M. G. Steiner, R. Weiler, A. Brunes, A. Mills, M. Dall’Agnol, C. Nabinger, E. A. M. D. Motta, D. C. Silveira, R. Sampaio, Gabriel Tessis
{"title":"Characterization and genetic diversity in Paspalum notatum Flügge accessions: Morphological and geographical distance","authors":"M. G. Steiner, R. Weiler, A. Brunes, A. Mills, M. Dall’Agnol, C. Nabinger, E. A. M. D. Motta, D. C. Silveira, R. Sampaio, Gabriel Tessis","doi":"10.37496/rbz5120220015","DOIUrl":"https://doi.org/10.37496/rbz5120220015","url":null,"abstract":"","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. M. Souza, M. Neumann, L. Rampim, Eduardo Rodrigues de Almeida, A.F. Matchula, F. Cristo, M. V. Faria
- The objective of this study was to evaluate the effect of harvesting corn at different maturity stages for silage production combined with four storage periods, considering nutrient loss and the chemical composition of silage of the whole plant and also of grainless plants. The experiment was performed in a completely randomized design with a factorial arrangement of six maturity stages of corn [reproductive (R); R1 to R6] and four periods of silage storage (30, 60, 90, and 120 days). At stages R1 and R2, losses by effluents occurred more intensely when compared with the more advanced stages, whereas losses by gases showed the opposite behavior, with stage R6 responsible for the greatest losses. Stage R4 showed a stable dry matter (DM) content during the storage period, 42.08%, and had the lowest levels of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) for the whole plant silage. The ADL in the silage of grainless plant of stage R4 was not the lowest, but it was close to the first reproductive stages, being an important point, since the first reproductive stages can present a vegetative fraction of better quality. The highest DM volumes produced by area occurred in the most advanced stages; however, R5 obtained a greater reduction in volume over the days of storage (20.66 kg day −1 ). The production cost was lower at the advanced stages (R4, R5, and R6) when compared with the first reproductive stages (R1, R2, and R3), and in general, during the storage period the costs did not have great variations. Based on the chemical composition of the silages, DM losses during the storage period, the stability in the costs of DM ton over the days of storage, the R4 stage was the most suitable for silage production. from 1 to 6 R1 – start of grain filling, 88 days after emergence; stage R2 – milky 99 days after emergence; stage R3 – pasty grain, 124 days after emergence; R4 – hard dough grain, 142 days after emergence; R5 – dent grain, 149 days after R6 – grain at 156 days after 120 days), for for
-本研究的目的是评价不同成熟期玉米收获对青贮生产的影响，并结合4个贮藏期，考虑全株和无粒植株青贮的营养损失和化学成分。试验采用全随机设计，按因子安排玉米6个成熟期[生殖期(R);R1 ~ R6]和4个青贮期(30、60、90、120 d)。在R1和R2阶段，与更高级的阶段相比，污水损失发生得更强烈，而气体损失表现出相反的行为，其中R6阶段损失最大。R4期青贮期间干物质(DM)含量稳定，为42.08%，中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和酸性洗涤木质素(ADL)含量最低。R4期无粒植株青贮的ADL不是最低的，但接近第一生殖期，这是一个重要的点，因为第一生殖期可以提供质量较好的营养成分。按面积计算，DM产量最高的阶段出现在最晚期;然而，R5在储存期间获得了更大的体积减少(20.66 kg day - 1)。后期(R4、R5、R6)的生产成本较繁殖初期(R1、R2、R3)低，总体而言，贮藏期的成本变化不大。综合青贮的化学成分、DM在贮藏期间的损失、DM吨成本在贮藏期间的稳定性，R4期最适合青贮生产。1 ~ 6 R1 -开始灌浆，羽化后88 d;R2期-出蛹后99天乳白色;R3期-糊状颗粒，出蛹后124天;R4—硬面团粒，出壳后142天;R5 -凹粒，149天后，R6 -凹粒，156天后，120天)，为
{"title":"Effect of storage time on the chemical composition of whole and grainless corn plant silage harvested at different maturity stages","authors":"A. M. Souza, M. Neumann, L. Rampim, Eduardo Rodrigues de Almeida, A.F. Matchula, F. Cristo, M. V. Faria","doi":"10.37496/rbz5120200180","DOIUrl":"https://doi.org/10.37496/rbz5120200180","url":null,"abstract":"- The objective of this study was to evaluate the effect of harvesting corn at different maturity stages for silage production combined with four storage periods, considering nutrient loss and the chemical composition of silage of the whole plant and also of grainless plants. The experiment was performed in a completely randomized design with a factorial arrangement of six maturity stages of corn [reproductive (R); R1 to R6] and four periods of silage storage (30, 60, 90, and 120 days). At stages R1 and R2, losses by effluents occurred more intensely when compared with the more advanced stages, whereas losses by gases showed the opposite behavior, with stage R6 responsible for the greatest losses. Stage R4 showed a stable dry matter (DM) content during the storage period, 42.08%, and had the lowest levels of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) for the whole plant silage. The ADL in the silage of grainless plant of stage R4 was not the lowest, but it was close to the first reproductive stages, being an important point, since the first reproductive stages can present a vegetative fraction of better quality. The highest DM volumes produced by area occurred in the most advanced stages; however, R5 obtained a greater reduction in volume over the days of storage (20.66 kg day −1 ). The production cost was lower at the advanced stages (R4, R5, and R6) when compared with the first reproductive stages (R1, R2, and R3), and in general, during the storage period the costs did not have great variations. Based on the chemical composition of the silages, DM losses during the storage period, the stability in the costs of DM ton over the days of storage, the R4 stage was the most suitable for silage production. from 1 to 6 R1 – start of grain filling, 88 days after emergence; stage R2 – milky 99 days after emergence; stage R3 – pasty grain, 124 days after emergence; R4 – hard dough grain, 142 days after emergence; R5 – dent grain, 149 days after R6 – grain at 156 days after 120 days), for for","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. R. Paula, N. Milani, C. Azevedo, A. A. Sedano, L. J. Souza, G. Shurson, U. S. Ruiz
- The objective of this study was to determine and compare the chemical composition; oxidation indicators; ether extract (EE) digestibility; and digestible, metabolizable, and net energy (DE, ME, and NE, respectively) content of distillers corn oil (DCO) from Brazil (CBR) and the United States (CUS), with refined (RSB) and degummed soybean oil (DSB) from Brazil offered to pigs. Fifty crossbred barrows (23.1±3.4 kg body weight) were fed a corn-soybean meal basal diet, or diets composed of 90% basal diet and 10% of one of the four oil sources (CBR, CUS, RSB, or DSB). Pigs were fed an amount of their respective experimental diets equivalent to 2.8 times the maintenance DE requirement for 9 d (sequentially 7 d for adaptation and 2 d for partial collection of feces). Distillers corn oil from Brazil contained lower linoleic acid (47.4%) than CUS (53.9%), RSB (54.2%), and DSB (51.5%), but greater contents of oleic (32.1%) and palmitic (14.6%) acids compared to CUS (27.0 and 12.9%), RSB (22.9 and 11.2%), and DSB (23.5 and 11.2%). The moisture and unsaponifiable contents of CBR (0.17 and 1.64%) and CUS (0.20 and 1.64%) were similar, but greater than the values found for RSB (0.05 and 1.20%) and DSB (0.12 and 1.02%). The anisidine value, free fatty acid content, and acidity of DCO samples were higher than soybean oils. The peroxide value and thiobarbituric reactive substances content increased in the oil samples over time. The apparent total tract digestibility (ATTD) of gross energy and the DE, ME and NE values of the oils did not differ among oil sources and ranged from 87.8 to 91.5%, and from 8280 to 8630, 8139 to 8459, and 7162 to 7444 kcal/kg, respectively. The ATTD of EE was greater in RSB and DSB than for CBR, but similar to CUS. The DCO produced in Brazil is an excellent energy source for pigs, with DE, ME, and NE values similar to those of DCO from the US and soybean oils
{"title":"Comparison of chemical composition, energy content, and digestibility of different sources of distillers corn oil and soybean oil for pigs","authors":"V. R. Paula, N. Milani, C. Azevedo, A. A. Sedano, L. J. Souza, G. Shurson, U. S. Ruiz","doi":"10.37496/rbz5120210115","DOIUrl":"https://doi.org/10.37496/rbz5120210115","url":null,"abstract":"- The objective of this study was to determine and compare the chemical composition; oxidation indicators; ether extract (EE) digestibility; and digestible, metabolizable, and net energy (DE, ME, and NE, respectively) content of distillers corn oil (DCO) from Brazil (CBR) and the United States (CUS), with refined (RSB) and degummed soybean oil (DSB) from Brazil offered to pigs. Fifty crossbred barrows (23.1±3.4 kg body weight) were fed a corn-soybean meal basal diet, or diets composed of 90% basal diet and 10% of one of the four oil sources (CBR, CUS, RSB, or DSB). Pigs were fed an amount of their respective experimental diets equivalent to 2.8 times the maintenance DE requirement for 9 d (sequentially 7 d for adaptation and 2 d for partial collection of feces). Distillers corn oil from Brazil contained lower linoleic acid (47.4%) than CUS (53.9%), RSB (54.2%), and DSB (51.5%), but greater contents of oleic (32.1%) and palmitic (14.6%) acids compared to CUS (27.0 and 12.9%), RSB (22.9 and 11.2%), and DSB (23.5 and 11.2%). The moisture and unsaponifiable contents of CBR (0.17 and 1.64%) and CUS (0.20 and 1.64%) were similar, but greater than the values found for RSB (0.05 and 1.20%) and DSB (0.12 and 1.02%). The anisidine value, free fatty acid content, and acidity of DCO samples were higher than soybean oils. The peroxide value and thiobarbituric reactive substances content increased in the oil samples over time. The apparent total tract digestibility (ATTD) of gross energy and the DE, ME and NE values of the oils did not differ among oil sources and ranged from 87.8 to 91.5%, and from 8280 to 8630, 8139 to 8459, and 7162 to 7444 kcal/kg, respectively. The ATTD of EE was greater in RSB and DSB than for CBR, but similar to CUS. The DCO produced in Brazil is an excellent energy source for pigs, with DE, ME, and NE values similar to those of DCO from the US and soybean oils","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nicole Batelli de Souza Nardelli Mendonça, Sérgio Turra Sobrane, E. Lima, David Henrique de Oliveira, Flávio de Aguiar Coelho, F. L. Cruz, L. Bernardes, R. Moreira, L. P. Naves, P. B. Rodrigues
- Two experiments were carried with broilers from 29 to 42 days of age for the nutritional evaluation of dietary chia. Thus, the nitrogen-corrected apparent metabolizable energy (AMEn) and the apparent metabolizability coefficients of gross energy and ether extract of chia seeds and oil, toasted soybean grain (TSG), and soybean oil were determined in the experiment I, by total excreta collection method, using 120 broilers. Each experimental diet (reference diet [RD] and four diets with the tested feedstuffs) was evaluated in eight replicates of three broilers. The TSG and chia seeds replaced the RD in 250 g/kg, whereas soybean and chia oils replaced the RD in 100 g/kg. Simultaneously, a second experiment was carried subdivided into two trials. In the performance trial, we evaluated the dietary feedstuffs effects on performance, carcass and cut yields, blood parameters, and activity of lipogenic enzymes. The nutrient metabolizability coefficients and AMEn were evaluated in the metabolism trial. The AMEn values of 37.49, 37.35, 15.85, and 8.43 MJ/kg of dry matter were determined for chia oil, soybean oil, TSG, and chia seeds, respectively (experiment I). In the second experiment, the best feed conversion was observed in broilers fed diets containing chia oil and TSG. However, the diet formulated with chia seeds worsened broiler feed conversion, exhibited the smaller energy value and apparent metabolizability coefficient of the ether extract, and increased the activity of the malic enzyme and serum total cholesterol level. There was no difference for glucose-6-phosphate dehydrogenase activity and high-density lipoprotein cholesterol level. In general, chia oil showed to be efficient in replacing soybean oil in broiler diets.
{"title":"Nutritional evaluation of chia (Salvia hispanica) seeds and oil in broiler diets","authors":"Nicole Batelli de Souza Nardelli Mendonça, Sérgio Turra Sobrane, E. Lima, David Henrique de Oliveira, Flávio de Aguiar Coelho, F. L. Cruz, L. Bernardes, R. Moreira, L. P. Naves, P. B. Rodrigues","doi":"10.37496/rbz5120220005","DOIUrl":"https://doi.org/10.37496/rbz5120220005","url":null,"abstract":"- Two experiments were carried with broilers from 29 to 42 days of age for the nutritional evaluation of dietary chia. Thus, the nitrogen-corrected apparent metabolizable energy (AMEn) and the apparent metabolizability coefficients of gross energy and ether extract of chia seeds and oil, toasted soybean grain (TSG), and soybean oil were determined in the experiment I, by total excreta collection method, using 120 broilers. Each experimental diet (reference diet [RD] and four diets with the tested feedstuffs) was evaluated in eight replicates of three broilers. The TSG and chia seeds replaced the RD in 250 g/kg, whereas soybean and chia oils replaced the RD in 100 g/kg. Simultaneously, a second experiment was carried subdivided into two trials. In the performance trial, we evaluated the dietary feedstuffs effects on performance, carcass and cut yields, blood parameters, and activity of lipogenic enzymes. The nutrient metabolizability coefficients and AMEn were evaluated in the metabolism trial. The AMEn values of 37.49, 37.35, 15.85, and 8.43 MJ/kg of dry matter were determined for chia oil, soybean oil, TSG, and chia seeds, respectively (experiment I). In the second experiment, the best feed conversion was observed in broilers fed diets containing chia oil and TSG. However, the diet formulated with chia seeds worsened broiler feed conversion, exhibited the smaller energy value and apparent metabolizability coefficient of the ether extract, and increased the activity of the malic enzyme and serum total cholesterol level. There was no difference for glucose-6-phosphate dehydrogenase activity and high-density lipoprotein cholesterol level. In general, chia oil showed to be efficient in replacing soybean oil in broiler diets.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
- The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.
本研究的目的是探讨重组脂联素对鸡肝细胞的影响。通过PCR扩增鸡脂联素全长基因，将其克隆到载体pET-32a中，转化至大肠杆菌BL21。利用SDS-PAGE对表达的重组蛋白进行纯度检测和分析。用1 mM IPTG在30℃下诱导3 h，用Ni-NTA亲和层析纯化重组硫氧还毒素-脂联素融合蛋白。成功地在细菌系统中表达和纯化了鸡脂联素。此外，鸡重组脂联素表明，它改善了棕榈酸和油酸诱导的脂肪生成，其中可能涉及β-氧化的增加和脂肪生成相关基因的减少。综上所述，鸡重组脂联素增强了LMH细胞的脂肪酸代谢。
{"title":"Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells","authors":"Yi-Ru Zhuang, Yuan-Yu Lin","doi":"10.37496/rbz5120220087","DOIUrl":"https://doi.org/10.37496/rbz5120220087","url":null,"abstract":"- The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marta Maria dos Santos, T. C. Costa, G. D. Ramírez-Zamudio, K. B. Nascimento, M. Gionbelli, M. Duarte
- The productive traits of beef cattle are orchestrated by their genetics, postnatal environmental conditions, and also by the intrauterine background. Both under- or overnutrition, as specific dietary components, are able to promote persistent effects on the offspring. This occurs because dietary factors act not only affecting the availability of substrates for fetal anabolism and oxidative metabolism, but also as signals that regulate several events toward fetal development. Therefore, this study aimed to summarize the gestational nutrition effects on the offspring performance and meat quality in a long term. Overall, studies have shown that many of these alterations are under the control of epigenetic mechanisms, as DNA methylation, histones modification, and non-coding RNA. The current knowledge has indicated that the fetal programming responses are dependent on the window of fetal development in which the dietary treatment is applied, the intensity of maternal nutritional stimuli, and the treatment application length. Collectively, studies demonstrated that muscle cell hyperplasia is impaired when maternal requirements were not achieved in the second third of gestation, which limits the formation of a greater number of muscle fibers and the offspring growth potential in a long term. Changes in muscle fibers metabolism and in collagen content were also reported as consequence of a dietary perturbation during pregnancy. In contrast, a maternal overnutrition during the late pregnancy has been associated with beneficial responses on meat quality. In summary, ensuring an adequate maternal environment during the fetal development is crucial to enhance the productive responses in beef cattle operations.
{"title":"Prenatal origins of productivity and quality of beef","authors":"Marta Maria dos Santos, T. C. Costa, G. D. Ramírez-Zamudio, K. B. Nascimento, M. Gionbelli, M. Duarte","doi":"10.37496/rbz5120220061","DOIUrl":"https://doi.org/10.37496/rbz5120220061","url":null,"abstract":"- The productive traits of beef cattle are orchestrated by their genetics, postnatal environmental conditions, and also by the intrauterine background. Both under- or overnutrition, as specific dietary components, are able to promote persistent effects on the offspring. This occurs because dietary factors act not only affecting the availability of substrates for fetal anabolism and oxidative metabolism, but also as signals that regulate several events toward fetal development. Therefore, this study aimed to summarize the gestational nutrition effects on the offspring performance and meat quality in a long term. Overall, studies have shown that many of these alterations are under the control of epigenetic mechanisms, as DNA methylation, histones modification, and non-coding RNA. The current knowledge has indicated that the fetal programming responses are dependent on the window of fetal development in which the dietary treatment is applied, the intensity of maternal nutritional stimuli, and the treatment application length. Collectively, studies demonstrated that muscle cell hyperplasia is impaired when maternal requirements were not achieved in the second third of gestation, which limits the formation of a greater number of muscle fibers and the offspring growth potential in a long term. Changes in muscle fibers metabolism and in collagen content were also reported as consequence of a dietary perturbation during pregnancy. In contrast, a maternal overnutrition during the late pregnancy has been associated with beneficial responses on meat quality. In summary, ensuring an adequate maternal environment during the fetal development is crucial to enhance the productive responses in beef cattle operations.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ferreira, M. A. P. Rodriguez, L. Oliveira, C. Maranhão, N. Oliveira, C. C. S. Carvalho, Marcos Vinícius Ramos Afonso, Mariana Rabelo Madureira
The objective was to study the stress level in dogs during grooming services in a pet shop. A total of 55 grooming services carried out in a pet shop in the city of Janaúba, MG, were evaluated and divided into four categories according to sex and body weight. The flowchart of the grooming process contained six steps that began with the transportation of dogs to the pet shop and finished with their return to the place of origin. Behavioral, physiological, and blood component evaluations were performed at different steps of the process. Changes in dog behavior and physiological parameters were observed primarily upon arrival at the pet shop and during drying. Employee characteristics also influenced dogs’ behaviors. However, dogs were able to thermoregulate and maintain the homeostasis of leukocytes, glucose and cortisol in the blood.
{"title":"Stress in dogs during grooming in a pet shop","authors":"M. Ferreira, M. A. P. Rodriguez, L. Oliveira, C. Maranhão, N. Oliveira, C. C. S. Carvalho, Marcos Vinícius Ramos Afonso, Mariana Rabelo Madureira","doi":"10.37496/rbz5120200154","DOIUrl":"https://doi.org/10.37496/rbz5120200154","url":null,"abstract":"The objective was to study the stress level in dogs during grooming services in a pet shop. A total of 55 grooming services carried out in a pet shop in the city of Janaúba, MG, were evaluated and divided into four categories according to sex and body weight. The flowchart of the grooming process contained six steps that began with the transportation of dogs to the pet shop and finished with their return to the place of origin. Behavioral, physiological, and blood component evaluations were performed at different steps of the process. Changes in dog behavior and physiological parameters were observed primarily upon arrival at the pet shop and during drying. Employee characteristics also influenced dogs’ behaviors. However, dogs were able to thermoregulate and maintain the homeostasis of leukocytes, glucose and cortisol in the blood.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Genova, A. D. B. Melo, P. Rupolo, R. F. Macedo, J. R. Engracia, S. T. Carvalho, L. Faucitano, L. B. Costa, P. Carvalho
- The aim of this study was to assess the addition of intestinal alkaline phosphatase (IAP) to diets on the count of bacterial populations, pH of digestive organ contents, histopathological description, proinflammatory markers, hepatic glycogen reserve, and diarrhoea incidence of piglets challenged with Escherichia coli . Sixty-four crossbred piglets (7.16±0.28 kg body weight, 25-days-old) were assigned to four treatments in a completely randomised block design: negative control (NC), NC + antibiotic (ANT), NC + 15 mg IAP, or NC + 30 mg IAP kg −1 of diet, eight replications of two piglets per experimental unit. All piglets were orally challenged with 6 mL of a solution containing enterotoxigenic Escherichia coli K88 at 10 6 CFU mL −1 at 15 days of experimentation. The study lasted for 19 days. At the end of the experimental period, the piglets were slaughtered (six animals per treatment). Enterobacteriaceae in caecum and colon was lower in piglets on 30 mg IAP than with ANT and NC, ANT or 15 mg IAP, respectively. Enterobacteriaceae adhered to the mesenteric lymph nodes (MLN) was greater in piglets fed ANT than the other treatments. Lactic acid bacteria (LAB) count in caecum was greater in piglets fed NC and ANT. In MLN, LAB count was greater in ANT and 30 mg IAP-fed piglets compared with 15 mg IAP. Piglets in 30 mg IAP in diet showed a tendency for lowering tissue necrosis compared with NC or ANT. Piglets fed 30 mg IAP showed a reduction in diarrhoea incidence in the pre- and post-challenge compared with 15 mg IAP and all other treatments, respectively. Based on the criteria, addition of 30 mg IAP to diet inhibits Enterobacteriaceae population and suggests a potential effect in mitigating intestinal injuries, as observed in piglets in the NC for some of the parameters investigated.
{"title":"New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)","authors":"J. Genova, A. D. B. Melo, P. Rupolo, R. F. Macedo, J. R. Engracia, S. T. Carvalho, L. Faucitano, L. B. Costa, P. Carvalho","doi":"10.37496/rbz5120210144","DOIUrl":"https://doi.org/10.37496/rbz5120210144","url":null,"abstract":"- The aim of this study was to assess the addition of intestinal alkaline phosphatase (IAP) to diets on the count of bacterial populations, pH of digestive organ contents, histopathological description, proinflammatory markers, hepatic glycogen reserve, and diarrhoea incidence of piglets challenged with Escherichia coli . Sixty-four crossbred piglets (7.16±0.28 kg body weight, 25-days-old) were assigned to four treatments in a completely randomised block design: negative control (NC), NC + antibiotic (ANT), NC + 15 mg IAP, or NC + 30 mg IAP kg −1 of diet, eight replications of two piglets per experimental unit. All piglets were orally challenged with 6 mL of a solution containing enterotoxigenic Escherichia coli K88 at 10 6 CFU mL −1 at 15 days of experimentation. The study lasted for 19 days. At the end of the experimental period, the piglets were slaughtered (six animals per treatment). Enterobacteriaceae in caecum and colon was lower in piglets on 30 mg IAP than with ANT and NC, ANT or 15 mg IAP, respectively. Enterobacteriaceae adhered to the mesenteric lymph nodes (MLN) was greater in piglets fed ANT than the other treatments. Lactic acid bacteria (LAB) count in caecum was greater in piglets fed NC and ANT. In MLN, LAB count was greater in ANT and 30 mg IAP-fed piglets compared with 15 mg IAP. Piglets in 30 mg IAP in diet showed a tendency for lowering tissue necrosis compared with NC or ANT. Piglets fed 30 mg IAP showed a reduction in diarrhoea incidence in the pre- and post-challenge compared with 15 mg IAP and all other treatments, respectively. Based on the criteria, addition of 30 mg IAP to diet inhibits Enterobacteriaceae population and suggests a potential effect in mitigating intestinal injuries, as observed in piglets in the NC for some of the parameters investigated.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniel Anderson de Souza Melo, J. M. Santiago, Jorge Eduardo Cavalcante Lucena, Luís Artur Cordeiro Costa, A. Ribeiro, Andreza Correia da Silva, I. Gonzaga
- The objective of this study was to assess a training protocol employed in the Brazilian Northeast region for fitness conditioning of vaquejada horses. For 12 months, 24 Quarter Horses were evaluated under a completely randomized split-plot experimental design in which the plots comprised three age groups: horses at two, three, and four years of age. The split plots were made up of six fitness tests carried out every other month. The fitness test protocol consisted of five levels of protocol exercises on a standard vaquejada track. Prior to the tests with fasted animals, we collected blood samples to determine muscle enzymes (aspartate aminotransferase, creatine kinase, and lactate dehydrogenase). During the tests, heart rate, speed, and distance run were recorded using a heart rate monitor. Next, the results were used to calculate speed at which each horse reached 150 bpm (V 150 ), speed at which each horse reached 200 bpm (V 200 ), maximum heart rate (HR max ), maximum speed (V max ), recovery time needed for the heart rate of horses to return to half the maximum value reached during the fitness tests (HR 50% ), and recovery time needed for the heart rate of horses to return to baseline values (HR basal ). No difference was found among the age groups for V 150 , HR max , V max , HR 50% , HR basal , or muscle enzymes. By the final stage of training, the V 200 of the three-year-old horses was higher than that of the four-year-old foals. During training, all groups exhibited increases in serum concentrations of muscle enzymes and reductions in efficiency to recover heart rate after exercise. The training protocol assessed is unable to maintain proper fitness for competitions throughout the year.
{"title":"Assessment of a vaquejada horse training protocol based on laboratory clinical parameters","authors":"Daniel Anderson de Souza Melo, J. M. Santiago, Jorge Eduardo Cavalcante Lucena, Luís Artur Cordeiro Costa, A. Ribeiro, Andreza Correia da Silva, I. Gonzaga","doi":"10.37496/rbz5120210111","DOIUrl":"https://doi.org/10.37496/rbz5120210111","url":null,"abstract":"- The objective of this study was to assess a training protocol employed in the Brazilian Northeast region for fitness conditioning of vaquejada horses. For 12 months, 24 Quarter Horses were evaluated under a completely randomized split-plot experimental design in which the plots comprised three age groups: horses at two, three, and four years of age. The split plots were made up of six fitness tests carried out every other month. The fitness test protocol consisted of five levels of protocol exercises on a standard vaquejada track. Prior to the tests with fasted animals, we collected blood samples to determine muscle enzymes (aspartate aminotransferase, creatine kinase, and lactate dehydrogenase). During the tests, heart rate, speed, and distance run were recorded using a heart rate monitor. Next, the results were used to calculate speed at which each horse reached 150 bpm (V 150 ), speed at which each horse reached 200 bpm (V 200 ), maximum heart rate (HR max ), maximum speed (V max ), recovery time needed for the heart rate of horses to return to half the maximum value reached during the fitness tests (HR 50% ), and recovery time needed for the heart rate of horses to return to baseline values (HR basal ). No difference was found among the age groups for V 150 , HR max , V max , HR 50% , HR basal , or muscle enzymes. By the final stage of training, the V 200 of the three-year-old horses was higher than that of the four-year-old foals. During training, all groups exhibited increases in serum concentrations of muscle enzymes and reductions in efficiency to recover heart rate after exercise. The training protocol assessed is unable to maintain proper fitness for competitions throughout the year.","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernanda Ferreira dos Santos, L. Brochine, R. Nacimento, Flávia Mallaco Moreira, A. Gameiro, S. B. Gallo
{"title":"Economic performance of high-energy diets and supplementation with chromium propionate or calcium salts of palm oil in ewes’ production","authors":"Fernanda Ferreira dos Santos, L. Brochine, R. Nacimento, Flávia Mallaco Moreira, A. Gameiro, S. B. Gallo","doi":"10.37496/rbz5120210063","DOIUrl":"https://doi.org/10.37496/rbz5120210063","url":null,"abstract":"","PeriodicalId":49614,"journal":{"name":"Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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