Background: Mucous membrane pemphigoid (MMP) is an autoimmune disease characterized by scarring lesions at mucosal sites. Although the pathogenic role of specific IgG and/or IgA has been already demonstrated and the detection of these immunoglobulins is a criterion in the diagnosis of MMP, little is known about IgE role in this disease. Therefore, the main purpose of this study was to assess the presence of circulating and tissue bound IgE in patients with MMP and their possible correlations with clinical presentation and disease course.
Methods: We conducted a retrospective study on 29 patients affected by MMP, recruited from a single center. Direct and indirect immunofluorescence studies were assessed to analyze the presence of specific IgE directed against the basal membrane zone. For each patient, fluorescence data were compared to clinical features.
Results: Linear deposits of C3, IgG and IgA were present in 86.2%, 62% and 37.9% of cases respectively, while IgE linear deposits were detected in 17 out of 29 patients (58.6%) including one case with isolated IgE positivity. Circulating IgE and IgA anti-BMZ were present in 7 (24.1%) and 5 (17.2%) patients, respectively. Both the presence of circulating IgA and of tissue bound IgE deposits correlated with disease activity index (P<0.014).
Conclusions: Our results demonstrated the presence of IgE autoantibodies in MMP, particularly in more severe cases. Thus, IgE detection may represent an additional useful diagnostic tool in this disease.
{"title":"Detection of IgE autoantibodies in mucous membrane pemphigoid and their association with disease severity.","authors":"Laura Corti, Daniele Fanoni, Luigia Venegoni, Simona Muratori, Sebastiano Recalcati, Emilio Berti","doi":"10.23736/S0392-0488.18.06167-9","DOIUrl":"https://doi.org/10.23736/S0392-0488.18.06167-9","url":null,"abstract":"<p><strong>Background: </strong>Mucous membrane pemphigoid (MMP) is an autoimmune disease characterized by scarring lesions at mucosal sites. Although the pathogenic role of specific IgG and/or IgA has been already demonstrated and the detection of these immunoglobulins is a criterion in the diagnosis of MMP, little is known about IgE role in this disease. Therefore, the main purpose of this study was to assess the presence of circulating and tissue bound IgE in patients with MMP and their possible correlations with clinical presentation and disease course.</p><p><strong>Methods: </strong>We conducted a retrospective study on 29 patients affected by MMP, recruited from a single center. Direct and indirect immunofluorescence studies were assessed to analyze the presence of specific IgE directed against the basal membrane zone. For each patient, fluorescence data were compared to clinical features.</p><p><strong>Results: </strong>Linear deposits of C3, IgG and IgA were present in 86.2%, 62% and 37.9% of cases respectively, while IgE linear deposits were detected in 17 out of 29 patients (58.6%) including one case with isolated IgE positivity. Circulating IgE and IgA anti-BMZ were present in 7 (24.1%) and 5 (17.2%) patients, respectively. Both the presence of circulating IgA and of tissue bound IgE deposits correlated with disease activity index (P<0.014).</p><p><strong>Conclusions: </strong>Our results demonstrated the presence of IgE autoantibodies in MMP, particularly in more severe cases. Thus, IgE detection may represent an additional useful diagnostic tool in this disease.</p>","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36557394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01Epub Date: 2018-10-22DOI: 10.23736/S0392-0488.18.06180-1
Filomena Russo, Martina Vispi, Silvia Bocci, Virginia Mancini, Riccardo Sirna, Fabio Giannini, Michele Pellegrino
{"title":"New onset psoriasis in a patient with chronic inflammatory demyelinating polyneuropathy treated with Rituximab.","authors":"Filomena Russo, Martina Vispi, Silvia Bocci, Virginia Mancini, Riccardo Sirna, Fabio Giannini, Michele Pellegrino","doi":"10.23736/S0392-0488.18.06180-1","DOIUrl":"https://doi.org/10.23736/S0392-0488.18.06180-1","url":null,"abstract":"","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36598527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01Epub Date: 2018-11-12DOI: 10.23736/S0392-0488.18.06149-7
Marta Carlesimo, Enrico Cortesi, Andrea D'Arino, Flavia Pigliacelli, Gemma Caro, Serena Zancla, Caterina M Fortuna, Federico Grifalchi, Alfredo Rossi
{"title":"Isochemotopic response: the first case on a scar.","authors":"Marta Carlesimo, Enrico Cortesi, Andrea D'Arino, Flavia Pigliacelli, Gemma Caro, Serena Zancla, Caterina M Fortuna, Federico Grifalchi, Alfredo Rossi","doi":"10.23736/S0392-0488.18.06149-7","DOIUrl":"https://doi.org/10.23736/S0392-0488.18.06149-7","url":null,"abstract":"","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36726014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01Epub Date: 2019-05-14DOI: 10.23736/S0392-0488.19.06181-9
Hilmi Atay, Engin Kelkitli, Mehmet Turgut
{"title":"A rare side effect: eltrombopag associated hyperpigmentation.","authors":"Hilmi Atay, Engin Kelkitli, Mehmet Turgut","doi":"10.23736/S0392-0488.19.06181-9","DOIUrl":"https://doi.org/10.23736/S0392-0488.19.06181-9","url":null,"abstract":"","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37256896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01Epub Date: 2020-04-29DOI: 10.23736/S0392-0488.20.06551-7
Eleonora Farinazzo, Roberta Giuffrida, Caterina Dianzani, Roberta Vezzoni, Chiara Retrosi, Iris Zalaudek, Claudio Conforti
Inflammatory response in the skin is important for host defense against a broad spectrum of insults including microorganisms, chemicals, radiation and physical trauma. When the inflammatory process becomes prolonged and self-perpetuating, illness occurs. In this review, we discuss recent findings regarding the complex inflammatory mechanisms in allergic contact dermatitis, atopic dermatitis and psoriasis.
{"title":"What makes an inflammatory disease inflammatory? An overview of inflammatory mechanisms of allergic contact dermatitis, atopic dermatitis and psoriasis.","authors":"Eleonora Farinazzo, Roberta Giuffrida, Caterina Dianzani, Roberta Vezzoni, Chiara Retrosi, Iris Zalaudek, Claudio Conforti","doi":"10.23736/S0392-0488.20.06551-7","DOIUrl":"https://doi.org/10.23736/S0392-0488.20.06551-7","url":null,"abstract":"<p><p>Inflammatory response in the skin is important for host defense against a broad spectrum of insults including microorganisms, chemicals, radiation and physical trauma. When the inflammatory process becomes prolonged and self-perpetuating, illness occurs. In this review, we discuss recent findings regarding the complex inflammatory mechanisms in allergic contact dermatitis, atopic dermatitis and psoriasis.</p>","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37883175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01Epub Date: 2018-11-09DOI: 10.23736/S0392-0488.18.06120-5
Vincenzo Greco, Matteo Megna, Maria A Luciano, Gabriella Fabbrocini
{"title":"Pityriasis versicolor with uncommon localizations.","authors":"Vincenzo Greco, Matteo Megna, Maria A Luciano, Gabriella Fabbrocini","doi":"10.23736/S0392-0488.18.06120-5","DOIUrl":"https://doi.org/10.23736/S0392-0488.18.06120-5","url":null,"abstract":"","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36665416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of nine patients with solar urticaria during summer.","authors":"Nicola Milanesi, Massimo Gola, Stefano Francalanci","doi":"10.23736/S0392-0488.19.06176-5","DOIUrl":"https://doi.org/10.23736/S0392-0488.19.06176-5","url":null,"abstract":"","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37101284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meeting a rare disease: bullous ichthyosiform erythroderma.","authors":"Giulia Tonini, Emanuele Trovato, Michele Pellegrino, Michele Fimiani","doi":"10.23736/S0392-0488.18.06058-3","DOIUrl":"https://doi.org/10.23736/S0392-0488.18.06058-3","url":null,"abstract":"","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36307036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.23736/S0392-0488.20.06825-X
Ling Yang, Zhonghua Hu, Yanxia Jin, Ning Huang, Su-Ping Xu
BACKGROUND To investigate the role and underlying mechanism of miR-4497 in oxidative stress and inflammatory injury in keratinocytes induced by ultraviolet B (UVB) radiation. METHODS An injury model of keratinocytes induced by UVB radiation was constructed. RT-qPCR, MTT assay and flow cytometry were adopted to detect miR-4497 expression in HaCaT cells, cell proliferation, and cell apoptosis, respectively. The levels of cytokines TNF-α, IL-18, IL-6 and IL-1β in cell culture supernatant were tested by ELISA. ROS levels in the cells were labeled by DCFH-DA fluorescent probe, and then quantitative fluorescence analysis was performed by flow cytometry. SOD activity in the cells was measured by Xanthine Oxidase assay kit. Western blot was used to determine NF-κB expression in cytoplasm and nucleus, and p-IκBα expression in the cells. RESULTS UVB radiation significantly increased miR-4497 expression in HaCaT cells, inhibited cell proliferation, and promoted cell apoptosis. Meanwhile, UVB radiation caused the promotion of secretion of cytokines TNF-α, IL-18, IL-6 and IL-1β. The production of reactive oxygen species (ROS) was promoted by UVB radiation, while SOD activity was inhibited. Nuclear transfer of NF-κB signal was also induced by UVB radiation. In addition, downregulation of miR-4497 expression significantly inhibited the effects of UVB radiation on cell proliferation, apoptosis, cytokine secretion, redox level and NF-κB signal in HaCaT cells, while overexpression of miR-4497 further enhanced these effects of UVB radiation on HaCaT cells. CONCLUSIONS UVB may promote the expression of inflammatory and oxidative stress signals in keratinocytes by upregulating miR-4497 expression, thus mediating cell injury.
{"title":"MiR-4497 mediates oxidative stress and inflammatory injury in keratinocytes induced by ultraviolet B radiation through regulating NF-κB expression.","authors":"Ling Yang, Zhonghua Hu, Yanxia Jin, Ning Huang, Su-Ping Xu","doi":"10.23736/S0392-0488.20.06825-X","DOIUrl":"https://doi.org/10.23736/S0392-0488.20.06825-X","url":null,"abstract":"BACKGROUND\u0000To investigate the role and underlying mechanism of miR-4497 in oxidative stress and inflammatory injury in keratinocytes induced by ultraviolet B (UVB) radiation.\u0000\u0000\u0000METHODS\u0000An injury model of keratinocytes induced by UVB radiation was constructed. RT-qPCR, MTT assay and flow cytometry were adopted to detect miR-4497 expression in HaCaT cells, cell proliferation, and cell apoptosis, respectively. The levels of cytokines TNF-α, IL-18, IL-6 and IL-1β in cell culture supernatant were tested by ELISA. ROS levels in the cells were labeled by DCFH-DA fluorescent probe, and then quantitative fluorescence analysis was performed by flow cytometry. SOD activity in the cells was measured by Xanthine Oxidase assay kit. Western blot was used to determine NF-κB expression in cytoplasm and nucleus, and p-IκBα expression in the cells.\u0000\u0000\u0000RESULTS\u0000UVB radiation significantly increased miR-4497 expression in HaCaT cells, inhibited cell proliferation, and promoted cell apoptosis. Meanwhile, UVB radiation caused the promotion of secretion of cytokines TNF-α, IL-18, IL-6 and IL-1β. The production of reactive oxygen species (ROS) was promoted by UVB radiation, while SOD activity was inhibited. Nuclear transfer of NF-κB signal was also induced by UVB radiation. In addition, downregulation of miR-4497 expression significantly inhibited the effects of UVB radiation on cell proliferation, apoptosis, cytokine secretion, redox level and NF-κB signal in HaCaT cells, while overexpression of miR-4497 further enhanced these effects of UVB radiation on HaCaT cells.\u0000\u0000\u0000CONCLUSIONS\u0000UVB may promote the expression of inflammatory and oxidative stress signals in keratinocytes by upregulating miR-4497 expression, thus mediating cell injury.","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78728153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-23DOI: 10.23736/S0392-0488.20.06673-0
A. Raimondo, F. Salzano, S. Lembo
{"title":"Disseminated mucocutaneous Candida infection during anti IL-17A therapy in a psoriatic patient.","authors":"A. Raimondo, F. Salzano, S. Lembo","doi":"10.23736/S0392-0488.20.06673-0","DOIUrl":"https://doi.org/10.23736/S0392-0488.20.06673-0","url":null,"abstract":"","PeriodicalId":49071,"journal":{"name":"Giornale Italiano Di Dermatologia E Venereologia","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2020-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82495548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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