Background: Pancreatic ductal adenocarcinoma (PDAC) is a malignant cancer and chemotherapy ineffectively treats PDAC, leading to the requirement for alternative tumor-targeted treatment. Human amniotic fluid mesenchymal stem cells (hAFMSCs) have been revealed to suppress tumor growth in various cancers and they are a strong candidate for treating PDAC.
Methods: To evaluate the effects of hAFMSCs on human pancreatic carcinoma cells (PANC1, AsPC1 and BxPC3 cell lines) and the possible mechanism involved, an in vitro cell coculture system was used. A PANC1 orthotopic xenograft mouse model was established and hAFMSCs were injected intravenously at 4 weeks post-xenograft.
Results: An in vitro coculture assay showed that hAFMSCs inhibited PANC1 cell proliferation by inducing S phase cell cycle arrest and increased cell apoptosis in a time-dependent manner. In PANC1 cells, hAFMSCs caused the downregulation of Cyclin A and Cyclin B1 as well as the upregulation of p21 (CDKN1A) at 24 h post coculture. The upregulation of pro-apoptotic factors Caspase-3/-8 and Bax at 24 h post coculture reduced the migration and invasion ability of PANC1 cells through inhibiting the epithelial-mesenchymal transition (EMT) process. In a PANC1 orthotopic xenograft mouse model, a single injection of hAFMSCs showed significant tumor growth inhibition with evidence of the modulation of cell cycle and pro-apoptotic regulatory genes and various genes involved in matrix metallopeptidase 7 (MMP7) signaling-triggered EMT process. Histopathological staining showed lower Ki67 levels in tumors from hAFMSCs-treated mice.
Conclusions: Our data demonstrated that hAFMSCs strongly inhibit PDAC cell proliferation, tumor growth and invasion, possibly by altering cell cycle arrest and MMP7 signaling-triggered EMT.
{"title":"Human amniotic fluid mesenchymal stem cells attenuate pancreatic cancer cell proliferation and tumor growth in an orthotopic xenograft mouse model.","authors":"Ying-Cheng Chen, Ying-Wei Lan, Shiaw-Min Huang, Chih-Ching Yen, Wei Chen, Wan-Ju Wu, Theresa Staniczek, Kowit-Yu Chong, Chuan-Mu Chen","doi":"10.1186/s13287-022-02910-3","DOIUrl":"10.1186/s13287-022-02910-3","url":null,"abstract":"<p><strong>Background: </strong>Pancreatic ductal adenocarcinoma (PDAC) is a malignant cancer and chemotherapy ineffectively treats PDAC, leading to the requirement for alternative tumor-targeted treatment. Human amniotic fluid mesenchymal stem cells (hAFMSCs) have been revealed to suppress tumor growth in various cancers and they are a strong candidate for treating PDAC.</p><p><strong>Methods: </strong>To evaluate the effects of hAFMSCs on human pancreatic carcinoma cells (PANC1, AsPC1 and BxPC3 cell lines) and the possible mechanism involved, an in vitro cell coculture system was used. A PANC1 orthotopic xenograft mouse model was established and hAFMSCs were injected intravenously at 4 weeks post-xenograft.</p><p><strong>Results: </strong>An in vitro coculture assay showed that hAFMSCs inhibited PANC1 cell proliferation by inducing S phase cell cycle arrest and increased cell apoptosis in a time-dependent manner. In PANC1 cells, hAFMSCs caused the downregulation of Cyclin A and Cyclin B1 as well as the upregulation of p21 (CDKN1A) at 24 h post coculture. The upregulation of pro-apoptotic factors Caspase-3/-8 and Bax at 24 h post coculture reduced the migration and invasion ability of PANC1 cells through inhibiting the epithelial-mesenchymal transition (EMT) process. In a PANC1 orthotopic xenograft mouse model, a single injection of hAFMSCs showed significant tumor growth inhibition with evidence of the modulation of cell cycle and pro-apoptotic regulatory genes and various genes involved in matrix metallopeptidase 7 (MMP7) signaling-triggered EMT process. Histopathological staining showed lower Ki67 levels in tumors from hAFMSCs-treated mice.</p><p><strong>Conclusions: </strong>Our data demonstrated that hAFMSCs strongly inhibit PDAC cell proliferation, tumor growth and invasion, possibly by altering cell cycle arrest and MMP7 signaling-triggered EMT.</p>","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":"101 1","pages":"235"},"PeriodicalIF":2.7,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73536457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-12DOI: 10.1186/s00015-022-00418-9
K. A. Stevens, Scott Ernst, D. Marty
{"title":"Coupling length: a generalized gleno-acetabular distance measurement for interpreting the size and gait of quadrupedal trackmakers","authors":"K. A. Stevens, Scott Ernst, D. Marty","doi":"10.1186/s00015-022-00418-9","DOIUrl":"https://doi.org/10.1186/s00015-022-00418-9","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":"115 1","pages":"1-27"},"PeriodicalIF":3.1,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42027794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-10DOI: 10.1186/s00015-022-00416-x
P. Mojon, E. Kaenel
{"title":"New paleontological and biostratigraphical data (calcareous nannofossils, ostracods, brachiopods), correlations and lithostratigraphic units in the Urgonian facies (latest Hauterivian-Barremian) of the Swiss and French Jura Mountains: the Falaises Member and the Saars Formation (former “Gorges de l’","authors":"P. Mojon, E. Kaenel","doi":"10.1186/s00015-022-00416-x","DOIUrl":"https://doi.org/10.1186/s00015-022-00416-x","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":" ","pages":"1-43"},"PeriodicalIF":3.1,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46358972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-03DOI: 10.1186/s00015-022-00407-y
M. Haas, Davide Carraro, D. Ventra, M. Plötze, Antoine de Haller, A. Moscariello
{"title":"Integrated stratigraphic, sedimentological and petrographical evaluation for CERN’s Future Circular Collider subsurface infrastructure (Geneva Basin, Switzerland-France)","authors":"M. Haas, Davide Carraro, D. Ventra, M. Plötze, Antoine de Haller, A. Moscariello","doi":"10.1186/s00015-022-00407-y","DOIUrl":"https://doi.org/10.1186/s00015-022-00407-y","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":" ","pages":"1-43"},"PeriodicalIF":3.1,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48047574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-22DOI: 10.1186/s00015-022-00417-w
G. Georgalis, T. Scheyer
{"title":"Crushed but not lost: a colubriform snake (Serpentes) from the Miocene Swiss Molasse, identified through the use of micro-CT scanning technology","authors":"G. Georgalis, T. Scheyer","doi":"10.1186/s00015-022-00417-w","DOIUrl":"https://doi.org/10.1186/s00015-022-00417-w","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":"115 1","pages":"1-9"},"PeriodicalIF":3.1,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45609902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-15DOI: 10.1186/s00015-022-00415-y
E. Kempf, Jörg Hermann, J. Connolly
{"title":"Serpentinite dehydration at low pressures","authors":"E. Kempf, Jörg Hermann, J. Connolly","doi":"10.1186/s00015-022-00415-y","DOIUrl":"https://doi.org/10.1186/s00015-022-00415-y","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":"115 1","pages":"1-30"},"PeriodicalIF":3.1,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47953555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-09DOI: 10.1186/s00015-022-00405-0
K. Kremer, S. Fabbri, Frederic M. Evers, Nora Schweizer, S. Wirth
{"title":"Traces of a prehistoric and potentially tsunamigenic mass movement in the sediments of Lake Thun (Switzerland)","authors":"K. Kremer, S. Fabbri, Frederic M. Evers, Nora Schweizer, S. Wirth","doi":"10.1186/s00015-022-00405-0","DOIUrl":"https://doi.org/10.1186/s00015-022-00405-0","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49040601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-26DOI: 10.1186/s00015-022-00414-z
Jonathan Pfander, F. Schlunegger, E. Serra, Natacha Gribenski, Philippos Garefalakis, N. Akçar
{"title":"Glaciofluvial sequences recording the Birrfeld Glaciation (MIS 5d–2) in the Bern area, Swiss Plateau","authors":"Jonathan Pfander, F. Schlunegger, E. Serra, Natacha Gribenski, Philippos Garefalakis, N. Akçar","doi":"10.1186/s00015-022-00414-z","DOIUrl":"https://doi.org/10.1186/s00015-022-00414-z","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":" ","pages":"1-24"},"PeriodicalIF":3.1,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48265319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-24DOI: 10.1186/s00015-022-00410-3
S. Heuberger, L. Nibourel, D. Fulda, M. Vernooij
{"title":"120 years of georesources research in Switzerland: the Swiss Geotechnical Commission (1899–2018)","authors":"S. Heuberger, L. Nibourel, D. Fulda, M. Vernooij","doi":"10.1186/s00015-022-00410-3","DOIUrl":"https://doi.org/10.1186/s00015-022-00410-3","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":" ","pages":"1-25"},"PeriodicalIF":3.1,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48807733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-24DOI: 10.1186/s00015-022-00413-0
I. Stober, F. Giovanoli, Victoria Wiebe, K. Bucher
{"title":"Deep hydrochemical section through the Central Alps: evolution of deep water in the continental upper crust and solute acquisition during water–rock-interaction along the Sedrun section of the Gotthard Base Tunnel","authors":"I. Stober, F. Giovanoli, Victoria Wiebe, K. Bucher","doi":"10.1186/s00015-022-00413-0","DOIUrl":"https://doi.org/10.1186/s00015-022-00413-0","url":null,"abstract":"","PeriodicalId":49456,"journal":{"name":"Swiss Journal of Geosciences","volume":"115 1","pages":"1-23"},"PeriodicalIF":3.1,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46439098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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