Recently, pomegranate fruit and its leaves have found wide usage as a natural treatment for several ailments. In this study, we investigated the restorative potentials of pomegranate (Punica granatum) peel extract against hepatic damage brought on by calcium tetrachloride (CCl 4 ) in rats and its effects on the human mutS homolog 2 (hMSH2) gene expression. Four groups of twenty male Wistar rats (n =5) were created at random. Group I was the untreated control group. Group II was given a dose of 0.4 ml CCl 4 intraperitoneally (IP) for two consecutive days/week for 3 weeks. Group III was given a daily dose (500 mg/ kg b.w) of pomegranate peel extract (PPE) for 3 weeks. Group IV received an IP of CCl 4 for two consecutive days/week for 3weeks followed by a daily oral dose of (500 mg/ kg b.w) of PPE for 3 weeks. Bodyweight, relative liver weight, serum AST, ALT, bilirubin, tissue GSH, and MDA, the expression of the hMSH2 gene, liver histology, and immunohistochemistry were assessed. Our results showed that the administration of CCl 4 to rats did not affect their body weight and relative liver weight. However, CCl 4 administration resulted in a decrease in tissue GSH, an increase in serum AST, ALT, bilirubin, lipid peroxidation, modification of liver histology, and immunohistochemistry, and the downregulation of the expression of the hMSH2 gene. Intriguingly, PPE treatment following CCl 4 administration to rats attenuated these changes. Taken together, our study reveals the potential of PPE for its use in the treatment of liver damage. administered group showed growing oval cells. c) Immunohistochemistry against CK7 for PPE only administered group, showing no visible oval cells. d) Immunohistochemistry against CK7 for CCl 4 + PPE group showed improved liver architecture to near normal with visible radiating hepatic cells. e) Immunohistochemistry against CK19 of the liver in the normal control group showed no visible oval cells. f) Immunohistochemistry against CK19 of the liver sections of the animals in the CCl 4 -administered group showed growing oval cells. g) Immunohistochemistry against CK19 for PPE only administered group, showing no visible oval cells. h) Immunohistochemistry against CK19 for CCl 4 + PPE group showed improved liver architecture to near normal with visible radiating hepatic cells. 4 administration to rats resulted in a decrease in serum liver damage biomarkers, restoration of liver histology and immunohistochemistry, an increase in the relative liver weight, and the upregulation of the hMSH2 gene expression. Taken together, our study reveals the potential of PPE for its use in the treatment of liver damage.
{"title":"The Role of Pomegranate Peel Extract in Improving Hepatotoxicity, and hMSH2 Expression in CCI 4 -Treated Rats","authors":"Dalia Mostafa Mohammed Domiaty","doi":"10.51847/z3ybdytchk","DOIUrl":"https://doi.org/10.51847/z3ybdytchk","url":null,"abstract":"Recently, pomegranate fruit and its leaves have found wide usage as a natural treatment for several ailments. In this study, we investigated the restorative potentials of pomegranate (Punica granatum) peel extract against hepatic damage brought on by calcium tetrachloride (CCl 4 ) in rats and its effects on the human mutS homolog 2 (hMSH2) gene expression. Four groups of twenty male Wistar rats (n =5) were created at random. Group I was the untreated control group. Group II was given a dose of 0.4 ml CCl 4 intraperitoneally (IP) for two consecutive days/week for 3 weeks. Group III was given a daily dose (500 mg/ kg b.w) of pomegranate peel extract (PPE) for 3 weeks. Group IV received an IP of CCl 4 for two consecutive days/week for 3weeks followed by a daily oral dose of (500 mg/ kg b.w) of PPE for 3 weeks. Bodyweight, relative liver weight, serum AST, ALT, bilirubin, tissue GSH, and MDA, the expression of the hMSH2 gene, liver histology, and immunohistochemistry were assessed. Our results showed that the administration of CCl 4 to rats did not affect their body weight and relative liver weight. However, CCl 4 administration resulted in a decrease in tissue GSH, an increase in serum AST, ALT, bilirubin, lipid peroxidation, modification of liver histology, and immunohistochemistry, and the downregulation of the expression of the hMSH2 gene. Intriguingly, PPE treatment following CCl 4 administration to rats attenuated these changes. Taken together, our study reveals the potential of PPE for its use in the treatment of liver damage. administered group showed growing oval cells. c) Immunohistochemistry against CK7 for PPE only administered group, showing no visible oval cells. d) Immunohistochemistry against CK7 for CCl 4 + PPE group showed improved liver architecture to near normal with visible radiating hepatic cells. e) Immunohistochemistry against CK19 of the liver in the normal control group showed no visible oval cells. f) Immunohistochemistry against CK19 of the liver sections of the animals in the CCl 4 -administered group showed growing oval cells. g) Immunohistochemistry against CK19 for PPE only administered group, showing no visible oval cells. h) Immunohistochemistry against CK19 for CCl 4 + PPE group showed improved liver architecture to near normal with visible radiating hepatic cells. 4 administration to rats resulted in a decrease in serum liver damage biomarkers, restoration of liver histology and immunohistochemistry, an increase in the relative liver weight, and the upregulation of the hMSH2 gene expression. Taken together, our study reveals the potential of PPE for its use in the treatment of liver damage.","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70842081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Q. Alshammari, Mezna AlHouty, B. Malik, M. Salih, Saleh Abdulkareem A. Alrumyan, M. Alshammari, Rahaf I. Alshareef, Elgeili Yousif
{"title":"Comparison between Magnetic Resonance Imaging and Ultrasound in Diagnosing Uterine Fibroids in Hail Area, Saudi Arabia","authors":"Q. Alshammari, Mezna AlHouty, B. Malik, M. Salih, Saleh Abdulkareem A. Alrumyan, M. Alshammari, Rahaf I. Alshareef, Elgeili Yousif","doi":"10.51847/5buvqymmxj","DOIUrl":"https://doi.org/10.51847/5buvqymmxj","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70812858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. A. Baz, Saeed H. Halawani, Ibrahim Abdulaziz, Majid Ali, Nhal Ahmed Baz, Mohammed Jafal, Khaldoun Saleh
Regorafenib is widely known as an oral tyrosine kinase inhibitor and antineoplastic agent. It acts on various tyrosine kinase receptors, including oncogenic, stromal, and angiogenic receptors. This study was conducted to determine the safety profile of regorafenib in King Abdullah Medical City. All patients who had received regorafenib in King Abdullah Medical City between December 2021 and May 2020 were included in the study. The data collected included patient demographics, diagnosis, regorafenib starting and escalated doses, reported adverse events, and associated management. Forty-two patients were found to be on regorafenib. The average age of the patients was 56 years (ranging from 38 to 73 years) of which 12 were females and 30 were males. The majority of the patients received the drug for metastatic colon cancer. The most common adverse event reported in our study was hyperbilirubinemia followed by fatigue. This was in comparison to the adverse events reported in the published literature. spasm and bilateral hydronephrosis were found to be the new adverse drug reactions, which were not reported in other studies. Half of the patients were reported to have discontinued the medication due to adverse events. Regorafenib as evidenced by the published studies and findings of our study was found to be effective in the management of advanced cancers in our local population. However, it was found to be associated with a variety of adverse events comparable to the published studies.
{"title":"Regorafenib Adverse Drug Reactions among Patients in King Abdullah Medical City; A Chart Review Study","authors":"H. A. Baz, Saeed H. Halawani, Ibrahim Abdulaziz, Majid Ali, Nhal Ahmed Baz, Mohammed Jafal, Khaldoun Saleh","doi":"10.51847/iexpv4xrns","DOIUrl":"https://doi.org/10.51847/iexpv4xrns","url":null,"abstract":"Regorafenib is widely known as an oral tyrosine kinase inhibitor and antineoplastic agent. It acts on various tyrosine kinase receptors, including oncogenic, stromal, and angiogenic receptors. This study was conducted to determine the safety profile of regorafenib in King Abdullah Medical City. All patients who had received regorafenib in King Abdullah Medical City between December 2021 and May 2020 were included in the study. The data collected included patient demographics, diagnosis, regorafenib starting and escalated doses, reported adverse events, and associated management. Forty-two patients were found to be on regorafenib. The average age of the patients was 56 years (ranging from 38 to 73 years) of which 12 were females and 30 were males. The majority of the patients received the drug for metastatic colon cancer. The most common adverse event reported in our study was hyperbilirubinemia followed by fatigue. This was in comparison to the adverse events reported in the published literature. spasm and bilateral hydronephrosis were found to be the new adverse drug reactions, which were not reported in other studies. Half of the patients were reported to have discontinued the medication due to adverse events. Regorafenib as evidenced by the published studies and findings of our study was found to be effective in the management of advanced cancers in our local population. However, it was found to be associated with a variety of adverse events comparable to the published studies.","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"10 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70824285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Practice, Perception and Satisfaction Regarding Mammography among Saudi Females","authors":"Bandar Salem Mana Alhindi","doi":"10.51847/kbuc8dsitj","DOIUrl":"https://doi.org/10.51847/kbuc8dsitj","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70826457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Almadfaa, Mohammed Khalid Alattas, Abdulellah I Aleissa, A. Bormah, H. M. Alqurashi, A. A. M. Felemban, Sultan Assiri, M. Alshehri, B. Alharbi
{"title":"An Overview on the Evaluation and Management of Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis","authors":"A. Almadfaa, Mohammed Khalid Alattas, Abdulellah I Aleissa, A. Bormah, H. M. Alqurashi, A. A. M. Felemban, Sultan Assiri, M. Alshehri, B. Alharbi","doi":"10.51847/k833tmzjqp","DOIUrl":"https://doi.org/10.51847/k833tmzjqp","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70826259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Himashree Bora, Madhu Kamle, S. Chopra, Pradeep Kumar
{"title":"Evaluation of Phytochemical Components, Antioxidant, and Antibacterial Activities of Coptis teeta Walls","authors":"Himashree Bora, Madhu Kamle, S. Chopra, Pradeep Kumar","doi":"10.51847/1jgpbykz1k","DOIUrl":"https://doi.org/10.51847/1jgpbykz1k","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70809839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Alhazmi, Fahad Alabbas, Hussain Alyami, Eid Alharbi, Abdullah Omar F Alilwit, Mohammed Ghamri, Abdullah AlGhamdi, Shuruq Rahil Alruwaili, O. Alghamdi, Othman Mohammed Alshahrani
{"title":"Sexual Dysfunction Related to Multiple Sclerosis: Literature Review","authors":"S. Alhazmi, Fahad Alabbas, Hussain Alyami, Eid Alharbi, Abdullah Omar F Alilwit, Mohammed Ghamri, Abdullah AlGhamdi, Shuruq Rahil Alruwaili, O. Alghamdi, Othman Mohammed Alshahrani","doi":"10.51847/9pjaplkbgz","DOIUrl":"https://doi.org/10.51847/9pjaplkbgz","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70815450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asma Alanazi, Fahad Wajdi, M. A. Al Issa, Albandari Abdulrahman Fallatah, Abdulrazaq Oudah Shaker, Abeer Abdalaziz AlHatim, M. Alqubali, Reem Hamoud Alshammari, B. Alghasham, Hassan Yousef Almohammedali
{"title":"An Overview on Klinefelter’s: Clinical Features and Management in Pediatric Population","authors":"Asma Alanazi, Fahad Wajdi, M. A. Al Issa, Albandari Abdulrahman Fallatah, Abdulrazaq Oudah Shaker, Abeer Abdalaziz AlHatim, M. Alqubali, Reem Hamoud Alshammari, B. Alghasham, Hassan Yousef Almohammedali","doi":"10.51847/grhpjq9tyy","DOIUrl":"https://doi.org/10.51847/grhpjq9tyy","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70821767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Alsukhayri, Raghd Biek, Rooz Abu Khozana, Bayan Algarni, Majed Ramadan, H. Alzahrani, Alanood S. Algarni
{"title":"Contributing Clinical Presentation, Risk Factors, and Outcomes for Diabetic Ketoacidosis Patients: A Single-Center Retrospective Study","authors":"B. Alsukhayri, Raghd Biek, Rooz Abu Khozana, Bayan Algarni, Majed Ramadan, H. Alzahrani, Alanood S. Algarni","doi":"10.51847/rdytddtsyn","DOIUrl":"https://doi.org/10.51847/rdytddtsyn","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70832324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Kale, Prakash Gulbhele, Vaibhav Gawade, Rahul Jagtap, Sudhir Khodade
{"title":"Detoxification of Gunja Seeds with Ex Vivo Study","authors":"N. Kale, Prakash Gulbhele, Vaibhav Gawade, Rahul Jagtap, Sudhir Khodade","doi":"10.51847/srbmuao7m1","DOIUrl":"https://doi.org/10.51847/srbmuao7m1","url":null,"abstract":"","PeriodicalId":46106,"journal":{"name":"International Journal of Pharmaceutical Research and Allied Sciences","volume":"11 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70834204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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