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The Tumor Necrosis Factor-α Level in Platelet-Rich Plasma Might Be Associated with Treatment Outcome in Patients with Interstitial Cystitis/Bladder Pain Syndrome or Recurrent Urinary Tract Infection 富血小板血浆中的肿瘤坏死因子-α水平可能与间质性膀胱炎/膀胱疼痛综合征或复发性尿路感染患者的治疗效果有关
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010163
J. Jhang, Yuan-Hong Jiang, Teng-Yi Lin, H. Kuo
Using platelet-rich plasma (PRP) injections to treat urological diseases has attracted great attention. This study investigated the impact of cytokine concentrations in PRP on the treatment outcome of patients with recurrent urinary tract infection (rUTI) and interstitial cystitis/bladder pain syndrome (IC/BPS). Forty patients with IC/BPS and twenty-one patients with rUTI were enrolled for four-monthly repeated PRP injections. PRP was collected at the first injection and analyzed with multiplex immunoassays for 12 target cytokines. In patients with IC/BPS, a Global Response Assessment (GRA) score ≥ 2 was defined as a successful outcome. In rUTI patients, ≤2 episodes of UTI recurrence during one year of follow-up was considered a successful outcome. Nineteen (47.5%) patients with IC/BPS and eleven (52.4%) patients with rUTI had successful outcomes. The IC/BPS patients with successful outcomes had significantly lower levels of tumor necrosis factor-α (TNF-α) in their PRP than those with unsuccessful outcomes (p = 0.041). The rUTI patients with successful outcomes also had a lower level of TNF-α (p = 0.025) and a higher level of epidermal growth factor (p = 0.035) and transforming growth factor-β2 (p = 0.024) in PRP than those with unsuccessful outcomes. A lower level of TNF-α in PRP might be a potentially predictive factor of treatment outcome.
利用富血小板血浆(PRP)注射治疗泌尿系统疾病已引起广泛关注。本研究调查了血小板富集血浆中细胞因子浓度对复发性尿路感染(rUTI)和间质性膀胱炎/膀胱疼痛综合征(IC/BPS)患者治疗效果的影响。40名IC/BPS患者和21名复发性尿路感染患者参加了为期四个月的PRP重复注射。在第一次注射时收集 PRP,并用多重免疫测定法分析 12 种目标细胞因子。在 IC/BPS 患者中,全球反应评估 (GRA) 评分≥ 2 分即为成功。在 rUTI 患者中,随访一年期间UTI 复发次数≤2 次即为成功。19名(47.5%)IC/BPS 患者和 11 名(52.4%)rUTI 患者获得了成功结果。成功治愈的 IC/BPS 患者 PRP 中肿瘤坏死因子-α(TNF-α)的水平明显低于失败治愈的患者(p = 0.041)。与治疗失败的患者相比,治疗成功的 rUTI 患者的 PRP 中 TNF-α 水平较低(p = 0.025),表皮生长因子(p = 0.035)和转化生长因子-β2(p = 0.024)水平较高。PRP中较低水平的TNF-α可能是治疗结果的潜在预测因素。
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引用次数: 0
PSEN1 His214Asn Mutation in a Korean Patient with Familial EOAD and the Importance of Histidine–Tryptophan Interactions in TM-4 Stability 一名韩国家族性 EOAD 患者的 PSEN1 His214Asn 突变与组氨酸-色氨酸相互作用在 TM-4 稳定性中的重要性
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010116
Eva Bagyinszky, Minju Kim, Young Ho Park, S. S. A. An, Sangyun Kim
A pathogenic mutation in presenilin-1 (PSEN1), His214Asn, was found in a male patient with memory decline at the age of 41 in Korea for the first time. The proband patient was associated with a positive family history from his father, paternal aunt, and paternal grandmother without genetic testing. He was diagnosed with early onset Alzheimer’s disease (EOAD). PSEN1 His214Asn was initially reported in an Italian family, where the patient developed phenotypes similar to the current proband patient. Magnetic resonance imaging (MRI) scans revealed a mild hippocampal atrophy. The amyloid positron emission tomography (amyloid-PET) was positive, along with the positive test results of the increased amyloid ß (Aβ) oligomerization tendency with blood. The PSEN1 His214 amino acid position plays a significant role in the gamma–secretase function, especially from three additional reported mutations in this residue: His214Asp, His214Tyr, and His214Arg. The structure prediction model revealed that PSEN1 protein His214 may interact with Trp215 of His-Trp cation-π interaction, and the mutations of His214 would destroy this interaction. The His-Trp cation-π interaction between His214 and Trp215 would play a crucial structural role in stabilizing the 4th transmembrane domain of PSEN1 protein, especially when aromatic residues were often reported in the membrane interface of the lipid–extracellular region of alpha helices or beta sheets. The His214Asn would alter the cleavage dynamics of gamma–secretase from the disappeared interactions between His214 and Trp215 inside of the helix, resulting in elevated amyloid production. Hence, the increased Aβ was reflected in the increased Aβ oligomerization tendency and the accumulations of Aβ in the brain from amyloid-PET, leading to EOAD.
韩国首次在一名 41 岁记忆力衰退的男性患者身上发现了 presenilin-1(PSEN1)的致病突变 His214Asn。该患者的父亲、姑姑和祖母均有阳性家族史,但未进行基因检测。他被诊断为早发性阿尔茨海默病(EOAD)。PSEN1 His214Asn最初是在一个意大利家庭中被报道的,该家庭中的患者出现了与目前的疑似患者相似的表型。磁共振成像(MRI)扫描显示海马轻度萎缩。淀粉样蛋白正电子发射断层扫描(amyloid-PET)呈阳性,同时血液中淀粉样蛋白ß(Aβ)寡聚化倾向也呈阳性。PSEN1的His214氨基酸位置在γ-分泌酶的功能中起着重要作用,特别是在该残基上又有三个突变报道:His214Asp、His214Tyr和His214Arg。结构预测模型显示,PSEN1 蛋白中的 His214 可能与 His-Trp 阳离子-π相互作用的 Trp215 发生相互作用,而 His214 的突变将破坏这种相互作用。His214 与 Trp215 之间的 His-Trp 阳离子-π 相互作用在稳定 PSEN1 蛋白的第 4 跨膜结构域方面起着至关重要的结构作用,尤其是当芳香族残基经常出现在α螺旋或β片的脂质-胞外区域的膜界面时。His214Asn 会改变γ-分泌酶在螺旋内 His214 和 Trp215 相互作用消失后的裂解动力学,从而导致淀粉样蛋白生成量增加。因此,Aβ的增加反映在淀粉样蛋白-PET增加的Aβ寡聚化趋势和Aβ在大脑中的积累,从而导致EOAD。
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引用次数: 0
Expanding the Phenotype of Hereditary Congenital Facial Paresis Type 3 扩展遗传性先天性面瘫 3 型的表型
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010129
Aysylu Murtazina, A. Borovikov, A. Kuchina, Olga Ovsova, M. Bulakh, A. Chukhrova, Svetlana Braslavskaya, O. Ryzhkova, Nikolay A. Skryabin, S. Kutsev, E. Dadali
The HOXB1 gene encodes a homeobox transcription factor pivotal in the development of rhombomere 4. Biallelic pathogenic variants in this gene are associated with congenital facial paresis type 3 (HCFP3). Only seven single nucleotide variants have been reported in the literature to date. Here, we report a 27-year-old female with a unique presentation of HCFP3 with two novel compound-heterozygous missense variants: c.763C>G, p.(Arg255Gly), which arose de novo and an inherited c.781C>T, p.(Arg261Cys) variant. The patient exhibited HCFP3 symptoms with mild upward esodeviation and lacked the documented ear malformations common in HCFP. For many years, she was misdiagnosed with facio-scapulo-humeral muscular dystrophy, due to complaints of shoulder girdle and neck muscle weakness. No alternative genetic or acquired causes of neck and shoulder girdle weakness were found, suggesting its potential inclusion in the phenotypic spectrum.
HOXB1 基因编码一种同源转录因子,在菱形体 4 的发育过程中起着关键作用。该基因的双叶致病变异与先天性面瘫 3 型(HCFP3)有关。迄今为止,文献中仅报道了 7 个单核苷酸变异。在此,我们报告了一名 27 岁女性的独特 HCFP3 病例,她患有两个新的复合杂合子错义变异:c.763C>G,p.(Arg255Gly),这是一个从头开始出现的变异,以及一个遗传性 c.781C>T,p.(Arg261Cys)变异。患者表现出 HCFP3 症状,并伴有轻度上腭外翻,但没有 HCFP 常见的耳畸形记录。多年来,她一直被误诊为面-肩-肱肌营养不良症,因为她主诉肩腰和颈部肌肉无力。没有发现导致颈肩肌无力的其他遗传或获得性原因,这表明该病可能属于表型谱系。
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引用次数: 0
Post-Radiotherapy Exosomal Non-Coding RNA and Hemograms for Early Death Prediction in Patients with Cervical Cancer 预测宫颈癌患者放疗后早期死亡的外泌体非编码 RNA 和血象图
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010126
O. Cho
Concurrent chemo-radiotherapy (CCRT) is linked with accelerated disease progression and early death (ED) in various cancers. This study aimed to assess the association of plasma levels of exosomal non-coding ribonucleic acid (RNA) (ncRNA) and blood cell dynamics with ED prediction in patients with cervical cancer undergoing CCRT. Using propensity score matching, a comparison of complete blood counts (CBCs) was performed among 370 CCRT-treated patients. Differences in ncRNA and messenger RNA (mRNA) expression before and after CCRT in 84 samples from 42 patients (cohort 2) were represented as logarithmic fold change (log2FC). Networks were constructed to link the CBCs to the RNAs whose expression correlated with ED. From the key RNAs selected using multiple regression of all RNA combinations in the network, CBC dynamics-associated ncRNAs were functionally characterized using an enrichment analysis. Cohort 1 (120 patients) exhibited a correlation between elevated absolute neutrophil counts (ANC) and ED. Cohort 2 exhibited a prevalence of microRNA (miR)-574-3p and long intergenic non-protein coding (LINC)01003 ncRNA, whose expression correlated with ANC and hemoglobin values, respectively. Conversely, acyl-coenzyme A thioesterase 9 (ACOT9) mRNA was relevant to all CBC components. An integrative analysis of post-CCRT ncRNA levels and CBC values revealed that the patients with miR-574-3p-LINC01003-ACOT9 log2FC) < 0 had a better prospect of 30-month disease-specific survival. These findings indicate that miR-574-3p and LINC01003 could serve as ED prognostic biomarkers.
同期放化疗(CCRT)与各种癌症的疾病进展加速和早期死亡(ED)有关。本研究旨在评估接受CCRT治疗的宫颈癌患者血浆中外泌体非编码核糖核酸(RNA)(ncRNA)水平和血细胞动态与ED预测的相关性。通过倾向得分匹配,对 370 名接受 CCRT 治疗的患者的全血细胞计数(CBC)进行了比较。42 名患者(队列 2)的 84 份样本在 CCRT 治疗前后的 ncRNA 和信使 RNA (mRNA) 表达差异以对数折叠变化(log2FC)表示。通过构建网络将 CBC 与 ED 表达相关的 RNA 联系起来。通过对网络中的所有 RNA 组合进行多元回归,从中筛选出关键 RNA,并利用富集分析对与 CBC 动态相关的 ncRNA 进行功能表征。队列 1(120 名患者)显示出中性粒细胞绝对计数(ANC)升高与 ED 之间的相关性。队列 2 显示,microRNA (miR)-574-3p 和长基因间非蛋白编码 (LINC)01003 ncRNA 的流行,其表达分别与 ANC 和血红蛋白值相关。相反,酰基辅酶 A 硫代酯酶 9 (ACOT9) mRNA 与所有 CBC 成分相关。对CCRT后ncRNA水平和CBC值的综合分析表明,miR-574-3p-LINC01003-ACOT9 log2FC)< 0的患者30个月的疾病特异性生存率更高。这些发现表明,miR-574-3p 和 LINC01003 可作为 ED 的预后生物标志物。
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引用次数: 0
Comprehensive Evaluation of Polyaniline-Doped Lignosulfonate in Adsorbing Dye and Heavy Metal Ions 聚苯胺掺杂木质素磺酸盐吸附染料和重金属离子的综合评估
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010133
Wenjuan Wu, Penghui Li, Mingkang Wang, Huijun Liu, Xiufu Zhao, Cai-Wen Wu, Jianpeng Ren
Lignosulfonate/polyaniline (LS/PANI) nanocomposite adsorbent materials were prepared by the chemical polymerization of lignosulfonate with an aniline monomer as a dopant and structure-directing agent, and the adsorption behavior of dyes as well as heavy metal ions was investigated. LS/PANI composites were used as dye adsorbents for the removal of different cationic dyes (malachite green, methylene blue, and crystal violet). The adsorption behavior of LS/PANI composites as dye adsorbents for malachite green was investigated by examining the effects of the adsorbent dosage, solution pH, initial concentration of dye, adsorption time, and temperature on the adsorption properties of this dye. The following conclusions were obtained. The optimum adsorption conditions for the removal of malachite green dye when LS/PANI composites were used as malachite green dye adsorbents were as follows: an adsorbent dosage of 20 mg, an initial concentration of the dye of 250 mg/L, an adsorption time of 300 min, and a temperature of 358 K. The LS/PANI composite adsorbed malachite green dye in accordance with the Langmuir adsorption model and pseudo-second-order kinetic model, which belongs to chemisorption-based monomolecular adsorption, and the equilibrium adsorption amount was 245.75 mg/g. In particular, the adsorption of heavy metal ion Pb2+ was investigated, and the removal performance was also favorable for Pb2+.
木质素磺酸盐/聚苯胺(LS/PANI)纳米复合吸附材料由木质素磺酸盐与苯胺单体作为掺杂剂和结构引导剂进行化学聚合制备而成,并研究了其对染料和重金属离子的吸附行为。将 LS/PANI 复合材料用作染料吸附剂,用于去除不同的阳离子染料(孔雀石绿、亚甲基蓝和结晶紫)。通过研究吸附剂用量、溶液 pH 值、染料初始浓度、吸附时间和温度对孔雀石绿吸附性能的影响,考察了 LS/PANI 复合材料作为染料吸附剂对该染料的吸附行为。得出以下结论。将 LS/PANI 复合材料用作孔雀石绿染料吸附剂时,去除孔雀石绿染料的最佳吸附条件如下:吸附剂用量为 20 mg,染料初始浓度为 250 mg/L,吸附时间为 300 min,温度为 358 K。LS/PANI复合材料对孔雀石绿染料的吸附符合Langmuir吸附模型和伪二阶动力学模型,属于化学吸附型单分子吸附,平衡吸附量为245.75 mg/g。特别是对重金属离子 Pb2+ 的吸附进行了研究,对 Pb2+ 的去除性能也很好。
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引用次数: 0
The Role of miRNAs in Childhood Acute Lymphoblastic Leukemia Relapse and the Associated Molecular Mechanisms miRNA 在儿童急性淋巴细胞白血病复发中的作用及相关分子机制
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010119
Dalia Barrios-Palacios, Jorge Organista-Nava, J. Balandrán, L. Alarcón-Romero, Ma Isabel Zubillaga-Guerrero, B. Illades-Aguiar, Alinne Ayulieth Rivas-Alarcón, Jessica Julieth Diaz-Lucas, Yazmín Gómez-Gómez, M. Leyva-Vázquez
Acute lymphoblastic leukemia (ALL) is the most common cancer in children worldwide. Although ALL patients’ overall survival rates in wealthy countries currently surpass 80%, 15–20% of patients still experience relapse. The underlying mechanisms of relapse are still not fully understood, and little progress has been made in treating refractory or relapsed disease. Disease relapse and treatment failure are common causes of leukemia-related death. In ALL relapse, several gene signatures have been identified, but it is also important to study miRNAs involved in ALL relapse in an effort to avoid relapse and to achieve better survival rates since miRNAs regulate target genes that participate in signaling pathways involved in relapse, such as those related to drug resistance, survival signals, and antiapoptotic mechanisms. Several miRNAs, such as miR-24, miR-27a, miR-99/100, miR-124, miR-1225b, miR-128b, miR-142-3p, miR-155 and miR-335-3p, are valuable biomarkers for prognosis and treatment response in ALL patients. Thus, this review aimed to analyze the primary miRNAs involved in pediatric ALL relapse and explore the underlying molecular mechanisms in an effort to identify miRNAs that may be potential candidates for anti-ALL therapy soon.
急性淋巴细胞白血病(ALL)是全球最常见的儿童癌症。虽然目前在富裕国家,ALL 患者的总生存率超过 80%,但仍有 15-20% 的患者会复发。复发的根本机制尚未完全明了,在治疗难治或复发疾病方面进展甚微。疾病复发和治疗失败是白血病相关死亡的常见原因。在ALL复发中,已经发现了一些基因特征,但研究参与ALL复发的miRNA也很重要,因为miRNA可调控参与复发信号通路的靶基因,如与耐药性、生存信号和抗凋亡机制有关的靶基因,从而避免复发,提高生存率。一些miRNA,如miR-24、miR-27a、miR-99/100、miR-124、miR-1225b、miR-128b、miR-142-3p、miR-155和miR-335-3p,是ALL患者预后和治疗反应的重要生物标志物。因此,本综述旨在分析参与小儿ALL复发的主要miRNAs,并探讨其潜在的分子机制,从而找出可能很快成为抗ALL治疗潜在候选者的miRNAs。
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引用次数: 0
Metacyclogenesis as the Starting Point of Chagas Disease Metacyclogenesis 是南美锥虫病的起点
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010117
Alessandro Zanard Lopes Ferreira, C. D. de Araújo, Isabela Cunha Costa Cardoso, Karen Stephanie de Souza Mangabeira, A. Rocha, Sébastien Charneau, Jaime Martins Santana, F. N. Motta, I.M.D. Bastos
Chagas disease is a neglected infectious disease caused by the protozoan Trypanosoma cruzi, primarily transmitted by triatomine vectors, and it threatens approximately seventy-five million people worldwide. This parasite undergoes a complex life cycle, transitioning between hosts and shifting from extracellular to intracellular stages. To ensure its survival in these diverse environments, T. cruzi undergoes extreme morphological and molecular changes. The metacyclic trypomastigote (MT) form, which arises from the metacyclogenesis (MTG) process in the triatomine hindgut, serves as a crucial link between the insect and human hosts and can be considered the starting point of Chagas disease. This review provides an overview of the current knowledge regarding the parasite’s life cycle, molecular pathways, and mechanisms involved in metabolic and morphological adaptations during MTG, enabling the MT to evade the immune system and successfully infect human cells.
南美锥虫病是一种被忽视的传染病,由原生动物南美锥虫引起,主要通过三蠹类媒介传播,威胁着全球约 7 500 万人。这种寄生虫经历了复杂的生命周期,在宿主之间转换,并从细胞外阶段转入细胞内阶段。为了确保在这些不同的环境中生存,T. cruzi经历了极端的形态和分子变化。在三疣梭子蟹后肠中的变态噬菌体(MTG)过程中产生的变态噬菌体(MT)形态是昆虫宿主和人类宿主之间的关键纽带,可被视为南美锥虫病的起点。本综述概述了目前有关寄生虫生命周期、分子途径以及 MTG 过程中代谢和形态适应机制的知识,这些知识使 MT 能够躲避免疫系统并成功感染人类细胞。
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引用次数: 0
Biotic and Abiotic Stressors in Plant Metabolism 植物新陈代谢中的生物和非生物压力源
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010121
L. Cornara, M. Mandrone, A. Smeriglio
Plants are subject to a variety of biotic and abiotic stress that affect their metabolism, physiology, morphology, and growth [...]
植物会受到各种生物和非生物胁迫,从而影响其新陈代谢、生理、形态和生长 [...] 。
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引用次数: 0
Genetic Alterations of NF-κB and Its Regulators: A Rich Platform to Advance Colorectal Cancer Diagnosis and Treatment NF-κB 及其调控因子的基因改变:推进结直肠癌诊断和治疗的丰富平台
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010154
Faranak Alipourgivi, Aishat Motolani, Alice Y. Qiu, Wenan Qiang, Guang-Yu Yang, Shuibing Chen, Tao Lu
Colorectal cancer (CRC) is the third leading cause of cancer mortality in the United States, with an estimated 52,000 deaths in 2023. Though significant progress has been made in both diagnosis and treatment of CRC in recent years, genetic heterogeneity of CRC—the culprit for possible CRC relapse and drug resistance, is still an insurmountable challenge. Thus, developing more effective therapeutics to overcome this challenge in new CRC treatment strategies is imperative. Genetic and epigenetic changes are well recognized to be responsible for the stepwise development of CRC malignancy. In this review, we focus on detailed genetic alteration information about the nuclear factor (NF)-κB signaling, including both NF-κB family members, and their regulators, such as protein arginine methyltransferase 5 (PRMT5), and outer dynein arm docking complex subunit 2 (ODAD2, also named armadillo repeat-containing 4, ARMC4), etc., in CRC patients. Moreover, we provide deep insight into different CRC research models, with a particular focus on patient-derived xenografts (PDX) and organoid models, and their potential applications in CRC research. Genetic alterations on NF-κB signaling components are estimated to be more than 50% of the overall genetic changes identified in CRC patients collected by cBioportal for Cancer Genomics; thus, emphasizing its paramount importance in CRC progression. Consequently, various genetic alterations on NF-κB signaling may hold great promise for novel therapeutic development in CRC. Future endeavors may focus on utilizing CRC models (e.g., PDX or organoids, or isogenic human embryonic stem cell (hESC)-derived colonic cells, or human pluripotent stem cells (hPSC)-derived colonic organoids, etc.) to further uncover the underpinning mechanism of these genetic alterations in NF-κB signaling in CRC progression. Moreover, establishing platforms for drug discovery in dishes, and developing Biobanks, etc., may further pave the way for the development of innovative personalized medicine to treat CRC in the future.
结肠直肠癌(CRC)是美国癌症死亡的第三大原因,预计 2023 年将有 52,000 人死亡。尽管近年来 CRC 的诊断和治疗都取得了重大进展,但 CRC 的遗传异质性--可能导致 CRC 复发和耐药性的罪魁祸首--仍然是一个难以克服的挑战。因此,在新的 CRC 治疗策略中开发更有效的疗法来克服这一挑战势在必行。遗传和表观遗传学的变化被公认为是导致 CRC 恶性肿瘤逐步发展的原因。在这篇综述中,我们重点研究了 CRC 患者中核因子(NF)-κB 信号转导的详细遗传改变信息,包括 NF-κB 家族成员及其调控因子,如精氨酸甲基转移酶 5(PRMT5)、外动力蛋白臂对接复合物亚基 2(ODAD2,又称含犰狳重复 4,ARMC4)等。此外,我们还深入探讨了不同的 CRC 研究模型,特别是患者衍生异种移植(PDX)和类器官模型,以及它们在 CRC 研究中的潜在应用。在 cBioportal for Cancer Genomics 收集的 CRC 患者中,NF-κB 信号转导元件的基因改变估计占总体基因改变的 50%以上;因此,强调了 NF-κB 在 CRC 进展中的极端重要性。因此,NF-κB 信号转导的各种基因改变可能为开发治疗 CRC 的新疗法带来巨大希望。未来的工作重点可能是利用 CRC 模型(如 PDX 或器官组织,或同源人类胚胎干细胞(hESC)衍生的结肠细胞,或人类多能干细胞(hPSC)衍生的结肠器官组织等),进一步揭示这些 NF-κB 信号转导遗传改变在 CRC 进展中的基础机制。此外,在培养皿中建立药物发现平台、开发生物数据库等,都可能为未来开发创新的个性化药物治疗 CRC 铺平道路。
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引用次数: 0
The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of Portunus trituberculatus 三疣梭子蟹精子发生过程中 Pt-DIC 和 Pt-Lamin B 的功能
IF 5.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/ijms25010112
Shuo-Yue Wang, Qiu-Meng Xiang, Junquan Zhu, Chang-Kao Mu, Chun-Lin Wang, Congcong Hou
Cytoplasmic Dynein is a multiple-subunit macromolecular motor protein involved in the transport process of cells. The Dynein intermediate chain (DIC) is one of the subunits of Dynein-1. In our previous studies, we showed that Pt-DIC may play an important role in the nuclear deformation of spermiogenesis in Portunus trituberculatus. Lamin B is essential for maintaining nuclear structure and functions. Surprisingly, Pt-Lamin B was expressed not only in the perinucleus but also in the pro-acrosome during spermiogenesis in P. trituberculatus. Studies have also shown that Dynein-1 can mediate the transport of Lamin B in mammals. Thus, to study the relationship of Pt-DIC and Pt-Lamin B in the spermatogenesis of P. trituberculatus, we knocked down the Pt-DIC gene in P. trituberculatus by RNAi. The results showed that the distribution of Pt-DIC and Pt-Lamin B in spermiogenesis was abnormal, and the colocalization was weakened. Moreover, we verified the interaction of Pt-DIC and Pt-Lamin B via coimmunoprecipitation. Therefore, our results suggested that both Pt-DIC and Pt-Lamin B were involved in the spermatogenesis of P. trituberculatus, and one of the functions of Dynein-1 is to mediate the transport of Lamin B in the spermiogenesis of P. trituberculatus.
细胞质 Dynein 是一种多亚基大分子运动蛋白,参与细胞的运输过程。Dynein中间链(DIC)是Dynein-1的亚基之一。在之前的研究中,我们发现 Pt-DIC 可能在三疣梭子蟹精子形成的核变形过程中扮演重要角色。层粘连蛋白 B 对维持核结构和功能至关重要。令人惊讶的是,在三疣梭子蟹精子形成过程中,Pt-Lamin B不仅在核周围表达,而且还在原顶体中表达。研究还表明,在哺乳动物体内,Dynein-1 可以介导 Lamin B 的运输。因此,为了研究Pt-DIC和Pt-Lamin B在三疣梭子蟹精子发生过程中的关系,我们通过RNAi敲除了三疣梭子蟹的Pt-DIC基因。结果表明,Pt-DIC和Pt-Lamin B在精子发生过程中的分布异常,共定位作用减弱。此外,我们还通过共沉淀验证了Pt-DIC和Pt-Lamin B的相互作用。因此,我们的研究结果表明,Pt-DIC和Pt-Lamin B都参与了三疣梭子蟹的精子发生,而Dynein-1的功能之一就是在三疣梭子蟹的精子发生过程中介导Lamin B的运输。
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International Journal of Molecular Sciences
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