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Weed infestation of narrow-leaved lupine crops in grain-grass-rowed crop rotation in the North-West of Russia 俄罗斯西北部谷草轮作中窄叶羽扇豆作物的杂草虫害
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.43-52
A. Shpanev
The results of the long term (2012–2018) study of weed infestation of green fallow fields with narrow-leaved lupin in the crop rotation including cereals, grasses and row crops in the North-West region of Russia (Leningrad region) are presented. The assessment of weediness in the narrow-leaved lupine variety Oligarch crops was carried out annually at 24 permanent survey sites (0.1 square meter). Based on the results of the surveys, the formation of a diverse and constant species composition of weeds (from 15 to 25 species), mainly of medium and severe degree of weediness (141–593 specimens per square meter), with the proportion of young plants ranging from 90.4 to 99.7 %. The mass species of weeds were lamb’s quarters, field pansy, corn spurry, hemp-nettles, fumitory, shepherd’s purse, prevalent perennial weeds were field sowthistle, coltsfoot, red sorrel, couch grass, marsh woundwort. The prevailing influence of weather conditions and individual characteristics on the weed infestation of narrow-leaved lupine crop has been determined. The combined effect of two factors was expressed by 47.7, 57.8 and 47.1 % in terms of species abundance, initial abundance and final phytomass of weeds, respectively. Long term application of mineral fertilizers led to an increase in the number of annuals (by 1.5–1.6 times) and a decrease in the density of growth of perennial weed species (by 1.6–1.9 times). The influence of the integrated plant protection system used in the crop rotation was manifested in a significant decrease in the density of perennial weed species (by 4.6 times) in narrow-leaved lupine crop.
本文介绍了对俄罗斯西北部地区(列宁格勒州)轮作谷物、禾本科植物和连作作物中种植狭叶羽扇豆的绿色休耕地杂草侵扰情况进行长期(2012-2018 年)研究的结果。每年在 24 个永久性调查点(0.1 平方米)对窄叶羽扇豆品种 Oligarch 作物的杂草情况进行评估。根据调查结果,杂草的种类组成多样且稳定(15 至 25 种),主要为中度和重度杂草(每平方米 141 至 593 个标本),幼苗所占比例为 90.4% 至 99.7%。主要杂草种类有羊角芹、田堇、玉米穗、麻荨麻、熏草、牧草,多年生杂草主要有田耧斗菜、马蹄金、红苏子、榻榻米、沼泽伤寒草。确定了天气条件和个体特征对窄叶羽扇豆作物杂草侵染的主要影响。从杂草的种类丰度、初始丰度和最终植物量来看,两个因素的综合影响分别为 47.7%、57.8% 和 47.1%。长期施用矿物肥料导致一年生杂草数量增加(1.5-1.6 倍),多年生杂草生长密度降低(1.6-1.9 倍)。轮作中使用的综合植物保护系统的影响表现在窄叶羽扇豆作物中多年生杂草的密度显著下降(4.6 倍)。
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引用次数: 0
Research results of the screw dispenser of dry bulk feed components 干散装饲料成分螺旋分配器的研究成果
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.123-133
S. Bulatov, A. N. Pronin
In the production of compound feeds, it is necessary to follow the recipe at the time of introducing all the necessary components, for which various types of dispensers are used. Currently, automatic dosing systems are widely used, providing increased dosing accuracy. However, individual settings of such systems are required for each component, which makes the work of operators harder. The article describes the design of a technical device containing a main and an additional screw and allowing components with different physical and mechanical properties to be weighed at the same tuning parameters. A brief description of the dispenser's workflow is given, the research factors and criteria for evaluating its work are listed. The influence of five factors was determined: the frequency setpoint of the dispensers n1, the pre-filling setpoint m, the setpoint for switching on the filling speed t, the frequency setpoint of the dispensers n2, the set weight of the suspension MZ on the dosing accuracy. At the first stage, the influence of the studied factors on grain dosing was determined. To do this, the Box-Benkin plan was implemented for five factors. As a result of the analysis of the data obtained, a regression model was built, on the basis of which three combinations of factor values were determined, in which the grain dosing error by an additional dispenser screw was 0. At the next stage of the research, the accuracy of dosing chalk and salt was determined at the established combinations. It was revealed that salt dosing was carried out according to a pattern close to the grain curve, and the dosing error was within the permissible limits of 5%. As a result of the conducted studies, the best combination of factors was determined, in which the dosing error did not exceed the permissible value when weighing grain, salt and chalk: n1 = 30.3 Hz, t = 11.9 %; n2 = 40.9 % of n1 (12.42 Hz), MZ = 202.8 g. With these values of the studied parameters, it is possible to dose components with the permissible error of the weight of the suspension is over 200 g.
在生产配合饲料时,必须按照配方引入所有必要成分,为此需要使用各种类型的配料机。目前,自动配料系统得到广泛应用,提高了配料的准确性。然而,这种系统需要对每种成分进行单独设置,这就增加了操作员的工作难度。文章介绍了一种技术装置的设计,该装置包含一个主螺杆和一个附加螺杆,可在相同的调整参数下称量具有不同物理和机械特性的成分。文章简要介绍了分配器的工作流程,并列出了评估其工作的研究因素和标准。确定了五个因素对配料精度的影响:配料机频率设定值 n1、预填充设定值 m、填充速度开关设定值 t、配料机频率设定值 n2、悬浮液设定重量 MZ。在第一阶段,确定了所研究因素对谷物配料的影响。为此,对五个因素实施了方框-本金计划。对所获得的数据进行分析后,建立了一个回归模型,并在此基础上确定了三个因素值组合,在这三个组合中,额外分配器螺杆的谷物配料误差为 0。结果表明,盐的配料是按照接近谷物曲线的模式进行的,配料误差在 5%的允许范围内。经过研究,确定了最佳的因素组合,在称量谷物、盐和白垩时,配料误差不超过允许值:n1 = 30.3 Hz,t = 11.9 %;n2 = n1 的 40.9 %(12.42 Hz),MZ = 202.8 g。
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引用次数: 0
Dynamics of humus in field crop rotations on drained lands 排水地田间作物轮作中腐殖质的动态变化
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.62-74
Yury I. Mitrofanov, N. Pervushina
The purpose of the research is to study the influence of different types of field crop rotation, fertilizers, drainage and hydrothermal conditions on the dynamics of humus in reclaimed soil. The studies were carried out on the experimental fields of the All-Russian Research Institute of Reclaimed Lands (reclamation sites “Kuzminskoye Boloto 2”, “Semenovskoye” and “Gubino” in the Tver region). Waterlogged soils were drained using closed drainage (inter-drain distance is 18–20 m, drain depth is 0.9–1.2 m). The soils of the experimental plots are soddy-podzolic, light loamy, gleyic, formed on a moraine or thin binomial. Observations of the dynamics of humus were carried out in the grain-grass-row, grain-grass, grain and grain-row types of crop rotation. The influence of crop rotation on the content of humus in the soil was determined, first of all, by the composition, the structure of the crops grown and agricultural technology of their cultivation. Due to plant residues, 56.6–76.5 % of the volume of mineralized humus were restored in grain-grass-row crop rotation, 51.8 % in grain, 26.4 % in grain-row crop rotation. The most significant qualitative changes in the composition of humus were observed in grain-grass-row crop rotation: with a positive humus balance, the ratio of humic and fulvic acids increased from 0.63 to 0.74. The use of organic and mineral fertilizers significantly changes the balance of humus in the arable layer of the soil. With an organic-mineral fertilizer system, the annual loss of humus (in kg/ha) on the drained plot was 6.8–11.4 times less, on the non-drained plot – 2.1–2.6 times less than in the variant without fertilizers. Drainage of waterlogged soils increases the role of fertilizers in the accumulation of humus, reduces its losses and improves the quality parameters of humus – the ratio of humic and fulvic acids in the composition of humus under the influence of drainage increased from 0.61 to 0.88. The impact on the dynamics of humus of hydrothermal conditions has been established. In years with a dry first half of the growing season, an increase in humus content is observed; in excessively wet years, on the contrary, a decrease is observed. The correlation coefficients of humus content with hydrothermal conditions in May-June were – 0.84 (in 1985–1993) and 0.95 (in 2014–2022).
研究的目的是研究不同类型的田间轮作、肥料、排水和水热条件对开垦土壤中腐殖质动态的影响。研究在全俄开垦土地研究所的试验田(特维尔地区的 "库兹明斯科伊博洛托 2 号"、"谢苗诺夫斯科伊 "和 "古比诺 "开垦地)上进行。积水土壤采用封闭排水法(排水间距为 18-20 米,排水深度为 0.9-1.2 米)。实验地块的土壤是在冰碛或薄二项式冰碛上形成的草皮-膏状、轻壤土、格列土。腐殖质的动态观测是在谷-草-行、谷-草、谷物和谷-行轮作类型中进行的。轮作对土壤中腐殖质含量的影响首先取决于种植作物的成分、结构和农业种植技术。由于植物残留物的存在,在谷物-草地-行轮作中,矿化腐殖质的体积恢复了 56.6-76.5%,在谷物-行轮作中恢复了 51.8%,在谷物-行轮作中恢复了 26.4%。在谷物-禾本科作物轮作中,腐殖质成分发生了最明显的质变:在腐殖质正平衡的情况下,腐殖酸和富勒酸的比例从 0.63 增加到 0.74。有机肥和矿物肥的使用极大地改变了土壤耕作层的腐殖质平衡。使用有机-矿物肥料系统后,排水地块的腐殖质年损失量(公斤/公顷)是不施肥地块的 6.8-11.4 倍,是不排水地块的 2.1-2.6 倍。排涝增加了肥料在腐殖质积累中的作用,减少了腐殖质的损失,并改善了腐殖质的质量参数--在排涝的影响下,腐殖质成分中腐殖酸和富勒酸的比例从 0.61 增加到 0.88。水热条件对腐殖质动态的影响已经得到证实。在生长季节前半段干燥的年份,腐殖质含量会增加;相反,在过度潮湿的年份,腐殖质含量会减少。腐殖质含量与 5-6 月热液条件的相关系数分别为-0.84(1985-1993 年)和 0.95(2014-2022 年)。
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引用次数: 0
Relationship between evaluations of sires in pale-motley cattle population at different levels of management 不同管理水平下苍白莫特利牛种群母牛评价之间的关系
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.82-89
N. S. Altukhova, I. Yanchukov, A. V. Savinov, Yu. A. Ivanov
Reproductive technologies have led to a wide and global distribution of genetic material from animals with high productivity. However, the distribution of semen from the same bulls to different regions (countries) may not always meet the expectations of livestock breeders. This aspect may be due to the fact that the genetic structure of the breeding stock, breeding goals and environmental conditions vary from region to region. The article presents the results of evaluating the breeding value of 286 sires used on the breeding stock of the pale-motley dairy cattle population in five regions of the Russian Federation (Belgorod, Voronezh, Kursk, Oryol regions and Altai Territory). The evaluation was carried out according to individual traits of daughters' milk productivity, and on multiple traits (selection index). Based on the results of the study, differences were identified in estimates of the breeding value of the same sires at the level of total information (population level) and in the herds of single regions (regional level). The accuracy of evaluating genotypes at the population management level was significantly higher (by 7–15 percent) than it was at regional levels. The correlations between the breeding value of the same sires at different levels of management (population-region) for single traits of daughter’s milk productivity were 0.522–0.960, for the complex of traits (selection index) – from 0.157 to 0.937. This indicates that when selecting the best sires at the level of an individual region, mistakes can reach 4–48 % for single milk productivity traits and from 6.3 to 84 % for their complex.
繁殖技术使高产动物的遗传物质得以在全球范围内广泛传播。然而,同一头公牛的精液在不同地区(国家)的分配并不总能满足牲畜育种者的期望。这可能是由于不同地区的种畜遗传结构、育种目标和环境条件各不相同。文章介绍了对俄罗斯联邦五个地区(别尔哥罗德州、沃罗涅日州、库尔斯克州、奥廖尔州和阿尔泰边疆区)白蹄奶牛种群中使用的 286 种父本的育种价值进行评估的结果。评估根据女儿产奶量的单项性状和多项性状(选择指数)进行。研究结果表明,在总体信息水平(群体水平)和单个地区(地区水平)的牛群中,相同父本的育种价值估计值存在差异。种群管理水平上的基因型评估准确率(7%-15%)明显高于地区水平。在不同管理水平(种群-地区)下,相同父本的育种价值在女儿奶产量单一性状上的相关系数为 0.522-0.960,在复合性状(选择指数)上的相关系数为 0.157-0.937。这表明,在单个地区水平上选择最佳父本时,单一奶产量性状的错误率可达 4-48%,复合性状的错误率可达 6.3-84%。
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引用次数: 0
Mathematical modeling of dynamic processes of agricultural mobile energy vehicles on an electric drive 电驱动农业移动能源车辆动态过程的数学建模
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.112-122
Z. A. Godzhaev, S. Senkevich, I. S. Alekseev, E. N. Ilchenko
The article considers the issues of modeling the processes of agricultural mobile energy vehicles (MEV) with electric drive (ED). A review of modern literature on the problem under study as well as questions on modeling the functional properties of mobile machines and improving the quality indicators of MEV work are presented. A mathematical model of the motion of the MEV with an electric motor is presented, as well as a description of the method used and a sequence of actions for conducting research. Preliminary theoretical studies of motion in different modes of operation have been carried out. The proposed model is convenient for implementation and calculation in any of the applied software products that support the modeling of dynamic systems with an electromechanical drive. The proposed model, the solution of which is based on the methods of numerical integration of systems in the Matlab Simulink software environment, made it possible to simulate the dynamic processes of electromechanical power transmission of MEV during various agricultural operations. With the help of this model, the analysis of electromechanical processes in transient and steady-state operating modes, as well as dynamic processes in the power transmission were carried out. Graphs of changes in the studied parameters of the MEV power transmission are obtained and elastic moments in the joints of the 5-mass design scheme are determined. The use of the model allows you to track the change in statistical characteristics when the conditions of the experiment change. The model has shown its operability when performing simulation of agricultural operations: fertilization, cultivation and sowing, and it can be used at the design stage to study the characteristics of dynamic processes of small-class traction MEV power transmissions with an electromechanical drive. With different parameters of the model, there is a change in the mathematical expectation of the angular velocity of the ED from 147.89 to 156.87 rad/s, a change in the mathematical expectation of the speed of the MES from 4.51 to 4.79 m/s.
文章探讨了电驱动农用移动能源车(MEV)的过程建模问题。文章回顾了有关所研究问题的现代文献,以及有关移动机械功能特性建模和提高 MEV 工作质量指标的问题。介绍了带电动机的 MEV 运动数学模型,并说明了开展研究的方法和行动顺序。对不同运行模式下的运动进行了初步理论研究。提出的模型便于在任何支持机电驱动动态系统建模的应用软件产品中实施和计算。所提出的模型是基于 Matlab Simulink 软件环境中的系统数值积分方法求解的,它使模拟各种农业作业过程中 MEV 机电传动的动态过程成为可能。在该模型的帮助下,对瞬态和稳态运行模式下的机电过程以及动力传输的动态过程进行了分析。获得了所研究的 MEV 动力传输参数的变化图,并确定了 5 质量设计方案中关节的弹性力矩。使用该模型可以跟踪实验条件发生变化时统计特征的变化。该模型在模拟农业作业(施肥、耕作和播种)时显示了其可操作性,并可在设计阶段用于研究带有机电驱动装置的小型牵引式 MEV 动力传动装置的动态过程特征。在模型参数不同的情况下,ED 角速度的数学期望值从 147.89 拉德/秒变为 156.87 拉德/秒,MES 速度的数学期望值从 4.51 米/秒变为 4.79 米/秒。
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引用次数: 0
Justification of innovative technology for variety and phytocleaning in breeding and seed plantings of potatoes and vegetable crops 在马铃薯和蔬菜作物的育种和种子种植中采用品种和植物清洁创新技术的理由
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.98-111
A. Dorokhov, A. Aksenov, A. Sibirev, M. Mosyakov, N. Sazonov
The purpose of the research is to substantiate theoretically the process of variety and phytocleaning in breeding and seed plantings of potatoes and vegetable crops using machine vision technologies and robotic elements. The article analyzes modern non-destructive methods for detecting diseases of biological objects; technological processes and machines for removing the fruits of vegetable crops from plants in a digital agricultural production system with elements of robotization in the operations of caring for plants and collecting marketable products. The relevance of developing innovative technology and technical means for removing infected potato and vegetable plants in breeding and seed production has been established. To carry out health-improving techniques for the production of vegetable and potato seeds, an innovative technology and machine have been developed for removing infected potato and vegetable crop plants in breeding and seed-growing plantings, providing movement across the field using machine vision technologies with the identification of an infected plant or a plant that does not correspond to the varietal characteristics with its subsequent removal. In the process of the research (2021-2022), a morphological matrix for selecting technical means of using functioning elements for implementing innovative technology for varietal and phytocleaning of vegetable crops and potatoes, as well as the theoretical foundations of innovative technology for removing contaminated biological objects, were developed. An indicator of the effectiveness of the implementation of innovative phytotype cleaning technology has been identified, taking into account the parameters of economic and agrotechnical indicators, as well as metal intensity, energy intensity, environmental friendliness and reliability. Analytical studies of machine technology and technical means for removing infected vegetable and potato plants are presented. A substantiation of the innovative technology for varietal and phytocleaning of vegetable crops and potatoes has been carried out, in terms of the exclusion of an unmanned aerial vehicle in the technology for detecting infected potato plants with a qualitative assessment of the feasibility of choosing technical means when using the functioning elements of the implementation of the developed technology according to the criteria of economic and agrotechnical assessment, as well as metal intensity, energy intensity and reliability . An assessment of the feasibility of choosing technical means for the functioning of elements of innovative technology showed that, according to a set of criteria, the process of varietal and phytocleaning of vegetable crops and potatoes is advisable to carry out without the use of an unmanned aerial vehicle, using an optical system for identifying infected plants in the design of the machine.
研究的目的是从理论上论证在马铃薯和蔬菜作物的育种和种子种植中使用机器视觉技术和机器人元件进行品种和植物清洁的过程。文章分析了检测生物物体病害的现代非破坏性方法;在数字化农业生产系统中从植物上摘除蔬菜作物果实的技术流程和机器,以及在照料植物和收集适销产品的操作中使用的机器人元素。已经确定了在育种和种子生产中开发清除受感染马铃薯和蔬菜植物的创新技术和技术手段的现实意义。为了在蔬菜和马铃薯种子生产中采用改善健康的技术,开发了一种创新技术和机器,用于清除育种和种子种植中受感染的马铃薯和蔬菜作物植株,利用机器视觉技术在田间移动,识别受感染的植株或不符合品种特征的植株,然后将其清除。在研究过程中(2021-2022 年),开发了一个形态矩阵,用于选择使用功能元素的技术 手段,以实施蔬菜作物和马铃薯品种和植物清洁创新技术,以及清除受污染生物物体创新技 术的理论基础。考虑到经济和农业技术指标参数,以及金属强度、能源强度、环境友好性和可靠性,确定了创新植物清洗技术实施效果的指标。对清除受感染蔬菜和马铃薯植株的机器技术和技术手段进行了分析研究。对蔬菜作物和马铃薯的品种和植物清洁创新技术进行了论证,在检测受感染的马铃薯植株的技术中排除了无人驾驶飞行器,并根据经济和农业技术评估标准以及金属强度、能源强度和可靠性,对使用实施所开发技术的功能要素时选择技术手段的可行性进行了定性评估。对创新技术功能要素选择技术手段的可行性评估表明,根据一套标准,蔬菜作物和马铃薯的品种和植物清洗过程最好不使用无人驾驶飞行器,而是在机器设计中使用光学系统识别受感染的植物。
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引用次数: 0
Dependence of blood coagulation activity on temperature in cows in vitro 奶牛体外血液凝固活动与温度的关系
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.90-97
L. L. Fomina, D. I. Berezina, T. Kulakova, K. E. Modanova
The paper presents the results of a study on how temperature affects the coagulation and anticoagulation processes in the hemostasis system of cows. The purpose of this work was to study the coagulation parameters of the blood of Ayrshire cows with hypo-, normo- and hyperthermia "in vitro", as well as to evaluate the possibility of using a coagulometer to characterize the activity of hemostasis and use hemostatic reactions in cattle as models for human medicine. The study was carried out in the Vologda region. The following parameters of the coagulation profile were analyzed: thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen and antithrombin III (AT-III) activity. Results showed that at 43 °C hypercoagulation of the blood occurred, which in clotting methods manifests itself as an acceleration of TT by 61 % (27 sec), PT by 29 % (10 sec), a prolongation of APTT by 38 % (25 sec) and a decrease in Antithrombin III activity by 51 % (5 sec) compared with normothermia. At 18 °C, hypocoagulation was detected, accompanied by an elongation of all indicators characterizing the rate of clot formation: TT by 10 % (5 sec), PT by 61 % (55 sec), APTT by 83 % (289 sec). Fibrinogen activity decreased by 82 % (106 sec), and AT-III activity increased by 85 % (56 sec) compared to normothermy. The correlation between hemostasis parameters and the temperature factor can be characterized as negative moderate (APTT), noticeable (TV, fibrinogen) and close (PT, AT-III). Using a medical coagulometer caused reaction rates to differ significantly from those occurring at the body temperature of these animals when studying the coagulation mechanisms of cows. This does not allow to recommend this device for an adequate assessment of hemostatic reactions in cattle.
本文介绍了一项关于温度如何影响奶牛止血系统凝血和抗凝过程的研究结果。这项工作的目的是研究 "体外 "低温、正常温度和高热情况下艾尔夏奶牛血液的凝血参数,以及评估使用凝血仪确定止血活动特征和将牛的止血反应作为人类医学模型的可能性。研究在沃洛格达地区进行。分析了凝血曲线的以下参数:凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原和抗凝血酶 III(AT-III)活性。结果显示,与正常体温相比,43 °C时血液出现高凝状态,在凝血方法中表现为TT加速61%(27秒),PT加速29%(10秒),APTT延长38%(25秒),抗凝血酶III活性降低51%(5秒)。在 18 °C时,检测到凝血功能减弱,血凝块形成速度的所有指标均延长:TT延长10%(5秒),PT延长61%(55秒),APTT延长83%(289秒)。与常温相比,纤维蛋白原活性降低了 82 %(106 秒),AT-III 活性增加了 85 %(56 秒)。止血参数与温度因素之间的相关性可分为中度负相关(APTT)、明显相关(TV、纤维蛋白原)和密切相关(PT、AT-III)。在研究奶牛的凝血机制时,使用医用凝血仪会导致反应速率与这些动物在体温下发生的反应速率存在显著差异。因此,不能推荐使用这种设备来充分评估牛的止血反应。
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引用次数: 0
Malignant catarrhal fever: pathogenesis, problems of prevention and control (review) 恶性卡他热:发病机制、预防和控制问题(综述)
Pub Date : 2024-02-28 DOI: 10.30766/2072-9081.2024.25.1.18-26
S. S. Spitsyna, O. Burova, P. A. Andriyanov, P. A. Zhurilov, E. A. Liskova, O. I. Zakharova, A. I. Tutrina, D. D. Kashina, N. A. Grebnev, V. N. Kachan, I. V. Yashin, A. A. Blokhin
Malignant catarrhal fever is a non-contagious infectious disease that occurs sporadically in the population of cattle and wild artiodactyls. The source of the causative agent of the disease is wildebeest, sheep, and goats, in which the infectious process is usually asymptomatic. The manifestation of clinical and pathological signs is variable, often characterized by damage to the nervous, respiratory and digestive systems, in the organs and tissues of which signs of lymphocytic and histiocytic vasculitis are noted. Diagnosis of the disease is carried out comprehensively using clinical and histological methods, but the main method is the detection of viral DNA in the material using PCR. However, control and prevention of the disease have a number of difficulties due to insufficient efficiency of diagnostic measures, lack of vaccines and outdated regulatory framework. There are no commercially available kits for diagnosing the disease in Russia. This diagnosis is available to research organizations, where complex research protocols are used. Due to insufficient knowledge of the pathogenesis, attempts to create effective vaccines against malignant catarrhal fever have not yet been successful, which does not allow for the full implementation of preventive measures. Regulatory acts defining approaches to control and elimination of the disease have not been updated for more than 20 years. The complex of these problems significantly distorts the objective epizootic situation on malignant catarrhal fever and contributes to its hidden spread with unclear economic and economic damage.
恶性卡他热是一种非传染性传染病,偶发于牛和野生半齿兽。该病的病原体来源于野马、绵羊和山羊,其感染过程通常无症状。临床和病理症状的表现多种多样,通常以神经、呼吸和消化系统受损为特征,在器官和组织中会发现淋巴细胞和组织细胞血管炎的迹象。该病的诊断可通过临床和组织学方法综合进行,但主要方法是使用 PCR 检测材料中的病毒 DNA。然而,由于诊断措施效率不高、缺乏疫苗和监管框架过时,该疾病的控制和预防存在诸多困难。俄罗斯目前还没有用于诊断该疾病的商用试剂盒。只有采用复杂研究方案的研究机构才能进行诊断。由于对发病机理了解不足,试图研制有效疫苗预防恶性卡他热的努力尚未取得成功,因此无法全面实施预防措施。20 多年来,确定控制和消除该疾病的方法的法规一直没有更新。这些问题的复杂性极大地扭曲了恶性卡他热的客观流行状况,助长了恶性卡他热的隐性传播,造成了不明确的经济和经济损失。
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引用次数: 0
Realizing the genetic potential of black-and-white cows of different genotypes by the kappa-casein gene 通过卡帕酪蛋白基因实现不同基因型黑白花奶牛的遗传潜力
Pub Date : 2023-12-16 DOI: 10.30766/2072-9081.2023.24.6.1021-1028
A. Lemyakin, L. S. Badanina, K. Sabetova, A. A. Chaitskiy, P. O. Schiogolev
Milk quality indicators are one of the main components of the breeding value of dairy cattle. The experience of many countries demonstrates that the Holsteinization of local cattle breeds is accompanied by an increase in the volume of milk produced while reducing its quality characteristics in particular to its cheese suitability. Solving this problem only by means of traditional breeding is difficult but it is possible to facilitate this task with application of modern molecular genetic methods and marker-oriented selection. The objective of the research was to assess the genetic potential of dairy performance of cattle with different genotypes by the kappa-casein gene (CSN3). The study was conducted on 104 purebred Russian black-and- white cows selected from the herds of the Yakovlevskoye APC and Raslovskoye APC. It was determined that the desired CSN3BB genotype was most common in 61 % of animals. As part of the study, the comparison of the experimental animals' own milk productivity with the productivity of their ancestors was carried out which made it possible to assess their genetic potential. The analysis showed that in the Yakovlevskoye APC, the protein content in milk in carriers of the CSN3AA genotype was significantly less than in carriers of the CSN3AB (P < 0.05) and CSNBB (P < 0.001) genotypes. There were no statistically significant differences in other productivity indicators, although the carriers of the CSNAB genotype (100.9%) differed in the greatest realization of the genetic potential in terms of the mass fraction of fat in milk, and cows with the CSNBB genotype (106.7 and 103.9 % respectively) in milk yield and protein content in milk. In the Raslovskoye APC, the greatest realization of the genetic potential in milk yield (119.5 %) was distinguished by carriers of the CSNAA genotype and the greater potential of fat and protein in milk were shown by animals with the CSNBB genotype (112.3 % and 103.7 %, respectively). Thus, the polymorphism of bovine kappa-casein gene has a significant impact both on the protein content in milk and on the realization of the genetic potential for this indicator of productivity. The obtained research results are important for breeding work with Russian black-and-white cattle aimed at improving the cheese suitability of milk.
牛奶质量指标是奶牛育种价值的主要组成部分之一。许多国家的经验表明,当地牛种的荷斯坦化伴随着牛奶产量的增加,但牛奶的质量特性,尤其是奶酪适宜性却有所下降。仅靠传统育种方法很难解决这一问题,但应用现代分子遗传方法和以标记为导向的选择则有可能促进这一任务的完成。这项研究的目的是通过卡帕酪蛋白基因(CSN3)评估不同基因型的牛在乳制品性能方面的遗传潜力。研究对象是从 Yakovlevskoye APC 和 Raslovskoye APC 牛群中挑选出的 104 头纯种俄罗斯黑白花奶牛。研究结果表明,61%的奶牛具有理想的 CSN3BB 基因型。作为研究的一部分,对实验动物自身的产奶量与其祖先的产奶量进行了比较,从而评估了它们的遗传潜力。分析表明,在雅科夫列夫斯科耶(Yakovlevskoye)APC中,CSN3AA基因型携带者牛奶中的蛋白质含量明显低于CSN3AB(P < 0.05)和CSNBB(P < 0.001)基因型携带者。尽管CSNAB基因型携带者(100.9%)与CSNBB基因型携带者(分别为106.7%和103.9%)在产奶量和牛奶蛋白质含量方面的遗传潜力发挥方面差异最大,但其他生产性能指标在统计学上没有显著差异。在 Raslovskoye APC 中,CSNAA 基因型携带者的产奶量遗传潜力最大(119.5%),CSNBB 基因型的奶牛牛奶中脂肪和蛋白质的潜力更大(分别为 112.3% 和 103.7%)。因此,牛卡帕酪蛋白基因的多态性对牛奶中蛋白质含量和实现这一生产指标的遗传潜力都有显著影响。这些研究成果对俄罗斯黑白牛的育种工作具有重要意义,旨在提高牛奶的奶酪适宜性。
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引用次数: 0
Complex bioconversion of secondary products of processing pea flour into fodder yeast 将加工豌豆粉的次生产品复合生物转化为饲料酵母
Pub Date : 2023-12-16 DOI: 10.30766/2072-9081.2023.24.6.1007-1020
V. Kolpakova, R. Ulanova, D. Kulikov, V. Gulakova
The purpose of the research is to develop a comprehensive bioconversion of secondary processing products (SPP) of pea flour: liquid whey and insoluble starch-protein residue (ISPR), formed during the isolation of food protein concentrate (PC) using various enzyme preparations. The research was carried out in 2021-2023. The bioconversion of whey and ISPR into fodder yeast (FY) was performed with the association of cultures of S. cerevisiae 121 and G. candidum 977 (1:1). There was created a mathematical model of the dependence of biomass growth on whey on technological factors, and the optimal parameters were determined: pH, temperature, amount of inoculum. In the process of biomass synthesis microorganisms absorbed glucose, xylose, galactose, fructose from whey. The parameters of ISPR hydrolysis and the conditions for its joint bioconversion with whey with the same microorganisms were established: the amount of residue added to the mass of whey, pH, temperature, process duration, pressure. With insoluble starch-protein residue hydrolyzed at pH 1.8, the amount of protein in the biomass increased by 2.2 times, reducing sugars – by 6.1 times, compared with the original whey. At the same time, glucose and maltose were absorbed. Dry FYs contained 51.09-61.68 % protein, 2-8 % lipids, and ash content 5-8 % per dry matter. The amino acid score of proteins was 90-247 %, the ratio of saturated (23.5 %) and unsaturated fatty acids (71.67 %) – 1:3, omega-6 fatty acids – 19.73 %, trans-isomers – 5.0 %. Yeast is rich in sodium, potassium, calcium, magnesium, zinc. The mass fraction of nucleic acids and heavy metals in FYs were within the normal range, in vitro digestibility was 85.73-89.74 %. The data indicated the expediency of utilization of pea flour secondary processing products into high-quality FY by comprehensive bioconversion.
该研究的目的是利用各种酶制剂,对豌豆粉的二次加工产品(SPP):液态乳清和不溶性淀粉蛋白残渣(ISPR)(在分离食品浓缩蛋白(PC)的过程中形成)进行全面的生物转化。这项研究于 2021-2023 年进行。通过将 S. cerevisiae 121 和 G. candidum 977(1:1)的培养物结合在一起,将乳清和 ISPR 生物转化为饲料酵母(FY)。建立了乳清生物量增长与技术因素相关性的数学模型,并确定了最佳参数:pH 值、温度、接种量。在生物质合成过程中,微生物从乳清中吸收葡萄糖、木糖、半乳糖和果糖。确定了 ISPR 的水解参数以及用相同微生物将其与乳清进行联合生物转化的条件:添加到乳清质量中的残渣量、pH 值、温度、过程持续时间、压力。与原始乳清相比,在 pH 值为 1.8 的条件下水解不溶性淀粉蛋白残渣后,生物质中的蛋白质含量增加了 2.2 倍,还原糖增加了 6.1 倍。同时,葡萄糖和麦芽糖也被吸收。干 FY 含有 51.09-61.68 % 的蛋白质、2-8 % 的脂类和 5-8 % 的灰分。蛋白质的氨基酸含量为 90-247%,饱和脂肪酸(23.5%)和不饱和脂肪酸(71.67%)的比例为 1:3,ω-6 脂肪酸为 19.73%,反式异构体为 5.0%。酵母富含钠、钾、钙、镁、锌。酵母中核酸和重金属的质量分数在正常范围内,体外消化率为 85.73-89.74%。这些数据表明,通过综合生物转化将豌豆粉二次加工产品转化为优质豌豆粉是可行的。
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引用次数: 0
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Agricultural Science Euro-North-East
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