Yago Machado da Rosa, D. Zago, T. E. Oliveira, Vinícius de Anhaia Camargo, J. O. Barcellos
- This study was performed to evaluate the efficiency index of cow-calf systems, based on different feed technologies commonly employed in the Rio Grande do Sul state, southern Brazil. Hence, the efficiency of 35 cow-calf herds was evaluated by Data Envelopment Analysis (DEA). Data was collected by online survey and in-depth interviews, as well as data registers from the cow-calf systems, located in 31 municipalities. The analyzed indicators included rates of mortality, pregnancy, birth, and productivity. The feed technologies evaluated were the use of cultivated pastures during winter and summer, supplementation during winter and summer, creep feeding, deferral of natural pasture, use of grazing systems, and employee’s capacitation. Approximately 80% of the evaluated farms showed low-to-medium Efficiency Index (EI; up to 0.79 in a scale of 0 to 1), 13% presented high EI, and only 7% had very high EI (above 0.95). The use of cultivated pastures with the inclusion of protein supplementation and employees’ capacitation were highlighted as the main practices to increase efficiency of cow-calf systems in Rio Grande do Sul. The technologies identified as most impactful raise the EI of production systems from low to medium. Our results assist in decision-making in the use of feed technologies, pointing out a direction for the use of technologies that make breeding systems more efficient from a productive point of view.
{"title":"Feed technologies and efficiency in cow-calf systems","authors":"Yago Machado da Rosa, D. Zago, T. E. Oliveira, Vinícius de Anhaia Camargo, J. O. Barcellos","doi":"10.37496/rbz5120210193","DOIUrl":"https://doi.org/10.37496/rbz5120210193","url":null,"abstract":"- This study was performed to evaluate the efficiency index of cow-calf systems, based on different feed technologies commonly employed in the Rio Grande do Sul state, southern Brazil. Hence, the efficiency of 35 cow-calf herds was evaluated by Data Envelopment Analysis (DEA). Data was collected by online survey and in-depth interviews, as well as data registers from the cow-calf systems, located in 31 municipalities. The analyzed indicators included rates of mortality, pregnancy, birth, and productivity. The feed technologies evaluated were the use of cultivated pastures during winter and summer, supplementation during winter and summer, creep feeding, deferral of natural pasture, use of grazing systems, and employee’s capacitation. Approximately 80% of the evaluated farms showed low-to-medium Efficiency Index (EI; up to 0.79 in a scale of 0 to 1), 13% presented high EI, and only 7% had very high EI (above 0.95). The use of cultivated pastures with the inclusion of protein supplementation and employees’ capacitation were highlighted as the main practices to increase efficiency of cow-calf systems in Rio Grande do Sul. The technologies identified as most impactful raise the EI of production systems from low to medium. Our results assist in decision-making in the use of feed technologies, pointing out a direction for the use of technologies that make breeding systems more efficient from a productive point of view.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Warm-climate, legume-grass forage mixtures versus grass-only swards: An ecosystem services comparison","authors":"L. Sollenberger, J. Dubeux","doi":"10.37496/rbz5120210198","DOIUrl":"https://doi.org/10.37496/rbz5120210198","url":null,"abstract":"","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Escalera-Valente, J. L. Loya-Olguín, S. Martínez-González, C. Carmona-Gasca, P. U. Bautista-Rosales, Ranferi Gutiérrez-Leyva
{"title":"Evaluation of nutritional and ruminal degradability potential of sandbox (Hura crepitans L.) seeds in stabled Blackbelly sheep","authors":"F. Escalera-Valente, J. L. Loya-Olguín, S. Martínez-González, C. Carmona-Gasca, P. U. Bautista-Rosales, Ranferi Gutiérrez-Leyva","doi":"10.37496/rbz5120220012","DOIUrl":"https://doi.org/10.37496/rbz5120220012","url":null,"abstract":"","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathália Rafaela Fidelis Campos, G. S. Difante, A. Gurgel, C. M. Costa, D. B. Montagner, João Virgínio Emerenciano, L. Ítavo, C. Ítavo, R. T. D. C. Netto, E. Véras, Ana Beatriz Graciano da Costa
- This study examined the effect of different supplementation strategies for ewes in the last third of gestation, kept on a deferred Brachiaria brizantha cv. Marandu pasture, on their performance and the growth curve of their lambs. A total of 54 ewes, of no defined breed, with an average weight of 41.10±3.10 kg, were used in a completely randomized design, divided into three groups (18 ewes per treatment). In total, 61 lambs were born, 28 females and 33 males, with 3.70±0.55 and 3.68±0.69 kg birth weight, respectively. Treatments were as follows: multiple mixture concentrate supplement at 0.4% live weight (LW), and concentrate supplement at 0.8% LW. Average daily weight gain (ADG, g/animal/day) and total weight gain (TWG, kg/animal) were evaluated in ewes and lambs. Reproductive efficiency indices were measured in the ewes and biometric variables in the lambs. The ADG and TWG of ewes in the last third of gestation were higher in animals supplemented at 0.8% LW. Reproductive efficiency results were superior in ewes supplemented at 0.4% LW. The effect of ewe supplementation on lamb growth curve as estimated by the Gompertz model did not reveal differences between the curves of lambs born to ewes that received supplementation at 0.4 and 0.8% LW. However, supplementing ewes with a multiple mixture induced a reduction in the growth curve parameters of their lambs. The use of concentrated supplementation, at levels of 0.4 and 0.8% of LW, provides greater productive and reproductive performance for ewes and lambs.
{"title":"Effect of supplementation of ewes in the final third of gestation on the development of their lambs","authors":"Nathália Rafaela Fidelis Campos, G. S. Difante, A. Gurgel, C. M. Costa, D. B. Montagner, João Virgínio Emerenciano, L. Ítavo, C. Ítavo, R. T. D. C. Netto, E. Véras, Ana Beatriz Graciano da Costa","doi":"10.37496/rbz5120210094","DOIUrl":"https://doi.org/10.37496/rbz5120210094","url":null,"abstract":"- This study examined the effect of different supplementation strategies for ewes in the last third of gestation, kept on a deferred Brachiaria brizantha cv. Marandu pasture, on their performance and the growth curve of their lambs. A total of 54 ewes, of no defined breed, with an average weight of 41.10±3.10 kg, were used in a completely randomized design, divided into three groups (18 ewes per treatment). In total, 61 lambs were born, 28 females and 33 males, with 3.70±0.55 and 3.68±0.69 kg birth weight, respectively. Treatments were as follows: multiple mixture concentrate supplement at 0.4% live weight (LW), and concentrate supplement at 0.8% LW. Average daily weight gain (ADG, g/animal/day) and total weight gain (TWG, kg/animal) were evaluated in ewes and lambs. Reproductive efficiency indices were measured in the ewes and biometric variables in the lambs. The ADG and TWG of ewes in the last third of gestation were higher in animals supplemented at 0.8% LW. Reproductive efficiency results were superior in ewes supplemented at 0.4% LW. The effect of ewe supplementation on lamb growth curve as estimated by the Gompertz model did not reveal differences between the curves of lambs born to ewes that received supplementation at 0.4 and 0.8% LW. However, supplementing ewes with a multiple mixture induced a reduction in the growth curve parameters of their lambs. The use of concentrated supplementation, at levels of 0.4 and 0.8% of LW, provides greater productive and reproductive performance for ewes and lambs.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
- This study aimed to introduce R package pedSimulate, which was built to simulate pedigree, genetic merit, phenotype, and genotype data. These are amongst the most important data types that animal breeders and quantitative geneticists deal with. Twenty pedigrees with ten generations were simulated applying different combinations of three parameters: genetic variance (10 vs. 20), proportion of males selected (10 vs. 20%), and the pattern for selecting females (random, positively, or negatively based on own phenotype or parent average). Males were selected positively based on parent average. Consequently, assortative mating was applied to the pedigrees in which females were positively selected based on their own phenotype or parent average. Disassortative mating was applied to the pedigrees in which females were selected negatively based on phenotype or parent averages. Genetic gain and response to selection over generations were positive for all the pedigrees due to high selection intensity on males, mating each male with multiple females, and moderate to high heritability (0.25 and 0.40 for genetic variances 10 and 20, and the residual variance of 30). Genetic variance showed a slightly increasing trend over generations by assortative mating and lower selection intensity on males. Selection intensity on females was the same in all the pedigrees. This study provided examples of how R package pedSimulate can be adopted for pedigree, genetic merit, phenotype, and genotype data simulation in animal breeding studies. By using different functions and combining different parameters for their arguments, many scenarios can be simulated by R package pedSimulate.
-本研究旨在引入R软件包pedSimulate，该软件包用于模拟系谱、遗传优点、表型和基因型数据。这些是动物育种家和数量遗传学家处理的最重要的数据类型之一。采用遗传变异(10 vs. 20)、雄性选择比例(10 vs. 20%)和雌性选择模式(根据自身表型或亲本平均水平随机选择、正选择或负选择)这三个参数的不同组合，模拟了20个10代的家系。雄性根据亲本平均值正选择。因此，选型交配被应用到谱系中，其中女性是根据自己的表型或亲本平均水平积极选择的。异种交配应用于谱系中，其中女性根据表型或亲本平均水平被消极选择。由于对雄性的高选择强度，每只雄性与多个雌性交配，以及中等至高的遗传力(遗传方差10和20分别为0.25和0.40，残差方差为30)，所有家系的遗传增益和选择响应均为正。遗传变异在世代间表现为选择性交配和低选择强度的轻微增加趋势。在所有家系中，对雌性的选择强度相同。本研究提供了如何在动物育种研究中使用R软件包pedSimulate进行系谱、遗传优点、表型和基因型数据模拟的示例。通过使用不同的函数和组合不同的参数作为它们的实参，R包pedSimulate可以模拟许多场景。
{"title":"pedSimulate – An R package for simulating pedigree, genetic merit, phenotype, and genotype data","authors":"M. A. Nilforooshan","doi":"10.37496/rbz5120210131","DOIUrl":"https://doi.org/10.37496/rbz5120210131","url":null,"abstract":"- This study aimed to introduce R package pedSimulate, which was built to simulate pedigree, genetic merit, phenotype, and genotype data. These are amongst the most important data types that animal breeders and quantitative geneticists deal with. Twenty pedigrees with ten generations were simulated applying different combinations of three parameters: genetic variance (10 vs. 20), proportion of males selected (10 vs. 20%), and the pattern for selecting females (random, positively, or negatively based on own phenotype or parent average). Males were selected positively based on parent average. Consequently, assortative mating was applied to the pedigrees in which females were positively selected based on their own phenotype or parent average. Disassortative mating was applied to the pedigrees in which females were selected negatively based on phenotype or parent averages. Genetic gain and response to selection over generations were positive for all the pedigrees due to high selection intensity on males, mating each male with multiple females, and moderate to high heritability (0.25 and 0.40 for genetic variances 10 and 20, and the residual variance of 30). Genetic variance showed a slightly increasing trend over generations by assortative mating and lower selection intensity on males. Selection intensity on females was the same in all the pedigrees. This study provided examples of how R package pedSimulate can be adopted for pedigree, genetic merit, phenotype, and genotype data simulation in animal breeding studies. By using different functions and combining different parameters for their arguments, many scenarios can be simulated by R package pedSimulate.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Marques, H. C. Gonçalves, P. R. L. Meirelles, R. D. P. Ferreira, H. F. B. Gomes, R. Lourençon, E. P. Brito, G. Cañizares
- This study examined the replacement of maize and soybean meal with cassava chips and alfalfa grazing, respectively. Twelve lactating Anglo-Nubian goats were kept on a Panicum maximum cv. Tobiatã pasture. The experiment was laid out in a Latin square design in which the following diets were tested: ground maize + soybean meal, cassava chips + soybean meal, ground maize + alfalfa grazing, and cassava chips + alfalfa grazing. The evaluated variables were feed intake, daily weight gain, milk yield and composition, and feeding behavior of the goats as well as production costs. Cassava chips and grazed alfalfa influenced the intakes of dry matter, crude protein, neutral detergent fiber, and total digestible nutrients. However, milk yield, body weight, and body score did not change. There was no diet effect on the proportions of protein, solids-not-fat, somatic cell count, or urea nitrogen in the milk. Treatments influenced the levels of fat, lactose, and total solids in milk, with the highest fat levels achieved with diets containing alfalfa. Grazing, rumination, and idle times and time spent interacting with other goats were not influenced by diets. The evaluated feedstuffs improved feed efficiency and reduced production costs. Therefore, cassava chips and alfalfa can replace certain ingredients without impairing the production performance of goats, but rather improving the profit of the producer.
-本研究分别考察了用木薯片和苜蓿放牧代替玉米和豆粕。12只哺乳期盎格鲁-努比亚山羊饲喂Panicum maximum cv。Tobiata牧场。试验采用拉丁方试验设计，分别饲喂磨碎玉米+豆粕、木薯片+豆粕、磨碎玉米+放牧苜蓿、木薯片+放牧苜蓿。评价变量为采食量、日增重、产奶量和乳成分、采食行为和生产成本。木薯片和放牧苜蓿影响了干物质、粗蛋白质、中性洗涤纤维和总可消化营养物质的采食量。然而，产奶量、体重和体重评分没有变化。饲粮对牛奶中蛋白质、非脂肪固体、体细胞计数和尿素氮的比例没有影响。处理影响了牛奶中脂肪、乳糖和总固体含量的水平，饲料中含有苜蓿的脂肪含量最高。放牧、反刍、空闲时间和与其他山羊互动的时间不受饮食的影响。所评价的饲料提高了饲料效率，降低了生产成本。因此，木薯片和苜蓿可以替代某些成分，而不会影响山羊的生产性能，反而可以提高生产者的利润。
{"title":"Production, intake, and feeding behavior of dairy goats fed alfalfa via grazing and cassava","authors":"R. Marques, H. C. Gonçalves, P. R. L. Meirelles, R. D. P. Ferreira, H. F. B. Gomes, R. Lourençon, E. P. Brito, G. Cañizares","doi":"10.37496/rbz5120210102","DOIUrl":"https://doi.org/10.37496/rbz5120210102","url":null,"abstract":"- This study examined the replacement of maize and soybean meal with cassava chips and alfalfa grazing, respectively. Twelve lactating Anglo-Nubian goats were kept on a Panicum maximum cv. Tobiatã pasture. The experiment was laid out in a Latin square design in which the following diets were tested: ground maize + soybean meal, cassava chips + soybean meal, ground maize + alfalfa grazing, and cassava chips + alfalfa grazing. The evaluated variables were feed intake, daily weight gain, milk yield and composition, and feeding behavior of the goats as well as production costs. Cassava chips and grazed alfalfa influenced the intakes of dry matter, crude protein, neutral detergent fiber, and total digestible nutrients. However, milk yield, body weight, and body score did not change. There was no diet effect on the proportions of protein, solids-not-fat, somatic cell count, or urea nitrogen in the milk. Treatments influenced the levels of fat, lactose, and total solids in milk, with the highest fat levels achieved with diets containing alfalfa. Grazing, rumination, and idle times and time spent interacting with other goats were not influenced by diets. The evaluated feedstuffs improved feed efficiency and reduced production costs. Therefore, cassava chips and alfalfa can replace certain ingredients without impairing the production performance of goats, but rather improving the profit of the producer.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Armando Martins dos Santos, G. Pedrazza, J. Zuanazzi, M. Dall’Agnol, R. Weiler, A. Brunes, Júlio Antoniolli, D. C. Silveira
- The objective of this research was to identify the existence of root exudation of organic acid in Lotus corniculatus germplasms subjected to toxic aluminum (Al) levels and investigate the effect of this mechanism on the Al content in the root tissue and in morphological parameters of plant development. Two experiments were performed in nutrient solution to evaluate the Al accumulation and exudation of organic acids, using cultivars INIA Draco and São Gabriel and genotypes UFRGS and UF-T2. The plants were cultivated in Al-free solution, which was applied on the 45th day in half of the pots of each genotype. Root exudation was highly correlated with the reduced accumulation of Al in the root tissue (r 2 = 0.75 at 72 h). Genotype UF-T2, selected for Al tolerance, extruded 80% more oxalic acid in the presence of Al compared with the other germplasms, indicating that this mechanism is involved with Al tolerance in . This experiment showed strong evidence that L. corniculatus exhibits Type I tolerance, in which anionic channels are rapidly activated by Al exposure. Exudation of oxalic acid is likely a crucial mechanism that allows the maintenance of L. corniculatus growth when exposed to toxic Al conditions, and this characteristic should be used to identify tolerant genotypes in the future.
-本研究的目的是在毒性铝(Al)水平下鉴定白莲种质中存在根渗出有机酸的现象，并探讨这一机制对根组织中Al含量和植物发育形态参数的影响。以水稻品种INIA Draco和s o Gabriel以及基因型UFRGS和UF-T2为研究对象，在营养液中对有机酸的Al积累和分泌进行了研究。植株在无铝溶液中培养，45天在每个基因型的一半盆栽中施用无铝溶液。根分泌物与根组织中Al积累的减少高度相关(72 h时r = 0.75)。选择具有Al耐受性的基因型UF-T2在Al存在下比其他种质多挤出80%的草酸，表明这一机制与Al耐受性有关。本实验有力地证明了L. corniculatus表现出I型耐受性，其中阴离子通道被铝暴露迅速激活。草酸的分泌可能是L. corniculatus在暴露于有毒铝条件下维持生长的关键机制，这一特征应该在未来用于鉴定耐受性基因型。
{"title":"Root exudation of oxalic acid in Lotus corniculatus in response to aluminum toxicity","authors":"Armando Martins dos Santos, G. Pedrazza, J. Zuanazzi, M. Dall’Agnol, R. Weiler, A. Brunes, Júlio Antoniolli, D. C. Silveira","doi":"10.37496/rbz5120210105","DOIUrl":"https://doi.org/10.37496/rbz5120210105","url":null,"abstract":"- The objective of this research was to identify the existence of root exudation of organic acid in Lotus corniculatus germplasms subjected to toxic aluminum (Al) levels and investigate the effect of this mechanism on the Al content in the root tissue and in morphological parameters of plant development. Two experiments were performed in nutrient solution to evaluate the Al accumulation and exudation of organic acids, using cultivars INIA Draco and São Gabriel and genotypes UFRGS and UF-T2. The plants were cultivated in Al-free solution, which was applied on the 45th day in half of the pots of each genotype. Root exudation was highly correlated with the reduced accumulation of Al in the root tissue (r 2 = 0.75 at 72 h). Genotype UF-T2, selected for Al tolerance, extruded 80% more oxalic acid in the presence of Al compared with the other germplasms, indicating that this mechanism is involved with Al tolerance in . This experiment showed strong evidence that L. corniculatus exhibits Type I tolerance, in which anionic channels are rapidly activated by Al exposure. Exudation of oxalic acid is likely a crucial mechanism that allows the maintenance of L. corniculatus growth when exposed to toxic Al conditions, and this characteristic should be used to identify tolerant genotypes in the future.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ali, AS Dalab, T. Althnaian, K. Alkhodair, S. Al-Ramadan
- The objective of this study was to elucidate the optimum protocol timing of thermal manipulation (TM) during embryogenesis, which underline genetic improvement of muscle thermotolerance acquisition. For the present study, 1,440 fertile eggs were divided randomly and equally into control (37.8 °C with 56% relative humidity) and four thermally manipulated groups (TM1, TM2, TM3, and T M4) subjected to 39 °C for 18 h with 65% relative humidity daily during different embryonic periods. Then, at day 35 post-hatch, all groups were subjected to thermal challenge at 43 °C for 6 h to identify the level of thermotolerance acquisition differences between them. Hsp70 mRNA expression was evaluated by using a relative quantitatively RT-qPCR. Single nucleotide polymorphisms sequence of the Hsp70 gene was evaluated by Sanger’s sequencing method. Pectoral and thigh muscles samples were subjected to immunohistochemistry to detect Hsp70. Among TM conditions that were investigated, TM1 (39 °C for 18 h during embryonic days (ED) 7–11) induced a significant improvement in thermotolerance parameters (body temperature and T3 levels) during thermal challenge combined with an increase in the levels of Hsp70 mRNA and its protein with a high stability of nucleotide sequences in both pectoral and thigh muscles. The partial DNA sequence of Hsp70 gene in TM1 was reported, and nucleotide sequences were deposited in NCBI GenBank database with the accession numbers (MK852579) and (MK852580). Thigh muscle thermotolerance acquisition was higher than pectoral muscle during thermal challenge at 43 °C for 6 h. Thus, TM during ED7–11 may improve thermotolerance acquisition without adversely affecting performance. the and after during early and middle improves long-term thermotolerance when
{"title":"Molecular investigations of the effect of thermal manipulation during embryogenesis on muscle heat shock protein 70 and thermotolerance in broiler chickens","authors":"A. Ali, AS Dalab, T. Althnaian, K. Alkhodair, S. Al-Ramadan","doi":"10.37496/rbz5120210011","DOIUrl":"https://doi.org/10.37496/rbz5120210011","url":null,"abstract":"- The objective of this study was to elucidate the optimum protocol timing of thermal manipulation (TM) during embryogenesis, which underline genetic improvement of muscle thermotolerance acquisition. For the present study, 1,440 fertile eggs were divided randomly and equally into control (37.8 °C with 56% relative humidity) and four thermally manipulated groups (TM1, TM2, TM3, and T M4) subjected to 39 °C for 18 h with 65% relative humidity daily during different embryonic periods. Then, at day 35 post-hatch, all groups were subjected to thermal challenge at 43 °C for 6 h to identify the level of thermotolerance acquisition differences between them. Hsp70 mRNA expression was evaluated by using a relative quantitatively RT-qPCR. Single nucleotide polymorphisms sequence of the Hsp70 gene was evaluated by Sanger’s sequencing method. Pectoral and thigh muscles samples were subjected to immunohistochemistry to detect Hsp70. Among TM conditions that were investigated, TM1 (39 °C for 18 h during embryonic days (ED) 7–11) induced a significant improvement in thermotolerance parameters (body temperature and T3 levels) during thermal challenge combined with an increase in the levels of Hsp70 mRNA and its protein with a high stability of nucleotide sequences in both pectoral and thigh muscles. The partial DNA sequence of Hsp70 gene in TM1 was reported, and nucleotide sequences were deposited in NCBI GenBank database with the accession numbers (MK852579) and (MK852580). Thigh muscle thermotolerance acquisition was higher than pectoral muscle during thermal challenge at 43 °C for 6 h. Thus, TM during ED7–11 may improve thermotolerance acquisition without adversely affecting performance. the and after during early and middle improves long-term thermotolerance when","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juliana Mara de Freitas Santos, C. Andrighetto, G. Mateus, G. Lupatini, Murilo Scalet Daldon, Larissa Pereira de Lima, P. A. D. Luz, H. J. Bello
{"title":"Productive performance and ingestive behavior of crossbred heifers in integrated livestock-forest systems","authors":"Juliana Mara de Freitas Santos, C. Andrighetto, G. Mateus, G. Lupatini, Murilo Scalet Daldon, Larissa Pereira de Lima, P. A. D. Luz, H. J. Bello","doi":"10.37496/rbz5120210047","DOIUrl":"https://doi.org/10.37496/rbz5120210047","url":null,"abstract":"","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carolina Cavalcanti Fernandes Vieira, R. C. Pinto, A. F. Diógenes, A. Nunes
{"title":"Apparent digestibility of protein and essential aminoacids from commonly used feed ingredients in Brazil for juvenile shrimp Litopenaeus vannamei","authors":"Carolina Cavalcanti Fernandes Vieira, R. C. Pinto, A. F. Diógenes, A. Nunes","doi":"10.37496/rbz5120210177","DOIUrl":"https://doi.org/10.37496/rbz5120210177","url":null,"abstract":"","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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