Pub Date : 2015-12-28DOI: 10.20538/1682-0363-2015-4-60-67
M. Y. Rosensteyn, A. Z. Rosensteyn, S. E. Kondakov, N. A. Cherevko
A new methodological approach to analysis and interpretation of data from a multifaceted analysis ELISA IgG has been provided. The possibility for identification of a “norma-pathology” criterion, on the basis of research yielding a frequency spectrum (or probability density function-PDF) of IgG-immune responses in the integral IgG-immune reply is shown. The possibility for unified diagnostic of hypersensi-tivity type III in patients with varying levels of food disadaptation and symptoms of pathological reac-tions to food antigens is shown too.
{"title":"НОВЫЙ МЕТОДОЛОГИЧЕСКИЙ ПОДХОД К СОЗДАНИЮ ПЕРСОНИФИЦИРОВАННОЙ ЭЛИМИНАЦИОННОЙ ДИЕТЫ ПРИ ПИЩЕВОЙ НЕПЕРЕНОСИМОСТИ, ОБУСЛОВЛЕННОЙ ИММУНОПАТОЛОГИЧЕСКИМИ РЕАКЦИЯМИ III ТИПА","authors":"M. Y. Rosensteyn, A. Z. Rosensteyn, S. E. Kondakov, N. A. Cherevko","doi":"10.20538/1682-0363-2015-4-60-67","DOIUrl":"https://doi.org/10.20538/1682-0363-2015-4-60-67","url":null,"abstract":"A new methodological approach to analysis and interpretation of data from a multifaceted analysis ELISA IgG has been provided. The possibility for identification of a “norma-pathology” criterion, on the basis of research yielding a frequency spectrum (or probability density function-PDF) of IgG-immune responses in the integral IgG-immune reply is shown. The possibility for unified diagnostic of hypersensi-tivity type III in patients with varying levels of food disadaptation and symptoms of pathological reac-tions to food antigens is shown too.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"14 1","pages":"60-67"},"PeriodicalIF":0.2,"publicationDate":"2015-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-28DOI: 10.20538/1682-0363-2015-6-61-67
O. L. Nosareva, Ye. A. Stepovaya, N. V. Ryazantseva, Ye. V. Shakhristova, O. N. Vesnina, V. V. Novitsky
The formation of oxidative stress lies at the heart of many frequent and socially-important diseases. Blood lymphocytes are the cells which provide immunological control of our organism. As a result of their function implementation blood lymphocytes contact with different endogenic and exogenic factors, which can lead to active oxygen species production activation, macromolecules oxidative modification and to cell survival alteration. At the present time it is essential to expand and deepen the fundamental knowledge of blood lymphocytes apoptosis regulation peculiarities. The research objective was to establish the interaction among alterations of glutathione system condition, carbonylation level, protein glutathionylation and caspase-3 activity in blood lymphocytes during oxidative stress in vitro. Material and Methods. The material for research was blood lymphocytes cultivated with addition of hydrogen peroxide in final concentration of 0,5 mmol and/or protein SH-group inhibitor N- ethylmaleimide – 5 mmol, protector – 5 mmol – 1,4-dithioerythritol. Reduced, oxidized and protein-bound glutathione concentration was measured by method of spectropho-tometry, additionally, the ratio size of reduced to oxidized thiol fraction was estimated. With help of enzymoimmunoassay the level of protein carbonyl derivatives was evaluated; caspase-3 activity was registered by spectrofluorometric method. Results . Protein SH-group blocking in blood lymphocytes during oxidative stress in vitro was accompanied by protein-bound glutathione concentration rapid decrease in connection with increase of protein carbonyl derivatives content and caspase-3 activity. Protein SH-group protection in blood lymphocytes during oxidative stress in vitro was accompanied by concentration increase of protein-bound glutathione and protein carbonyl derivatives under comparable values of enzyme activity under study. Conclusion . The carried out research shows that caspase-3 and protein-bound glutathione are the molecular targets of selective control over programmed cell death. The received indices of caspase-3 activity change and protein-bound glutathione concentration alteration in blood lymphocytes during oxidative stress in vitro can be used when elaborating target therapy approaches to diseases accompanied by apoptosis disregulation.
{"title":"РОЛЬ ОКИСЛИТЕЛЬНОЙ МОДИФИКАЦИИ БЕЛКОВ В РЕДОКС-РЕГУЛЯЦИИ АКТИВНОСТИ КАСПАЗЫ-3 В ЛИМФОЦИТАХ КРОВИ ПРИ ОКИСЛИТЕЛЬНОМ СТРЕССЕ IN VITRO","authors":"O. L. Nosareva, Ye. A. Stepovaya, N. V. Ryazantseva, Ye. V. Shakhristova, O. N. Vesnina, V. V. Novitsky","doi":"10.20538/1682-0363-2015-6-61-67","DOIUrl":"https://doi.org/10.20538/1682-0363-2015-6-61-67","url":null,"abstract":"The formation of oxidative stress lies at the heart of many frequent and socially-important diseases. Blood lymphocytes are the cells which provide immunological control of our organism. As a result of their function implementation blood lymphocytes contact with different endogenic and exogenic factors, which can lead to active oxygen species production activation, macromolecules oxidative modification and to cell survival alteration. At the present time it is essential to expand and deepen the fundamental knowledge of blood lymphocytes apoptosis regulation peculiarities. The research objective was to establish the interaction among alterations of glutathione system condition, carbonylation level, protein glutathionylation and caspase-3 activity in blood lymphocytes during oxidative stress in vitro. Material and Methods. The material for research was blood lymphocytes cultivated with addition of hydrogen peroxide in final concentration of 0,5 mmol and/or protein SH-group inhibitor N- ethylmaleimide – 5 mmol, protector – 5 mmol – 1,4-dithioerythritol. Reduced, oxidized and protein-bound glutathione concentration was measured by method of spectropho-tometry, additionally, the ratio size of reduced to oxidized thiol fraction was estimated. With help of enzymoimmunoassay the level of protein carbonyl derivatives was evaluated; caspase-3 activity was registered by spectrofluorometric method. Results . Protein SH-group blocking in blood lymphocytes during oxidative stress in vitro was accompanied by protein-bound glutathione concentration rapid decrease in connection with increase of protein carbonyl derivatives content and caspase-3 activity. Protein SH-group protection in blood lymphocytes during oxidative stress in vitro was accompanied by concentration increase of protein-bound glutathione and protein carbonyl derivatives under comparable values of enzyme activity under study. Conclusion . The carried out research shows that caspase-3 and protein-bound glutathione are the molecular targets of selective control over programmed cell death. The received indices of caspase-3 activity change and protein-bound glutathione concentration alteration in blood lymphocytes during oxidative stress in vitro can be used when elaborating target therapy approaches to diseases accompanied by apoptosis disregulation.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"14 1","pages":"61-67"},"PeriodicalIF":0.2,"publicationDate":"2015-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-26DOI: 10.20538/1682-0363-2015-5-15-21
G. A. Galkina, A. A. Voropay, M. Levkovich, S. V. Vorobiov, M. V. Komkova, N. V. Morozova
This study was aimed to determine peculiarities in regimens of the pump insulin therapy and to reveal the optimal basal-to-bolus insulin ratio that are necessary for achieving optimal glycemic control in adoles-cents with type 1 diabetes mellitus (T1DM). 82 adolescents at the age of 14–18 with T1DM, using continuous subcutaneous insulin infusion (CSII) from 5 months to 7.5 years were monitored with continuous glucose monitoring (CGM) system «Guar-dian Real Time» or CGM system, built in MiniMed Paradigm Revel System 722 (Medtronic Minimed, USA). Assessing the quality of glycaemic control was based on the level of glycated haemoglobin (HbA1c). The results of CGM were reviewed and average for 3 days performances: total daily dose of insulin, dose of basal and bolus insulin, basal-to-bolus insulin ratio, carbohydrate content of the meal, expressed in BE, carbohydrate ratio, insulin sensitivity factor were determined. The patients were subdivided into 2 groups: group 1 – adolescents with the optimal/suboptimal glycemic control ( n = 55), 2 – adolescents with long-standing poorly controlled T1DM ( n = 27). Average total daily dose of basal insulin (U in a day, U per kg in a day) in adolescents group 1 was significantly higher, com-pared with patients in group 2 ( р = 0.043; р = 0.038 respectively). Patients in group 2 received more car-bohydrates with a meal intake and had higher doses of average total daily bolus insulin. The average ba-sal-to-bolus ratio from group 1 patients was 51/49%, compared with group 2 patients – 45/55% ( р = 0.026). An important condition for achieving optimal glycemic control is a high level of compliance and skills of adolescents. Optimal well-balanced basal-to-bolus insulin ratio in adolescents with T1DM on CSII, which can provide improvements in blood glucose management and reducing the risk of complications of the disease, is 51/49%.
本研究旨在确定泵胰岛素治疗方案的特殊性，并揭示在1型糖尿病(T1DM)青少年中实现最佳血糖控制所必需的最佳基础与剂量胰岛素比。82名14-18岁的T1DM青少年，连续皮下胰岛素输注(CSII) 5个月至7.5年，使用连续血糖监测(CGM)系统“guardian -dian Real Time”或CGM系统进行监测，该系统采用MiniMed Paradigm Revel system 722(美顿力MiniMed，美国)。评估血糖控制的质量是基于糖化血红蛋白(HbA1c)的水平。对CGM试验结果进行回顾，并对3天的性能进行平均:测定每日胰岛素总剂量、基础胰岛素和单剂量胰岛素、基础胰岛素与单剂量胰岛素的比值、膳食碳水化合物含量、BE表达、碳水化合物比值、胰岛素敏感因子。患者再分为2组:1组-血糖控制最佳/次优的青少年(n = 55)， 2组-长期控制不良的T1DM青少年(n = 27)。青少年组1的日均基础胰岛素总剂量(U in a day, U per kg in a day)显著高于组2 (r = 0.043;分别为0.038)。第二组患者在用餐时摄入更多的碳水化合物，并且每天平均总胰岛素剂量更高。1组患者的平均盐丸比为51/49%，而2组患者为45/55% (χ = 0.026)。实现最佳血糖控制的一个重要条件是青少年的高水平依从性和技能。经CSII治疗的T1DM青少年的最佳平衡基础胰岛素与胰岛素比为51/49%，这可以改善血糖管理并降低疾病并发症的风险。
{"title":"Оптимальные режимы базис-болюсной инсулинотерапии у подростков с сахарным диабетом 1-го типа","authors":"G. A. Galkina, A. A. Voropay, M. Levkovich, S. V. Vorobiov, M. V. Komkova, N. V. Morozova","doi":"10.20538/1682-0363-2015-5-15-21","DOIUrl":"https://doi.org/10.20538/1682-0363-2015-5-15-21","url":null,"abstract":"This study was aimed to determine peculiarities in regimens of the pump insulin therapy and to reveal the optimal basal-to-bolus insulin ratio that are necessary for achieving optimal glycemic control in adoles-cents with type 1 diabetes mellitus (T1DM). 82 adolescents at the age of 14–18 with T1DM, using continuous subcutaneous insulin infusion (CSII) from 5 months to 7.5 years were monitored with continuous glucose monitoring (CGM) system «Guar-dian Real Time» or CGM system, built in MiniMed Paradigm Revel System 722 (Medtronic Minimed, USA). Assessing the quality of glycaemic control was based on the level of glycated haemoglobin (HbA1c). The results of CGM were reviewed and average for 3 days performances: total daily dose of insulin, dose of basal and bolus insulin, basal-to-bolus insulin ratio, carbohydrate content of the meal, expressed in BE, carbohydrate ratio, insulin sensitivity factor were determined. The patients were subdivided into 2 groups: group 1 – adolescents with the optimal/suboptimal glycemic control ( n = 55), 2 – adolescents with long-standing poorly controlled T1DM ( n = 27). Average total daily dose of basal insulin (U in a day, U per kg in a day) in adolescents group 1 was significantly higher, com-pared with patients in group 2 ( р = 0.043; р = 0.038 respectively). Patients in group 2 received more car-bohydrates with a meal intake and had higher doses of average total daily bolus insulin. The average ba-sal-to-bolus ratio from group 1 patients was 51/49%, compared with group 2 patients – 45/55% ( р = 0.026). An important condition for achieving optimal glycemic control is a high level of compliance and skills of adolescents. Optimal well-balanced basal-to-bolus insulin ratio in adolescents with T1DM on CSII, which can provide improvements in blood glucose management and reducing the risk of complications of the disease, is 51/49%.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"25 1","pages":"15-21"},"PeriodicalIF":0.2,"publicationDate":"2015-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-24DOI: 10.20538/1682-0363-2015-1-81-91
Татьяна Владимировна Саприна, Т. С. Прохоренко, Наталья Владимировна Рязанцева, Ирина Николаевна Ворожцова, С Э Мартынова, Анна Николаевна Дзюман, Анастасия Павловна Зима, О. С. Попов, Игорь Альбертович Хлусов, Карине Тимуровна Касоян, Ирина Петровна Шабалова
Very little research is devoted to the study of communication systemic immunological changes and local immunological changes in the tissue of the thyroid in autoimmune thyrophaties. The goal of the research was to establish immunologic and morphologic predictors of clinical course and functional outcomes of Graves’s disease (GD). Material and methods. We examined 45 patients with GD (14 men and 31 women) aged 18–55 years (47.0 (35.0–53.0)) years, matching the following criteria: a verified diagnosis GD, accommodation in Tomsk oblast and the Tomsk not less than 10 years, the patient's consent for the study. The control group included 30 people, with an average age of (45.3 ± 5.6) years and was comparable by age and sex with GD patients. The study included: definition of the standard for GD hormonal and serological profile (free T4, free T3, and TSH, antibodies to TPO, antibodies to the TSH receptor), the cultivation of mononuclear leukocytes in complete culture medium within 24 hours with subsequent determination of interleukins concentrations (IL-2, IL-4, TNF-alpha) in culture medium using enzyme-linked immunosorbent assay, determination of the number of blood cells carrying membrane-bound receptors to IL-2, IL-4, TNF-R1 by flow laser cytometry on cytometer BD FACS Canto tmΙΙ (USA) using labeled monoclonal antibody, the standard postoperative histological examination of thyroid gland tissue samples and immunohistochemical detection in samples of thyroid tissue receptors to IL-2, IL-4, TNF-alpha. Results. On the basis of the received results it is possible to allocate 2 clinical-immunologic and morphologic cluster of autoimmune hyperthyroidism syndrome in patients with clinical diagnosis of GD. The first includes the formation of 1 and 2 histological options GD (minimally expressed monocytes infiltration, lack of oncocytic transformation of thyroid epithelium), with some clinical characteristics (persistent and pronounced hyperthyroidism, the large size of goiter, higher titer of receptor TSH-antibody and smaller TPO-antibody, diffuse nature of the lesion), and the second – is represented by 3d histological option with oncocytic restructuring of follicular epithelium, expressed the monocyte/macrophage infiltration with the TNF-RI expression and clinical patterns, including: the older age group of patients, the smaller size of goiter, the emergence of “pseudo nodes” ultrasound, reflecting the presence of lymphoid follicles-infiltrates, more lenient for hyperthyroidism. Probably, that the 2nd cluster is a combination of “classical GD” with autoimmune thyroiditis, however, a set of clinical and laboratory-instrumental signs led to the fact that these patients were in the group of GD patients. Conclusion. This approach (combining study of indicators of systemic and local tissue-specific autoimmune inflammation) is a promising from the point of view of separate parts coverage in the autoimmune thyroids diseases pathogenesis, approximating researchers t
{"title":"ИММУНОЛОГИЧЕСКИЕ И МОРФОЛОГИЧЕСКИЕ ПРЕДИКТОРЫ КЛИНИЧЕСКОЙ ГЕТЕРОГЕННОСТИ ПАЦИЕНТОВ С БОЛЕЗНЬЮ ГРЕЙВСА (ПО РЕЗУЛЬТАТАМ ИССЛЕДОВАНИЯ ОПЕРАТИВНОГО МАТЕРИАЛА ЩИТОВИДНОЙ ЖЕЛЕЗЫ)","authors":"Татьяна Владимировна Саприна, Т. С. Прохоренко, Наталья Владимировна Рязанцева, Ирина Николаевна Ворожцова, С Э Мартынова, Анна Николаевна Дзюман, Анастасия Павловна Зима, О. С. Попов, Игорь Альбертович Хлусов, Карине Тимуровна Касоян, Ирина Петровна Шабалова","doi":"10.20538/1682-0363-2015-1-81-91","DOIUrl":"https://doi.org/10.20538/1682-0363-2015-1-81-91","url":null,"abstract":"Very little research is devoted to the study of communication systemic immunological changes and local immunological changes in the tissue of the thyroid in autoimmune thyrophaties. The goal of the research was to establish immunologic and morphologic predictors of clinical course and functional outcomes of Graves’s disease (GD). Material and methods. We examined 45 patients with GD (14 men and 31 women) aged 18–55 years (47.0 (35.0–53.0)) years, matching the following criteria: a verified diagnosis GD, accommodation in Tomsk oblast and the Tomsk not less than 10 years, the patient's consent for the study. The control group included 30 people, with an average age of (45.3 ± 5.6) years and was comparable by age and sex with GD patients. The study included: definition of the standard for GD hormonal and serological profile (free T4, free T3, and TSH, antibodies to TPO, antibodies to the TSH receptor), the cultivation of mononuclear leukocytes in complete culture medium within 24 hours with subsequent determination of interleukins concentrations (IL-2, IL-4, TNF-alpha) in culture medium using enzyme-linked immunosorbent assay, determination of the number of blood cells carrying membrane-bound receptors to IL-2, IL-4, TNF-R1 by flow laser cytometry on cytometer BD FACS Canto tmΙΙ (USA) using labeled monoclonal antibody, the standard postoperative histological examination of thyroid gland tissue samples and immunohistochemical detection in samples of thyroid tissue receptors to IL-2, IL-4, TNF-alpha. Results. On the basis of the received results it is possible to allocate 2 clinical-immunologic and morphologic cluster of autoimmune hyperthyroidism syndrome in patients with clinical diagnosis of GD. The first includes the formation of 1 and 2 histological options GD (minimally expressed monocytes infiltration, lack of oncocytic transformation of thyroid epithelium), with some clinical characteristics (persistent and pronounced hyperthyroidism, the large size of goiter, higher titer of receptor TSH-antibody and smaller TPO-antibody, diffuse nature of the lesion), and the second – is represented by 3d histological option with oncocytic restructuring of follicular epithelium, expressed the monocyte/macrophage infiltration with the TNF-RI expression and clinical patterns, including: the older age group of patients, the smaller size of goiter, the emergence of “pseudo nodes” ultrasound, reflecting the presence of lymphoid follicles-infiltrates, more lenient for hyperthyroidism. Probably, that the 2nd cluster is a combination of “classical GD” with autoimmune thyroiditis, however, a set of clinical and laboratory-instrumental signs led to the fact that these patients were in the group of GD patients. Conclusion. This approach (combining study of indicators of systemic and local tissue-specific autoimmune inflammation) is a promising from the point of view of separate parts coverage in the autoimmune thyroids diseases pathogenesis, approximating researchers t","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"14 1","pages":"81-91"},"PeriodicalIF":0.2,"publicationDate":"2015-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-24DOI: 10.20538/1682-0363-2014-6-57-71
В. В. Калюжин, Александр Трофимович Тепляков, Инна Давидовна Беспалова, Е. В. Калюжина
The authors of the review have analyzed papers published on the problem of ischemic myocardial dysfunction. They begin with a definition of the term “ischemia” (derived from two Greek words: isch ō , meaning to hold back, and haima, meaning blood) - a condition at which the arterial blood flow is insufficient to provide enough oxygen to prevent intracellular respiration from shifting from the aerobic to the anaerobic form. The poor rate of ATP generation from this process causes a decrease in cellular ATP, a concomitant rise in ADP, and ultimately, to depression inotropic (systolic) and lusitropic (diastolic) function of the affected segments of the myocardium. But with such simplicity of basic concepts, the consequences of ischemia so diverse. Influence of an ischemia on myocardial function so unequally at different patients, which is almost impossible to find two identical cases (as in the case of fingerprints). It depends on the infinite variety of lesions of coronary arteries, reperfusion (time and completeness of restoration of blood flow) and reactions of a myocardium which, apparently, has considerable flexibility in its response. Ischemic myocardial dysfunction includes a number of discrete states, such as acute left ventricular failure in angina, acute myocardial infarction, ischemic cardiomyopathy, stunning, hibernation, pre- and postconditioning. There are widely differing underlying pathophysiologic states. The possibility exists that several of these states can coexist.
{"title":"К ВОПРОСУ ОБ ИШЕМИЧЕСКОЙ ДИСФУНКЦИИ МИОКАРДА","authors":"В. В. Калюжин, Александр Трофимович Тепляков, Инна Давидовна Беспалова, Е. В. Калюжина","doi":"10.20538/1682-0363-2014-6-57-71","DOIUrl":"https://doi.org/10.20538/1682-0363-2014-6-57-71","url":null,"abstract":"The authors of the review have analyzed papers published on the problem of ischemic myocardial dysfunction. They begin with a definition of the term “ischemia” (derived from two Greek words: isch ō , meaning to hold back, and haima, meaning blood) - a condition at which the arterial blood flow is insufficient to provide enough oxygen to prevent intracellular respiration from shifting from the aerobic to the anaerobic form. The poor rate of ATP generation from this process causes a decrease in cellular ATP, a concomitant rise in ADP, and ultimately, to depression inotropic (systolic) and lusitropic (diastolic) function of the affected segments of the myocardium. But with such simplicity of basic concepts, the consequences of ischemia so diverse. Influence of an ischemia on myocardial function so unequally at different patients, which is almost impossible to find two identical cases (as in the case of fingerprints). It depends on the infinite variety of lesions of coronary arteries, reperfusion (time and completeness of restoration of blood flow) and reactions of a myocardium which, apparently, has considerable flexibility in its response. Ischemic myocardial dysfunction includes a number of discrete states, such as acute left ventricular failure in angina, acute myocardial infarction, ischemic cardiomyopathy, stunning, hibernation, pre- and postconditioning. There are widely differing underlying pathophysiologic states. The possibility exists that several of these states can coexist.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"13 1","pages":"57-71"},"PeriodicalIF":0.2,"publicationDate":"2015-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-24DOI: 10.20538/1682-0363-2015-1-126-134
S. A. Moreva, A. V. Silaeva, A. N. Kornetov, Ye. N. Borodina, A. Y. Yankovskaya, N. I. Pryadukhina, N. E. Golovakha, V. B. Obukhovskaya
The article presents a social-psychological indicators of stress, coping mechanisms, rigidity and psychological component gestational dominant as risk factors predisposing to deviations in the context of preparedness for motherhood, marked psychological characteristics of pregnant women main group. Material and methods. The study was conducted on the basis of obstetric clinics SSMU, prenatal maternity hospital number 4 and the Centre for Medical Prevention (Cabinet crisis pregnancy). Total was attended by 52 women aged 18 to 44 years, at different stages of pregnancy – from 5 to 39 weeks. Methodological apparatus consisted of a structured interview questionnaire “Medical and social data of the pregnant woman” methodology for determining the stress and social adaptation of Holmes and Rage (1967), a questionnaire of coping R. Lazarus and S. Folkman (WCQ, 1988), Tomsk questionnaire rigidity G.V. Zalewski (TORZ, 1987), test on pregnant I.V. Dobryakova (1996). Data processing was carried out using statistical methods Spearman rank correlation coefficient, nonparametric Mann–Whitney U and Kruskal–Wallis H and φ * – Fisher's angular transformation. Results. Significant psychological factors that may influence the formation of readiness for motherhood among women of the main group can be considered an unplanned pregnancy (65%), and also its not gelendost (35%). Studies of stress resistance was detected in pregnant women from the main group low stress levels (88%), and only 12% have a high degree. Most of the women from the сomparison group reverse the situation 64% have a high degree of stress, this suggests that the presence of previously state miscarriage is for this group of stress. On the formation of readiness for motherhood affects not only the presence of stressful situations, butalso how the woman in their lives. Using U-test Mann–Whitney, were statistically significant differences were observed among the main group and сomparison group in the following coping strategies: selfcontrol (p ≤ 0.0014), the search for social support (p ≤ 0.0015), accepting responsibility (p ≤ 0.04), and planning solutions to the problems (p ≤ 0.00016). These differences are considered as one of the risk factors for deviant motherhood main group. In contrast to the сomparison group, where 62% of pregnant women with the optimal type PKGD, main group optimal type PKGD observed only in 38% of the respondents, which indicates that the risk of deviations in the structure of psychological readiness. Conclusion. Risk factors such as social disadvantage, the planned abandonment of a child, the tendency to rigid behavior, inefficient use of coping strategies and low level of optimal psychological component gestational dominant testify about the possible psychological unpreparedness for the upcoming event to become a mother and as a consequence to the development of deviant motherhood.
{"title":"Психологические механизмы формирования девиаций в период подготовки к материнству","authors":"S. A. Moreva, A. V. Silaeva, A. N. Kornetov, Ye. N. Borodina, A. Y. Yankovskaya, N. I. Pryadukhina, N. E. Golovakha, V. B. Obukhovskaya","doi":"10.20538/1682-0363-2015-1-126-134","DOIUrl":"https://doi.org/10.20538/1682-0363-2015-1-126-134","url":null,"abstract":"The article presents a social-psychological indicators of stress, coping mechanisms, rigidity and psychological component gestational dominant as risk factors predisposing to deviations in the context of preparedness for motherhood, marked psychological characteristics of pregnant women main group. Material and methods. The study was conducted on the basis of obstetric clinics SSMU, prenatal maternity hospital number 4 and the Centre for Medical Prevention (Cabinet crisis pregnancy). Total was attended by 52 women aged 18 to 44 years, at different stages of pregnancy – from 5 to 39 weeks. Methodological apparatus consisted of a structured interview questionnaire “Medical and social data of the pregnant woman” methodology for determining the stress and social adaptation of Holmes and Rage (1967), a questionnaire of coping R. Lazarus and S. Folkman (WCQ, 1988), Tomsk questionnaire rigidity G.V. Zalewski (TORZ, 1987), test on pregnant I.V. Dobryakova (1996). Data processing was carried out using statistical methods Spearman rank correlation coefficient, nonparametric Mann–Whitney U and Kruskal–Wallis H and φ * – Fisher's angular transformation. Results. Significant psychological factors that may influence the formation of readiness for motherhood among women of the main group can be considered an unplanned pregnancy (65%), and also its not gelendost (35%). Studies of stress resistance was detected in pregnant women from the main group low stress levels (88%), and only 12% have a high degree. Most of the women from the сomparison group reverse the situation 64% have a high degree of stress, this suggests that the presence of previously state miscarriage is for this group of stress. On the formation of readiness for motherhood affects not only the presence of stressful situations, butalso how the woman in their lives. Using U-test Mann–Whitney, were statistically significant differences were observed among the main group and сomparison group in the following coping strategies: selfcontrol (p ≤ 0.0014), the search for social support (p ≤ 0.0015), accepting responsibility (p ≤ 0.04), and planning solutions to the problems (p ≤ 0.00016). These differences are considered as one of the risk factors for deviant motherhood main group. In contrast to the сomparison group, where 62% of pregnant women with the optimal type PKGD, main group optimal type PKGD observed only in 38% of the respondents, which indicates that the risk of deviations in the structure of psychological readiness. Conclusion. Risk factors such as social disadvantage, the planned abandonment of a child, the tendency to rigid behavior, inefficient use of coping strategies and low level of optimal psychological component gestational dominant testify about the possible psychological unpreparedness for the upcoming event to become a mother and as a consequence to the development of deviant motherhood.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"14 1","pages":"126-134"},"PeriodicalIF":0.2,"publicationDate":"2015-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-20DOI: 10.20538/1682-0363-2014-3-32-39
Виктор Владимирович Иванов, Е. В. Шахристова, Е. А. Степовая, О. Л. Носарева, Т. С. Фёдорова, Наталья Владимировна Рязанцева, Вячеслав Викторович Новицкий
Oxidative stress is one of the pathogenetic components of many diseases during which generation of reactive oxigen species increases and the capacity of the antioxidant protection system diminishes. In the research of the last decades special attention has been given to adipose tissue, production of adipokines by it and their role in development of immunoresistance associated with formation of the metabolic syndrome and diabetes. Search for methods of therapeutic correction of adipokine secretion disorders, their influence on metabolism of separate cells and the organism on the whole as well as development of new approaches to correction of disorders in cell sensitivity to insulin are extremely topical nowadays. Systematization and consolidation of accumulated data allow to determine the strategies of further research more accurately; as a result, we have attempted to summarize and analyze the accumulated data on the role of adipose tissue in oxidative stress development. On the basis of literature data and the results of the personal investigations, the role of adipose tissue in forming oxidative stress in diabetes has been analyzed in the article. Brief description of adipose tissue was given as a secretory organ regulating metabolic processes in adipocytes and influencing functions of various organs and systems of the body. Mechanisms of disorder in insulin secretion as well as development of insulin sesistance in type I diabetes were described along with the contribution of lipolysis in adipose tissue to these processes.
{"title":"Окислительный стресс: влияние на секрецию инсулина, рецепцию гормона адипоцитами и липолиз в жировой ткани","authors":"Виктор Владимирович Иванов, Е. В. Шахристова, Е. А. Степовая, О. Л. Носарева, Т. С. Фёдорова, Наталья Владимировна Рязанцева, Вячеслав Викторович Новицкий","doi":"10.20538/1682-0363-2014-3-32-39","DOIUrl":"https://doi.org/10.20538/1682-0363-2014-3-32-39","url":null,"abstract":"Oxidative stress is one of the pathogenetic components of many diseases during which generation of reactive oxigen species increases and the capacity of the antioxidant protection system diminishes. In the research of the last decades special attention has been given to adipose tissue, production of adipokines by it and their role in development of immunoresistance associated with formation of the metabolic syndrome and diabetes. Search for methods of therapeutic correction of adipokine secretion disorders, their influence on metabolism of separate cells and the organism on the whole as well as development of new approaches to correction of disorders in cell sensitivity to insulin are extremely topical nowadays. Systematization and consolidation of accumulated data allow to determine the strategies of further research more accurately; as a result, we have attempted to summarize and analyze the accumulated data on the role of adipose tissue in oxidative stress development. On the basis of literature data and the results of the personal investigations, the role of adipose tissue in forming oxidative stress in diabetes has been analyzed in the article. Brief description of adipose tissue was given as a secretory organ regulating metabolic processes in adipocytes and influencing functions of various organs and systems of the body. Mechanisms of disorder in insulin secretion as well as development of insulin sesistance in type I diabetes were described along with the contribution of lipolysis in adipose tissue to these processes.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"15 1","pages":"32-39"},"PeriodicalIF":0.2,"publicationDate":"2015-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-20DOI: 10.20538/1682-0363-2014-3-126-131
Нелли Борисовна Найговзина, А. К. Конаныхина, А. В. Кочубей, Э. В. Зимина, М. В. Наваркин
Implement approach of innovative education is development priorities of Russian system of training and continuous professional development of health professionals. Conception of innovative education is aligned with national policy, including statutory regulation, in the sphere of professional medical education.
{"title":"ПРИОРИТЕТНЫЕ ПУТИ РАЗВИТИЯ СИСТЕМЫ ПОДГОТОВКИ И НЕПРЕРЫВНОГО ПРОФЕССИОНАЛЬНОГО РАЗВИТИЯ КАДРОВ В СФЕРЕ ЗДРАВООХРАНЕНИЯ","authors":"Нелли Борисовна Найговзина, А. К. Конаныхина, А. В. Кочубей, Э. В. Зимина, М. В. Наваркин","doi":"10.20538/1682-0363-2014-3-126-131","DOIUrl":"https://doi.org/10.20538/1682-0363-2014-3-126-131","url":null,"abstract":"Implement approach of innovative education is development priorities of Russian system of training and continuous professional development of health professionals. Conception of innovative education is aligned with national policy, including statutory regulation, in the sphere of professional medical education.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"13 1","pages":"126-131"},"PeriodicalIF":0.2,"publicationDate":"2015-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-19DOI: 10.20538/1682-0363-2014-3-5-24
Н. А. Корнетов
Study objectives are dedicated to brief synthesized establishment of diagnostics general standard, management and therapy of major depressive disorders (MDD) in clinical medicine to provide education in medical practice. Study methods are based on short-term, medium-term and long-term educational programs in 24 Russian and Ukraine cities, which were based on original educational programs under the direction of World Psychiatric Association (WPA) and International Committee For Prevention and Treatment of Depression (PTD). There, about 1450 doctors of different occupations were acquainted with the detection, management and treatment of MDD. The Russian version of WPA/PTD programs was created. The program of education included 4 modules. The Core module included an overview of the epidemiology, impact, concepts and classification, and etiology of depressive disorders as well as their recognition, diagnosis, and management in the primary care setting. The second module focused on depressive disorders in physical illness and covered those major illnesses for which is reasonable evidence for an association with depressive disorders. The third module included the development of the depressive disorders in older persons. The forth module included training physicians in mental health skills. 39 theme improvements for 858 primary care setting physicians within the framework of faculty training program of physicians’ development were the part of other programs of physicians’ occupations. Results. A number of highlights were included in the general algorithm of the educational programs. The creation of optimum «physician-depressive patient» contact demands a number of new skills to establish effective communication. These skills involve training of meeting of depressive patient and physician, the ability to follow a certain communication style; identify emotional, cognitive, psychomotor and nonverbal patterns of behavior. Besides that, the education included adherence to therapy and therapeutic alliance during the period of informed agreement. There was an education of fluent grasp of medicaments, which are the first choice in therapy. Integration of this knowledge is done during the periods of repeated interactive discussions of antidepressants’ effectiveness in depression therapy which is based on three-phased MDD therapy model. The process of working out the depressive patients’ management was based on systematization and diagnostic of depression disorders (Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, or International Classification of Diseases, Revision 10); detailed discussions of manifestation and thorough depression symptomatology; management approaches in depression disorders, different in manifestation. Conclusion. The basis of physicians' education was the idea that depressive disorder in holistic approach can't be considered as an independent psychiatric disease in its mild and moderate forms of manifestation. Depression
{"title":"Семиотика, диагностика и тактика ведения терапии депрессивного расстройства в клинической медицине","authors":"Н. А. Корнетов","doi":"10.20538/1682-0363-2014-3-5-24","DOIUrl":"https://doi.org/10.20538/1682-0363-2014-3-5-24","url":null,"abstract":"Study objectives are dedicated to brief synthesized establishment of diagnostics general standard, management and therapy of major depressive disorders (MDD) in clinical medicine to provide education in medical practice. Study methods are based on short-term, medium-term and long-term educational programs in 24 Russian and Ukraine cities, which were based on original educational programs under the direction of World Psychiatric Association (WPA) and International Committee For Prevention and Treatment of Depression (PTD). There, about 1450 doctors of different occupations were acquainted with the detection, management and treatment of MDD. The Russian version of WPA/PTD programs was created. The program of education included 4 modules. The Core module included an overview of the epidemiology, impact, concepts and classification, and etiology of depressive disorders as well as their recognition, diagnosis, and management in the primary care setting. The second module focused on depressive disorders in physical illness and covered those major illnesses for which is reasonable evidence for an association with depressive disorders. The third module included the development of the depressive disorders in older persons. The forth module included training physicians in mental health skills. 39 theme improvements for 858 primary care setting physicians within the framework of faculty training program of physicians’ development were the part of other programs of physicians’ occupations. Results. A number of highlights were included in the general algorithm of the educational programs. The creation of optimum «physician-depressive patient» contact demands a number of new skills to establish effective communication. These skills involve training of meeting of depressive patient and physician, the ability to follow a certain communication style; identify emotional, cognitive, psychomotor and nonverbal patterns of behavior. Besides that, the education included adherence to therapy and therapeutic alliance during the period of informed agreement. There was an education of fluent grasp of medicaments, which are the first choice in therapy. Integration of this knowledge is done during the periods of repeated interactive discussions of antidepressants’ effectiveness in depression therapy which is based on three-phased MDD therapy model. The process of working out the depressive patients’ management was based on systematization and diagnostic of depression disorders (Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, or International Classification of Diseases, Revision 10); detailed discussions of manifestation and thorough depression symptomatology; management approaches in depression disorders, different in manifestation. Conclusion. The basis of physicians' education was the idea that depressive disorder in holistic approach can't be considered as an independent psychiatric disease in its mild and moderate forms of manifestation. Depression","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"13 1","pages":"5-24"},"PeriodicalIF":0.2,"publicationDate":"2015-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-08-28DOI: 10.20538/1682-0363-2015-4-82-86
G. P. Filippov, K. V. Boronenko, I. V. Plotnikova, Ye. S. Sitkova
Objective. To evaluate the main indicators characterizing the rigidity of the vascular wall in healthy ado-lescents with such risk factors (RF) for arterial hypertension (AH) as a family history on hypertension and smoking. Identify changes in the initial elastic-elastic properties of the arteries at the preclinical stage of development of hypertension. Material and methods. It was formed two groups of comparison. Age studied from 13 to 17 years (mean age (15.00 ± 0.31) years). The first group consisted of 30 healthy adolescents whose parents suffer from hypertension from a young age. The second group consisted of 30 healthy smokers teenager from healthy parents. The control group consisted of 30 healthy adolescents from healthy parents. Determines the basic stiffness of the vascular wall: PWV, CAVI, SAI. Results. A significant in crease in the indicators characterizing the rigidity of the vascular wall in the two comparison groups relative to the control. PWV: 6,89 ± 0,56 (first group), 7.13 ± 0.55 (second group) and 5.5 ± 0.41 (control), p < 0.05.L-CAVI: 5,46 ± 0,39 (first group), 5.84 ± 0.61 (second group) and 4.32 ± 0.41 (control), p < 0.05.R-CAVI: 5,63 ± 0,39 (first group), 5.89 ± 0.56 (second group) and 4.49 ± 0.41(control), p < 0.05. R-AI: 0,89 ± 0,09 (first group), 0.95 ± 0.12 (second group) and 0.62 ± 0.1 (control), p < 0.05. Smoking teenagers and adolescents with family history of hypertension, there are changes in the initial stiffness of the vessel wall, which requires the allocation of at-riskfor the development of hypertension and prevention activities at the preclinical stage of development ofthe disease.
{"title":"Неинвазивная оценка жесткости сосудистой стенки у здоровых подростков, имеющих факторы риска развития артериальной гипертензии","authors":"G. P. Filippov, K. V. Boronenko, I. V. Plotnikova, Ye. S. Sitkova","doi":"10.20538/1682-0363-2015-4-82-86","DOIUrl":"https://doi.org/10.20538/1682-0363-2015-4-82-86","url":null,"abstract":"Objective. To evaluate the main indicators characterizing the rigidity of the vascular wall in healthy ado-lescents with such risk factors (RF) for arterial hypertension (AH) as a family history on hypertension and smoking. Identify changes in the initial elastic-elastic properties of the arteries at the preclinical stage of development of hypertension. Material and methods. It was formed two groups of comparison. Age studied from 13 to 17 years (mean age (15.00 ± 0.31) years). The first group consisted of 30 healthy adolescents whose parents suffer from hypertension from a young age. The second group consisted of 30 healthy smokers teenager from healthy parents. The control group consisted of 30 healthy adolescents from healthy parents. Determines the basic stiffness of the vascular wall: PWV, CAVI, SAI. Results. A significant in crease in the indicators characterizing the rigidity of the vascular wall in the two comparison groups relative to the control. PWV: 6,89 ± 0,56 (first group), 7.13 ± 0.55 (second group) and 5.5 ± 0.41 (control), p < 0.05.L-CAVI: 5,46 ± 0,39 (first group), 5.84 ± 0.61 (second group) and 4.32 ± 0.41 (control), p < 0.05.R-CAVI: 5,63 ± 0,39 (first group), 5.89 ± 0.56 (second group) and 4.49 ± 0.41(control), p < 0.05. R-AI: 0,89 ± 0,09 (first group), 0.95 ± 0.12 (second group) and 0.62 ± 0.1 (control), p < 0.05. Smoking teenagers and adolescents with family history of hypertension, there are changes in the initial stiffness of the vessel wall, which requires the allocation of at-riskfor the development of hypertension and prevention activities at the preclinical stage of development ofthe disease.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":"14 1","pages":"82-86"},"PeriodicalIF":0.2,"publicationDate":"2015-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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