Abstract. Numerous studies on prolific sheep breeds have shown that the transforming growth factor beta (TGF-β) superfamily members, including bone morphogenetic protein receptor type 1B (BMPR1B), bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9), are the essential regulators of ovulation rate and litter size. In total, 11 known mutations (1 in BMPR1B, 6 in BMP15 and 4 in GDF9) are able to increase the ovulation rate and litter size, respectively. In this study, the genomic DNA was isolated from 512 high-prolificacy sheep (including the Small-tailed Han, Hu and Wadi sheep breeds) and 164 low-prolificacy sheep (Tan sheep), and genotyping of the specific mutations of the three fecundity-related genes was carried out by sequencing. The results showed that the FecB mutation in BMPR1B was detected in all four sheep breeds, and the frequency of B allele was significantly higher in the high-prolificacy breeds than that in the low-prolificacy breed (P<0.001). A novel mutation, c.T755C (named S1), was found in BMP15 from the four sheep breeds. However, known mutations such as FecXI, FecXH, FecXB, FecXG, FecXL and FecXR were not detected in these breeds. Three known loci (G1, G3 and G4) and a new mutation, c.A1515G (named S2), were found in GDF9, and the other three known mutations (FecGH (G8), FecGE and FecTT) were not detected in all four sheep breeds. The genotype distribution at the G1 and G4 loci had significant differences between the low-prolificacy sheep breed and the other three high-prolificacy sheep breeds. There was no difference in the genotype distribution at the G1 and G4 loci between the three high-prolificacy sheep breeds. Haplotype analysis of the four polymorphic loci in GDF9 suggested that H4 (GGAA) was the preponderant haplotype in the three high-prolificacy sheep breeds, but H1 (GGGG) was in the low-prolificacy sheep breed. These results preliminarily showed that the BMPR1B and GDF9 might be major genes influencing the prolificacy of Chinese sheep breeds.
{"title":"Polymorphisms of the BMPR1B, BMP15 and GDF9 fecundity genes in four Chinese sheep breeds","authors":"Jinxin Wang, Yufang Liu, Siwu Guo, R. Di, Xiangyu Wang, Xiaoyun He, Mingxing Chu","doi":"10.5194/aab-67-51-2024","DOIUrl":"https://doi.org/10.5194/aab-67-51-2024","url":null,"abstract":"Abstract. Numerous studies on prolific sheep breeds have shown that the transforming growth factor beta (TGF-β) superfamily members, including bone morphogenetic protein receptor type 1B (BMPR1B), bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9), are the essential regulators of ovulation rate and litter size. In total, 11 known mutations (1 in BMPR1B, 6 in BMP15 and 4 in GDF9) are able to increase the ovulation rate and litter size, respectively. In this study, the genomic DNA was isolated from 512 high-prolificacy sheep (including the Small-tailed Han, Hu and Wadi sheep breeds) and 164 low-prolificacy sheep (Tan sheep), and genotyping of the specific mutations of the three fecundity-related genes was carried out by sequencing. The results showed that the FecB mutation in BMPR1B was detected in all four sheep breeds, and the frequency of B allele was significantly higher in the high-prolificacy breeds than that in the low-prolificacy breed (P<0.001). A novel mutation, c.T755C (named S1), was found in BMP15 from the four sheep breeds. However, known mutations such as FecXI, FecXH, FecXB, FecXG, FecXL and FecXR were not detected in these breeds. Three known loci (G1, G3 and G4) and a new mutation, c.A1515G (named S2), were found in GDF9, and the other three known mutations (FecGH (G8), FecGE and FecTT) were not detected in all four sheep breeds. The genotype distribution at the G1 and G4 loci had significant differences between the low-prolificacy sheep breed and the other three high-prolificacy sheep breeds. There was no difference in the genotype distribution at the G1 and G4 loci between the three high-prolificacy sheep breeds. Haplotype analysis of the four polymorphic loci in GDF9 suggested that H4 (GGAA) was the preponderant haplotype in the three high-prolificacy sheep breeds, but H1 (GGGG) was in the low-prolificacy sheep breed. These results preliminarily showed that the BMPR1B and GDF9 might be major genes influencing the prolificacy of Chinese sheep breeds.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"134 3-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139855389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Antunović, B. Mioč, Željka Klir Šalavardić, I. Širić, V. Držaić, Nataša Mikulec, Adela Krivohlavek, J. Novoselec
Abstract. In recent years, there has been globally increasing interest in dairy sheep breeding, including Lacaune sheep, which is supported by a high demand for sheep's milk on the market. This paper elaborates on the influence of a sheep's lactation stage on the physical and chemical properties, hygienic quality and content of fatty acids in milk produced by Lacaune sheep kept in intensive breeding. The research was conducted on 30 Lacaune sheep, which were tested in the early (60th day), middle (120th day) and late (180th day) stages of lactation. Density, freezing point and titration acidity were determined by applying the infrared spectrometry method, and indicators of the hygienic quality of milk, such as somatic cell count (SCC), were determined by the fluoro-opto-electronic method, and the total count of aerobic mesophilic bacteria (CFU) was determined by the flow cytometry method. The fatty acid profile of feed and milk was obtained by gas–liquid chromatography. Depending on the stage of lactation, results referring to the chemical composition of Lacaune sheep's milk showed a significant increase in the content of fat, protein, total dry matter and casein together with a significant decrease in the content of lactose in the late stage of lactation. There was also a significant increase confirmed for the concentration of urea and the freezing point in milk along with the lactation progress. Depending on the stage of lactation, milk yield in Lacaune sheep significantly decreased as lactation progressed. Analysis of the fatty acid composition in milk of Lacaune sheep proved a significant decrease in the concentrations of C4 : 0, C6 : 0, C11 : 0, C12 : 0, C13 : 0, C15 : 0, C17 : 1, C18 : 2n6 and C18 : 3n6 as well as the n6 concentrations and the n6 / n3 ratio. The opposite trend was observed for concentrations of C10 : 0, C14 : 1, C16 : 0, C16 : 1, C18 : 0, C20 : 2, C18 : 3n3, C20 : 3n6, C20 : 5n3 and C22 : 6n3 as well as for the n3 concentrations. When compared to the early lactation stage, the C18 : 3n6 and n6 concentrations were significantly lower in the late lactation stage, while the C20 : 2 and C20 : 5n3 concentrations were significantly lower in the middle lactation stage when compared to the late lactation stage. There were many significant positive and negative correlations determined between the researched properties of milk. The research results obtained with Lacaune sheep's milk can be compared to the results of other studies, except for the lower content of milk fat. This confirms the good adaptability of Lacaune sheep to different breeding conditions and the necessity to provide sheep with quality pastures for grazing.
{"title":"Physical and chemical properties, hygienic quality and fatty acid profile in milk of lactating Lacaune dairy sheep","authors":"Z. Antunović, B. Mioč, Željka Klir Šalavardić, I. Širić, V. Držaić, Nataša Mikulec, Adela Krivohlavek, J. Novoselec","doi":"10.5194/aab-67-37-2024","DOIUrl":"https://doi.org/10.5194/aab-67-37-2024","url":null,"abstract":"Abstract. In recent years, there has been globally increasing interest in dairy sheep breeding, including Lacaune sheep, which is supported by a high demand for sheep's milk on the market. This paper elaborates on the influence of a sheep's lactation stage on the physical and chemical properties, hygienic quality and content of fatty acids in milk produced by Lacaune sheep kept in intensive breeding. The research was conducted on 30 Lacaune sheep, which were tested in the early (60th day), middle (120th day) and late (180th day) stages of lactation. Density, freezing point and titration acidity were determined by applying the infrared spectrometry method, and indicators of the hygienic quality of milk, such as somatic cell count (SCC), were determined by the fluoro-opto-electronic method, and the total count of aerobic mesophilic bacteria (CFU) was determined by the flow cytometry method. The fatty acid profile of feed and milk was obtained by gas–liquid chromatography. Depending on the stage of lactation, results referring to the chemical composition of Lacaune sheep's milk showed a significant increase in the content of fat, protein, total dry matter and casein together with a significant decrease in the content of lactose in the late stage of lactation. There was also a significant increase confirmed for the concentration of urea and the freezing point in milk along with the lactation progress. Depending on the stage of lactation, milk yield in Lacaune sheep significantly decreased as lactation progressed. Analysis of the fatty acid composition in milk of Lacaune sheep proved a significant decrease in the concentrations of C4 : 0, C6 : 0, C11 : 0, C12 : 0, C13 : 0, C15 : 0, C17 : 1, C18 : 2n6 and C18 : 3n6 as well as the n6 concentrations and the n6 / n3 ratio. The opposite trend was observed for concentrations of C10 : 0, C14 : 1, C16 : 0, C16 : 1, C18 : 0, C20 : 2, C18 : 3n3, C20 : 3n6, C20 : 5n3 and C22 : 6n3 as well as for the n3 concentrations. When compared to the early lactation stage, the C18 : 3n6 and n6 concentrations were significantly lower in the late lactation stage, while the C20 : 2 and C20 : 5n3 concentrations were significantly lower in the middle lactation stage when compared to the late lactation stage. There were many significant positive and negative correlations determined between the researched properties of milk. The research results obtained with Lacaune sheep's milk can be compared to the results of other studies, except for the lower content of milk fat. This confirms the good adaptability of Lacaune sheep to different breeding conditions and the necessity to provide sheep with quality pastures for grazing.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"40 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139869680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Antunović, B. Mioč, Željka Klir Šalavardić, I. Širić, V. Držaić, Nataša Mikulec, Adela Krivohlavek, J. Novoselec
Abstract. In recent years, there has been globally increasing interest in dairy sheep breeding, including Lacaune sheep, which is supported by a high demand for sheep's milk on the market. This paper elaborates on the influence of a sheep's lactation stage on the physical and chemical properties, hygienic quality and content of fatty acids in milk produced by Lacaune sheep kept in intensive breeding. The research was conducted on 30 Lacaune sheep, which were tested in the early (60th day), middle (120th day) and late (180th day) stages of lactation. Density, freezing point and titration acidity were determined by applying the infrared spectrometry method, and indicators of the hygienic quality of milk, such as somatic cell count (SCC), were determined by the fluoro-opto-electronic method, and the total count of aerobic mesophilic bacteria (CFU) was determined by the flow cytometry method. The fatty acid profile of feed and milk was obtained by gas–liquid chromatography. Depending on the stage of lactation, results referring to the chemical composition of Lacaune sheep's milk showed a significant increase in the content of fat, protein, total dry matter and casein together with a significant decrease in the content of lactose in the late stage of lactation. There was also a significant increase confirmed for the concentration of urea and the freezing point in milk along with the lactation progress. Depending on the stage of lactation, milk yield in Lacaune sheep significantly decreased as lactation progressed. Analysis of the fatty acid composition in milk of Lacaune sheep proved a significant decrease in the concentrations of C4 : 0, C6 : 0, C11 : 0, C12 : 0, C13 : 0, C15 : 0, C17 : 1, C18 : 2n6 and C18 : 3n6 as well as the n6 concentrations and the n6 / n3 ratio. The opposite trend was observed for concentrations of C10 : 0, C14 : 1, C16 : 0, C16 : 1, C18 : 0, C20 : 2, C18 : 3n3, C20 : 3n6, C20 : 5n3 and C22 : 6n3 as well as for the n3 concentrations. When compared to the early lactation stage, the C18 : 3n6 and n6 concentrations were significantly lower in the late lactation stage, while the C20 : 2 and C20 : 5n3 concentrations were significantly lower in the middle lactation stage when compared to the late lactation stage. There were many significant positive and negative correlations determined between the researched properties of milk. The research results obtained with Lacaune sheep's milk can be compared to the results of other studies, except for the lower content of milk fat. This confirms the good adaptability of Lacaune sheep to different breeding conditions and the necessity to provide sheep with quality pastures for grazing.
{"title":"Physical and chemical properties, hygienic quality and fatty acid profile in milk of lactating Lacaune dairy sheep","authors":"Z. Antunović, B. Mioč, Željka Klir Šalavardić, I. Širić, V. Držaić, Nataša Mikulec, Adela Krivohlavek, J. Novoselec","doi":"10.5194/aab-67-37-2024","DOIUrl":"https://doi.org/10.5194/aab-67-37-2024","url":null,"abstract":"Abstract. In recent years, there has been globally increasing interest in dairy sheep breeding, including Lacaune sheep, which is supported by a high demand for sheep's milk on the market. This paper elaborates on the influence of a sheep's lactation stage on the physical and chemical properties, hygienic quality and content of fatty acids in milk produced by Lacaune sheep kept in intensive breeding. The research was conducted on 30 Lacaune sheep, which were tested in the early (60th day), middle (120th day) and late (180th day) stages of lactation. Density, freezing point and titration acidity were determined by applying the infrared spectrometry method, and indicators of the hygienic quality of milk, such as somatic cell count (SCC), were determined by the fluoro-opto-electronic method, and the total count of aerobic mesophilic bacteria (CFU) was determined by the flow cytometry method. The fatty acid profile of feed and milk was obtained by gas–liquid chromatography. Depending on the stage of lactation, results referring to the chemical composition of Lacaune sheep's milk showed a significant increase in the content of fat, protein, total dry matter and casein together with a significant decrease in the content of lactose in the late stage of lactation. There was also a significant increase confirmed for the concentration of urea and the freezing point in milk along with the lactation progress. Depending on the stage of lactation, milk yield in Lacaune sheep significantly decreased as lactation progressed. Analysis of the fatty acid composition in milk of Lacaune sheep proved a significant decrease in the concentrations of C4 : 0, C6 : 0, C11 : 0, C12 : 0, C13 : 0, C15 : 0, C17 : 1, C18 : 2n6 and C18 : 3n6 as well as the n6 concentrations and the n6 / n3 ratio. The opposite trend was observed for concentrations of C10 : 0, C14 : 1, C16 : 0, C16 : 1, C18 : 0, C20 : 2, C18 : 3n3, C20 : 3n6, C20 : 5n3 and C22 : 6n3 as well as for the n3 concentrations. When compared to the early lactation stage, the C18 : 3n6 and n6 concentrations were significantly lower in the late lactation stage, while the C20 : 2 and C20 : 5n3 concentrations were significantly lower in the middle lactation stage when compared to the late lactation stage. There were many significant positive and negative correlations determined between the researched properties of milk. The research results obtained with Lacaune sheep's milk can be compared to the results of other studies, except for the lower content of milk fat. This confirms the good adaptability of Lacaune sheep to different breeding conditions and the necessity to provide sheep with quality pastures for grazing.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139809909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammed A. Kamal, Mahmoud A. Khalf, Zakia A. M. Ahmed, Jakeen A. Eljakee, R. Alhotan, Mohammed A. Al-Badwi, E. O. Hussein, B. Gálik, A. A. Saleh
Abstract. Poor-quality drinking water plays a detrimental role in the suppression of calf immunity, giving rise to an increased rate of calf mortality. The present study aims to evaluate the causes of calf mortality in beef and dairy farms in relation to drinking water quality (DWQ). A convenience sample of 132 Egyptian cattle farms suffering from emerging epidemics was surveyed by collecting drinking water samples for physicochemical and microbial analysis and using a questionnaire to record hygienic risk factors affecting calf health. Statistical analysis correlates water parameters with rates of calf diarrhea, respiratory problems, severe depression, sudden death and mortality. High percentages of water sample quality parameters, e.g. pH, total dissolved solids (TDSs), hardness, chloride, nitrate, sulfate, total colony count (TCC) and total coliform count (TCFC), are above permissible limits. Water parameters, except pH, show a significant moderate positive correlation with causes of calf mortality (ρ 0.331–0.66) in winter and summer. Each cause of calf mortality was predicted by a specific water parameter, and the water nitrate level was the highest predictor, with the highest values (β = 0.504–0.577), followed by the water TDS, sulfate and microbial levels. Weak to moderate correlation (ρ 0.151–0.367) was found between calf mortality causes and some hygienic risk factors such as operation type, calf housing, calf feeders, bedding type, water source, water pipe type, drinker lining and wheel dipping. We could conclude that DWQ greatly affects causes of calf mortality, but we cannot exclude some farm hygienic risk factors.
{"title":"Effect of water quality on causes of calf mortality in cattle-farm-associated epidemics","authors":"Mohammed A. Kamal, Mahmoud A. Khalf, Zakia A. M. Ahmed, Jakeen A. Eljakee, R. Alhotan, Mohammed A. Al-Badwi, E. O. Hussein, B. Gálik, A. A. Saleh","doi":"10.5194/aab-67-25-2024","DOIUrl":"https://doi.org/10.5194/aab-67-25-2024","url":null,"abstract":"Abstract. Poor-quality drinking water plays a detrimental role in the suppression of calf immunity, giving rise to an increased rate of calf mortality. The present study aims to evaluate the causes of calf mortality in beef and dairy farms in relation to drinking water quality (DWQ). A convenience sample of 132 Egyptian cattle farms suffering from emerging epidemics was surveyed by collecting drinking water samples for physicochemical and microbial analysis and using a questionnaire to record hygienic risk factors affecting calf health. Statistical analysis correlates water parameters with rates of calf diarrhea, respiratory problems, severe depression, sudden death and mortality. High percentages of water sample quality parameters, e.g. pH, total dissolved solids (TDSs), hardness, chloride, nitrate, sulfate, total colony count (TCC) and total coliform count (TCFC), are above permissible limits. Water parameters, except pH, show a significant moderate positive correlation with causes of calf mortality (ρ 0.331–0.66) in winter and summer. Each cause of calf mortality was predicted by a specific water parameter, and the water nitrate level was the highest predictor, with the highest values (β = 0.504–0.577), followed by the water TDS, sulfate and microbial levels. Weak to moderate correlation (ρ 0.151–0.367) was found between calf mortality causes and some hygienic risk factors such as operation type, calf housing, calf feeders, bedding type, water source, water pipe type, drinker lining and wheel dipping. We could conclude that DWQ greatly affects causes of calf mortality, but we cannot exclude some farm hygienic risk factors.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"39 40","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139527959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
David Zapletal, Radka Dobšíková, V. Šimek, Josef Kameník, František Ježek
Abstract. The present study was conducted to determine the effect of dietary inclusion of the wormwood (Artemisia absinthium L.) meal on growth performance, carcass composition, physico-chemical traits and amino acid profile in meats of fattened broilers. In a completely randomised block design, a total of 288 female broilers that were 21 d old (Ross 308) were divided into four dietary groups and fed for 3 weeks: the control basal broiler diet (C), without any anticoccidial or wormwood herb (WH) supplementation, and the C diet plus 10 g (WW1 group), 50 g (WW5 group) or 100 g (WW10 group) of WH meal supplementation per kilogram of basal diet. At the end of the experiment (day 42), broilers were randomly selected for carcass composition and meat quality trait evaluation. In conclusion, the final live weight of chickens was not affected by diets with higher WH levels (P>0.05). For the entire experimental period, the feed conversion ratio raised with an increasing WH level in diets, showing the highest value in chickens of the WW10 group (P<0.01). Dietary supplementation with wormwood (WW) had no negative effects on the carcass composition or on the chemical and physical traits of meat quality assessed. By contrast, it can be assumed that WH dietary supplementation influenced, predominantly, proteosynthesis of chickens, resulting in alteration of amino acid profiles in meats, where especially increasing aspartic acid and valine contents (P<0.001) in the leg meat were found. Our findings indicate that the supplementation of 5 % WH to the diet showed favourable results for chicken performance. However, it is necessary to conduct further studies dealing with WH dietary effects on metabolism and heath control in chickens.
{"title":"Growth performance, carcass composition, physico-chemical traits and amino acid profile of meat depending on wormwood (Artemisia absinthium L.) dietary supplementation in broilers","authors":"David Zapletal, Radka Dobšíková, V. Šimek, Josef Kameník, František Ježek","doi":"10.5194/aab-67-1-2024","DOIUrl":"https://doi.org/10.5194/aab-67-1-2024","url":null,"abstract":"Abstract. The present study was conducted to determine the effect of dietary inclusion of the wormwood (Artemisia absinthium L.) meal on growth performance, carcass composition, physico-chemical traits and amino acid profile in meats of fattened broilers. In a completely randomised block design, a total of 288 female broilers that were 21 d old (Ross 308) were divided into four dietary groups and fed for 3 weeks: the control basal broiler diet (C), without any anticoccidial or wormwood herb (WH) supplementation, and the C diet plus 10 g (WW1 group), 50 g (WW5 group) or 100 g (WW10 group) of WH meal supplementation per kilogram of basal diet. At the end of the experiment (day 42), broilers were randomly selected for carcass composition and meat quality trait evaluation. In conclusion, the final live weight of chickens was not affected by diets with higher WH levels (P>0.05). For the entire experimental period, the feed conversion ratio raised with an increasing WH level in diets, showing the highest value in chickens of the WW10 group (P<0.01). Dietary supplementation with wormwood (WW) had no negative effects on the carcass composition or on the chemical and physical traits of meat quality assessed. By contrast, it can be assumed that WH dietary supplementation influenced, predominantly, proteosynthesis of chickens, resulting in alteration of amino acid profiles in meats, where especially increasing aspartic acid and valine contents (P<0.001) in the leg meat were found. Our findings indicate that the supplementation of 5 % WH to the diet showed favourable results for chicken performance. However, it is necessary to conduct further studies dealing with WH dietary effects on metabolism and heath control in chickens.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammed H Alzawqari, Mustafa Shukry Atta, A. Metwally, S. Selim, Mohammad A. M. Wadaan, I. Kim, Sungbo Cho, Hossam M. Eltahan, Mahmoud Alagawany, R. Alhotan, Ali R. Al Sulaiman, E. O. Hussein, Ahmed A. Saleh
Abstract. This study aimed to show the effect of bile acid (BA) and xylanase (Xyl) supplementation on the growth, fat digestibility, serum lipid metabolites, and ileal digesta viscosity of broilers. A total of 720 1 d old male broilers were allocated to one of nine treatments with four replicates in each under a factorial design arrangement of three levels of BA (0 %, 0.25 %, and 0.50 %) and three levels of Xyl (0 %, 0.05 %, and 0.10 %) supplementation. The duration of the experiment was 35 d (7–42 d). Growth performance, blood lipids, fat digestibility, and ileal digesta viscosity were determined. The experimental treatments did not affect feed intake (FI) and weight gain (WG). Supplementation of BA or Xyl did not significantly ameliorate the feed conversion rate (FCR) (p<0.05). The addition of BA linearly increased fat digestibility. At 7–21 d of age, the addition of BA or Xyl had a significant (p<0.05) increase in serum cholesterol (Chol) but no significant difference for other serum lipid parameters in broiler chickens fed with Xyl in the starter and grower periods. However, the supplementation of 0.5 % BA at 7–21 d of age significantly increased the Chol and low-density-lipoprotein (LDL) levels. The results of this trial revealed that the supplementation of xylanases had a great effect on the degradation of arabinoxylan from wheat, which led to a relatively greater reduction in ileal digesta viscosity; it was also found that supplementation of BA significantly increased the concentration of serum lipid metabolites, whereas BA and Xyl supplementation linearly increased the fat digestibility of the birds fed wheat and tallow diets.
摘要本研究旨在说明胆汁酸（BA）和木聚糖酶（Xyl）对肉鸡生长、脂肪消化率、血清脂质代谢物和回肠消化液粘度的影响。将720只1日龄雄性肉鸡分配到9个处理中的一个处理，每个处理有4个重复，采用因子设计法，分别添加3个水平的BA（0 %、0.25 %和0.50 %）和3个水平的Xyl（0 %、0.05 %和0.10 %）。实验持续时间为 35 天（7-42 天）。测定了生长性能、血脂、脂肪消化率和回肠消化液粘度。实验处理对采食量（FI）和增重（WG）没有影响。添加 BA 或 Xyl 并未显著改善饲料转化率（FCR）（p<0.05）。添加 BA 可线性提高脂肪消化率。7-21日龄时，添加BA或Xyl会显著增加肉鸡血清胆固醇（Chol）（p<0.05），但在开产期和生长期添加Xyl的肉鸡血清其他脂质参数没有显著差异。然而，在 7-21 日龄添加 0.5 % 的 BA 可显著提高胆固醇和低密度脂蛋白（LDL）水平。试验结果表明，添加木聚糖酶对小麦中阿拉伯木聚糖的降解有很大的影响，从而使回肠消化液粘度相对降低；试验还发现，添加 BA 能显著提高血清脂质代谢物的浓度，而添加 BA 和 Xyl 能线性提高饲喂小麦和牛油日粮的禽类的脂肪消化率。
{"title":"Growth performance, blood lipids, and fat digestibility of broilers fed diets supplemented with bile acid and xylanase","authors":"Mohammed H Alzawqari, Mustafa Shukry Atta, A. Metwally, S. Selim, Mohammad A. M. Wadaan, I. Kim, Sungbo Cho, Hossam M. Eltahan, Mahmoud Alagawany, R. Alhotan, Ali R. Al Sulaiman, E. O. Hussein, Ahmed A. Saleh","doi":"10.5194/aab-66-451-2023","DOIUrl":"https://doi.org/10.5194/aab-66-451-2023","url":null,"abstract":"Abstract. This study aimed to show the effect of bile acid (BA) and xylanase (Xyl) supplementation on the growth, fat digestibility, serum lipid metabolites, and ileal digesta viscosity of broilers. A total of 720 1 d old male broilers were allocated to one of nine treatments with four replicates in each under a factorial design arrangement of three levels of BA (0 %, 0.25 %, and 0.50 %) and three levels of Xyl (0 %, 0.05 %, and 0.10 %) supplementation. The duration of the experiment was 35 d (7–42 d). Growth performance, blood lipids, fat digestibility, and ileal digesta viscosity were determined. The experimental treatments did not affect feed intake (FI) and weight gain (WG). Supplementation of BA or Xyl did not significantly ameliorate the feed conversion rate (FCR) (p<0.05). The addition of BA linearly increased fat digestibility. At 7–21 d of age, the addition of BA or Xyl had a significant (p<0.05) increase in serum cholesterol (Chol) but no significant difference for other serum lipid parameters in broiler chickens fed with Xyl in the starter and grower periods. However, the supplementation of 0.5 % BA at 7–21 d of age significantly increased the Chol and low-density-lipoprotein (LDL) levels. The results of this trial revealed that the supplementation of xylanases had a great effect on the degradation of arabinoxylan from wheat, which led to a relatively greater reduction in ileal digesta viscosity; it was also found that supplementation of BA significantly increased the concentration of serum lipid metabolites, whereas BA and Xyl supplementation linearly increased the fat digestibility of the birds fed wheat and tallow diets.","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"49 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139167021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. The skeletal muscles of mammals are composed of fibers of different morphological, metabolic and functional characteristics. The properties of muscle fibers may be determined genetically as well as by environmental factors such as the age of the animals, their physical activity, the level of nutrition, or the selection intensity. The present study was conducted to determine the influence of genotype (Polish Lowland vs. Polish Heath) and body weight at slaughter (23–25 kg vs. 35–40 kg) of lambs on histological characteristics of muscle fibers in musculus longissimus lumborum (LL) and musculus semitendinosus (ST) skeletal muscles and their impact on chosen meat quality features. Three types of muscle fibers were identified: slow-twitch oxidative (STO), fast-twitch oxidative (FTO) and fast-twitch glycolytic (FTG). Differences in the diameters of individual fiber types between the LL and ST muscles have been found in both investigated genotypes. The diameters of the analyzed types of fibers were usually larger in the ST muscle compared to the LL muscle. The lambs of the more primitive Polish Heath breed were characterized by a smaller diameter of all fiber types, especially in the LL muscle. The higher proportion of STO fibers and the lower proportion of FTO fibers have been found in the LL muscle of Polish Heath lambs in the group with lower body weight. The breed of lambs, muscle type and slaughter body weight had an impact on some meat quality characteristics, especially color, intramuscular fat content and expressed juice.
{"title":"The effect of breed and body weight at slaughter on histochemical muscle fiber characteristics and meat quality of longissimus lumborum and semitendinosus lamb muscles","authors":"W. Rant, A. Radzik-Rant","doi":"10.5194/aab-66-439-2023","DOIUrl":"https://doi.org/10.5194/aab-66-439-2023","url":null,"abstract":"Abstract. The skeletal muscles of mammals are composed of fibers of different morphological, metabolic and functional characteristics. The properties of muscle fibers may be determined genetically as well as by environmental factors such as the age of the animals, their physical activity, the level of nutrition, or the selection intensity. The present study was conducted to determine the influence of genotype (Polish Lowland vs. Polish Heath) and body weight at slaughter (23–25 kg vs. 35–40 kg) of lambs on histological characteristics of muscle fibers in musculus longissimus lumborum (LL) and musculus semitendinosus (ST) skeletal muscles and their impact on chosen meat quality features. Three types of muscle fibers were identified: slow-twitch oxidative (STO), fast-twitch oxidative (FTO) and fast-twitch glycolytic (FTG). Differences in the diameters of individual fiber types between the LL and ST muscles have been found in both investigated genotypes. The diameters of the analyzed types of fibers were usually larger in the ST muscle compared to the LL muscle. The lambs of the more primitive Polish Heath breed were characterized by a smaller diameter of all fiber types, especially in the LL muscle. The higher proportion of STO fibers and the lower proportion of FTO fibers have been found in the LL muscle of Polish Heath lambs in the group with lower body weight. The breed of lambs, muscle type and slaughter body weight had an impact on some meat quality characteristics, especially color, intramuscular fat content and expressed juice.","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"228 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139180923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hashimoto, M. Taniguchi, Ayane Edo, Tetsushi Ono, Tetty Barunawati Siagian, Hiroaki Sekine, M. Nagahara, T. Otoi, Mitsuhiro Takagi
Abstract. This study investigated the relationship between oxidation and antioxidation parameters before superovulation (SOV) treatment and embryo recovery in donor cows. The relative redox status of the 61 donor cows was evaluated based on the median values of diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) measurements (d-ROMs of 100 U.CARR; BAP of 2413 µmol L−1) before SOV treatment. Following this, the animals were divided into four groups: cows with low d-ROMs but high BAP were assigned to the “LH” group (n=11), cows with high d-ROMs and BAP were assigned to the “HH” group (n=20), cows with high d-ROMs but low BAP were assigned to the “HL” group (n=10), and cows with low d-ROMs and BAP were assigned to the “LL” group (n=20). Embryos were collected from superovulated cows 6 d after artificial insemination. The percentages of fertilised, transferable, and Code-1 embryos were significantly higher (P<0.05) in the HH group than those in the HL group. The HH group had the highest mean values for all embryo recovery results. Our results suggest that the redox status of donor cows before SOV treatment affects embryo recovery, as cows with high levels of both oxidative and antioxidative status have better embryo production.
{"title":"Impact of redox status of donor cows before superovulation treatment on in vivo embryo production","authors":"S. Hashimoto, M. Taniguchi, Ayane Edo, Tetsushi Ono, Tetty Barunawati Siagian, Hiroaki Sekine, M. Nagahara, T. Otoi, Mitsuhiro Takagi","doi":"10.5194/aab-66-433-2023","DOIUrl":"https://doi.org/10.5194/aab-66-433-2023","url":null,"abstract":"Abstract. This study investigated the relationship between oxidation and antioxidation parameters before superovulation (SOV) treatment and embryo recovery in donor cows. The relative redox status of the 61 donor cows was evaluated based on the median values of diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) measurements (d-ROMs of 100 U.CARR; BAP of 2413 µmol L−1) before SOV treatment. Following this, the animals were divided into four groups: cows with low d-ROMs but high BAP were assigned to the “LH” group (n=11), cows with high d-ROMs and BAP were assigned to the “HH” group (n=20), cows with high d-ROMs but low BAP were assigned to the “HL” group (n=10), and cows with low d-ROMs and BAP were assigned to the “LL” group (n=20). Embryos were collected from superovulated cows 6 d after artificial insemination. The percentages of fertilised, transferable, and Code-1 embryos were significantly higher (P<0.05) in the HH group than those in the HL group. The HH group had the highest mean values for all embryo recovery results. Our results suggest that the redox status of donor cows before SOV treatment affects embryo recovery, as cows with high levels of both oxidative and antioxidative status have better embryo production.","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139182466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Elkomy, A. El-Saadany, E. Y. Shreif, Amal A. Bayoumi, Marwa H. Abd El-Maged, M. Alagawany, Ahmed A. Saleh, Sungbo Cho, I. Kim, Hossam M. Eltahan
Abstract. This trial was performed to determine the effect of anise (Ans) and grape seed (Grp) oil inclusion in diets of Japanese quail on performance, carcasses, blood parameters, antioxidant activity, and hematological blood structure. The 35 d feeding trial was conducted on two hundred 7 d old Japanese quails, allocated to four treatment groups with five replicates each. Ans and Grp were examined at different inclusion levels: 0 % (control), Ans 0.5 %, Grp 0.5 %, and Ans 0.25 % + Grp 0.25 %, in a completely randomized design. The results showed that at the end of the trial (42 d), the oil supplementation had positive effects (P<0.001) on the productivity parameters, while feed intake (FI) did not differ from the control group. In addition, oil supplementation linearly improved (P<0.05) the dressing percentage, carcass yield, and immune organs' relative weights, while it decreased (p<0.01) the abdominal fat yield. Plasma total protein (TP), albumin (Alb), and globulin (Glob) were significantly increased in the Grp group. Despite total plasma cholesterol not being significantly affected by treatments, Ans and Grp essential oils decreased low-density lipoprotein cholesterol (LDL) and increased high-density lipoprotein cholesterol (HDL). Moreover, total antioxidant capacity (TAC) concentration was increased (P≤0.001) in the treated groups, while malondialdehyde (MDA) decreased (P≤0.001). Results of a caecal bacterial count revealed that Ans and its combination with Grp led to an increase (P≤0.001) in Lactobacillus spp. count. On the other hand, Ans, Grp, and their combination led to a significant reduction (P≤0.001) in E. coli spp. and Salmonella spp. compared to the control group. It is concluded that Ans and Grp seed oils could be used as valuable essential oils in quails' diets to improve the performance and immune response, enhance the antioxidant activity, and reduce caecal pathogenic microbes.
{"title":"Anise and grape seed oils as a feed additive to improve the performance, immune response, and antioxidant activity and reduce caecal pathogenic microbes of quail","authors":"A. Elkomy, A. El-Saadany, E. Y. Shreif, Amal A. Bayoumi, Marwa H. Abd El-Maged, M. Alagawany, Ahmed A. Saleh, Sungbo Cho, I. Kim, Hossam M. Eltahan","doi":"10.5194/aab-66-379-2023","DOIUrl":"https://doi.org/10.5194/aab-66-379-2023","url":null,"abstract":"Abstract. This trial was performed to determine the effect of anise (Ans) and grape seed (Grp) oil inclusion in diets of Japanese quail on performance, carcasses, blood parameters, antioxidant activity, and hematological blood structure. The 35 d feeding trial was conducted on two hundred 7 d old Japanese quails, allocated to four treatment groups with five replicates each. Ans and Grp were examined at different inclusion levels: 0 % (control), Ans 0.5 %, Grp 0.5 %, and Ans 0.25 % + Grp 0.25 %, in a completely randomized design. The results showed that at the end of the trial (42 d), the oil supplementation had positive effects (P<0.001) on the productivity parameters, while feed intake (FI) did not differ from the control group. In addition, oil supplementation linearly improved (P<0.05) the dressing percentage, carcass yield, and immune organs' relative weights, while it decreased (p<0.01) the abdominal fat yield. Plasma total protein (TP), albumin (Alb), and globulin (Glob) were significantly increased in the Grp group. Despite total plasma cholesterol not being significantly affected by treatments, Ans and Grp essential oils decreased low-density lipoprotein cholesterol (LDL) and increased high-density lipoprotein cholesterol (HDL). Moreover, total antioxidant capacity (TAC) concentration was increased (P≤0.001) in the treated groups, while malondialdehyde (MDA) decreased (P≤0.001). Results of a caecal bacterial count revealed that Ans and its combination with Grp led to an increase (P≤0.001) in Lactobacillus spp. count. On the other hand, Ans, Grp, and their combination led to a significant reduction (P≤0.001) in E. coli spp. and Salmonella spp. compared to the control group. It is concluded that Ans and Grp seed oils could be used as valuable essential oils in quails' diets to improve the performance and immune response, enhance the antioxidant activity, and reduce caecal pathogenic microbes.","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"175 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139217350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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