首页 > 最新文献

International Journal of Medical Microbiology最新文献

英文 中文
Interdisciplinary studies on Coxiella burnetii: From molecular to cellular, to host, to one health research 关于烧伤柯西氏菌的跨学科研究:从分子研究到细胞研究,再到宿主研究,最后到健康研究
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.1016/j.ijmm.2023.151590
Benjamin U. Bauer , Michael R. Knittler , Jennifer Andrack , Christian Berens , Amely Campe , Bahne Christiansen , Akinyemi M. Fasemore , Silke F. Fischer , Martin Ganter , Sophia Körner , Gustavo R. Makert , Svea Matthiesen , Katja Mertens-Scholz , Sven Rinkel , Martin Runge , Jan Schulze-Luehrmann , Sebastian Ulbert , Fenja Winter , Dimitrios Frangoulidis , Anja Lührmann

The Q-GAPS (Q fever GermAn interdisciplinary Program for reSearch) consortium was launched in 2017 as a German consortium of more than 20 scientists with exceptional expertise, competence, and substantial knowledge in the field of the Q fever pathogen Coxiella (C.) burnetii. C. burnetii exemplifies as a zoonotic pathogen the challenges of zoonotic disease control and prophylaxis in human, animal, and environmental settings in a One Health approach. An interdisciplinary approach to studying the pathogen is essential to address unresolved questions about the epidemiology, immunology, pathogenesis, surveillance, and control of C. burnetii. In more than five years, Q-GAPS has provided new insights into pathogenicity and interaction with host defense mechanisms. The consortium has also investigated vaccine efficacy and application in animal reservoirs and identified expanded phenotypic and genotypic characteristics of C. burnetii and their epidemiological significance. In addition, conceptual principles for controlling, surveilling, and preventing zoonotic Q fever infections were developed and prepared for specific target groups. All findings have been continuously integrated into a Web-based, interactive, freely accessible knowledge and information platform (www.q-gaps.de), which also contains Q fever guidelines to support public health institutions in controlling and preventing Q fever. In this review, we will summarize our results and show an example of how an interdisciplinary consortium provides knowledge and better tools to control a zoonotic pathogen at the national level.

Q-GAPS(Q热德国跨学科再研究计划)联盟于2017年成立,是一个由20多位在Q热病原体烧伤柯西氏菌(C. Burnetii)领域具有卓越专长、能力和丰富知识的科学家组成的德国联盟。作为一种人畜共患病病原体,烧伤弧菌体现了在人类、动物和环境环境中以 "一种健康 "方法控制和预防人畜共患病所面临的挑战。要解决烧伤弧菌的流行病学、免疫学、发病机制、监测和控制等方面尚未解决的问题,就必须采用跨学科方法来研究这种病原体。在五年多的时间里,Q-GAPS 对致病性以及与宿主防御机制的相互作用有了新的认识。该研究小组还调查了疫苗的功效和在动物库中的应用,并确定了烧伤弧菌的更多表型和基因型特征及其流行病学意义。此外,还制定了控制、监测和预防人畜共患病 Q 热感染的概念原则,并为特定目标群体做了准备。所有研究结果都被不断整合到一个基于网络、交互式、可免费访问的知识和信息平台(www.q-gaps.de)中,该平台还包含Q热指南,以支持公共卫生机构控制和预防Q热。在本综述中,我们将总结我们的成果,并举例说明跨学科联盟如何提供知识和更好的工具,在国家层面控制人畜共患病。
{"title":"Interdisciplinary studies on Coxiella burnetii: From molecular to cellular, to host, to one health research","authors":"Benjamin U. Bauer ,&nbsp;Michael R. Knittler ,&nbsp;Jennifer Andrack ,&nbsp;Christian Berens ,&nbsp;Amely Campe ,&nbsp;Bahne Christiansen ,&nbsp;Akinyemi M. Fasemore ,&nbsp;Silke F. Fischer ,&nbsp;Martin Ganter ,&nbsp;Sophia Körner ,&nbsp;Gustavo R. Makert ,&nbsp;Svea Matthiesen ,&nbsp;Katja Mertens-Scholz ,&nbsp;Sven Rinkel ,&nbsp;Martin Runge ,&nbsp;Jan Schulze-Luehrmann ,&nbsp;Sebastian Ulbert ,&nbsp;Fenja Winter ,&nbsp;Dimitrios Frangoulidis ,&nbsp;Anja Lührmann","doi":"10.1016/j.ijmm.2023.151590","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151590","url":null,"abstract":"<div><p>The Q-GAPS (Q fever GermAn interdisciplinary Program for reSearch) consortium was launched in 2017 as a German consortium of more than 20 scientists with exceptional expertise, competence, and substantial knowledge in the field of the Q fever pathogen <em>Coxiella</em> (<em>C</em>.) <em>burnetii</em>. <em>C. burnetii</em> exemplifies as a zoonotic pathogen the challenges of zoonotic disease control and prophylaxis in human, animal, and environmental settings in a One Health approach. An interdisciplinary approach to studying the pathogen is essential to address unresolved questions about the epidemiology, immunology, pathogenesis, surveillance, and control of <em>C. burnetii</em>. In more than five years, Q-GAPS has provided new insights into pathogenicity and interaction with host defense mechanisms. The consortium has also investigated vaccine efficacy and application in animal reservoirs and identified expanded phenotypic and genotypic characteristics of <em>C. burnetii</em> and their epidemiological significance. In addition, conceptual principles for controlling, surveilling, and preventing zoonotic Q fever infections were developed and prepared for specific target groups. All findings have been continuously integrated into a Web-based, interactive, freely accessible knowledge and information platform (<span>www.q-gaps.de</span><svg><path></path></svg>), which also contains Q fever guidelines to support public health institutions in controlling and preventing Q fever. In this review, we will summarize our results and show an example of how an interdisciplinary consortium provides knowledge and better tools to control a zoonotic pathogen at the national level.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151590"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000188/pdfft?md5=b5ce7695402ae66210e9e93ff83c046a&pid=1-s2.0-S1438422123000188-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138489813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ListiWiki: A database for the foodborne pathogen Listeria monocytogenes ListiWiki:食源性单核细胞增生李斯特菌数据库
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.1016/j.ijmm.2023.151591
Christoph Elfmann , Bingyao Zhu , Jörg Stülke , Sven Halbedel

Listeria monocytogenes is a Gram positive foodborne pathogen that regularly causes outbreaks of systemic infectious diseases. The bacterium maintains a facultative intracellular lifestyle; it thrives under a variety of environmental conditions and is able to infect human host cells. L. monocytogenes is genetically tractable and therefore has become an attractive model system to study the mechanisms employed by facultative intracellular bacteria to invade eukaryotic cells and to replicate in their cytoplasm. Besides its importance for basic research, L. monocytogenes also serves as a paradigmatic pathogen in genomic epidemiology, where the relative stability of its genome facilitates successful outbreak detection and elucidation of transmission chains in genomic pathogen surveillance systems. In both terms, it is necessary to keep the annotation of the L. monocytogenes genome up to date. Therefore, we have created the database ListiWiki (http://listiwiki.uni-goettingen.de/) which stores comprehensive information on the widely used L. monocytogenes reference strain EDG-e. ListiWiki is designed to collect information on genes, proteins and RNAs and their relevant functional characteristics, but also further information such as mutant phenotypes, available biological material, and publications. In its present form, ListiWiki combines the most recent annotation of the EDG-e genome with published data on gene essentiality, gene expression and subcellular protein localization. ListiWiki also predicts protein-protein interactions networks based on protein homology to Bacillus subtilis proteins, for which detailed interaction maps have been compiled in the sibling database SubtiWiki. Furthermore, crystallographic information of proteins is made accessible through integration of Protein Structure Database codes and AlphaFold structure predictions. ListiWiki is an easy-to-use web interface that has been developed with a focus on an intuitive access to all information. Use of ListiWiki is free of charge and its content can be edited by all members of the scientific community after registration. In our labs, ListiWiki has already become an important and easy to use tool to quickly access genome annotation details that we can keep updated with advancing knowledge. It also might be useful to promote the comprehensive understanding of the physiology and virulence of an important human pathogen.

单核增生李斯特菌是一种革兰氏阳性食源性病原体,经常引起全身性传染病的暴发。细菌保持兼性的细胞内生活方式;它在各种环境条件下茁壮成长,并能够感染人类宿主细胞。单核增生乳杆菌具有遗传可塑性,因此成为研究兼性胞内细菌侵入真核细胞并在细胞质中复制机制的一个有吸引力的模型系统。除了对基础研究的重要性外,单核增生乳杆菌在基因组流行病学中也是一种典型的病原体,其基因组的相对稳定性有助于在基因组病原体监测系统中成功发现疫情和阐明传播链。在这两个方面,保持单核增生乳杆菌基因组的注释是必要的。因此,我们创建了数据库ListiWiki (http://listiwiki.uni-goettingen.de/),其中存储了广泛使用的单核增生乳杆菌参考菌株EDG-e的全面信息。ListiWiki旨在收集有关基因,蛋白质和rna及其相关功能特征的信息,以及诸如突变表型,可用生物材料和出版物等进一步信息。目前,ListiWiki将EDG-e基因组的最新注释与已发表的基因重要性、基因表达和亚细胞蛋白定位数据结合在一起。ListiWiki还预测了基于枯草芽孢杆菌蛋白质同源性的蛋白质-蛋白质相互作用网络,详细的相互作用图已经在兄弟数据库SubtiWiki中编译。此外,通过整合蛋白质结构数据库代码和AlphaFold结构预测,可以获得蛋白质的晶体学信息。ListiWiki是一个易于使用的web界面,它的开发重点是直观地访问所有信息。使用ListiWiki是免费的,注册后,所有科学界成员都可以编辑其内容。在我们的实验室中,ListiWiki已经成为一个重要且易于使用的工具,可以快速访问基因组注释细节,我们可以随着知识的进步而保持更新。这也可能有助于促进对一种重要人类病原体的生理和毒力的全面了解。
{"title":"ListiWiki: A database for the foodborne pathogen Listeria monocytogenes","authors":"Christoph Elfmann ,&nbsp;Bingyao Zhu ,&nbsp;Jörg Stülke ,&nbsp;Sven Halbedel","doi":"10.1016/j.ijmm.2023.151591","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151591","url":null,"abstract":"<div><p><em>Listeria monocytogenes</em> is a Gram positive foodborne pathogen that regularly causes outbreaks of systemic infectious diseases. The bacterium maintains a facultative intracellular lifestyle; it thrives under a variety of environmental conditions and is able to infect human host cells. <em>L. monocytogenes</em> is genetically tractable and therefore has become an attractive model system to study the mechanisms employed by facultative intracellular bacteria to invade eukaryotic cells and to replicate in their cytoplasm. Besides its importance for basic research, <em>L. monocytogenes</em> also serves as a paradigmatic pathogen in genomic epidemiology, where the relative stability of its genome facilitates successful outbreak detection and elucidation of transmission chains in genomic pathogen surveillance systems. In both terms, it is necessary to keep the annotation of the <em>L. monocytogenes</em> genome up to date. Therefore, we have created the database <em>Listi</em>Wiki (http://listiwiki.uni-goettingen.de/) which stores comprehensive information on the widely used <em>L. monocytogenes</em> reference strain EDG-e. <em>Listi</em>Wiki is designed to collect information on genes, proteins and RNAs and their relevant functional characteristics, but also further information such as mutant phenotypes, available biological material, and publications. In its present form, <em>Listi</em>Wiki combines the most recent annotation of the EDG-e genome with published data on gene essentiality, gene expression and subcellular protein localization. <em>Listi</em>Wiki also predicts protein-protein interactions networks based on protein homology to <em>Bacillus subtilis</em> proteins, for which detailed interaction maps have been compiled in the sibling database <em>Subti</em>Wiki. Furthermore, crystallographic information of proteins is made accessible through integration of Protein Structure Database codes and AlphaFold structure predictions. <em>Listi</em>Wiki is an easy-to-use web interface that has been developed with a focus on an intuitive access to all information. Use of <em>Listi</em>Wiki is free of charge and its content can be edited by all members of the scientific community after registration. In our labs, <em>Listi</em>Wiki has already become an important and easy to use tool to quickly access genome annotation details that we can keep updated with advancing knowledge. It also might be useful to promote the comprehensive understanding of the physiology and virulence of an important human pathogen.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151591"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S143842212300019X/pdfft?md5=84ba6edb81061c002eeb42efac77a618&pid=1-s2.0-S143842212300019X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138474161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections BioFire JI小组的多中心评估与改善急性骨关节感染的微生物学诊断有关。
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.1016/j.ijmm.2023.151588
Llanos Salar-Vidal , Catarina Chaves , Ileana T. Dianzo-Delgado , Patricio Favier , Salvador Giner-Almaraz , María José Gómez-Gómez , Guillermo Martín-Gutiérrez , Isabel Pereira , Ana Rodríguez-Fernández , Patricia Ruiz-Garbajosa , Carlos Salas-Venero , Jaime Esteban

Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as K. kingae and N. gonorrhoeae. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.

骨关节感染(OI)的微生物学诊断对于成功的治疗至关重要。2021年7月至2022年10月进行了一项前瞻性多中心研究,包括262种疑似急性OI的滑膜液。进行BioFire关节感染小组多重PCR检测,并将结果与滑膜液标本的传统培养物进行比较。总共检测到136种微生物,14个样本的一种以上微生物呈阳性。在单菌感染(n=87)中,与培养的一致性为69%。在26个样品中,当培养结果为阴性时,多重PCR产生额外的阳性结果。它有助于检测挑剔的微生物,如金黄色葡萄球菌和淋球菌。这种多重PCR已被证明是一种有用的技术,可用于高度怀疑急性OI的患者,以快速和自动化的方式。
{"title":"Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections","authors":"Llanos Salar-Vidal ,&nbsp;Catarina Chaves ,&nbsp;Ileana T. Dianzo-Delgado ,&nbsp;Patricio Favier ,&nbsp;Salvador Giner-Almaraz ,&nbsp;María José Gómez-Gómez ,&nbsp;Guillermo Martín-Gutiérrez ,&nbsp;Isabel Pereira ,&nbsp;Ana Rodríguez-Fernández ,&nbsp;Patricia Ruiz-Garbajosa ,&nbsp;Carlos Salas-Venero ,&nbsp;Jaime Esteban","doi":"10.1016/j.ijmm.2023.151588","DOIUrl":"10.1016/j.ijmm.2023.151588","url":null,"abstract":"<div><p>Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as <em>K. kingae</em> and <em>N. gonorrhoeae</em>. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151588"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71488264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association between hospital onset of infection and outcomes in sepsis patients – A propensity score matched cohort study based on health claims data in Germany 医院感染发病与败血症患者预后之间的关系——基于德国健康声明数据的倾向评分匹配队列研究
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.1016/j.ijmm.2023.151593
Norman Rose , Melissa Spoden , Antje Freytag , Mathias Pletz , Tim Eckmanns , Lisa Wedekind , Josephine Storch , Peter Schlattmann , Christiane S. Hartog , Konrad Reinhart , Christian Günster , Carolin Fleischmann-Struzek

Background

Hospital-acquired infections are a common source of sepsis. Hospital onset of sepsis was found to be associated with higher acute mortality and hospital costs, yet its impact on long-term patient-relevant outcomes and costs is unknown.

Objective

We aimed to assess the association between sepsis origin and acute and long-term outcomes based on a nationwide population-based cohort of sepsis patients in Germany.

Methods

This retrospective cohort study used nationwide health claims data from 23 million health insurance beneficiaries. Sepsis patients with hospital-acquired infections (HAI) were identified by ICD-10-codes in a cohort of adult patients with hospital-treated sepsis between 2013 and 2014. Cases without these ICD-10-codes were considered as sepsis cases with community-acquired infection (CAI) and were matched with HAI sepsis patients by propensity score matching. Outcomes included in-hospital/12-month mortality and costs, as well as readmissions and nursing care dependency until 12 months postsepsis.

Results

We matched 33,110 HAI sepsis patients with 28,614 CAI sepsis patients and 22,234 HAI sepsis hospital survivors with 19,364 CAI sepsis hospital survivors. HAI sepsis patients had a higher hospital mortality than CAI sepsis patients (32.8% vs. 25.4%, RR 1.3, p < .001). Similarly, 12-months postacute mortality was higher (37.2% vs. 30.1%, RR=1.2, p < .001). Hospital and 12-month health care costs were 178% and 22% higher in HAI patients than in CAI patients, respectively. Twelve months postsepsis, HAI sepsis survivors were more often newly dependent on nursing care (33.4% vs. 24.0%, RR=1.4, p < .001) and experienced 5% more hospital readmissions (mean number of readmissions: 2.1 vs. 2.0, p < .001).

Conclusions

HAI sepsis patients face an increased risk of adverse outcomes both during the acute sepsis episode and in the long-term. Measures to prevent HAI and its progression into sepsis may be an opportunity to mitigate the burden of long-term impairments and costs of sepsis, e.g., by early detection of HAI progressing into sepsis, particularly in normal wards; adequate sepsis management and adherence to sepsis bundles in hospital-acquired sepsis; and an improved infection prevention and control.

背景:医院获得性感染是脓毒症的常见来源。医院发生败血症与较高的急性死亡率和住院费用相关,但其对患者相关的长期结局和费用的影响尚不清楚。目的:基于德国一项全国性的脓毒症患者队列研究,我们旨在评估脓毒症起源与急性和长期预后之间的关系。方法:本回顾性队列研究使用了全国2300万健康保险受益人的健康索赔数据。2013年至2014年期间,通过icd -10代码在一组住院治疗的脓毒症成人患者中鉴定出伴有医院获得性感染(HAI)的脓毒症患者。没有这些icd -10编码的病例被认为是脓毒症合并社区获得性感染(CAI),并通过倾向评分匹配与HAI脓毒症患者进行匹配。结果包括住院/12个月死亡率和费用,以及再入院率和护理依赖性,直到败血症后12个月。结果33,110例HAI败血症患者与28,614例CAI败血症患者相匹配,22,234例HAI败血症医院幸存者与19,364例CAI败血症医院幸存者相匹配。HAI脓毒症患者的住院死亡率高于CAI脓毒症患者(32.8% vs. 25.4%, RR 1.3, p<.001)。同样,急性后12个月死亡率更高(37.2% vs. 30.1%, RR=1.2, p< 0.001)。HAI患者住院和12个月的医疗费用分别比CAI患者高178%和22%。脓毒症后12个月,HAI脓毒症幸存者更经常依赖护理(33.4%比24.0%,RR=1.4, p < 0.01),再入院率高出5%(平均再入院次数:2.1比2.0,p < 0.01)。结论shai脓毒症患者无论是在急性脓毒症发作期间还是在长期内,不良结局的风险都有所增加。预防HAI及其进展为败血症的措施可能是减轻长期损害负担和败血症费用的机会,例如,通过早期发现进展为败血症的HAI,特别是在正常病房;在医院获得性败血症中,适当的败血症管理和遵守败血症包;改善感染预防和控制。
{"title":"Association between hospital onset of infection and outcomes in sepsis patients – A propensity score matched cohort study based on health claims data in Germany","authors":"Norman Rose ,&nbsp;Melissa Spoden ,&nbsp;Antje Freytag ,&nbsp;Mathias Pletz ,&nbsp;Tim Eckmanns ,&nbsp;Lisa Wedekind ,&nbsp;Josephine Storch ,&nbsp;Peter Schlattmann ,&nbsp;Christiane S. Hartog ,&nbsp;Konrad Reinhart ,&nbsp;Christian Günster ,&nbsp;Carolin Fleischmann-Struzek","doi":"10.1016/j.ijmm.2023.151593","DOIUrl":"10.1016/j.ijmm.2023.151593","url":null,"abstract":"<div><h3>Background</h3><p>Hospital-acquired infections are a common source of sepsis. Hospital onset of sepsis was found to be associated with higher acute mortality and hospital costs, yet its impact on long-term patient-relevant outcomes and costs is unknown.</p></div><div><h3>Objective</h3><p>We aimed to assess the association between sepsis origin and acute and long-term outcomes based on a nationwide population-based cohort of sepsis patients in Germany.</p></div><div><h3>Methods</h3><p>This retrospective cohort study used nationwide health claims data from 23 million health insurance beneficiaries. Sepsis patients with hospital-acquired infections (HAI) were identified by ICD-10-codes in a cohort of adult patients with hospital-treated sepsis between 2013 and 2014. Cases without these ICD-10-codes were considered as sepsis cases with community-acquired infection (CAI) and were matched with HAI sepsis patients by propensity score matching. Outcomes included in-hospital/12-month mortality and costs, as well as readmissions and nursing care dependency until 12 months postsepsis.</p></div><div><h3>Results</h3><p>We matched 33,110 HAI sepsis patients with 28,614 CAI sepsis patients and 22,234 HAI sepsis hospital survivors with 19,364 CAI sepsis hospital survivors. HAI sepsis patients had a higher hospital mortality than CAI sepsis patients (32.8% vs. 25.4%, RR 1.3, p &lt; .001). Similarly, 12-months postacute mortality was higher (37.2% vs. 30.1%, RR=1.2, p &lt; .001). Hospital and 12-month health care costs were 178% and 22% higher in HAI patients than in CAI patients, respectively. Twelve months postsepsis, HAI sepsis survivors were more often newly dependent on nursing care (33.4% vs. 24.0%, RR=1.4, p &lt; .001) and experienced 5% more hospital readmissions (mean number of readmissions: 2.1 vs. 2.0, p &lt; .001).</p></div><div><h3>Conclusions</h3><p>HAI sepsis patients face an increased risk of adverse outcomes both during the acute sepsis episode and in the long-term. Measures to prevent HAI and its progression into sepsis may be an opportunity to mitigate the burden of long-term impairments and costs of sepsis, e.g., by early detection of HAI progressing into sepsis, particularly in normal wards; adequate sepsis management and adherence to sepsis bundles in hospital-acquired sepsis; and an improved infection prevention and control.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151593"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000218/pdfft?md5=cc7369e9bf20126c308b4a6ce79bc5c4&pid=1-s2.0-S1438422123000218-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of resveratrol on macrophages after phagocytosis of Candida glabrata 白藜芦醇对裸念珠菌吞噬后巨噬细胞的影响。
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.1016/j.ijmm.2023.151589
Zong-Han Chen , Meng Guan , Wei-Jia Zhao

Candida glabrata is believed to be the underlying cause of many human ailments, including oral, gastrointestinal, and vaginal disorders. C. glabrata-caused deep-seated infections, coupled with its resistance to antifungal drugs, may contribute to a high mortality rate. Resveratrol is a polyphenol and can achieve better therapeutic effects when administered in combination with micafungin, but the underlying molecular mechanisms remain unknown. Here, we investigate the effects of varying doses of resveratrol on the proliferation, apoptosis, and activity of macrophages, which were co-cultured with micafungin-pretreated C. glabrata. Resveratrol can restore the decreased proliferative activity of macrophages caused by the phagocytosis of C. glabrata. Further investigations demonstrated that this restoration ability exhibited a dose-dependent manner, reaching the highest level at 200 µM of resveratrol. Resveratrol tended to be more effective in inhibiting macrophage apoptosis and reducing reactive oxygen species (ROS) levels with concentration increases. In addition, at medium concentrations, resveratrol may down-regulate the expression of most inflammatory cytokines, whereas at high concentrations, it started to exert pro-inflammatory functions by up-regulating their expressions. Macrophages may shift from an anti-inflammatory (M2) phenotype to an inflammatory (M1) phenotype by resveratrol at 200 µM, and from M1 to M2 at 400 µM. Our research shows that resveratrol with micafungin are effective in treating C. glabrata infections. The resveratrol-micafungin combination can reduce the production of ROS, and promote the proliferation, inhibit the apoptosis, and activate the polarization of macrophages in a dose-dependent manner. This study offers insights into how this combination works and may provide possible direction for further clinical application of the combination.

光念珠菌被认为是许多人类疾病的潜在原因,包括口腔、胃肠道和阴道疾病。光齿梭菌引起的深层感染,加上其对抗真菌药物的耐药性,可能导致高死亡率。白藜芦醇是一种多酚,与米卡芬宁联合使用可获得更好的治疗效果,但其潜在的分子机制尚不清楚。在这里,我们研究了不同剂量的白藜芦醇对巨噬细胞增殖、凋亡和活性的影响,巨噬细胞与micafunin预处理的C. glabrata共培养。白藜芦醇能恢复巨噬细胞因巨噬细胞吞噬作用而降低的增殖活性。进一步的研究表明,这种恢复能力表现出剂量依赖性,在200µM白藜芦醇时达到最高水平。随着白藜芦醇浓度的增加,其抑制巨噬细胞凋亡和降低活性氧(ROS)水平的效果更明显。此外,在中等浓度下,白藜芦醇可以下调大多数炎症细胞因子的表达,而在高浓度下,白藜芦醇通过上调其表达开始发挥促炎功能。在200µM白藜芦醇作用下,巨噬细胞可从抗炎(M2)表型转变为炎症(M1)表型,在400µM白藜芦醇作用下,巨噬细胞可从M1表型转变为M2表型。我们的研究表明,白藜芦醇与米卡芬宁联合治疗光秃锥体感染是有效的。白藜芦醇-米卡芬金联合用药可减少ROS的产生,促进巨噬细胞增殖,抑制细胞凋亡,激活巨噬细胞极化,并呈剂量依赖性。本研究提供了该组合如何工作的见解,并可能为该组合的进一步临床应用提供可能的方向。
{"title":"Effects of resveratrol on macrophages after phagocytosis of Candida glabrata","authors":"Zong-Han Chen ,&nbsp;Meng Guan ,&nbsp;Wei-Jia Zhao","doi":"10.1016/j.ijmm.2023.151589","DOIUrl":"10.1016/j.ijmm.2023.151589","url":null,"abstract":"<div><p><em>Candida glabrata</em> is believed to be the underlying cause of many human ailments, including oral, gastrointestinal, and vaginal disorders. <em>C. glabrata</em>-caused deep-seated infections, coupled with its resistance to antifungal drugs, may contribute to a high mortality rate. Resveratrol is a polyphenol and can achieve better therapeutic effects when administered in combination with micafungin, but the underlying molecular mechanisms remain unknown. Here, we investigate the effects of varying doses of resveratrol on the proliferation, apoptosis, and activity of macrophages, which were co-cultured with micafungin-pretreated <em>C. glabrata</em>. Resveratrol can restore the decreased proliferative activity of macrophages caused by the phagocytosis of <em>C. glabrata</em>. Further investigations demonstrated that this restoration ability exhibited a dose-dependent manner, reaching the highest level at 200 µM of resveratrol. Resveratrol tended to be more effective in inhibiting macrophage apoptosis and reducing reactive oxygen species (ROS) levels with concentration increases. In addition, at medium concentrations, resveratrol may down-regulate the expression of most inflammatory cytokines, whereas at high concentrations, it started to exert pro-inflammatory functions by up-regulating their expressions. Macrophages may shift from an anti-inflammatory (M2) phenotype to an inflammatory (M1) phenotype by resveratrol at 200 µM, and from M1 to M2 at 400 µM. Our research shows that resveratrol with micafungin are effective in treating <em>C. glabrata</em> infections. The resveratrol-micafungin combination can reduce the production of ROS, and promote the proliferation, inhibit the apoptosis, and activate the polarization of macrophages in a dose-dependent manner. This study offers insights into how this combination works and may provide possible direction for further clinical application of the combination.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151589"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000176/pdfft?md5=10c10801df9fb875840599bbe8fa302a&pid=1-s2.0-S1438422123000176-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polydatin alleviates mycoplasma pneumoniae-induced injury via inhibition of Caspase-1/GSDMD-dependent pyroptosis Polydatin通过抑制Caspase1/GSDMD依赖性pyroptosis减轻肺炎支原体诱导的损伤。
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-09-01 DOI: 10.1016/j.ijmm.2023.151586
Yiliu Chen , Yonghong Jiang , Xiuxiu Liu, Xiufeng Chen, Qiuyue Fan, Zhen Xiao

Mycoplasma pneumoniae (MP) is one of the main pathogens causing community acquired pneumonia (CAP) in children and adults. Previous pharmacological and clinical studies have shown that Polydatin (PD) exerts anti-inflammatory action by conferring protective benefit in MP pneumonia. However, the mechanism underlying the of PD on MP infection remains unclear. It was found that PD alleviated MP-induced injury by inhibiting caspase-1/gasdermin D (GSDMD)-mediated epithelial pyroptosis. The results demonstrated that PD inhibited the transformation of GSDMD to N-terminal gasdermin-N (GSDMD-N) by decreasing caspase-1 activation, as well as suppressed the formation and secretion of interleukin-1β (IL-1β) and interleukin-18 (IL-18), reversed Na, K-ATPase reduction, and suppressed LDH release both in vitro and vivo. Taken together, epithelial pyroptosis in BEAS‐2B cells and lung injury in mice were prevented by PD. In conclusion, PD suppressed pulmonary injury triggered by MP infection, by inhibiting the caspase-1/GSDMD-mediated epithelial pyroptosis signaling pathway. Thus, PD may be regarded as a potential therapy for MP-induced inflammation.

肺炎支原体(MP)是引起儿童和成人社区获得性肺炎(CAP)的主要病原体之一。先前的药理学和临床研究表明,Polydatin(PD)通过对MP肺炎的保护作用发挥抗炎作用。然而,帕金森病对MP感染的潜在机制尚不清楚。研究发现,PD通过抑制胱天蛋白酶1/gasdermin D(GSDMD)介导的上皮焦下垂来减轻MP诱导的损伤。结果表明,PD通过降低胱天蛋白酶-1的激活来抑制GSDMD向N-末端gasdermin-N(GSDMD-N)的转化,并在体内外抑制白细胞介素-1β(IL-1β)和IL-18的形成和分泌,逆转Na、K-ATP酶的减少,抑制LDH的释放。总之,PD可以预防BEAS-2B细胞的上皮性pyroptosis和小鼠的肺损伤。总之,PD通过抑制胱天蛋白酶1/GSDMD介导的上皮性pyroptosis信号通路来抑制MP感染引发的肺损伤。因此,PD可被视为MP诱导炎症的潜在治疗方法。
{"title":"Polydatin alleviates mycoplasma pneumoniae-induced injury via inhibition of Caspase-1/GSDMD-dependent pyroptosis","authors":"Yiliu Chen ,&nbsp;Yonghong Jiang ,&nbsp;Xiuxiu Liu,&nbsp;Xiufeng Chen,&nbsp;Qiuyue Fan,&nbsp;Zhen Xiao","doi":"10.1016/j.ijmm.2023.151586","DOIUrl":"10.1016/j.ijmm.2023.151586","url":null,"abstract":"<div><p>Mycoplasma pneumoniae (MP) is one of the main pathogens causing community acquired pneumonia (CAP) in children and adults. Previous pharmacological and clinical studies have shown that Polydatin (PD) exerts anti-inflammatory action by conferring protective benefit in MP pneumonia. However, the mechanism underlying the of PD on MP infection remains unclear. It was found that PD alleviated MP-induced injury by inhibiting caspase-1/gasdermin D (GSDMD)-mediated epithelial pyroptosis. The results demonstrated that PD inhibited the transformation of GSDMD to N-terminal gasdermin-N (GSDMD-N) by decreasing caspase-1 activation, as well as suppressed the formation and secretion of interleukin-1β (IL-1β) and interleukin-18 (IL-18), reversed Na, K-ATPase reduction, and suppressed LDH release both in vitro and vivo. Taken together, epithelial pyroptosis in BEAS‐2B cells and lung injury in mice were prevented by PD. In conclusion, PD suppressed pulmonary injury triggered by MP infection, by inhibiting the caspase-1/GSDMD-mediated epithelial pyroptosis signaling pathway. Thus, PD may be regarded as a potential therapy for MP-induced inflammation.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 5","pages":"Article 151586"},"PeriodicalIF":4.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41179181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenotypic and genotypic discrimination of Francisella tularensis ssp. holarctica clades 土拉菌的表型和基因型鉴定。holarctica演化支
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-07-01 DOI: 10.1016/j.ijmm.2023.151583
Kristin Köppen , Kerstin Rydzewski , Joerg Doellinger , Kerstin Myrtennäs , Mats Forsman , Sandra Appelt , Holger Scholz , Klaus Heuner

Francisella tularensis is the causative agent of tularemia, a zoonotic disease with a wide host range. F. tularensis ssp. holarctica (Fth) is of clinical relevance for European countries, including Germany. Whole genome sequencing methods, including canonical Single Nucleotide Polymorphism (canSNP) typing and whole genome SNP typing, have revealed that European Fth strains belong to a few monophyletic populations. The majority of German Fth isolates belong to two basal phylogenetic clades B.6 (biovar I) and B.12 (biovar II). Strains of B.6 and B.12 seem to differ in their pathogenicity, and it has been shown that strains of biovar II are resistant against erythromycin. In this study, we present data corroborating our previous data demonstrating that basal clade B.12 can be divided into clades B.71 and B.72. By applying phylogenetic whole genome analysis as well as proteome analysis, we could verify that strains of these two clades are distinct from one another. This was confirmed by measuring the intensity of backscatter light on bacteria grown in liquid media. Strains belonging to clades B.6, B.71 or B.72 showed clade-specific backscatter growth curves. Furthermore, we present the whole genome sequence of strain A-1341, as a reference genome of clade B.71, and whole proteomes comparison of Fth strains belonging to clades B.6, B.71 and B.72. Further research is necessary to investigate phenotypes and putative differences in pathogenicity of the investigated different clades of Fth to better understand the relationship between observed phenotypes, pathogenicity and distribution of Fth strains.

兔热病是一种宿主范围广泛的人畜共患疾病。F.tularensis ssp。holarctica(Fth)在包括德国在内的欧洲国家具有临床相关性。全基因组测序方法,包括标准单核苷酸多态性(canSNP)分型和全基因组SNP分型,表明欧洲Fth菌株属于少数单系群体。大多数德国Fth分离株属于两个基础系统发育分支B.6(生物型I)和B.12(生物型II)。B.6和B.12菌株的致病性似乎不同,并且已经表明生物型II菌株对红霉素具有抗性。在这项研究中,我们提供的数据证实了我们之前的数据,表明基础分支B.12可以分为分支B.71和B.72。通过应用系统发育全基因组分析和蛋白质组分析,我们可以验证这两个分支的菌株彼此不同。这是通过测量在液体培养基中生长的细菌的反向散射光的强度来证实的。属于分支B.6、B.71或B.72的菌株显示出分支特异性反向散射生长曲线。此外,我们提供了菌株A-1341的全基因组序列,作为分支B.71的参考基因组,以及属于分支B.6、B.71和B.72的Fth菌株的全蛋白质组比较。有必要进一步研究Fth不同分支的表型和致病性的假定差异,以更好地了解观察到的表型、致病性和Fth菌株分布之间的关系。
{"title":"Phenotypic and genotypic discrimination of Francisella tularensis ssp. holarctica clades","authors":"Kristin Köppen ,&nbsp;Kerstin Rydzewski ,&nbsp;Joerg Doellinger ,&nbsp;Kerstin Myrtennäs ,&nbsp;Mats Forsman ,&nbsp;Sandra Appelt ,&nbsp;Holger Scholz ,&nbsp;Klaus Heuner","doi":"10.1016/j.ijmm.2023.151583","DOIUrl":"10.1016/j.ijmm.2023.151583","url":null,"abstract":"<div><p><em>Francisella tularensis</em> is the causative agent of tularemia, a zoonotic disease with a wide host range. <em>F. tularensis</em> ssp. <em>holarctica</em> (<em>Fth</em>) is of clinical relevance for European countries, including Germany. Whole genome sequencing methods, including canonical Single Nucleotide Polymorphism (canSNP) typing and whole genome SNP typing, have revealed that European <em>Fth</em> strains belong to a few monophyletic populations. The majority of German <em>Fth</em> isolates belong to two basal phylogenetic clades B.6 (biovar I) and B.12 (biovar II). Strains of B.6 and B.12 seem to differ in their pathogenicity, and it has been shown that strains of biovar II are resistant against erythromycin. In this study, we present data corroborating our previous data demonstrating that basal clade B.12 can be divided into clades B.71 and B.72. By applying phylogenetic whole genome analysis as well as proteome analysis, we could verify that strains of these two clades are distinct from one another. This was confirmed by measuring the intensity of backscatter light on bacteria grown in liquid media. Strains belonging to clades B.6, B.71 or B.72 showed clade-specific backscatter growth curves. Furthermore, we present the whole genome sequence of strain A-1341, as a reference genome of clade B.71, and whole proteomes comparison of <em>Fth</em> strains belonging to clades B.6, B.71 and B.72. Further research is necessary to investigate phenotypes and putative differences in pathogenicity of the investigated different clades of <em>Fth</em> to better understand the relationship between observed phenotypes, pathogenicity and distribution of <em>Fth</em> strains.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 4","pages":"Article 151583"},"PeriodicalIF":4.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9972997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Innate activation of human neutrophils and neutrophil-like cells by the pro-inflammatory bacterial metabolite ADP-heptose and Helicobacter pylori 促炎细菌代谢产物ADP庚糖和幽门螺杆菌对人中性粒细胞和中性粒细胞样细胞的天然激活
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-07-01 DOI: 10.1016/j.ijmm.2023.151585
Larissa Faass , Martina Hauke , Saskia C. Stein , Christine Josenhans

Lipopolysaccharide inner core heptose metabolites, including ADP-heptose, play a substantial role in the activation of cell-autonomous innate immune responses in eukaryotic cells, via the ALPK1-TIFA signaling pathway, as demonstrated for various pathogenic bacteria. The important role of LPS heptose metabolites during Helicobacter pylori infection of the human gastric niche has been demonstrated for gastric epithelial cells and macrophages, while the role of heptose metabolites on human neutrophils has not been investigated. In this study, we aimed to gain a better understanding of the activation potential of bacterial heptose metabolites for human neutrophil cells. To do so, we used pure ADP-heptose and, as a bacterial model, H. pylori, which can transport heptose metabolites into the human host cell via the Cag Type 4 Secretion System (CagT4SS). Main questions were how bacterial heptose metabolites impact on the pro-inflammatory activation, alone and in the bacterial context, and how they influence maturation of human neutrophils. Results of the present study demonstrated that neutrophils respond with high sensitivity to pure heptose metabolites, and that global regulation networks and neutrophil maturation are influenced by heptose exposure. Furthermore, activation of human neutrophils by live H. pylori is strongly impacted by the presence of LPS heptose metabolites and the functionality of its CagT4SS. Similar activities were determined in cell culture neutrophils of different maturation states and in human primary neutrophils. In conclusion, we demonstrated that specific heptose metabolites or bacteria producing heptoses exhibit a strong activity on cell-autonomous innate responses of human neutrophils.

脂多糖内核庚糖代谢产物,包括ADP庚糖,通过ALPK1-TIFA信号通路,在真核细胞中激活细胞自主先天免疫反应中发挥重要作用,如对各种致病菌所证明的那样。LPS庚糖代谢产物在幽门螺杆菌感染人类胃生态位过程中的重要作用已被证明是针对胃上皮细胞和巨噬细胞的,而庚糖代谢物对人类中性粒细胞的作用尚未得到研究。在这项研究中,我们旨在更好地了解细菌庚糖代谢产物对人类中性粒细胞的激活潜力。为此,我们使用了纯ADP庚糖和幽门螺杆菌作为细菌模型,幽门螺杆菌可以通过Cag 4型分泌系统(CagT4SS)将庚糖代谢产物转运到人类宿主细胞中。主要问题是细菌庚糖代谢产物如何单独和在细菌环境中影响促炎激活,以及它们如何影响人类中性粒细胞的成熟。本研究的结果表明,中性粒细胞对纯庚糖代谢产物具有高度敏感性,并且全球调节网络和中性粒细胞成熟受到庚糖暴露的影响。此外,活幽门螺杆菌对人类中性粒细胞的激活受到LPS庚糖代谢产物及其CagT4SS功能的强烈影响。在不同成熟状态的细胞培养中性粒细胞和人类原代中性粒细胞中测定了类似的活性。总之,我们证明了特定的庚糖代谢产物或产生庚糖的细菌对人类中性粒细胞的细胞自主先天反应表现出强大的活性。
{"title":"Innate activation of human neutrophils and neutrophil-like cells by the pro-inflammatory bacterial metabolite ADP-heptose and Helicobacter pylori","authors":"Larissa Faass ,&nbsp;Martina Hauke ,&nbsp;Saskia C. Stein ,&nbsp;Christine Josenhans","doi":"10.1016/j.ijmm.2023.151585","DOIUrl":"10.1016/j.ijmm.2023.151585","url":null,"abstract":"<div><p>Lipopolysaccharide inner core heptose metabolites, including ADP-heptose, play a substantial role in the activation of cell-autonomous innate immune responses in eukaryotic cells, via the ALPK1-TIFA signaling pathway, as demonstrated for various pathogenic bacteria. The important role of LPS heptose metabolites during <em>Helicobacter pylori</em> infection of the human gastric niche has been demonstrated for gastric epithelial cells and macrophages, while the role of heptose metabolites on human neutrophils has not been investigated. In this study, we aimed to gain a better understanding of the activation potential of bacterial heptose metabolites for human neutrophil cells. To do so, we used pure ADP-heptose and, as a bacterial model, <em>H. pylori</em>, which can transport heptose metabolites into the human host cell via the Cag Type 4 Secretion System (CagT4SS). Main questions were how bacterial heptose metabolites impact on the pro-inflammatory activation, alone and in the bacterial context, and how they influence maturation of human neutrophils. Results of the present study demonstrated that neutrophils respond with high sensitivity to pure heptose metabolites, and that global regulation networks and neutrophil maturation are influenced by heptose exposure. Furthermore, activation of human neutrophils by live <em>H. pylori</em> is strongly impacted by the presence of LPS heptose metabolites and the functionality of its CagT4SS. Similar activities were determined in cell culture neutrophils of different maturation states and in human primary neutrophils. In conclusion, we demonstrated that specific heptose metabolites or bacteria producing heptoses exhibit a strong activity on cell-autonomous innate responses of human neutrophils.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 4","pages":"Article 151585"},"PeriodicalIF":4.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9977343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Heat shock protein 70 is involved in polaprezinc driven cell protection against Helicobacter pylori-induced injury 热休克蛋白70参与了polaprezinc驱动的细胞对幽门螺杆菌诱导的损伤的保护
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-05-01 DOI: 10.1016/j.ijmm.2023.151582
Fansen Meng , Siying Zhu , Meiliang Gong , Hongjin Tao , Weihua Wang , Gangshi Wang

Polaprezinc (PZ) plays a role in the protection of gastric mucosa and inhibiting Helicobacter pylori (H. pylori) growth in vitro. The objective of this study was to determine the protective effects of PZ on human gastric epithelial cells (GES-1) against H. pylori-induced damage, while also examining heat shock protein 70 (HSP70) as a potential underlying factor in this protection. Our findings revealed that PZ exerted bactericidal effects against H. pylori strains. We also observed that PZ mitigated the H. pylori-induced damage to GES-1 cells by increasing cell viability, reducing LDH release, and decreasing the secretion of pro-inflammatory factors such as MCP-1 and IL-6. Co-culturing PZ with GES-1 cells significantly up-regulated the GES-1 HSP70 expression in both a time and dose-dependent manner. Pre-incubating (for 12 h) or co-culturing (for 24 h) GES-1 cells with PZ reversed the down-regulation of HSP70 in GES-1 cells caused by H. pylori infection. However, when quercetin was used to inhibit the up-regulation of HSP70 in GES-1 cells, the protective effect of PZ on GES-1 cells was significantly reduced. Based on the results of this study, PZ exhibits a protective role on GES-1 cells against H. pylori injury, as well as a direct bactericidal effect on H. pylori. HSP70 is involved in the PZ-driven host cell protection against H. pylori injury. These findings provide insight into alternative strategies for H. pylori treatment.

吡嗪酮(PZ)在体外具有保护胃黏膜和抑制幽门螺杆菌(H.pylori)生长的作用。本研究的目的是确定PZ对人胃上皮细胞(GES-1)对幽门螺杆菌诱导的损伤的保护作用,同时检测热休克蛋白70(HSP70)作为这种保护的潜在潜在潜在因素。我们的研究结果表明PZ对幽门螺杆菌菌株具有杀菌作用。我们还观察到,PZ通过增加细胞活力、减少LDH释放和减少促炎因子如MCP-1和IL-6的分泌,减轻了幽门螺杆菌诱导的GES-1细胞损伤。PZ与GES-1细胞共培养以时间和剂量依赖的方式显著上调GES-1 HSP70的表达。预孵育(12小时)或与PZ共培养(24小时)GES-1细胞逆转了幽门螺杆菌感染引起的GES-1中HSP70的下调。然而,当槲皮素用于抑制GES-1细胞中HSP70的上调时,PZ对GES-1的保护作用显著降低。根据本研究的结果,PZ对GES-1细胞对幽门螺杆菌损伤具有保护作用,并对幽门螺杆杆菌具有直接杀菌作用。HSP70参与PZ驱动的宿主细胞对幽门螺杆菌损伤的保护。这些发现为幽门螺杆菌治疗的替代策略提供了见解。
{"title":"Heat shock protein 70 is involved in polaprezinc driven cell protection against Helicobacter pylori-induced injury","authors":"Fansen Meng ,&nbsp;Siying Zhu ,&nbsp;Meiliang Gong ,&nbsp;Hongjin Tao ,&nbsp;Weihua Wang ,&nbsp;Gangshi Wang","doi":"10.1016/j.ijmm.2023.151582","DOIUrl":"10.1016/j.ijmm.2023.151582","url":null,"abstract":"<div><p>Polaprezinc (PZ) plays a role in the protection of gastric mucosa and inhibiting <em>Helicobacter pylori</em> (<em>H. pylori</em>) growth in vitro. The objective of this study was to determine the protective effects of PZ on human gastric epithelial cells (GES-1) against <em>H. pylori</em>-induced damage, while also examining heat shock protein 70 (HSP70) as a potential underlying factor in this protection. Our findings revealed that PZ exerted bactericidal effects against <em>H. pylori</em> strains. We also observed that PZ mitigated the <em>H. pylori</em>-induced damage to GES-1 cells by increasing cell viability, reducing LDH release, and decreasing the secretion of pro-inflammatory factors such as MCP-1 and IL-6. Co-culturing PZ with GES-1 cells significantly up-regulated the GES-1 HSP70 expression in both a time and dose-dependent manner. Pre-incubating (for 12 h) or co-culturing (for 24 h) GES-1 cells with PZ reversed the down-regulation of HSP70 in GES-1 cells caused by <em>H. pylori</em> infection. However, when quercetin was used to inhibit the up-regulation of HSP70 in GES-1 cells, the protective effect of PZ on GES-1 cells was significantly reduced. Based on the results of this study, PZ exhibits a protective role on GES-1 cells against <em>H. pylori</em> injury, as well as a direct bactericidal effect on <em>H. pylori</em>. HSP70 is involved in the PZ-driven host cell protection against <em>H. pylori</em> injury. These findings provide insight into alternative strategies for <em>H. pylori</em> treatment.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151582"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10176086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Longitudinal relationship between the gut microbiota variation and diversity and gut graft-versus-host disease (GVHD) following pediatric allogeneic hematopoietic cell transplantation (HCT) – Case series 儿童异基因造血细胞移植(HCT)后肠道微生物群变异和多样性与肠道移植物抗宿主病(GVHD)之间的纵向关系——病例系列
IF 4.1 3区 医学 Q1 MICROBIOLOGY Pub Date : 2023-05-01 DOI: 10.1016/j.ijmm.2023.151580
Ashley N. Gray , Nicole H. Tobin , Theodore B. Moore , Fan Li , Grace M. Aldrovandi

Allogeneic Hematopoietic Cell Transplantation (HCT) offers children with life-threatening diseases a chance at survival. Complications from graft-versus-host disease (GVHD, Stages 0–4) represent a significant cause of morbidity and mortality which has been recently associated with gut dysbiosis the adult HCT population. Here, our objective was to conduct a prospective, longitudinal cohort study in nine pediatric allogeneic HCT participants by collecting longitudinally post-HCT stool specimens up to 1 year. Stool microbiota analyses showed that allogeneic HCT and antibiotic therapy lead to acute shifts in the diversity of the gut microbiota with those experiencing stages 3–4 gut GVHD having significantly greater microbiota variation over time when compared to control participants (p = 0.007). Pre-HCT microbiota diversity trended towards an inverse relationship with gut microbiota stability over time, however, this did not reach statistical significance (p = 0.05). Future large prospective studies are necessary to elucidate the mechanisms underlying these dynamic changes in the gut microbiota following pediatric allogeneic HCT.

异基因造血细胞移植为患有危及生命的疾病的儿童提供了生存的机会。移植物抗宿主病(GVHD,0-4期)的并发症是发病率和死亡率的重要原因,最近与成年HCT人群的肠道生态失调有关。在此,我们的目的是通过收集HCT后长达1年的纵向粪便样本,对9名儿童异基因HCT参与者进行前瞻性纵向队列研究。粪便微生物群分析表明,异基因HCT和抗生素治疗会导致肠道微生物群多样性的急性变化,与对照组相比,经历3-4期肠道移植物抗宿主病的参与者随着时间的推移,微生物群的变化明显更大(p=0.007)然而,时间并没有达到统计学意义(p=0.05)。未来有必要进行大型前瞻性研究,以阐明儿童异基因HCT后肠道微生物群动态变化的机制。
{"title":"Longitudinal relationship between the gut microbiota variation and diversity and gut graft-versus-host disease (GVHD) following pediatric allogeneic hematopoietic cell transplantation (HCT) – Case series","authors":"Ashley N. Gray ,&nbsp;Nicole H. Tobin ,&nbsp;Theodore B. Moore ,&nbsp;Fan Li ,&nbsp;Grace M. Aldrovandi","doi":"10.1016/j.ijmm.2023.151580","DOIUrl":"10.1016/j.ijmm.2023.151580","url":null,"abstract":"<div><p>Allogeneic Hematopoietic Cell Transplantation (HCT) offers children with life-threatening diseases a chance at survival. Complications from graft-versus-host disease (GVHD, Stages 0–4) represent a significant cause of morbidity and mortality which has been recently associated with gut dysbiosis the adult HCT population. Here, our objective was to conduct a prospective, longitudinal cohort study in nine pediatric allogeneic HCT participants by collecting longitudinally post-HCT stool specimens up to 1 year. Stool microbiota analyses showed that allogeneic HCT and antibiotic therapy lead to acute shifts in the diversity of the gut microbiota with those experiencing stages 3–4 gut GVHD having significantly greater microbiota variation over time when compared to control participants (p = 0.007). Pre-HCT microbiota diversity trended towards an inverse relationship with gut microbiota stability over time, however, this did not reach statistical significance (p = 0.05). Future large prospective studies are necessary to elucidate the mechanisms underlying these dynamic changes in the gut microbiota following pediatric allogeneic HCT.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151580"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9649655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
International Journal of Medical Microbiology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1