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Effect of different LEDs light spectrum on potato (Solanum tuberosum L.) in vitro (review) 不同发光二极管光谱对离体马铃薯(Solanum tuberosum L.)的影响(综述)
Pub Date : 2023-12-15 DOI: 10.30766/2072-9081.2023.24.6.913-92
T. N. Lisina, O. V. Burdysheva, E. S. Sholgin
Potato (Solanum tuberosum L.) is a significant and valuable crop for the economy of many countries. It provides people nutrition and national food security. To obtain healthy potato planting material, propagation in vitro culture is carried out. The problem of increasing the propagation efficiency at this stage is very relevant and can be solved by optimizing the lighting parameters, including the spectral composition of the emitter. The review of published works mainly over the last 20 years concerning the study of the effect of LED lighting of different spectral composition and power on regenerated potato plants, grown in vitro, is given in this paper. Morphometric and physiological parameters of potato plants are given, which can be influenced by changing the spectral composition of illumination. Data on lighting recommendations for different varieties of potato are given. This review may be useful for organizations involved in potato micropropagation, as well as for research teams developing technologies for optimal potato cultivation.
马铃薯(Solanum tuberosum L.)是许多国家的重要经济作物。它为人们提供营养和国家粮食安全。为了获得健康的马铃薯种植材料,需要进行离体培养繁殖。在这一阶段,提高繁殖效率的问题非常重要,可以通过优化照明参数(包括发射器的光谱成分)来解决。本文回顾了主要是过去 20 年发表的关于不同光谱成分和功率的 LED 照明对离体生长的再生马铃薯植株的影响的研究成果。文中给出了马铃薯植株的形态和生理参数,这些参数可通过改变光照的光谱成分产生影响。文中还给出了针对不同马铃薯品种的光照建议数据。这篇综述可能对从事马铃薯微繁殖的组织以及开发最佳马铃薯栽培技术的研究小组有用。
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引用次数: 0
The influence of the duration of the storage period of aeroponic minitubers on the growth and development of potato varieties in field conditions 气培微型管的储存期对马铃薯品种在田间条件下生长发育的影响
Pub Date : 2023-12-15 DOI: 10.30766/2072-9081.2023.24.6.969-979
K. A. Koloshina, N. I. Polukhin, K. V. Avdeenko
When using minitubers as planting material in seed production, there is a problem of sparseness of seedlings, which is associated with different duration of the storage period of minitubers (since harvesting from an aeroponic installation to the appearance of the first single shoots). The purpose of the research is to establish the influence of the duration of the storage period of aeroponic minitubers on the growth, development and productivity of various potato varieties under field conditions. The research was carried out in 2020-2022 in the conditions of the Novosibirsk region, the soil is leached medium loamy chernozem. The objects of the research are aeroponic minitubers of early-ripening (Yuna, Terra, Legenda) and mid-ripening (Zlatka, Sokur) potato varieties for December, March and April harvesting periods. It was established that the storage period of minitubers of various varieties was 179-184 days when collected from an aeroponic installation in December, 91.7-118.3 days in March, 71.3-99.7 days in April. The use of minitubers as planting material for growing high-quality potato seeds is justified during the December and March harvesting periods. Potato plants from the December planting material had a germination rate of 98.5 %, germinated 20 days earlier, had a larger average leaf area, the initial yield on the first recording date was 2.6 times greater and the final yield was 1.4 times greater than the plants from the March planting material. Minitubers of the December period provided the highest yield (22.8 t/ha) and the yield of seed tubers (76.3 %), with March harvest time the corresponding values were lower by 6.8 t/ha and 15.9 %, with April harvest period – by 15.9 t/ha and 34,0 %. It is not practical to use minitubers of the April harvest time with a storage period of 91.7-118.3 days as planting material, since it is impossible to obtain yield for the most of the studied varieties. The Sokur (mid-ripening) and Terra (early-ripening) varieties showed the highest yields of 20.4 and 19.5 t/ha.
在种子生产中使用微型管作为种植材料时,会出现幼苗稀疏的问题,这与微型管的储存期(从从气培装置收获到出现第一个单芽)长短不同有关。研究的目的是确定在田间条件下,气培微型管的储存期对各种马铃薯品种的生长、发育和产量的影响。该研究于 2020-2022 年在新西伯利亚地区的土壤条件下进行,土壤为沥滤中壤土 Chernozem。研究对象是 12 月、3 月和 4 月收获期的早熟(尤娜、特拉、列根达)和中熟(兹拉特卡、索库尔)马铃薯品种的气培微型管。结果表明,12 月从气培装置中采集的不同品种迷你管的储存期为 179-184 天,3 月为 91.7-118.3 天,4 月为 71.3-99.7 天。在 12 月和 3 月的收获期,使用微型管作为种植材料来培育优质马铃薯种子是合理的。12 月种植材料的马铃薯植株发芽率为 98.5%,发芽时间提前 20 天,平均叶面积更大,第一个记录日期的初始产量是 3 月种植材料植株的 2.6 倍,最终产量是其 1.4 倍。12 月份收获的微型块茎产量最高(22.8 吨/公顷),块茎种子产量最高(76.3%),3 月份收获的微型块茎产量和种子产量分别低 6.8 吨/公顷和 15.9%,4 月份收获的微型块茎产量和种子产量分别低 15.9 吨/公顷和 34.0%。使用 4 月收获期、储存期为 91.7-118.3 天的迷你管作为种植材料是不切实际的,因为大多数研究品种都无法获得产量。Sokur(中熟)和 Terra(早熟)品种的产量最高,分别为 20.4 吨/公顷和 19.5 吨/公顷。
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引用次数: 0
Clearance tests as a diagnosis method of hepatobiliary system pathologies in animals 作为动物肝胆系统疾病诊断方法的清除试验
Pub Date : 2023-12-15 DOI: 10.30766/2072-9081.2023.24.6.924-938
V. S. Ponamarev, O. Popova, A. Kostrova, L. Agafonova
In present-day conditions of industrial agriculture, the animal's body works at the limit of its capabilities, which leads to degenerative diseases of various organs and systems. The development of ergonomic ways of modern diagnosis of these pathologies at the early stages contributes to the maintenance of quantitative and qualitative indices of productivity of animals and to the extension of the time of their economic use. The liver with a great number of functions performed is subjected to the highest load. The aim of the article is to review the available clearance tests in order to diagnose the pathology of the hepatobiliary system of animals at the early stage, considering advantages and disadvantages of the tests. As the result of the search of themed issues in various bibliographic databases there have been selected 45 mostly cited scientific articles. The article reviews such clearance tests as indocyanine green clearance test, aminopyrine breath test, sorbitol elimination test, galactose elimination test, phenylalanine breath test, and hepatobiliary scintigraphy method. Functional tests allow not only diagnosing and differentiating hepatopathologies, but also visualizing their course and liver recovery. Unlike laboratory tests, clearance tests make it possible to evaluate the work of the liver in dynamics. The disadvantages of clearance tests are lack of information for their wide use and frequency of particular differences in elimination of specific substances. To reduce the proportion of existing shortcomings, it is possible to combine clearance tests with other laboratory methods for diagnosing hepatopathy in animals.
在当今的工业化农业条件下,动物身体的工作能力已达到极限,这导致了各种器官和系统的退化性疾病。开发符合人体工程学的方法,在早期阶段对这些病症进行现代诊断,有助于保持动物生产的数量和质量指标,延长动物的经济利用时间。肝脏承担着大量的功能,承受着最大的负荷。本文旨在回顾现有的清除试验,以便在早期阶段诊断动物肝胆系统的病变,同时考虑到这些试验的优缺点。通过在各种文献数据库中对主题问题的搜索,选出了 45 篇引用率最高的科学文章。文章回顾了吲哚菁绿清除试验、氨基嘌呤呼气试验、山梨醇消除试验、半乳糖消除试验、苯丙氨酸呼气试验和肝胆闪烁法等清除试验。功能检查不仅可以诊断和区分肝脏病变,还可以直观地了解病变过程和肝脏恢复情况。与实验室检测不同,清除率检测可以动态评估肝脏的工作情况。清除率检测的缺点是缺乏广泛使用的信息,以及在消除特定物质时出现特殊差异的频率。为了减少现有缺点的比例,可以将清除试验与其他实验室方法结合起来诊断动物肝病。
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引用次数: 0
A new variety of spring barley Boyarin and elements of varietal technology 春大麦新品种 Boyarin 和品种技术要素
Pub Date : 2023-12-15 DOI: 10.30766/2072-9081.2023.24.6.939-948
I. Shchennikova, T. Sheshegova, O. Snigireva
The development of the Boyarin spring barley variety was carried out in 2008-2022, the improvement of varietal agricultural technology – in 2021-2022 in the soil and climatic conditions of the Kirov region. The Boyarin spring barley variety is characterized by a high yield (up to 6.15 t/ha in 2017), in some years exceeding the Belgorod 100 standard (by 0.9 t/ha in 2018). The variety is medium-early, the duration of the growing season is from 76 to 97 days; forms a highly productive stem (390 pcs/m2) due to good survival and bushiness of plants (2.0 pcs/plant), has a strong straw and is characterized by high resistance to lodging (5.0 points). The weight of 1000 grains is up to 47 g, the nature is up to 683 g/l, the protein content in the grain is up to 12.2 %. According to the quality of grain, it belongs to the varieties of grain-forage use. Since 2023, the Boyarin variety has been included in the State Register of Breeding Achievements Approved for Use in the Volga-Vyatka region. The article provides a scheme for creating a new variety of spring barley Boyarin and its economic and biological characteristics. When developing elements of varietal technology, new biofertilizers (Azolene, Azotovite, Phosphatovite, Kalivite, Nitroslak) andbiofungicides (Pseudobacterin, Flavobacterin, Alirin) were tested, which were used to treat seeds and plants in the "tillering" and "earing" phases. In relatively arid vegetation conditions (HTC = 1.23), no statistically significant yield increases were obtained from the use of all the studied preparations. With excessive moisture during the growing season of plants (HTC = 2.20) relatively high yield increases (1.31-1.58 t/ha with LSD05 = 0.50) were obtained in variants using biofertilizers Phosphatovite (seed treatment), Azotovite (plant treatment in the "earing" phase) and Kaliivit (treatment of plants in the "tillering" phase). The best biological protection against root rot at the level of 60.5 % was provided by the treatment of seeds with Nitroslak biofertilizer, which is not only a nitrogen fixator and phosphate immobilizer, but it also contains a binary mixture of microorganisms antagonists of phytopathogens.
根据基洛夫地区的土壤和气候条件,博亚林春大麦品种的开发工作于 2008-2022 年进行,品种农业技术的改进工作于 2021-2022 年进行。博亚林春大麦品种的特点是产量高(2017 年达到 6.15 吨/公顷),有些年份超过了别尔哥罗德 100 标准(2018 年超过 0.9 吨/公顷)。该品种属中早熟品种,生长期为 76 至 97 天;由于植株成活率和灌浆率高(2.0 株/株),茎秆产量高(390 株/平方米),秸秆粗壮,抗倒伏能力强(5.0 分)。千粒重高达 47 克,性质高达 683 克/升,谷物中蛋白质含量高达 12.2%。根据谷物品质,该品种属于粮饲兼用型品种。自 2023 年起,"博亚林 "品种被列入《伏尔加-维亚特卡地区批准使用的育种成果国家登记册》。文章介绍了春大麦新品种博亚林的培育计划及其经济和生物学特性。在开发品种技术要素时,对新型生物肥料(Azolene、Azotovite、Phosphatovite、Kalivite、Nitroslak)和生物杀菌剂(Pseudobacterin、Flavobacterin、Aligin)进行了试验,在 "分蘖期 "和 "抽穗期 "对种子和植物进行处理。在相对干旱的植被条件下(HTC = 1.23),使用所有研究的制剂在统计学上都没有显著的增产效果。在植物生长季节水分过多的情况下(HTC = 2.20),使用磷灰石(种子处理)、Azotovite("抽穗 "阶段的植物处理)和 Kaliivit("分蘖 "阶段的植物处理)生物肥料的变体可获得相对较高的增产(1.31-1.58 吨/公顷,LSD05 = 0.50)。使用 Nitroslak 生物肥料处理种子,对根腐病的生物防治效果最好,达到 60.5%,这种生物肥料不仅是固氮剂和磷酸盐固定剂,还含有拮抗植物病原体的微生物二元混合物。
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引用次数: 0
Resistance of flax gene pool samples to edaphic stress caused by low acidity 亚麻基因库样本对低酸度造成的土壤胁迫的抗性
Pub Date : 2020-04-22 DOI: 10.30766/2072-9081.2020.21.2.133-140
T. Rozhmina, A. A. Zhuchenko Jr., N. Melnikova, A. D. Smirnova
In conditions of vegetative trial carried out against selective backgrounds in 2017-2019 the response of 27 flax samples to a decrease in soil acidity to neutral pHKCl was studied. The scheme of the experiment was as follows: variant I (control) − pHKCl 5.3-5.5, P2O5 − 320-340 mg/kg, K2O − 81-92 mg/kg; variant II − pHKCl 6.2, P2O5 − 312-345 mg/kg, K2O − 84-98 mg/kg. It has been shown that during the «herringbone» phase in the majority of studied flax genotypes against the background of pH 6.2, the symptoms of “physiological oppression” of flax were observed: small spots developed on the upper leaves, the plants stopped growing, the stems thickened, and the tops of severely affected plants died off. As a result, at the beginning of the growing season at plant height of 7-10 cm, most of the samples were severely affected (from 69 to 100 %). The exceptions were varieties of fibre-flax Hermes (France), Vega 2 (Lithuania), Atlant (Russia) and linseed genotypes No. 3896 (Russia) and Norlin (Canada), which had a weak and medium degree of affection (8.3-45.5 %). Moreover, these genotypes showed a high level of both biological (75-90 %) and agronomic (77.3-85.6 %) resistance in the phase of "early yellow ripeness". The identified flax collection samples can be used as sources of resistance to flax «physiological oppression» caused by stressful edaphic factors in a neutral environment. On the basis of the analysis of the main elements of fiber productivity in studied flax genotypes, it has been established that against the background of pH 6.2 the reduce in plant height was from 11.4 to 52.1 % relative to the control, weight of the technical part of the stem − from 7.2 to 83.4 % , fiber mass − from 9.6 to 85.1 %. For the first time, on the basis of hybridological analysis, an assumption was done as to the pres-ence of a strong dominant gene, controlling the resistance to high soil pH values in the Hermes (France) flax variety and the linseed line No. 3896 (Russia).
2017-2019年,在选择性背景下进行的无性系试验条件下,研究了27个亚麻样本对土壤酸度下降至中性pHKCl的反应。实验方案如下:变体 I(对照)--pHKCl 5.3-5.5,P2O5 - 320-340 mg/kg,K2O - 81-92 mg/kg;变体 II - pHKCl 6.2,P2O5 - 312-345 mg/kg,K2O - 84-98 mg/kg。研究表明,在 pH 值为 6.2 的背景下,大多数亚麻基因型在 "人字形 "阶段都会出现亚麻的 "生理抑制 "症状:上部叶片出现小斑点,植株停止生长,茎变粗,严重受害植株的顶端枯死。因此,在生长季节初期,当植株高度为 7-10 厘米时,大多数样本都受到严重影响(从 69% 到 100%)。例外的是纤维亚麻品种 Hermes(法国)、Vega 2(立陶宛)、Atlant(俄罗斯)以及亚麻籽基因型 3896 号(俄罗斯)和 Norlin(加拿大),它们的受害程度为轻度和中度(8.3%-45.5%)。此外,这些基因型在 "早黄成熟 "阶段表现出较高的生物抗性(75-90 %)和农艺抗性(77.3-85.6 %)。经鉴定的亚麻采集样本可用作中性环境下亚麻对胁迫性环境因素造成的 "生理压迫 "的抗性来源。根据对所研究亚麻基因型纤维生产率主要因素的分析,确定在 pH 值为 6.2 的背景下,植株高度比对照降低了 11.4% 至 52.1%,茎的技术部分重量降低了 7.2% 至 83.4%,纤维质量降低了 9.6% 至 85.1%。在杂交分析的基础上,首次推测在赫尔墨斯(法国)亚麻品种和第 3896 号亚麻籽品系(俄罗斯)中存在一个强显性基因,控制着对高土壤 pH 值的抗性。
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引用次数: 3
Resistance of flax gene pool samples to edaphic stress caused by low acidity 亚麻基因库样本对低酸度造成的土壤胁迫的抗性
Pub Date : 2020-04-22 DOI: 10.30766/2072-9081.2020.21.2.133-140
T. Rozhmina, A. A. Zhuchenko Jr., N. Melnikova, A. D. Smirnova
In conditions of vegetative trial carried out against selective backgrounds in 2017-2019 the response of 27 flax samples to a decrease in soil acidity to neutral pHKCl was studied. The scheme of the experiment was as follows: variant I (control) − pHKCl 5.3-5.5, P2O5 − 320-340 mg/kg, K2O − 81-92 mg/kg; variant II − pHKCl 6.2, P2O5 − 312-345 mg/kg, K2O − 84-98 mg/kg. It has been shown that during the «herringbone» phase in the majority of studied flax genotypes against the background of pH 6.2, the symptoms of “physiological oppression” of flax were observed: small spots developed on the upper leaves, the plants stopped growing, the stems thickened, and the tops of severely affected plants died off. As a result, at the beginning of the growing season at plant height of 7-10 cm, most of the samples were severely affected (from 69 to 100 %). The exceptions were varieties of fibre-flax Hermes (France), Vega 2 (Lithuania), Atlant (Russia) and linseed genotypes No. 3896 (Russia) and Norlin (Canada), which had a weak and medium degree of affection (8.3-45.5 %). Moreover, these genotypes showed a high level of both biological (75-90 %) and agronomic (77.3-85.6 %) resistance in the phase of "early yellow ripeness". The identified flax collection samples can be used as sources of resistance to flax «physiological oppression» caused by stressful edaphic factors in a neutral environment. On the basis of the analysis of the main elements of fiber productivity in studied flax genotypes, it has been established that against the background of pH 6.2 the reduce in plant height was from 11.4 to 52.1 % relative to the control, weight of the technical part of the stem − from 7.2 to 83.4 % , fiber mass − from 9.6 to 85.1 %. For the first time, on the basis of hybridological analysis, an assumption was done as to the pres-ence of a strong dominant gene, controlling the resistance to high soil pH values in the Hermes (France) flax variety and the linseed line No. 3896 (Russia).
2017-2019年,在选择性背景下进行的无性系试验条件下,研究了27个亚麻样本对土壤酸度下降至中性pHKCl的反应。实验方案如下:变体 I(对照)--pHKCl 5.3-5.5,P2O5 - 320-340 mg/kg,K2O - 81-92 mg/kg;变体 II - pHKCl 6.2,P2O5 - 312-345 mg/kg,K2O - 84-98 mg/kg。研究表明,在 pH 值为 6.2 的背景下,大多数亚麻基因型在 "人字形 "阶段都会出现亚麻的 "生理抑制 "症状:上部叶片出现小斑点,植株停止生长,茎变粗,严重受害植株的顶端枯死。因此,在生长季节初期,当植株高度为 7-10 厘米时,大多数样本都受到严重影响(从 69% 到 100%)。例外的是纤维亚麻品种 Hermes(法国)、Vega 2(立陶宛)、Atlant(俄罗斯)以及亚麻籽基因型 3896 号(俄罗斯)和 Norlin(加拿大),它们的受害程度为轻度和中度(8.3%-45.5%)。此外,这些基因型在 "早黄成熟 "阶段表现出较高的生物抗性(75-90 %)和农艺抗性(77.3-85.6 %)。已鉴定的亚麻采集样本可用作中性环境下亚麻对胁迫性环境因素造成的 "生理压迫 "的抗性来源。根据对所研究亚麻基因型纤维生产率主要因素的分析,确定在 pH 值为 6.2 的背景下,植株高度比对照降低了 11.4% 至 52.1%,茎的技术部分重量降低了 7.2% 至 83.4%,纤维质量降低了 9.6% 至 85.1%。在杂交分析的基础上,首次推测在赫尔墨斯(法国)亚麻品种和第 3896 号亚麻籽品系(俄罗斯)中存在一个强显性基因,控制着对高土壤 pH 值的抗性。
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引用次数: 3
期刊
Agricultural Science Euro-North-East
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