Pub Date : 2019-06-01DOI: 10.19439/J.SJOS.2019.03.007
Ji Guo-ping, Tian Yi-hong, Liu Mei-xi, Shen Ying, S. Gang
PURPOSE: This study was aimed to figure out the way that cyclic-stretch influenced the apoptosis of myoblasts and evaluate the importance of PERK and its possible mechanism involved. METHODS: L6 rat myoblasts were cultured in vitro and mechanical stimulation model was constructed successfully. The myoblasts were imposed tension for 0, 2, 6, 12 and 24 hours respectively by multi-channel cell stress loading system. The force value was 15% cell deformation and the frequency was 10 cycles/min. Each cycle was consisted of stretch for 3 seconds and relaxation for 3 seconds, and the group without tension was used as the control group. The apoptotic myoblasts were dyed by DAPI and observed through fluorescence microscopy to detect the apoptosis rate; the mRNA levels of PERK and CHOP in different groups were detected by real-time PCR and protein levels of PERK and p-PERK in different groups were detected by Western blot. PERK inhibitor was used to clear the role of PERK in apoptosis induced by cyclic-stretch and clarify the relationship between the endoplasmic reticulum stress and apoptosis induced by cyclic-stretch. SPSS 17.0 software package was used to analyze the data statistically. RESULTS: DAPI nuclear stain showed that cyclical tensile stress can induce apoptosis in vitro cultured myoblast. Apoptosis rate showed a trend of rising gradually over time, peaked at 24 h. After dealt with the inhibitor of PERK, the apoptosis rate of the 24 h group under the cyclic stretch showed no difference compared with the control. The results of real- time PCR showed that the mRNA of CHOP was increased with the extension loading time, while the mRNA of PERK showed no difference compared with the control. Western blot results showed that the protein level of p-PERK was increased with the extension of loading time, while the expression of PERK showed no difference compared with the control group. When PERK inhibitor added, the mRNA level of CHOP along with the protein expression level of p-PERK showed no significant difference compared to the control. CONCLUSIONS: PERK signaling pathway is involved in the apoptosis of myoblasts induced by cyclic stretch, and the possible mechanism may be closely related to the phosphorylation of PERK.
{"title":"The role and mechanism of endoplasmic reticulum stress key factor PREK in myoblast apoptosis under stress loading","authors":"Ji Guo-ping, Tian Yi-hong, Liu Mei-xi, Shen Ying, S. Gang","doi":"10.19439/J.SJOS.2019.03.007","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.03.007","url":null,"abstract":"PURPOSE: This study was aimed to figure out the way that cyclic-stretch influenced the apoptosis of myoblasts and evaluate the importance of PERK and its possible mechanism involved. METHODS: L6 rat myoblasts were cultured in vitro and mechanical stimulation model was constructed successfully. The myoblasts were imposed tension for 0, 2, 6, 12 and 24 hours respectively by multi-channel cell stress loading system. The force value was 15% cell deformation and the frequency was 10 cycles/min. Each cycle was consisted of stretch for 3 seconds and relaxation for 3 seconds, and the group without tension was used as the control group. The apoptotic myoblasts were dyed by DAPI and observed through fluorescence microscopy to detect the apoptosis rate; the mRNA levels of PERK and CHOP in different groups were detected by real-time PCR and protein levels of PERK and p-PERK in different groups were detected by Western blot. PERK inhibitor was used to clear the role of PERK in apoptosis induced by cyclic-stretch and clarify the relationship between the endoplasmic reticulum stress and apoptosis induced by cyclic-stretch. SPSS 17.0 software package was used to analyze the data statistically. RESULTS: DAPI nuclear stain showed that cyclical tensile stress can induce apoptosis in vitro cultured myoblast. Apoptosis rate showed a trend of rising gradually over time, peaked at 24 h. After dealt with the inhibitor of PERK, the apoptosis rate of the 24 h group under the cyclic stretch showed no difference compared with the control. The results of real- time PCR showed that the mRNA of CHOP was increased with the extension loading time, while the mRNA of PERK showed no difference compared with the control. Western blot results showed that the protein level of p-PERK was increased with the extension of loading time, while the expression of PERK showed no difference compared with the control group. When PERK inhibitor added, the mRNA level of CHOP along with the protein expression level of p-PERK showed no significant difference compared to the control. CONCLUSIONS: PERK signaling pathway is involved in the apoptosis of myoblasts induced by cyclic stretch, and the possible mechanism may be closely related to the phosphorylation of PERK.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117184891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-25DOI: 10.19439/J.SJOS.2019.02.022
Jiang Wei, Xiaoxu Song, Y. Hui, Li Xiao-zhi
PURPOSE This study was to evaluate the upper airway changes in patients with skeletal Class Ⅲ maxillary retrognathia after rapid maxillary expansion and protraction. METHODS An electronic search in PubMed, Cochrane Library, Embase, CNKI, CBM, VIP and Wanfang was performed until March 1st, 2018. According to the inclusion and exclusion criteria, two investigators respectively reviewed the literature and selected eligible studies, then assessed the risks of bias and extracted the data of the included studies. The extracted data were quantitatively analyzed with Revman 5.3 software. RESULTS A total of 12 studies were included for meta-analysis. The results showed that, after treatment the nasopharyngeal volum(P 0.05) and hypopharyneal volume (P>0.05) existed. The nasopharyngeal airway dimensions had increased significantly with the following measurements: PNS-ad1 (P 0.05), IPS(P>0.05). CONCLUSIONS Rapid maxillary expansion and protraction can increase nasopharyngeal volume and sagittal airway dimensions in skeletal Class Ⅲ subjects with maxillary retrusion. It may be suggested that rapid maxillary expansion and protraction have the potential to reduce the risk of obstructive sleep apnea syndrome in children with maxillary retrusion by enlarging airway space.
{"title":"[Upper airway changes in patients with skeletal Class III maxillary retrognathia after rapid maxillary expansion and protraction: a meta-analysis].","authors":"Jiang Wei, Xiaoxu Song, Y. Hui, Li Xiao-zhi","doi":"10.19439/J.SJOS.2019.02.022","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.02.022","url":null,"abstract":"PURPOSE This study was to evaluate the upper airway changes in patients with skeletal Class Ⅲ maxillary retrognathia after rapid maxillary expansion and protraction. METHODS An electronic search in PubMed, Cochrane Library, Embase, CNKI, CBM, VIP and Wanfang was performed until March 1st, 2018. According to the inclusion and exclusion criteria, two investigators respectively reviewed the literature and selected eligible studies, then assessed the risks of bias and extracted the data of the included studies. The extracted data were quantitatively analyzed with Revman 5.3 software. RESULTS A total of 12 studies were included for meta-analysis. The results showed that, after treatment the nasopharyngeal volum(P 0.05) and hypopharyneal volume (P>0.05) existed. The nasopharyngeal airway dimensions had increased significantly with the following measurements: PNS-ad1 (P 0.05), IPS(P>0.05). CONCLUSIONS Rapid maxillary expansion and protraction can increase nasopharyngeal volume and sagittal airway dimensions in skeletal Class Ⅲ subjects with maxillary retrusion. It may be suggested that rapid maxillary expansion and protraction have the potential to reduce the risk of obstructive sleep apnea syndrome in children with maxillary retrusion by enlarging airway space.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124646549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-25DOI: 10.19439/J.SJOS.2018.06.001
Shen Gang
: Since its introduction, the diagnostic classification for malocclusions with facial prognathism has been well recognized and practically applied in orthodontic specialty. According to this classification, the malocclusions with a prognathic facial profile can be categorized into 4 classes: dento-alveolar originated, skeletally originated, mandibular positioning and combined skeletal and mandibular positioning, i.e, combined subtype I and subtype II. Among the new developments of this innovative malocclusion classification, the morphological assessment of mandibular body highlights a new impetus. Other than measuring the angular and linear perimeters only, this new dogma focuses on evaluating the physical contours of the mandible through cephalometric images. It includes the determination of geometric morphology of the mandible and the depth of symphysis curvature. Two types of mandibular morphology are common in patients with facial prognathism: a favorable flat and horizontally rectangular body with a prominent chin; and a unfavorable thick and vertically triangular body with a retrusive chin. The depth of the symphysis, on the other hand, is shown to be shallow or deep. It is noted that the prognathism with mandibular retruded positioning has a flat mandible and unfavorable triangle-shaped mandible exists only in skeletal prognathism. This new classification restricts the extraction indications for malocclusions with a prognathic facial profile: extraction should be refrained in cases with increased overjet, overbite and deep curve of Spee, and SGTB orthopedic therapy, instead, is recommended.
{"title":"Diagnostic classification for malocclusions with facial prognathism and its incorporation into mandibular morphological analysis","authors":"Shen Gang","doi":"10.19439/J.SJOS.2018.06.001","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.06.001","url":null,"abstract":": Since its introduction, the diagnostic classification for malocclusions with facial prognathism has been well recognized and practically applied in orthodontic specialty. According to this classification, the malocclusions with a prognathic facial profile can be categorized into 4 classes: dento-alveolar originated, skeletally originated, mandibular positioning and combined skeletal and mandibular positioning, i.e, combined subtype I and subtype II. Among the new developments of this innovative malocclusion classification, the morphological assessment of mandibular body highlights a new impetus. Other than measuring the angular and linear perimeters only, this new dogma focuses on evaluating the physical contours of the mandible through cephalometric images. It includes the determination of geometric morphology of the mandible and the depth of symphysis curvature. Two types of mandibular morphology are common in patients with facial prognathism: a favorable flat and horizontally rectangular body with a prominent chin; and a unfavorable thick and vertically triangular body with a retrusive chin. The depth of the symphysis, on the other hand, is shown to be shallow or deep. It is noted that the prognathism with mandibular retruded positioning has a flat mandible and unfavorable triangle-shaped mandible exists only in skeletal prognathism. This new classification restricts the extraction indications for malocclusions with a prognathic facial profile: extraction should be refrained in cases with increased overjet, overbite and deep curve of Spee, and SGTB orthopedic therapy, instead, is recommended.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123403002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-25DOI: 10.19439/J.SJOS.2018.06.002
郭延伟, 杨世茂
PURPOSE To investigate the feasibility of repairing damaged cartilage of rabbit condylar with TGF-β3-transfected adipose stem cells combined with OGP (osteogenesis peptide)-HA (hyaluronic acid)-ChS (chondroitin sulfate) scaffold. METHODS Rabbit ADSCs were isolated and cultured. The expression vector carrying TGF-β3 gene was constructed using recombinant adenovirus and transfected into rabbit ADSCs. The fluorescence expression of the cells was observed after 14 days and the efficiency of virus transfection was calculated. Western blot was used to detect the expression of TGF-β3 protein. Fifty rabbits were randomly divided into 5 groups: group A as blank group, group B as TGF-β3 transfected ADSCs group, group C as OGP-HA-ChS scaffold group, group D as ADSCs complex OGP-HA-ChS group, E group was TGF-β3 transfected ADSCs with OGP-HA-ChS. After the rabbit temporomandibular joint osteoarthrosis model was established, the animals were transplanted according to the experimental design. The animals in each group were sacrificed after the 3rd and 9th weeks of transplantation. Scanning electron microscopy, histological observation and real-time fluorescent quantitative PCR were performed accordingly. SPSS17.0 software package was used for analysis of variance and t test. RESULTS Scanning electron microscopy and histological observation showed that the repair of cartilage lesions in group D and E was better than those in group B and C. Compared with group B, the results of real-time quantitative PCR showed that the expression of MMP-3 in group E was similar to that in group A, but significantly lower than that in group C and D (P<0.05). The expression of TIMP-1 in group E was similar to that of group A, but significantly higher than group C and D (P<0.05). CONCLUSIONS TGF-β3 transfected ADSCs composite OGP-HA-ChS scaffold has repairing effect on rabbit condylar damaged cartilage.
{"title":"[Effect of TGF-β3 transfected with adipose derived stem cells and OGP-HA-chondroitin sulfate scaffold on repair of condylar cartilage in rabbits].","authors":"郭延伟, 杨世茂","doi":"10.19439/J.SJOS.2018.06.002","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.06.002","url":null,"abstract":"PURPOSE To investigate the feasibility of repairing damaged cartilage of rabbit condylar with TGF-β3-transfected adipose stem cells combined with OGP (osteogenesis peptide)-HA (hyaluronic acid)-ChS (chondroitin sulfate) scaffold. METHODS Rabbit ADSCs were isolated and cultured. The expression vector carrying TGF-β3 gene was constructed using recombinant adenovirus and transfected into rabbit ADSCs. The fluorescence expression of the cells was observed after 14 days and the efficiency of virus transfection was calculated. Western blot was used to detect the expression of TGF-β3 protein. Fifty rabbits were randomly divided into 5 groups: group A as blank group, group B as TGF-β3 transfected ADSCs group, group C as OGP-HA-ChS scaffold group, group D as ADSCs complex OGP-HA-ChS group, E group was TGF-β3 transfected ADSCs with OGP-HA-ChS. After the rabbit temporomandibular joint osteoarthrosis model was established, the animals were transplanted according to the experimental design. The animals in each group were sacrificed after the 3rd and 9th weeks of transplantation. Scanning electron microscopy, histological observation and real-time fluorescent quantitative PCR were performed accordingly. SPSS17.0 software package was used for analysis of variance and t test. RESULTS Scanning electron microscopy and histological observation showed that the repair of cartilage lesions in group D and E was better than those in group B and C. Compared with group B, the results of real-time quantitative PCR showed that the expression of MMP-3 in group E was similar to that in group A, but significantly lower than that in group C and D (P<0.05). The expression of TIMP-1 in group E was similar to that of group A, but significantly higher than group C and D (P<0.05). CONCLUSIONS TGF-β3 transfected ADSCs composite OGP-HA-ChS scaffold has repairing effect on rabbit condylar damaged cartilage.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"216 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115068591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.19439/J.SJOS.2018.06.005
Wang Yinghui, Qiu Fen-fang, P. Yu, Wu Wei-qing, Cheng Hui
PURPOSE To investigate the effects of recasting on metallographic microstructure of cobalt-chromium(Co-Cr), commercially pure titanium(cpTi), palladium-copper-gallium(Pd-Cu-Ga), aurum-platinum(Au-Pt) ceramic alloys. METHODS Without adding new alloys, Co-Cr, cpTi, Pd-Cu-Ga and Au-Pt ceramic alloys were recasted for 1-3 times under the circumstance of vacuum compressive casting with argon. Before each recasting, these 4 ceramic alloys casted previously were treated by the most appropriate way which has been reported by our previous study. According to GB/T13298-2015 standards, the specimens of 4 ceramic alloys after recasting 1-3 times were grinded, polished, and etched and then the metallographic microstructure of them were determined by a metallographic microscope or scanning electron microscopy(SEM). RESULTS With the increase of recasting times, the microstructure of Co-Cr ceramic alloy showed grain growth, grain matrix increase, and the intermetallic compound in the grain boundary increase, the microstructure of the cpTi ceramic alloy showed grain growth. The samples of cpTi ceramic alloy recasting twice appeared shaft tendency, and the ones which were recasted 3 times were compound of α-phase and β-phase. The microstructure of the Pd-Cu-Ga ceramic alloy showed grain growth slightly after casting 2-3 times, while no significant change was found on the microstructure of the Au-Pt ceramic alloy. CONCLUSIONS Recasting changes the microstructure of Co-Cr, cpTi, and Pd-Cu-Ga ceramic alloys, and the ceramic alloys may recycled by the manufacturers.
{"title":"The effect of recasting on microstructure of dental ceramic alloys","authors":"Wang Yinghui, Qiu Fen-fang, P. Yu, Wu Wei-qing, Cheng Hui","doi":"10.19439/J.SJOS.2018.06.005","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.06.005","url":null,"abstract":"PURPOSE To investigate the effects of recasting on metallographic microstructure of cobalt-chromium(Co-Cr), commercially pure titanium(cpTi), palladium-copper-gallium(Pd-Cu-Ga), aurum-platinum(Au-Pt) ceramic alloys. METHODS Without adding new alloys, Co-Cr, cpTi, Pd-Cu-Ga and Au-Pt ceramic alloys were recasted for 1-3 times under the circumstance of vacuum compressive casting with argon. Before each recasting, these 4 ceramic alloys casted previously were treated by the most appropriate way which has been reported by our previous study. According to GB/T13298-2015 standards, the specimens of 4 ceramic alloys after recasting 1-3 times were grinded, polished, and etched and then the metallographic microstructure of them were determined by a metallographic microscope or scanning electron microscopy(SEM). RESULTS With the increase of recasting times, the microstructure of Co-Cr ceramic alloy showed grain growth, grain matrix increase, and the intermetallic compound in the grain boundary increase, the microstructure of the cpTi ceramic alloy showed grain growth. The samples of cpTi ceramic alloy recasting twice appeared shaft tendency, and the ones which were recasted 3 times were compound of α-phase and β-phase. The microstructure of the Pd-Cu-Ga ceramic alloy showed grain growth slightly after casting 2-3 times, while no significant change was found on the microstructure of the Au-Pt ceramic alloy. CONCLUSIONS Recasting changes the microstructure of Co-Cr, cpTi, and Pd-Cu-Ga ceramic alloys, and the ceramic alloys may recycled by the manufacturers.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131200861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.19439/J.SJOS.2018.06.006
Gu Mei-zhen, Xu Hong-ming, C. Fang, Li Xiao-yan, Jia Yu-gang, Liu Ke-yong
PURPOSE The aim of this study was to provide basis for future design and selection of cleft palate surgery through establishing finite element model of pharyngeal cavity which was suitable for biomechanical analysis. METHODS One patient with isolated cleft palate and 1 normal child underwent multilayer head CT examination. The scanned data of pharyngeal cavity were imported into Mimics software for a 3-D geometric model reconstruction. The model was divided into a grid, so it can be further processed for subsequent finite element analysis. RESULTS After applying 5cm water column pressure load of 0.0005 MPa at the back edge of the soft palate in the two models respectively, the results showed that the maximum stress of the abnormal nasopharyngeal cavity model was 0.025 MPa, greater than the normal model (0.017 MPa). The same pressure loading was applied to different parts of the two models, the stress change area in the posterior margin of the soft palate and the middle of the palate was the same, and the stress in the front of the hard palate was smaller. CONCLUSIONS Finite element model has good biomechanical characteristics and geometric similarity. It can be used in isolated cleft palate with preoperative biomechanical analysis, for repairing and functional reconstructive surgery to provide ideal biomechanical model predicts.
{"title":"[Establishment of pharyngeal 3-dimensional finite element model of patients with isolated cleft palate].","authors":"Gu Mei-zhen, Xu Hong-ming, C. Fang, Li Xiao-yan, Jia Yu-gang, Liu Ke-yong","doi":"10.19439/J.SJOS.2018.06.006","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.06.006","url":null,"abstract":"PURPOSE The aim of this study was to provide basis for future design and selection of cleft palate surgery through establishing finite element model of pharyngeal cavity which was suitable for biomechanical analysis. METHODS One patient with isolated cleft palate and 1 normal child underwent multilayer head CT examination. The scanned data of pharyngeal cavity were imported into Mimics software for a 3-D geometric model reconstruction. The model was divided into a grid, so it can be further processed for subsequent finite element analysis. RESULTS After applying 5cm water column pressure load of 0.0005 MPa at the back edge of the soft palate in the two models respectively, the results showed that the maximum stress of the abnormal nasopharyngeal cavity model was 0.025 MPa, greater than the normal model (0.017 MPa). The same pressure loading was applied to different parts of the two models, the stress change area in the posterior margin of the soft palate and the middle of the palate was the same, and the stress in the front of the hard palate was smaller. CONCLUSIONS Finite element model has good biomechanical characteristics and geometric similarity. It can be used in isolated cleft palate with preoperative biomechanical analysis, for repairing and functional reconstructive surgery to provide ideal biomechanical model predicts.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133313478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-01DOI: 10.19439/J.SJOS.2018.04.019
Guo Biao, Lu Rong-jian, G. Cheng
PURPOSE To explore the effect of diode laser in the treatment of sinus chronic apical abscess. METHODS A total of 78 patients with sinus chronic apical abscess were randomly divided into experimental group and control group, 39 cases in each group. Patients in the control group were treated with conventional root canal preparation and calcium hydroxide sealant. After conventional root canal preparation, the canals in the experimental group were disinfected with diode laser and then calcium hydroxide sealant. SPSS 19.0 software package was used for data analysis. RESULTS Of 39 patients in the experimental group, only 1 sinus was not healed,the total effective rate was 97.44%;Of 39 patients in the control group,9 sinuses were not healed, the total effective rate was 76.92%. The difference between the two groups was statistically significant. CONCLUSIONS The results show that the effect of diode laser in the treatment of sinus chronic apical abscess is better, which is worthy of wide application.
{"title":"Effect of diode laser in the treatment of 78 patients with sinus chronic apical abscess","authors":"Guo Biao, Lu Rong-jian, G. Cheng","doi":"10.19439/J.SJOS.2018.04.019","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.04.019","url":null,"abstract":"PURPOSE To explore the effect of diode laser in the treatment of sinus chronic apical abscess. METHODS A total of 78 patients with sinus chronic apical abscess were randomly divided into experimental group and control group, 39 cases in each group. Patients in the control group were treated with conventional root canal preparation and calcium hydroxide sealant. After conventional root canal preparation, the canals in the experimental group were disinfected with diode laser and then calcium hydroxide sealant. SPSS 19.0 software package was used for data analysis. RESULTS Of 39 patients in the experimental group, only 1 sinus was not healed,the total effective rate was 97.44%;Of 39 patients in the control group,9 sinuses were not healed, the total effective rate was 76.92%. The difference between the two groups was statistically significant. CONCLUSIONS The results show that the effect of diode laser in the treatment of sinus chronic apical abscess is better, which is worthy of wide application.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127571330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-20DOI: 10.19439/J.SJOS.2018.03.010
W. Xi, Li Guang-hui, G. Jia, Sun Qiang
PURPOSE This study identified the differentially expressed miR-199b-5p in the progressing of head and neck squamous cell carcinoma(HNSCC) and investigated its biological characters. METHODS The expression of miR-199b-5p in 128 HNSCC tissues samples was evaluated. The association between clinicopathological parameters and the expression levels of the candidated miRNAs was analyzed by using Kaplan-Meier survival analysis. Cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells. Statistical analysis was performed using SPSS 19.0 software package. RESULTS In 79 HNSCC tissues with cervical lymph node metastasis, the expression level of miR-199b-5p was 1.68±0.21; while the expression level was 2.64±0.24 in 28 tissues without lymph node metastasis (P=0.001). In patients with HNSCC, lower level of miR-199b-5p expression significantly correlated with worse overall survival rate (P=0.01). Overexpression of miR-199b-5p inhibited HNSCC cell proliferation and invasion. CONCLUSIONS miR-199b-5p plays a key role in cell invasion and metastasis and its expression correlated with overall survival in patients with HNSCC.
{"title":"miR-199b-5p inhibits proliferation and invasion of head and neck cancer cells","authors":"W. Xi, Li Guang-hui, G. Jia, Sun Qiang","doi":"10.19439/J.SJOS.2018.03.010","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.03.010","url":null,"abstract":"PURPOSE This study identified the differentially expressed miR-199b-5p in the progressing of head and neck squamous cell carcinoma(HNSCC) and investigated its biological characters. METHODS The expression of miR-199b-5p in 128 HNSCC tissues samples was evaluated. The association between clinicopathological parameters and the expression levels of the candidated miRNAs was analyzed by using Kaplan-Meier survival analysis. Cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells. Statistical analysis was performed using SPSS 19.0 software package. RESULTS In 79 HNSCC tissues with cervical lymph node metastasis, the expression level of miR-199b-5p was 1.68±0.21; while the expression level was 2.64±0.24 in 28 tissues without lymph node metastasis (P=0.001). In patients with HNSCC, lower level of miR-199b-5p expression significantly correlated with worse overall survival rate (P=0.01). Overexpression of miR-199b-5p inhibited HNSCC cell proliferation and invasion. CONCLUSIONS miR-199b-5p plays a key role in cell invasion and metastasis and its expression correlated with overall survival in patients with HNSCC.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129669176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-20DOI: 10.19439/j.sjos.2018.03.022
Xu Xiao-bo, Hou Yong-fu, Bian Hua-qin, W. Mian, Huang Yan.
{"title":"[Effect of full crown preparation with ultrasound instrument on marginal adaption: a randomized controlled trial].","authors":"Xu Xiao-bo, Hou Yong-fu, Bian Hua-qin, W. Mian, Huang Yan.","doi":"10.19439/j.sjos.2018.03.022","DOIUrl":"https://doi.org/10.19439/j.sjos.2018.03.022","url":null,"abstract":"","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128409636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.19439/J.SJOS.2018.03.009
X. Bing, H. Tao, H. Xin
PURPOSE To investigation the effect of flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells. METHODS After evaluation of the toxic effect of chrysin, preosteoblast MC3T3-E1 cell suspension was prepared with DMEM and then the cells were cultured with 0 and 25 μmol/L chrysin. Real-time quantitative PCR was used to detect the expression of osteogenic marlcers including Runx2, ColA1 and OCN. Statistical analysis was performed using SPSS17.0 software package. RESULTS 25 μmol/L chrysin had no toxic effect on preosteoblast MC3T3-E1 cells. After co-culture with 25 μmol/L chrysin,the expression of Runx2, ColA1 and OCN was highest at the 7th,14th and 21th day,respectively. CONCLUSIONS 25 μmol/L chrysin can promote osteogenic gene expression of preosteoblast MC3T3-E1 cell.
{"title":"[Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells].","authors":"X. Bing, H. Tao, H. Xin","doi":"10.19439/J.SJOS.2018.03.009","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.03.009","url":null,"abstract":"PURPOSE To investigation the effect of flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells. METHODS After evaluation of the toxic effect of chrysin, preosteoblast MC3T3-E1 cell suspension was prepared with DMEM and then the cells were cultured with 0 and 25 μmol/L chrysin. Real-time quantitative PCR was used to detect the expression of osteogenic marlcers including Runx2, ColA1 and OCN. Statistical analysis was performed using SPSS17.0 software package. RESULTS 25 μmol/L chrysin had no toxic effect on preosteoblast MC3T3-E1 cells. After co-culture with 25 μmol/L chrysin,the expression of Runx2, ColA1 and OCN was highest at the 7th,14th and 21th day,respectively. CONCLUSIONS 25 μmol/L chrysin can promote osteogenic gene expression of preosteoblast MC3T3-E1 cell.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123025939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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