Pub Date : 2020-11-25DOI: 10.1177/2320206820958239
Rhythm Bains, A. Tikku, A. Chandra, W. Ali, Promila Verma
Aim: To assess the before- and after-treatment levels of glutathione (GSH) in the serum of patients with chronic apical periodontitis. Materials and Methods: The study involved 20 systemically healthy patients with radiographic evidence of chronic apical periodontitis in at least one tooth, of which 11 were finally assessed. 10 mL of the patient’s venous blood was obtained before commencing the endodontic treatment to assess the pre-operative serum GSH levels (T0). The canals cleaned and shaped, and irrigated with 2.5% sodium hypochlorite, and filled with a calcium hydroxide based intracanal medicament. At the subsequent appointment after one week, teeth were obturated. The patients were recalled 30 days after the completion of endodontic therapy, and blood samples were assessed again at time frame T1 for serum GSH. The difference in pre- and post-treatment values for T0 and T1 was statistically analyzed. Results: The mean age of the subjects (N = 11) was 21.09±3.24 years, with 19.50±0.71 years in female group and 21.44±3.50 years in the male group. The mean GSH at T0 was 11.73±8.30 units, which was increased to 14.04±13.96 units at T1. However, no significant change was observed in the GSH level from T0 to T1 (change = –2.30, t-value = –0.49, P = .635). Conclusion: The results of this study suggest that endodontic therapy may play a role in restoring the systemic oxidative balance, as seen by the increase in post-treatment serum GSH levels.
{"title":"Effect of Endodontic Treatment on Serum Glutathione (GSH) Levels in Patients with Chronic Apical Periodontitis: A Clinical Pilot Study","authors":"Rhythm Bains, A. Tikku, A. Chandra, W. Ali, Promila Verma","doi":"10.1177/2320206820958239","DOIUrl":"https://doi.org/10.1177/2320206820958239","url":null,"abstract":"Aim: To assess the before- and after-treatment levels of glutathione (GSH) in the serum of patients with chronic apical periodontitis. Materials and Methods: The study involved 20 systemically healthy patients with radiographic evidence of chronic apical periodontitis in at least one tooth, of which 11 were finally assessed. 10 mL of the patient’s venous blood was obtained before commencing the endodontic treatment to assess the pre-operative serum GSH levels (T0). The canals cleaned and shaped, and irrigated with 2.5% sodium hypochlorite, and filled with a calcium hydroxide based intracanal medicament. At the subsequent appointment after one week, teeth were obturated. The patients were recalled 30 days after the completion of endodontic therapy, and blood samples were assessed again at time frame T1 for serum GSH. The difference in pre- and post-treatment values for T0 and T1 was statistically analyzed. Results: The mean age of the subjects (N = 11) was 21.09±3.24 years, with 19.50±0.71 years in female group and 21.44±3.50 years in the male group. The mean GSH at T0 was 11.73±8.30 units, which was increased to 14.04±13.96 units at T1. However, no significant change was observed in the GSH level from T0 to T1 (change = –2.30, t-value = –0.49, P = .635). Conclusion: The results of this study suggest that endodontic therapy may play a role in restoring the systemic oxidative balance, as seen by the increase in post-treatment serum GSH levels.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"31 1","pages":"81 - 85"},"PeriodicalIF":0.7,"publicationDate":"2020-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84513439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-25DOI: 10.1177/2320206820968448
B. Sekar, K. Indrapriyadharshini, M. Ambika, R. Saranyan, M. Nirmal, Sathick Manzoor
Aim: Survivin is a multifunctional protein chiefly involved in apoptosis and cell cycle regulation. Increased expression of survivin in tumors and fetal tissue determines its antiapoptotic activity. The aim of the study is to identify the immunoexpression of survivin in metastatic and nonmetastatic oral squamous cell carcinoma (OSCC) and also to evaluate and compare the expression of survivin in metastatic and nonmetastatic OSCC of buccal mucosa. Materials and Methods: In total, 40 histopathologically proven cases of OSCC, including 20 metastatic and 20 nonmetastatic cases, are selected. Among the 20 metastatic and nonmetastatic cases, 10 well-differentiated and 10 moderately differentiated squamous cell carcinoma cases were included and were subjected to immunohistochemical staining for survivin expression. The results were analyzed by SPSS version 11.5 using chi-square test. Results: The expression of survivin in metastatic and nonmetastatic tumors is 15%–70% and 15%–60%, respectively. When comparing the cases of moderately differentiated squamous cell carcinoma in metastatic and nonmetastatic tumors, 70% cases show moderate staining intensity. Conclusion: The survivin expression was comparatively high in metastatic OSCC. Also based on the aforementioned results, survivin expression was high in increasing grades of OSCC.
{"title":"Survivin Expression in Metastatic and Nonmetastatic Oral Squamous Cell Carcinoma: A Comparative Study","authors":"B. Sekar, K. Indrapriyadharshini, M. Ambika, R. Saranyan, M. Nirmal, Sathick Manzoor","doi":"10.1177/2320206820968448","DOIUrl":"https://doi.org/10.1177/2320206820968448","url":null,"abstract":"Aim: Survivin is a multifunctional protein chiefly involved in apoptosis and cell cycle regulation. Increased expression of survivin in tumors and fetal tissue determines its antiapoptotic activity. The aim of the study is to identify the immunoexpression of survivin in metastatic and nonmetastatic oral squamous cell carcinoma (OSCC) and also to evaluate and compare the expression of survivin in metastatic and nonmetastatic OSCC of buccal mucosa. Materials and Methods: In total, 40 histopathologically proven cases of OSCC, including 20 metastatic and 20 nonmetastatic cases, are selected. Among the 20 metastatic and nonmetastatic cases, 10 well-differentiated and 10 moderately differentiated squamous cell carcinoma cases were included and were subjected to immunohistochemical staining for survivin expression. The results were analyzed by SPSS version 11.5 using chi-square test. Results: The expression of survivin in metastatic and nonmetastatic tumors is 15%–70% and 15%–60%, respectively. When comparing the cases of moderately differentiated squamous cell carcinoma in metastatic and nonmetastatic tumors, 70% cases show moderate staining intensity. Conclusion: The survivin expression was comparatively high in metastatic OSCC. Also based on the aforementioned results, survivin expression was high in increasing grades of OSCC.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"45 1","pages":"75 - 80"},"PeriodicalIF":0.7,"publicationDate":"2020-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74493543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-17DOI: 10.1177/2320206820972239
F. Rahman, S. Jahan, M. Shaikh, E. Apu
1 Faculty of Dentistry, Prosthodontics Units, Universiti Sains Malaysia (Health Campus), Kota Bharu, Kelantan, Malaysia 2 Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, Glasgow, Scotland, UK 3 Department of Otolaryngology—Head and Neck Surgery, Schulich School of Medicine & Dentistry, Western University, London, Ontario, Canada 4 Department of Biomedical Engineering, Institute of Quantitative Health Science (IQ), Michigan State University, East Lansing, MI, USA. 5 Intervention Center, Oslo University Hospital, Rikshospitalet, Nydalen, Oslo, Norway
{"title":"Use of Antivirals in Denture: A Potential Approach in Dental Practice","authors":"F. Rahman, S. Jahan, M. Shaikh, E. Apu","doi":"10.1177/2320206820972239","DOIUrl":"https://doi.org/10.1177/2320206820972239","url":null,"abstract":"1 Faculty of Dentistry, Prosthodontics Units, Universiti Sains Malaysia (Health Campus), Kota Bharu, Kelantan, Malaysia 2 Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, Glasgow, Scotland, UK 3 Department of Otolaryngology—Head and Neck Surgery, Schulich School of Medicine & Dentistry, Western University, London, Ontario, Canada 4 Department of Biomedical Engineering, Institute of Quantitative Health Science (IQ), Michigan State University, East Lansing, MI, USA. 5 Intervention Center, Oslo University Hospital, Rikshospitalet, Nydalen, Oslo, Norway","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"136 1","pages":"5 - 6"},"PeriodicalIF":0.7,"publicationDate":"2020-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77458399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-06DOI: 10.1177/2320206820960860
MH Buyukcavus, G. Kocakara
Aim: To examine pharyngeal airway dimensions and hyoid bone position according to the subgroups of Class III malocclusion. Materials and Methods: This retrospective study consisted of patients divided into three subgroups with skeletal Class III malocclusion. The study included a total of 151 individuals (61 females and 90 males). The authors divided individuals with skeletal Class III malocclusion into three subgroups: maxillary retrognathia, mandibular prognathia, and combined. The study’s cephalometric analysis used eight nasopharyngeal, seven oropharyngeal, two hypopharyngeal, nine hyoid, and four area measurements. One-way analysis of variance was used to evaluate patients. Tukey’s post-hoc tests were used for bilateral comparisons of significant parameters. The results were considered statistically significant at a P < .05 significance level. Results: The study found no significant differences between the groups’ pharyngeal airway and area measurements (P > .05). When the authors evaluated hyoid bone position, a statistically significant difference was found between the three groups’ Hy-A (mm), Hy-S (mm), Hy-SN (mm), and Hy-FH (mm) measurements (P < .05). Conclusion: Linear and areal pharyngeal airway dimensions are similar in subgroups of Class III malocclusions, while the hyoid bone is vertically higher in individuals with maxillary retrognathia.
{"title":"Comparing Pharyngeal Airway Dimensions and Hyoid Bone Position in the Subgroups of Skeletal Class III Malocclusions: A Cephalometric Study","authors":"MH Buyukcavus, G. Kocakara","doi":"10.1177/2320206820960860","DOIUrl":"https://doi.org/10.1177/2320206820960860","url":null,"abstract":"Aim: To examine pharyngeal airway dimensions and hyoid bone position according to the subgroups of Class III malocclusion. Materials and Methods: This retrospective study consisted of patients divided into three subgroups with skeletal Class III malocclusion. The study included a total of 151 individuals (61 females and 90 males). The authors divided individuals with skeletal Class III malocclusion into three subgroups: maxillary retrognathia, mandibular prognathia, and combined. The study’s cephalometric analysis used eight nasopharyngeal, seven oropharyngeal, two hypopharyngeal, nine hyoid, and four area measurements. One-way analysis of variance was used to evaluate patients. Tukey’s post-hoc tests were used for bilateral comparisons of significant parameters. The results were considered statistically significant at a P < .05 significance level. Results: The study found no significant differences between the groups’ pharyngeal airway and area measurements (P > .05). When the authors evaluated hyoid bone position, a statistically significant difference was found between the three groups’ Hy-A (mm), Hy-S (mm), Hy-SN (mm), and Hy-FH (mm) measurements (P < .05). Conclusion: Linear and areal pharyngeal airway dimensions are similar in subgroups of Class III malocclusions, while the hyoid bone is vertically higher in individuals with maxillary retrognathia.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"9 1","pages":"86 - 94"},"PeriodicalIF":0.7,"publicationDate":"2020-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87651997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-06DOI: 10.1177/2320206820960862
A. Murugesan, B. Sekar, R. Saranyan, E. Manivannan, M. Rajmohan
Aim: Cancer stem cells (CSCs) or tumor-initiating cells have self-renewal and uncontrolled tumor growth capacity that promotes metastasis and recurrence. Challenges in anticancer have found a lateral dimension of treatment against a new pattern of tumor microcirculation, known as vasculogenic mimicry (VM), involved in cancer progression. Increasing evidence suggest that CSCs are involved in the formation of VM. In this review the correlation between CSCs and VM formation is been enlightened. Materials and Methods: The literature search was done in Medline, PubMed, Wiley, Science Direct, and Scopus. The keywords used for database search were cancer stem cells, vasculogenic mimicry, and anticancer therapy. Results: A total of 112 articles appeared from various sources, of which 102 were subjected for screening and 20 were related to the research objective. Conclusion: Based on the literature a positive correlation exists between CSC and VM, which plays a key role in tumor progression, and hence, can serve as a potential target in anticancer therapy.
{"title":"A Review on Cancer Stem Cells in Vasculogenic Mimicry Formation: A New Dimension for Targeted Therapy","authors":"A. Murugesan, B. Sekar, R. Saranyan, E. Manivannan, M. Rajmohan","doi":"10.1177/2320206820960862","DOIUrl":"https://doi.org/10.1177/2320206820960862","url":null,"abstract":"Aim: Cancer stem cells (CSCs) or tumor-initiating cells have self-renewal and uncontrolled tumor growth capacity that promotes metastasis and recurrence. Challenges in anticancer have found a lateral dimension of treatment against a new pattern of tumor microcirculation, known as vasculogenic mimicry (VM), involved in cancer progression. Increasing evidence suggest that CSCs are involved in the formation of VM. In this review the correlation between CSCs and VM formation is been enlightened. Materials and Methods: The literature search was done in Medline, PubMed, Wiley, Science Direct, and Scopus. The keywords used for database search were cancer stem cells, vasculogenic mimicry, and anticancer therapy. Results: A total of 112 articles appeared from various sources, of which 102 were subjected for screening and 20 were related to the research objective. Conclusion: Based on the literature a positive correlation exists between CSC and VM, which plays a key role in tumor progression, and hence, can serve as a potential target in anticancer therapy.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"11 1","pages":"34 - 41"},"PeriodicalIF":0.7,"publicationDate":"2020-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78109581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-04DOI: 10.1177/2320206820953934
N. Yilmaz, Rukiye Yavaşer, A. A. Karagozler
Aim: To evaluate the therapeutic antioxidant effect of Hypericum perforatum L. (HPL) extract on oxidized dentin as well as to address the following research question: Is experimental HPL-derived antioxidant treatment protocol as effective as sodium ascorbate in terms of reversing the compromised resin bonding to bleached dentin? Materials and Methods: Eighty bovine permanent mandibular incisors were included in the shear bond strength (SBS) test and randomly assigned into four groups (n = 20) as follows: negative control (NC), positive control (PC), sodium ascorbate (SA), and Hypericum perforatum L. (HPL). Group NC did not receive bleaching treatment. All specimens except the NC group were oxidized by bleaching with 35% hydrogen peroxide. Experimental antioxidant solutions were prepared from SA powder and HPL extract at 10% concentrations. The bleached specimens in the SA and HPL groups were treated with respective antioxidant solutions. The SBS test was performed at a crosshead speed of 1 mm/min. The SBS data were analyzed with one-way analysis of variance using the post-hoc Bonferroni correction. The total phenolic content and the antioxidant activity of HPL extract were assessed by using the Folin–Ciocalteu assay and the 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging activity test, respectively. Results: Considering the SBS test results, statistically significant the highest and the lowest mean values were observed in NC and PC, respectively (P <.05). SA and HPL were not significantly different (P > .05). The total phenolic content (104 ± 1.740 mg gallic acid equivalents (GAE)/gram (GAE/g) dry HPL extract) and the antioxidant activity of the HPL extract were demonstrated. Conclusions: The natural HPL plant extract was as effective as SA in reversing the compromised resin bonding to bleached dentin. Antioxidant treatment derived from the HPL extract may enable immediate resin restoration of oxidized dentin.
{"title":"Hypericum perforatum L.: A Potent Antioxidant Source for the Treatment of Oxidized Dentin: An Experimental In Vitro Study","authors":"N. Yilmaz, Rukiye Yavaşer, A. A. Karagozler","doi":"10.1177/2320206820953934","DOIUrl":"https://doi.org/10.1177/2320206820953934","url":null,"abstract":"Aim: To evaluate the therapeutic antioxidant effect of Hypericum perforatum L. (HPL) extract on oxidized dentin as well as to address the following research question: Is experimental HPL-derived antioxidant treatment protocol as effective as sodium ascorbate in terms of reversing the compromised resin bonding to bleached dentin? Materials and Methods: Eighty bovine permanent mandibular incisors were included in the shear bond strength (SBS) test and randomly assigned into four groups (n = 20) as follows: negative control (NC), positive control (PC), sodium ascorbate (SA), and Hypericum perforatum L. (HPL). Group NC did not receive bleaching treatment. All specimens except the NC group were oxidized by bleaching with 35% hydrogen peroxide. Experimental antioxidant solutions were prepared from SA powder and HPL extract at 10% concentrations. The bleached specimens in the SA and HPL groups were treated with respective antioxidant solutions. The SBS test was performed at a crosshead speed of 1 mm/min. The SBS data were analyzed with one-way analysis of variance using the post-hoc Bonferroni correction. The total phenolic content and the antioxidant activity of HPL extract were assessed by using the Folin–Ciocalteu assay and the 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging activity test, respectively. Results: Considering the SBS test results, statistically significant the highest and the lowest mean values were observed in NC and PC, respectively (P <.05). SA and HPL were not significantly different (P > .05). The total phenolic content (104 ± 1.740 mg gallic acid equivalents (GAE)/gram (GAE/g) dry HPL extract) and the antioxidant activity of the HPL extract were demonstrated. Conclusions: The natural HPL plant extract was as effective as SA in reversing the compromised resin bonding to bleached dentin. Antioxidant treatment derived from the HPL extract may enable immediate resin restoration of oxidized dentin.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"79 1","pages":"57 - 65"},"PeriodicalIF":0.7,"publicationDate":"2020-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87945446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-04DOI: 10.1177/2320206820953965
Saloni Kachhara, D. Nallaswamy, D. Ganapathy, S. Maiti
Aim: To explore the evidence available to evaluate if simplified dentures could be as effective as the conventional complete dentures. Materials and Methods: A total of 1,482 articles were obtained using keywords in a Boolean search operator in the PubMed search engine, Google Scholar and Cochrane Library. Duplicates were removed and the remaining articles were subjected to a title analysis which yielded a total of 20. Further analysis of the article abstracts led to an exclusion of four articles. The remaining 16 articles were subjected to full text analysis which finally yielded a total of 11 articles. Results: The cumulative results of the meta-analysis for oral health-related quality of life, patient satisfaction and professional quality of denture show no significant difference (P > .05) between conventional and simplified dentures. Conclusion: Based on the available evidence adjusted for the potential literature/geographic bias, we conclude that the conventional procedures in complete denture fabrication such as facebow and gothic arch tracing do not improve the quality of the denture outcome from patients’ perspective and simplified dentures are equally effective as conventional complete dentures.
{"title":"A Comprehensive Systematic Review In Search Of Evidence for Pragmatic Altercations in Simplified Denture","authors":"Saloni Kachhara, D. Nallaswamy, D. Ganapathy, S. Maiti","doi":"10.1177/2320206820953965","DOIUrl":"https://doi.org/10.1177/2320206820953965","url":null,"abstract":"Aim: To explore the evidence available to evaluate if simplified dentures could be as effective as the conventional complete dentures. Materials and Methods: A total of 1,482 articles were obtained using keywords in a Boolean search operator in the PubMed search engine, Google Scholar and Cochrane Library. Duplicates were removed and the remaining articles were subjected to a title analysis which yielded a total of 20. Further analysis of the article abstracts led to an exclusion of four articles. The remaining 16 articles were subjected to full text analysis which finally yielded a total of 11 articles. Results: The cumulative results of the meta-analysis for oral health-related quality of life, patient satisfaction and professional quality of denture show no significant difference (P > .05) between conventional and simplified dentures. Conclusion: Based on the available evidence adjusted for the potential literature/geographic bias, we conclude that the conventional procedures in complete denture fabrication such as facebow and gothic arch tracing do not improve the quality of the denture outcome from patients’ perspective and simplified dentures are equally effective as conventional complete dentures.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"5 1","pages":"24 - 33"},"PeriodicalIF":0.7,"publicationDate":"2020-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80086260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.1177/2320206820949741
Pinjari Hameeda, S. Katti, Rajkishore Jammalamadugu, K. Bhatt, M. Peram, V. Kumbar
Aim: To evaluate and compare the effect of curcumin (CUR) and Nano-curcumin (N-CUR) on human-derived mesenchymal stem cells (MSCs) in a dose-dependent manner. Materials and Methods: An experimental study performed with putative MSCs from a total of five systemically healthy subjects with chronic periodontitis. These putative MSCs were isolated by cell culture and were further characterized and identified by colony-forming unit assay and immunocytochemical analysis using cell surface markers CD105, CD146, CD45 and CD73. The identified MSCs were treated with different doses of CUR and N-CUR, and compared with α-minimum essential medium (α-MEM) for its cell viability by performing MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay for 48 and 72 hr. The statistically analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test and Bonferroni’s post hoc test. Results: Compared to the α-MEM group, both CUR and N-CUR treated cells have shown significantly (P = .029) higher survival rate at lower concentration (0.1 and 0.5 µM/L), at 48 hr incubation. However, there was no statistically significant difference between the CUR and N-CUR groups on cell survival rate at both 48 and 72 hr incubation. When compared between the concentrations of the same group, significantly higher cell viability (P = .001) was observed at lower concentrations (0.1, 0.5 µM/L) in both test groups after incubation for 48 and 72 hr. Conclusion: Both CUR and N-CUR have a dose-dependent effect on human derived MSCs survival when incubated for 48 hr, whereas N-CUR shows increased cell survival rate even at 72 hr of incubation. Although, the cautious use of CUR and N-CUR at higher concentrations is recommended.
{"title":"Comparison of Effects of Curcumin and Nano-curcumin on the Survival of Human-Derived Mesenchymal Stem Cells: An Experimental Study","authors":"Pinjari Hameeda, S. Katti, Rajkishore Jammalamadugu, K. Bhatt, M. Peram, V. Kumbar","doi":"10.1177/2320206820949741","DOIUrl":"https://doi.org/10.1177/2320206820949741","url":null,"abstract":"Aim: To evaluate and compare the effect of curcumin (CUR) and Nano-curcumin (N-CUR) on human-derived mesenchymal stem cells (MSCs) in a dose-dependent manner. Materials and Methods: An experimental study performed with putative MSCs from a total of five systemically healthy subjects with chronic periodontitis. These putative MSCs were isolated by cell culture and were further characterized and identified by colony-forming unit assay and immunocytochemical analysis using cell surface markers CD105, CD146, CD45 and CD73. The identified MSCs were treated with different doses of CUR and N-CUR, and compared with α-minimum essential medium (α-MEM) for its cell viability by performing MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay for 48 and 72 hr. The statistically analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test and Bonferroni’s post hoc test. Results: Compared to the α-MEM group, both CUR and N-CUR treated cells have shown significantly (P = .029) higher survival rate at lower concentration (0.1 and 0.5 µM/L), at 48 hr incubation. However, there was no statistically significant difference between the CUR and N-CUR groups on cell survival rate at both 48 and 72 hr incubation. When compared between the concentrations of the same group, significantly higher cell viability (P = .001) was observed at lower concentrations (0.1, 0.5 µM/L) in both test groups after incubation for 48 and 72 hr. Conclusion: Both CUR and N-CUR have a dose-dependent effect on human derived MSCs survival when incubated for 48 hr, whereas N-CUR shows increased cell survival rate even at 72 hr of incubation. Although, the cautious use of CUR and N-CUR at higher concentrations is recommended.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"11 1","pages":"148 - 155"},"PeriodicalIF":0.7,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85683792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.1177/2320206820952303
S. Shah, D. Nallaswamy, D. Ganapathy
Aim: To assess if milled cobalt chromium (Co-Cr) alloy offers significantly better marginal accuracy than cast Co-Cr alloy for screw-retained long-span dental implant framework. Materials and Methods: A search PICO was formulated using suitable keywords and an electronic search was initiated. The databases of PubMed, Cochrane Library, Google Scholar and Embase were searched for related articles. Bibliographies of randomised control trials and reviews, identified in the electronic search, were analysed for studies published outside the electronically searched journals. Electronic search identified 26 studies. A total of 16 studies were eliminated after reading the abstracts. Out of the remaining 10 studies, 3 were eliminated based on the inclusion and exclusion criteria, and finally 7 studies were finalised for systematic review. Results Data were extracted from the included studies and analysed. The obtained data were suitable for meta-analysis, which showed an overall effect size z = 4.97 (P < .001) at 95% CI showing a significant statistical difference between milled and cast Co-Cr frameworks. Conclusion: Milled cobalt chromium frameworks are significantly more accurate than cast frameworks for long-span implant-supported frameworks used in dental prosthetic rehabilitation. More randomized controlled trials need to be conducted with a larger sample size to get a more authentic conclusion in a clinical scenario.
{"title":"Marginal Accuracy of Milled Versus Cast Cobalt Chromium Alloys in Long Span Implant-Supported Frameworks: A Systematic Review and Meta-analysis","authors":"S. Shah, D. Nallaswamy, D. Ganapathy","doi":"10.1177/2320206820952303","DOIUrl":"https://doi.org/10.1177/2320206820952303","url":null,"abstract":"Aim: To assess if milled cobalt chromium (Co-Cr) alloy offers significantly better marginal accuracy than cast Co-Cr alloy for screw-retained long-span dental implant framework. Materials and Methods: A search PICO was formulated using suitable keywords and an electronic search was initiated. The databases of PubMed, Cochrane Library, Google Scholar and Embase were searched for related articles. Bibliographies of randomised control trials and reviews, identified in the electronic search, were analysed for studies published outside the electronically searched journals. Electronic search identified 26 studies. A total of 16 studies were eliminated after reading the abstracts. Out of the remaining 10 studies, 3 were eliminated based on the inclusion and exclusion criteria, and finally 7 studies were finalised for systematic review. Results Data were extracted from the included studies and analysed. The obtained data were suitable for meta-analysis, which showed an overall effect size z = 4.97 (P < .001) at 95% CI showing a significant statistical difference between milled and cast Co-Cr frameworks. Conclusion: Milled cobalt chromium frameworks are significantly more accurate than cast frameworks for long-span implant-supported frameworks used in dental prosthetic rehabilitation. More randomized controlled trials need to be conducted with a larger sample size to get a more authentic conclusion in a clinical scenario.","PeriodicalId":43017,"journal":{"name":"Journal of Advanced Oral Research","volume":"47 1","pages":"120 - 127"},"PeriodicalIF":0.7,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85689285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-01DOI: 10.1177/2320206820942401
A. Ashour
Health deterioration at high altitudes is related to many pathophysiological processes. The literature contains much evidence describing the multiple factors responsible for this deterioration, including hypoxia, decreased oxygen saturation, and decreased barometric pressure. An increase in the levels of oxidative stress at high altitudes can lead to many serious health events, although the human body may be able to adapt to such changes in the case of people who are accustomed to living at a high altitude. The pathophysiological processes at high altitudes also have adverse effects on oral tissue health. The aim of this review is to summarize the major published findings about oral health deterioration of living in and travelling to high-altitude areas. Most studies have noted the adverse effects of prolonged exposure to hypoxia at high altitudes on oral tissues, including the salivary gland, periodontal tissue, gingival crevicular fluids, and jawbone. These changes include increases in the levels of inflammatory mediators and periodontal inflammation parameters. In addition, the incidence and severity of dental fluorosis is higher in residents of high-altitude areas. Abrupt changes in barometric pressure in people who travel to high altitudes may cause dental barotrauma and appear to increase sensitivity to pain (barodontalgia). In conclusion, it is important to consider that travelling to and living in high-altitude areas is a possible risk factor for multiple oral diseases.
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