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Genetic evaluation of oocyte and embryo production in dairy Gir cattle using repeatability and random regression models 利用可重复性和随机回归模型对奶牛卵母细胞和胚胎生产进行遗传评价
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.37496/rbz5120220017
Giovani Luis Feltes, R. Negri, F. Raidan, L. Feres, V. Ribeiro, J. A. Cobuci
- The objective of this work is to estimate genetic parameters and breeding values to improve embryo and oocyte production, using repeatability and random regression models (RRM) for Gir dairy cattle. We used 11,398 records of ovum pick-up from 1,747 dairy Gir donors and evaluated sixteen different models: the traditional repeatability model and fifteen RRM, each of which considered a different combination of Legendre polynomial regressors to describe the additive genetic and permanent environment effects. The 4G1P model (four regressors for the genetic effect and one regressor for the permanent environment effect) is the most suitable model to analyze the number of viable and total oocytes, while the 3G1P is the best model to analyze the number of cleaved and viable embryos, according to the values of the Akaike information criterion (AIC) and the Bayesian information criterion (BIC). The heritability estimated with the RRM was higher than that estimated with the repeatability model. The high repeatability reported for oocyte and embryo count traits indicates that donors, which had high oocyte and embryo counts in the first ovum pick-up, should maintain this result in the next ovum pick-up. Genetic correlations between adjacent ages were high and positive, while genetic correlations between extreme ages were weak. We observed a reranking of the top sires and females (heifers and cows) over the period evaluated. The reliability of the estimated breeding values by RRM showed changes across age, and the expected genetic gains by RRM are larger. This shows that RRM is most suitable alternative for the evaluation and selection of oocyte and embryo count traits.
-本工作的目的是利用可重复性和随机回归模型(RRM)估计遗传参数和育种价值,以提高吉尔奶牛的胚胎和卵母细胞产量。我们使用了1,747名奶牛女孩捐赠者的11,398份卵子采集记录,并评估了16种不同的模型:传统的可重复性模型和15种RRM模型,每种模型都考虑了不同的Legendre多项式回归量组合来描述可加性遗传和永久环境效应。从赤池信息准则(Akaike information criterion, AIC)和贝叶斯信息准则(Bayesian information criterion, BIC)的值来看,4G1P模型(遗传效应的4个回归量和永久环境效应的1个回归量)最适合分析活卵数和总卵母细胞数,3G1P模型最适合分析裂卵数和活卵数。RRM估计的遗传力高于重复性模型估计的遗传力。报告的卵母细胞和胚胎计数特征的高重复性表明,在第一次取卵时具有高卵母细胞和胚胎计数的供者在下一次取卵时应保持这一结果。相邻年龄间遗传相关性高且呈正相关,极端年龄间遗传相关性弱。在评估期间,我们观察到排名靠前的母畜(小母牛和母牛)的重新排名。RRM估计育种值的可靠性随年龄的变化而变化,RRM的预期遗传收益较大。这表明RRM是评价和选择卵母细胞和胚胎数量性状最合适的选择。
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引用次数: 1
Growth performance and meat quality of feedlot steers fed diets with or without natural feed additive 饲粮中添加或不添加天然饲料添加剂对育肥场阉牛生长性能和肉品质的影响
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.37496/rbz5120210096
R. L. Missio, Renato Guedes Gaspar, W. Paris, F. Kuss, G. B. Souto, Marcelo Machado Severo, L. F. G. D. Menezes
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引用次数: 1
Relationships of conformation traits with somatic cell score in Nubian goats 努比亚山羊构象性状与体细胞评分的关系
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-11-18 DOI: 10.37496/rbz5020210005
C. Ángel-Sahagún, Sonia Betzabeth Campos-Castillo, A. Gutiérrez-Chávez, A. A. Lechuga-Arana, J. A. Hernández-Marín, Lisa Shepard, H. Montaldo, M. Valencia-Posadas
ABSTRACT Records of 3716 Nubian goats from the United States (US) were analyzed to estimate relationships between fourteen conformation traits (CT) with lactation average somatic cell score (ASCS). To analyze ASCS, a mixed model was implemented. Linear and quadratic effects of CT traits, days in milk (DIM), and kidding age in months (KA) were considered as fixed covariates, and herd-year (HY) of kidding as a random effect. Correlation coefficients between CT traits and ASCS adjusted for HY and linear and quadratic KA effects were also obtained. The average ± standard deviations for ASCS, DIM, and milk yield were 5.17±0.54 Log2, equivalent to 451.3 cells × 103/mL, 266.3±52.1 days, and 776.3±280.4 kg per lactation, respectively. Significant non-linear relationships with an intermediate maximum were found between ASCS with teat diameter and medial suspensory ligament, while linear relationships were observed with stature, strength, rump width, fore udder attachment, udder depth, teat diameter, teat placement, and medial suspensory ligament. The model explained 53.7% of the ASCS variability, but the contribution of each type variable to increase the coefficient of determination was low (<0.52%). Herd-year explained a large proportion of the variation of ASCS (38.4%). All estimated correlations between CT and ASCS had low values, from −0.04 to 0.11, but most were significant. The results of this study show that conformation traits have few opportunities to contribute phenotypically to assess somatic cell score in Nubian goats.
摘要对来自美国的3716只努比亚山羊的记录进行了分析,以估计14个构象特征(CT)与泌乳平均体细胞评分(ASCS)之间的关系。为了分析ASCS,实现了一个混合模型。CT性状、产奶天数(DIM)和月龄(KA)的线性和二次效应被认为是固定的协变量,开玩笑的群体年(HY)被认为是随机效应。还获得了经HY和线性和二次KA效应调整后的CT性状和ASCS之间的相关系数。ASCS、DIM和产奶量的平均±标准偏差分别为5.17±0.54 Log2,相当于每次泌乳451.3个细胞×103/mL、266.3±52.1天和776.3±280.4 kg。在具有乳头直径的ASCS和内侧悬吊韧带之间发现了具有中间最大值的显著非线性关系,而与身材、力量、臀部宽度、前乳附着、乳房深度、乳头直径、乳头位置和内侧悬吊膜之间则观察到了线性关系。该模型解释了53.7%的ASCS变异性,但每个类型变量对增加决定系数的贡献都很低(<0.52%)。群体年解释了很大一部分ASCS变异(38.4%)。CT和ASCS之间的所有估计相关性都很低,从−0.04到0.11,但大多数都是显著的。这项研究的结果表明,构象特征很少有机会对努比亚山羊体细胞评分的表型做出贡献。
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引用次数: 0
Polymorphism in the BIEC2-808543 locus and its association with growth curve in Brasileiro de Hipismo horse breed 巴西赛马品种BIEC2-808543基因座的多态性及其与生长曲线的相关性
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-11-18 DOI: 10.37496/rbz5020200214
Érica Cristina Xisto da Costa, R. Vieira, L. Glória, M. Soares
ABSTRACT Our objective was to evaluate whether the single nucleotide polymorphism (SNP) BIEC2-808543, identified in some horse breeds, also occurs in the Brasileiro de Hipismo (BH) breed. In addition, we verified if this SNP is related to the growth curve profile of these animals for the variables body mass, height at withers, and height at croup, using nonlinear mixed models. For the DNA isolation, we collected blood samples from 167 young BH horses. We obtained the genotypes of these animals [...]
摘要:我们的目的是评估在一些马品种中发现的单核苷酸多态性(SNP)BIEC2-808543是否也发生在Brasileiro de Hipismo(BH)品种中。此外,我们使用非线性混合模型验证了该SNP是否与这些动物的生长曲线轮廓有关,包括体重、肩颈高度和臀部高度。为了进行DNA分离,我们采集了167匹BH幼马的血液样本。我们获得了这些动物的基因型[…]
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引用次数: 0
Effects of metabolic changes produced in ewes with subclinical pregnancy toxemia over reproductive parameters 亚临床妊娠毒血症母羊代谢变化对生殖参数的影响
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-10-15 DOI: 10.37496/rbz5020200213
Mayra Cecilia Abreu-Palermo, Pablo Rodríguez-Gamarra, Sofía Perini-Perera, J. Acosta-Dibarrat, Alejandro Benech-Gulla, J. González-Montaña, L. Cal-Pereyra
ABSTRACT The objective of this work was to determine the repercussion of the metabolic changes that occur in induced subclinical pregnancy toxemia in single- or twin-bearing ewes on the duration of gestation, type and length of lambing, and placental expulsion time. Fifty-one adult Corriedale ewes with a known gestation date and fed in the wild were randomly divided at day 145 of gestation into four groups. Group A included single-bearing ewes, and C twin-bearing ewes, all of which were fed [...]
摘要本研究的目的是确定单胎或双胎母羊在诱发亚临床妊娠毒血症时发生的代谢变化对妊娠期、产羔类型和长度以及胎盘排出时间的影响。在妊娠第145天,将51只已知妊娠日期并在野外饲养的成年Corriedale母羊随机分为四组。A组为单胎母羊,C组为双胎母羊
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引用次数: 3
Soybean meal from damaged grains replacing standard soybean meal in diets of feedlot lambs 饲育羔羊日粮中受损谷物豆粕替代标准豆粕的试验研究
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-10-15 DOI: 10.37496/rbz5020190268
Laura Barbosa de Carvalho, M. Abreu, M. A. B. Caneppele, G. Corrêa, B. Vieira, L. R. Lima, W. Guerra, L. S. Cabral
ABSTRACT Two studies were carried out to evaluate the effects of levels of soybean meal produced from damaged soybean grains replacing normal soybean meal on in vitro and in situ digestibility of ruminant diets as well as on intake, digestibility, and animal performance of growing/finishing lambs. In trial 1, we evaluated the in vitro digestibility of each soybean meals (normal and damaged), as well as diets containing levels of the damaged replacing the normal (0, 333, 667 and 1,000 g.kg−1) soybean meal on kinetic parameters of in vitro cumulative gas production, in vitro dry matter and crude protein digestibility, in situ rumen-degradable protein, rumen-undegradable protein, and in vitro intestinal digestibility of rumen-undegradable protein. In trial 2, we used 48 growing/finishing lambs to evaluate the effects of damaged soybean meal levels (0, 333, 667, and 1,000 g.kg−1) replacing normal soybean meal in feedlot diets (20:80 roughage:concentrate ratio) on intake and digestibility of nutrients and on animal performance. The damaged soybean meal presented lower values for total gas production and in vitro dry matter digestibility than normal soybean meal. Higher rumen-undegradable protein was estimated for damaged soybean meal than for the normal and consequently lower rumen-degradable protein for damaged compared to normal. Because of the lower rumen-degradable protein, damaged soybean meal promoted lower in vitro ammonium nitrogen (NH3-N) concentrations than the normal in feedlot diets. In the in vivo trial, there were no effects of damaged soybean meal levels in the diets on intake and digestibility of nutrients (dry matter, organic matter, crude protein, and fiber) as well as on total weight gain, average daily gain, carcass yield, or feeding efficiency. Thus, damaged soybean meal can fully replace the normal one in lamb feedlot diets (in up to of 1,000 g.kg−1 of the normal soybean meal) without causing adverse effects on intake and digestibility of nutrients and on animal performance.
摘要本试验旨在研究用受损豆粕替代正常豆粕对反刍动物饲粮体外和原位消化率以及生长/育肥羔羊采食量、消化率和动物生产性能的影响。试验1通过体外累积产气量、体外干物质和粗蛋白质消化率、瘤胃原位降解蛋白、瘤胃不可降解蛋白和瘤胃不可降解蛋白的体外肠道消化率等动力学参数,对每种豆粕(正常和受损)以及受损豆粕替代正常(0、333、667和1000 g.kg−1)豆粕的体外消化率进行了评估。在试验2中,我们选用48只生长/肥育羔羊,评估在饲粮(粗精比20:80)中,用受损豆粕水平(0、333、667和1000 g.kg−1)替代正常豆粕对营养物质的摄入和消化率以及动物生产性能的影响。受损豆粕的总产气量和体外干物质消化率低于正常豆粕。据估计,受损豆粕的瘤胃不可降解蛋白高于正常豆粕,因此受损豆粕的瘤胃可降解蛋白低于正常豆粕。由于受损豆粕中瘤胃可降解蛋白含量较低,导致饲粮中氨氮(NH3-N)浓度低于正常水平。在体内试验中,饲粮中受损豆粕水平对营养物质(干物质、有机物、粗蛋白质和纤维)的摄入量和消化率以及总增重、平均日增重、胴体产量或饲养效率均无影响。因此,在羔羊饲粮中,受损豆粕可以完全替代正常豆粕(正常豆粕可达1,000 g.kg - 1),而不会对营养物质的摄入和消化率以及动物生产性能产生不利影响。
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引用次数: 1
Diet energy levels and temperature affect the size of the fat milk globule in dairy goats 日粮能量水平和温度对奶山羊脂肪乳球大小的影响
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-10-04 DOI: 10.37496/rbz5020200145
R. Costa, David Kleberson Rodrigues de Azevedo, N. Ribeiro, Mikael Leal Cabral Menezes de Amorim, R. R. Guerra, A. Sant’Ana, I. Altomonte, M. Martini
ABSTRACT The study aimed to verify the effect of diet and environmental temperature on traits of milk fat globules (MFG) of goats. The experiment was conducted in climatic chambers, where we housed 12 Alpine goats with a mean age of 4.02±1.78 years, live weight of 41.8±4.59 kg, and average milk production of 2.16±0.59 kg. The animals were subjected to two different controlled temperatures, T1 = 26 ℃ (thermoneutral) and T2 = 34 ℃ (stress), and diets with different energy levels (low, medium, and high). A milk sample of each animal was collected at 6.00 h, coinciding with milking. The effect of temperature and diet was verified on MFG. The highest MFG was observed at 26 ℃ and medium energy diet. The MFG reached lower values with the diet of medium energy and high temperature (34 ℃). On average, 35% of MFG is smaller than 2 μm, 50% is medium in size (2-5 μm), and 15% is large (>5 μm), with a maximum size of 9.57 μm. The higher prevalence of medium-sized MFG is indicative of excellent milk digestibility. The increase in dietary energy levels promoted both the fat and diameter of fat globules. The higher fat and the larger globules would positively affect the cheese-making aptitude and make it suitable for production of hard cheeses. The increase in dietary energy levels for goats promotes an increase in the diameter of fat globules and milk fat (%), essential traits to the cheese industry.
摘要本试验旨在验证饲粮和环境温度对山羊乳脂球(MFG)性状的影响。试验在气候箱中饲养12只高山山羊,平均年龄4.02±1.78岁,活重41.8±4.59 kg,平均产奶量2.16±0.59 kg。试验动物分别处于T1 = 26℃(热中性)和T2 = 34℃(应激)两种不同的控制温度和低、中、高3种不同能量水平的饲粮中。每只动物于挤奶时间6时采集一乳样。验证了温度和日粮对MFG的影响。在26℃和中能量日粮时,MFG最高。在中能量、高温(34℃)日粮条件下,MFG值较低。平均35%的MFG尺寸小于2 μm, 50%为中尺寸(2 ~ 5 μm), 15%为大尺寸(5 ~ 5 μm),最大尺寸为9.57 μm。中型乳牛的较高患病率表明牛奶消化率良好。膳食能量水平的增加促进了脂肪和脂肪球直径的增加。较高的脂肪和较大的球将积极影响奶酪的制作能力,使其适合生产硬奶酪。山羊日粮能量水平的提高促进了脂肪球直径和乳脂(%)的增加,这是奶酪工业的基本性状。
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引用次数: 0
Practical procedures to body weight estimation and correction factors applied to Campolina horses 实用程序体重估计和修正系数适用于坎波利纳马
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-09-23 DOI: 10.37496/rbz5020200047
F. A. C. D. Souza, Glaciane Lopes Teixeira, T. J. Fernandes, F. R. Muniz, Fabiana Oliveira Cunha, S. L. Meirelles, J. A. Muniz, R. S. Moura
ABSTRACT The objective of this study was to evaluate the accuracy of the following six body weight (BW) estimation methods in Campolina (CAM) horses: A - weight tape placed at three different positions on the animal’s thorax; B - Crevat and Quetelet’s formula; C - Hall’s formula; D - Hintz and Griffiths’s table; E - Santos’s table; and F - Cintra’s formula. A total of 380 CAM horses were separated according to sex, age class, and gestational stage and evaluated. To determine their accuracy, weights measured on a scale and weight estimates of the six methods were compared by paired t-test, mean prediction error (MPE), and coefficient of determination (R2), using R software. The predictive capacity of method F was lower in the 6-12 months age class, so this formula is not indicated. The BW was overestimated compared with the actual weight by methods A (with weight tape placed in position 3) and B and underestimated by method C. Methods D and E accurately estimated BW of CAM horses. Correction factors are required to accurately estimate BW in this breed using methods B and C. Method A with the weight tape placed in position 2 is the most accurate for predicting BW, including pregnant female horses, and can, therefore, be considered the most suitable method for estimating BW of CAM horses.
摘要本研究的目的是评估以下六种体重估计方法在Campolina(CAM)马中的准确性:A-放置在动物胸部三个不同位置的重量带;B-Crevat和Quetelet公式;C——霍尔公式;D-Hintz和Griffiths的表;E-桑托斯表;以及F-Cintra公式。共有380匹CAM马根据性别、年龄等级和妊娠期进行了分离和评估。为了确定其准确性,使用R软件,通过配对t检验、平均预测误差(MPE)和决定系数(R2)比较在量表上测量的权重和六种方法的权重估计。方法F的预测能力在6-12个月年龄组中较低,因此该公式不适用。方法A(重量带放置在位置3)和方法B高估了BW与实际重量的比较,方法C低估了BW。方法D和E准确估计了CAM马的BW。使用方法B和C准确估计该品种的BW需要校正系数。将重量带放置在位置2的方法A是预测BW(包括怀孕母马)最准确的方法,因此可以被认为是估计CAM马BW的最合适方法。
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引用次数: 1
Milking practices on commercial Holstein-Friesian farms 荷斯泰因-弗里西亚商业农场的挤奶实践
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-09-15 DOI: 10.37496/rbz5020200280
L. Ózsvári, Dorottya Ivanyos
ABSTRACT The objective of this study was to survey and analyze milking practices of commercial Holstein-Friesian farms. A total of 43 Hungarian dairy farms with 31,430 cows was surveyed by using a questionnaire via personal interviews. Furthermore, seven in-depth, individual interviews were conducted with farm managers. In the statistical analysis, we used ANOVA models. The results showed that 57.8% of the milking parlors had a herringbone design, followed by parallel (20.0%) and rotary (17.8%) milking systems. For the interviewed farm managers, gentle (71.4%) and quick milking (57.1%) as well as herd size (57.1%) were the most important factors in determining the milking system design. In 62.8% of the farms, cows were milked twice a day, and the average milking time was 5.0 h with an average of 3.1 laborers. The average daily milking time per cow was 15 min and the average daily walking time per cow to the milking parlor and back was 24 min. Furthermore, 85.4% of the farms used traditional elastic milk liners, whereas 14.6% used silicone ones. In total, 57.1% of the interviewed farm managers said that the ideal teat liner should fit the teats correctly and provide gentle milking. Prior to milking, 65.1% of the farms used disinfectant dip and 11.6% used a disinfectant wash, whereas 23.3% still washed the udder with water. The udder was wiped with paper towels in 73.8% of the herds and with cloth in 26.2% of the herds. Forestripping was performed in all herds: 51.2% onto the floor, 46.5% into a cup, and in 2.3% into a paper towel. Further, 85.7% of the interviewed farm managers considered the use of a cup to be the ideal method, but 57.1% deemed a dark-colored piece of rubber/flooring to be similarly acceptable. Cows with mastitis were milked separately in 91.9% of the farms.
摘要本研究的目的是调查和分析荷尔斯泰因-弗里森商业农场的挤奶实践。通过个人访谈,对43个匈牙利奶牛场(31430头奶牛)进行了问卷调查。此外,还对农场管理人员进行了七次深入的个人访谈。在统计分析中,我们使用了方差分析模型。结果显示,57.8%的挤奶厅采用人字形设计,其次是平行挤奶系统(20.0%)和旋转挤奶系统(17.8%)。对于受访的农场管理人员来说,温和挤奶(71.4%)、快速挤奶(57.1%)以及牛群规模(57.1%)是决定挤奶系统设计的最重要因素。62.8%的奶牛场每天挤奶两次,平均挤奶时间为5.0小时,平均3.1名工人。每头牛的平均每日挤奶时间为15分钟,每头牛往返挤奶厅的平均每日步行时间为24分钟。此外,85.4%的农场使用传统的弹性牛奶衬垫,而14.6%的农场使用硅胶衬垫。总的来说,57.1%的受访农场经理表示,理想的乳头衬垫应该正确贴合乳头,并提供温和的挤奶。挤奶前,65.1%的农场使用消毒剂蘸液,11.6%的农场使用消毒液,而23.3%的农场仍然用水清洗乳房。73.8%的牛群用纸巾擦拭乳房,26.2%的牛群用布擦拭乳房。所有鹿群都进行了森林砍伐:51.2%的鹿群倒在地板上,46.5%的鹿群放在杯子里,2.3%的鹿群则放在纸巾里。此外,85.7%的受访农场经理认为使用杯子是理想的方法,但57.1%的人认为深色橡胶/地板也是可以接受的。91.9%的奶牛场分别对患有乳腺炎的奶牛挤奶。
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引用次数: 3
Investigation of the strength of copper and zinc bonds with other constituents of ruminant feedstuffs 反刍动物饲料中铜和锌与其他成分结合强度的研究
IF 1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2021-09-15 DOI: 10.37496/rbz5020190227
F. Leonel, L. Moreira, D. Zanetti, R. S. Gomes, T. Engle
ABSTRACT The objective of the present study was to evaluate the effect of feed type, pH, and Cu and Zn concentration on Cu and Zn binding with different feedstuffs after in vitro incubation in water. For this purpose, six feedstuffs (wheat straw, grass hay, corn silage, dried distillers grains with solubles, ground corn, and soybean meal) were incubated in ultrapure water (pH≈6.4) with supplemental Cu concentrations equivalent to 0, 5, 10, 15, 20, 30, and 40 mg Cu/kg DM or [...]
摘要本研究的目的是评估饲料类型、pH值以及Cu和Zn浓度对体外培养后不同饲料中Cu和Zn结合的影响。为此,将六种饲料(小麦秸秆、干草、玉米青贮饲料、含可溶性干酒糟、磨碎的玉米和豆粕)在超纯水(pH≈6.4)中孵育,补充的Cu浓度相当于0、5、10、15、20、30和40 mg Cu/kg DM或[…]
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引用次数: 0
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Revista Brasileira De Zootecnia-Brazilian Journal of Animal Science
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