchenko, I. V. Kudryavtsev","doi":"10.15789/2220-7619-2015-2-148-156","DOIUrl":"https://doi.org/10.15789/2220-7619-2015-2-148-156","url":null,"abstract":"The aim of the investigation was to select using cluster analysis and comparatively characterize immune disorders types in acute and chronic viral infections. Patients with acute and chronic viral infections (n = 896) were examined: 77 patients with acute viral hepatitis B, 94 — chronic viral hepatitis B, 119 — chronic hepatitis C, 531 — recurrent herpes, 75 — human papillomavirus infection. Healthy persons (n = 466) were examined as control. The research of blood lymphocyte phenotype was performed by flow cytometry. Four-color immunophenotyping were used in the following panels: Т-lymphocytes (CD3+CD19–CD16/56–CD45+), Т-helpers (CD3+CD4+CD45+), cytotoxic Т-cells (CD3+CD8+CD45+), NKcells (CD3–CD16/56+CD45+), B-lymphocytes (CD3–CD19+CD16/56+CD45+). Absolute values were obtained on a dualplatform technology using the results of haematological analysis. The immunoglobulin concentrations were determined by ELISA. The clustering was performed by a single linkage method. The number of clusters was determined on the basis of calculating the values of the Euclidean distance between the mean group values. It was found that the parameters, characterizing the functional state of the various parts of the immune system in acute and chronic viral infections, considerable diversity values. Custer analysis allows to allocate 6 immunotypes defined different states of innate and adaptive immunity: characterized by activation of the innate (increasing the number of neutrophils and NK-cells) and adaptive immunity humoral response (increasing the concentration of IgG), characterized by hyperreaction of adaptive immunity (a significant increase in the concentration of IgG), discoordinated (multidirectional changes in the values of immunological parameters), immunodeficiency and unresponsiveness (did not differ from the control parameters) immunotypes. It is proved that in patients with viral infections most often determined by the “unresponsiveness” immunotype (40,5%), as well as humoral immunodeficiency (24,9%) and adaptive immune reaction (24,5%). A group of patients with chronic viral hepatitis B and C is allocated separately in which more than 10% of the detected adaptive immunity overreaction that is probably due to the development of chronic hepatitis. These immunotypes can be regarded as different pathogenetic variants of the course of acute and chronic viral infections. Healthy people often had unresponsiveness or immunodeficiency immunotypes, that is their immune system is out of activation. Stratification of patients with viral infections by immunotypes will increase the effectiveness of treatment and implement personalized approaches to diagnosis and treatment of functional disorders of the immune system.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"5 1","pages":"148-156"},"PeriodicalIF":0.4,"publicationDate":"2015-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-06-27DOI: 10.15789/2220-7619-2015-2-113-130
E. P. Kisseleva
Review covers modern data on relationships of normal intestinal microbiota and immune system. Possibility to maintain the residence of large numbers of symbiotic bateria at mucosal surfaces of the body is regarded as a separate and independent immunological function named acceptive immunity. Basic effector arms of protective (defense against pathogens) and acceptive immunity (symbiotic relationships) are compared. Acceptive immunity differs from protective one in the absence of inflammation where all complex of immune reactions occurs in the context of physiological process. Several homeostatic mechanisms that provide crosstalk with symbiotic bacteria at the epithelial surfaces, innate and adaptive immunity are described. The main immunological strategies towards symbiotic bacteria are support of microbial community from one hand, and providing of host defense, from the other hand. The key step of this interaction is sensing of soluble microbial products via pattern-recognition receptors on the host cells. Basic innate immune response consists of mucus production and synthesis of antimicrobial peptides by barrier epithelial cells as well as maintenance of specific anti-inflammatory microenvironment. The main adaptive response is synthesis of secretory immunoglobulin A that is produced to the intestinal lumen and interacts with bacteria. At the same time, immunoglobulin A does not make any damage for commensals. Moreover this factor plays important role in symbiotic relationships. The following promicrobial functions of immunoglobulin A are suggested: participation in biofilm formation, discrimination of intestinal bacteria for fixed and free-living populations as well as facilitation of microbial transport through M cells. Mucosal homeostasis is supported by the development of immunological tolerance with participation of T regulatory cells. Main mechanisms of the development and maintenance of specific tolerance towards antigens of normal microbiota are discussed. Modern data on the participation of two main populations of T-regulatory cells are cited — thymic cells and cells induced in periphery. It is now accepted, that development of specific tolerance to microbial and food antigens plays important role in prevention of autoimmune and allergic diseases.
{"title":"АКЦЕПТИВНЫЙ ИММУНИТЕТ — ОСНОВА СИМБИОТИЧЕСКИХ ВЗАИМООТНОШЕНИЙ","authors":"E. P. Kisseleva","doi":"10.15789/2220-7619-2015-2-113-130","DOIUrl":"https://doi.org/10.15789/2220-7619-2015-2-113-130","url":null,"abstract":"Review covers modern data on relationships of normal intestinal microbiota and immune system. Possibility to maintain the residence of large numbers of symbiotic bateria at mucosal surfaces of the body is regarded as a separate and independent immunological function named acceptive immunity. Basic effector arms of protective (defense against pathogens) and acceptive immunity (symbiotic relationships) are compared. Acceptive immunity differs from protective one in the absence of inflammation where all complex of immune reactions occurs in the context of physiological process. Several homeostatic mechanisms that provide crosstalk with symbiotic bacteria at the epithelial surfaces, innate and adaptive immunity are described. The main immunological strategies towards symbiotic bacteria are support of microbial community from one hand, and providing of host defense, from the other hand. The key step of this interaction is sensing of soluble microbial products via pattern-recognition receptors on the host cells. Basic innate immune response consists of mucus production and synthesis of antimicrobial peptides by barrier epithelial cells as well as maintenance of specific anti-inflammatory microenvironment. The main adaptive response is synthesis of secretory immunoglobulin A that is produced to the intestinal lumen and interacts with bacteria. At the same time, immunoglobulin A does not make any damage for commensals. Moreover this factor plays important role in symbiotic relationships. The following promicrobial functions of immunoglobulin A are suggested: participation in biofilm formation, discrimination of intestinal bacteria for fixed and free-living populations as well as facilitation of microbial transport through M cells. Mucosal homeostasis is supported by the development of immunological tolerance with participation of T regulatory cells. Main mechanisms of the development and maintenance of specific tolerance towards antigens of normal microbiota are discussed. Modern data on the participation of two main populations of T-regulatory cells are cited — thymic cells and cells induced in periphery. It is now accepted, that development of specific tolerance to microbial and food antigens plays important role in prevention of autoimmune and allergic diseases.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"5 1","pages":"113-130"},"PeriodicalIF":0.4,"publicationDate":"2015-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-02-03DOI: 10.15789/2220-7619-2014-4-359-364
T. M. Lyuboshenko, T. I. Dolgikh
The peculiarities of clinical signs, immune and interferon status in 180 patients with laboratory confirmed infection of varying severity, caused by herpes simplex virus (VSHI) have been studied. It was determined that frequency of bacterial infections is increased in patients with more severe clinical forms of VSHI. In patients with mild course furunculosis was more often detected than in other groups. In patients with moderate course of VSHI vaginal candidiasis was more common. In patients with severe VSHI course the combination of labial and genital herpes as well as infection caused by the human papilloma virus were more prevalent. In case of severe infection occurred an increased frequency of dysbiosis, fatigue, low grade temperature, iron deficiency anemia and malignancies. The highest frequency of allergic reactions is observed in patients with moderate course of VSHI. The autoimmune syndrome manifestations were not depend on the severity of VSHI. The degree of reduction of cell immunity and disorders in the system of interferon were closely related to severity of VSHI course.
{"title":"КЛИНИКО-ИММУНОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА БОЛЬНЫХ С ГЕРПЕСВИРУСНОЙ ИНФЕКЦИЕЙ РАЗЛИЧНОЙ ТЯЖЕСТИ","authors":"T. M. Lyuboshenko, T. I. Dolgikh","doi":"10.15789/2220-7619-2014-4-359-364","DOIUrl":"https://doi.org/10.15789/2220-7619-2014-4-359-364","url":null,"abstract":"The peculiarities of clinical signs, immune and interferon status in 180 patients with laboratory confirmed infection of varying severity, caused by herpes simplex virus (VSHI) have been studied. It was determined that frequency of bacterial infections is increased in patients with more severe clinical forms of VSHI. In patients with mild course furunculosis was more often detected than in other groups. In patients with moderate course of VSHI vaginal candidiasis was more common. In patients with severe VSHI course the combination of labial and genital herpes as well as infection caused by the human papilloma virus were more prevalent. In case of severe infection occurred an increased frequency of dysbiosis, fatigue, low grade temperature, iron deficiency anemia and malignancies. The highest frequency of allergic reactions is observed in patients with moderate course of VSHI. The autoimmune syndrome manifestations were not depend on the severity of VSHI. The degree of reduction of cell immunity and disorders in the system of interferon were closely related to severity of VSHI course.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"4 1","pages":"359-364"},"PeriodicalIF":0.4,"publicationDate":"2015-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-02-03DOI: 10.15789/2220-7619-2014-4-339-346
A. A. Zutkis, B. Milman, A. Dmitriev
In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strains compared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.
{"title":"РОЛЬ ГЕНА mutR В МЕТАБОЛИЗМЕ И ВИРУЛЕНТНОСТИ ШТАММОВ STREPTOCOCCUS PYOGENES ГЕНОТИПА emm12","authors":"A. A. Zutkis, B. Milman, A. Dmitriev","doi":"10.15789/2220-7619-2014-4-339-346","DOIUrl":"https://doi.org/10.15789/2220-7619-2014-4-339-346","url":null,"abstract":"In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strains compared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"4 1","pages":"339-346"},"PeriodicalIF":0.4,"publicationDate":"2015-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-02-01DOI: 10.15789/2220-7619-2014-4-319-330
B. Vishnevskiy, O. Manicheva, P. Yablonskiy
{"title":"MYCOBACTERIUM TUBERCULOSIS VIRULENCE","authors":"B. Vishnevskiy, O. Manicheva, P. Yablonskiy","doi":"10.15789/2220-7619-2014-4-319-330","DOIUrl":"https://doi.org/10.15789/2220-7619-2014-4-319-330","url":null,"abstract":"","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"4 1","pages":"319-330"},"PeriodicalIF":0.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.15789/2220-7619-2015-1-63-70
A. Savchenko, A. Borisov, D. E. Zdzitoveckij, I. Kudryavtsev
{"title":"THE CELLULAR AND HUMORAL IMMUNITY STATE DEPENDING ON THE OUTCOME OF A WIDESPREAD PURULENT PERITONITIS","authors":"A. Savchenko, A. Borisov, D. E. Zdzitoveckij, I. Kudryavtsev","doi":"10.15789/2220-7619-2015-1-63-70","DOIUrl":"https://doi.org/10.15789/2220-7619-2015-1-63-70","url":null,"abstract":"","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"5 1","pages":"63-70"},"PeriodicalIF":0.4,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.15789/2220-7619-2015-1-15-26
B. G. Andrukov, L. Somova, N. Timchenko
{"title":"STRATEGY OF PROGRAMMED CELL DEATH IN PROKARYOTES","authors":"B. G. Andrukov, L. Somova, N. Timchenko","doi":"10.15789/2220-7619-2015-1-15-26","DOIUrl":"https://doi.org/10.15789/2220-7619-2015-1-15-26","url":null,"abstract":"","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"44 1","pages":"15-26"},"PeriodicalIF":0.4,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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