Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2810
D. Szteren, D. Lercari
A notable diversity of marine mammals inhabits the Atlantic and Pacific waters of South America. For decades, South American countries have been producing scientific research focused on these species. However, still, there is no systematic assessment of the magnitude and main subjects on which this research has been focused. This study analyzes the trends and patterns in scientific research on marine mammals in South America, evaluating a pool of bibliometric indicators and mapping collaborative relationships among countries, authors, and research areas. Academic documents were retrieved from two bibliographic databases: SCOPUS and SciELO, from 1990 to 2020. Results showed a gradual increase in publications along the three study decades. Brazil played a central role in the number of publications in both databases, followed by Argentina and Chile. The South American publications on marine mammals were centralized in a small number of journals, and few authors were responsible for a large proportion of contributions. The authors showed a moderate level of collaboration, mainly reflecting stronger links among neighbor countries, including co-authorships with North American and European countries. The most frequent keywords reflected three clusters centered in taxonomic groups (Cetacea, Odontoceti, and Pinnipedia) and two centered in research subjects (pollution and phylogenetics). The scope of the contributions differed among collections. Nevertheless, both databases were complementary and contributed to show marine mammals' research in South America.
{"title":"Marine mammal research in South America: 30 years of publication efforts and collaborative networks","authors":"D. Szteren, D. Lercari","doi":"10.3856/vol50-issue2-fulltext-2810","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2810","url":null,"abstract":"A notable diversity of marine mammals inhabits the Atlantic and Pacific waters of South America. For decades, South American countries have been producing scientific research focused on these species. However, still, there is no systematic assessment of the magnitude and main subjects on which this research has been focused. This study analyzes the trends and patterns in scientific research on marine mammals in South America, evaluating a pool of bibliometric indicators and mapping collaborative relationships among countries, authors, and research areas. Academic documents were retrieved from two bibliographic databases: SCOPUS and SciELO, from 1990 to 2020. Results showed a gradual increase in publications along the three study decades. Brazil played a central role in the number of publications in both databases, followed by Argentina and Chile. The South American publications on marine mammals were centralized in a small number of journals, and few authors were responsible for a large proportion of contributions. The authors showed a moderate level of collaboration, mainly reflecting stronger links among neighbor countries, including co-authorships with North American and European countries. The most frequent keywords reflected three clusters centered in taxonomic groups (Cetacea, Odontoceti, and Pinnipedia) and two centered in research subjects (pollution and phylogenetics). The scope of the contributions differed among collections. Nevertheless, both databases were complementary and contributed to show marine mammals' research in South America.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47367477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2795
Y. Méndez-Martínez, Magaly M. Puente-Mendoza, Yenny G. Torres-Navarrete, Ronald J. Zamora-Zambrano, Aroldo Botello León, J. L. Ramírez
A comparative study of serum biochemical and hematological parameters from Andinoacara rivulatus and Ichthyoelephas humeralis in Los Ríos Province, Ecuador, was carried out. Two of the native species that are the most commercialized in Quevedo, Mocache and Fumisa, were identified; 60 specimens were captured in each area (180 total). A factorial design (3×2) was used: three habitat zones (Fumisa, Quevedo, and Mocache) and two species (A. rivulatus and I. humeralis). Sex, weight, length, serum biochemistry (total protein, albumin, globulin, albumin/globulin ratio, aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium, phosphorus, sodium, magnesium, and potassium), hematological parameters (hemoglobin, hematocrits, erythrocytes, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total leukocytes, granulocytes leukocytes, and agranulocytes leukocytes) in fishes were determined. There was zone-species interaction for all indicators except MCH and MCHC (P < 0.05). For total proteins, albumins and ALT were for I. humeralis in Fumisa and Mocache zones (2.81, 1.57, and 326.67 g dL-1, respectively). In comparison, A. rivulatus was higher in Fumisa and Quevedo in globulins and the albumins/globulins ratio with 2.28 g dL-1 and 2.14, respectively. Electrolytes were found in low concentrations in the two species. For the hematological parameters, all presented significant differences (P < 0.05) except MCH and MCHC. For I. humeralis, its highest values were presented in hemoglobin with 13.37 g dL-1 in Fumisa, for MCV and total leukocytes (93.11 fL-1 and 8.03×103 μL-1) respectively, in the Mocache area. This study will provide essential tools in monitoring the health status of these fish species in particular and fish species in general.
对厄瓜多尔Los Ríos省两种鱼的血清生化和血液学指标进行了比较研究。在克维多发现了两种商业化程度最高的本地物种Mocache和Fumisa;每个区域共捕获标本60只(共180只)。采用因子设计(3×2): 3个生境区(Fumisa、Quevedo和Mocache)和2个物种(A. rivulatus和I. humeralis)。性别、体重、身高、血清生化(总蛋白、白蛋白、球蛋白、白蛋白/球蛋白比、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、钙、磷、钠、镁、钾)、血液学参数(血红蛋白、红细胞、平均红细胞体积(MCV)、平均红细胞血红蛋白(MCH)、平均红细胞血红蛋白浓度(MCHC)、总白细胞、粒细胞、白细胞、测定鱼体内的粒细胞(白细胞)。除MCH和MCHC外，其余指标均存在区种交互作用(P < 0.05)。总蛋白方面，肱骨棘在Fumisa区和Mocache区白蛋白和ALT分别为2.81、1.57和326.67 g dL-1。相比之下，富米沙和克维多地区的河纹田鼠的球蛋白和白蛋白/球蛋白比值较高，分别为2.28 g dL-1和2.14 g dL-1。在这两个物种中发现了低浓度的电解质。血液学指标除MCH、MCHC外，其余指标差异均有统计学意义(P < 0.05)。在Mocache地区，肱骨棘球蚴的血红蛋白含量最高，为13.37 g dL-1, MCV和总白细胞含量分别为93.11 μL-1和8.03×103 μL-1。这项研究将为监测这些鱼类的健康状况和一般鱼类的健康状况提供重要的工具。
{"title":"Comparative study of serum biochemical and hematological parameters of Andinoacara rivulatus and Ichthyoelephas humeralis in Los Ríos Province, Ecuador","authors":"Y. Méndez-Martínez, Magaly M. Puente-Mendoza, Yenny G. Torres-Navarrete, Ronald J. Zamora-Zambrano, Aroldo Botello León, J. L. Ramírez","doi":"10.3856/vol50-issue2-fulltext-2795","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2795","url":null,"abstract":"A comparative study of serum biochemical and hematological parameters from Andinoacara rivulatus and Ichthyoelephas humeralis in Los Ríos Province, Ecuador, was carried out. Two of the native species that are the most commercialized in Quevedo, Mocache and Fumisa, were identified; 60 specimens were captured in each area (180 total). A factorial design (3×2) was used: three habitat zones (Fumisa, Quevedo, and Mocache) and two species (A. rivulatus and I. humeralis). Sex, weight, length, serum biochemistry (total protein, albumin, globulin, albumin/globulin ratio, aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium, phosphorus, sodium, magnesium, and potassium), hematological parameters (hemoglobin, hematocrits, erythrocytes, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total leukocytes, granulocytes leukocytes, and agranulocytes leukocytes) in fishes were determined. There was zone-species interaction for all indicators except MCH and MCHC (P < 0.05). For total proteins, albumins and ALT were for I. humeralis in Fumisa and Mocache zones (2.81, 1.57, and 326.67 g dL-1, respectively). In comparison, A. rivulatus was higher in Fumisa and Quevedo in globulins and the albumins/globulins ratio with 2.28 g dL-1 and 2.14, respectively. Electrolytes were found in low concentrations in the two species. For the hematological parameters, all presented significant differences (P < 0.05) except MCH and MCHC. For I. humeralis, its highest values were presented in hemoglobin with 13.37 g dL-1 in Fumisa, for MCV and total leukocytes (93.11 fL-1 and 8.03×103 μL-1) respectively, in the Mocache area. This study will provide essential tools in monitoring the health status of these fish species in particular and fish species in general.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48193356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2815
Manuel Sacristán de Alva, Elsa Noreña Barroso, Edlin Guerra Castro, Iveth Gabriela Palomino Albarrán, Álvaro Barreto, G. Gaxiola
Microalgae can accumulate lipids during the stationary growth phase, but little information is available about fatty acid profile changes during this phase to determine the best harvesting time in respect to lipid content. In this study, Dunaliella sp. was cultured in F/2 medium at three different photon flux densities (380, 226 and 8.2 μmol photon m-2 s-1) and three different glycerol concentrations (0, 10 and 20 g L-1). Samples were taken during the stationary phase to assess lipid content and fatty acid profile variations. Microalgal biomass production was higher at 380 and 226 μmol photon m-2 s-1 than at 8.2 μmol photon m-2 s-1 in accord to light limitation. The maximum lipid content (345.78 mg g-1) was achieved at 8.2 μmol photon m-2 s-1 and 20 g L-1 glycerol at day 12, similar to that achieved at day 9 (334.16 mg g-1). The maximum polyunsaturated fatty acid amount (65.30 μg mg-1) was achieved at day 7 of culture without glycerol addition, decreasing in proportion over time. So, the best conditions and harvesting time in respect to fatty acid quality would be at 380 μmol photon m-2 s-1 without glycerol addition and after 7 days of culture.
{"title":"Fatty acid profile and productivity variation during the growth of Dunaliella sp. under different photon flux densities and glycerol concentrations","authors":"Manuel Sacristán de Alva, Elsa Noreña Barroso, Edlin Guerra Castro, Iveth Gabriela Palomino Albarrán, Álvaro Barreto, G. Gaxiola","doi":"10.3856/vol50-issue2-fulltext-2815","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2815","url":null,"abstract":"Microalgae can accumulate lipids during the stationary growth phase, but little information is available about fatty acid profile changes during this phase to determine the best harvesting time in respect to lipid content. In this study, Dunaliella sp. was cultured in F/2 medium at three different photon flux densities (380, 226 and 8.2 μmol photon m-2 s-1) and three different glycerol concentrations (0, 10 and 20 g L-1). Samples were taken during the stationary phase to assess lipid content and fatty acid profile variations. Microalgal biomass production was higher at 380 and 226 μmol photon m-2 s-1 than at 8.2 μmol photon m-2 s-1 in accord to light limitation. The maximum lipid content (345.78 mg g-1) was achieved at 8.2 μmol photon m-2 s-1 and 20 g L-1 glycerol at day 12, similar to that achieved at day 9 (334.16 mg g-1). The maximum polyunsaturated fatty acid amount (65.30 μg mg-1) was achieved at day 7 of culture without glycerol addition, decreasing in proportion over time. So, the best conditions and harvesting time in respect to fatty acid quality would be at 380 μmol photon m-2 s-1 without glycerol addition and after 7 days of culture.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47091507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2715
Rafael Fernández de Alaiza, Ubiratã De A.T. da Silva, Silvio Peixoto, C. Craveiro, C. H. Ferreira, E. Ballester
The objective of this study was to identify morphological characteristics of different populations of Penaeus schmitti Burkenroad, 1936 to contribute to its biology and conservation. In order to compare geographically isolated populations, between 2014 and 2016, selected biological variables were analyzed in six populations dispersed along the Brazilian coast and one from the Cuban coast. Specimens with eight upper rostral teeth predominated. The ordination of shrimp measurements by non-metric multidimensional scaling showed the greatest dispersion on data from Cuba, Rio Grande do Norte, and Espirito Santo, which are also the localities that presented the highest coefficient of variation. On the other hand, the values corresponding to Sergipe, Rio de Janeiro, Paraná, and Santa Catarina showed a greater similarity or clustering of the data of each location. Tail weight was estimated using length and depth of the sixth abdominal segment. The populations of Santa Catarina, Brazil (28°S), had the longest and heavier abdomens proportionally among all the compared populations, while the organisms of Manzanillo, Cuba (20°N), had proportionally shorter abdomens and the narrowest cephalothorax. The regression equation indicated a positive allometric growth for P. schmitti, and the largest total length reported for genus Penaeus in the natural environment corresponded to a P. schmitti specimen. This information could be useful in future taxonomic studies to correctly identify the species and its morphological variability. Color differences were also observed, with white ventral spots in the sternite XIV of some females from Brazilian populations, which was not previously reported in the literature. Extending these studies is recommended to contribute to the conservation and culture of P. schmitti.
本研究的目的是确定schmitti Burkenroad对虾（1936）不同种群的形态特征，为其生物学和保护做出贡献。为了比较地理上孤立的种群，在2014年至2016年间，对分布在巴西海岸的六个种群和一个来自古巴海岸的种群的选定生物变量进行了分析。样本中有8颗上颌齿占主导地位。通过非度量多维尺度对虾的测量排序显示，古巴、北里奥格兰德州和圣埃斯皮里图州的数据差异最大，这些地区也是变异系数最高的地区。另一方面，Sergipe、Rio de Janeiro、Paraná和Santa Catarina对应的值显示出每个位置的数据具有更大的相似性或聚类性。根据第六腹部的长度和深度估算尾部重量。在所有比较种群中，巴西圣卡塔琳娜（28°S）种群的腹部按比例最长、最重，而古巴曼萨尼约（20°N）的生物则按比例较短，头胸最窄。回归方程表明，schmitti对虾正异速生长，据报道，在自然环境中，Penaeus属的最大总长度对应于schmitti龙虾标本。这些信息可能对未来的分类学研究有用，以正确识别该物种及其形态变异性。还观察到了颜色差异，巴西种群中一些雌性的胸骨XIV上有白色腹侧斑点，这在以前的文献中没有报道。建议扩大这些研究范围，以促进施密蒂P.schmitti的保护和培养。
{"title":"Morphological variations of southern white shrimp Penaeus schmitti (Burkenroad, 1936) (Crustacea: Dendrobranchiata: Penaeidae) in natural populations of Cuba and Brazil","authors":"Rafael Fernández de Alaiza, Ubiratã De A.T. da Silva, Silvio Peixoto, C. Craveiro, C. H. Ferreira, E. Ballester","doi":"10.3856/vol50-issue2-fulltext-2715","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2715","url":null,"abstract":"The objective of this study was to identify morphological characteristics of different populations of Penaeus schmitti Burkenroad, 1936 to contribute to its biology and conservation. In order to compare geographically isolated populations, between 2014 and 2016, selected biological variables were analyzed in six populations dispersed along the Brazilian coast and one from the Cuban coast. Specimens with eight upper rostral teeth predominated. The ordination of shrimp measurements by non-metric multidimensional scaling showed the greatest dispersion on data from Cuba, Rio Grande do Norte, and Espirito Santo, which are also the localities that presented the highest coefficient of variation. On the other hand, the values corresponding to Sergipe, Rio de Janeiro, Paraná, and Santa Catarina showed a greater similarity or clustering of the data of each location. Tail weight was estimated using length and depth of the sixth abdominal segment. The populations of Santa Catarina, Brazil (28°S), had the longest and heavier abdomens proportionally among all the compared populations, while the organisms of Manzanillo, Cuba (20°N), had proportionally shorter abdomens and the narrowest cephalothorax. The regression equation indicated a positive allometric growth for P. schmitti, and the largest total length reported for genus Penaeus in the natural environment corresponded to a P. schmitti specimen. This information could be useful in future taxonomic studies to correctly identify the species and its morphological variability. Color differences were also observed, with white ventral spots in the sternite XIV of some females from Brazilian populations, which was not previously reported in the literature. Extending these studies is recommended to contribute to the conservation and culture of P. schmitti.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48811860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2847
R. Domínguez-Maldonado, C. A. Villegas-Sánchez, M. Ruiz-Zárate, C. González-Salas, Oscar de Jesús Rosado-Nic
The populations of Acropora palmata have decreased over the last four decades, and although there are several studies on their genetic diversity in the Caribbean, no studies have been published about the reefs from the southern Gulf of Mexico. This research aimed to determine, using five microsatellite markers, the genetic variation of three A. palmata populations in the Mexican Atlantic located in the southwest Gulf of Mexico, in the Campeche Bank, and in the Caribbean. The mean of genetic richness (Ng/N) in the studied reefs was 0.583; the lowest estimated value corresponded to the Campeche Bank reef. A low genetic diversity was registered in the studied reefs (reef mean He = 0.315 ± 0.052). The significant genetic structure observed among studied populations could be related to ocean currents from the region and specific biological characteristics, mainly, short larvae phase, high mortality, and high rate of self-recruitment. These results may be particularly useful for designing management strategies, considering the lack of studies of this type in the region.
{"title":"Genetic differences of Acropora palmata populations of the Mexican Atlantic","authors":"R. Domínguez-Maldonado, C. A. Villegas-Sánchez, M. Ruiz-Zárate, C. González-Salas, Oscar de Jesús Rosado-Nic","doi":"10.3856/vol50-issue2-fulltext-2847","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2847","url":null,"abstract":"The populations of Acropora palmata have decreased over the last four decades, and although there are several studies on their genetic diversity in the Caribbean, no studies have been published about the reefs from the southern Gulf of Mexico. This research aimed to determine, using five microsatellite markers, the genetic variation of three A. palmata populations in the Mexican Atlantic located in the southwest Gulf of Mexico, in the Campeche Bank, and in the Caribbean. The mean of genetic richness (Ng/N) in the studied reefs was 0.583; the lowest estimated value corresponded to the Campeche Bank reef. A low genetic diversity was registered in the studied reefs (reef mean He = 0.315 ± 0.052). The significant genetic structure observed among studied populations could be related to ocean currents from the region and specific biological characteristics, mainly, short larvae phase, high mortality, and high rate of self-recruitment. These results may be particularly useful for designing management strategies, considering the lack of studies of this type in the region.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46378119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2824
Marcelo U. García-Guerrero, Noe Avilés-Espinoza, Glenda J. Lizarraga-Sanchez, G. Herrera-Rodríguez, David Váldez-Martínez, Pedro Hernández-Sandoval
Macrobrachium americanum is a large prawn living in rivers and coastal water bodies of tropical and subtropical ecosystems of the Pacific side of the Americas. Water temperature and salinity are the main physicochemical variables determining its distribution. Temperature also influences its metabolic rate, which determines growth rate and reproduction. Because of this, preferred temperature, critical thermal maximum (CTMax), and O2 consumption rates at four temperatures (20, 24, 28, and 32°C) were tested from wild prawns in laboratory assays. Those temperatures correspond to the annual temperature fluctuations at the sampling site. The final preferred temperature of this species is close to 26.4°C. The acclimatization temperature has an almost linear effect on the CTMax (R2 = 0.987, P < 0.05), and the oxygen consumption routine rates increased as the acclimation temperature increased from 20 to 32°C.
美洲巨臂虾是一种生活在美洲太平洋一侧热带和亚热带生态系统的河流和沿海水体中的大型对虾。水温和盐度是决定其分布的主要物化变量。温度也影响其代谢率，代谢率决定生长速度和繁殖。因此，对野生对虾在4种温度(20、24、28和32℃)下的首选温度、临界热最大值(CTMax)和氧气消耗率进行了实验室检测。这些温度与采样地点的年温度波动相对应。该物种的最终偏好温度接近26.4°C。驯化温度对CTMax的影响呈近似线性关系(R2 = 0.987, P < 0.05)，在驯化温度为20 ~ 32℃的范围内，随驯化温度的升高，常规耗氧量增加。
{"title":"Maximum critical temperature and oxygen consumption during thermoregulation in Macrobrachium americanum (Bate, 1868) adult prawns","authors":"Marcelo U. García-Guerrero, Noe Avilés-Espinoza, Glenda J. Lizarraga-Sanchez, G. Herrera-Rodríguez, David Váldez-Martínez, Pedro Hernández-Sandoval","doi":"10.3856/vol50-issue2-fulltext-2824","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2824","url":null,"abstract":"Macrobrachium americanum is a large prawn living in rivers and coastal water bodies of tropical and subtropical ecosystems of the Pacific side of the Americas. Water temperature and salinity are the main physicochemical variables determining its distribution. Temperature also influences its metabolic rate, which determines growth rate and reproduction. Because of this, preferred temperature, critical thermal maximum (CTMax), and O2 consumption rates at four temperatures (20, 24, 28, and 32°C) were tested from wild prawns in laboratory assays. Those temperatures correspond to the annual temperature fluctuations at the sampling site. The final preferred temperature of this species is close to 26.4°C. The acclimatization temperature has an almost linear effect on the CTMax (R2 = 0.987, P < 0.05), and the oxygen consumption routine rates increased as the acclimation temperature increased from 20 to 32°C.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41647983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2839
Sarah Afrin Píndaro Alvarez-Ruiz, A. Luna-González, Ruth Escamilla-Montes, A. Fierro-Coronado, Genaro Diarte-Plata, C. García-Gutiérrez, Viridiana Peraza-Gómez
The effect of Vibrio parahaemolyticus IPNGS16 on the bacterial profile of the gut of Penaeus vannamei was assessed by 16S metagenomic analysis. The V3 hypervariable region of the bacterial 16S rDNA was amplified by PCR. Sequencing reads were generated using the 2×150 (300 cycles) for the base-read length chemistry of the Illumina MiniSeq platform. The web-based Shaman and MicrobiomeAnalyst platforms were used to analyze the sequences. The phyla Proteobacteria, Bacteroidetes, and the genera Vibrio, Ruegeria, Nautella, and Pseudoalteromonas were found among the most abundant taxonomic ranks in control, diseased, and healthy shrimp. Alpha and beta indices showed significant differences between shrimp survival in the control condition and dying shrimp (lower diversity). Metabolism (carbohydrate and amino acid metabolism-related genes and, to a lesser extent, energy, lipid, and cofactors and vitamin metabolism-related genes) of dying and surviving shrimp was affected by Vibrio infection. The top metabolic functions (cell cycle, glycine, serine, threonine, cysteine, methionine, purine, pyrimidine, pyruvate, and quorum sensing) in dying and surviving shrimp were affected by Vibrio, especially quorum sensing. The interaction network analysis showed fewer interactions in dying shrimp than control and surviving shrimp. Proteobacteria, Bacteroidetes, Vibrio, and Ruegeria predominated in all samples, and Vibrio changed bacterial diversity and metabolism in the intestine of P. vannamei. Ruegeria and Pseudoalteromonas showed negative interactions with Vibrio, suggesting their use as probiotics. This study sheds light on the Vibrio infection in the gut microbiota of shrimp.
{"title":"Gut bacterial profile associated with healthy and diseased (AHPND) shrimp Penaeus vannamei","authors":"Sarah Afrin Píndaro Alvarez-Ruiz, A. Luna-González, Ruth Escamilla-Montes, A. Fierro-Coronado, Genaro Diarte-Plata, C. García-Gutiérrez, Viridiana Peraza-Gómez","doi":"10.3856/vol50-issue2-fulltext-2839","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2839","url":null,"abstract":"The effect of Vibrio parahaemolyticus IPNGS16 on the bacterial profile of the gut of Penaeus vannamei was assessed by 16S metagenomic analysis. The V3 hypervariable region of the bacterial 16S rDNA was amplified by PCR. Sequencing reads were generated using the 2×150 (300 cycles) for the base-read length chemistry of the Illumina MiniSeq platform. The web-based Shaman and MicrobiomeAnalyst platforms were used to analyze the sequences. The phyla Proteobacteria, Bacteroidetes, and the genera Vibrio, Ruegeria, Nautella, and Pseudoalteromonas were found among the most abundant taxonomic ranks in control, diseased, and healthy shrimp. Alpha and beta indices showed significant differences between shrimp survival in the control condition and dying shrimp (lower diversity). Metabolism (carbohydrate and amino acid metabolism-related genes and, to a lesser extent, energy, lipid, and cofactors and vitamin metabolism-related genes) of dying and surviving shrimp was affected by Vibrio infection. The top metabolic functions (cell cycle, glycine, serine, threonine, cysteine, methionine, purine, pyrimidine, pyruvate, and quorum sensing) in dying and surviving shrimp were affected by Vibrio, especially quorum sensing. The interaction network analysis showed fewer interactions in dying shrimp than control and surviving shrimp. Proteobacteria, Bacteroidetes, Vibrio, and Ruegeria predominated in all samples, and Vibrio changed bacterial diversity and metabolism in the intestine of P. vannamei. Ruegeria and Pseudoalteromonas showed negative interactions with Vibrio, suggesting their use as probiotics. This study sheds light on the Vibrio infection in the gut microbiota of shrimp.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44398100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2814
Á. Campa-Córdova, C. Angulo, M. Zarain-Herzberg, R. Pacheco-Marges, F. Ascencio, M. Guzmán-Murillo, Ana C. Sánchez-Ortiz, A. Hernández‐Llamas
The stressing effect of stocking density and rearing time was assessed on shrimp Penaeus vannamei reared intensively in floating cages. Juvenile shrimp were stocked in 9 m2 cages at 200, 250, and 300 ind m-2 for a 25-day grow-out period. Total soluble protein concentration, superoxide dismutase (SOD), and catalase (CAT) activities were used as stress indicators in shrimp muscle, hepatopancreas, and hemocytes. Two-way ANOVA showed that protein content in hepatopancreas significantly decreased as stocking rate increased (from 3.81 to 2.26 mg mL-1). Density and rearing time interacted significantly to determine SOD activity in muscle and hemocytes where the maximum activity occurred at the densest rate by the end of the study (10.21 and 122.41 U mg-1); CAT activity in hepatopancreas was significantly lower when the highest density (4.1 U mg-1) was used. Final shrimp weight (5.28-5.49 g), survival (90.2-91.9%), feed conversion ratio (0.75-0.78), specific (0.058-0.063), and absolute (1.17-1.30 g week-1) growth rates were not significantly affected by density, although yields varied significantly (0.99-1.49 kg m-2). To conclude, despite the stocking rate causing a stressing effect on shrimp, its antioxidant enzymatic activity prevented such development from negatively affecting shrimp growth, survival, feed conversion ratio, and production.
以浮动网箱集养的凡纳滨对虾为试验对象，研究了放养密度和饲养时间的胁迫效应。稚虾分别在200、250和300 μ m-2的9 m2笼中放养，生长期为25 d。以虾肌肉、肝胰腺和血细胞的总可溶性蛋白浓度、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性为应激指标。双因素方差分析显示，随着放养率的增加，肝胰脏蛋白质含量显著降低(从3.81 mg mL-1降至2.26 mg mL-1)。密度和饲养时间显著相互作用，决定了肌肉和血细胞中SOD活性，其中在研究结束时密度率最高(10.21和122.41 U mg-1);在最高浓度(4.1 U mg-1)时，肝胰脏CAT活性显著降低。密度对末重(5.28 ~ 5.49 g)、存活率(90.2 ~ 91.9%)、饲料系数(0.75 ~ 0.78)、比生长率(0.058 ~ 0.063)和绝对生长率(1.17 ~ 1.30 g周-1)影响不显著，但产量差异显著(0.99 ~ 1.49 kg m-2)。综上所述，尽管放养率对对虾产生应激效应，但其抗氧化酶活性阻止了应激效应对对虾生长、存活、饲料系数和产量的负面影响。
{"title":"Stressing stocking density and rearing time effect on whiteleg shrimp (Penaeus vannamei) reared intensively in floating cages","authors":"Á. Campa-Córdova, C. Angulo, M. Zarain-Herzberg, R. Pacheco-Marges, F. Ascencio, M. Guzmán-Murillo, Ana C. Sánchez-Ortiz, A. Hernández‐Llamas","doi":"10.3856/vol50-issue2-fulltext-2814","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2814","url":null,"abstract":"The stressing effect of stocking density and rearing time was assessed on shrimp Penaeus vannamei reared intensively in floating cages. Juvenile shrimp were stocked in 9 m2 cages at 200, 250, and 300 ind m-2 for a 25-day grow-out period. Total soluble protein concentration, superoxide dismutase (SOD), and catalase (CAT) activities were used as stress indicators in shrimp muscle, hepatopancreas, and hemocytes. Two-way ANOVA showed that protein content in hepatopancreas significantly decreased as stocking rate increased (from 3.81 to 2.26 mg mL-1). Density and rearing time interacted significantly to determine SOD activity in muscle and hemocytes where the maximum activity occurred at the densest rate by the end of the study (10.21 and 122.41 U mg-1); CAT activity in hepatopancreas was significantly lower when the highest density (4.1 U mg-1) was used. Final shrimp weight (5.28-5.49 g), survival (90.2-91.9%), feed conversion ratio (0.75-0.78), specific (0.058-0.063), and absolute (1.17-1.30 g week-1) growth rates were not significantly affected by density, although yields varied significantly (0.99-1.49 kg m-2). To conclude, despite the stocking rate causing a stressing effect on shrimp, its antioxidant enzymatic activity prevented such development from negatively affecting shrimp growth, survival, feed conversion ratio, and production.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47561353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2777
Missileny Xavier, W. Wasielesky Júnior, Bárbara Hostins, E. Bequé, Dariano Krummenauer
Biofloc technology is a culture system that intensively uses microbial communities. Most bacteria can form aggregates around organic or inorganic surfaces. The high adsorption capacity, surface area, and flocculant properties of clay minerals make these compounds good candidates to stimulate biofloc formation, increase bacterial biomass, and improve nitrification. The objective of this study was to evaluate the addition of clay minerals to biofloc formation and its influence on the microbial community, nitrification, and zootechnical performance of Penaeus vannamei cultivated in a biofloc technology system. Experiment 1 involved the following treatments: control (without adding clay minerals), 10, 50, and 100 mg L-1, applied daily in the water. The treatments in experiment 2 were: control without product and inoculum, control without product and with inoculum, 50 mg L-1 clay mineral without inoculum and 50 mg L-1 clay mineral with inoculum. Both experiments with a density of 400 ind m-3. NO2 levels, total suspended solids, turbidity, differed significantly (P < 0.05) between treatments in experiment 1 and zootechnical performance parameters, where higher flocculant concentrations negatively affected these indices. In experiment 2, statistical differences were verified for NH3 and NO2 concentrations and the proximate composition of the bioflocs (P < 0.05), the addition of the inoculum of bioflocs influenced these results. In summary, the addition of clay minerals influenced biofloc formation, proximal composition, and the zootechnical performance of the cultivated shrimp but did not affect the abundance of microorganisms and nitrification.
{"title":"The use of a flocculant additive and its effect on biofloc formation, nitrification, and zootechnical performance during the culture of Pacific white shrimp Penaeus vannamei (Boone, 1931) in a BFT system","authors":"Missileny Xavier, W. Wasielesky Júnior, Bárbara Hostins, E. Bequé, Dariano Krummenauer","doi":"10.3856/vol50-issue2-fulltext-2777","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2777","url":null,"abstract":"Biofloc technology is a culture system that intensively uses microbial communities. Most bacteria can form aggregates around organic or inorganic surfaces. The high adsorption capacity, surface area, and flocculant properties of clay minerals make these compounds good candidates to stimulate biofloc formation, increase bacterial biomass, and improve nitrification. The objective of this study was to evaluate the addition of clay minerals to biofloc formation and its influence on the microbial community, nitrification, and zootechnical performance of Penaeus vannamei cultivated in a biofloc technology system. Experiment 1 involved the following treatments: control (without adding clay minerals), 10, 50, and 100 mg L-1, applied daily in the water. The treatments in experiment 2 were: control without product and inoculum, control without product and with inoculum, 50 mg L-1 clay mineral without inoculum and 50 mg L-1 clay mineral with inoculum. Both experiments with a density of 400 ind m-3. NO2 levels, total suspended solids, turbidity, differed significantly (P < 0.05) between treatments in experiment 1 and zootechnical performance parameters, where higher flocculant concentrations negatively affected these indices. In experiment 2, statistical differences were verified for NH3 and NO2 concentrations and the proximate composition of the bioflocs (P < 0.05), the addition of the inoculum of bioflocs influenced these results. In summary, the addition of clay minerals influenced biofloc formation, proximal composition, and the zootechnical performance of the cultivated shrimp but did not affect the abundance of microorganisms and nitrification.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46910135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.3856/vol50-issue2-fulltext-2776
M. Avendaño, Marcela Cantillánez
From 1999-2001, the Argopecten purpuratus population at La Rinconada was directly evaluated by monitoring larvae and their subsequent settlement on artificial collectors to determine their density, size structure, and abundance. An increase in specimens, doubling the number observed in 1997, occurred in 1999. However, reductions in the distribution area, specimens, and average size occurred in 2000 and 2001, reflecting anthropogenic impacts. Application of the growth parameters to the annual size structure indicated losses of 5.7 million individuals between 1999 and 2000 and 5 million between 2000 and 2001. The specimens increase over 90 mm, from 130,000 in 1997 to 2.6 million in 2001, explains the high larvae numbers found, with records of 17,667 and 21,756 in 1999; 16,100 in 2000; and 34,175 and 21,700 in 2001. A relationship between larval presence and postlarval settling could not be established; only the relationships between periods of larval abundance and those of maximum fixation could be observed. In conclusion, a viable solution to illegal fishing affecting the reserve with complementary or substitute measures for local fishers is urgently required.
{"title":"Argopecten purpuratus (Mollusca, Pectinidae) post-El Niño 1997-98 response in La Rinconada Marine Reserve (Antofagasta, Chile)","authors":"M. Avendaño, Marcela Cantillánez","doi":"10.3856/vol50-issue2-fulltext-2776","DOIUrl":"https://doi.org/10.3856/vol50-issue2-fulltext-2776","url":null,"abstract":"From 1999-2001, the Argopecten purpuratus population at La Rinconada was directly evaluated by monitoring larvae and their subsequent settlement on artificial collectors to determine their density, size structure, and abundance. An increase in specimens, doubling the number observed in 1997, occurred in 1999. However, reductions in the distribution area, specimens, and average size occurred in 2000 and 2001, reflecting anthropogenic impacts. Application of the growth parameters to the annual size structure indicated losses of 5.7 million individuals between 1999 and 2000 and 5 million between 2000 and 2001. The specimens increase over 90 mm, from 130,000 in 1997 to 2.6 million in 2001, explains the high larvae numbers found, with records of 17,667 and 21,756 in 1999; 16,100 in 2000; and 34,175 and 21,700 in 2001. A relationship between larval presence and postlarval settling could not be established; only the relationships between periods of larval abundance and those of maximum fixation could be observed. In conclusion, a viable solution to illegal fishing affecting the reserve with complementary or substitute measures for local fishers is urgently required.","PeriodicalId":49917,"journal":{"name":"Latin American Journal of Aquatic Research","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46554991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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