Luminescence最新文献

Prostate-specific antigen (PSA) serves as a critical biomarker for the early diagnosis and monitoring of prostate malignancies. In this work, we report the fabrication of a carboxy-functionalized porous organic polymer (POP) as a highly sensitive and selective fluorescent immunosensor for PSA detection. The POP was synthesized via a solvothermal Schiff base condensation, which bears free –COOH functionalities to facilitate covalent antibody immobilization through classical EDC/NHS coupling chemistry. The resulting POP–antibody bioconjugate exhibited a pronounced fluorescence quenching response upon PSA binding, attributed to static quenching via ground-state complex formation, as confirmed by steady-state and time-resolved photoluminescence studies. Under optimized conditions, the sensor achieved a limit of detection (LOD) of 14 pg/mL, demonstrating a wide linear dynamic range and minimal cross-reactivity with nontarget biomolecules. The high surface area and hierarchical porosity of the POP matrix contributed to enhanced probe–analyte interactions and signal amplification. Notably, the sensor retained excellent performance in complex matrices, with successful PSA quantification in artificial urine and blood samples, highlighting its potential for real-world clinical diagnostics. The synergistic integration of structural surface functionality and photostability in this POP-based platform underscores its promise for ultrasensitive, low-cost, and point-of-care biosensing of cancer biomarkers.

Cerebrolysin and donepezil are frequently co-administered for the management of Alzheimer disease. This research established innovative, efficient, and inexpensive spectrofluorimetric methods to quantify cerebrolysin and donepezil. Method I utilized first derivative synchronous fluorometry for the first time to simultaneously estimate CRB and donepezil (DNP) at 325 and 350 nm, respectively, with a Δλ of 40 nm. A rectilinear correlation between concentration and response was observed across 2.00–20.0 μg/mL and 0.03–1.00 μg/mL for CRB and DNP, respectively. The method demonstrated notable sensitivity, with quantitation and detection limits of 0.72 and 0.013 μg/mL, and 0.24 and 0.004 μg/mL, for CRB and DNP, respectively. Method II describes a spectrofluorometric method for measuring the native fluorescence of CRB in water at 277/345 nm across the range of 1.00–10.0 μg/mL with detection and quantitation limits of 0.69 and 0.23 μg/mL, respectively. These methodologies were effectively applied to various dosage forms with satisfactory percentage recoveries. They were further successfully applied to spiked human plasma samples, proving their applicability to therapeutic drug monitoring for inpatients. Additionally, the environmental impact of these methods was assessed using four different greenness criteria.

A polycarbazole−polythiophene (PCZ–PTh) copolymer was synthesized through chemical oxidative polymerization for the electrochemical sensing of tryptophan (TP). The synthesis approach was straightforward, eco-friendly, and cost-efficient. The copolymer was characterized comprehensively using various analytical techniques, confirming a well-defined semi-sheet-like morphology. When immobilized onto a glassy carbon electrode (GCE), the copolymer significantly enhanced TP oxidation, demonstrating high sensitivity with detection and quantification limits of 0.125 μM, and the LOQ was 0.41 μM at neutral pH (7). The PCZ–PTh-modified GCE exhibited remarkable catalytic efficiency and analytical utility. Additionally, the PCZ–PTh/GCE displayed excellent reproducibility and selectivity for TP detection, even in complex environments containing dopamine, caffeine, and various metal ions, making it highly suitable for precise TP quantification in biological samples. The composite exhibited an improved specific capacitance of 173 F/g at a current density of 1 A/g. The superior electrochemical properties of PCZ–PTh-based super capacitors highlight their viability as a robust nanomaterial for advanced applications, offering substantial promise for high-energy-density systems and advancements in energy storage technologies.

Ferric ion plays an important role in various physiological processes and is crucial to human health. Fluorescent sensing of ferric ions based on carbon dots is attracting intensive interests owing to the prominent merits of carbon dots such as easy preparation, high photostability, low toxicity, and high solubility in water. In this paper, one kind of nitrogen-doped carbon dots (CDs-AE) was reported, which exhibited strong fluorescence with emission peaked at 480 nm. Notably, the presence of ferric ions can efficiently quench the photoluminescence of CDs-AE, and the quenching efficiency was as high as 94%. Finally, the quenching mechanism behind was explored and the application of CDs-AE in ferric ion detection in real sample was studied.

Ion beam luminescence (IBL) measurements were conducted on natural tridymite samples from Lanzarote (Canary Island) to evaluate the spectral characteristics of this SiO₂ polymorph under varying thermal and cumulative irradiation conditions. Temperature-dependent IBL spectra were recorded between 80 and 330 K (from −196.15°C to 56.85°C with an interval of 10°C), revealing prominent emissions in the UV–visible range. Additionally, the response to 15 successive proton irradiations at room temperature was assessed to investigate dose-dependent stability: (i) The emissions at ~343, ~408, and ~493 nm exhibited thermally activated non radiative quenching and (ii) the peaks at ~244, ~644, and ~880 nm remained largely resistant to cumulative ion exposure. Results were compared with limited IBL data on quartz systems in the literature. The observed stability of emission features suggest potential applications in radiation sensing and defect structure studies.

To further investigate the optical properties of pyridyl-isoindoline-1-one derivatives and expand their practical applications, two novel difluoroboron dyes, a pyrenyl-substituted compound Isoin-py BF₂ and an anthryl-substituted compound Isoin-en BF₂, were conveniently synthesized in three steps. Both compounds exhibited aggregation-induced emission in DMSO/water mixtures and solid-state luminescence. Furthermore, their solutions could be used as developers for latent fingerprint imaging on three types of substrates. Interestingly, the imaging mechanism originates from a specific response of Isoin-py BF₂ to oleic acid and of Isoin-en BF₂ to lactic acid, both secreted in sweat. The developer based on Isoin-en BF₂ could clearly reveal all three levels of fingerprint features, outperforming Isoin-py BF₂ in detail resolution. Additionally, Isoin-py BF₂ exhibited reversible mechanofluorochromic behavior due to a phase transition between crystalline states, enabling its application ink-free writing. In contrast, Isoin-en BF₂ showed no mechanofluorochromism due to its strong crystallinity. This work provides a reference for the molecular design and development of boron-pyridyl-isoindoline-1-one-based dyes containing bulky π-conjugated substituents.

Etching is a well-established method to create particles with nano-level precision. Herein, we have synthesized vanadium carbide (V₂C) MXene fluorescent nanoparticles (FNPs) for the determination of rabeprazole sodium (R-Na) in drug samples. The prepared V₂C MXene FNPs show blue emission under 365 nm of UV light with a good quantum yield of 9.70%. The synthesized V₂C MXene FNPs showed the highest fluorescent intensity up on excitation at 350 nm, giving a maximum emission wavelength of 428 nm. Fascinatingly, the fluorescence intensity of V₂C MXene FNPs shows remarkable quenching upon the addition of R-Na over a range of 0.1–5 μM with a detection limit of 0.09 μM. The designed V₂C MXene FNP–based probe was successfully applied to the assay of R-Na in its pharmaceutical samples and biofluids. This work is also assessed in terms of its greenness and blueness using metrics like the Complex Green Analytical Procedure Index (GAPI) and the Blue Applicability Grade Index (BAGI) tools. Bringing together, V₂C MXene FNPs could be admitted as a promising fluorescent probe for assaying R-Na in drug samples with justifiable accuracy and precision.

High-brightness and thermally stable chartreuse-emitting phosphors are highly desirable for high-quality phosphor-converted white light–emitting diodes (pc-LEDs). In this article, a new chartreuse phosphor, Lu_(1-x)Mg₂Al₃Si₂O₁₂:xCe³⁺ (LMASO:xCe³⁺), was synthesized via a high-temperature solid-state reaction. X-ray diffraction and Rietveld refinement reveal that LMASO phosphors crystallize in the cubic system (Ia$��\overline{3}�$$��\stackrel{-}{3}�$d). The optimal doping concentration of Ce³⁺ in LMASO is 10 mol%, which is higher than that of other Ce³⁺-doped garnet phosphors. Its broad excitation band peaking at 429 nm matches well with the emission from commercial blue LED chips. Under 429 nm excitation, the LMASO:Ce³⁺ phosphor exhibits a wide asymmetric emission band ranging from 450 to 650 nm. The high activation energy (0.334 eV) indicates a good thermal quenching resistance. A white LED device was fabricated by combining a 450nm blue LED chip with the LMASO:0.10Ce³⁺ chartreuse phosphor and a commercial CaAlSiN₃:Eu²⁺ red phosphor. This device emits bright warm-white light with excellent CIE color coordinates (0.3978, 0.3959), a low correlated color temperature (CCT = 3500 K) and a high color rendering index (Ra = 95.7). The results demonstrate that LMASO:Ce³⁺ phosphor is a promising candidate for pc-LEDs.

A novel, sensitive, and reliable spectrofluorimetric method for the determination of a unique antinarcoleptic drug armodafinil in human plasma and dosage forms was developed for the first time. The proposed method is based on the formation of a highly yellow fluorescent derivative through the reaction of the studied drug with acetylacetone and formaldehyde in an acetate buffer (pH 4.5), yielding a strong emission at 485 nm following excitation at 415 nm. The experimental variables that affected the one-pot synthesis were investigated and optimized. The calibration curve demonstrated a linear relationship over a concentration range of 0.2–2.0 μg/mL, with low detection and quantitation limits of 0.03 and 0.09 μg/mL, respectively. The designed method was validated following the ICH guidelines, permitting precise analysis of the studied drug in its tablets and human plasma samples with high recovery. Furthermore, the environmental sustainability of the suggested method was assessed using contemporary tools for greenness, whiteness, and blueness evaluation. The results demonstrated that the method possesses excellent eco-friendly characteristics, highlighting its strong alignment with the principles of green analytical chemistry.

A novel spectrofluorometric method that leverages ion-pair complexation with propidium iodide to achieve indirect fluorescence enhancement for the sensitive measurement of zuranolone is introduced. In acidic media, protonated propidium iodide interacts selectively with negatively charged zuranolone, forming a stable ion-pair complex. This interaction induces conformational changes in propidium iodide, markedly increasing its fluorescence and enabling accurate detection of zuranolone. The method achieved quantification within the range of 10–300 ng/mL, with limits of detection and quantification of 2.50 and 7.50 ng/mL, respectively. Validation according to International Conference on Harmonization guidelines confirmed its linearity, precision, accuracy, and selectivity. Application to Zurzuvae capsules demonstrated excellent accuracy and reliable analytical performance. Furthermore, high Analytical GREEnness and favorable Green Analytical Procedure Index scores highlighted the method's environmental sustainability, positioning it as a robust, eco-friendly tool for routine analysis.