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Circulation of Mumps virus genotype G in Pakistan during 2023 outbreak 2023 年疫情爆发期间腮腺炎病毒基因 G 型在巴基斯坦的流行情况
Pub Date : 2024-02-09 DOI: 10.2217/fvl-2023-0145
M. Umair, S. A. Haider, Z. Jamal, R. Hakim, Ammara Farooq, M. Salman
Aim: Despite recurring mumps outbreaks in Pakistan, the virus's genomic diversity remains unavailable. This study explores the mumps genomic diversity in the 2023 Islamabad outbreak. Methods: During January and February 2023, 32 buccal/throat swabs were collected from suspected mumps cases. 15 tested mumps virus (MuV)-positive via RT-PCR. Positive samples underwent partial SH gene sequencing; a subset (n = 10) was whole-genome sequenced using Illumina MiSeq. Results: Phylogenetic analysis identified genotype-G, sharing 95.57–98.73% homology with Japanese/Canadian isolates. Whole-genome-sequencing (n = 2) confirmed genotype-G, 99% similar to Sheffield/Iowa strain. HN region displayed 95.01–95.36% identity with Jeryl Lynn, with genotype G-specific mutations, except T265I. Conclusion: This is the first report of partial and whole-genome sequencing of MuV from Pakistan, highlighting the necessity for genomic surveillance and vaccine integration.
目的:尽管流行性腮腺炎疫情在巴基斯坦反复爆发,但病毒的基因组多样性仍未得到研究。本研究探讨了 2023 年伊斯兰堡疫情中腮腺炎基因组的多样性。研究方法2023 年 1 月和 2 月期间,从疑似流行性腮腺炎病例中采集了 32 份口腔/咽喉拭子。经 RT-PCR 检测,15 例腮腺炎病毒 (MuV) 阳性。阳性样本进行了部分 SH 基因测序;一部分样本(n = 10)使用 Illumina MiSeq 进行了全基因组测序。结果系统发育分析确定了基因型-G,与日本/加拿大分离株有 95.57-98.73% 的同源性。全基因组测序(n = 2)证实了基因型-G,与谢菲尔德/爱荷华菌株的相似度为 99%。HN 区域与 Jeryl Lynn 的同源性为 95.01%-95.36%,除 T265I 外,基因型 G 有特异性突变。结论这是第一份对巴基斯坦 MuV 进行部分和全基因组测序的报告,凸显了基因组监测和疫苗整合的必要性。
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引用次数: 0
Protocol for the Mpox Prospective Observational Cohort Study (MPOCS) among individuals with mpox in Canada 加拿大麻风病人前瞻性观察队列研究(MPOCS)议定书
Pub Date : 2024-02-09 DOI: 10.2217/fvl-2023-0189
Darrell H S Tan, Rob Kozak, Attia Qamar, Reva Persaud, Adrienne K Chan, John Maxwell, Michael Kwag, Allison McGeer, S. Walmsley, Marina B Klein, Mark W Hull, Sharmistha Mishra
In mid-2022, a global mpox epidemic emerged that was concentrated in gay, bisexual and other men who have sex with men, prompting the WHO to declare a public health emergency of international concern. Shortly after cases were first identified in Canada, we established the Mpox Prospective Observational Cohort Study (MPOCS) to characterize clinical, psychosocial, epidemiologic and virologic characteristics of this re-emerging infection. Community members were engaged in identifying research priorities, creating participant-facing materials, and forming a community advisory board. This article outlines the MPOCS study protocol. Although cases of mpox in Canada have rapidly declined, our research procedures may serve as a template for rapidly studying emerging infectious disease threats in the future, particularly among sexual networks.
2022 年年中,全球出现了一种主要集中在男同性恋、双性恋和其他男男性行为者中的麻风腮疫情,促使世界卫生组织宣布进入国际关注的公共卫生紧急状态。在加拿大首次发现病例后不久,我们就建立了痘痘前瞻性观察队列研究(MPOCS),以了解这种重新出现的感染的临床、社会心理、流行病学和病毒学特征。社区成员参与了确定研究重点、制作面向参与者的材料以及组建社区咨询委员会等工作。本文概述了 MPOCS 研究方案。虽然加拿大的麻疹病例已迅速减少,但我们的研究程序可作为未来快速研究新发传染病威胁的模板,尤其是在性网络中。
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引用次数: 0
Title: A computational approach to predict the possible binding site of HCV NS5A and the host cell chaperone, GRP78 标题:预测 HCV NS5A 与宿主细胞伴侣蛋白 GRP78 可能结合位点的计算方法
Pub Date : 2024-02-09 DOI: 10.2217/fvl-2023-0151
Wael M. Elshemey, Abdo A. Elfiky, Alaa M. Elgohary
Aim: Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum located chaperone that plays a vital role during cellular stress. NS5A is one of the hepatitis C virus (HCV) non-structural proteins essential for replication. Materials & methods: Protein–protein docking was used to test the binding mode between GRP78 and HCV NS5A. Molecular dynamics simulations (MDSs) are performed on HCV NS5A, GRP78 and the HCV NS5A–GRP78 complex. Results: Docking and MDS reveal the ability of the GRP78 substrate-binding domain β to associate tightly with the HCV NS5A C142-C165 region. Conclusion: MDS reveals the potential of the C142-C165 region of the HCV NS5A to be used as a seed to develop a recognition inhibitor that counterparts the viral protein recognition by GRP78.
目的:葡萄糖调节蛋白 78(GRP78)是一种位于内质网的伴侣蛋白,在细胞应激过程中发挥着重要作用。NS5A 是丙型肝炎病毒(HCV)复制所必需的非结构蛋白之一。材料与方法:采用蛋白质-蛋白质对接法测试 GRP78 与 HCV NS5A 的结合模式。对 HCV NS5A、GRP78 和 HCV NS5A-GRP78 复合物进行了分子动力学模拟(MDS)。结果:对接和分子动力学模拟揭示了 GRP78 底物结合域 β 与 HCV NS5A C142-C165 区域紧密结合的能力。结论MDS揭示了HCV NS5A的C142-C165区域作为种子选手开发识别抑制剂的潜力,该抑制剂可对抗GRP78对病毒蛋白的识别。
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引用次数: 0
Circulation of Mumps virus genotype G in Pakistan during 2023 outbreak 2023 年疫情爆发期间腮腺炎病毒基因 G 型在巴基斯坦的流行情况
Pub Date : 2024-02-09 DOI: 10.2217/fvl-2023-0145
M. Umair, S. A. Haider, Z. Jamal, R. Hakim, Ammara Farooq, M. Salman
Aim: Despite recurring mumps outbreaks in Pakistan, the virus's genomic diversity remains unavailable. This study explores the mumps genomic diversity in the 2023 Islamabad outbreak. Methods: During January and February 2023, 32 buccal/throat swabs were collected from suspected mumps cases. 15 tested mumps virus (MuV)-positive via RT-PCR. Positive samples underwent partial SH gene sequencing; a subset (n = 10) was whole-genome sequenced using Illumina MiSeq. Results: Phylogenetic analysis identified genotype-G, sharing 95.57–98.73% homology with Japanese/Canadian isolates. Whole-genome-sequencing (n = 2) confirmed genotype-G, 99% similar to Sheffield/Iowa strain. HN region displayed 95.01–95.36% identity with Jeryl Lynn, with genotype G-specific mutations, except T265I. Conclusion: This is the first report of partial and whole-genome sequencing of MuV from Pakistan, highlighting the necessity for genomic surveillance and vaccine integration.
目的:尽管流行性腮腺炎疫情在巴基斯坦反复爆发,但病毒的基因组多样性仍未得到研究。本研究探讨了 2023 年伊斯兰堡疫情中腮腺炎基因组的多样性。研究方法2023 年 1 月和 2 月期间,从疑似流行性腮腺炎病例中采集了 32 份口腔/咽喉拭子。经 RT-PCR 检测,15 例腮腺炎病毒 (MuV) 阳性。阳性样本进行了部分 SH 基因测序;一部分样本(n = 10)使用 Illumina MiSeq 进行了全基因组测序。结果系统发育分析确定了基因型-G,与日本/加拿大分离株有 95.57-98.73% 的同源性。全基因组测序(n = 2)证实了基因型-G,与谢菲尔德/爱荷华菌株的相似度为 99%。HN 区域与 Jeryl Lynn 的同源性为 95.01%-95.36%,除 T265I 外,基因型 G 有特异性突变。结论这是第一份对巴基斯坦 MuV 进行部分和全基因组测序的报告,凸显了基因组监测和疫苗整合的必要性。
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引用次数: 0
Protocol for the Mpox Prospective Observational Cohort Study (MPOCS) among individuals with mpox in Canada 加拿大麻风病人前瞻性观察队列研究(MPOCS)议定书
Pub Date : 2024-02-09 DOI: 10.2217/fvl-2023-0189
Darrell H S Tan, Rob Kozak, Attia Qamar, Reva Persaud, Adrienne K Chan, John Maxwell, Michael Kwag, Allison McGeer, S. Walmsley, Marina B Klein, Mark W Hull, Sharmistha Mishra
In mid-2022, a global mpox epidemic emerged that was concentrated in gay, bisexual and other men who have sex with men, prompting the WHO to declare a public health emergency of international concern. Shortly after cases were first identified in Canada, we established the Mpox Prospective Observational Cohort Study (MPOCS) to characterize clinical, psychosocial, epidemiologic and virologic characteristics of this re-emerging infection. Community members were engaged in identifying research priorities, creating participant-facing materials, and forming a community advisory board. This article outlines the MPOCS study protocol. Although cases of mpox in Canada have rapidly declined, our research procedures may serve as a template for rapidly studying emerging infectious disease threats in the future, particularly among sexual networks.
2022 年年中,全球出现了一种主要集中在男同性恋、双性恋和其他男男性行为者中的麻风腮疫情,促使世界卫生组织宣布进入国际关注的公共卫生紧急状态。在加拿大首次发现病例后不久,我们就建立了痘痘前瞻性观察队列研究(MPOCS),以了解这种重新出现的感染的临床、社会心理、流行病学和病毒学特征。社区成员参与了确定研究重点、制作面向参与者的材料以及组建社区咨询委员会等工作。本文概述了 MPOCS 研究方案。虽然加拿大的麻疹病例已迅速减少,但我们的研究程序可作为未来快速研究新发传染病威胁的模板,尤其是在性网络中。
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引用次数: 0
Title: A computational approach to predict the possible binding site of HCV NS5A and the host cell chaperone, GRP78 标题:预测 HCV NS5A 与宿主细胞伴侣蛋白 GRP78 可能结合位点的计算方法
Pub Date : 2024-02-09 DOI: 10.2217/fvl-2023-0151
Wael M. Elshemey, Abdo A. Elfiky, Alaa M. Elgohary
Aim: Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum located chaperone that plays a vital role during cellular stress. NS5A is one of the hepatitis C virus (HCV) non-structural proteins essential for replication. Materials & methods: Protein–protein docking was used to test the binding mode between GRP78 and HCV NS5A. Molecular dynamics simulations (MDSs) are performed on HCV NS5A, GRP78 and the HCV NS5A–GRP78 complex. Results: Docking and MDS reveal the ability of the GRP78 substrate-binding domain β to associate tightly with the HCV NS5A C142-C165 region. Conclusion: MDS reveals the potential of the C142-C165 region of the HCV NS5A to be used as a seed to develop a recognition inhibitor that counterparts the viral protein recognition by GRP78.
目的:葡萄糖调节蛋白 78(GRP78)是一种位于内质网的伴侣蛋白,在细胞应激过程中发挥着重要作用。NS5A 是丙型肝炎病毒(HCV)复制所必需的非结构蛋白之一。材料与方法:采用蛋白质-蛋白质对接法测试 GRP78 与 HCV NS5A 的结合模式。对 HCV NS5A、GRP78 和 HCV NS5A-GRP78 复合物进行了分子动力学模拟(MDS)。结果:对接和分子动力学模拟揭示了 GRP78 底物结合域 β 与 HCV NS5A C142-C165 区域紧密结合的能力。结论MDS揭示了HCV NS5A的C142-C165区域作为种子选手开发识别抑制剂的潜力,该抑制剂可对抗GRP78对病毒蛋白的识别。
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引用次数: 0
Developing a risk prediction model for COVID-19 infection in heart transplant recipients using artificial intelligence 利用人工智能开发心脏移植受者感染 COVID-19 的风险预测模型
Pub Date : 2024-02-09 DOI: 10.2217/fvl-2023-0162
Shriya Sharma, Nora Menon, Jose Ruiz, Caitlyn Luce, Lisa Brumble, Anirban Bhattacharya, Rohan Goswami
Aim: Describe the utility of an inverse reinforcement learning pathway to develop a novel model to predict and manage the spread of COVID-19. Materials & methods: Convolutional neural network (CNN) with multilayer perceptron (MLP) modeling functions utilized inverse reinforcement learning to predict COVID-19 outcomes based on a comprehensive array of factors. Results: Our model demonstrates a sensitivity of 0.67 in the receiver operating characteristic curve and can correctly identify approximately 67% of the positive cases. Conclusion: We demonstrate the ability to augment clinical decision-making with a novel artificial intelligence (AI) solution that accurately predicted the susceptibility of transplant patients to COVID-19. This enables physicians to administer treatment and take appropriate preventative measures based on patients' risk factors.
目的:描述反强化学习途径在开发新型模型以预测和管理 COVID-19 传播方面的实用性。材料与方法:具有多层感知器(MLP)建模功能的卷积神经网络(CNN)利用反强化学习来预测基于一系列综合因素的 COVID-19 结果。结果我们的模型在接收者操作特征曲线上的灵敏度为 0.67,能正确识别约 67% 的阳性病例。结论我们展示了利用新型人工智能 (AI) 解决方案增强临床决策的能力,该解决方案可准确预测移植患者对 COVID-19 的易感性。这使医生能够根据患者的风险因素进行治疗并采取适当的预防措施。
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引用次数: 0
Preliminary application of a coronavirus pseudovirus system 冠状病毒伪病毒系统的初步应用
Pub Date : 2024-02-05 DOI: 10.2217/fvl-2023-0131
X. Tao, Tengfei Li, Baojun He, Wei Zhao, Siman Hu, Zhuping Ma, Lvyin Sun, Runlin Li, Like Luo, Yonggang Li
Aim: The preparation of a VSVΔG*-S pseudovirus carrying the spike (S) protein of SARS-CoV-2 and its preliminary application. Materials & methods: 293T cells were transfected with pCAGGS-SARS-2-S and infected with VSVΔG viruses. The VSVΔG*-S pseudovirus was collected by centrifugation. The pseudovirus was used to investigate the neutralization ability of serum from SARS-CoV-2 patients and the ability of peptides to inhibit the virus. Results: A VSVΔG*-S pseudovirus was successfully prepared. Patient serum and peptides could neutralize the pseudovirus infection as determined by measuring the expression of GFP. Conclusion: This pseudovirus system can be used to screen polypeptides and fusion inhibitors and measure neutralizing activity, which will benefit the study of SARS-CoV-2.
目的:制备携带SARS-CoV-2尖峰(S)蛋白的VSVΔG*-S假病毒及其初步应用。材料与方法:用 pCAGGS-SARS-2-S 转染 293T 细胞并用 VSVΔG 病毒感染。离心收集 VSVΔG*-S 假病毒。利用假病毒研究 SARS-CoV-2 患者血清的中和能力以及多肽抑制病毒的能力。研究结果成功制备了 VSVΔG*-S 伪病毒。通过测定 GFP 的表达,病人血清和多肽可以中和伪病毒感染。结论该假病毒系统可用于筛选多肽和融合抑制剂,并测量中和活性,这将有利于对 SARS-CoV-2 的研究。
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引用次数: 0
Preliminary application of a coronavirus pseudovirus system 冠状病毒伪病毒系统的初步应用
Pub Date : 2024-02-05 DOI: 10.2217/fvl-2023-0131
X. Tao, Tengfei Li, Baojun He, Wei Zhao, Siman Hu, Zhuping Ma, Lvyin Sun, Runlin Li, Like Luo, Yonggang Li
Aim: The preparation of a VSVΔG*-S pseudovirus carrying the spike (S) protein of SARS-CoV-2 and its preliminary application. Materials & methods: 293T cells were transfected with pCAGGS-SARS-2-S and infected with VSVΔG viruses. The VSVΔG*-S pseudovirus was collected by centrifugation. The pseudovirus was used to investigate the neutralization ability of serum from SARS-CoV-2 patients and the ability of peptides to inhibit the virus. Results: A VSVΔG*-S pseudovirus was successfully prepared. Patient serum and peptides could neutralize the pseudovirus infection as determined by measuring the expression of GFP. Conclusion: This pseudovirus system can be used to screen polypeptides and fusion inhibitors and measure neutralizing activity, which will benefit the study of SARS-CoV-2.
目的:制备携带SARS-CoV-2尖峰(S)蛋白的VSVΔG*-S假病毒及其初步应用。材料与方法:用 pCAGGS-SARS-2-S 转染 293T 细胞并用 VSVΔG 病毒感染。离心收集 VSVΔG*-S 假病毒。利用假病毒研究 SARS-CoV-2 患者血清的中和能力以及多肽抑制病毒的能力。研究结果成功制备了 VSVΔG*-S 伪病毒。通过测定 GFP 的表达,病人血清和多肽可以中和伪病毒感染。结论该假病毒系统可用于筛选多肽和融合抑制剂,并测量中和活性,这将有利于对 SARS-CoV-2 的研究。
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引用次数: 0
Management of viral infections: vaccines or antivirals 病毒感染管理:疫苗或抗病毒药物
Pub Date : 2024-01-25 DOI: 10.2217/fvl-2023-0196
E. D. Clercq
{"title":"Management of viral infections: vaccines or antivirals","authors":"E. D. Clercq","doi":"10.2217/fvl-2023-0196","DOIUrl":"https://doi.org/10.2217/fvl-2023-0196","url":null,"abstract":"","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"14 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139597509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Future Virology
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