Seyed Hamidreza Naghi Mousavi, Alireza Gholami, Mojtaba Jafarinia, Mehdi Ajorloo
Aim: Rabies is one of the most lethal viral diseases. This study developed a recombinant rabies virus, PVEGFP, expressing an EGFP protein based on PV strain to be applied in virus-neutralizing antibody (VNA) titration methods. Methods: The EGFP gene was inserted into the PVMCS strain, a previously discussed recombinant virus. The recombinant virus was applied in the rapid fluorescent focus inhibition test (RFFIT) VNA titration method, and six vaccinated persons were studied for validation. Results: The recombinant rabies strain showed similar propagation properties to its ancestry strain, PVMCS. It could also measure VNA titers in the RFFIT method not significantly different from those acquired using the CVS-11 virus. Conclusion: Utilizing the PVEGFP strain in the RFFIT method can produce rapid, reliable and affordable results.
{"title":"Construction and development of new rabies virus vectors carrying enhanced green fluorescent protein","authors":"Seyed Hamidreza Naghi Mousavi, Alireza Gholami, Mojtaba Jafarinia, Mehdi Ajorloo","doi":"10.2217/fvl-2023-0168","DOIUrl":"https://doi.org/10.2217/fvl-2023-0168","url":null,"abstract":"Aim: Rabies is one of the most lethal viral diseases. This study developed a recombinant rabies virus, PVEGFP, expressing an EGFP protein based on PV strain to be applied in virus-neutralizing antibody (VNA) titration methods. Methods: The EGFP gene was inserted into the PVMCS strain, a previously discussed recombinant virus. The recombinant virus was applied in the rapid fluorescent focus inhibition test (RFFIT) VNA titration method, and six vaccinated persons were studied for validation. Results: The recombinant rabies strain showed similar propagation properties to its ancestry strain, PVMCS. It could also measure VNA titers in the RFFIT method not significantly different from those acquired using the CVS-11 virus. Conclusion: Utilizing the PVEGFP strain in the RFFIT method can produce rapid, reliable and affordable results.","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"18 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139596062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Behrang Sarshari, Raziyeh Zareh-Khoshchehreh, Mehdi Tavallaei, Mohsen Rajabnia, Mohammad Tayefeh Norooz, S. Mohebbi, M. Ravanshad
Aim: To investigate the correlation between Epstein–Barr virus (EBV) variants and EBV-associated gastric cancer (EBVaGC) in Iran. Materials & methods: Biopsies from 21 gastric cancer (4 positive EBER-ISH (EBVaGC) and 17 negative EBER-ISH) and 11 gastritis patients were analyzed using nested PCR and sequencing. Results: Genotype 1 (75%) was the predominant EBV genotype. The P-thr variant was the most common in all groups (positive EBER-ISH 75%, negative EBER-ISH 76.4%, and gastritis 81.8%), followed by P-ala. Additionally, the predominant LMP1 strain in all groups was Med. Sequencing analysis revealed a strong correlation between the P-thr and type 1. Conclusion: Since no preferential correlations were observed between distinct EBV variants and the study groups, these results might indicate geographic polymorphisms.
{"title":"Comparative and phylogenetic analysis of Epstein–Barr virus isolates in gastric cancer and chronic gastritis patients in Iran","authors":"Behrang Sarshari, Raziyeh Zareh-Khoshchehreh, Mehdi Tavallaei, Mohsen Rajabnia, Mohammad Tayefeh Norooz, S. Mohebbi, M. Ravanshad","doi":"10.2217/fvl-2023-0144","DOIUrl":"https://doi.org/10.2217/fvl-2023-0144","url":null,"abstract":"Aim: To investigate the correlation between Epstein–Barr virus (EBV) variants and EBV-associated gastric cancer (EBVaGC) in Iran. Materials & methods: Biopsies from 21 gastric cancer (4 positive EBER-ISH (EBVaGC) and 17 negative EBER-ISH) and 11 gastritis patients were analyzed using nested PCR and sequencing. Results: Genotype 1 (75%) was the predominant EBV genotype. The P-thr variant was the most common in all groups (positive EBER-ISH 75%, negative EBER-ISH 76.4%, and gastritis 81.8%), followed by P-ala. Additionally, the predominant LMP1 strain in all groups was Med. Sequencing analysis revealed a strong correlation between the P-thr and type 1. Conclusion: Since no preferential correlations were observed between distinct EBV variants and the study groups, these results might indicate geographic polymorphisms.","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"88 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139440488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Pouriayevali, Ehsan Mostafavi, M. Fazlalipour, T. Jalali, S. Khakifirouz, Seyed Dawood Mosavi-Nasab, Mostafa Salehi-Vaziri
{"title":"Effect of the COVID-19 pandemic on the reporting of Crimean–Congo hemorrhagic fever","authors":"M. Pouriayevali, Ehsan Mostafavi, M. Fazlalipour, T. Jalali, S. Khakifirouz, Seyed Dawood Mosavi-Nasab, Mostafa Salehi-Vaziri","doi":"10.2217/fvl-2023-0129","DOIUrl":"https://doi.org/10.2217/fvl-2023-0129","url":null,"abstract":"","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"105 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：