Pub Date : 2023-12-24DOI: 10.31612/2616-4868.7.2023.09
Yaroslava V. Korost, Anastasiia K. Shkvarok
The aim: To study the problem of insomnia in Ukrainians during the first 11 months of the martial law in the country. To determine the predominant type of insomnia in the population. To calculate and assess the risk indicator for the development of cardiovascular complaints in the context of deteriorating sleep quality during the martial law and the risk ratio. Materials and methods: Anamnestic, statistical and calculative methods were used. 321 participants from Ukrainian population took part in an anonymous online survey. 210 respondents were female (65.4 %) and 111 were male (34.6 %). The age distribution was as follows: 18-19 years old (23.1 %), 20-21 years old (14 %), 22-23 years old (28.7 %), 24-25 years old (8.1 %), 26-27 years old (6.2 %), 28-29 years old (6.2 %), 30 years and older (13.7 %). The questionnaire included several sections: general questions regarding the respondents’ characteristics and presence of sleep disorders; questions of the Pittsburgh Sleep Quality Index (PSQI) [2]; subjective assessment of the sleep quality of the respondents during the first 11 months of the martial law and the presence of cardiovascular complaints that appeared or intensified during this period; details of mentioned complaints. Results: 245 respondents (76.3 % of 321) of the entire sample reported about sleep disturbances that developed after February 24, 2022. According to the PSQІ, only 17 respondents (7 %) scored 0-7 points, indicating the absence of insomnia, 180 respondents (73 %) scored 8-14 points, suggesting subclinical insomnia, 48 respondents (20 %) scored 15-21 points, indicating clinically significant insomnia. 209 (85.3 %) attributed these changes to the wartime events in the country. The most significant deterioration in sleep quality during the first 11 months of martial law in the country was observed in March 2022 and the best level of sleep quality was indicated in July. The odds ratio in risk calculation was 3.01; the significance level of the obtained data is 0.006. Conclusions: Subclinical insomnia dominates among Ukrainians during the martial law in the country. There is an increased risk of cardiovascular complaints in the population of Ukraine in the presence of clinically significant sleep disorders related to the martial law. Prolonged exposure to stress for Ukrainians can result in deteriorating sleep quality and increasing in cardiovascular complaints.
{"title":"ASSESSMENT OF SLEEP QUALITY IN UKRAINIANS DURING THE MARTIAL LAW AND THE RISK OF CARDIOVASCULAR COMPLAINTS DEVELOPMENT IN THE CONTEXT OF CLINICALLY SEVERE INSOMNIA","authors":"Yaroslava V. Korost, Anastasiia K. Shkvarok","doi":"10.31612/2616-4868.7.2023.09","DOIUrl":"https://doi.org/10.31612/2616-4868.7.2023.09","url":null,"abstract":"The aim: To study the problem of insomnia in Ukrainians during the first 11 months of the martial law in the country. To determine the predominant type of insomnia in the population. To calculate and assess the risk indicator for the development of cardiovascular complaints in the context of deteriorating sleep quality during the martial law and the risk ratio. Materials and methods: Anamnestic, statistical and calculative methods were used. 321 participants from Ukrainian population took part in an anonymous online survey. 210 respondents were female (65.4 %) and 111 were male (34.6 %). The age distribution was as follows: 18-19 years old (23.1 %), 20-21 years old (14 %), 22-23 years old (28.7 %), 24-25 years old (8.1 %), 26-27 years old (6.2 %), 28-29 years old (6.2 %), 30 years and older (13.7 %). The questionnaire included several sections: general questions regarding the respondents’ characteristics and presence of sleep disorders; questions of the Pittsburgh Sleep Quality Index (PSQI) [2]; subjective assessment of the sleep quality of the respondents during the first 11 months of the martial law and the presence of cardiovascular complaints that appeared or intensified during this period; details of mentioned complaints. Results: 245 respondents (76.3 % of 321) of the entire sample reported about sleep disturbances that developed after February 24, 2022. According to the PSQІ, only 17 respondents (7 %) scored 0-7 points, indicating the absence of insomnia, 180 respondents (73 %) scored 8-14 points, suggesting subclinical insomnia, 48 respondents (20 %) scored 15-21 points, indicating clinically significant insomnia. 209 (85.3 %) attributed these changes to the wartime events in the country. The most significant deterioration in sleep quality during the first 11 months of martial law in the country was observed in March 2022 and the best level of sleep quality was indicated in July. The odds ratio in risk calculation was 3.01; the significance level of the obtained data is 0.006. Conclusions: Subclinical insomnia dominates among Ukrainians during the martial law in the country. There is an increased risk of cardiovascular complaints in the population of Ukraine in the presence of clinically significant sleep disorders related to the martial law. Prolonged exposure to stress for Ukrainians can result in deteriorating sleep quality and increasing in cardiovascular complaints.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"1980 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139160432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-24DOI: 10.31612/2616-4868.7.2023.13
V. Vozniuk, T. A. Vezhnovets
The aim of the study was to determine the model for predicting the risk of dissatisfaction of general practitioners (GPs) with their work in primary health care centers based on the results of a questionnaire. Materials and methods. A survey of 89 GPs was conducted using a questionnaire to determine job satisfaction. Each question of the questionnaire was evaluated by the respondents on the Likert total ratings on a 5-point scale. Job satisfaction was assessed at 4 and 5 points, dissatisfaction at 1-3 points. The logistic regression analysis was used to build a model for risk prediction of GPs’ job dissatisfaction. To assess the degree of each factor influence in the logistic regression model, the odds ratio (OR) and its 95 % CI (confidence interval) were calculated. Receiver Operating Characteristic analysis was used to assess the quality of the logistic regression model. The analysis of the research results was carried out using the license package the statistical analysis package MedCalc v.19.4.1 (MedCalc Software Inc, Broekstraat, Belgium, 1993-2020). The results show that 76.06 % of GPs are satisfied with their work. The vast majority are satisfied with the attitude of the management staff, fellow GPs, patients, opportunities of professional development and advanced training, work schedule, working conditions, and team spirit. At the same time, more than half of GPs (54.92 %) are dissatisfied with the comfort of business relations with other subspecialists, 36.61 % of doctors are dissatisfied with the salary, 40.84 % believe that the salary does not correspond to the efforts made, and 45.07 % think that additional financial compensation does not affect the quality of medical care provided. The method of stepwise inclusion/exclusion of variables was used to select a set of features for the multivariate logistic regression model. As a result of this method, 2 factors were selected: a sense of pride in work and working conditions. We established that the more a GP is proud of his chosen specialty and the better working conditions he has, the less dissatisfied he feels with his work. We determined the sensitivity of the model as 76.5 % (95 % CI: 50.1 % – 93.2 %), the specificity as 85.2 % (95 % CI: 72.9 % – 93.4 %), and the diagnostic accuracy as 83.1 % (CI: 72.3 % – 91 %). Conclusions. In the personnel management system of health care institutions, it is necessary to consider many different factors of GPs’ satisfaction with their work, including salaries, relationships with colleagues, patients, the opportunities of professional development, the attitude of management staff, a sense of respect, and working conditions. Forecasting the risk of GPs’ dissatisfaction with their work must be carried out taking into account the presence of doctors’ subjective sense of pride in their work and working conditions.
{"title":"MODELS FOR PREDICTING THE RISK OF DISSATISFACTION OF GENERAL PRACTITIONERS-FAMILY DOCTORS WITH WORK IN PRIMARY HEALTH CARE CENTERS","authors":"V. Vozniuk, T. A. Vezhnovets","doi":"10.31612/2616-4868.7.2023.13","DOIUrl":"https://doi.org/10.31612/2616-4868.7.2023.13","url":null,"abstract":"The aim of the study was to determine the model for predicting the risk of dissatisfaction of general practitioners (GPs) with their work in primary health care centers based on the results of a questionnaire. Materials and methods. A survey of 89 GPs was conducted using a questionnaire to determine job satisfaction. Each question of the questionnaire was evaluated by the respondents on the Likert total ratings on a 5-point scale. Job satisfaction was assessed at 4 and 5 points, dissatisfaction at 1-3 points. The logistic regression analysis was used to build a model for risk prediction of GPs’ job dissatisfaction. To assess the degree of each factor influence in the logistic regression model, the odds ratio (OR) and its 95 % CI (confidence interval) were calculated. Receiver Operating Characteristic analysis was used to assess the quality of the logistic regression model. The analysis of the research results was carried out using the license package the statistical analysis package MedCalc v.19.4.1 (MedCalc Software Inc, Broekstraat, Belgium, 1993-2020). The results show that 76.06 % of GPs are satisfied with their work. The vast majority are satisfied with the attitude of the management staff, fellow GPs, patients, opportunities of professional development and advanced training, work schedule, working conditions, and team spirit. At the same time, more than half of GPs (54.92 %) are dissatisfied with the comfort of business relations with other subspecialists, 36.61 % of doctors are dissatisfied with the salary, 40.84 % believe that the salary does not correspond to the efforts made, and 45.07 % think that additional financial compensation does not affect the quality of medical care provided. The method of stepwise inclusion/exclusion of variables was used to select a set of features for the multivariate logistic regression model. As a result of this method, 2 factors were selected: a sense of pride in work and working conditions. We established that the more a GP is proud of his chosen specialty and the better working conditions he has, the less dissatisfied he feels with his work. We determined the sensitivity of the model as 76.5 % (95 % CI: 50.1 % – 93.2 %), the specificity as 85.2 % (95 % CI: 72.9 % – 93.4 %), and the diagnostic accuracy as 83.1 % (CI: 72.3 % – 91 %). Conclusions. In the personnel management system of health care institutions, it is necessary to consider many different factors of GPs’ satisfaction with their work, including salaries, relationships with colleagues, patients, the opportunities of professional development, the attitude of management staff, a sense of respect, and working conditions. Forecasting the risk of GPs’ dissatisfaction with their work must be carried out taking into account the presence of doctors’ subjective sense of pride in their work and working conditions.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"423 2‐3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139160700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-24DOI: 10.31612/2616-4868.7.2023.12
Viktoriia V. Horachuk, Nataliia Yu. Kondratiuk, Yurii B. Yashchenko, Mykhailo D. Dyachuk
The aim: to investigate changes in the interpretation of the content of the characteristics of the quality of medical care and medical service; to propose practical approaches to the formation of modern quality criteria and indicators in health care institutions. Materials and methods: sources of scientific literature from the databases PubMed, NICE, according to the selected research topic, were selected as research materials; methods: bibliosemantic; analysis and generalization of the obtained data. Results. Classic characteristics of quality – safety, quality of resources, quality of the medical care process, treatment outcome, patient satisfaction, timeliness, fairness, efficiency – are reviewed over time and acquire additional or radically changed content. The selection of relevant quality characteristics is important for health care managers to define quality criteria and indicators according to the requirements of the local context. In addition to the traditional components of infectious safety and the safety of medical interventions, the safety criterion is today complemented by the components of radiation, environmental, epidemic, physical, informational, and terrorist safety. Safety also refers to the ability of professionals to avoid, prevent and reduce harmful interventions or risks to themselves and the environment. Patient-centeredness must include respect for the relationship not only between physician and patient, but also between the patient and all providers of health care for that patient. Effective, safe health care is reflected in a culture of excellence that includes collaboration, communication, compassion, competence, advocacy, respect, accountability and reliability. The most relevant criteria and indicators of the quality of medical care must be developed in accordance with the structure of innovative organizational models of academic health care centers, where scientific, clinical and educational activities are integrated. This requires the formation of end-to-end quality criteria and indicators that cover all activities of academic centers. Conclusions: criteria and indicators of the quality of medical care and medical service are constantly supplemented with new components, in accordance with the achievements of medical science and practice. The transformation of the organizational structures of medical care into academic centers, where scientific and educational activities are added to traditional medical practice, requires the development and introduction of end-to-end criteria for the quality of medical care.
{"title":"DEVELOPMENT AND IMPROVEMENT OF QUALITY CHARACTERISTICS OF MEDICAL CARE","authors":"Viktoriia V. Horachuk, Nataliia Yu. Kondratiuk, Yurii B. Yashchenko, Mykhailo D. Dyachuk","doi":"10.31612/2616-4868.7.2023.12","DOIUrl":"https://doi.org/10.31612/2616-4868.7.2023.12","url":null,"abstract":"The aim: to investigate changes in the interpretation of the content of the characteristics of the quality of medical care and medical service; to propose practical approaches to the formation of modern quality criteria and indicators in health care institutions. Materials and methods: sources of scientific literature from the databases PubMed, NICE, according to the selected research topic, were selected as research materials; methods: bibliosemantic; analysis and generalization of the obtained data. Results. Classic characteristics of quality – safety, quality of resources, quality of the medical care process, treatment outcome, patient satisfaction, timeliness, fairness, efficiency – are reviewed over time and acquire additional or radically changed content. The selection of relevant quality characteristics is important for health care managers to define quality criteria and indicators according to the requirements of the local context. In addition to the traditional components of infectious safety and the safety of medical interventions, the safety criterion is today complemented by the components of radiation, environmental, epidemic, physical, informational, and terrorist safety. Safety also refers to the ability of professionals to avoid, prevent and reduce harmful interventions or risks to themselves and the environment. Patient-centeredness must include respect for the relationship not only between physician and patient, but also between the patient and all providers of health care for that patient. Effective, safe health care is reflected in a culture of excellence that includes collaboration, communication, compassion, competence, advocacy, respect, accountability and reliability. The most relevant criteria and indicators of the quality of medical care must be developed in accordance with the structure of innovative organizational models of academic health care centers, where scientific, clinical and educational activities are integrated. This requires the formation of end-to-end quality criteria and indicators that cover all activities of academic centers. Conclusions: criteria and indicators of the quality of medical care and medical service are constantly supplemented with new components, in accordance with the achievements of medical science and practice. The transformation of the organizational structures of medical care into academic centers, where scientific and educational activities are added to traditional medical practice, requires the development and introduction of end-to-end criteria for the quality of medical care.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"2018 45","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-24DOI: 10.31612/2616-4868.7.2023.08
K. Duve
Introduction: Predicting the risk of cognitive dysfunction and disability in everyday life among chronic alcohol-induced encephalopathy (CAIE) patients allows timely treatment to prevent dementia. The aim: to develop a mathematical model for predicting the risk of developing cognitive disorders and functional disability in patients with CAIE based on the analysis of polymorphic variants of the genes ACE, AT2R1, eNOS, PON1, IL-1β, IL-10, TNF-α, as well as cofactors (gender, age group, the disease duration, and presence/absence of somatic comorbidity). Materials and methods: We examined the 102 patients with CAIE who were undergoing inpatient treatment in the communal non-profit enterprise «Ternopil Regional Clinical Psychoneurological Hospital» during 2021-2022 and were included in the retrospective analysis. The molecular-genetic testing was performed for 26 patients of the general group. The molecular-genetic differentiation of the studied polymorphic variants of genes was carried out in the molecular-genetic laboratory of the state institution «Reference Centre for Molecular Diagnostics of the Ministry of Health of Ukraine», Kyiv. Cognitive functions were assessed using the Montreal Cognitive Test (MoCA); activities of daily living were assessed using the Barthel index. A logistic regression analysis was performed to determine the risk of cognitive impairment and functional disability developing in patients with CAIE. Results: Analyzing polymorphic variants of the ACE, AT2R1, eNOS, PON1, IL-1β, IL-10, TNF-α genes, as well as such cofactors as gender, age group, history and presence/absence of somatic comorbidity in the context of the development of cognitive disorders in patients with CAIE it has been established that the C108T polymorphism of the PON1 gene has the most significant prognostic value (in the presence of the T/T genotype, the probability of cognitive impairment is 39.84 %). At the same time, carriage of both the T allele and the C allele of the PON1 gene is associated with a significant decrease in the MoCA score. Regarding the development of functional disability in patients with CAIE, the C108T polymorphism of the PON1 gene also has the most significant prognostic value (in the presence of the T/T genotype, the probability of functional disability is 48.08 %, respectively, C/C – 30.96 % and CT – 39.22 %, however, no statistically significant differences in the Barthel index values were found in carriers of the above genotypes). Conclusions: It was established that the C108T polymorphism of the PON1 gene is significantly associated with the development of cognitive impairment and functional disability in patients with CAIE, which indicates the feasibility of further studies involving a larger sample of patients.
{"title":"CHRONIC ALCOHOL-INDUCED ENCEPHALOPATHY: PREDICTORS OF COGNITIVE IMPAIRMENT AND FUNCTIONAL DISABILITY","authors":"K. Duve","doi":"10.31612/2616-4868.7.2023.08","DOIUrl":"https://doi.org/10.31612/2616-4868.7.2023.08","url":null,"abstract":"Introduction: Predicting the risk of cognitive dysfunction and disability in everyday life among chronic alcohol-induced encephalopathy (CAIE) patients allows timely treatment to prevent dementia. The aim: to develop a mathematical model for predicting the risk of developing cognitive disorders and functional disability in patients with CAIE based on the analysis of polymorphic variants of the genes ACE, AT2R1, eNOS, PON1, IL-1β, IL-10, TNF-α, as well as cofactors (gender, age group, the disease duration, and presence/absence of somatic comorbidity). Materials and methods: We examined the 102 patients with CAIE who were undergoing inpatient treatment in the communal non-profit enterprise «Ternopil Regional Clinical Psychoneurological Hospital» during 2021-2022 and were included in the retrospective analysis. The molecular-genetic testing was performed for 26 patients of the general group. The molecular-genetic differentiation of the studied polymorphic variants of genes was carried out in the molecular-genetic laboratory of the state institution «Reference Centre for Molecular Diagnostics of the Ministry of Health of Ukraine», Kyiv. Cognitive functions were assessed using the Montreal Cognitive Test (MoCA); activities of daily living were assessed using the Barthel index. A logistic regression analysis was performed to determine the risk of cognitive impairment and functional disability developing in patients with CAIE. Results: Analyzing polymorphic variants of the ACE, AT2R1, eNOS, PON1, IL-1β, IL-10, TNF-α genes, as well as such cofactors as gender, age group, history and presence/absence of somatic comorbidity in the context of the development of cognitive disorders in patients with CAIE it has been established that the C108T polymorphism of the PON1 gene has the most significant prognostic value (in the presence of the T/T genotype, the probability of cognitive impairment is 39.84 %). At the same time, carriage of both the T allele and the C allele of the PON1 gene is associated with a significant decrease in the MoCA score. Regarding the development of functional disability in patients with CAIE, the C108T polymorphism of the PON1 gene also has the most significant prognostic value (in the presence of the T/T genotype, the probability of functional disability is 48.08 %, respectively, C/C – 30.96 % and CT – 39.22 %, however, no statistically significant differences in the Barthel index values were found in carriers of the above genotypes). Conclusions: It was established that the C108T polymorphism of the PON1 gene is significantly associated with the development of cognitive impairment and functional disability in patients with CAIE, which indicates the feasibility of further studies involving a larger sample of patients.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"1985 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139160605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-24DOI: 10.31612/2616-4868.7.2023.02
T. Osadchuk, Andrii V. Kalashnikov, Oleg A. Kostohryz, Volodymyr V. Protsenko
Introduction. Osteoarthritis affects 7 % of the world’s population. The number of affected people worldwide increased by 48 % from 1990 to 2019. At the initial stages of knee osteoarthritis treatment, conservative treatment and corrective osteotomies are used. Corrective osteotomies in the area of the knee joint have shown excellent results for the treatment of limited arthrosis with a predominant lesion of the medial part of the joint in relatively young patients. However, despite the good results reported in the literature, patient satisfaction usually deteriorates over time, and some inevitably require further treatment with total knee arthroplasty in cases of progression of osteoarthritis. The aim of the study. On the basis of a retrospective analysis of surgical treatment of patients with osteoarthritis of the knee joints, justify the differentiated use of surgical interventions. Materials and methods. The article considers a differentiated approach to peri-articular osteotomies and primary endoprosthesis in gonarthrosis, in particular, in the focus of the endoprosthesis selection algorithm, based on related factors, such as the stage of the disease, the patient’s age, and the presence of concomitant complications, including the presence or absence of aseptic necrosis, contractures, instability ligamentous apparatus and defects of the condyles of the tibial bone. 391 knee joints were operated on in 383 patients. Results. In general, good results were obtained in 65 % (210 people) of endoprosthetics. The patients felt well, did not feel pain, did not limp, the range of motion was restored within normal limits, there was no deformation of the joint, the axis of the limb was correct, the joint was stable. Satisfactory results were obtained in 35 % (114 people), the patients felt well, did not feel pain, moderate lameness remained, range of motion was limited to no more than 800, there was no joint deformation, the axis of the limb was correct, the joint was stable. No unsatisfactory results were obtained. Conclusions. The developed differentiated approach to operative treatment of patients with osteoarthritis of the knee joints allowed to obtain 65 % good and 35 % satisfactory results. The prospect of further development in this direction continues due to the improvement of the design of endoprostheses and the technique of surgical intervention
{"title":"SURGICAL TREATMENT OF PATIENTS WITH OSTEOARTHRITIS OF THE KNEE JOINTS","authors":"T. Osadchuk, Andrii V. Kalashnikov, Oleg A. Kostohryz, Volodymyr V. Protsenko","doi":"10.31612/2616-4868.7.2023.02","DOIUrl":"https://doi.org/10.31612/2616-4868.7.2023.02","url":null,"abstract":"Introduction. Osteoarthritis affects 7 % of the world’s population. The number of affected people worldwide increased by 48 % from 1990 to 2019. At the initial stages of knee osteoarthritis treatment, conservative treatment and corrective osteotomies are used. Corrective osteotomies in the area of the knee joint have shown excellent results for the treatment of limited arthrosis with a predominant lesion of the medial part of the joint in relatively young patients. However, despite the good results reported in the literature, patient satisfaction usually deteriorates over time, and some inevitably require further treatment with total knee arthroplasty in cases of progression of osteoarthritis. The aim of the study. On the basis of a retrospective analysis of surgical treatment of patients with osteoarthritis of the knee joints, justify the differentiated use of surgical interventions. Materials and methods. The article considers a differentiated approach to peri-articular osteotomies and primary endoprosthesis in gonarthrosis, in particular, in the focus of the endoprosthesis selection algorithm, based on related factors, such as the stage of the disease, the patient’s age, and the presence of concomitant complications, including the presence or absence of aseptic necrosis, contractures, instability ligamentous apparatus and defects of the condyles of the tibial bone. 391 knee joints were operated on in 383 patients. Results. In general, good results were obtained in 65 % (210 people) of endoprosthetics. The patients felt well, did not feel pain, did not limp, the range of motion was restored within normal limits, there was no deformation of the joint, the axis of the limb was correct, the joint was stable. Satisfactory results were obtained in 35 % (114 people), the patients felt well, did not feel pain, moderate lameness remained, range of motion was limited to no more than 800, there was no joint deformation, the axis of the limb was correct, the joint was stable. No unsatisfactory results were obtained. Conclusions. The developed differentiated approach to operative treatment of patients with osteoarthritis of the knee joints allowed to obtain 65 % good and 35 % satisfactory results. The prospect of further development in this direction continues due to the improvement of the design of endoprostheses and the technique of surgical intervention","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"308 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139160716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.31612/2616-4868.6.2023.07
M. P. Cherkun
Introduction: at present, hypertension is the most common non-infectious disease worldwide, with a progressively growing number of patients each year. This escalating prevalence results in increased disability and early mortality in both rural and urban populations. Currently, it is believed that psychosomatic disorders contribute to the progression of hypertension. Consequently, a comprehensive examination of this relationship is highly relevant. The aim of the research is to analyze the presence of anxiety and depression in patients with isolated and comorbid hypertensive disease and to determine their influence on mental well-being, depending on the territorial affiliation of the patients. Materials and methods: we analyzed the results of treatment of 152 patients with isolated and comorbid (ischemic heart disease, type 2 diabetes) course of hypertensive disease. These patients received medical care at the Municipal Enterprise “Poltava Regional Clinical Medical Cardiovascular Center of the Poltava Regional Council” between 2018 and 2022. We categorized these patients into three groups based on the course of hypertension. Group 1 consisted of 45 patients with an isolated presentation (26 (57.8%) residing in urban areas and 19 (42.2%) in rural settings). Group 2 comprised 68 patients with a combined presentation of hypertension and coronary heart disease, with an even distribution between urban (34 (50.0%)) and rural (34 (50.0%)) backgrounds. Group 3 encompassed 39 patients with a combined presentation of hypertension, coronary heart disease, and type 2 diabetes, with 24 patients (61.5%) from urban areas and 15 (48.5%) from rural areas. Patients in all groups were comparable in terms of age (ranging from 22 to 85 years), gender, and disease duration. Anxiety and depression levels were assessed using the HADS scale, and quality of life was evaluated using the SF-36 scale through self-questionnaires. Results: the study revealed a notable decline in the mental component of quality of life as comorbidity increased, affecting both rural and urban residents. At the same time, a corresponding increase in the levels of anxiety and depression was observed with escalating comorbidity. A strong inverse correlation was established between the levels of anxiety and depression and all indicators of the mental component of quality of life (r from -0.94 to -0.91). Furthermore, the research highlighted that in patients with an isolated presentation of hypertension, psychosomatic factors exerted the most substantial influence on the mental health index (MH). In contrast, for patients with comorbid conditions, the presence of psychosomatic factors more frequently impacted the levels of social functioning (SF) and vitality (VT). Conclusions: Patients residing in rural areas who have comorbid conditions such as hypertensive disease, coronary heart disease, and type 2 diabetes are particularly susceptible to experiencing anxiety and depression.
{"title":"THE IMPACT OF ANXIETY AND DEPRESSION ON THE MENTAL WELL-BEING OF PATIENTS WITH HYPERTENSIVE DISEASE","authors":"M. P. Cherkun","doi":"10.31612/2616-4868.6.2023.07","DOIUrl":"https://doi.org/10.31612/2616-4868.6.2023.07","url":null,"abstract":"Introduction: at present, hypertension is the most common non-infectious disease worldwide, with a progressively growing number of patients each year. This escalating prevalence results in increased disability and early mortality in both rural and urban populations. Currently, it is believed that psychosomatic disorders contribute to the progression of hypertension. Consequently, a comprehensive examination of this relationship is highly relevant. The aim of the research is to analyze the presence of anxiety and depression in patients with isolated and comorbid hypertensive disease and to determine their influence on mental well-being, depending on the territorial affiliation of the patients. Materials and methods: we analyzed the results of treatment of 152 patients with isolated and comorbid (ischemic heart disease, type 2 diabetes) course of hypertensive disease. These patients received medical care at the Municipal Enterprise “Poltava Regional Clinical Medical Cardiovascular Center of the Poltava Regional Council” between 2018 and 2022. We categorized these patients into three groups based on the course of hypertension. Group 1 consisted of 45 patients with an isolated presentation (26 (57.8%) residing in urban areas and 19 (42.2%) in rural settings). Group 2 comprised 68 patients with a combined presentation of hypertension and coronary heart disease, with an even distribution between urban (34 (50.0%)) and rural (34 (50.0%)) backgrounds. Group 3 encompassed 39 patients with a combined presentation of hypertension, coronary heart disease, and type 2 diabetes, with 24 patients (61.5%) from urban areas and 15 (48.5%) from rural areas. Patients in all groups were comparable in terms of age (ranging from 22 to 85 years), gender, and disease duration. Anxiety and depression levels were assessed using the HADS scale, and quality of life was evaluated using the SF-36 scale through self-questionnaires. Results: the study revealed a notable decline in the mental component of quality of life as comorbidity increased, affecting both rural and urban residents. At the same time, a corresponding increase in the levels of anxiety and depression was observed with escalating comorbidity. A strong inverse correlation was established between the levels of anxiety and depression and all indicators of the mental component of quality of life (r from -0.94 to -0.91). Furthermore, the research highlighted that in patients with an isolated presentation of hypertension, psychosomatic factors exerted the most substantial influence on the mental health index (MH). In contrast, for patients with comorbid conditions, the presence of psychosomatic factors more frequently impacted the levels of social functioning (SF) and vitality (VT). Conclusions: Patients residing in rural areas who have comorbid conditions such as hypertensive disease, coronary heart disease, and type 2 diabetes are particularly susceptible to experiencing anxiety and depression.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"26 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.31612/2616-4868.6.2023.05
L. Strashok, S. Turchina, G. Kosovtsova, M. Isakova, Alla V. Yeshchenko, M. Khomenko
Introduction. The incidence of diabetes mellitus in the world among children is steadily increasing. The main striking factor in type 1 diabetes mellitus (T1DM) is hyperglycemia, which affects the formation of liver complications such as glycogenic hepatopathy and non-alcoholic fatty liver disease (NAFLD). The aim of the study is to determine the prognostic signs of hepatopathy formation in adolescents with type 1 diabetes mellitus. Materials and methods. Clinical-anamnestic and laboratory-instrumental examination: glycosylated hemoglobin (HbA1c), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin and its fractions, lipidogram. According to the results of ultrasound of the hepatobiliary system, girls and boys with T1DM were divided into groups taking into account the size of the liver. The study included 87 girls and 86 boys aged 8-18 years. Methods of mathematical statistics, system-information analysis, Wald’s heterogeneous sequential statistical procedure with Kulbak’s information measure were used. Results. A comprehensive examination of adolescents with T1DM revealed that 70% of patients had digestive system disorders in the form of pain and dyspeptic syndromes. In 88% of boys and 68% of girls, palpation and ultrasound revealed liver enlargement (χ2 < 0,05). Atherogenic orientation of lipidogram was determined in all adolescents with T1DM. In girls, the difference in β-lipoprotein level and atherogenicity coefficient reached statistical significance compared with boys. In most adolescents, the content of high-density lipoprotein cholesterol was within normal limits, but the frequency of its decrease in boys with liver enlargement was greater compared with girls with liver enlargement (32% and 24%, respectively, χ2 < 0,05). ALT activity significantly exceeded the upper limit of normal only in the group of girls with liver enlargement. Increased ALT activity in girls with enlarged liver appeared 1.5 times more often than in boys of the similar group (respectively 68% and 43%, χ2 < 0,05). Conclusions. In boys with T1DM, significant informative prognostic factors for the formation of hepatopathy are the duration of the disease, increased levels of HbA1c, atherogenic lipoprotein fractions, and ALT activity. In girls with T1DM, the most significant prognostic factor was an increase in ALT activity; the overall informativeness of lipid profile parameters in girls was three times lower compared with boys. The duration of the disease and the degree of compensation were less significant for the formation of hepatopathy in girls.
{"title":"PROGNOSTIC SIGNS OF HEPATOPATHY FORMATION IN ADOLESCENTS WITH TYPE 1 DIABETES MELLITUS","authors":"L. Strashok, S. Turchina, G. Kosovtsova, M. Isakova, Alla V. Yeshchenko, M. Khomenko","doi":"10.31612/2616-4868.6.2023.05","DOIUrl":"https://doi.org/10.31612/2616-4868.6.2023.05","url":null,"abstract":"Introduction. The incidence of diabetes mellitus in the world among children is steadily increasing. The main striking factor in type 1 diabetes mellitus (T1DM) is hyperglycemia, which affects the formation of liver complications such as glycogenic hepatopathy and non-alcoholic fatty liver disease (NAFLD). The aim of the study is to determine the prognostic signs of hepatopathy formation in adolescents with type 1 diabetes mellitus. Materials and methods. Clinical-anamnestic and laboratory-instrumental examination: glycosylated hemoglobin (HbA1c), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin and its fractions, lipidogram. According to the results of ultrasound of the hepatobiliary system, girls and boys with T1DM were divided into groups taking into account the size of the liver. The study included 87 girls and 86 boys aged 8-18 years. Methods of mathematical statistics, system-information analysis, Wald’s heterogeneous sequential statistical procedure with Kulbak’s information measure were used. Results. A comprehensive examination of adolescents with T1DM revealed that 70% of patients had digestive system disorders in the form of pain and dyspeptic syndromes. In 88% of boys and 68% of girls, palpation and ultrasound revealed liver enlargement (χ2 < 0,05). Atherogenic orientation of lipidogram was determined in all adolescents with T1DM. In girls, the difference in β-lipoprotein level and atherogenicity coefficient reached statistical significance compared with boys. In most adolescents, the content of high-density lipoprotein cholesterol was within normal limits, but the frequency of its decrease in boys with liver enlargement was greater compared with girls with liver enlargement (32% and 24%, respectively, χ2 < 0,05). ALT activity significantly exceeded the upper limit of normal only in the group of girls with liver enlargement. Increased ALT activity in girls with enlarged liver appeared 1.5 times more often than in boys of the similar group (respectively 68% and 43%, χ2 < 0,05). Conclusions. In boys with T1DM, significant informative prognostic factors for the formation of hepatopathy are the duration of the disease, increased levels of HbA1c, atherogenic lipoprotein fractions, and ALT activity. In girls with T1DM, the most significant prognostic factor was an increase in ALT activity; the overall informativeness of lipid profile parameters in girls was three times lower compared with boys. The duration of the disease and the degree of compensation were less significant for the formation of hepatopathy in girls.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"389 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.31612/2616-4868.6.2023.06
Yulia Z. Kotsyubynska, V. Voloshynovych, Yuriy I. Solodjuk, Valentyna I. Liampel, Vasyl L. Fentsyk
Introduction. Artificial neural networks are widely utilized in medical fields, such as dentistry, molecular genetics, immunology, cardiology, and others. Forensic medicine is no exception, as artificial neural networks are also beginning to find applications in this field. The aim of this study was to demonstrate the potential for predicting human anthropometric parameters using dermatoglyphic parameters, which could enhance the method of dermatoglyphic identification. Materials and methods. We analyzed dermatoglyphs of the hands and feet from 567 individuals aged 18 to 59 years, with no genetic or endocrine disorders and no musculoskeletal problems. Results and discussion. The outcome of our work resulted in the development of the "Dermatoglyphics For Prediction (DFP)" program [Author's Certificate No. 74561. Computer program "Forensic Medical Identification Program using Artificial Neural Networks" Registration date: 07.11.2017]. This software device, after appropriate training, enables the prediction of an individual's ethnic-territorial affiliation and the presence of specific anthropometric parameters using such input data as dermatoglyphs of the hands and feet. Conclusions. The increasing needs of the Ukrainian community for the identification of unknown individuals, given the geopolitical situation related to Russian invasion in Ukraine (the constant threat of ballistic missile attacks and unmanned aerial vehicles across the entire territory of Ukraine, which could lead to mass casualties), justify the relevance and the search for innovative approaches to dermatoglyphic identification expertise, utilizing state-of-the-art technologies, particularly neural network-based prediction of anthropometric parameters, sex, and ethnic-territorial affiliation of an unknown person, using input parameters such as dermatoglyphs of the hands and feet, with the aim of enhancing the evidentiary value of identification expertise. This software device, after appropriate training, allows for the prediction of ethnic-territorial affiliation and the presence of specific anthropometric parameters in the examined individual using such input data as dermatoglyphs of the hands and feet.
{"title":"ARTIFICIAL INTELLIGENCE – APPLICATION IN FORENSIC MEDICINE","authors":"Yulia Z. Kotsyubynska, V. Voloshynovych, Yuriy I. Solodjuk, Valentyna I. Liampel, Vasyl L. Fentsyk","doi":"10.31612/2616-4868.6.2023.06","DOIUrl":"https://doi.org/10.31612/2616-4868.6.2023.06","url":null,"abstract":"Introduction. Artificial neural networks are widely utilized in medical fields, such as dentistry, molecular genetics, immunology, cardiology, and others. Forensic medicine is no exception, as artificial neural networks are also beginning to find applications in this field. The aim of this study was to demonstrate the potential for predicting human anthropometric parameters using dermatoglyphic parameters, which could enhance the method of dermatoglyphic identification. Materials and methods. We analyzed dermatoglyphs of the hands and feet from 567 individuals aged 18 to 59 years, with no genetic or endocrine disorders and no musculoskeletal problems. Results and discussion. The outcome of our work resulted in the development of the \"Dermatoglyphics For Prediction (DFP)\" program [Author's Certificate No. 74561. Computer program \"Forensic Medical Identification Program using Artificial Neural Networks\" Registration date: 07.11.2017]. This software device, after appropriate training, enables the prediction of an individual's ethnic-territorial affiliation and the presence of specific anthropometric parameters using such input data as dermatoglyphs of the hands and feet. Conclusions. The increasing needs of the Ukrainian community for the identification of unknown individuals, given the geopolitical situation related to Russian invasion in Ukraine (the constant threat of ballistic missile attacks and unmanned aerial vehicles across the entire territory of Ukraine, which could lead to mass casualties), justify the relevance and the search for innovative approaches to dermatoglyphic identification expertise, utilizing state-of-the-art technologies, particularly neural network-based prediction of anthropometric parameters, sex, and ethnic-territorial affiliation of an unknown person, using input parameters such as dermatoglyphs of the hands and feet, with the aim of enhancing the evidentiary value of identification expertise. This software device, after appropriate training, allows for the prediction of ethnic-territorial affiliation and the presence of specific anthropometric parameters in the examined individual using such input data as dermatoglyphs of the hands and feet.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"89 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.31612/2616-4868.6.2023.11
Nataliia M. Pryplavko, Anatolii M. Kravchenko
Aim: to investigate the relationship between cardiovascular risk (CVR) and different types of diurnal blood pressure (BP) profile among men of working age with arterial hypertension (AH). Materials and methods. The study included 83 men of working age (average age 48±2 years) with II stage, 2 and 3 degrees of AH. The risk of death due to cardiovascular diseases during the next 10 years was assessed using the SCORE scale. All patients underwent ambulatory BP monitoring (ABPM), according to the results of which the following diurnal BP profiles were identified: 23 (28%) patients – normal (optimal) level of nocturnal decrease in BP («dipper»; daily index (DI) 10-20%); 10 (12%) – excessive nocturnal lowering of BP («over-dipper»; DI >20%); 38 (46%) – insufficient nocturnal lowering of BP («non-dipper»; DI <10%); and 12 (14%) patients had an inverted diurnal curve («night-peaker»; DI <0%). Results and discussion. According to the SCORE scale, the CVR profile in «dipper» group (n=23) was as follows: low – 6 (26%) patients; moderate – 15 (65%); and high – 2 (9%). Among 10 patients of «over-dipper» group, 7 had low, 2 – moderate, and 1 – high CVR. In «non-dipper» group (n=38), there were 4 (11%) patients with low CVR, 10 (26%) with moderate, 14 (37%) with high, and 10 (26%) with very high CVR. At the same time, 7 out of 12 patients of «night-peaker» group showed very high CVR, 3 – high, and 2 – moderate (overall p<0,001). The frequency of patients with high CVR was higher in the pooled «non-dipper» and «night-peaker» group (pathological BP profile; n=50), as compared to the pooled «dipper» and «over-dipper» group (n=33): 34% vs. 9%, respectively. At the same time, the pooled «dipper» and «over-dipper» group was characterized by the higher frequency of patients with moderate (52%) and low CVR (39%), in contrast to the pooled «non-dipper» and «night-peaker» group (24% and 8%, respectively). Finally, all 17 patients with very high CVR had an abnormal diurnal BP profile (34% in the pooled «non-dipper» and «night-peaker» group) (overall p<0,001). Conclusions. It was established that, according to ABPM, 60% of men of working age with the AH of II stage, 2- and 3-degrees, had pathological BP profiles of the «non-dipper» and «night-peaker» types. Individuals diagnosed with nocturnal rise or insufficient diurnal BP decrease have a higher risk of cardiovascular death in the next 10 years according to SCORE. Patients with a «non-dipper» and «night-peaker» diurnal BP profiles require more aggressive drug therapy and re-examination with ABPM to control the normalization of the diurnal BP profile.
{"title":"CARDIOVASCULAR RISK AMONG MEN OF WORKING AGE WITH ARTERIAL HYPERTENSION DEPENDING ON THE ARTERIAL PRESSURE DIURNAL PROFILE","authors":"Nataliia M. Pryplavko, Anatolii M. Kravchenko","doi":"10.31612/2616-4868.6.2023.11","DOIUrl":"https://doi.org/10.31612/2616-4868.6.2023.11","url":null,"abstract":"Aim: to investigate the relationship between cardiovascular risk (CVR) and different types of diurnal blood pressure (BP) profile among men of working age with arterial hypertension (AH). Materials and methods. The study included 83 men of working age (average age 48±2 years) with II stage, 2 and 3 degrees of AH. The risk of death due to cardiovascular diseases during the next 10 years was assessed using the SCORE scale. All patients underwent ambulatory BP monitoring (ABPM), according to the results of which the following diurnal BP profiles were identified: 23 (28%) patients – normal (optimal) level of nocturnal decrease in BP («dipper»; daily index (DI) 10-20%); 10 (12%) – excessive nocturnal lowering of BP («over-dipper»; DI >20%); 38 (46%) – insufficient nocturnal lowering of BP («non-dipper»; DI <10%); and 12 (14%) patients had an inverted diurnal curve («night-peaker»; DI <0%). Results and discussion. According to the SCORE scale, the CVR profile in «dipper» group (n=23) was as follows: low – 6 (26%) patients; moderate – 15 (65%); and high – 2 (9%). Among 10 patients of «over-dipper» group, 7 had low, 2 – moderate, and 1 – high CVR. In «non-dipper» group (n=38), there were 4 (11%) patients with low CVR, 10 (26%) with moderate, 14 (37%) with high, and 10 (26%) with very high CVR. At the same time, 7 out of 12 patients of «night-peaker» group showed very high CVR, 3 – high, and 2 – moderate (overall p<0,001). The frequency of patients with high CVR was higher in the pooled «non-dipper» and «night-peaker» group (pathological BP profile; n=50), as compared to the pooled «dipper» and «over-dipper» group (n=33): 34% vs. 9%, respectively. At the same time, the pooled «dipper» and «over-dipper» group was characterized by the higher frequency of patients with moderate (52%) and low CVR (39%), in contrast to the pooled «non-dipper» and «night-peaker» group (24% and 8%, respectively). Finally, all 17 patients with very high CVR had an abnormal diurnal BP profile (34% in the pooled «non-dipper» and «night-peaker» group) (overall p<0,001). Conclusions. It was established that, according to ABPM, 60% of men of working age with the AH of II stage, 2- and 3-degrees, had pathological BP profiles of the «non-dipper» and «night-peaker» types. Individuals diagnosed with nocturnal rise or insufficient diurnal BP decrease have a higher risk of cardiovascular death in the next 10 years according to SCORE. Patients with a «non-dipper» and «night-peaker» diurnal BP profiles require more aggressive drug therapy and re-examination with ABPM to control the normalization of the diurnal BP profile.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"81 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.31612/2616-4868.6.2023.13
V. Voloshynovych, N. Kozan, Viktoriia M. Voloshynovych, Yulia Z. Kotsyubynska
Introduction. Phytoplankton, including cyanobacteria, is ubiquitous in various types of reservoirs, and it plays a crucial role in aquatic ecosystems. Among phytoplankton, cyanobacteria, considered one of the most primitive chlorophyll-containing organisms, have a significant presence in both standing freshwater lakes and seawater. Microcystis aeruginosa (M. aeruginosa), a common cyanobacterial species, is known for forming harmful “water blooms” that pose serious ecological and health risks. The rise of urbanization in Ukraine and the consequences of the war has intensified the eutrophication and cyanobacterial blooms in drinking water sources, necessitating improved detection and prevention strategies. The aim of this study was to develop modern and effective methods for diagnosing microcystin-containing cyanobacteria, particularly M. aeruginosa, to address biological hazards in Ukrainian water reservoirs. Materials and methods. Water samples were collected from a stagnant town lake in the Ivano-Frankivsk region during both summer and winter seasons to account for seasonal fluctuations in cyanobacteria content. Cyanobacteria were isolated from water samples and subjected to DNA extraction. PCR analysis using specific oligonucleotide primers targeted the 16S rRNA gene of M. aeruginosa. The results revealed the presence of 16S rRNA products in all water samples, irrespective of the season, indicating a consistent distribution of M. aeruginosa in the lake throughout the year. Discussion. We found cyanobacteria in all water samples regardless of the season. This indirectly suggests the presence of anthropogenic pollution, which helps maintain a consistent concentration of cyanobacteria throughout the year. In conclusion, the developed PCR-based method offers a sensitive and specific means of detecting M. aeruginosa, enabling comprehensive investigations across various water reservoirs. The presence of cyanobacteria in water bodies, while natural, can serve as an indicator of anthropogenic pollution, particularly nitrogen and phosphorus. This methodology holds promise for safeguarding Ukraine's drinking water sources and managing the impact of anthropogenic pollution, ultimately ensuring public health through effective planning and preventive measures.
{"title":"MICRОCYSTIS AЕRUGINОSA AS A SОURCЕ ОF BIОLОGICAL HAZARDS IN WATЕR RЕSЕRVОIRS ОF UKRAINЕ","authors":"V. Voloshynovych, N. Kozan, Viktoriia M. Voloshynovych, Yulia Z. Kotsyubynska","doi":"10.31612/2616-4868.6.2023.13","DOIUrl":"https://doi.org/10.31612/2616-4868.6.2023.13","url":null,"abstract":"Introduction. Phytoplankton, including cyanobacteria, is ubiquitous in various types of reservoirs, and it plays a crucial role in aquatic ecosystems. Among phytoplankton, cyanobacteria, considered one of the most primitive chlorophyll-containing organisms, have a significant presence in both standing freshwater lakes and seawater. Microcystis aeruginosa (M. aeruginosa), a common cyanobacterial species, is known for forming harmful “water blooms” that pose serious ecological and health risks. The rise of urbanization in Ukraine and the consequences of the war has intensified the eutrophication and cyanobacterial blooms in drinking water sources, necessitating improved detection and prevention strategies. The aim of this study was to develop modern and effective methods for diagnosing microcystin-containing cyanobacteria, particularly M. aeruginosa, to address biological hazards in Ukrainian water reservoirs. Materials and methods. Water samples were collected from a stagnant town lake in the Ivano-Frankivsk region during both summer and winter seasons to account for seasonal fluctuations in cyanobacteria content. Cyanobacteria were isolated from water samples and subjected to DNA extraction. PCR analysis using specific oligonucleotide primers targeted the 16S rRNA gene of M. aeruginosa. The results revealed the presence of 16S rRNA products in all water samples, irrespective of the season, indicating a consistent distribution of M. aeruginosa in the lake throughout the year. Discussion. We found cyanobacteria in all water samples regardless of the season. This indirectly suggests the presence of anthropogenic pollution, which helps maintain a consistent concentration of cyanobacteria throughout the year. In conclusion, the developed PCR-based method offers a sensitive and specific means of detecting M. aeruginosa, enabling comprehensive investigations across various water reservoirs. The presence of cyanobacteria in water bodies, while natural, can serve as an indicator of anthropogenic pollution, particularly nitrogen and phosphorus. This methodology holds promise for safeguarding Ukraine's drinking water sources and managing the impact of anthropogenic pollution, ultimately ensuring public health through effective planning and preventive measures.","PeriodicalId":502404,"journal":{"name":"Clinical and Preventive Medicine","volume":"111 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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