Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.287.2023
Yuan Zhang
In this paper, the mechanical, dry shrinkage and frost resistance performance of cement stabilized aggregate materials were investigated. Furthermore, the nonlinear analysis between dry shrinkage strain and frost resistance performance was conducted. The results showed that the mechanical performance of cement stabilized aggregate with different cement content showed a linear improving relationship. A higher cement content owns a correspondingly better mechanical performance. With the different moisture content, the dry shrinkage and frost resistance performance of cement stabilized aggregate material showed a similar improving and deteriorating trend. With the increasing moisture content, the dry shrinkage strain and dry shrinkage coefficient were improved and reached to the optimum values, and then started to deteriorate. The optimum dry shrinkage and frost resistance performance were obtained with 6.0% moisture content. The nonlinear relationship between dry shrinkage strain and frost resistance coefficient was gained and the correspondingly optimum dry shrinkage strain and frost resistance was discerned, i.e. 94.3×10-6 and 47.6, respectively. The moisture content significantly influence the pore parameters of prepared samples, whose trend followed those of dry shrinkage and frost resistance performance. The pore size distribution of these composites shifted toward smaller pore size scope with a proper moisture content. In addition, scanning electron micrographs (SEM) showed that the denser microstructure of prepared cement stabilized aggregate materials.
{"title":"Dry Shrinkage and Frost Resistance Performance of Cement Stabilized Aggregate Materials","authors":"Yuan Zhang","doi":"10.56028/aetr.8.1.287.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.287.2023","url":null,"abstract":"In this paper, the mechanical, dry shrinkage and frost resistance performance of cement stabilized aggregate materials were investigated. Furthermore, the nonlinear analysis between dry shrinkage strain and frost resistance performance was conducted. The results showed that the mechanical performance of cement stabilized aggregate with different cement content showed a linear improving relationship. A higher cement content owns a correspondingly better mechanical performance. With the different moisture content, the dry shrinkage and frost resistance performance of cement stabilized aggregate material showed a similar improving and deteriorating trend. With the increasing moisture content, the dry shrinkage strain and dry shrinkage coefficient were improved and reached to the optimum values, and then started to deteriorate. The optimum dry shrinkage and frost resistance performance were obtained with 6.0% moisture content. The nonlinear relationship between dry shrinkage strain and frost resistance coefficient was gained and the correspondingly optimum dry shrinkage strain and frost resistance was discerned, i.e. 94.3×10-6 and 47.6, respectively. The moisture content significantly influence the pore parameters of prepared samples, whose trend followed those of dry shrinkage and frost resistance performance. The pore size distribution of these composites shifted toward smaller pore size scope with a proper moisture content. In addition, scanning electron micrographs (SEM) showed that the denser microstructure of prepared cement stabilized aggregate materials.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139322052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.294.2023
Meng Zhang, Zhong Fang, Yu Hao, Wei Du, Xuchao Pan, Jun-jie Jiao, Yong He
The EMP couples with electronic devices via cables, inducing electromagnetic damage effects. This paper elucidates the operational principles of a navigation receiver and simplifies the study by focusing on the radio frequency front-end module. Many PCI tests were conducted on its signal input port, yielding the damage threshold of the navigation receiver. An analysis of the output waveforms of the modules that incurred damage was also performed. Through infrared research, it was discovered that the damaged LNA experiences a rapid temperature rise to around 73°C during operation with applied power. The electromagnetic damage effect in the LNA primarily stems from internal MOS transistor short circuits. The BPF protects the subsequent circuit stages but is also susceptible to damage.
{"title":"Research on Electromagnetic Damage Effects in Navigation Receiver by PCI Testing","authors":"Meng Zhang, Zhong Fang, Yu Hao, Wei Du, Xuchao Pan, Jun-jie Jiao, Yong He","doi":"10.56028/aetr.8.1.294.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.294.2023","url":null,"abstract":"The EMP couples with electronic devices via cables, inducing electromagnetic damage effects. This paper elucidates the operational principles of a navigation receiver and simplifies the study by focusing on the radio frequency front-end module. Many PCI tests were conducted on its signal input port, yielding the damage threshold of the navigation receiver. An analysis of the output waveforms of the modules that incurred damage was also performed. Through infrared research, it was discovered that the damaged LNA experiences a rapid temperature rise to around 73°C during operation with applied power. The electromagnetic damage effect in the LNA primarily stems from internal MOS transistor short circuits. The BPF protects the subsequent circuit stages but is also susceptible to damage.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.236.2023
Ruichuan Li, Lanzheng Chen, Defa Li, Lin Wang, Zhengyu Li, Peng Zhang
In response to the problem of outdated traditional PID control performance and excessively single control parameters in automotive suspension, a two level fuzzy PID control system for automotive semi-active suspension is proposed. The system has two control loops: the first level control loop is PID control, and the second level control loop is fuzzy control. The second level control loop fully utilizes the motion state of unsprung mass that affects the comprehensive performance of the suspension to achieve fine adjustments to the PID controller control parameters in the first level control loop, making the PID controller control parameters more suitable for adjusting suspension performance after hierarchical adjustment, thereby achieving the goal of improving ride comfort. The analysis data shows that the control performance of the two level fuzzy PID control system is significantly better than that of the PID control system, and the comprehensive performance of the suspension and ride comfort has been significantly improved.
{"title":"Design and Research of a Two Level Fuzzy PID Electronic Control System for Automotive Semi-Active Suspension","authors":"Ruichuan Li, Lanzheng Chen, Defa Li, Lin Wang, Zhengyu Li, Peng Zhang","doi":"10.56028/aetr.8.1.236.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.236.2023","url":null,"abstract":"In response to the problem of outdated traditional PID control performance and excessively single control parameters in automotive suspension, a two level fuzzy PID control system for automotive semi-active suspension is proposed. The system has two control loops: the first level control loop is PID control, and the second level control loop is fuzzy control. The second level control loop fully utilizes the motion state of unsprung mass that affects the comprehensive performance of the suspension to achieve fine adjustments to the PID controller control parameters in the first level control loop, making the PID controller control parameters more suitable for adjusting suspension performance after hierarchical adjustment, thereby achieving the goal of improving ride comfort. The analysis data shows that the control performance of the two level fuzzy PID control system is significantly better than that of the PID control system, and the comprehensive performance of the suspension and ride comfort has been significantly improved.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139322023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.182.2023
Yanzhi Tang, Xiaofang Duan
By adding ordinary glass fiber and alkali resistant glass fiber into concrete, the development of axial compressive strength, axial tensile strength and elastic modulus of concrete are studied. The results show that with the increase of glass fiber, the axial compressive strength increases first and then decreases; the axial tensile strength increases; the elastic modulus decreases. The results show that glass fiber can improve the strength of concrete, and reduce the elastic modulus.
{"title":"Experimental research on mechanical properties and elastic modulus of glass fiber reinforced concrete","authors":"Yanzhi Tang, Xiaofang Duan","doi":"10.56028/aetr.8.1.182.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.182.2023","url":null,"abstract":"By adding ordinary glass fiber and alkali resistant glass fiber into concrete, the development of axial compressive strength, axial tensile strength and elastic modulus of concrete are studied. The results show that with the increase of glass fiber, the axial compressive strength increases first and then decreases; the axial tensile strength increases; the elastic modulus decreases. The results show that glass fiber can improve the strength of concrete, and reduce the elastic modulus.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taking the steel canopy project of the main stage of Chengdu Open-air Music Square as the research object, the project analyzes the construction difficulty, tight schedule and high quality requirements of the steel canopy from the aspects of construction difficulties, installation methods and construction process; puts forward reasonable structure division and construction steps; at the same time, the project innovatively puts forward the large-scale inverted "7 At the same time, the project innovatively proposes a large inverted "7" truss structure lifting method and an anti-deformation structure and its lifting method for the lifting of hyperbolic mesh shell structure; and fully utilizes construction simulation analysis, monitoring and testing technology, etc., to ensure the safety and quality of the construction of the steel canopy. The results show that the overall deformation of the steel canopy structure after molding is small, the construction efficiency has been improved by 38%, and all the nodes docked with the curtain wall project are within the installation design allowable values. The research results provide technical references for the application of similar canopy structure construction in China in the future.
{"title":"Research on Key Technologies for Construction of Steel Canopy Steel Structure in Chengdu Open Air Music Square","authors":"Dalin Zhang, Xiang Gao, Yongdong Wang, Haichuan Zhang","doi":"10.56028/aetr.8.1.188.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.188.2023","url":null,"abstract":"Taking the steel canopy project of the main stage of Chengdu Open-air Music Square as the research object, the project analyzes the construction difficulty, tight schedule and high quality requirements of the steel canopy from the aspects of construction difficulties, installation methods and construction process; puts forward reasonable structure division and construction steps; at the same time, the project innovatively puts forward the large-scale inverted \"7 At the same time, the project innovatively proposes a large inverted \"7\" truss structure lifting method and an anti-deformation structure and its lifting method for the lifting of hyperbolic mesh shell structure; and fully utilizes construction simulation analysis, monitoring and testing technology, etc., to ensure the safety and quality of the construction of the steel canopy. The results show that the overall deformation of the steel canopy structure after molding is small, the construction efficiency has been improved by 38%, and all the nodes docked with the curtain wall project are within the installation design allowable values. The research results provide technical references for the application of similar canopy structure construction in China in the future.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139322028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.264.2023
Jianmei Zhou
Two kinds of inorganic reinforcing materials, single Portland cement clinker, compound Portland cement clinker and mineral powder, were used to analyze the effects of desulfurized gypsum on the properties of desulfurized gypsum based cementation material. The results show that either single Portland cement clinker or compound Portland cement clinker and mineral powder can effectively improve the mechanical properties and softening coefficient of the material, and the effect of compound mixing is better. The optimum dosage of Portland cement clinker is 10%, the 7d and 28d bending strength of cementing material is 2.5MPa and 4.1MPa, the compressive strength is 4.6MPa and 9.8MPa, and the softening coefficient is 0.61. The appropriate composite content of Portland cement clinker and mineral powder is: Portland cement clinker 5%~10%, mineral powder 10%~15%, gelling material 7d, 28d bending strength of 3.0~3.5MPa, 6.3~7.4MPa, compressive strength of 5.4~6.7MPa, 12.1~13.6MPa, softening coefficient of 0.58~0.62.
{"title":"Study on improving properties of desulfurized gypsum based cementitious materials","authors":"Jianmei Zhou","doi":"10.56028/aetr.8.1.264.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.264.2023","url":null,"abstract":"Two kinds of inorganic reinforcing materials, single Portland cement clinker, compound Portland cement clinker and mineral powder, were used to analyze the effects of desulfurized gypsum on the properties of desulfurized gypsum based cementation material. The results show that either single Portland cement clinker or compound Portland cement clinker and mineral powder can effectively improve the mechanical properties and softening coefficient of the material, and the effect of compound mixing is better. The optimum dosage of Portland cement clinker is 10%, the 7d and 28d bending strength of cementing material is 2.5MPa and 4.1MPa, the compressive strength is 4.6MPa and 9.8MPa, and the softening coefficient is 0.61. The appropriate composite content of Portland cement clinker and mineral powder is: Portland cement clinker 5%~10%, mineral powder 10%~15%, gelling material 7d, 28d bending strength of 3.0~3.5MPa, 6.3~7.4MPa, compressive strength of 5.4~6.7MPa, 12.1~13.6MPa, softening coefficient of 0.58~0.62.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139322046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.300.2023
Weirong Li, Kai Shen, Yanfang Li, Dong Pang, Fangfei Liu, Huimin Zhou, Lingfei Kong, Xuqiang Huang, Di Tie
Flow velocity as the basic hydrodynamic condition significantly affects bacterial proliferation and biofilm formation, yet how different flow velocity influences the attachment and distribution of dominant bacteria in different ecological types is poorly understood. In this work, We collected samples from the bottom, side walls and water samples at the flow rates of 0.794m/s and 0.538m/s, respectively. Through high-throughput sequencing identification and analysis technology, it was found that the composition abundance of Acinetobacter and Psychrobacter, as dominant bacteria, could reach 70%-80% in the floating state, and the attached bacteria at low flow rates were about 20% higher than those at high flow rates. The results showed that the high flow rate inhibited the attachment behavior of dominant bacteria and changed the species composition structure in the overlapping area of multiple samples, which provided a new direction for the prevention and control of microbial corrosion and pollution in waters.
{"title":"Distribution mechanism of dominant genera and species of bacteria under different river velocity","authors":"Weirong Li, Kai Shen, Yanfang Li, Dong Pang, Fangfei Liu, Huimin Zhou, Lingfei Kong, Xuqiang Huang, Di Tie","doi":"10.56028/aetr.8.1.300.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.300.2023","url":null,"abstract":"Flow velocity as the basic hydrodynamic condition significantly affects bacterial proliferation and biofilm formation, yet how different flow velocity influences the attachment and distribution of dominant bacteria in different ecological types is poorly understood. In this work, We collected samples from the bottom, side walls and water samples at the flow rates of 0.794m/s and 0.538m/s, respectively. Through high-throughput sequencing identification and analysis technology, it was found that the composition abundance of Acinetobacter and Psychrobacter, as dominant bacteria, could reach 70%-80% in the floating state, and the attached bacteria at low flow rates were about 20% higher than those at high flow rates. The results showed that the high flow rate inhibited the attachment behavior of dominant bacteria and changed the species composition structure in the overlapping area of multiple samples, which provided a new direction for the prevention and control of microbial corrosion and pollution in waters.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.215.2023
Mengxuan Lv, Bo Yuan, Haitao Chen, Chenglong Zhang
Recently, the Statistical Communiqué of the People’s Republic of China on the 2022 National Economic and Social Development (hereinafter referred to as "Communiqués") was published by the National Bureau of Statistics. According to the Communiqués, China’s total primary energy consumption in 2022 amounted to 5.41 billion tons of standard coal. It is projected that by 2025, the total energy consumption will reach approximately 5.84-6.04 billion tons of standard coal. In order to meet the target of non-fossil energy consumption accounting for 20% of total energy consumption by 2025, the installed new energy capacity within operating areas of State Grid Corporation of China is anticipated to exceed the previous estimate by 100-140 million kilowatts. From a risk assessment standpoint, the research team has constructed an evaluation model to gauge China’s new energy development trend, taking into account the accomplishment of the consumption proportion target for non-fossil energy. They delve into the issues brought about by the unexpected development of new energy and put forth pertinent measures to tackle them.
{"title":"Study on China’s New Energy Development Trend through Achieving the Consumption Proportion Target for Non-fossil Energy","authors":"Mengxuan Lv, Bo Yuan, Haitao Chen, Chenglong Zhang","doi":"10.56028/aetr.8.1.215.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.215.2023","url":null,"abstract":"Recently, the Statistical Communiqué of the People’s Republic of China on the 2022 National Economic and Social Development (hereinafter referred to as \"Communiqués\") was published by the National Bureau of Statistics. According to the Communiqués, China’s total primary energy consumption in 2022 amounted to 5.41 billion tons of standard coal. It is projected that by 2025, the total energy consumption will reach approximately 5.84-6.04 billion tons of standard coal. In order to meet the target of non-fossil energy consumption accounting for 20% of total energy consumption by 2025, the installed new energy capacity within operating areas of State Grid Corporation of China is anticipated to exceed the previous estimate by 100-140 million kilowatts. From a risk assessment standpoint, the research team has constructed an evaluation model to gauge China’s new energy development trend, taking into account the accomplishment of the consumption proportion target for non-fossil energy. They delve into the issues brought about by the unexpected development of new energy and put forth pertinent measures to tackle them.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.259.2023
Jianmei Zhou
In the process of preparing α-hemihydrous gypsum from desulfurized gypsum under atmospheric pressure, the optimum conditions of crystallization transformation and modification of desulfurized gypsum were studied. The effects of the type and concentration of composite salts (CaCl2, KCl, MgCl2 mixed solution) on the crystallization and modification of desulfurized gypsum were studied. The results were further verified by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC/TGA), so as to determine the ideal directional crystallization control index. The experimental results show that temperature, type and concentration of salt solution are the most sensitive factors in the crystallization process of desulfurized gypsum. The optimal control conditions for obtaining α-hemihydrous gypsum with good crystalline form are temperature 95℃~98℃, concentration of composite salt solution (CaCl2: MgCl2: KCl = 25:5:1) is 25%~30%, and slurry concentration is greater than 20%.
{"title":"Experimental study on preparation of α-hemihydrous gypsum by modification of desulfurized gypsum","authors":"Jianmei Zhou","doi":"10.56028/aetr.8.1.259.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.259.2023","url":null,"abstract":"In the process of preparing α-hemihydrous gypsum from desulfurized gypsum under atmospheric pressure, the optimum conditions of crystallization transformation and modification of desulfurized gypsum were studied. The effects of the type and concentration of composite salts (CaCl2, KCl, MgCl2 mixed solution) on the crystallization and modification of desulfurized gypsum were studied. The results were further verified by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC/TGA), so as to determine the ideal directional crystallization control index. The experimental results show that temperature, type and concentration of salt solution are the most sensitive factors in the crystallization process of desulfurized gypsum. The optimal control conditions for obtaining α-hemihydrous gypsum with good crystalline form are temperature 95℃~98℃, concentration of composite salt solution (CaCl2: MgCl2: KCl = 25:5:1) is 25%~30%, and slurry concentration is greater than 20%.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-07DOI: 10.56028/aetr.8.1.209.2023
Siyuan Liu, Tianshan Liu
In this experiment, bacteria were isolated by aseptically collecting tissue samples of heart, spleen, liver, lymph node, and joint fluid from diseased and dead pigs, and were identified by colony morphology observation, Gram staining microscopy, biochemical experiments, PCR identification, pathogenicity test, and drug sensitivity test. The results showed that the isolated strains were Gram-positive, long-chained, or bipartite coccoid bacteria. It could ferment most sugars, and the VP test and indigo substrate test were negative, consistent with the physiological characteristics of Streptococcus suis. The PCR method was used to identify the serotypes, and about 500 bp of podocarp serotype 9-specific bands could be amplified. Pathogenicity test showed that mice became depressed 6h after intraperitoneal inoculation with the isolate, and died within 24h after rapid breathing, slow movement, and loss of appetite. The results of the drug sensitivity test showed that the isolate was highly sensitive to cefthiophene, florfenicol, cefadroxil, ciprofloxacin, ampicillin, amoxicillin, highly sensitive to sulfisoxazole, polymyxin B, enrofloxacin, doxycycline, and showed resistance to lincomycin, kanamycin, neomycin, gentamicin.
{"title":"Isolation and characterization of Streptococcus suis from pig farms in Sichuan","authors":"Siyuan Liu, Tianshan Liu","doi":"10.56028/aetr.8.1.209.2023","DOIUrl":"https://doi.org/10.56028/aetr.8.1.209.2023","url":null,"abstract":"In this experiment, bacteria were isolated by aseptically collecting tissue samples of heart, spleen, liver, lymph node, and joint fluid from diseased and dead pigs, and were identified by colony morphology observation, Gram staining microscopy, biochemical experiments, PCR identification, pathogenicity test, and drug sensitivity test. The results showed that the isolated strains were Gram-positive, long-chained, or bipartite coccoid bacteria. It could ferment most sugars, and the VP test and indigo substrate test were negative, consistent with the physiological characteristics of Streptococcus suis. The PCR method was used to identify the serotypes, and about 500 bp of podocarp serotype 9-specific bands could be amplified. Pathogenicity test showed that mice became depressed 6h after intraperitoneal inoculation with the isolate, and died within 24h after rapid breathing, slow movement, and loss of appetite. The results of the drug sensitivity test showed that the isolate was highly sensitive to cefthiophene, florfenicol, cefadroxil, ciprofloxacin, ampicillin, amoxicillin, highly sensitive to sulfisoxazole, polymyxin B, enrofloxacin, doxycycline, and showed resistance to lincomycin, kanamycin, neomycin, gentamicin.","PeriodicalId":502380,"journal":{"name":"Advances in Engineering Technology Research","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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