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Single Center "Snapshot" Experience With Donor-Derived Cell-Free DNA After Lung Transplantation. 单中心 "快照 "经验:肺移植术后的捐献者无细胞 DNA
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-09-16 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920958704
Deborah Jo Levine, David J Ross, Edward Sako
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引用次数: 0
Non-Targeted Metabolomic Analysis Reveals Serum Phospholipid Alterations in Patients with Early Stages of Diabetic Foot Ulcer. 非靶向代谢组学分析揭示早期糖尿病足溃疡患者血清磷脂改变
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-09-09 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920954828
Ignacio I Álvarez-Rodríguez, Eduardo Castaño-Tostado, David G García-Gutiérrez, Rosalía Reynoso-Camacho, Juana E Elton-Puente, Alicia Barajas-Pozos, Iza F Pérez-Ramírez

Diabetic foot ulcer (DFU) is a common complication of type 2 diabetes mellitus (T2DM) characterized by ulcer formation, which can lead to the amputation of lower extremities. However, the metabolic alterations related to this complication are not completely elucidated. Therefore, we carried out a metabolomic analysis of serum samples obtained from T2DM adult patients diagnosed with diabetic foot ulcer in a cross-sectional, observational, and comparative study. Eighty-four volunteers were classified into the following groups: without T2DM (control group, n = 30) and with T2DM and different stages of diabetic foot ulcer according to Wagner-Meggitt classification system: DFU G0 (n = 11), DFU G1 (n = 14), DFU G2 (n = 16), and DFU G3 (n = 13). The non-target metabolomic profile followed by chemometric analysis revealed that lysophosphatidylethanolamine (16:1) could be proposed as key metabolite related to the onset of diabetic foot ulcer; however, this phospholipid was not affected by diabetic foot ulcer progression. Therefore, further studies are necessary to validate these phospholipids as biomarker candidates for the early diagnosis of diabetic foot ulcer in T2DM patients.

糖尿病足溃疡(DFU)是2型糖尿病(T2DM)的常见并发症,以溃疡形成为特征,可导致下肢截肢。然而,与这种并发症相关的代谢改变尚未完全阐明。因此,我们对诊断为糖尿病足溃疡的T2DM成年患者的血清样本进行了代谢组学分析,这是一项横断面、观察和比较研究。84名志愿者按照Wagner-Meggitt分类系统分为:DFU G0 (n = 11)、DFU G1 (n = 14)、DFU G2 (n = 16)、DFU G3 (n = 13),无T2DM组(对照组,n = 30)和T2DM合并不同阶段糖尿病足溃疡组。非靶代谢组学分析和化学计量学分析显示溶血磷脂酰乙醇胺(16:1)可能是与糖尿病足溃疡发病相关的关键代谢物;然而,这种磷脂不受糖尿病足溃疡进展的影响。因此,需要进一步的研究来验证这些磷脂作为T2DM患者糖尿病足溃疡早期诊断的候选生物标志物。
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引用次数: 3
Fluid Biomarkers for Synaptic Dysfunction and Loss. 突触功能障碍和丧失的液体生物标志物。
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-08-21 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920950319
Elena Camporesi, Johanna Nilsson, Ann Brinkmalm, Bruno Becker, Nicholas J Ashton, Kaj Blennow, Henrik Zetterberg
Synapses are the site for brain communication where information is transmitted between neurons and stored for memory formation. Synaptic degeneration is a global and early pathogenic event in neurodegenerative disorders with reduced levels of pre- and postsynaptic proteins being recognized as a core feature of Alzheimer’s disease (AD) pathophysiology. Together with AD, other neurodegenerative and neurodevelopmental disorders show altered synaptic homeostasis as an important pathogenic event, and due to that, they are commonly referred to as synaptopathies. The exact mechanisms of synapse dysfunction in the different diseases are not well understood and their study would help understanding the pathogenic role of synaptic degeneration, as well as differences and commonalities among them and highlight candidate synaptic biomarkers for specific disorders. The assessment of synaptic proteins in cerebrospinal fluid (CSF), which can reflect synaptic dysfunction in patients with cognitive disorders, is a keen area of interest. Substantial research efforts are now directed toward the investigation of CSF synaptic pathology to improve the diagnosis of neurodegenerative disorders at an early stage as well as to monitor clinical progression. In this review, we will first summarize the pathological events that lead to synapse loss and then discuss the available data on established (eg, neurogranin, SNAP-25, synaptotagmin-1, GAP-43, and α-syn) and emerging (eg, synaptic vesicle glycoprotein 2A and neuronal pentraxins) CSF biomarkers for synapse dysfunction, while highlighting possible utilities, disease specificity, and technical challenges for their detection.
突触是大脑交流的场所,信息在神经元之间传递,并储存以形成记忆。突触变性是神经退行性疾病的全局和早期致病事件,突触前和突触后蛋白水平降低被认为是阿尔茨海默病(AD)病理生理的核心特征。与阿尔茨海默病一样,其他神经退行性和神经发育障碍也表现出突触稳态改变,这是一个重要的致病事件,因此它们通常被称为突触病变。突触功能障碍在不同疾病中的确切机制尚不清楚,他们的研究将有助于了解突触变性的致病作用,以及它们之间的差异和共同点,并突出特定疾病的候选突触生物标志物。脑脊液(CSF)突触蛋白的评估可以反映认知障碍患者的突触功能障碍,是一个感兴趣的领域。目前,大量的研究工作都是针对脑脊液突触病理的研究,以提高早期神经退行性疾病的诊断,并监测临床进展。在这篇综述中,我们将首先总结导致突触丧失的病理事件,然后讨论现有的突触功能障碍脑脊液生物标志物(如神经颗粒蛋白、SNAP-25、synaptotagmin-1、GAP-43和α-syn)和新出现的突触功能障碍脑脊液生物标志物(如突触囊泡糖蛋白2A和神经元戊烷素)的可用数据,同时强调它们检测的可能用途、疾病特异性和技术挑战。
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引用次数: 64
Simple Methods for Evaluating 4 Types of Biomarkers: Surrogate Endpoint, Prognostic, Predictive, and Cancer Screening. 评估4种生物标志物的简单方法:替代终点、预后、预测和癌症筛查。
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-08-04 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920946715
Stuart G Baker, Barnett S Kramer

We review simple methods for evaluating 4 types of biomarkers. First, we discuss the evaluation of surrogate endpoint biomarkers (to shorten a randomized trial) using 2 statistical and 3 biological criteria. Second, we discuss the evaluation of prognostic biomarkers (to predict the risk of disease) by comparing data collection costs with the anticipated net benefit of risk prediction. Third, we discuss the evaluation of predictive markers (to search for a promising subgroup in a randomized trial) using a multivariate subpopulation treatment effect pattern plot involving a risk difference or responders-only benefit function. Fourth, we discuss the evaluation of cancer screening biomarkers (to predict cancer in asymptomatic persons) using methodology to substantially reduce the sample size with stored specimens.

我们回顾了评价4种生物标志物的简单方法。首先,我们讨论了使用2个统计学标准和3个生物学标准评估替代终点生物标志物(缩短随机试验)。其次,我们通过比较数据收集成本和风险预测的预期净收益来讨论预后生物标志物的评估(以预测疾病风险)。第三,我们讨论了预测标记的评估(在随机试验中寻找有希望的亚组),使用多变量亚群治疗效果模式图,包括风险差异或仅应答者的益处函数。第四,我们讨论了癌症筛查生物标志物的评估(用于预测无症状患者的癌症),使用方法大幅减少储存标本的样本量。
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引用次数: 3
Interleukin-31 and Chronic Pruritus of Unknown Origin. 白细胞介素-31与不明原因的慢性瘙痒。
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-08 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920940712
Kanin Salao, Kittisak Sawanyawisuth, Kengkart Winaikosol, Charoen Choonhakarn, Suteeraporn Chaowattanapanit

Chronic pruritus of unknown origin (CPUO) is a refractory condition. The expression of Interleukin-31 (IL-31), a major pruritogenic cytokine, in CPUO patients has not been investigated. This study aimed to investigate the potential association of IL-31 with CPUO. This was a cross-sectional, analytical study. Patients diagnosed with CPUO and healthy subjects were included at a ratio of 1:2. Serum IL-31 levels were measured in both groups and compared. There were 10 CPUO and 20 healthy subjects who participated in this study. The median IL-31 level in the CPUO group was significantly higher than in the healthy group (127.3 vs 34.4 pg/mL; P < .001). The presence of CPUO was independently associated with IL-31 levels with a coefficient of 89.678 (P < .001). The serum IL-31 cutoff point for CPUO was 56.8 pg/mL, with an area under the receiver operating characteristic curve (ROC) of 100%. Chronic pruritus of unknown origin was significantly and independently associated with higher IL-31 levels. Further clinical trials of IL-31-related treatment may be justified in CPUO patients.

来源不明的慢性瘙痒症(CPUO)是一种难治性疾病。白细胞介素-31 (IL-31),一种主要的致瘙痒细胞因子,在CPUO患者中的表达尚未被研究。本研究旨在探讨IL-31与CPUO的潜在关联。这是一项横断面分析研究。诊断为CPUO的患者与健康受试者按1:2的比例入组。测定两组患者血清IL-31水平并进行比较。共有10名CPUO受试者和20名健康受试者参加本研究。CPUO组IL-31的中位水平显著高于健康组(127.3 vs 34.4 pg/mL;P P
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引用次数: 11
High-Quality and High-Yield RNA Extraction Method From Whole Human Saliva. 人全唾液高质量、高产率RNA提取方法。
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-06-08 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920929705
Vaibhav Gandhi, Mara H O'Brien, Sumit Yadav

Background: Human saliva has been identified as a novel, practical, and noninvasive source of biomarkers and genetic materials. However, it is equally challenging due to the availability of an abundance of impurities in the form of microbes and other proteinaceous compounds. The objective of this study was to develop a robust, reproducible, and economic method of extracting high-yield and high-quality RNA from whole human saliva.

Methods: The modified TRIzol protocol was developed to extract RNA from saliva (n = 14), followed by complementary DNA synthesis and reverse transcription quantitative polymerase chain reaction analyses for the genes encoding IL1B, ALPL, RUNX2, and ACTB. To compare our protocol with the spin column-based method, we used Qiagen Salivary Protect Micro-RNA spin columns (n = 6). To evaluate and compare the yields and quality of extracted RNAs from both methods, we used (1) Experion Bioanalyzer, (2) QuantiFluor RNA dye, and (3) NanoDrop 2000 Spectrometer.

Results: With the modified TRIzol lysis protocol, a high yield of total RNA, on average 12.34 μg, from saliva was extracted compared with on average 0.2 μg with a spin column-based method. The average RQI (RNA quality index) with the TRIzol method was 7.86, which is also comparable with that of the spin column-based method (RQI = 7.58). QuantiFluor dye used for RNA quantification showed a 16-fold higher yield of RNA concentration using our TRIzol protocol.

Conclusions: Our modified TRIzol protocol is a reproducible method to extract RNA from whole human saliva which can be used for gene expression analysis. This method allows also ensures the quality of RNA required for specific applications such as RNA sequencing.

背景:人类唾液已被确定为一种新颖、实用、无创的生物标志物和遗传物质来源。然而,由于微生物和其他蛋白质化合物形式的大量杂质的可用性,这同样具有挑战性。本研究的目的是建立一种可靠的、可重复的、经济的从人唾液中提取高产量和高质量RNA的方法。方法:采用改进的TRIzol方法提取唾液RNA (n = 14),对IL1B、ALPL、RUNX2和ACTB编码基因进行互补DNA合成和逆转录定量聚合酶链反应分析。为了与基于自旋柱的方法进行比较,我们使用Qiagen Salivary Protect Micro-RNA自旋柱(n = 6)。为了评估和比较两种方法提取的RNA的产量和质量,我们使用了(1)Experion生物分析仪,(2)QuantiFluor RNA染料和(3)NanoDrop 2000光谱仪。结果:采用改进的TRIzol裂解方案,从唾液中提取总RNA的平均产量为12.34 μg,而采用自旋柱法提取总RNA的平均产量为0.2 μg。TRIzol法的平均RQI (RNA质量指数)为7.86,与基于自旋柱的方法(RQI = 7.58)相当。用于RNA定量的quantifluer染料显示,使用我们的TRIzol协议,RNA浓度的产量提高了16倍。结论:改进的TRIzol方法是一种可重复提取人唾液RNA的方法,可用于基因表达分析。这种方法还可以确保特定应用(如RNA测序)所需的RNA质量。
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引用次数: 7
Glucose Homeostasis in Obese Women Is Not Associated to Unacylated Ghrelin Plasma Levels. 肥胖女性的葡萄糖稳态与未酰化胃饥饿素血浆水平无关。
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-06-05 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920928923
Luisa Veiga, Miguel Brito, Carina Silva, José Silva-Nunes

Introduction: Unacylated ghrelin (UAG) is the major form of circulating ghrelin. Initially considered as a nonfunctional peptide, soon after, UAG has been associated to an insulin sensitizing action and to a negative action on energy balance. The aim of this study was to analyze the association between the serum levels of UAG and glucose metabolism parameters in obese women, independently from eventual influence of anthropometrics.

Methods: One hundred lean and 254 obese Caucasian women were studied. Each woman was characterized for anthropometrics, fasting glucose, insulin, HbA1c, and UAG. In addition, obese women were subjected to a classic oral glucose tolerance test (oGTT) to assess glucose and insulin at 120 minutes. Insulin resistance was assessed by the homeostasis model assessment (HOMA-IR). Obese women were classified in 3 glycemic status subgroups (normoglycemia, prediabetes, and diabetes) according to HbA1c and to fasting and oGTT glucose values.

Results: In comparison with the lean group, significantly lower levels of UAG were observed in obese women. However, no significant difference was observed through obesity classes I to III. UAG levels were not significantly different among glycemic status subgroups and did not show any association with glucose, insulin, HOMA-IR, or HbA1c.

Conclusions: Although anthropometry can influence the level of the unacylated form of ghrelin, UAG plasma levels do not associate to glucose homeostasis parameters.

Unacylated ghrelin (UAG)是循环胃饥饿素的主要形式。最初被认为是一种无功能肽,不久之后,UAG与胰岛素增敏作用和能量平衡的负面作用有关。本研究的目的是分析肥胖妇女血清UAG水平与葡萄糖代谢参数之间的关系,独立于人体测量学的最终影响。方法:对100名白种女性进行研究,并对254名肥胖女性进行研究。每位女性的特征包括人体测量、空腹血糖、胰岛素、糖化血红蛋白和UAG。此外,肥胖妇女接受经典的口服葡萄糖耐量试验(oGTT),以评估120分钟的葡萄糖和胰岛素。胰岛素抵抗通过稳态模型评估(HOMA-IR)进行评估。根据HbA1c、空腹和oGTT血糖值,将肥胖女性分为3个血糖状态亚组(血糖正常、糖尿病前期和糖尿病)。结果:与瘦子组相比,肥胖妇女UAG水平明显降低。然而,在I级和III级肥胖中没有观察到显著差异。UAG水平在血糖状态亚组之间没有显著差异,与葡萄糖、胰岛素、HOMA-IR或HbA1c没有任何关联。结论:尽管人体测量可以影响胃饥饿素未酰化形式的水平,但UAG血浆水平与葡萄糖稳态参数无关。
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引用次数: 3
Marked Elevation in Serum Procalcitonin Levels Do Not Correlate With Severity of Disease or Mortality in Hospitalized Patients: A Retrospective Study. 血清降钙素原水平显著升高与住院患者疾病严重程度或死亡率无关:一项回顾性研究
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-05-15 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920917941
Richard J Durrance, Tofura Ullah, Harsh Patel, Grace Martinez, Kelly Cervellione, Veronica B Zafonte, Khalid Gafoor, Farshad Bagheri

Background: Bacteremia and sepsis are significant contributors to the morbidity, mortality, and economic burden of health care systems worldwide. Procalcitonin has been identified as a potentially useful marker of disease and severity in sepsis. However, the assumption that greater procalcitonin levels correlate with greater burden of disease has not been adequately studied.

Methods: A retrospective chart review of adult patients admitted to an urban teaching hospital with suspected sepsis was undertaken to test the association of elevated procalcitonin (>30 ng/mL) with other markers of sepsis (lactic acid, white blood cell count, percent bands), severity of disease (Sequential Organ Failure Assessment [SOFA] and Acute Physiology and Chronic Health Evaluation-II [APACHE II] scores), and mortality.

Results: In total, 168 patients were identified over 18 months (42% ward, 11% Stepdown, 44% medical intensive care unit [MICU], 2% surgical intensive care unit (STICU), 1% gynecology [GYN]). The Spearman correlation analysis showed that serum procalcitonin level did not correlate with SOFA (P = .238) or APACHE II (P = .918) scores on admission, and did not correlate with survival (Kruskal-Wallis test, P = .937). However, higher serum procalcitonin levels were associated with patients who had positive blood cultures (Kruskal-Wallis test, P = .0016 for Gram-positive and P = .0007 for Gram-negative bacteria). Lactic acid levels on admission strongly correlated with SOFA APACHE II (the Spearman correlation, P < .0001 for both) and mortality (P = .0001 for both).

Conclusions: Higher serum procalcitonin levels above 30 ng/mL failed to correlate with indicators of sepsis, severity of disease (SOFA and APACHE II scores), and mortality but were associated with positive blood cultures. Lactic acid levels did show correlation to both severity of disease and mortality. Serum procalcitonin levels >30 ng/mL do not appear to correlate with the severity of disease in a sample of patients with markedly elevated initial procalcitonin levels.

背景:菌血症和败血症是全球卫生保健系统发病率、死亡率和经济负担的重要贡献者。降钙素原已被确定为潜在有用的疾病和严重程度的标记在败血症。然而,更高的降钙素原水平与更大的疾病负担相关的假设尚未得到充分的研究。方法:对一所城市教学医院疑似脓毒症的成年患者进行回顾性图表回顾,以检测降钙素原升高(>30 ng/mL)与脓毒症的其他标志物(乳酸、白细胞计数、百分比带)、疾病严重程度(顺序器官衰竭评估[SOFA]和急性生理和慢性健康评估-II [APACHE II]评分)和死亡率的关系。结果:在18个月内共发现168例患者(42%为病房,11%为转诊病房,44%为内科重症监护病房,2%为外科重症监护病房,1%为妇科)。Spearman相关分析显示,血清降钙素原水平与入院时SOFA (P = .238)或APACHE II (P = .918)评分无关,与生存无关(Kruskal-Wallis检验,P = .937)。然而,较高的血清降钙素原水平与血培养阳性的患者相关(Kruskal-Wallis试验,P =。革兰氏阳性0.0016,P =。0007代表革兰氏阴性菌)。入院时乳酸水平与SOFA APACHE II密切相关(Spearman相关,P P =。两者都是0001)。结论:血清降钙素原水平高于30 ng/mL与脓毒症、疾病严重程度(SOFA和APACHE II评分)和死亡率指标没有相关性,但与血培养阳性相关。乳酸水平确实与疾病的严重程度和死亡率相关。血清降钙素原水平>30 ng/mL似乎与初始降钙素原水平显著升高的患者样本中疾病的严重程度无关。
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引用次数: 4
Allostatic Load Indices With Cholesterol and Triglycerides Predict Disease and Mortality Risk in Zoo-Housed Western Lowland Gorillas (Gorilla gorilla gorilla). 胆固醇和甘油三酯的代谢负荷指数可预测动物园饲养的西部低地大猩猩(大猩猩)的疾病和死亡风险。
IF 3.8 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-05-03 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920914585
Ashley N Edes, Katie L Edwards, Barbara A Wolfe, Janine L Brown, Douglas E Crews

Allostatic load, or the physiological dysregulation accumulated due to senescence and stress, is an established predictor of human morbidity and mortality and has been proposed as a tool for monitoring health and welfare in captive wildlife. It is estimated by combining biomarkers from multiple somatic systems into allostatic load indices (ALIs), providing a score representing overall physiological dysregulation. Such ALIs have been shown to predict disease and mortality risk in western lowland gorillas. In these prior analyses, we were unable to include lipid markers, a potential limitation as they are key biomarkers in human models. Recently, we were able to assay serum cholesterol and triglycerides and add them to our previous ALI. We then re-examined associations with health outcomes using binomial generalized linear models. We constructed ALIs using 2 pooling strategies and 2 methods. By itself, a 1-unit increase in allostatic load was associated with higher odds of all-cause morbidity and mortality, but results were mixed for cardiac disease. However, the best fit models for all-cause morbidity and cardiac disease included only age and sex. Allostatic load was retained alongside age in the best fit models for mortality, with a 1-unit increase associated with 23% to 45% higher odds of death. Compared with previous results, ALIs containing cholesterol and triglycerides better predict disease risk in zoo-housed western lowland gorillas, as evidenced by larger effect sizes for some models and better goodness of fit for all ALIs. Based on these results, we address methodology for future allostatic load research on wildlife.

静态负荷,或因衰老和压力而累积的生理失调,是人类发病率和死亡率的既定预测指标,也被提议作为监测圈养野生动物健康和福利的工具。它是通过将多个躯体系统的生物标志物组合成异位负荷指数(ALIs)来估算的,提供一个代表整体生理失调的分数。这种 ALIs 已被证明可以预测西部低地大猩猩的疾病和死亡风险。在以前的分析中,我们无法将脂质标记物包括在内,这是一个潜在的局限性,因为脂质标记物是人类模型中的关键生物标记物。最近,我们能够检测血清胆固醇和甘油三酯,并将它们添加到之前的 ALI 中。然后,我们使用二叉广义线性模型重新研究了与健康结果的关联。我们使用 2 种汇总策略和 2 种方法构建了 ALI。就其本身而言,代谢负荷每增加 1 个单位,全因发病率和死亡率就会升高,但心脏疾病的结果则不尽相同。然而,全因发病率和心脏病的最佳拟合模型仅包括年龄和性别。在死亡率的最佳拟合模型中,静态负荷与年龄一起被保留,每增加 1 个单位,死亡几率就会增加 23% 至 45%。与之前的结果相比,含有胆固醇和甘油三酯的 ALIs 能更好地预测动物园饲养的西部低地大猩猩的疾病风险,这表现在某些模型的效应大小更大,所有 ALIs 的拟合度更好。基于这些结果,我们探讨了未来对野生动物进行静态负荷研究的方法。
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引用次数: 0
Detecting Prostate Cancer Using Pattern Recognition Neural Networks With Flow Cytometry-Based Immunophenotyping in At-Risk Men. 使用模式识别神经网络和基于流式细胞术的免疫表型在高危男性中检测前列腺癌。
IF 3.4 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-04-17 eCollection Date: 2020-01-01 DOI: 10.1177/1177271920913320
George A Dominguez, Alexander T Polo, John Roop, Anthony J Campisi, Robert A Somer, Adam D Perzin, Dmitry I Gabrilovich, Amit Kumar

Current screening methods for prostate cancer (PCa) result in a large number of false positives making it difficult for clinicians to assess disease status, thus warranting advancements in screening and early detection methods. The goal of this study was to design a liquid biopsy test that uses flow cytometry-based immunophenotyping and artificial neural network (ANN) analysis to detect PCa. Numerous myeloid and lymphoid cell populations, including myeloid-derived suppressor cells, were measured from 156 patients with PCa, 123 with benign prostatic hyperplasia (BPH), and 99 male healthy donor (HD) controls. Using pattern recognition neural network (PRNN) analysis, a type of ANN, PCa detection compared against HD resulted in 96.6% sensitivity, 87.5% specificity, and an area under the curve (AUC) value of 0.97. Detecting patients with higher risk disease (⩾Gleason 7) against lower risk disease (BPH/Gleason 6) resulted in 92.0% sensitivity, 42.7% specificity, and an AUC of 0.72. This study suggests that analyzing flow cytometry immunophenotyping data with PRNNs may prove to be a useful tool to improve PCa detection and reduce the number of unnecessary prostate biopsies performed each year.

目前的前列腺癌(PCa)筛查方法导致大量假阳性,使临床医生难以评估疾病状态,因此需要在筛查和早期检测方法方面取得进展。本研究的目的是设计一种液体活检测试,使用基于流式细胞术的免疫表型和人工神经网络(ANN)分析来检测PCa。对156例PCa患者、123例良性前列腺增生(BPH)患者和99例男性健康供体(HD)对照进行了大量髓系和淋巴细胞群(包括髓系来源的抑制细胞)检测。采用模式识别神经网络(PRNN)分析,与HD相比,PCa检测的灵敏度为96.6%,特异性为87.5%,曲线下面积(AUC)值为0.97。检测高风险疾病(小于或等于Gleason 7)的患者与低风险疾病(BPH/Gleason 6)的对比,导致92.0%的敏感性,42.7%的特异性,AUC为0.72。这项研究表明,用prnn分析流式细胞术免疫表型数据可能是一种有用的工具,可以提高前列腺癌的检测水平,减少每年不必要的前列腺活检次数。
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引用次数: 0
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