Pub Date : 2024-06-15DOI: 10.29244/currbiomed.2.2.61-70
Dwi Veni Endarwati, Asep Iin Nur Indra, Acep Tantan Hardiana, Yogi Khoirul Abror, Betty Nurhayati, Fusvita Merdekawati
Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.
{"title":"Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis","authors":"Dwi Veni Endarwati, Asep Iin Nur Indra, Acep Tantan Hardiana, Yogi Khoirul Abror, Betty Nurhayati, Fusvita Merdekawati","doi":"10.29244/currbiomed.2.2.61-70","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.61-70","url":null,"abstract":"Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141337413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-15DOI: 10.29244/currbiomed.2.2.93-100
Monica Silva Jerica, R. Tiuria, Nilla Mayasari, A. Nugraha, Mawar Subangkit
Latar Belakang: Ayam kampung merupakan salah satu kebutuhan protein hewani yang sering dicari masyarakat. Pemeliharaan ayam kampung dengan sistem sangkar bebas menjadi predisposisi terinfeksi oleh parasit gastrointestinal. Tujuan: Penelitian ini bertujuan mengetahui perubahan sel epitel dan hipertrofi sel goblet saluran pencernaan pada ayam kampung yang terinfeksi cacing cestoda Railletina spp secara alami. Metode: Penelitian ini menggunakan tujuh preparat arsip histopatologi usus halus ayam kampung yang terinfeksi cacing cestoda. Sampel usus halus ayam kampung diambil dari dua pasar yang berbeda yaitu pasar Pluit, Jakarta Utara dan pasar Kebayoran lama, Jakarta Selatan. Hasil: Hasil pengamatan secara histopatologi pada preparat usus ditemukan adanya deskuamasi epitel vili dan proliferasi sel-sel kripta yang diakibatkan oleh infeksi cacing cestoda. Jumlah sel goblet yang mengalami hipertrofi paling banyak ditemukan pada sampel preparat yang diambil dari pasar Kebayoran Lama, Jakarta Selatan walaupun tidak berpengaruh secara signifikan (P > 0,05) terhadap infeksi cestoda. Simpulan: Berdasarkan hasil penelitian ini dapat disimpulkan bahwa deskuamasi vili dan peningkatan jumlah hipertrofi sel goblet terjadi akibat infeksi cacing cestoda pada mukosa usus halus ayam kampung.
背景：土鸡是社会最需要的动物蛋白之一。土鸡饲养容易感染肠道寄生虫。研究目的本研究旨在确定自然感染铁线虫属绦虫的土鸡上皮细胞的变化和消化道鹅口疮细胞的肥大情况。研究方法本研究使用了 7 份感染了绦虫的土鸡小肠的档案组织病理学制备样本。样本取自两个不同的市场，分别是雅加达北部的 Pluit 市场和雅加达南部的 Kebayoran lama 市场。研究结果肠道制片的组织病理学观察结果显示，绦虫感染导致绒毛上皮脱落和隐窝细胞增生。在南雅加达 Kebayoran Lama 市场的制备样本中，发现肥大的鹅口疮细胞数量最多，尽管这对绦虫感染没有显著影响（P > 0.05）。结论根据本研究的结果，可以得出结论：本地鸡的小肠粘膜感染绦虫后会出现绒毛脱落和肥大的鹅口疮细胞数量增加。
{"title":"Hipertrofi sel goblet pada usus halus ayam kampung di pasar tradisional Jakarta yang terinfeksi cacing cestoda","authors":"Monica Silva Jerica, R. Tiuria, Nilla Mayasari, A. Nugraha, Mawar Subangkit","doi":"10.29244/currbiomed.2.2.93-100","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.93-100","url":null,"abstract":"Latar Belakang: Ayam kampung merupakan salah satu kebutuhan protein hewani yang sering dicari masyarakat. Pemeliharaan ayam kampung dengan sistem sangkar bebas menjadi predisposisi terinfeksi oleh parasit gastrointestinal. Tujuan: Penelitian ini bertujuan mengetahui perubahan sel epitel dan hipertrofi sel goblet saluran pencernaan pada ayam kampung yang terinfeksi cacing cestoda Railletina spp secara alami. Metode: Penelitian ini menggunakan tujuh preparat arsip histopatologi usus halus ayam kampung yang terinfeksi cacing cestoda. Sampel usus halus ayam kampung diambil dari dua pasar yang berbeda yaitu pasar Pluit, Jakarta Utara dan pasar Kebayoran lama, Jakarta Selatan. Hasil: Hasil pengamatan secara histopatologi pada preparat usus ditemukan adanya deskuamasi epitel vili dan proliferasi sel-sel kripta yang diakibatkan oleh infeksi cacing cestoda. Jumlah sel goblet yang mengalami hipertrofi paling banyak ditemukan pada sampel preparat yang diambil dari pasar Kebayoran Lama, Jakarta Selatan walaupun tidak berpengaruh secara signifikan (P > 0,05) terhadap infeksi cestoda. Simpulan: Berdasarkan hasil penelitian ini dapat disimpulkan bahwa deskuamasi vili dan peningkatan jumlah hipertrofi sel goblet terjadi akibat infeksi cacing cestoda pada mukosa usus halus ayam kampung.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141336446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-15DOI: 10.29244/currbiomed.2.2.101-115
Fiki Muhammad Ridho, Andika Julyanto Syachputra, Panggih Fahrudin, Andang Nurhuda, N. Nurliana, Nadhia S. Latuamury
Background: Current therapy for oral cancer (OC) patients, including surgery, radiotherapy, and chemotherapy, still have many shortcomings. Therefore, the discovery of natural products to prevent and treat cancer is receiving increasing attention, one of which is curcumin. Curcumin (diferuloylmethane) is a polyphenolic compound found in turmeric (Curcuma longa) and has been widely used as a herbal medicine because of its effects on health, one of which is as an anticancer agent. Objective: This study aimed to systematically and comprehensively review and summarize the anticancer effects and mechanisms of action involved of curcumin on OC cells. Methods: A systematic review methodology was employed adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines to review and summarize previous studies published in databases, including PubMed, ScienceDirect, and Google Scholar. The final results included 14 articles, both in vitro and in vivo studies. Results: Based on several preclinical studies regarding the effects of curcumin on OC cells, we highlight that curcumin has a strong potential in inhibiting OC cells through exerted effects such as immunomodulatory and anti-inflammatory effects, through inhibition of cell proliferation, invasion and migration, and angiogenesis, as well as through the induction of apoptosis and autophagy. Conclusion: The systematic review presented in this paper concludes that curcumin possesses the potential to inhibit the development of OC cells through several mechanisms of action related to immunomodulatory effects, anti-inflammatory effects, cell proliferation, invasion and migration, angiogenesis, apoptosis, and autophagy.
背景：目前对口腔癌（OC）患者的治疗，包括手术、放疗和化疗，仍有许多不足之处。因此，预防和治疗癌症的天然产品的发现正受到越来越多的关注，姜黄素就是其中之一。姜黄素（diferuloylmethane）是姜黄（Curcuma longa）中的一种多酚化合物，因其对健康的影响而被广泛用作草药，其中之一就是作为抗癌剂。研究目的本研究旨在系统、全面地回顾和总结姜黄素对 OC 细胞的抗癌作用及其作用机制。研究方法根据《系统综述和荟萃分析首选报告项目》（Preferred Reporting Items for Systematic Reviews and Meta-Analyses，PRISMA，2020）指南，采用系统综述方法，对发表在PubMed、ScienceDirect和Google Scholar等数据库中的以往研究进行综述和总结。最终结果包括 14 篇文章，既有体外研究，也有体内研究。研究结果基于姜黄素对 OC 细胞影响的几项临床前研究，我们强调姜黄素通过发挥免疫调节和抗炎作用，通过抑制细胞增殖、侵袭和迁移、血管生成，以及通过诱导细胞凋亡和自噬，在抑制 OC 细胞方面具有很强的潜力。结论本文的系统综述得出结论，姜黄素具有通过与免疫调节作用、抗炎作用、细胞增殖、侵袭和迁移、血管生成、细胞凋亡和自噬有关的几种作用机制抑制 OC 细胞发展的潜力。
{"title":"In vitro and in vivo effects of curcumin on oral cancer: a systematic review","authors":"Fiki Muhammad Ridho, Andika Julyanto Syachputra, Panggih Fahrudin, Andang Nurhuda, N. Nurliana, Nadhia S. Latuamury","doi":"10.29244/currbiomed.2.2.101-115","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.101-115","url":null,"abstract":"Background: Current therapy for oral cancer (OC) patients, including surgery, radiotherapy, and chemotherapy, still have many shortcomings. Therefore, the discovery of natural products to prevent and treat cancer is receiving increasing attention, one of which is curcumin. Curcumin (diferuloylmethane) is a polyphenolic compound found in turmeric (Curcuma longa) and has been widely used as a herbal medicine because of its effects on health, one of which is as an anticancer agent. Objective: This study aimed to systematically and comprehensively review and summarize the anticancer effects and mechanisms of action involved of curcumin on OC cells. Methods: A systematic review methodology was employed adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines to review and summarize previous studies published in databases, including PubMed, ScienceDirect, and Google Scholar. The final results included 14 articles, both in vitro and in vivo studies. Results: Based on several preclinical studies regarding the effects of curcumin on OC cells, we highlight that curcumin has a strong potential in inhibiting OC cells through exerted effects such as immunomodulatory and anti-inflammatory effects, through inhibition of cell proliferation, invasion and migration, and angiogenesis, as well as through the induction of apoptosis and autophagy. Conclusion: The systematic review presented in this paper concludes that curcumin possesses the potential to inhibit the development of OC cells through several mechanisms of action related to immunomodulatory effects, anti-inflammatory effects, cell proliferation, invasion and migration, angiogenesis, apoptosis, and autophagy.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141337300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Avocado (Persea americana) fruit has a high oil content, so it is widely used in the pharmaceutical and cosmetic industries. Objective: This study aims to determine the toxicity of avocado oil in mice using the lethal dose (LD50) method so that it can be used as a reference for further testing. Methods: This study used a total of 20 DDY strain female mice, which were divided into 5 groups: one control group and four treatment groups that were fed with avocado oil with 5, 10, 15, and 20 g/kg BW doses orally. The mortalities of experimental mice were observed for 14 days after treatment. Other parameters observed in this study were physiological response, body weight, absolute organ weight, and relative organ weight. Results: There was a change in behavior, and the obtained LD50 value was 25.4 g/kg BW. Observation of physiological responses, body weight, absolute organ weights, and relative organ weights showed no significant differences. Conclusion: It was concluded that avocado oil is considered relatively harmless and safe to use.
{"title":"Acute toxicity test of avocado (Persea americana) oil in mice","authors":"Aisya Salsa Bhila, Andriyanto Andriyanto, Bayu Febram Prasetyo","doi":"10.29244/currbiomed.2.2.55-60","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.55-60","url":null,"abstract":"Background: Avocado (Persea americana) fruit has a high oil content, so it is widely used in the pharmaceutical and cosmetic industries. Objective: This study aims to determine the toxicity of avocado oil in mice using the lethal dose (LD50) method so that it can be used as a reference for further testing. Methods: This study used a total of 20 DDY strain female mice, which were divided into 5 groups: one control group and four treatment groups that were fed with avocado oil with 5, 10, 15, and 20 g/kg BW doses orally. The mortalities of experimental mice were observed for 14 days after treatment. Other parameters observed in this study were physiological response, body weight, absolute organ weight, and relative organ weight. Results: There was a change in behavior, and the obtained LD50 value was 25.4 g/kg BW. Observation of physiological responses, body weight, absolute organ weights, and relative organ weights showed no significant differences. Conclusion: It was concluded that avocado oil is considered relatively harmless and safe to use. ","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141336172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Neutral buffered formalin (NBF) 10% fixative solution is widely used in histopathological slides. The fixation process generates liquid waste of NBF 10% and solid waste of tissue remnants. Objective: The research aimed to assess the reuse of NBF 10% fixative solution on the quality of histopathological slides and calculate the amount of waste produced. Methods: Treatments included single-use of fixative solution (control), reuse for 1, 2, and 3 times. Ten sample slides were prepared for each treatment, consisting of intestinal tissue, uterine fibroids, prostate, uterus, ovarian cyst, portio vaginalis cervicis, thyroid, rectum, breast fibroadenoma, and gallbladder tissues. Tissues were fixed with NBF 10% and processed histologically with hematoxylin-eosin staining. Liquid waste of NBF 10% and solid waste of tissue remnants were quantified. Histopathological slide quality was measured under a microscope for nuclear and cytoplasmic clarity, staining intensity, and color uniformity. Results: Control slides exhibited good quality with clearly blue-stained nuclei, pink cytoplasm, no color accumulation, and uniform staining across fields of view. Reused NBF 10% slides experienced a decrease in quality compared to the control but were still usable for diagnosis. Slides reused 2 and 3 times showed poor quality, making diagnosis difficult. Fixation resulted in 299.0 liters of liquid waste of NBF 10% and 64.9 kilograms of solid tissue remnants. Conclusion: Reusing NBF 10% decreases histological slide quality, though reuse once still allows for diagnosis. Reusing 10% NBF for tissue fixation can reduce the liquid waste of fixative solution and solid tissue waste.
{"title":"The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced","authors":"Zon Hardi, Wiwin Wiryanti, Adang Durachim, Mamat Rahmat","doi":"10.29244/currbiomed.2.2.71-83","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.71-83","url":null,"abstract":"Background: Neutral buffered formalin (NBF) 10% fixative solution is widely used in histopathological slides. The fixation process generates liquid waste of NBF 10% and solid waste of tissue remnants. Objective: The research aimed to assess the reuse of NBF 10% fixative solution on the quality of histopathological slides and calculate the amount of waste produced. Methods: Treatments included single-use of fixative solution (control), reuse for 1, 2, and 3 times. Ten sample slides were prepared for each treatment, consisting of intestinal tissue, uterine fibroids, prostate, uterus, ovarian cyst, portio vaginalis cervicis, thyroid, rectum, breast fibroadenoma, and gallbladder tissues. Tissues were fixed with NBF 10% and processed histologically with hematoxylin-eosin staining. Liquid waste of NBF 10% and solid waste of tissue remnants were quantified. Histopathological slide quality was measured under a microscope for nuclear and cytoplasmic clarity, staining intensity, and color uniformity. Results: Control slides exhibited good quality with clearly blue-stained nuclei, pink cytoplasm, no color accumulation, and uniform staining across fields of view. Reused NBF 10% slides experienced a decrease in quality compared to the control but were still usable for diagnosis. Slides reused 2 and 3 times showed poor quality, making diagnosis difficult. Fixation resulted in 299.0 liters of liquid waste of NBF 10% and 64.9 kilograms of solid tissue remnants. Conclusion: Reusing NBF 10% decreases histological slide quality, though reuse once still allows for diagnosis. Reusing 10% NBF for tissue fixation can reduce the liquid waste of fixative solution and solid tissue waste.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"5 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141336019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-15DOI: 10.29244/currbiomed.2.2.84-92
Fitrianingsih Saputra, Asep Iin Nur Indra, Ai Djuminar, Fusvita Merdekawati, Betty Nurhayati
Background: The Polymerase Chain Reaction (PCR) method can identify Mycobacterium tuberculosis in a sputum sample of a patient with TB (TB). One crucial step to ensure accurate PCR results is the DNA extraction process. Objective: The research aims to compare the concentration and purity of DNA from the sputum of TB patients using ultrasound and spin column extraction techniques. Methods: The research uses descriptive study designs with post-only design strategies. The primary data was derived from 18 sputum specimens from TB patients. Concentration measurement and DNA purity testing using a nanodrop spectroscopic photometer. Results: DNA extraction by ultrasound method has an average concentration of 18.9 ± 8.5 ng/L, with a peak of 37.6 ng/ L. The spin column method produces an average of 55.5 ± 27.9 ng/μL; the peak is 105.0 ng/ μL. The purity value of the DNA extract is in the range of 1.8 ± 2.0 with the ultrasound method of 61% and the spin column of 78%. Conclusion: The sonication method has a lower average concentration and a higher percentage of purity than the spin column method, and there are differences in concentrations and purity values between the two methods.
背景：聚合酶链式反应（PCR）方法可以从肺结核（TB）患者的痰液样本中鉴定结核分枝杆菌。确保 PCR 结果准确的一个关键步骤是 DNA 提取过程。研究目的本研究旨在比较使用超声波和旋柱提取技术从肺结核患者痰液中提取 DNA 的浓度和纯度。研究方法本研究采用描述性研究设计和仅后设计策略。主要数据来自 18 份肺结核患者的痰液标本。使用纳洛德罗光谱光度计进行浓度测量和 DNA 纯度检测。结果显示超声法提取 DNA 的平均浓度为 18.9 ± 8.5 ng/L，峰值为 37.6 ng/L；旋光柱法提取 DNA 的平均浓度为 55.5 ± 27.9 ng/μL，峰值为 105.0 ng/μL。DNA 提取物的纯度值在 1.8 ± 2.0 之间，其中超声法为 61%，旋光柱法为 78%。结论与旋柱法相比，超声法的平均浓度较低，纯度百分比较高，两种方法的浓度和纯度值存在差异。
{"title":"Concentration and purity of DNA extraction with sonication and spin column methods from the sputum sample of tuberculosis patient","authors":"Fitrianingsih Saputra, Asep Iin Nur Indra, Ai Djuminar, Fusvita Merdekawati, Betty Nurhayati","doi":"10.29244/currbiomed.2.2.84-92","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.84-92","url":null,"abstract":"Background: The Polymerase Chain Reaction (PCR) method can identify Mycobacterium tuberculosis in a sputum sample of a patient with TB (TB). One crucial step to ensure accurate PCR results is the DNA extraction process. Objective: The research aims to compare the concentration and purity of DNA from the sputum of TB patients using ultrasound and spin column extraction techniques. Methods: The research uses descriptive study designs with post-only design strategies. The primary data was derived from 18 sputum specimens from TB patients. Concentration measurement and DNA purity testing using a nanodrop spectroscopic photometer. Results: DNA extraction by ultrasound method has an average concentration of 18.9 ± 8.5 ng/L, with a peak of 37.6 ng/ L. The spin column method produces an average of 55.5 ± 27.9 ng/μL; the peak is 105.0 ng/ μL. The purity value of the DNA extract is in the range of 1.8 ± 2.0 with the ultrasound method of 61% and the spin column of 78%. Conclusion: The sonication method has a lower average concentration and a higher percentage of purity than the spin column method, and there are differences in concentrations and purity values between the two methods.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"8 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141337702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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