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An analytical framework to measure the social return of community-supported agriculture 衡量社区支持农业社会回报的分析框架
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-18 DOI: 10.1080/21683565.2023.2236989
M. Medici, M. Canavari, A. Castellini
ABSTRACT The lack of objective sustainability indicators and measurements leaves the performance of agricultural systems under-valued, limiting the possibility of communicating any evidence about promoting economic, social, and environmental values to food system stakeholders. In this study, a cost-benefit analysis based on the social return on investment (SROI) was designed and applied to five community-supported agriculture (CSA) initiatives. CSA are local food supply chains that have become popular in recent years, characterized by features such as member volunteer commitment, reduction of synthetic inputs in food production, and shortening of the supply chain to reduce costs and environmental impact. Social and environmental outcomes related to the wider spread of impacts were comprehensively assessed in the study. A participatory assessment with CSA representatives resulted in the incorporation of several ecosystem service (ES) indicators identified from the literature. Findings revealed that the case studies analyzed are characterized by a limited but positive socio-environmental performance compared to conventional food supply chains, thereby demonstrating that they can create real value in terms of social gains and mitigation of environmental impacts.
摘要:由于缺乏客观的可持续性指标和衡量标准,农业系统的绩效被低估,限制了向粮食系统利益相关者传达任何关于促进经济、社会和环境价值的证据的可能性。在本研究中,设计了一个基于社会投资回报率(SROI)的成本效益分析,并将其应用于五个社区支持农业(CSA)倡议。CSA是近年来流行的当地食品供应链,其特点是成员志愿者承诺、减少食品生产中的合成投入以及缩短供应链以降低成本和环境影响。该研究全面评估了与更广泛影响相关的社会和环境结果。CSA代表的参与性评估纳入了文献中确定的几个生态系统服务指标。调查结果显示,与传统食品供应链相比,所分析的案例研究的特点是有限但积极的社会环境表现,从而表明它们可以在社会收益和减轻环境影响方面创造真正的价值。
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引用次数: 0
Is there an agroecology movement in the USA? 美国有农业生态学运动吗?
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-14 DOI: 10.1080/21683565.2023.2233787
S. Gliessman
This question was uppermost in the minds of the 100 participants at the recent “Agroecology Summit” held May 23-25, 2023, at the Elms Center in Excelsior Springs, Missouri, outside of Kansas City. This is also a question I have asked myself many times in my role as Editor of Agroecology and Sustainable Food Systems, and wondering why such a small percentage of the papers submitted to our journal comes from agroecological research carried out in the USA. The Summit was organized to address this question – are we a movement? The key concept that drove the organization of the Summit is also a key concept of agroecology -diversity. Diversification through the process of cocreation of knowledge coming from multiple stakeholders in the food system in order to create a diverse pathway for the scientific field of agroecology to lead/follow. Diversification through integrating disciplines, approaches, ideologies, belief system, and cultures to achieve true transdisciplinarity. Diversification through many voices identifying priorities in agroecology so we can move beyond isolation and achieve full collaboration in the movement. Diversification through the transformation of food systems in the USA that respects multiple visions, beliefs, cultures, goals, and dreams. All of these “ways of knowing” and “ways of being” contributed to bringing together a remarkable diversity of participants. Through a process of an opening keynote presentation, panel discussions, break-out groups, report backs of key ideas, and wrap-up discussions, a dynamic and evolving vision of the current status and future development of agroecology in the USA took shape. The keynote and four panels included the following:
在最近于2023年5月23日至25日在堪萨斯城外密苏里州Excelsior Springs的Elms中心举行的“农业生态学峰会”上,100名与会者最关心的问题是这个问题。这也是我在担任《农业生态学与可持续粮食系统》杂志编辑期间多次问自己的一个问题,我想知道为什么提交给我们杂志的论文中只有这么小的一部分来自美国的农业生态学研究。这次峰会是为了解决这个问题而组织的——我们是一场运动吗?推动首脑会议组织的关键概念也是农业生态学的一个关键概念——多样性。通过共同创造来自食品系统中多个利益相关者的知识的过程实现多样化,为农业生态学科学领域的领导/遵循创造一条多样化的途径。通过整合学科、方法、意识形态、信仰体系和文化实现多样化,以实现真正的跨学科性。通过多种声音确定农业生态学的优先事项,使我们能够超越孤立,在运动中实现充分合作。通过转变美国的食品系统实现多样化,尊重多种愿景、信仰、文化、目标和梦想。所有这些“认识的方式”和“存在的方式”都有助于将各种各样的参与者聚集在一起。通过开幕式主题演讲、小组讨论、分组讨论、关键思想报告和总结讨论,形成了对美国农业生态学现状和未来发展的动态和不断发展的愿景。主题演讲和四个小组讨论包括以下内容:
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引用次数: 0
Quality characteristics of milk ice cream with citrus fibers and gum 含柑橘纤维和树胶的牛奶冰淇淋的品质特性
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-261-268
A. Landikhovskaya, A. Tvorogova
Currently at least 4 food additives — emulsifiers and hydrocolloids — are used for ice cream production to stabilize its structure. However adding these substances reduces the demand for this product among healthy lifestyle adepts. The aim of the research was to define the possibility of using citrus fibers and their compositions together with guar and xanthan gums to stabilize the milk ice cream structure. Samples with gelatin and a complex stabilizer (emulsifier) were used as control samples. It was established that adding of citrus fibers in amount of 0.6% makes the product bitter, does not provide the required level of dynamic viscosity of the mixture, it forms an unstable air phase and large ice crystals. The combination of dietary fibers with gums had a positive effect on these quality characteristics. The highest dispersion of ice crystals (average size accounted for 36–39 μm) and dynamic viscosity of the mixture, comparable with the quality characteristics of the sample with a complex stabilizeremulsifier, was achieved in the sample with added fibers and xanthan gum. The sample with dietary citrus fiber and guar gum showed better characteristics in terms of heat resistance in comparison with the samples with fiber only, as well as samples with plain fiber and guar gum. After 60 min of thermostating, the mass fraction of melt was equal to 7%, which is 2.8 and 2 times less than in samples without gums and with xanthan gum. The correlation was found between the parameter “hardness” and the parameter “thermal stability”: the hardest samples showed the highest resistance to melting. Based on the results of the research, the expediency of using combinations of citrus fibers and guar gum or xanthan gum in the production of ice cream with a limited number of food additives was established. Meanwhile it is necessary to take into account the state of structural elements during the product storage, and using the packaging that helps preserve the shape of the ice cream portion.
目前,至少有4种食品添加剂——乳化剂和水胶体——用于冰淇淋生产,以稳定其结构。然而,添加这些物质会减少健康生活习惯者对该产品的需求。这项研究的目的是确定使用柑橘纤维及其成分与瓜尔胶和黄原胶一起稳定牛奶冰淇淋结构的可能性。用明胶和复合稳定剂(乳化剂)作为对照样品。结果表明,柑橘纤维添加量为0.6%时,产品苦味明显,混合液的动态粘度不能满足要求,气相不稳定,形成较大的冰晶。膳食纤维与树胶的组合对这些品质特性有积极的影响。在添加纤维和黄原胶的情况下,样品的冰晶分散度最高(平均粒径为36 ~ 39 μm),动态粘度与添加复合稳定乳化剂的样品的质量特性相当。添加柑橘纤维和瓜尔胶的样品在耐热性方面优于只添加纤维的样品,也优于添加普通纤维和瓜尔胶的样品。保温60 min后,熔体质量分数为7%,分别比不加胶和加黄原胶的样品低2.8倍和2倍。“硬度”参数与“热稳定性”参数之间存在相关性:最硬的样品具有最高的抗熔化性。在此基础上,确定了柑橘纤维与瓜尔胶或黄原胶复合使用在有限食品添加剂条件下生产冰淇淋的便利性。同时,在产品储存过程中,必须考虑结构元件的状态,并使用有助于保持冰淇淋部分形状的包装。
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引用次数: 0
Role of feed adaptogens in forming the concept of organic pig production 饲料适应原在生猪有机生产理念形成中的作用
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-255-260
R. Nekrasov, N. Bogolyubova, M. Chabaev, K. Ostrenko, R. Rykov, A. Semenova, V. Nasonova
One of the priorities of the Strategy of scientific and technological development of the Russian Federation (approved by Presidential Decree № 642 of December 1, 2016) is the transition to a highly productive and environmentally friendly agriculture. Organic agriculture can help to ensure sufficient food for the population by 2050 and simultaneously reduce the impact of agriculture on the environment only if it is implemented as part of a system of agricultural production, which should provide the same volume and composition of products as in the baseline scenario. An important aspect of organic livestock production is to reduce the impact of stress factors on animals. A promising solution can be the use of natural biologically active substances with pronounced antioxidant properties. The action of natural bioflavonoids (Taxifolin) and also in a complex with vitamins on the organism of fattening pigs during the periods of stress of various etiology (climatic, fodder, social etc.) has been studied. A complex including vitamin C, vitamin E and Taxifolin has been developed. The provisions of the method of feeding pigs as a way to reduce the negative impact of stress factors on meat quality by means of the studied nutritional factor have been studied experimentally.
俄罗斯联邦科技发展战略(2016年12月1日第642号总统令批准)的优先事项之一是向高产和环境友好型农业过渡。有机农业只有作为农业生产系统的一部分加以实施,才能帮助确保到2050年为人口提供足够的粮食,同时减少农业对环境的影响,而农业生产系统应提供与基线情景相同的产品数量和组成。有机畜牧生产的一个重要方面是减少应激因素对动物的影响。一个有希望的解决方案是使用具有明显抗氧化特性的天然生物活性物质。本文研究了天然生物黄酮类化合物(Taxifolin)及其复合维生素在各种原因(气候、饲料、社会等)应激期间对育肥猪机体的作用。一种包括维生素C、维生素E和紫杉醇的复合物已经被开发出来。试验研究了利用所研究的营养因子来降低应激因子对肉品质的负面影响的猪饲养方法的规定。
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引用次数: 0
Examination of the extract from lichen (Cetraria islandica) after an ultrasonic impact 超声波冲击后地衣提取物的检测
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-171-179
K. Nitsievskaya, S. Stankevich, E. Boroday
An integral part of human healthy nutrition is intake of biologically active substances. Extraction is an important stage of isolation of different bioactive substances from plants and effectiveness of their extraction increases with the use of ultrasonic treatment. The aim of this research was to study an effect of different types of aqueous media and treatment methods on Cetraria islandica, including the use of principles of ultrasonic treatment and maceration methods (infusion). The experimental studies consisted in detecting an effect of the active acidity of the aqueous medium (solvent) and method of impact upon extraction on several physicochemical and rheological indicators. The experimental samples of extracts were examined by the following indicators: active acidity and redox potential detected by the ionometric technique; dynamic viscosity and the depth of output of polymeric forms of phenolic substances determined by measuring the optical density and transmission coefficient. Mathematical processing of data using the regression analysis was carried out. The results are presented as graphs, tables and regression equations. It has been established that transfer of substances from Cetraria islandica to its extracts had high results at pH = 7.0 and рН = 9.0 of the solvent. When obtaining extracts by the infusion method, the quantity of polymeric compounds in the transition process was 1.3 times higher than the quantity of monomeric forms of phenolic compounds. The highest value of the dynamic viscosity was revealed in the extract sample obtained by the infusion method in the alkaline medium. The extracts obtained can be used in food system modeling.
人体健康营养的一个组成部分是摄入生物活性物质。提取是从植物中分离不同生物活性物质的重要步骤,超声处理的应用提高了提取活性物质的有效性。本研究的目的是研究不同类型的水介质和处理方法对岛天竺鼠的影响,包括超声波处理原理和浸渍方法(输液)的使用。实验研究包括检测水介质(溶剂)的活性酸度对几种物理化学和流变性指标的影响以及对提取的影响方法。采用离子滴定法测定活性酸度和氧化还原电位;通过测量光密度和透射系数来确定酚醛物质聚合形式的动态粘度和输出深度。利用回归分析对数据进行数学处理。结果以图表、表格和回归方程的形式呈现。在pH = 7.0和рН = 9.0的溶剂条件下,从天竺葵到其提取物的物质转移率较高。用输注法提取提取物时,过渡过程中高分子化合物的含量是单体酚类化合物含量的1.3倍。在碱性培养基中,用输注法得到的提取液样品的动态黏度最高。所得提取物可用于食品系统建模。
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引用次数: 0
Individual integrated approach to honey identification using instrumental methods of analysis and statistical processing of results 使用仪器分析和统计处理结果的单个蜂蜜鉴定综合方法
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-211-223
A. Panasyuk, E. Kuzmina, D. Sviridov, M. Ganin
Bee honey is a valuable highly nutritive natural product; it is widely consumed among the population. Due to its high cost the natural honey often becomes the object of adulteration. The authenticity of honey is the most important criterion of quality, as on the one hand it ensures the biosecurity of honey, and provides the healthy market competition on the other hand. In this regard, the issue of honey identification is quite acute in all countries with developed beekeeping culture. The authors provide an overview of domestic and foreign regulatory documents regarding the authenticity of honey, as well as its status as a product with a controlled designation of origin. Based on the analysis of scientific literature, the most significant studies aimed to a method of honey authenticity confirmation were selected and brought out. These studies were carried out in the countries of the European Union, China, Brazil, the USA, Mexico and other countries. These studies showed that chromatographic methods and the method of isotope mass spectrometry are the most effective for detection of added sugars in honey, as well as for revealing the fact of feeding bees with various syrups. The authenticity of the botanical and geographical point of honey origin is usually determined by the principle of “fingerprints”. The principle involves collecting the values of an array of indicators and processing them by means of statistical analysis methods. To form a database, in addition to the above methods, methods of NMR spectroscopy, IR spectroscopy, PCR, ICP-MS and some others have become widely used. When determining the authenticity of the botanical and geographical origin of honey, it is also necessary to consider the specific features of local melliferous plants, bee species, soil composition and climatic conditions. Thus, an individual yet integrated approach to the identification of honey by means of the instrumental methods of analysis and statistical processing of results will become a powerful and reliable tool in determining its authenticity, including its botanical and geographical origin.
蜂蜜是一种珍贵的高营养天然产品;它在人口中被广泛消费。天然蜂蜜由于其昂贵的价格,经常成为掺假的对象。蜂蜜的真实性是最重要的质量标准,一方面保证了蜂蜜的生物安全,另一方面提供了健康的市场竞争。在这方面,蜂蜜鉴定问题在所有养蜂文化发达的国家都是相当尖锐的。作者概述了国内外关于蜂蜜真实性的监管文件,以及蜂蜜作为具有受控原产地名称的产品的地位。在对科学文献进行分析的基础上,选择并提出了针对蜂蜜真实性鉴定方法的最有意义的研究。这些研究是在欧洲联盟、中国、巴西、美国、墨西哥和其他国家进行的。这些研究表明,色谱法和同位素质谱法是检测蜂蜜中添加糖最有效的方法,也可以揭示蜜蜂用各种糖浆喂养的事实。蜂蜜产地的植物和地理点的真伪通常是通过“指纹”的原理来确定的。该原则涉及收集一系列指标的值,并通过统计分析方法对其进行处理。为了形成数据库,除上述方法外,还广泛使用了核磁共振光谱、红外光谱、PCR、ICP-MS等方法。在确定蜂蜜的植物和地理来源的真实性时,还需要考虑当地蜜科植物的具体特征、蜜蜂种类、土壤成分和气候条件。因此,通过仪器分析和统计处理结果的方法来鉴定蜂蜜的单独但综合的方法将成为确定其真实性的有力和可靠的工具,包括其植物和地理来源。
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引用次数: 0
New technologies of ice roads construction to maintain the sustainable cold chain of supply in the northern regions of Russia 冰路建设新技术,以维持俄罗斯北部地区可持续的冷链供应
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-245-254
G. Goncharova, S. Borzov, G. V. Borschev
The article reviews the current state of winter ice roads (winter glacial roads) and presents data that confirm the necessity of their service life extension in order to maintain the uninterrupted cold chain of supply in the northern regions of Russia. The regulatory documents that regulate the design engineering and construction of winter ice roads are analyzed. The existing methods and technologies of winter roads construction have been analyzed. The possibility of using the geosynthetic materials for ice roads reinforcement is considered, as well as possible challenges arising from their use are exposed for consideration. The use of chemical modifiers for the creation of structural materials based on water ice is shown to be prospective and promising, especially in combination with the reinforcement of ice blocks. Modern approaches to choice of ice modifying compounds and materials for ice reinforcing are described, as well as criteria achieved on the basis of their application. The scientific studies confirming the possibility of a significant increase in the bearing capacity of glacial coverage and, as a result, an earlier start of their operation is carried out. It is shown that the combined use of reinforcement and ice modifiers allowed the ice sample to maintain its integrity even under exposure to significant deformation loads. The technical possibility of additional reinforcement of ice transport routes as a result of optimizing the modal parameters of their formation, depending on weather conditions, is also described.
本文回顾了冬季冰路(冬季冰川路)的现状,并提出了数据,证实了延长其使用寿命的必要性,以保持俄罗斯北部地区不间断的冷链供应。对规范冬季冰路设计、工程和施工的规范性文件进行了分析。分析了现有的冬季道路施工方法和技术。考虑了使用土工合成材料加固冰路的可能性,以及使用它们可能带来的挑战,以供考虑。使用化学改性剂制造基于水冰的结构材料被证明是有前景的和有前途的,特别是与冰块的加固相结合。介绍了选择冰改性化合物和冰加固材料的现代方法,以及在其应用基础上达到的标准。科学研究证实了冰川覆盖的承载能力有可能显著增加,因此,研究工作已较早开始。结果表明,强化剂和冰改性剂的联合使用使冰样品即使在暴露于显著变形载荷下也能保持其完整性。还描述了根据天气条件优化其形成的模态参数,从而在技术上进一步加强冰运输路线的可能性。
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引用次数: 0
Functional properties of the dietary fibers and their using in the manufacturing technology of fish products 膳食纤维的功能特性及其在鱼制品生产工艺中的应用
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-233-244
T. Pivnenko
This review presents the up-to-date scientific ideas about the role of dietary fibers of plant origin (fiber and hydrocolloids) and animal origin (chitosan and collagen) in the food systems. The main attention is focused on their significance and methods of their using in the manufacturing technology of fish products. Dietary fibers are considered as important components of a diet; the dietary fibers prevent certain disorders and regulate the physical and mental health of the population. Consuming of fish products contributes both to the enrichment of raw materials with functional ingredients, as well as to the increasing in the efficiency of processing of raw materials, including underestimated and underutilized aquatic biological resources. The review shows the influence of dietary fibers on the restructuring of fish muscles systems, their ability to hold water and fats, to stabilize emulsions, to change their sensory characteristics, to increase the yield of the finished food products, and also their ability to influence the shelf life of the finished food product. The mechanisms of involving the certain groups of dietary fibers into the physicochemical and biochemical processes of interaction with the components of fish raw materials are considered; the influence of various conditions of processing on gelation and emulsification is studied. The factors that provide antimicrobial and antioxidant effects of various dietary fibers are given. The properties and examples of using the commercial fiber preparations obtained from various sources are analyzed separately, and the significance of the molecular structure of chitosan and collagen for ensuring the specified technological parameters is considered. It is concluded that using the dietary fiber in the manufacturing process of fish products increases the economic efficiency of production and contributes to the development of the healthy food industry.
本文综述了植物源膳食纤维(纤维和水胶体)和动物源膳食纤维(壳聚糖和胶原蛋白)在食品系统中作用的最新科学观点。重点介绍了它们在鱼类产品生产技术中的意义和应用方法。膳食纤维被认为是饮食的重要组成部分;膳食纤维可以预防某些疾病,调节人群的身心健康。食用鱼产品既有助于原料中功能性成分的丰富,也有助于提高原料加工的效率,包括低估和未充分利用的水生生物资源。这篇综述显示了膳食纤维对鱼类肌肉系统重组的影响,它们保持水和脂肪的能力,稳定乳剂,改变它们的感官特性,增加成品食品的产量,以及它们影响成品食品保质期的能力。探讨了某些膳食纤维与鱼类原料组分相互作用的理化生化过程的机制;研究了不同工艺条件对凝胶和乳化的影响。给出了各种膳食纤维抗菌和抗氧化作用的影响因素。分别分析了各种来源的商品纤维制剂的性能和使用实例,并考虑了壳聚糖和胶原蛋白的分子结构对保证规定工艺参数的意义。综上所述,在鱼制品生产过程中使用膳食纤维可提高生产的经济效益,有利于健康食品产业的发展。
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引用次数: 0
Identification of UFGT-gene-associated groups of Vitis vinifera L. by the developed method of PCR-RFLP genotyping of grape 葡萄PCR-RFLP基因分型方法鉴定葡萄ufgt基因相关群
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-180-187
R. Vafin, I. Mikhailova, I. I. Ageykina
The Vitis vinifera L. UFGT gene is one of the diagnostically significant genes for genetic testing of technical grape varieties as well as wine materials and wines produced from them. The strategy for genetic identification of grape varieties and DNA authentication of wine materials that was previously developed by us and is based on direct sequencing of the specific PCR product with a length of 99 bp gave an impulse to prognostic assessment of feasibility of PCR-RFLP analysis for detection of five diagnostically significant polymorphic positions and the following identification of 13 UFGT gene-associated groups of Vitis vinifera L. The aim of this work consisted in identification of UFGT gene-associated groups of Vitis vinifera L. by detection of diagnostically significant polymorphic positions using the developed PCR-RFLP method for genotyping of grape. Objects of research were 24 samples of technical grape varieties. Their sample preparation was carried out by extracting 50–100 mg of mature grape pulp or stone with its mechanical comminution in a mortar and transfer to an Eppendorf-type tube. Then, nucleic acids were extracted using a commercial innuPREP Plant DNA Kit or DiamondDNA Plant kit. PCR-RFLP with the extracted grape DNA was performed with Phire Plant Direct PCR Master Mix and four selected restrictases (PstI, BsaXI, BtsIMutI and HinfI) according to the protocols presented in the paper. The detection of the PCR-RFLP fragments was performed by visualization of electropherograms in a UV transilluminator after horizontal electrophoresis in 2.5% agarose gel with stained TAE buffer. The method for PCR-RFLP genotyping of grapes developed specially for identification of UFGT gene-associated groups of Vitis vinifera L. by detecting diagnostically significant polymorphic positions demonstrated its feasibility when testing 24 samples of technical grape varieties. With that, the positive result was achieved due to the practical ability of each of four selected restrictases to discriminate the strictly specified polymorphic position generating characteristic PCR-RFLP profiles of 13 UFGT gene-associated groups of Vitis vinifera L., seven of which were revealed during this study. Therefore, as a result of the performed study, the genotypic affiliation of several tested grape varieties was established: six samples were identified as representatives of the UFGT gene-associated group No.1; one sample was assigned to gene-associated group No.2; two samples were characterized by the trait of associated group No.3; four samples belonged to group No. 4; one sample to group No. 5; six samples to group No.13.
Vitis vinifera L. UFGT基因是技术葡萄品种、葡萄酒原料及其生产的葡萄酒进行基因检测的重要诊断基因之一。基因识别战略葡萄酒的葡萄品种和DNA验证材料,以前由美国和基于特定PCR直接测序产品99个基点的长度给一个脉冲的预后评估的可行性PCR-RFLP分析检测5诊断重要的多态位置和以下13 UFGT鉴定相关的基因群葡萄l .这项工作的目的在于利用PCR-RFLP方法对葡萄进行基因分型,检测诊断意义显著的多态性位点,鉴定葡萄UFGT基因相关群。研究对象为24个技术葡萄品种。他们的样品制备是通过在砂浆中提取50-100毫克成熟的葡萄果肉或葡萄石,并将其机械粉碎,然后转移到埃彭多夫型管中进行的。然后,使用商业innuPREP植物DNA试剂盒或DiamondDNA植物试剂盒提取核酸。用Phire Plant Direct PCR Master Mix和四种限制性酶(PstI、BsaXI、BtsIMutI和HinfI)按照本文提出的方案对提取的葡萄DNA进行PCR- rflp。PCR-RFLP片段在2.5%琼脂糖凝胶和染色TAE缓冲液中水平电泳后,在紫外透光仪上显示电泳图。通过对24个技术葡萄品种样品的检测,证明了该方法的可行性。该方法是专门为鉴定葡萄UFGT基因相关群而开发的,检测具有诊断意义的多态性位点。因此,这一阳性结果是由于所选的四种限制性酶都具有实际能力,能够区分严格指定的多态性位置,从而产生13个葡萄UFGT基因相关群体的特征PCR-RFLP图谱,其中7个在本研究中被揭示。因此,通过本研究,确定了几个葡萄品种的基因型隶属关系:6个样品被确定为UFGT基因相关组1的代表;1份样本分为基因相关2组;2个样品具有关联组3的性状;4个样本属于第4组;第5组1个样本;6个样品到第13组。
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引用次数: 0
Study of extraction parameters, quantitative yield of polysaccharides and antioxidant activity of psychrophilic microalgae and cyanobacteria 研究了嗜冷微藻和蓝藻多糖的提取工艺、定量得率及抗氧化活性
IF 2.6 3区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-13 DOI: 10.21323/2618-9771-2023-6-2-202-210
S. Sukhikh, V. Dolganyuk, O. E. Kremleva, E. Ulrikh, E. Kashirskikh, O. Babich
Exopolysaccharides and endopolysaccharides are the main components in the antioxidant complex of psychrophilic microalgae and cyanobacteria. The extraction of these compounds from the cells is really energy consuming, as well as it requires large doses of chemicals due to the resilience, recalcitrance, complexity and diversity of the cell wall in microalgae. The purpose of this article was to study the dependence of polysaccharides quantitative yield on the power of ultrasound treatment and duration of their extraction, as well as to determine the antioxidant activity of the antioxidant complex of psychrophilic microalgae and cyanobacteria. In order to find and confirm the antioxidant properties of the complexes obtained from the microscopic algae biomass, we used the method based on measuring the optical density (in a liquid nutrient medium), i. e. the method for determining the antioxidant activity of the samples under research by their ability to reduce the level of free radicals. As a result of the studies the rational conditions were found for the extraction of the antioxidant complex from the cell culture fluid, and from the cell-related psychrophilic microalgae and cyanobacteria Skeletonema pseudocostatum, Thalassiosira pseudonana, Fragilariopsis kerguelensis, Aphanizomenon gracile, and Anabaena cylindrica. For the exopolysaccharides extraction from the psychrophilic microalgae and cyanobacteria Skeletonema pseudocostatum, Thalassiosira pseudonana, Fragilariopsis kerguelensis, Aphanizomenon gracile and Anabaena cylindrica, the method of ethanol extraction with an extraction module of 1:2 and an extraction temperature of 5 °С was used. The ability of psychrophilic microalgae and cyanobacteria Skeletonema pseudocostatum, Thalassiosira pseudonana, Fragilariopsis kerguelensis, Aphanizomenon gracile and Anabaena cylindrica to produce an antioxidant complex was studied. It was found that this complex contains polysaccharides: endopolysaccharides and exopolysaccharides in particular. The ability of psychrophilic microalgae and cyanobacteria Skeletonema pseudocostatum, Thalassiosira pseudonana, Fragilariopsis kerguelensis, Aphanizomenon gracile, and Anabaena cylindrica to produce an antioxidant complex was proven by the presence of significant antioxidant activity of psychrophilic microalgae and cyanobacteria, determined and confirmed by the methods ABTS, DPPH, and FRAP. The psychrophilic microalga Skeletonema pseudocostatum possesses the highest antioxidant activity. The availability of antioxidant properties in psychrophilic microalgae and cyanobacteria opens up the prospects for their practical application.
胞外多糖和胞内多糖是嗜冷微藻和蓝藻抗氧化复合物的主要成分。从细胞中提取这些化合物是非常消耗能量的,而且由于微藻细胞壁的弹性、顽固性、复杂性和多样性,它需要大剂量的化学物质。研究了超声处理功率和提取时间对多糖定量产率的影响,并测定了亲冷微藻和蓝藻的抗氧化复合物的抗氧化活性。为了发现和确认从微观藻类生物量中获得的配合物的抗氧化性能,我们采用了基于测量光密度的方法(在液体营养介质中),即通过研究样品降低自由基水平的能力来确定其抗氧化活性的方法。通过研究,找到了从细胞培养液中提取抗氧化复合物的合理条件,以及从与细胞相关的嗜冷微藻和蓝藻中提取抗氧化复合物的条件,这些蓝藻包括假骨藻、假海藻、kerguelfragilariopsis、Aphanizomenon gracile和水螅。对嗜冷微藻和蓝藻pseudocostatum、Thalassiosira pseudonana、Fragilariopsis kerguelensis、Aphanizomenon gracile和Anabaena ica的胞外多糖的提取,采用乙醇提取法,提取模比为1:2,提取温度为5°С。研究了嗜冷微藻和蓝藻:假腹骨藻、假海藻、克格尔碎藻、瘦弱单胞菌和白茅水藻产生抗氧化复合物的能力。发现该复合物含有多糖,特别是内多糖和外多糖。通过ABTS、DPPH和FRAP方法测定并证实了嗜冷微藻和蓝藻的显著抗氧化活性,证明了嗜冷微藻和蓝藻产生抗氧化复合物的能力。嗜冷微藻假肋骨藻具有最高的抗氧化活性。在嗜冷微藻和蓝藻中抗氧化性能的可用性为其实际应用开辟了前景。
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Agroecology and Sustainable Food Systems
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