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Analysis of Physicochemical Properties of Corn Starch Based Composite Biodegradable Cups Influenced by Ultrasonication Pretreatment of Casting Solutions 基于玉米淀粉的复合生物可降解杯的理化特性分析--浇铸溶液的超声波预处理对其的影响
Pub Date : 2024-08-10 DOI: 10.9734/acri/2024/v24i6813
Ankit Deshmukh, G. Talwar
Biodegradable cup is made using a casting solution which is made up of mixture of corn starch (7%), whey protein concentrate(3%), carboxy methyl cellulose (CMC) (2%), and glycerol (4%). This research work focused on analysing the effect of ultrasonication treatment with varying amplitude levels (0%, 20%, 40%, and 60%) and time durations (0, 1, 2, 4, 8, and 16 minutes) on the casting solution for the preparation of biodegradable cups. The study examined the impact of ultrasonication on physical properties such as viscosity, thickness, density and chemical properties such as solubility in water, opacity of the cups. The viscosity of the casting solution decreased as ultrasonication treatment intensity increased, resulting in a decrease in the thickness of the cups. Higher ultrasonication intensity also led to an increase in solubility of cup samples in water. The density of cups, produced through the application of ultrasonication during the preparation process, exhibited an augmentation with elevated ultrasonication intensity and prolonged treatment duration. Ultrasonication treatment caused a decrease in opacity, resulting in a more transparent cup material. Overall, the findings indicate that ultrasonication may be used to streamline the preparation process while enhancing the physical characteristics of biodegradable cups.
生物降解杯是用浇注液制成的,浇注液由玉米淀粉(7%)、浓缩乳清蛋白(3%)、羧甲基纤维素(CMC)(2%)和甘油(4%)混合而成。这项研究工作的重点是分析不同振幅水平(0%、20%、40% 和 60%)和不同持续时间(0、1、2、4、8 和 16 分钟)的超声处理对用于制备生物降解杯的浇铸溶液的影响。研究考察了超声波对杯子的物理特性(如粘度、厚度、密度)和化学特性(如水溶性、不透明度)的影响。随着超声处理强度的增加,浇铸溶液的粘度降低,导致杯子的厚度减小。超声处理强度越高,杯子样品在水中的溶解度也越高。在制备过程中应用超声波处理产生的杯子密度随着超声波处理强度的增加和处理时间的延长而增加。超声处理降低了不透明度,使杯子材料更加透明。总之,研究结果表明,超声处理可用于简化制备过程,同时增强生物降解杯的物理特性。
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引用次数: 0
Multidimensional Scaling Method and Some Practical Applications 多维标度法和一些实际应用
Pub Date : 2024-08-10 DOI: 10.9734/acri/2024/v24i6814
B. Manjunatha., Appaji Pundalik Naik, K. R. Mahendra, M. S., G. H., N. R. Kiran, Damodhara G. N., Karthik R.
Multi-Dimensional Scaling (MDS) is a data visualization method that identifies clusters of points by representing the distances or dissimilarities between sets of objects in a lower-dimensional space. This paper explores the theoretical concepts of MDS, various methods of implementation, and the analytical processes involved. Emphasis is placed on the "Stress" function, a goodness-of-fit metric that quantifies the discrepancy between distances in high-dimensional and lower-dimensional spaces. Practical examples and detailed procedures for implementing MDS using MS-Excel and R are provided to enhance understanding. The paper also discusses the use of Scree-plots for determining the optimal number of dimensions. Applications of MDS in different fields, including marketing, ecology, molecular biology, and social networks, are presented with examples on Perceptions of Nations data and Morse code confusion data. Additionally, as a significant contribution, a case study on factors affecting agricultural productivity is included. The versatility and utility of MDS in simplifying complex data and facilitating better decision-making are demonstrated through these practical applications and software implementations.
多维缩放(MDS)是一种数据可视化方法,它通过表示低维空间中对象集之间的距离或差异来识别点群。本文探讨了 MDS 的理论概念、各种实现方法以及相关的分析过程。重点放在 "Stress "函数上,这是一个拟合度量,用于量化高维空间和低维空间中的距离差异。文中提供了使用 MS-Excel 和 R 实现 MDS 的实用示例和详细步骤,以加深理解。本文还讨论了如何使用屏幕图确定最佳维数。文章介绍了 MDS 在不同领域的应用,包括市场营销、生态学、分子生物学和社交网络,并以 "对国家的看法 "数据和莫尔斯电码混淆数据为例进行了说明。此外,作为一项重要贡献,还包括一项关于影响农业生产力因素的案例研究。通过这些实际应用和软件实现,展示了 MDS 在简化复杂数据和促进更好决策方面的多功能性和实用性。
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引用次数: 0
Multidimensional Scaling Method and Some Practical Applications 多维标度法和一些实际应用
Pub Date : 2024-08-10 DOI: 10.9734/acri/2024/v24i6814
B. Manjunatha., Appaji Pundalik Naik, K. R. Mahendra, M. S., G. H., N. R. Kiran, Damodhara G. N., Karthik R.
Multi-Dimensional Scaling (MDS) is a data visualization method that identifies clusters of points by representing the distances or dissimilarities between sets of objects in a lower-dimensional space. This paper explores the theoretical concepts of MDS, various methods of implementation, and the analytical processes involved. Emphasis is placed on the "Stress" function, a goodness-of-fit metric that quantifies the discrepancy between distances in high-dimensional and lower-dimensional spaces. Practical examples and detailed procedures for implementing MDS using MS-Excel and R are provided to enhance understanding. The paper also discusses the use of Scree-plots for determining the optimal number of dimensions. Applications of MDS in different fields, including marketing, ecology, molecular biology, and social networks, are presented with examples on Perceptions of Nations data and Morse code confusion data. Additionally, as a significant contribution, a case study on factors affecting agricultural productivity is included. The versatility and utility of MDS in simplifying complex data and facilitating better decision-making are demonstrated through these practical applications and software implementations.
多维缩放(MDS)是一种数据可视化方法,它通过表示低维空间中对象集之间的距离或差异来识别点群。本文探讨了 MDS 的理论概念、各种实现方法以及相关的分析过程。重点放在 "Stress "函数上,这是一个拟合度量,用于量化高维空间和低维空间中的距离差异。文中提供了使用 MS-Excel 和 R 实现 MDS 的实用示例和详细步骤,以加深理解。本文还讨论了如何使用屏幕图确定最佳维数。文章介绍了 MDS 在不同领域的应用,包括市场营销、生态学、分子生物学和社交网络,并以 "对国家的看法 "数据和莫尔斯电码混淆数据为例进行了说明。此外,作为一项重要贡献,还包括一项关于影响农业生产力因素的案例研究。通过这些实际应用和软件实现,展示了 MDS 在简化复杂数据和促进更好决策方面的多功能性和实用性。
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引用次数: 0
The Influence of Socio-demographic Variables on Coping Strategies for Stress and Depression among Lecturers in Selected Universities of Ogun State, Nigeria 尼日利亚奥贡州部分大学讲师的社会人口变量对压力和抑郁应对策略的影响
Pub Date : 2024-08-09 DOI: 10.9734/acri/2024/v24i6812
Oluwaseyi Bewaji, O. Ekeagwu
Aim: This research investigated the relationship between socio-demographic factors and coping mechanisms for stress and depression among lecturers in selected universities in Ogun State, Nigeria. Sample: A sample population of 285 lecturers from three universities participated in the study. Place and Duration: The study was conducted at three purposively selected universities in Ogun State, Nigeria. Methodology: A total of 285 lecturers participated in the study, representing a response rate of 92%. Data were collected using questionnaires distributed physically and online. Socio-demographic variables such as age, gender, educational level, type of university, academic rank, and years of service were assessed, alongside coping mechanisms for stress and depression. Results: Analysis revealed a diverse range of socio-demographic characteristics within the sample. The study found a moderate utilization of adaptive coping mechanisms for stress and depression, while the prevalence of maladaptive coping strategies remained low. Significant correlations emerged between socio-demographic variables such as age, gender, education level, and academic rank, and both adaptive and maladaptive coping strategies for stress. Additionally, age and gender demonstrated significant associations with coping strategies for depression. Conclusion: These findings underscored the pivotal role of socio-demographic factors in shaping the frequency and nature of coping mechanisms adopted by lecturers. Implications for the design of targeted support interventions within academic environments were discussed. Recommendations: Universities should prioritize implementing policies and training programs that promote adaptive coping mechanisms for stress and depression among lecturers, fostering a supportive environment that enhances their well-being, resilience, and professional effectiveness.
目的:本研究调查了尼日利亚奥贡州部分大学讲师的社会人口因素与压力和抑郁应对机制之间的关系。样本:来自三所大学的 285 名讲师参与了研究。地点和时间:研究在尼日利亚奥贡州三所特定大学进行。研究方法:共有 285 名讲师参与了研究,回复率为 92%。数据收集采用了实际发放和在线发放问卷的方式。除了评估压力和抑郁的应对机制外,还评估了年龄、性别、教育水平、大学类型、学术级别和工作年限等社会人口变量。结果显示分析表明,样本的社会人口特征多种多样。研究发现,适度使用适应性压力和抑郁应对机制的比例较高,而不适应性应对策略的比例仍然较低。年龄、性别、教育水平和学术排名等社会人口变量与压力的适应性应对策略和适应性不良应对策略之间存在明显的相关性。此外,年龄和性别与抑郁的应对策略也有显著关联。结论这些发现强调了社会人口因素在影响讲师所采用的应对机制的频率和性质方面所起的关键作用。讨论了在学术环境中设计有针对性的支持干预措施的意义。建议大学应优先实施促进讲师对压力和抑郁的适应性应对机制的政策和培训计划,营造一种支持性环境,以提高他们的幸福感、复原力和专业效率。
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引用次数: 0
Financial Reporting Revolution: How it Integration Drives Efficiency and Accuracy 财务报告革命:整合如何提高效率和准确性
Pub Date : 2024-08-08 DOI: 10.9734/acri/2024/v24i6811
T. Kipilimba
Aims: This study investigated the transformative impact of IT integration on financial reporting, examining how organizations leverage advanced technologies to streamline operations, enhance data accuracy, and improve strategic decision-making. Methodology: Through a quantitative analysis of 100 respondents representing diverse sectors, including manufacturing, finance, healthcare, IT, retail, and others, this research explored key indicators such as reduction in manual errors, improvement in coordination and collaboration, and effectiveness of training programs post-IT integration. Results: Findings revealed a significant reduction in manual errors, with 60% of respondents reporting a notable decrease, attributing it to automated data validation and streamlined workflows. Moreover, 40% of participants note a significant improvement in coordination and collaboration across departments, facilitated by centralized data access and real-time communication tools. The study also highlighted the effectiveness of training initiatives, with 75% of respondents indicating moderate to very effective outcomes in preparing teams to utilize new IT systems proficiently. Despite these advancements, challenges such as system integration complexities and varying levels of user satisfaction remain prevalent. Approximately 15% of respondents reported no significant change in operational efficiency post-IT integration, citing issues related to data integration and system compatibility. Furthermore, while 45% expressed satisfaction with IT integration, 10% remained neutral or dissatisfied, emphasizing the importance of ongoing support and continuous improvement in maximizing the benefits of IT integration. Conclusion: The integration of IT systems has significantly enhanced efficiency and accuracy in financial reporting across various industries, as evidenced by the majority of respondents who reported improvements in areas such as time efficiency, cost effectiveness, accuracy of reporting, and strategic decision-making. Real-time reporting capabilities enabled by advanced IT systems have empowered organizations to respond swiftly to market changes, optimize resource allocation, and make informed strategic decisions.
目的:本研究调查了信息技术整合对财务报告的变革性影响,探讨了组织如何利用先进技术来简化运营、提高数据准确性并改进战略决策。研究方法:通过对代表不同行业(包括制造业、金融业、医疗保健业、IT 业、零售业等)的 100 名受访者进行定量分析,本研究探讨了 IT 整合后减少人工错误、改善协调与合作以及培训计划有效性等关键指标。研究结果研究结果显示,人工错误大幅减少,60% 的受访者表示明显减少,这归功于自动化数据验证和简化的工作流程。此外,40% 的受访者指出,集中数据访问和实时通信工具促进了跨部门协调与合作的显著改善。研究还强调了培训计划的有效性,75% 的受访者表示在帮助团队熟练使用新 IT 系统方面取得了中等至非常有效的成果。尽管取得了这些进步,但系统集成复杂性和用户满意度参差不齐等挑战依然普遍存在。约 15%的受访者表示,IT 整合后的运营效率没有明显变化,原因是存在与数据整合和系统兼容性相关的问题。此外,虽然 45% 的受访者对 IT 整合表示满意,但仍有 10% 的受访者持中立或不满意态度,这强调了持续支持和不断改进对于最大限度地发挥 IT 整合效益的重要性。结论大多数受访者表示在时间效率、成本效益、报告准确性和战略决策等方面都有所改进,由此可见,IT 系统集成大大提高了各行各业财务报告的效率和准确性。先进的 IT 系统带来的实时报告能力使企业能够迅速应对市场变化、优化资源配置并做出明智的战略决策。
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引用次数: 0
Analysis of Socio-economic Impacts of COVID-19 on Agricultural Practices Using Stepwise Regression 利用逐步回归法分析 COVID-19 对农业实践的社会经济影响
Pub Date : 2024-05-23 DOI: 10.9734/acri/2024/v24i5716
Manju Prem S., Mohanraj M., Sneha M. A., Swadhin Priyadarsinee, Pooja Krishna J.
The COVID-19 pandemic has profoundly impacted rural farming communities, revealing significant shifts in economic factors, production methods, and farm management practices. This study, conducted in Chikkaballapur district, Karnataka, delves into the socio-economic ramifications of the pandemic on agricultural practices and the livelihoods of farmers. Utilizing a stepwise regression analysis approach, the research identifies influential variables such as changes in farm management, production, and health and sanitation facilities. Data were collected from 50 respondents during the pandemic's peak, employing statistical measures like mean, standard deviation, and coefficient of variation for comprehensive analysis. The findings underscore the intricate relationships between various factors affecting rural livelihoods and highlight the need for targeted interventions and policy measures. The study emphasizes the critical need for tailored policy interventions to address the socio-economic impacts of COVID-19 on agricultural practices. Specifically, policy recommendations include enhancing access to agricultural extension services, promoting digital agricultural solutions, investing in healthcare infrastructure, supporting economic diversification, and strengthening social protection mechanisms. By aligning policy interventions with the findings and indicators observed in the research, policymakers can effectively support rural farming communities in navigating the challenges posed by the pandemic and building resilience for the future.
COVID-19 大流行对农村农业社区产生了深远影响,揭示了经济因素、生产方式和农场管理方法的重大转变。本研究在卡纳塔克邦 Chikkaballapur 地区进行,探讨了大流行病对农业生产方式和农民生计的社会经济影响。研究采用逐步回归分析方法,确定了农场管理、生产以及健康和卫生设施的变化等有影响的变量。研究在疫情高峰期收集了 50 名受访者的数据,并采用平均值、标准差和变异系数等统计指标进行综合分析。研究结果强调了影响农村生计的各种因素之间错综复杂的关系,并突出了采取有针对性的干预措施和政策措施的必要性。研究强调,亟需采取有针对性的政策干预措施,以应对 COVID-19 对农业生产的社会经济影响。具体而言,政策建议包括加强农业推广服务、推广数字农业解决方案、投资医疗保健基础设施、支持经济多样化以及加强社会保护机制。通过将政策干预与研究中观察到的发现和指标相结合,政策制定者可以有效地支持农村农业社区应对疫情带来的挑战,并为未来建设抗灾能力。
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引用次数: 0
Exploring the Socioeconomic Dynamics and Technological Adaptation among Farmers: Implications for Sustainable Agricultural Development and Rural Livelihoods in Jaipur, India 探索农民的社会经济动态和技术适应性:对印度斋浦尔可持续农业发展和农村生计的影响
Pub Date : 2024-05-23 DOI: 10.9734/acri/2024/v24i5714
Meenakshi Jakhar, R. N. Sharma, B.S. Badhala, Jhabar Mal Tetarwal
The research was conducted in Jaipur district of Rajasthan. There are 23 AAO departments under the jurisdiction of Deputy Commissioner of Agriculture, Jothwara (Jaipur). Among these, 4 AAO departments viz. Hingonia, Jobner, Boraj and Bichun were deliberately chosen for this study because the AAO circle is next to SKNAU, Jobner is within a radius of 20 km, researchers from the school respectively many people are making technological improvements in nearby villages and farmers are also active. A total of 120 participants were selected from these four AAO circles. Judging from the entire health history of farmers benefiting from Nongtang, the majority of farmers benefiting from Nongtang are middle-aged (35 to 51 years old) (58.33%). Primary school graduates (20.00%), farmers are union members (37.50%), their share of average annual income (77.50%) is Rs. 1,26,781 to Rs. 4,72,303 and farmers share small land. category (38.33%). Moreover, most of the beneficiary farmers (44.17%) have moderate farming experience ranging from 15 to 31 years, (23.19%) farmers use PM Kisan application, (70.00%) farmers use information technology, (68%) 34) consider agriculture as the main business. job. The relationship between income and technological change shows that there is a positive relationship between education level, social participation, annual income, insured land, agriculture, agricultural practice and knowledge. The beneficiary's social profile indicates education, community involvement, annual income, land ownership, farm practice use, and data-effective and relevant job creation and annual income. The correlation between the profile of the beneficiaries and their social variables shows a positive relationship between the level of education, social participation, and annual income. There is a significant relationship between the use of agricultural practices and the change in personal property and the education level of family members. Agricultural knowledge and information are important and positively related to changes in household members' education levels and ownership of agricultural equipment.
研究在拉贾斯坦邦的斋浦尔地区进行。乔特瓦拉(斋浦尔)农业副专员辖下有 23 个 AAO 部门。本研究特意选择了其中的 4 个 AAO 部门,即 Hingonia、Jobner、Boraj 和 Bichun,因为这 4 个 AAO 圈毗邻斯堪的纳维亚大学,Jobner 半径在 20 公里以内,学校的研究人员分别在附近村庄的许多人和农民中开展技术改进活动。这四个 AAO 圈共选出了 120 名参与者。从农堂受益农民的整个健康史来看,中年(35 至 51 岁)农民占大多数(58.33%)。小学毕业生(20.00%),农民是工会成员(37.50%),他们的平均年收入(77.50%)在 1,26,781 至 4,72,303 卢比之间,农民拥有小块土地(38.33%)。此外,大多数受益农户(44.17%)拥有 15 至 31 年不等的中等耕作经验,(23.19%)农户使用 PM Kisan 应用程序,(70.00%)农户使用信息技术,(68%)34)将农业视为主业。收入与技术变革之间的关系表明,教育水平、社会参与、年收入、投保土地、农业、农业 实践和知识之间存在正相关关系。受益人的社会概况表明教育程度、社区参与、年收入、土地所有权、农业实践的使用以及数据有效和相关的工作岗位创造和年收入。受益人概况与其社会变量之间的相关性表明,教育水平、社会参与和年收入之间存在正相关关系。农业实践的使用和个人财产的变化与家庭成员的教育水平之间存在重要关系。农业知识和信息非常重要,与家庭成员教育水平和农业设备所有权的变化呈正相关。
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引用次数: 0
Marketing Efficiency in Cut Roses: A Comparative Study of Organised Vis-A-Vis Unorganised Markets 切花玫瑰的营销效率:有组织市场与无组织市场的比较研究
Pub Date : 2024-05-21 DOI: 10.9734/acri/2024/v24i5713
Vidhyashree, H.S., S. K. M., Ganeshagouda I. Patil, C. G. Yadava, Gagana, M. D., Ramappa, K. B, Srinivasalu G. B., .. Raghavendra
The present study is mainly about the comparative analysis of marketing efficiency of cut roses under organised and unorganised markets. For the present study, the primary data was collected from the Krishna Raja Market, Bengaluru an unorganised market, whereas the International Flower Auction Centre, Bengaluru (IFAB) is an organised market. Data collected from 40 market intermediaries in each market structure and total intermediaries were to be 80. Forty rose vendors are randomly selected to gather marketing constraints in organised market. In the study area, three major marketing channels were observed for cut rose marketing in unorganised market structure: Channel I: Producer – Wholesaler – Retailer – Consumer; Channel II: Producer – Retailer – Consumer; and Channel III: Producer– Commission agent– Retailer – Consumer, whereas in the organised market, two marketing channels were prominent, i.e., Channel I: Producer – IFAB – Wholesaler – Retailer – Consumer and Channel II: Producer – IFAB – Retailer – Consumer. Marketing efficiency was estimated using the Acharya and Agarwal methodology. In this analysis, direct marketing channel was found to be the most efficient for unorganised and organised markets. Marketing constraints were analysed using Rank Based Quotient (RBQ) technique. Quality standards and the lack of price information were the most important problems for the farmers while marketing the flowers in organised markets rather than unorganised markets. Hence, policymakers should provide producers with the required facilities and impart proper knowledge about quality standards to farmers, leading to more income for producers.
本研究主要是对有组织市场和无组织市场中切花玫瑰的营销效率进行比较分析。本研究从班加罗尔的克里希纳拉贾市场(Krishna Raja Market)和班加罗尔的国际花卉拍卖中心(International Flower Auction Centre, Bengaluru, IFAB)收集原始数据,前者是一个无组织市场,后者是一个有组织市场。从每个市场结构中的 40 个市场中介收集数据,中介总数为 80 个。随机抽取了 40 个玫瑰商贩,以收集有组织市场的营销制约因素。在研究地区,无组织市场结构中的切花玫瑰营销主要有三种营销渠道:渠道一:生产商-批发商-零售商-消费者;渠道二:生产商-零售商-消费者;渠道三:生产商-批发商-零售商-消费者:而在有组织市场上,两个营销渠道非常突出,即渠道 I:生产商 - IFAB - 批发商 - 零售商 - 消费者和渠道 II:生产商 - IFAB - 批发商 - 零售商 - 消费者:生产商 - IFAB - 零售商 - 消费者。营销效率采用 Acharya 和 Agarwal 方法进行估算。在这项分析中,直销渠道被认为是无组织市场和有组织市场中效率最高的渠道。使用排名商数(RBQ)技术分析了营销制约因素。质量标准和缺乏价格信息是花农在有组织市场而非无组织市场销售花卉时面临的最重要问题。因此,决策者应为生产者提供所需的设施,并向农民传授有关质量标准的正确知识,从而增加生产者的收入。
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引用次数: 0
Pathogenic Level of Reniform Nematode (Rotylenchulus reniformis Linford & Oliveira, 1940) on Tomato (Solanum lycopersicum L.) 番茄(Solanum lycopersicum L.)上肾形线虫(Rotylenchulus reniformis Linford & Oliveira, 1940)的致病水平
Pub Date : 2024-05-19 DOI: 10.9734/acri/2024/v24i5712
Prerana Bhuyan, Gitanjali Devi
Tomato is an important vegetable crop. Various plant parasitic nematode cause extensive damage during growth stage of tomato. Pathogenic level of Reniform nematode (Rotylenchulus reniformis), was investigated on tomato var. Pusa Ruby by inoculating 10, 100, 500, 1000, 5000 and 10000 nematodes per kg autoclaved soil. Observations on plant growth parameters and nematode numbers were recorded at 60 days following inoculation. The inoculated seedlings showed a progressive decrease in the plant growth parameters with increase in inoculum level of R. reniformis. Significant reduction in plant growth parameters were recorded at 1000 and above nematodes/kg soil. There was a gradual increase in the number of females, egg masses per root system, and nematode population of R. reniformis with increase in inoculum level. The rate of multiplication decreased with increase in the level of inoculation. The pathogenic level of R. reniformis was found to be 1000 nematodes per kg soil.
番茄是一种重要的蔬菜作物。在番茄的生长阶段,各种植物寄生线虫会对其造成广泛的损害。通过每公斤高压灭菌土壤接种 10、100、500、1000、5000 和 10000 条线虫,研究了肾形线虫(Rotylenchulus reniformis)对番茄变种 Pusa Ruby 的致病水平。接种后 60 天,观察植物生长参数和线虫数量。接种秧苗的植物生长参数随着 R. reniformis 接种量的增加而逐渐下降。每千克土壤中线虫数达到 1000 或以上时,植物生长参数显著下降。随着接种量的增加,雌线虫数量、每个根系的卵块数量以及肾形线虫的数量也逐渐增加。繁殖率随着接种量的增加而降低。发现肾形线虫的致病水平为每公斤土壤 1000 条线虫。
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引用次数: 0
Effect of Milk Sources on Whey Protein and Fractions of Casein 牛奶来源对乳清蛋白和酪蛋白组分的影响
Pub Date : 2024-05-16 DOI: 10.9734/acri/2024/v24i5711
R. M, H. M. Jayaprakasha, .. Akshaykumar, Nagamani A
Aim: Milk from Cow, Buffalo and goat was used for the study of isolation and fractionation of casein and whey proteins. Buffalo and goat milk were subjected to physicochemical attribute studies and a detailed protein profile namely total protein, casein fractions α-, β- and κ casein and whey protein were separated by urea fractionation method. The aim of this study was to compare various casein fractions isolated from cows, Buffalo and Goat milk. The method of separating casein into its fractions is based on the solubility of the individual components in urea the solution. The separation of casein fractions was carried out in dissolving molar concentration of urea and the yield of fractions and total protein percentages were calculated. Buffalo and goat milk have comparatively higher total protein and whey protein content as compared to cow milk. Maximum whey proteins found in goat milk (20.58%) whereas cow milk had the lowest whey protein (19.29%). Among casein fractions, α fraction (16.64%) was found maximum in Buffalo milk followed by cow (14.92%) and goat milk (5.42%). The goat milk showed the highest level of β casein (17.81%) whereas, the lowest concentration of β casein was found in cow milk (9.38%). Study Design: A significant contribution to the total milk production of India comes from buffalo milk and goat milk. Despite that, Buffalo milk and goat milk are not being utilized for many products because of difference in composition of different species milk and their inherent problems associated in the production of products. Place and Duration of Study: Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Dairy Science College, Hebbal, Bangalore Karnataka, India Materials: The Goat milk samples were collected from Sinchana Goat and Sheep farm, Marenahalli village (Bengaluru Rural Dist) and Buffalo milk was obtained from Country Delight Pvt. Ltd., J. P. Nagar, Bengaluru, Karnataka. Cow milk used in this investigation was collected from SEDP, Dairy Science College, Hebbal, Bengalore.. All the glassware used was soaked in chromic acid solution, repeatedly washed with water, rinsed with distilled water and dried before use. For microbiological analysis dried test tubes, conical flasks, pipettes were cotton plugged and sterilized in a hot air oven. The chemicals and reagents used in this study were mainly of analytical grade procured from Prince Laboratory Company Pvt. Limited, Bangalore. The protein molecular weight markers used for the electrophoretic study were procured from Bangalore Genei Pvt. Ltd.  Methodology: Standard urea fractionation method adopted by Hipp et al was used for isolation and fractionation of caseins. Skim milk was prepared by subjecting whole milk for centrifugation, the skim milk obtained was acidified using 1 % hydrochloric acid at 200 C to obtain casein precipitate. Obtained whole casein was dissolved in 3.3 M urea at pH 7.5 and adjusted to pH 4.6 which precipitates the bulk of α casein and l
目的:使用牛奶、水牛奶和山羊奶研究酪蛋白和乳清蛋白的分离和分馏。对水牛和山羊奶进行了理化属性研究,并通过尿素分馏法分离出了详细的蛋白质谱,即总蛋白质、α-、β-和κ酪蛋白以及乳清蛋白。这项研究的目的是比较从牛奶、水牛奶和山羊奶中分离出来的各种酪蛋白组分。将酪蛋白分离成酪蛋白组分的方法是基于各组分在尿素溶液中的溶解度。酪蛋白馏分的分离是在溶解摩尔浓度的尿素中进行的,并计算了馏分的产量和总蛋白质的百分比。与牛奶相比,水牛奶和山羊奶的总蛋白和乳清蛋白含量相对较高。山羊奶的乳清蛋白含量最高(20.58%),而牛奶的乳清蛋白含量最低(19.29%)。在酪蛋白组分中,水牛奶的α组分(16.64%)最高,其次是牛奶(14.92%)和山羊奶(5.42%)。山羊奶中的β酪蛋白含量最高(17.81%),而牛奶中的β酪蛋白含量最低(9.38%)。研究设计:印度牛奶总产量的很大一部分来自水牛奶和山羊奶。尽管如此,水牛奶和山羊奶并没有被用于生产许多产品,因为不同种类牛奶的成分不同,而且在产品生产过程中存在固有的问题。研究地点和时间:印度班加罗尔卡纳塔克邦 Hebbal 的卡纳塔克兽医、动物和渔业科学大学(KVAFSU)乳品科学学院 材料:山羊奶样品采集自 Marenahalli 村(班加罗尔农村区)的 Sinchana 山羊和绵羊农场,水牛奶样品采集自卡纳塔克邦班加罗尔 J. P. Nagar 的 Country Delight Pvt.本研究中使用的牛奶取自本加洛尔希伯尔的乳品科学学院 SEDP。使用前,所有玻璃器皿都浸泡在铬酸溶液中,用水反复清洗,再用蒸馏水冲洗并晾干。为了进行微生物分析,干燥的试管、锥形瓶和移液管都用棉塞塞好,并在热风炉中消毒。本研究使用的化学品和试剂主要来自班加罗尔的 Prince Laboratory Company Pvt.电泳研究中使用的蛋白质分子量标记从班加罗尔 Genei Pvt. 方法:采用 Hipp 等人的标准尿素分馏法分离和分馏酪蛋白。脱脂牛奶的制备方法是将全脂牛奶离心后,用 1 % 的盐酸在 200 摄氏度下酸化脱脂牛奶,以获得酪蛋白沉淀。将得到的全酪蛋白溶解在 pH 值为 7.5 的 3.3 M 尿素中,然后调节 pH 值至 4.6,这样大部分 α 酪蛋白就会沉淀下来,剩下的 k 酪蛋白成为可溶性滤液。再次将上清液调节至 pH 4.9,稀释至 1.0 M 尿素,并加热至 300 C,使 β-酪蛋白沉淀。然后,加入硫酸铵从沉淀中分离出 k-酪蛋白。 结果蛋白质来源对不同物种的总酪蛋白、酪蛋白组分和乳清蛋白含量有显著影响。水牛奶的总酪蛋白(34.30 克/升)和乳清蛋白(8.87 克/升)含量最高,其次是牛奶(28.52 克/升),山羊奶最低(28.45 克/升)。总蛋白、酪蛋白和乳清蛋白含量受奶源的影响很大。结论牛奶的酪蛋白产量最高(54.31%),高于水牛奶(48.95%),山羊奶的产量较低。在三种牛奶中,山羊奶的 β 酪蛋白含量最高(54.05%),其次是水牛奶(36.03%)和牛奶(34.14%)。建议:Rasagulla 将被全球所有年龄组的人普遍饮用。因此,饮用 Rasagulla 有助于满足整体营养需求。不过,这项研究是在试验规模上进行的。此外,商业化生产可能需要花费巨额成本对蛋白质和分离肽进行酶改性,以实现增值。
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