首页 > 最新文献

Journal of Membrane Biology最新文献

英文 中文
Membrane Proteins in Plant Salinity Stress Perception, Sensing, and Response. 植物盐胁迫感知、感知和响应中的膜蛋白。
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-04-01 DOI: 10.1007/s00232-023-00279-9
Sanhita Banik, Debajyoti Dutta

Plants have several mechanisms to endure salinity stress. The degree of salt tolerance varies significantly among different terrestrial crops. Proteins at the plant's cell wall and membrane mediate different physiological roles owing to their critical positioning between two distinct environments. A specific membrane protein is responsible for a single type of activity, such as a specific group of ion transport or a similar group of small molecule binding to exert multiple cellular effects. During salinity stress in plants, membrane protein functions: ion homeostasis, signal transduction, redox homeostasis, and solute transport are essential for stress perception, signaling, and recovery. Therefore, comprehensive knowledge about plant membrane proteins is essential to modulate crop salinity tolerance. This review gives a detailed overview of the membrane proteins involved in plant salinity stress highlighting the recent findings. Also, it discusses the role of solute transporters, accessory polypeptides, and proteins in salinity tolerance. Finally, some aspects of membrane proteins are discussed with potential applications to developing salt tolerance in crops.

植物有几种机制来忍受盐胁迫。不同陆地作物的耐盐程度差异较大。植物细胞壁和细胞膜上的蛋白质由于其在两种不同环境之间的关键位置而介导不同的生理作用。一种特定的膜蛋白负责一种单一类型的活动,例如一组特定的离子运输或一组类似的小分子结合来发挥多种细胞作用。在盐度胁迫下,植物膜蛋白的功能:离子稳态、信号转导、氧化还原稳态和溶质转运对胁迫感知、信号传导和恢复至关重要。因此,全面了解植物膜蛋白对调节作物耐盐性至关重要。本文对植物盐胁迫中膜蛋白的研究进展进行了综述。此外,还讨论了溶质转运体、辅助多肽和蛋白质在耐盐性中的作用。最后,讨论了膜蛋白在作物耐盐性方面的应用前景。
{"title":"Membrane Proteins in Plant Salinity Stress Perception, Sensing, and Response.","authors":"Sanhita Banik,&nbsp;Debajyoti Dutta","doi":"10.1007/s00232-023-00279-9","DOIUrl":"https://doi.org/10.1007/s00232-023-00279-9","url":null,"abstract":"<p><p>Plants have several mechanisms to endure salinity stress. The degree of salt tolerance varies significantly among different terrestrial crops. Proteins at the plant's cell wall and membrane mediate different physiological roles owing to their critical positioning between two distinct environments. A specific membrane protein is responsible for a single type of activity, such as a specific group of ion transport or a similar group of small molecule binding to exert multiple cellular effects. During salinity stress in plants, membrane protein functions: ion homeostasis, signal transduction, redox homeostasis, and solute transport are essential for stress perception, signaling, and recovery. Therefore, comprehensive knowledge about plant membrane proteins is essential to modulate crop salinity tolerance. This review gives a detailed overview of the membrane proteins involved in plant salinity stress highlighting the recent findings. Also, it discusses the role of solute transporters, accessory polypeptides, and proteins in salinity tolerance. Finally, some aspects of membrane proteins are discussed with potential applications to developing salt tolerance in crops.</p>","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"256 2","pages":"109-124"},"PeriodicalIF":2.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9682050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Functional Potassium Channels in Macrophages. 巨噬细胞中的功能性钾通道。
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-04-01 DOI: 10.1007/s00232-022-00276-4
Qiaoyan Man, Zhe Gao, Kuihao Chen

Macrophages are the predominant component of innate immunity, which is an important protective barrier of our body. Macrophages are present in all organs and tissues of the body, their main functions include immune surveillance, bacterial killing, tissue remodeling and repair, and clearance of cell debris. In addition, macrophages can present antigens to T cells and facilitate inflammatory response by releasing cytokines. Macrophages are of high concern due to their crucial roles in multiple physiological processes. In recent years, new advances are emerging after great efforts have been made to explore the mechanisms of macrophage activation. Ion channel is a class of multimeric transmembrane protein that allows specific ions to go through cell membrane. The flow of ions through ion channel between inside and outside of cell membrane is required for maintaining cell morphology and intracellular signal transduction. Expressions of various ion channels in macrophages have been detected. The roles of ion channels in macrophage activation are gradually caught attention. K+ channels are the most studied channels in immune system. However, very few of published papers reviewed the studies of K+ channels on macrophages. Here, we will review the four types of K+ channels that are expressed in macrophages: voltage-gated K+ channel, calcium-activated K+ channel, inwardly rectifying K+ channel and two-pore domain K+ channel.

巨噬细胞是先天免疫的主要组成部分,是机体重要的保护屏障。巨噬细胞存在于人体的所有器官和组织中,其主要功能包括免疫监视、细菌杀伤、组织重塑和修复以及清除细胞碎片。此外,巨噬细胞可以向T细胞呈递抗原,并通过释放细胞因子促进炎症反应。巨噬细胞因其在多种生理过程中的重要作用而备受关注。近年来,对巨噬细胞活化机制的研究不断取得新进展。离子通道是一类多聚体跨膜蛋白,允许特定离子通过细胞膜。细胞膜内外离子通道中离子的流动是维持细胞形态和细胞内信号转导所必需的。检测了巨噬细胞中各种离子通道的表达。离子通道在巨噬细胞活化中的作用逐渐受到关注。K+通道是免疫系统中研究最多的通道。然而,对于巨噬细胞中K+通道的研究,目前发表的文献很少。在这里,我们将回顾巨噬细胞中表达的四种类型的K+通道:电压门控K+通道、钙激活K+通道、内纠偏K+通道和双孔域K+通道。
{"title":"Functional Potassium Channels in Macrophages.","authors":"Qiaoyan Man,&nbsp;Zhe Gao,&nbsp;Kuihao Chen","doi":"10.1007/s00232-022-00276-4","DOIUrl":"https://doi.org/10.1007/s00232-022-00276-4","url":null,"abstract":"<p><p>Macrophages are the predominant component of innate immunity, which is an important protective barrier of our body. Macrophages are present in all organs and tissues of the body, their main functions include immune surveillance, bacterial killing, tissue remodeling and repair, and clearance of cell debris. In addition, macrophages can present antigens to T cells and facilitate inflammatory response by releasing cytokines. Macrophages are of high concern due to their crucial roles in multiple physiological processes. In recent years, new advances are emerging after great efforts have been made to explore the mechanisms of macrophage activation. Ion channel is a class of multimeric transmembrane protein that allows specific ions to go through cell membrane. The flow of ions through ion channel between inside and outside of cell membrane is required for maintaining cell morphology and intracellular signal transduction. Expressions of various ion channels in macrophages have been detected. The roles of ion channels in macrophage activation are gradually caught attention. K<sup>+</sup> channels are the most studied channels in immune system. However, very few of published papers reviewed the studies of K<sup>+</sup> channels on macrophages. Here, we will review the four types of K<sup>+</sup> channels that are expressed in macrophages: voltage-gated K<sup>+</sup> channel, calcium-activated K<sup>+</sup> channel, inwardly rectifying K<sup>+</sup> channel and two-pore domain K<sup>+</sup> channel.</p>","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"256 2","pages":"175-187"},"PeriodicalIF":2.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9685028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Chloride Channel-3 (ClC-3) Modifies the Trafficking of Leucine-Rich Repeat-Containing 8A (LRRC8A) Anion Channels. 氯离子通道-3(ClC-3)改变了含亮氨酸富重复序列 8A (LRRC8A) 阴离子通道的通路
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-04-01 Epub Date: 2022-11-02 DOI: 10.1007/s00232-022-00271-9
Ryan J Stark, Hong N Nguyen, Matthew K Bacon, Jeffrey C Rohrbough, Hyehun Choi, Fred S Lamb

Chloride channel-3 (ClC-3) Cl-/H+ antiporters and leucine-rich repeat-containing 8 (LRRC8) family anion channels have both been associated with volume-regulated anion currents (VRACs). VRACs are often altered in ClC-3 null cells but are absent in LRRC8A null cells. To explore the relationship between ClC-3, LRRC8A, and VRAC we localized tagged proteins in human epithelial kidney (HEK293) cells using multimodal microscopy. Expression of ClC-3-GFP induced large multivesicular bodies (MVBs) with ClC-3 in the delimiting membrane. LRRC8A-RFP localized to the plasma membrane and to small cytoplasmic vesicles. Co-expression demonstrated co-localization in small, highly mobile cytoplasmic vesicles that associated with the early endosomal marker Rab5A. However, most of the small LRRC8A-positive vesicles were constrained within large MVBs with abundant ClC-3 in the delimiting membrane. Dominant negative (S34A) Rab5A prevented ClC-3 overexpression from creating enlarged MVBs, while constitutively active (Q79L) Rab5A enhanced this phenotype. Thus, ClC-3 and LRRC8A are endocytosed together but independently sorted in Rab5A MVBs. Subsequently, LRRC8A-labeled vesicles were sorted to MVBs labeled by Rab27A and B exosomal compartment markers, but not to Rab11 recycling endosomes. VRAC currents were significantly larger in ClC-3 null HEK293 cells. This work demonstrates dependence of LRRC8A trafficking on ClC-3 which may explain the association between ClC-3 and VRACs.

氯离子通道-3(ClC-3)Cl-/H+ 反转运体和富含亮氨酸重复的 8(LRRC8)家族阴离子通道都与体积调节阴离子电流(VRACs)有关。在 ClC-3 缺失的细胞中,VRACs 通常会发生改变,但在 LRRC8A 缺失的细胞中却不存在。为了探索 ClC-3、LRRC8A 和 VRAC 之间的关系,我们使用多模态显微镜定位了人上皮肾脏(HEK293)细胞中的标记蛋白。ClC-3-GFP 的表达诱导了大的多囊体(MVB),ClC-3 位于分界膜上。LRRC8A-RFP 定位于质膜和细胞质小泡。共表达表明,LRRC8A-RFP 与与早期内体标记物 Rab5A 相关的高流动性小细胞质囊泡共定位。然而,大多数 LRRC8A 阳性的小囊泡都被限制在大的 MVB 中,其分界膜上有丰富的 ClC-3。显性阴性(S34A)Rab5A 阻止了 ClC-3 的过量表达,使其无法形成增大的 MVB,而组成型活性(Q79L)Rab5A 则增强了这种表型。因此,ClC-3 和 LRRC8A 一起被内吞,但在 Rab5A MVB 中独立分选。随后,LRRC8A 标记的囊泡被分拣到由 Rab27A 和 B 外泌体区室标记的 MVB 中,但没有被分拣到 Rab11 循环内体中。在 ClC-3 缺失的 HEK293 细胞中,VRAC 电流明显增大。这项工作证明了 LRRC8A 的贩运依赖于 ClC-3,这可能解释了 ClC-3 与 VRAC 之间的关联。
{"title":"Chloride Channel-3 (ClC-3) Modifies the Trafficking of Leucine-Rich Repeat-Containing 8A (LRRC8A) Anion Channels.","authors":"Ryan J Stark, Hong N Nguyen, Matthew K Bacon, Jeffrey C Rohrbough, Hyehun Choi, Fred S Lamb","doi":"10.1007/s00232-022-00271-9","DOIUrl":"10.1007/s00232-022-00271-9","url":null,"abstract":"<p><p>Chloride channel-3 (ClC-3) Cl<sup>-</sup>/H<sup>+</sup> antiporters and leucine-rich repeat-containing 8 (LRRC8) family anion channels have both been associated with volume-regulated anion currents (VRACs). VRACs are often altered in ClC-3 null cells but are absent in LRRC8A null cells. To explore the relationship between ClC-3, LRRC8A, and VRAC we localized tagged proteins in human epithelial kidney (HEK293) cells using multimodal microscopy. Expression of ClC-3-GFP induced large multivesicular bodies (MVBs) with ClC-3 in the delimiting membrane. LRRC8A-RFP localized to the plasma membrane and to small cytoplasmic vesicles. Co-expression demonstrated co-localization in small, highly mobile cytoplasmic vesicles that associated with the early endosomal marker Rab5A. However, most of the small LRRC8A-positive vesicles were constrained within large MVBs with abundant ClC-3 in the delimiting membrane. Dominant negative (S34A) Rab5A prevented ClC-3 overexpression from creating enlarged MVBs, while constitutively active (Q79L) Rab5A enhanced this phenotype. Thus, ClC-3 and LRRC8A are endocytosed together but independently sorted in Rab5A MVBs. Subsequently, LRRC8A-labeled vesicles were sorted to MVBs labeled by Rab27A and B exosomal compartment markers, but not to Rab11 recycling endosomes. VRAC currents were significantly larger in ClC-3 null HEK293 cells. This work demonstrates dependence of LRRC8A trafficking on ClC-3 which may explain the association between ClC-3 and VRACs.</p>","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"256 2","pages":"125-135"},"PeriodicalIF":2.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085862/pdf/nihms-1879539.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10055104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of Cholesterol and its Biosynthetic Precursors on Membrane Organization and Dynamics: A Fluorescence Approach. 胆固醇及其生物合成前体在膜组织和动力学中的作用:荧光方法。
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-04-01 DOI: 10.1007/s00232-023-00278-w
Sandeep Shrivastava, Yamuna Devi Paila, Amitabha Chattopadhyay

Cholesterol is the most representative sterol present in membranes of higher eukaryotes, and is the end product of a long and multistep biosynthetic pathway. Lathosterol and zymosterol are biosynthetic precursors of cholesterol in Kandutsch-Russell and Bloch pathways, respectively. Lathosterol differs with cholesterol merely in the position of the double bond in the sterol ring, whereas zymosterol differs with cholesterol in position and number of double bonds. In this work, we have monitored the effect of cholesterol and its biosynthetic precursors (lathosterol and zymosterol) on membrane organization and dynamics in fluid and gel phase membranes. Toward this goal, we have utilized two fluorescent membrane probes, DPH and its cationic derivative TMA-DPH. Our results using these probes show that cholesterol and its biosynthetic precursors (lathosterol and zymosterol) exhibit similar trend in maintaining membrane organization and dynamics (as reported by fluorescence anisotropy and apparent rotational correlation time), in fluid phase POPC membranes. Notably, although lathosterol and zymosterol show similar trend in maintaining membrane organization and dynamics, the corresponding change for cholesterol is different in gel phase DPPC membranes. These results demonstrate that the position and number of double bonds in sterols is an important determinant in maintaining membrane physical properties. Our results assume significance since accumulation of precursors of cholesterol have been reported to be associated with severe pathological conditions.

胆固醇是高等真核生物膜中最具代表性的甾醇,是一个漫长而多步骤的生物合成途径的最终产物。肝甾醇和酶甾醇分别是Kandutsch-Russell和Bloch途径中胆固醇的生物合成前体。肝甾醇与胆固醇的区别仅仅在于固醇环中双键的位置,而酶甾醇与胆固醇的区别在于双键的位置和数量。在这项工作中,我们监测了胆固醇及其生物合成前体(胆甾醇和酶甾醇)对流体和凝胶相膜的膜组织和动力学的影响。为了实现这一目标,我们利用了两种荧光膜探针,DPH及其阳离子衍生物TMA-DPH。我们使用这些探针的结果表明,在液相POPC膜中,胆固醇及其生物合成前体(胆甾醇和酶甾醇)在维持膜组织和动力学(荧光各向异性和表观旋转相关时间)方面表现出相似的趋势。值得注意的是,尽管胆甾醇和酶甾醇在维持膜组织和动力学方面表现出相似的趋势,但胆固醇在凝胶相DPPC膜中相应的变化是不同的。这些结果表明,甾醇中双键的位置和数量是维持膜物理性质的重要决定因素。我们的研究结果具有重要意义,因为据报道,胆固醇前体的积累与严重的病理状况有关。
{"title":"Role of Cholesterol and its Biosynthetic Precursors on Membrane Organization and Dynamics: A Fluorescence Approach.","authors":"Sandeep Shrivastava,&nbsp;Yamuna Devi Paila,&nbsp;Amitabha Chattopadhyay","doi":"10.1007/s00232-023-00278-w","DOIUrl":"https://doi.org/10.1007/s00232-023-00278-w","url":null,"abstract":"<p><p>Cholesterol is the most representative sterol present in membranes of higher eukaryotes, and is the end product of a long and multistep biosynthetic pathway. Lathosterol and zymosterol are biosynthetic precursors of cholesterol in Kandutsch-Russell and Bloch pathways, respectively. Lathosterol differs with cholesterol merely in the position of the double bond in the sterol ring, whereas zymosterol differs with cholesterol in position and number of double bonds. In this work, we have monitored the effect of cholesterol and its biosynthetic precursors (lathosterol and zymosterol) on membrane organization and dynamics in fluid and gel phase membranes. Toward this goal, we have utilized two fluorescent membrane probes, DPH and its cationic derivative TMA-DPH. Our results using these probes show that cholesterol and its biosynthetic precursors (lathosterol and zymosterol) exhibit similar trend in maintaining membrane organization and dynamics (as reported by fluorescence anisotropy and apparent rotational correlation time), in fluid phase POPC membranes. Notably, although lathosterol and zymosterol show similar trend in maintaining membrane organization and dynamics, the corresponding change for cholesterol is different in gel phase DPPC membranes. These results demonstrate that the position and number of double bonds in sterols is an important determinant in maintaining membrane physical properties. Our results assume significance since accumulation of precursors of cholesterol have been reported to be associated with severe pathological conditions.</p>","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"256 2","pages":"189-197"},"PeriodicalIF":2.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9672870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface Modification of Poly(tetrafluoroethylene) (PTFE) Membranes 聚四氟乙烯(PTFE)膜的表面改性
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-02-28 DOI: 10.14579/membrane_journal.2023.33.1.1
Jun Kyu Jang, Chaewon Youn, H. Park
{"title":"Surface Modification of Poly(tetrafluoroethylene) (PTFE) Membranes","authors":"Jun Kyu Jang, Chaewon Youn, H. Park","doi":"10.14579/membrane_journal.2023.33.1.1","DOIUrl":"https://doi.org/10.14579/membrane_journal.2023.33.1.1","url":null,"abstract":"","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"70 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86257000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modeling of a Dynamic Membrane Filtration Process Using ANN and SVM to Predict the Permeate Flux 基于神经网络和支持向量机的动态膜过滤过程建模及渗透通量预测
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-02-28 DOI: 10.14579/membrane_journal.2023.33.1.34
Soufyane Ladeg, M. Moussaoui, M. Laidi, N. Moulai-Mostefa
{"title":"Modeling of a Dynamic Membrane Filtration Process Using ANN and SVM to Predict the Permeate Flux","authors":"Soufyane Ladeg, M. Moussaoui, M. Laidi, N. Moulai-Mostefa","doi":"10.14579/membrane_journal.2023.33.1.34","DOIUrl":"https://doi.org/10.14579/membrane_journal.2023.33.1.34","url":null,"abstract":"","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"22 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76598465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CO2 Separation Performance of PEBAX Mixed Matrix Membrane Using PEI-GO@ZIF-8 as Filler PEI-GO@ZIF-8填料PEBAX混合基质膜的CO2分离性能
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-02-28 DOI: 10.14579/membrane_journal.2023.33.1.23
Eun. Yi, R. Hong, Hyun Kyung Lee
{"title":"CO2 Separation Performance of PEBAX Mixed Matrix Membrane Using PEI-GO@ZIF-8 as Filler","authors":"Eun. Yi, R. Hong, Hyun Kyung Lee","doi":"10.14579/membrane_journal.2023.33.1.23","DOIUrl":"https://doi.org/10.14579/membrane_journal.2023.33.1.23","url":null,"abstract":"","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"22 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80703395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Enhanced Electrochemical Performance of Lithium Metal Batteries through the Piezoelectric Protective Layer 利用压电保护层增强锂金属电池的电化学性能
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-02-28 DOI: 10.14579/membrane_journal.2023.33.1.13
Dae-Ung Park, W. Shin, H. Sohn
{"title":"The Enhanced Electrochemical Performance of Lithium Metal Batteries through the Piezoelectric Protective Layer","authors":"Dae-Ung Park, W. Shin, H. Sohn","doi":"10.14579/membrane_journal.2023.33.1.13","DOIUrl":"https://doi.org/10.14579/membrane_journal.2023.33.1.13","url":null,"abstract":"","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"65 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82574565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perspective: Analysis of Conditions for High-efficiency/Eco-friendly Energy Production Devices for Smart Cities 视角:智慧城市高效/环保能源生产设备条件分析
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-02-28 DOI: 10.14579/membrane_journal.2023.33.1.46
S. Kang, Jeong Uk Kim
{"title":"Perspective: Analysis of Conditions for High-efficiency/Eco-friendly Energy Production Devices for Smart Cities","authors":"S. Kang, Jeong Uk Kim","doi":"10.14579/membrane_journal.2023.33.1.46","DOIUrl":"https://doi.org/10.14579/membrane_journal.2023.33.1.46","url":null,"abstract":"","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"247 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74708048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Caveolae Microdomains Mediate STAT5 Signaling Induced by Insulin in MCF-7 Breast Cancer Cells. MCF-7乳腺癌细胞中小泡微结构域介导胰岛素诱导的STAT5信号传导
IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-02-01 DOI: 10.1007/s00232-022-00253-x
Rocio Castillo-Sanchez, Pedro Cortes-Reynosa, Mario Lopez-Perez, Alejandra Garcia-Hernandez, Eduardo Perez Salazar

Caveolae are small plasma membrane invaginations constituted for membrane proteins namely caveolins and cytosolic proteins termed cavins, which can occupy up to 50% of the surface of mammalian cells. The caveolae have been involved with a variety of cellular processes including regulation of cellular signaling. Insulin is a hormone that mediates a variety of physiological processes through activation of insulin receptor (IR), which is a tyrosine kinase receptor expressed in all mammalian tissues. Insulin induces activation of signal transducers and activators of transcription (STAT) family members including STAT5. In this study, we demonstrate, for the first time, that insulin induces phosphorylation of STAT5 at tyrosine-694 (STAT5-Tyr(P)694), STAT5 nuclear accumulation and an increase in STAT5-DNA complex formation in MCF-7 breast cancer cells. Insulin also induces nuclear accumulation of STAT5-Tyr(P)694, caveolin-1, and IR in MCF-7 cells. STAT5 nuclear accumulation and the increase of STAT5-DNA complex formation require the integrity of caveolae and microtubule network. Moreover, insulin induces an increase and nuclear accumulation of STAT5-Tyr(P)694 in MDA-MB-231 breast cancer cells. In conclusion, results demonstrate that caveolae and microtubule network play an important role in STAT5-Tyr(P)694, STAT5 nuclear accumulation and STAT5-DNA complex formation induced by insulin in breast cancer cells.

小泡是由膜蛋白即小泡蛋白和称为小泡蛋白的胞质蛋白构成的小的质膜内陷,它们可以占据哺乳动物细胞表面的50%。小泡参与了多种细胞过程,包括细胞信号的调节。胰岛素是一种通过激活胰岛素受体(Insulin receptor, IR)介导多种生理过程的激素,IR是一种酪氨酸激酶受体,在哺乳动物所有组织中均有表达。胰岛素诱导包括STAT5在内的信号转导和转录激活因子(STAT)家族成员的激活。在这项研究中,我们首次证明了胰岛素诱导MCF-7乳腺癌细胞中酪氨酸-694位点的STAT5磷酸化(STAT5- tyr (P)694)、STAT5核积累和STAT5- dna复合物形成增加。胰岛素也诱导MCF-7细胞中STAT5-Tyr(P)694、caveolin-1和IR的核积累。STAT5核的积累和STAT5- dna复合物形成的增加需要小泡和微管网络的完整性。此外,胰岛素诱导MDA-MB-231乳腺癌细胞中STAT5-Tyr(P)694的增加和核积累。综上所述,胰岛素诱导乳腺癌细胞中STAT5- tyr (P)694、STAT5核积累和STAT5- dna复合物形成的过程中,小泡和微管网络发挥了重要作用。
{"title":"Caveolae Microdomains Mediate STAT5 Signaling Induced by Insulin in MCF-7 Breast Cancer Cells.","authors":"Rocio Castillo-Sanchez,&nbsp;Pedro Cortes-Reynosa,&nbsp;Mario Lopez-Perez,&nbsp;Alejandra Garcia-Hernandez,&nbsp;Eduardo Perez Salazar","doi":"10.1007/s00232-022-00253-x","DOIUrl":"https://doi.org/10.1007/s00232-022-00253-x","url":null,"abstract":"<p><p>Caveolae are small plasma membrane invaginations constituted for membrane proteins namely caveolins and cytosolic proteins termed cavins, which can occupy up to 50% of the surface of mammalian cells. The caveolae have been involved with a variety of cellular processes including regulation of cellular signaling. Insulin is a hormone that mediates a variety of physiological processes through activation of insulin receptor (IR), which is a tyrosine kinase receptor expressed in all mammalian tissues. Insulin induces activation of signal transducers and activators of transcription (STAT) family members including STAT5. In this study, we demonstrate, for the first time, that insulin induces phosphorylation of STAT5 at tyrosine-694 (STAT5-Tyr(P)<sup>694</sup>), STAT5 nuclear accumulation and an increase in STAT5-DNA complex formation in MCF-7 breast cancer cells. Insulin also induces nuclear accumulation of STAT5-Tyr(P)<sup>694</sup>, caveolin-1, and IR in MCF-7 cells. STAT5 nuclear accumulation and the increase of STAT5-DNA complex formation require the integrity of caveolae and microtubule network. Moreover, insulin induces an increase and nuclear accumulation of STAT5-Tyr(P)<sup>694</sup> in MDA-MB-231 breast cancer cells. In conclusion, results demonstrate that caveolae and microtubule network play an important role in STAT5-Tyr(P)<sup>694</sup>, STAT5 nuclear accumulation and STAT5-DNA complex formation induced by insulin in breast cancer cells.</p>","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"256 1","pages":"79-90"},"PeriodicalIF":2.4,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10641472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Membrane Biology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1