Obesity increases the risk of developing various pathological conditions such as metabolic syndrome, type 2 diabetes, non-alcoholic fatty liver disease, and cardiovascular disease. For mild correction and even treatment of obesity, drugs based on medicinal plants are increasingly being used. This work aims to determine the overall effect of Helichrysum arenarium inflorescences on physiological activity and metabolic processes in model animals fed a diet rich in fat and carbohydrates. In a 27-day experiment on 18 laboratory rats fed a diet with high-fat content and 20% fructose solution, we determined the effect of 0.4% and 4.0% dry inflorescences of H. arenarium from the feed mass on the animals' physiological activity and metabolic processes. The body weight of rats in the control group reached 121.7% of the initial body weight; when consuming 0.4% and 4.0% H. arenarium in the diet, this indicator was lower – 109.6% and 111.2%, respectively. Dry inflorescences of H. arenarium in the rats’ diet at a dose of 0.4% caused a decrease in the relative mass of the heart and thymus and an increase in the relative mass of the caecum and colon. A diet with the addition of H. arenarium caused a change in blood biochemical parameters: an increase in the urea concentration and urea nitrogen, and a decrease in the glucose concentration and protein coefficient. Helichrysum arenarium at both concentrations significantly increased the gamma-glutamyltransferase and alkaline phosphatase activity with a decrease in alpha-amylase activity in the blood. A 0.4% dose of inflorescences of the food mass caused a decrease in AST activity, and 200 g sharply increased blood ALT activity. The consumption of dried H. arenarium inflorescences decreased hematocrit, haemoglobin concentration, erythrocyte and platelet counts, and increased the percentage of eosinophils and monocytes. A decrease in physical activity and an intensification of emotional state were found in the animals after eating dry inflorescences of H. arenarium, regardless of the dose compared with the control group of animals. Significant changes in the orientation activity of the animals were not observed. The obtained results show that adding dry H. arenarium inflorescences as a food supplement to a high-calorie diet is safe, does not cause pathological changes and side effects, and significantly affects metabolic processes. This provides theoretical support for the use of dried H. arenarium inflorescences for the production of nutraceutical and pharmacological products for the correction of metabolic disorders in humans and animals. The doses and duration of their use require further research.
{"title":"The effect on the organism of rats of adding Helichrysum arenarium inflorescences to a hypercaloric diet, high in sugar and fat","authors":"M. A. Lieshchova, V. V. Brygadyrenko","doi":"10.15421/012341","DOIUrl":"https://doi.org/10.15421/012341","url":null,"abstract":"Obesity increases the risk of developing various pathological conditions such as metabolic syndrome, type 2 diabetes, non-alcoholic fatty liver disease, and cardiovascular disease. For mild correction and even treatment of obesity, drugs based on medicinal plants are increasingly being used. This work aims to determine the overall effect of Helichrysum arenarium inflorescences on physiological activity and metabolic processes in model animals fed a diet rich in fat and carbohydrates. In a 27-day experiment on 18 laboratory rats fed a diet with high-fat content and 20% fructose solution, we determined the effect of 0.4% and 4.0% dry inflorescences of H. arenarium from the feed mass on the animals' physiological activity and metabolic processes. The body weight of rats in the control group reached 121.7% of the initial body weight; when consuming 0.4% and 4.0% H. arenarium in the diet, this indicator was lower – 109.6% and 111.2%, respectively. Dry inflorescences of H. arenarium in the rats’ diet at a dose of 0.4% caused a decrease in the relative mass of the heart and thymus and an increase in the relative mass of the caecum and colon. A diet with the addition of H. arenarium caused a change in blood biochemical parameters: an increase in the urea concentration and urea nitrogen, and a decrease in the glucose concentration and protein coefficient. Helichrysum arenarium at both concentrations significantly increased the gamma-glutamyltransferase and alkaline phosphatase activity with a decrease in alpha-amylase activity in the blood. A 0.4% dose of inflorescences of the food mass caused a decrease in AST activity, and 200 g sharply increased blood ALT activity. The consumption of dried H. arenarium inflorescences decreased hematocrit, haemoglobin concentration, erythrocyte and platelet counts, and increased the percentage of eosinophils and monocytes. A decrease in physical activity and an intensification of emotional state were found in the animals after eating dry inflorescences of H. arenarium, regardless of the dose compared with the control group of animals. Significant changes in the orientation activity of the animals were not observed. The obtained results show that adding dry H. arenarium inflorescences as a food supplement to a high-calorie diet is safe, does not cause pathological changes and side effects, and significantly affects metabolic processes. This provides theoretical support for the use of dried H. arenarium inflorescences for the production of nutraceutical and pharmacological products for the correction of metabolic disorders in humans and animals. The doses and duration of their use require further research.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136044466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Global climate change provide the emergence of new opportunities for the introduction of new crops into horticultural production in the areas of insufficient precipitation. In addition to the economic aspect, it is also of interest to the biologists of the development of this plant in a qualitatively new environment.The paper considers the variability of the main traits of plant morphometry, yield for four varieties of hazelnuts in order to identify the most promising forms for cultivating in the northern part of the Steppe of Ukraine characterized by an insufficient precipitation and harsh winters. Recent milder winter conditions and a certain balance in summer droughts have made the required horticultural production possible, thereby increasing the production of hazelnuts and addressing the dietary problems of people in terms of supply of necessary vitamins and micronutrient element from hazelnuts. Promising varieties for the production plantations have been specified, the mechanisms for the yield formation have been studied. Key traits of morphometry have been identified that condition the success of a variety under insufficient humidity. These were such traits as crown volume, leaf surface area, shell thickness, average weight of one nut, weight of dry nuts, yield, kernel yield. It has been established that the yield formation on account of large, well-shaped nuts is best in terms of yield. Some aspects of yield formation and the possibility of combining different varieties, especially when more intensive growing methods are applied, are of additional interest. High variability significantly prevents the modeling of traits, for example, such as yield per tree, from being significant in terms of the formation of high yield. Semi-intensive pruning of hazelnut bushes shows its suitability for use in modern garden plantings. Variety Barselonskiy showed extremely high variability for many key parameters, which may indicate insufficient stability of this variety from a genetic point of view and the presence of a fairly significant number of hidden biotypes, which is additionally negative for cultivation in modern semi-intensive and intensive technologies. Climate change makes it possible to continue to significantly expand the area under hazelnuts due to the previously considered unfavourable southern subzones of the Forest-Steppe and the Steppe zone of Ukraine. Such new and previously unnoticed effects have been noted as fruiting in the first year in production crops, the formation of up to 5–6 inflorescences for each nut-bearing branch in the second or third year. For further investigations, it is planned to analyze the nutritional qualities of the obtained products, in terms of the composition by microelements and the presence of biologically-active substances, to trace the dynamics of the accumulation of heavy metals and the potentially associated risks.
{"title":"Diversity of hazelnut varieties and changes in plant development during introduction in the semi-arid zone","authors":"M. Nazarenko, O. Simchenko","doi":"10.15421/012336","DOIUrl":"https://doi.org/10.15421/012336","url":null,"abstract":"Global climate change provide the emergence of new opportunities for the introduction of new crops into horticultural production in the areas of insufficient precipitation. In addition to the economic aspect, it is also of interest to the biologists of the development of this plant in a qualitatively new environment.The paper considers the variability of the main traits of plant morphometry, yield for four varieties of hazelnuts in order to identify the most promising forms for cultivating in the northern part of the Steppe of Ukraine characterized by an insufficient precipitation and harsh winters. Recent milder winter conditions and a certain balance in summer droughts have made the required horticultural production possible, thereby increasing the production of hazelnuts and addressing the dietary problems of people in terms of supply of necessary vitamins and micronutrient element from hazelnuts. Promising varieties for the production plantations have been specified, the mechanisms for the yield formation have been studied. Key traits of morphometry have been identified that condition the success of a variety under insufficient humidity. These were such traits as crown volume, leaf surface area, shell thickness, average weight of one nut, weight of dry nuts, yield, kernel yield. It has been established that the yield formation on account of large, well-shaped nuts is best in terms of yield. Some aspects of yield formation and the possibility of combining different varieties, especially when more intensive growing methods are applied, are of additional interest. High variability significantly prevents the modeling of traits, for example, such as yield per tree, from being significant in terms of the formation of high yield. Semi-intensive pruning of hazelnut bushes shows its suitability for use in modern garden plantings. Variety Barselonskiy showed extremely high variability for many key parameters, which may indicate insufficient stability of this variety from a genetic point of view and the presence of a fairly significant number of hidden biotypes, which is additionally negative for cultivation in modern semi-intensive and intensive technologies. Climate change makes it possible to continue to significantly expand the area under hazelnuts due to the previously considered unfavourable southern subzones of the Forest-Steppe and the Steppe zone of Ukraine. Such new and previously unnoticed effects have been noted as fruiting in the first year in production crops, the formation of up to 5–6 inflorescences for each nut-bearing branch in the second or third year. For further investigations, it is planned to analyze the nutritional qualities of the obtained products, in terms of the composition by microelements and the presence of biologically-active substances, to trace the dynamics of the accumulation of heavy metals and the potentially associated risks.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135359619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Posidonia oceanica is an endemic species of the Mediterranean Sea that in pristine coastal waters forms extensive meadows, which are, actually, exposed to natural and anthropogenic pressures, causing their regression throughout the basin. The aim of our study is to summarise and evaluate current knowledge about the characterisation of Posidonia oceanica along the Western Algerian coast, in order to provide new evidence about the distribution and health status this species. To realize our objectives, we combined extracted data from published articles, theses, results of project reports, in situ observations and laboratory analysis. The results show the presence of the meadows in fifteen coastal zones of Western Algeria, mainly located in shallow surface waters from 0.5 to 18 metres depth.The mean leaf length across all sites ranged from 153 to 667 mm. The mean number of leaves/shoot ranged between 4.7 ± 0.9 and 6.0 ± 0.5 leaves/shoot. Leaf length showed a highly significant difference between the cold and hot season. The data analysis shows that the leaf biometric values and the shoot densities of the P. oceanica are greater than those of Mediterranean samples although these measures tend to decrease deeply. The analyses carried out on structural features of P. oceanica show that the Western Algerian meadows are, globally, in good health status according to standardised scale. The available data on P. oceanica summarised in this article represent an important starting point to build effective plans for understanding levels of environmental threats and for supporting conservation strategies for these important ecosystems. Conversely, the limited information available on this seagrass along the Western Algerian costs only allows the description of some structural features, and permits us to draw overall conclusions on its general health status.
{"title":"Distribution and status of Posidonia oceanica meadows on the North-Western coast of Algeria","authors":"M. E. A. Bentaallah, D. Baghdadi, N. Cantasano","doi":"10.15421/012328","DOIUrl":"https://doi.org/10.15421/012328","url":null,"abstract":"Posidonia oceanica is an endemic species of the Mediterranean Sea that in pristine coastal waters forms extensive meadows, which are, actually, exposed to natural and anthropogenic pressures, causing their regression throughout the basin. The aim of our study is to summarise and evaluate current knowledge about the characterisation of Posidonia oceanica along the Western Algerian coast, in order to provide new evidence about the distribution and health status this species. To realize our objectives, we combined extracted data from published articles, theses, results of project reports, in situ observations and laboratory analysis. The results show the presence of the meadows in fifteen coastal zones of Western Algeria, mainly located in shallow surface waters from 0.5 to 18 metres depth.The mean leaf length across all sites ranged from 153 to 667 mm. The mean number of leaves/shoot ranged between 4.7 ± 0.9 and 6.0 ± 0.5 leaves/shoot. Leaf length showed a highly significant difference between the cold and hot season. The data analysis shows that the leaf biometric values and the shoot densities of the P. oceanica are greater than those of Mediterranean samples although these measures tend to decrease deeply. The analyses carried out on structural features of P. oceanica show that the Western Algerian meadows are, globally, in good health status according to standardised scale. The available data on P. oceanica summarised in this article represent an important starting point to build effective plans for understanding levels of environmental threats and for supporting conservation strategies for these important ecosystems. Conversely, the limited information available on this seagrass along the Western Algerian costs only allows the description of some structural features, and permits us to draw overall conclusions on its general health status.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135755548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The legume-rhizobium interaction induces formation of specific reactions that take metabolism in the host plant up to a new functional level, increasing its tolerance to unfavourable cultivation conditions. Our objective was to study the participation of key enzymes – phenylalanine ammonia lyase, guaiacol peroxidase, and polyphenol oxidases – in the phenol-metabolism processes and synthesis of a broad spectrum of secondary metabolites in soybean plants that have established symbiotic interactions with rhizobia of varying effectiveness during optimal and insufficient water supplies. In our studies, we used symbiotic systems of soybean and rhizobia (Bradyrhizobium japonicum) that varied in efficiency and virulence. In the period of active nitrogen fixation by soybean, from the third-true-leaf stage until budding, we created different water-supply regimes for the plants, including optimal watering at the level of 60% of full field capacity (control) and insufficient, at the level of 30% (drought). When the soybean was flowering, we recovered the optimal level of water supply (resumed watering). In the studies, we employed microbiological, biochemical, and physiological approaches. We determined the specificity of how key enzymes of the phenol metabolism such as phenylalanine ammonia lyase, polyphenol oxidase and guaiacol peroxidase in the nodules, roots, and leaves of the soybean reacted to different levels of water supply, depending on the functional efficiency of the symbiotic system involving strains of B. japonicum, varying in effectiveness and virulence. In the effective soybean-rhizobium symbiosis, there occurred insignificant changes in the activity of phenol-metabolism enzymes in the nodules, roots, and leaves during drought and after action of the stress. This evidence is that in symbiosis with effective rhizobia B1-20, soybean could realize its own defensive systems that regulate optimal functioning of phenol metabolism in dehydration conditions. In the low-effective 107 and ineffective 604k symbiotic systems of soybean, there was observed unstable dynamics of the activity of enzymes in leaves and roots, manifested in intensification or inhibition of their activity levels during drought or post-stress period. This indicates malfunctioning of the processes associated with phenol metabolism in the soybean plants. We concluded that tolerance of legume-rhizobium symbiosis to water deprivation depends on mutual involvements of the both symbiotic partners – host plant and rhizobia, their ability to fully realize the defensive systems for activation of the key enzymatic complexes taking part in regulation of phenol metabolism in plants.
{"title":"Key role of phenol enzymes metabolism in the legume-rhizobial symbiosis under different water supply regimes","authors":"T. Nyzhnyk, S. Kots","doi":"10.15421/012335","DOIUrl":"https://doi.org/10.15421/012335","url":null,"abstract":"The legume-rhizobium interaction induces formation of specific reactions that take metabolism in the host plant up to a new functional level, increasing its tolerance to unfavourable cultivation conditions. Our objective was to study the participation of key enzymes – phenylalanine ammonia lyase, guaiacol peroxidase, and polyphenol oxidases – in the phenol-metabolism processes and synthesis of a broad spectrum of secondary metabolites in soybean plants that have established symbiotic interactions with rhizobia of varying effectiveness during optimal and insufficient water supplies. In our studies, we used symbiotic systems of soybean and rhizobia (Bradyrhizobium japonicum) that varied in efficiency and virulence. In the period of active nitrogen fixation by soybean, from the third-true-leaf stage until budding, we created different water-supply regimes for the plants, including optimal watering at the level of 60% of full field capacity (control) and insufficient, at the level of 30% (drought). When the soybean was flowering, we recovered the optimal level of water supply (resumed watering). In the studies, we employed microbiological, biochemical, and physiological approaches. We determined the specificity of how key enzymes of the phenol metabolism such as phenylalanine ammonia lyase, polyphenol oxidase and guaiacol peroxidase in the nodules, roots, and leaves of the soybean reacted to different levels of water supply, depending on the functional efficiency of the symbiotic system involving strains of B. japonicum, varying in effectiveness and virulence. In the effective soybean-rhizobium symbiosis, there occurred insignificant changes in the activity of phenol-metabolism enzymes in the nodules, roots, and leaves during drought and after action of the stress. This evidence is that in symbiosis with effective rhizobia B1-20, soybean could realize its own defensive systems that regulate optimal functioning of phenol metabolism in dehydration conditions. In the low-effective 107 and ineffective 604k symbiotic systems of soybean, there was observed unstable dynamics of the activity of enzymes in leaves and roots, manifested in intensification or inhibition of their activity levels during drought or post-stress period. This indicates malfunctioning of the processes associated with phenol metabolism in the soybean plants. We concluded that tolerance of legume-rhizobium symbiosis to water deprivation depends on mutual involvements of the both symbiotic partners – host plant and rhizobia, their ability to fully realize the defensive systems for activation of the key enzymatic complexes taking part in regulation of phenol metabolism in plants.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"25 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Pozdnyakova, A. Sailau, D. Solyanov, L. Aitisheva, Y. Tatina, V. Britko
Early flowering plants (ephemers and ephemeroids) are an important component of the biosystem of steppes and deserts. These species form perennial pasture communities, for early grazing. The present work was conducted to evaluate the floristic checklist and ecological uniqueness of early flowering plants of Central Kazakhstan, in the forest-steppe regions of Em-Bulak region and Edige mountains, during 2021 and 2022. A total of 26 species of ephemers and ephemeroids were recorded, which belong to 16 families: Liliaceae – 3 species, Asteraceae – 3 species, Ranunculaceae – 3 species, Rosaceae – 3 species, Brassicaceae – 2 species, Boraginaceae – 2 species, the other families are represented by one species. According to ecomorphological characteristics: 8 species (31%) belong to the xerophyte group, 6 (23%) to the xeromesophyte group, 7 (27%) to the mesoxerophyte group and 5 (19%) to the mesophyte group. Also, as a result of our research we found two species of plants which are included in the Red Book of the Republic of Kazakhstan – Pulsatilla patens (L.) Mill. and Tulipa patens Agardh. ex Schult. f. The data obtained can be used for environmental monitoring and issues of rational nature management of wild plants of Central Kazakhstan.
{"title":"Diversity of early flowering plants of the Ulytau mountains (Central Kazakhstan)","authors":"Y. Pozdnyakova, A. Sailau, D. Solyanov, L. Aitisheva, Y. Tatina, V. Britko","doi":"10.15421/012329","DOIUrl":"https://doi.org/10.15421/012329","url":null,"abstract":"Early flowering plants (ephemers and ephemeroids) are an important component of the biosystem of steppes and deserts. These species form perennial pasture communities, for early grazing. The present work was conducted to evaluate the floristic checklist and ecological uniqueness of early flowering plants of Central Kazakhstan, in the forest-steppe regions of Em-Bulak region and Edige mountains, during 2021 and 2022. A total of 26 species of ephemers and ephemeroids were recorded, which belong to 16 families: Liliaceae – 3 species, Asteraceae – 3 species, Ranunculaceae – 3 species, Rosaceae – 3 species, Brassicaceae – 2 species, Boraginaceae – 2 species, the other families are represented by one species. According to ecomorphological characteristics: 8 species (31%) belong to the xerophyte group, 6 (23%) to the xeromesophyte group, 7 (27%) to the mesoxerophyte group and 5 (19%) to the mesophyte group. Also, as a result of our research we found two species of plants which are included in the Red Book of the Republic of Kazakhstan – Pulsatilla patens (L.) Mill. and Tulipa patens Agardh. ex Schult. f. The data obtained can be used for environmental monitoring and issues of rational nature management of wild plants of Central Kazakhstan.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"62 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Schwartau, L. Mykhalska, T. Makoveychuk, V. Tretiakov
Ukraine is one of the world’s guarantors of food security and has the potential to further increase agricultural production. However, the vast majority of herbicides used on crops are acetolactate synthase (ALS) inhibitors, which poses a threat of herbicide-resistant weed species. The emergence and spread of herbicide-resistant weed biotypes can significantly increase the cost of growing crops to the point of loss of profitability. Herbicide resistance in barnyardgrass (Echinochloa crus-galli var. crus-galli) has been studied in long-term field and greenhouse experiments. Resistance of E. crus-galli to the ALS herbicide triazolopyrimidine derivative ‒ penoxsulam was identified. Expressed resistance was observed in weed plants grown from seeds collected under production conditions in Kherson region in 2015–2016 and 2020–2021. Cross resistance was observed for imidazolinone and sulfonylurea derivatives. It should be noted that the level of cross resistance to ALS herbicides was slightly higher for plants derived from weed seeds harvested in 2020–2021 compared to those harvested in 2015–2016. The introduction of a herbicidal composition of a herbicide mixture ‒ an inhibitor of 4-hydroxyphenylpyruvate dioxygenase (HPPD) (an enzyme in plants in the chain of carotenoid synthesis) ‒ mesotrione with an ALS-inhibitor (nicosulfuron) allowed effective control of the weed, which indicates the absence of multiple resistance to herbicides ‒ inhibitors of carotenoid synthesis. The high efficiency of E. crus-galli control was established by the application of herbicides ‒ inhibitors of fatty acid synthesis (graminicides). The highest level of efficiency in the experiments was observed with the application of fluazifop-butyl and somewhat less ‒ with pinoxaden. A tendency to reduce phytotoxicity to barnyardgrass from the south of Ukraine was observed with the introduction of tepraloxydim and quizalofop-ethyl. A lower level of phytotoxicity of fenoxaprop-p-ethyl on E. crus-galli should be noted compared to the effect of pinoxaden. No multiple resistance was observed with glyphosate (5-enolpyruvylshikimate-3-phosphate synthase inhibitor) and reglone (photosystem I inhibitor), allowing control of vegetative weeds at the beginning and end of the growing season. It has been established that monocot weed species have significantly increased their presence and harmfulness in agrophytocenoses in Ukraine and in the world since the 1950s with the widespread introduction of selective dicotyledonous species control with aryloxyphenoxyacetic, propionic and benzoic acid derivatives. This trend has been maintained until recently ‒ barnyardgrass is one of the dominant weed species in modern agrophytocenoses of Ukraine. Therefore, the identification of the ALS-resistant biotype of barnyardgrass complicates weed control in the following crops in rotations in the southern regions of the country: in maize crops with cross resistance to nicosulfuron, in sunflower ‒ to imidazolinones (
乌克兰是世界粮食安全的保障国之一，具有进一步提高农业产量的潜力。然而，绝大多数用于农作物的除草剂都是乙酰乳酸合成酶（ALS）抑制剂，这对抗性杂草物种构成了威胁。抗除草剂杂草生物型的出现和传播会大大增加农作物的种植成本，甚至丧失盈利能力。在长期的田间和温室实验中，对稗草（Echinochloa crus-galli var.crus-galli）的除草剂抗性进行了研究。研究发现，稗草对 ALS 除草剂三唑嘧啶衍生物五氟磺草胺具有抗药性。2015-2016 年和 2020-2021 年，在赫尔松地区生产条件下采集的种子培育出的杂草植株中观察到了表达的抗性。咪唑啉酮和磺酰脲类衍生物出现了交叉抗性。值得注意的是，与 2015-2016 年收获的杂草种子相比，2020-2021 年收获的杂草种子培育出的植物对 ALS 除草剂的交叉抗性水平略高。将除草剂混合物--4-羟基苯基丙酮酸二氧酶（HPPD）抑制剂（植物体内类胡萝卜素合成链中的一种酶）--间苯三酚与ALS抑制剂（烟嘧磺隆）--的除草组合物引入，可有效控制杂草，这表明对除草剂--类胡萝卜素合成抑制剂不存在多重抗性。施用除草剂-脂肪酸合成抑制剂（杀禾草剂）可高效控制 E. crus-galli。在实验中，使用氟唑草酮（fluazifop-butyl）的效率最高，而使用 pinoxaden 的效率稍低。使用 tepraloxydim 和 quizalofop-ethyl 后，乌克兰南部稗草的植物毒性呈下降趋势。需要注意的是，唑啉草酯（fenoxaprop-p-ethyl）对稗草（E. crus-galli）的植物毒性水平低于五唑醇（pinoxaden）。草甘膦（5-烯醇丙酮莽草酸-3-磷酸合成酶抑制剂）和雷公藤（光系统 I 抑制剂）没有产生多重抗性，因此可以在生长季节的初期和末期控制无性杂草。自 20 世纪 50 年代以来，随着使用芳氧基苯氧乙酸、丙酸和苯甲酸衍生物对双子叶杂草进行选择性控制的广泛引入，单子叶杂草物种在乌克兰和世界农田中的存在和危害性显著增加。这一趋势一直保持到最近--稗草是乌克兰现代农作物中的主要杂草物种之一。因此，抗 ALS 的稗草生物型的发现使该国南部地区轮作的以下作物的杂草控制工作变得复杂：玉米作物对烟嘧磺隆产生交叉抗性，向日葵对咪唑啉酮类（异丙威、异丙威）产生交叉抗性，水稻生产中也无法使用五氟磺草胺。传统上，使用合成助剂、苯氧乙酸衍生物等来控制 ALS 抗性。然而，在乌克兰赫尔松地区，施用 rinskor（氟吡禾草灵-苄基）的第三年，稻田里就发现了一些受抗 ALS 的稗草影响的杂草植株，而这些杂草在使用合成助剂后又恢复了生长。因此，在乌克兰赫尔松地区使用氟吡禾草灵苄基制剂来控制稗草对 ALS（五氟磺草胺等）的抗性已经受到限制。防止出现抗性杂草生物型的一个明显且经济可行的预防措施是实施 GAP（联合国粮农组织良好农业规范）方法：特别是使用不含杂草杂质的优质种子，增加农业技术除草措施的比例，恢复和扩大轮作，强制轮换使用不同作用模式的除草剂，在轮作中引入双子叶/豆科作物，在作物中单独或混合使用不同作用模式的除草剂。同时，农业技术措施和保护农田生物多样性应成为控制杂草抗药性的主要因素。使用具有不同作用模式的除草剂及其混合物是次要的。在乌克兰南部发现了对 ALS 具有高度抗性的 E. crus-galli，这表明仅使用单一作用机制的除草剂控制杂草的效果不佳，因此需要对该国的轮作原则和杂草控制方法进行重大修订，以保持农作物的高收益和高生产力水平。为了保持乌克兰作为全球粮食安全保障国之一的潜力，迫切需要解决这一问题。
{"title":"Identification of a herbicide-resistant biotype of Echinochloa crus-galli in Ukraine","authors":"V. Schwartau, L. Mykhalska, T. Makoveychuk, V. Tretiakov","doi":"10.15421/012334","DOIUrl":"https://doi.org/10.15421/012334","url":null,"abstract":"Ukraine is one of the world’s guarantors of food security and has the potential to further increase agricultural production. However, the vast majority of herbicides used on crops are acetolactate synthase (ALS) inhibitors, which poses a threat of herbicide-resistant weed species. The emergence and spread of herbicide-resistant weed biotypes can significantly increase the cost of growing crops to the point of loss of profitability. Herbicide resistance in barnyardgrass (Echinochloa crus-galli var. crus-galli) has been studied in long-term field and greenhouse experiments. Resistance of E. crus-galli to the ALS herbicide triazolopyrimidine derivative ‒ penoxsulam was identified. Expressed resistance was observed in weed plants grown from seeds collected under production conditions in Kherson region in 2015–2016 and 2020–2021. Cross resistance was observed for imidazolinone and sulfonylurea derivatives. It should be noted that the level of cross resistance to ALS herbicides was slightly higher for plants derived from weed seeds harvested in 2020–2021 compared to those harvested in 2015–2016. The introduction of a herbicidal composition of a herbicide mixture ‒ an inhibitor of 4-hydroxyphenylpyruvate dioxygenase (HPPD) (an enzyme in plants in the chain of carotenoid synthesis) ‒ mesotrione with an ALS-inhibitor (nicosulfuron) allowed effective control of the weed, which indicates the absence of multiple resistance to herbicides ‒ inhibitors of carotenoid synthesis. The high efficiency of E. crus-galli control was established by the application of herbicides ‒ inhibitors of fatty acid synthesis (graminicides). The highest level of efficiency in the experiments was observed with the application of fluazifop-butyl and somewhat less ‒ with pinoxaden. A tendency to reduce phytotoxicity to barnyardgrass from the south of Ukraine was observed with the introduction of tepraloxydim and quizalofop-ethyl. A lower level of phytotoxicity of fenoxaprop-p-ethyl on E. crus-galli should be noted compared to the effect of pinoxaden. No multiple resistance was observed with glyphosate (5-enolpyruvylshikimate-3-phosphate synthase inhibitor) and reglone (photosystem I inhibitor), allowing control of vegetative weeds at the beginning and end of the growing season. It has been established that monocot weed species have significantly increased their presence and harmfulness in agrophytocenoses in Ukraine and in the world since the 1950s with the widespread introduction of selective dicotyledonous species control with aryloxyphenoxyacetic, propionic and benzoic acid derivatives. This trend has been maintained until recently ‒ barnyardgrass is one of the dominant weed species in modern agrophytocenoses of Ukraine. Therefore, the identification of the ALS-resistant biotype of barnyardgrass complicates weed control in the following crops in rotations in the southern regions of the country: in maize crops with cross resistance to nicosulfuron, in sunflower ‒ to imidazolinones (","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"13 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139356422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Lykholat, N. О. Khromykh, O. V. Liashenko, T. V. Sklyar, A. O. Anishchenko, O. K. Balalaiev, T. A. Holubieva, T. Lykholat
The genus Sorbus L. is known for its extremely complex taxonomical relationships and health-promoting phytochemicals included in the composition of its floral constituents. The inflorescences of three Sorbus species (rowans), characterized by distinct molecular-genetic traits, were studied in order to examine the possible chemotaxonomic and antimicrobial value of their metabolites. GC–MS profiling of the hexane extracts of S. domestica, S. aucuparia, and S. torminalis inflorescences identified a total of 87 components, which represented six chemical classes (hydrocarbons, alcohols, esters, fatty acid, aldehydes, and ketones) and miscellaneous minor floral constituents (1-methylinosine, 5-amino tetrazole, 1,4-dimethylbenzene, 3,5-bis(1,1-dimethylethyl)-phenol, 3-acetoxy-7,8-epoxylanostan-11-ol, cycloeucalenol acetate, etc.). Principal component analysis (PCA) of the qualitative and quantitative heterogeneity of the floral metabolites determined 1-hentetracontanol, nonacosane, pentadecyl acrylate, 1-methylhexacosane, cycloeucalenol acetate, butyl acetate, and urs-12-ene as the main components which contributed to the differences between S. domestica, S. aucuparia and S. torminalis and resulted in the distinction between the rowan species. Disc-diffusion assays showed variability in activity of inflorescence extracts against Gram-negative (Enterobacter dissolvens, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae) and Gram-positive (Micrococcus lysodeikticus, Staphylococcus aureus, and S. epidermidis) bacterial and clinical fungal (Candida albicans) strains. The effect of S. torminalis was high against S. epidermidis and P. aeruginosa, while it was at its lowest against clinical C. albicans strains. Inflorescences of S. domestica showed the highest inhibition of P. aeruginosa, and moderate effects against S. epidermidis and C. albicans. Inflorescences of S. aucuparia caused low to moderate growth inhibition of both Gram-positive and Gram-negative bacterial strains, while it showed the highest effect on C. albicans. Antimicrobial properties of rowan inflorescences may be attributed to oleic, linoleic, arachidic, hexadecanoic, and pentadecanoic acids, 24-norursa-3,12-diene, hexahydrofarnesyl acetone, cycloeucalenol acetate, and other compounds which have known bioactivity. These findings indicated rowan inflorescences as a rich source of valuable secondary metabolites and allow us to assume an application of the floral constituents as chemotaxonomic markers of the genus Sorbus species.
山梨属（Sorbus L.）因其极其复杂的分类学关系及其花朵成分中包含的促进健康的植物化学物质而闻名。为了研究其代谢物可能具有的化学分类学价值和抗菌价值，我们研究了三种具有不同分子遗传特征的山梨属植物（小叶女贞）的花序。对 S. domestica、S. aucuparia 和 S. torminalis 花序的正己烷提取物进行了 GC-MS 分析。torminalis）花序的正己烷萃取物中鉴定出了 87 种成分，它们代表了六种化学类别（烃、醇、酯、脂肪酸、醛和酮）和其他次要成分、和酮类）以及其他次要花香成分（1-甲基肌苷、5-氨基四氮唑、1,4-二甲基苯、3,5-双（1,1-二甲基乙基）苯酚、3-乙酰氧基-7,8-环氧基木香烷-11-醇、环丁香烯醇乙酸酯等）。).对花代谢物的定性和定量异质性进行的主成分分析（PCA）确定，1-hentetracontanol、壬基二十烷、丙烯酸十五酯、1-甲基二十烷、乙酸环桉烯醇酯、乙酸丁酯和urs-12-ene 是造成 S.domestica、S. aucuparia 和 S. torminalis 之间差异的主要成分，也是区分楸树物种的主要成分。圆盘扩散试验表明，花序萃取物对革兰氏阴性（溶解肠杆菌、大肠杆菌、铜绿假单胞菌和肺炎克雷伯菌）和革兰氏阳性（溶血微球菌、金黄色葡萄球菌和表皮葡萄球菌）细菌和临床真菌（白色念珠菌）菌株的活性存在差异。托木纳尔菌对表皮葡萄球菌和绿脓杆菌的抗菌效果较高，而对临床白色念珠菌菌株的抗菌效果最低。S. domestica 的花序对绿脓杆菌的抑制率最高，对表皮葡萄球菌和白僵菌的抑制率中等。S. aucuparia 的花序对革兰氏阳性和革兰氏阴性细菌菌株的生长都有低到中等程度的抑制作用，而对白僵菌的抑制作用最大。花楸花序的抗菌特性可能归因于油酸、亚油酸、花生四烯酸、十六烷酸和十五烷酸、24-去甲-3,12-二烯、六氢法呢醇丙酮、环杜仲烯醇乙酸酯以及其他已知具有生物活性的化合物。这些发现表明花楸花序是宝贵的次生代谢物的丰富来源，并使我们能够将花朵成分作为山梨属物种的化学分类标记。
{"title":"Phytochemical profiles and antimicrobial activity of the inflorescences of Sorbus domestica, S. aucuparia, and S. torminalis","authors":"Y. Lykholat, N. О. Khromykh, O. V. Liashenko, T. V. Sklyar, A. O. Anishchenko, O. K. Balalaiev, T. A. Holubieva, T. Lykholat","doi":"10.15421/012333","DOIUrl":"https://doi.org/10.15421/012333","url":null,"abstract":"The genus Sorbus L. is known for its extremely complex taxonomical relationships and health-promoting phytochemicals included in the composition of its floral constituents. The inflorescences of three Sorbus species (rowans), characterized by distinct molecular-genetic traits, were studied in order to examine the possible chemotaxonomic and antimicrobial value of their metabolites. GC–MS profiling of the hexane extracts of S. domestica, S. aucuparia, and S. torminalis inflorescences identified a total of 87 components, which represented six chemical classes (hydrocarbons, alcohols, esters, fatty acid, aldehydes, and ketones) and miscellaneous minor floral constituents (1-methylinosine, 5-amino tetrazole, 1,4-dimethylbenzene, 3,5-bis(1,1-dimethylethyl)-phenol, 3-acetoxy-7,8-epoxylanostan-11-ol, cycloeucalenol acetate, etc.). Principal component analysis (PCA) of the qualitative and quantitative heterogeneity of the floral metabolites determined 1-hentetracontanol, nonacosane, pentadecyl acrylate, 1-methylhexacosane, cycloeucalenol acetate, butyl acetate, and urs-12-ene as the main components which contributed to the differences between S. domestica, S. aucuparia and S. torminalis and resulted in the distinction between the rowan species. Disc-diffusion assays showed variability in activity of inflorescence extracts against Gram-negative (Enterobacter dissolvens, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae) and Gram-positive (Micrococcus lysodeikticus, Staphylococcus aureus, and S. epidermidis) bacterial and clinical fungal (Candida albicans) strains. The effect of S. torminalis was high against S. epidermidis and P. aeruginosa, while it was at its lowest against clinical C. albicans strains. Inflorescences of S. domestica showed the highest inhibition of P. aeruginosa, and moderate effects against S. epidermidis and C. albicans. Inflorescences of S. aucuparia caused low to moderate growth inhibition of both Gram-positive and Gram-negative bacterial strains, while it showed the highest effect on C. albicans. Antimicrobial properties of rowan inflorescences may be attributed to oleic, linoleic, arachidic, hexadecanoic, and pentadecanoic acids, 24-norursa-3,12-diene, hexahydrofarnesyl acetone, cycloeucalenol acetate, and other compounds which have known bioactivity. These findings indicated rowan inflorescences as a rich source of valuable secondary metabolites and allow us to assume an application of the floral constituents as chemotaxonomic markers of the genus Sorbus species.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"82 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139357568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The term ‘assortative mating’ denotes situations when the choice of a mating partner is nonrandom and the phenotypes across mate pairs are correlated, either positively or negatively. Assortative mating has been registered as a particular type of behaviour in many taxa of animals, including various vertebrate and invertebrate species. The aim of this study is to conduct a meta-analysis of published data concerning assortative mating in various taxa of Gastropoda. In total, we found 36 published peer-reviewed papers that consider the size-assortative mating in Gastropoda. 32 species belonging to different taxonomic groups of this class were studied, which provided 58 cases for further analysis. The range of estimates of the strength of assortment between individuals for species included into our meta-analysis (46 cases) is very wide: from –0.155 (Brephulopsis cylindrica) to +0.966 (Veronicella sloanii). Integrally, for the studied species of Gastropoda, the average weighted estimate of the strength of assortment between the sizes of copulating individuals was 0.381 ± 0.014. It revealed that virtually all the points representing individual studies form a funnel-shaped dispersion on a scatterplot that lies along the line representing the estimate of the generalized mean rgen = 0.343 and uniformly fill the funnel-shaped space between the lines of 95% confidence interval of the correlation coefficient for a given sample size adjusted for the overall mean. The distribution of the estimates of the correlation coefficient between copulating individuals among the various gastropod species has a shape close to the normal distribution (Kolmogorov-Smirnov's d = 0.061; P > 0.20). Moreover, most estimates are concentrated within a range from 0.2 to 0.6. We found that the probability of obtaining reliable estimates of the correlation coefficient between the sizes of copulating individuals is dependent upon the number of pairs used in the analysis (binary logistic regression: χ2 = 8.92; df = 1; P = 0.0028). It can be argued that the existence of the negative size-assortative mating in Gastropoda has not yet been proved. On the other hand, if only statistically significant cases of size-assortative mating are considered (37 cases out of 58, or 63.7%), the average weighted estimate of the strength of assortment between sizes of copulating mollusks is 0.439 ± 0.015 (95% confidence interval: 0.409–0.468). If we consider the mating system and the environment simultaneously, the positive size-assortativity is most pronounced among the gonochoristic snails living in the aquatic environment (0.448 ± 0.021) while among the aquatic hermaphroditic species it is the weakest (0.315 ± 0.028). Terrestrial hermaphrodites (land snails and slugs) take the middle position. The numerous examples of the assortative mating with respect to different morphological traits, either quantitative or qualitative, have been described in various species of aquatic and land snails.
{"title":"Assortative mating in Gastropoda: A meta-analysis","authors":"S. Kramarenko, A. Kramarenko","doi":"10.15421/012330","DOIUrl":"https://doi.org/10.15421/012330","url":null,"abstract":"The term ‘assortative mating’ denotes situations when the choice of a mating partner is nonrandom and the phenotypes across mate pairs are correlated, either positively or negatively. Assortative mating has been registered as a particular type of behaviour in many taxa of animals, including various vertebrate and invertebrate species. The aim of this study is to conduct a meta-analysis of published data concerning assortative mating in various taxa of Gastropoda. In total, we found 36 published peer-reviewed papers that consider the size-assortative mating in Gastropoda. 32 species belonging to different taxonomic groups of this class were studied, which provided 58 cases for further analysis. The range of estimates of the strength of assortment between individuals for species included into our meta-analysis (46 cases) is very wide: from –0.155 (Brephulopsis cylindrica) to +0.966 (Veronicella sloanii). Integrally, for the studied species of Gastropoda, the average weighted estimate of the strength of assortment between the sizes of copulating individuals was 0.381 ± 0.014. It revealed that virtually all the points representing individual studies form a funnel-shaped dispersion on a scatterplot that lies along the line representing the estimate of the generalized mean rgen = 0.343 and uniformly fill the funnel-shaped space between the lines of 95% confidence interval of the correlation coefficient for a given sample size adjusted for the overall mean. The distribution of the estimates of the correlation coefficient between copulating individuals among the various gastropod species has a shape close to the normal distribution (Kolmogorov-Smirnov's d = 0.061; P > 0.20). Moreover, most estimates are concentrated within a range from 0.2 to 0.6. We found that the probability of obtaining reliable estimates of the correlation coefficient between the sizes of copulating individuals is dependent upon the number of pairs used in the analysis (binary logistic regression: χ2 = 8.92; df = 1; P = 0.0028). It can be argued that the existence of the negative size-assortative mating in Gastropoda has not yet been proved. On the other hand, if only statistically significant cases of size-assortative mating are considered (37 cases out of 58, or 63.7%), the average weighted estimate of the strength of assortment between sizes of copulating mollusks is 0.439 ± 0.015 (95% confidence interval: 0.409–0.468). If we consider the mating system and the environment simultaneously, the positive size-assortativity is most pronounced among the gonochoristic snails living in the aquatic environment (0.448 ± 0.021) while among the aquatic hermaphroditic species it is the weakest (0.315 ± 0.028). Terrestrial hermaphrodites (land snails and slugs) take the middle position. The numerous examples of the assortative mating with respect to different morphological traits, either quantitative or qualitative, have been described in various species of aquatic and land snails.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"12 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139357565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Dzhagan, T. Krupodorova, A. Atamanchuk, Y. Lytvynenko
Three pyrophilous discomycetes species (Anthracobia maurilabra (Cooke) Boud., Pyronema domesticum (Sowerby) Sacc. and Tricharina praecox (P. Karst.) Dennis) have been cultivated and studied in vitro. Cultures were obtained from fresh apothecia сollected in the Holosiivskyi National Nature Park (Kyiv, Ukraine). The culture growth and morphological characteristics of the studied fungi on different media (beer wort agar, Czapek Dox agar, potato-dextrose agar) were analyzed. All investigated species can quite easily grow under laboratory conditions on different tested nutrient media. Potato-dextrose agar was the most suitable medium for the enhancement of radial growth and the best expresses all the phenotypes of the colony of studied fungi. Macro- and micromorphological descriptions of all fungal colonies and illustrations are provided. The micromorphological analysis showed that common to the mycelium of all studied species of fungi was the presence of numerous drops of oil in the hyphae, anastomoses like T. praecox, а net- like structure of A. maurilabra similar to nematode capture hook. Beside this, A. maurilabra and P. domesticum formed the sexual stage under experimental conditions. Forming fruiting bodies of A. maurilabra in culture has not been reported before. Moreover, P. domesticum was found to form abundant dark brown sclerotia on potato-dextrose agar and Czapek Dox agar. Possible pigment composition in the P. domesticum sclerotia is discussed based on the Raman spectroscopy study, performed on this genus for the first time. The established cultural characteristics can be useful for taxonomic identification of fungal species and for pure quality control of mycelial cultures during their introduction, preservation and future potential applications in biotechnological areas.
对三种发热盘菌（Anthracobia maurilabra (Cooke) Boud.、Pyronema domesticum (Sowerby) Sacc.和Tricharina praecox (P. Karst.) Dennis）进行了体外培养和研究。培养物是从 Holosiivskyi 国家自然公园（乌克兰，基辅）采集的新鲜叶枕中获得的。研究人员分析了所研究真菌在不同培养基（啤酒麦芽汁琼脂、Czapek Dox 琼脂、马铃薯葡萄糖琼脂）上的生长情况和形态特征。在实验室条件下，所有研究的菌种都很容易在不同的营养培养基上生长。马铃薯葡萄糖琼脂是最适合促进径向生长的培养基，也最能体现所研究真菌菌落的所有表型。本文提供了所有真菌菌落的宏观和微观形态描述以及插图。微观形态分析表明，所有研究菌种的菌丝体都有一个共同点，即菌丝中存在大量油滴、吻合（如T. praecox）、A. maurilabra的网状结构（类似线虫捕捉钩）。此外，A. maurilabra 和 P. domesticum 还在实验条件下形成了有性阶段。以前从未报道过 A. maurilabra 在培养过程中形成子实体。此外，还发现 P. domesticum 在马铃薯葡萄糖琼脂和 Czapek Dox 琼脂上形成了大量黑褐色的硬菌。根据首次对该属进行的拉曼光谱研究，讨论了 P. domesticum 硬菌中可能存在的色素成分。已确定的培养特性可用于真菌物种的分类鉴定，以及菌丝培养物在引进、保存和未来在生物技术领域的潜在应用过程中的纯质量控制。
{"title":"Growth and morphological characteristics of some pyrophilous discomycetes in culture","authors":"V. Dzhagan, T. Krupodorova, A. Atamanchuk, Y. Lytvynenko","doi":"10.15421/012332","DOIUrl":"https://doi.org/10.15421/012332","url":null,"abstract":"Three pyrophilous discomycetes species (Anthracobia maurilabra (Cooke) Boud., Pyronema domesticum (Sowerby) Sacc. and Tricharina praecox (P. Karst.) Dennis) have been cultivated and studied in vitro. Cultures were obtained from fresh apothecia сollected in the Holosiivskyi National Nature Park (Kyiv, Ukraine). The culture growth and morphological characteristics of the studied fungi on different media (beer wort agar, Czapek Dox agar, potato-dextrose agar) were analyzed. All investigated species can quite easily grow under laboratory conditions on different tested nutrient media. Potato-dextrose agar was the most suitable medium for the enhancement of radial growth and the best expresses all the phenotypes of the colony of studied fungi. Macro- and micromorphological descriptions of all fungal colonies and illustrations are provided. The micromorphological analysis showed that common to the mycelium of all studied species of fungi was the presence of numerous drops of oil in the hyphae, anastomoses like T. praecox, а net- like structure of A. maurilabra similar to nematode capture hook. Beside this, A. maurilabra and P. domesticum formed the sexual stage under experimental conditions. Forming fruiting bodies of A. maurilabra in culture has not been reported before. Moreover, P. domesticum was found to form abundant dark brown sclerotia on potato-dextrose agar and Czapek Dox agar. Possible pigment composition in the P. domesticum sclerotia is discussed based on the Raman spectroscopy study, performed on this genus for the first time. The established cultural characteristics can be useful for taxonomic identification of fungal species and for pure quality control of mycelial cultures during their introduction, preservation and future potential applications in biotechnological areas.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"8 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139358154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leaf miners are increasingly causing damage to plants, often causing their death. There are about 10 thousand species of leaf miners in the world. The purpose of this study is to establish the location, species diversity, as well as the degree of damage caused by leaf miners to the leaves of Ulmus L. genus trees in the Pavlodar Region. In the course of the study, a visual inspection of the affected areas was used, as well as the selection, drying of damaged foliage and, subsequently, the removal of larvae and adult insects from it, followed by their study. During the vegetation seasons in 2019–2022, in Pavlodar, Ekibastuz and Aksu cities, as well as in Zhelezinka and Koktobe villages, elm leaves were studied for the presence of leaf miners, a visual inspection of the damage locations was conducted, and samples were taken, which later became the material for growing and detailed study of insects at different development stages. The article considers phyllophagous insects mining elm leaves in the North-East of Kazakhstan (Pavlodar Region) in 2019–2022. In the course of the conducted studies on the territory of the Pavlodar Region, 8 species belonging to 5 genera were identified: Stigmella Schrank, 1802, Orchestes Illiger, 1798, Fenusa Leach, 1817, Bucculatrix Zeller, 1839 and Phyllonorycter Hubner, 1822. Leaf miners that belong to the Agromyza genus of the Agromyzidae family were also identified. All representatives are obligate leaf miners, developing at the larval stage in the mesophyll of elm leaves. Most of the leaf-mining insects are represented by Agromyza and Stigmella species. They are among the most dangerous for the biocoenosis of the Pavlodar Region. Moreover, Agromyza insects have high plasticity and may become a problem in other regions. The obtained data will help to contribute to further studies in this direction, and also show the development degree of an understudied problem: the influence of leaf miners on the nature of Kazakhstan.
{"title":"Elm (Ulmus) leaf miners in North-East Kazakhstan","authors":"M. Zhussip, K. Akhmetov, U. Burkitbaeva","doi":"10.15421/012344","DOIUrl":"https://doi.org/10.15421/012344","url":null,"abstract":"Leaf miners are increasingly causing damage to plants, often causing their death. There are about 10 thousand species of leaf miners in the world. The purpose of this study is to establish the location, species diversity, as well as the degree of damage caused by leaf miners to the leaves of Ulmus L. genus trees in the Pavlodar Region. In the course of the study, a visual inspection of the affected areas was used, as well as the selection, drying of damaged foliage and, subsequently, the removal of larvae and adult insects from it, followed by their study. During the vegetation seasons in 2019–2022, in Pavlodar, Ekibastuz and Aksu cities, as well as in Zhelezinka and Koktobe villages, elm leaves were studied for the presence of leaf miners, a visual inspection of the damage locations was conducted, and samples were taken, which later became the material for growing and detailed study of insects at different development stages. The article considers phyllophagous insects mining elm leaves in the North-East of Kazakhstan (Pavlodar Region) in 2019–2022. In the course of the conducted studies on the territory of the Pavlodar Region, 8 species belonging to 5 genera were identified: Stigmella Schrank, 1802, Orchestes Illiger, 1798, Fenusa Leach, 1817, Bucculatrix Zeller, 1839 and Phyllonorycter Hubner, 1822. Leaf miners that belong to the Agromyza genus of the Agromyzidae family were also identified. All representatives are obligate leaf miners, developing at the larval stage in the mesophyll of elm leaves. Most of the leaf-mining insects are represented by Agromyza and Stigmella species. They are among the most dangerous for the biocoenosis of the Pavlodar Region. Moreover, Agromyza insects have high plasticity and may become a problem in other regions. The obtained data will help to contribute to further studies in this direction, and also show the development degree of an understudied problem: the influence of leaf miners on the nature of Kazakhstan.","PeriodicalId":44107,"journal":{"name":"Biosystems Diversity","volume":"57 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139358905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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