A. S. Çinar, S. Rizvanoğlu, Müjde Eryılmaz, Betül Demirci, Alev Önder
Objective: The study aimed to determine the chemical composition and antibacterial, antibiofilm, and anti-quorum sensing activities of the essential oil of Salvia absconditiflora Greuter & Burdet (an endemic species) growing wildly in Türkiye. Material and Method: The essential oil from the aerial parts of the plant was obtained by hydro-distillation (0.4%) and analyzed by GC-FID and GC-MS. In addition, the broth microdilution method was used to determine antibacterial activity. The crystal violet assay was performed for antibiofilm activity, and the reporter bacteria Chromobacterium violaceum ATCC 12472 was used in the anti-quorum sensing activity test. Result and Discussion: The major components of the essential oil were identified as 1,8-cineole (32.2%), camphor (13.6%), α-pinene (7.6%), camphene (5.5%), and viridiflorol (5.1%). The essential oil showed the best antibacterial activity against Gram-positive test bacteria, with a minimum inhibitory concentration (MIC) of 0.0078 (v/v) against Staphylococcus aureus strains. The percentage biofilm inhibition value of the essential oil was determined as 84.4%. The inhibition of violacein production by the essential oil in Chromobacterium violaceum ATCC 12472 indicated the possibility of anti-quorum sensing activity. The results of this study show that the essential oil of S. absconditiflora could be a promising alternative in fighting bacterial infections.
{"title":"CHEMICAL COMPOSITION AND BIOACTIVITIES OF ESSENTIAL OIL FROM AN ENDEMIC SALVIA ABSCONDITIFLORA GREUTER & BURDET","authors":"A. S. Çinar, S. Rizvanoğlu, Müjde Eryılmaz, Betül Demirci, Alev Önder","doi":"10.33483/jfpau.1416623","DOIUrl":"https://doi.org/10.33483/jfpau.1416623","url":null,"abstract":"Objective: The study aimed to determine the chemical composition and antibacterial, antibiofilm, and anti-quorum sensing activities of the essential oil of Salvia absconditiflora Greuter & Burdet (an endemic species) growing wildly in Türkiye.\u0000Material and Method: The essential oil from the aerial parts of the plant was obtained by hydro-distillation (0.4%) and analyzed by GC-FID and GC-MS. In addition, the broth microdilution method was used to determine antibacterial activity. The crystal violet assay was performed for antibiofilm activity, and the reporter bacteria Chromobacterium violaceum ATCC 12472 was used in the anti-quorum sensing activity test.\u0000Result and Discussion: The major components of the essential oil were identified as 1,8-cineole (32.2%), camphor (13.6%), α-pinene (7.6%), camphene (5.5%), and viridiflorol (5.1%). The essential oil showed the best antibacterial activity against Gram-positive test bacteria, with a minimum inhibitory concentration (MIC) of 0.0078 (v/v) against Staphylococcus aureus strains. The percentage biofilm inhibition value of the essential oil was determined as 84.4%. The inhibition of violacein production by the essential oil in Chromobacterium violaceum ATCC 12472 indicated the possibility of anti-quorum sensing activity. The results of this study show that the essential oil of S. absconditiflora could be a promising alternative in fighting bacterial infections.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"56 43","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140662086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Alzheimer Hastalığı hem ülkemizde hem de dünya genelinde, yaş ortalamasının da artması ile birlikte görülme sıklığı her geçen gün artan ilerleyici ve zorlu bir hastalıktır. Hastalığa yakalanma nedenleri ve hastalığın patolojisi hala tam olarak aydınlatılamamış, hastalığa yakalanmayı önleyen bir yol bulunamamış ve hasta olduktan sonra da kullanıldığı takdirde hastayı tamamen iyileştirdiği kanıtlanmış bir molekül keşfedilememiştir. Konvansiyonel ilaçlar ile tedavi halen daha klinikte en çok başvurulan ve sadece semptomatik yarar sağlayan tedavi yöntemidir. Günümüzde innovatif ilaç çalışmaları Alzheimer Hastalığına ışık olabilmek için devam etmektedir. Sonuç ve Tartışma: Hastalığın patofizyolojisi tam olarak anlaşılamadan tedavi edilmesi mümkün olmamakla birlikte gelişen ilaç teknolojisi ile umut vaat eden yeni moleküller klinikte kullanıma sunulmuştur. Etkili ve güvenli bulunmalarının devamı halinde ilaç pazarında yerini sağlamlaştırarak hastalara umut olacaklardır.
{"title":"ALZHEIMER HASTALIĞI, RİSK FAKTÖRLERİ VE TEDAVİ","authors":"Nejla Yildirim, Binay Can Eke","doi":"10.33483/jfpau.1441827","DOIUrl":"https://doi.org/10.33483/jfpau.1441827","url":null,"abstract":"Amaç: Alzheimer Hastalığı hem ülkemizde hem de dünya genelinde, yaş ortalamasının da artması ile birlikte görülme sıklığı her geçen gün artan ilerleyici ve zorlu bir hastalıktır. Hastalığa yakalanma nedenleri ve hastalığın patolojisi hala tam olarak aydınlatılamamış, hastalığa yakalanmayı önleyen bir yol bulunamamış ve hasta olduktan sonra da kullanıldığı takdirde hastayı tamamen iyileştirdiği kanıtlanmış bir molekül keşfedilememiştir. Konvansiyonel ilaçlar ile tedavi halen daha klinikte en çok başvurulan ve sadece semptomatik yarar sağlayan tedavi yöntemidir. Günümüzde innovatif ilaç çalışmaları Alzheimer Hastalığına ışık olabilmek için devam etmektedir.\u0000Sonuç ve Tartışma: Hastalığın patofizyolojisi tam olarak anlaşılamadan tedavi edilmesi mümkün olmamakla birlikte gelişen ilaç teknolojisi ile umut vaat eden yeni moleküller klinikte kullanıma sunulmuştur. Etkili ve güvenli bulunmalarının devamı halinde ilaç pazarında yerini sağlamlaştırarak hastalara umut olacaklardır.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140688661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study investigated the anticancer effects of nucleoside and non-nucleoside reverse transcriptase inhibitors drugs by computational methods. The study aimed to evaluate the binding capacity of these drugs on the telomerase essential N-terminal (TEN) domain of telomerase reverse transcriptase (TERT). Molecular docking was used to assess the drugs' binding potential to the TEN domain. The stability of the protein-drug combination obtained from the docking method was assessed using molecular dynamics (MD) modeling. Material and Method: The TEN domain of TERT's crystal structure was obtained from the Protein Data Bank (PDB). The crystal structure identified by the PDB code 2B2A has a resolution of 2.2 Å. The molecular docking was performed using AutoDock Vina. The complexes were visualized using Biovia Discovery Studio. The MD simulation was conducted using GROMACS 2020 as indicated. An MD simulation was conducted for 200 ns on both the complexes and the free protein. The RMSD (root mean square deviation) of the protein and the molecules in relation to the protein, RMSF (root mean square fluctuation), and Rg (radius of gyration) were shown via Qt Grace. Result and Discussion: Doravirine, Etravirine, Rilpivirine showed higher binding affinity to the TEN domain compared to the reference TERT inhibitor, BIBR1532, based on the docking investigation. The MD simulation analysis showed that the protein-Doravirine complex had the highest stability in remaining within the protein's binding pocket. On the contrary, the protein-Rilpivirine complex decreased stability, potentially causing the ligand to not stay within the binding site. Doravirine was found to inhibit the TEN domain in the computational study. Therefore, the design and synthesis of novel doravirin derivatives is being considered because of the potential anticancer activity of doravirin in inhibiting the TEN domain of TERT.
{"title":"MOLECULAR DOCKING AND MOLECULAR DYNAMICS SIMULATIONS INHIBITION AGAINST OF HUMAN TELOMERASE BY NUCLEOSIDE AND NON-NUCLEOSIDE REVERSE TRANSCRIPTASE INHIBITORS (NRTIs/NNRTIs)","authors":"Dilan Konyar, Muhammed Tılahun Muhammed","doi":"10.33483/jfpau.1444259","DOIUrl":"https://doi.org/10.33483/jfpau.1444259","url":null,"abstract":"Objective: This study investigated the anticancer effects of nucleoside and non-nucleoside reverse transcriptase inhibitors drugs by computational methods. The study aimed to evaluate the binding capacity of these drugs on the telomerase essential N-terminal (TEN) domain of telomerase reverse transcriptase (TERT). Molecular docking was used to assess the drugs' binding potential to the TEN domain. The stability of the protein-drug combination obtained from the docking method was assessed using molecular dynamics (MD) modeling. \u0000Material and Method: The TEN domain of TERT's crystal structure was obtained from the Protein Data Bank (PDB). The crystal structure identified by the PDB code 2B2A has a resolution of 2.2 Å. The molecular docking was performed using AutoDock Vina. The complexes were visualized using Biovia Discovery Studio. The MD simulation was conducted using GROMACS 2020 as indicated. An MD simulation was conducted for 200 ns on both the complexes and the free protein. The RMSD (root mean square deviation) of the protein and the molecules in relation to the protein, RMSF (root mean square fluctuation), and Rg (radius of gyration) were shown via Qt Grace.\u0000Result and Discussion: Doravirine, Etravirine, Rilpivirine showed higher binding affinity to the TEN domain compared to the reference TERT inhibitor, BIBR1532, based on the docking investigation. The MD simulation analysis showed that the protein-Doravirine complex had the highest stability in remaining within the protein's binding pocket. On the contrary, the protein-Rilpivirine complex decreased stability, potentially causing the ligand to not stay within the binding site. Doravirine was found to inhibit the TEN domain in the computational study. Therefore, the design and synthesis of novel doravirin derivatives is being considered because of the potential anticancer activity of doravirin in inhibiting the TEN domain of TERT.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140694187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heybet Kerem Polat, Eren Aytekin, Nasıf Fatih Karakuyu, Nihat Kurt, Yonca Yazıksız
Amaç: Bu derleme kapsamında, oküler kullanımdaki katı lipit nanopartikül (KLN) ve nanoyapılı lipit taşıyıcı (NLT) sistemlerine, bu formülasyonların sterilizasyonuna ve tasarımla kalite (QbD) hakkında yapılan son araştırmaları tartışmak amaçlanmıştır. Sonuç ve Tartışma: Göze ilaç taşınması son yıllardaki gelişmelere rağmen hala karmaşık bir sorun olarak devem etmekte ve etkili ilaç taşınabilmesi için yenilikçi yaklaşımlara ihtiyaç duyulan bir alandır. Bu derlemede, yenilikçi yaklaşımlardan olan KLN’ler ve NLT’lerin oftalmik ilaç uygulamalarında sağladıkları üstünlükler güncel literatür örnekleriyle tartışılmıştır. KLN’ler oda sıcaklığında katı halde bulunan lipitlerin genellikle eritilmesi ya da çeşitli solvanlarda çözündürülmesi ile hazırlanan lipit partiküllerdir. NLT’ler ise yapısında katı lipitlerle birlikte oda sıcaklığında sıvı halde bulunan lipitleri de içermektedir. Oküler ilaç uygulamalarında kritik aşamalardan biri de sterilizasyon basamağıdır. Uygun sterilizasyon işleminin seçiminde, kullanılan lipitlerin erime dereceleri, serbest radikal oluşturma eğilimleri ve partikül büyüklükleri göz önünde bulundurulmalıdır. Sonuç olarak, KLN ve NLT’ler hem biyouyumluluk hem de etkililik anlamında oküler tedaviler için umut vadeden ilaç taşıyıcı sistemlerdir. Derleme kapsamında incelenen literatür çalışmaları da bu çıkarımı desteklemektedir. Ancak saklama süresince karşılaşılabilen stabilite sorunları ve tekrarlanabilir büyük ölçekte üretim konusunda yaşanan sıkıntılar nedeniyle klinik tedavide yeterince kullanılamamaktadır. Bu sorunların çözümü aşamasında QbD’nin etkili olacağı düşünülmektedir.
{"title":"OKÜLER İLAÇ TAŞIYICI SİSTEM OLARAK LİPİT BAZLI NANOPARTİKÜLLER","authors":"Heybet Kerem Polat, Eren Aytekin, Nasıf Fatih Karakuyu, Nihat Kurt, Yonca Yazıksız","doi":"10.33483/jfpau.1420064","DOIUrl":"https://doi.org/10.33483/jfpau.1420064","url":null,"abstract":"Amaç: Bu derleme kapsamında, oküler kullanımdaki katı lipit nanopartikül (KLN) ve nanoyapılı lipit taşıyıcı (NLT) sistemlerine, bu formülasyonların sterilizasyonuna ve tasarımla kalite (QbD) hakkında yapılan son araştırmaları tartışmak amaçlanmıştır.\u0000Sonuç ve Tartışma: Göze ilaç taşınması son yıllardaki gelişmelere rağmen hala karmaşık bir sorun olarak devem etmekte ve etkili ilaç taşınabilmesi için yenilikçi yaklaşımlara ihtiyaç duyulan bir alandır. Bu derlemede, yenilikçi yaklaşımlardan olan KLN’ler ve NLT’lerin oftalmik ilaç uygulamalarında sağladıkları üstünlükler güncel literatür örnekleriyle tartışılmıştır. KLN’ler oda sıcaklığında katı halde bulunan lipitlerin genellikle eritilmesi ya da çeşitli solvanlarda çözündürülmesi ile hazırlanan lipit partiküllerdir. NLT’ler ise yapısında katı lipitlerle birlikte oda sıcaklığında sıvı halde bulunan lipitleri de içermektedir. Oküler ilaç uygulamalarında kritik aşamalardan biri de sterilizasyon basamağıdır. Uygun sterilizasyon işleminin seçiminde, kullanılan lipitlerin erime dereceleri, serbest radikal oluşturma eğilimleri ve partikül büyüklükleri göz önünde bulundurulmalıdır. Sonuç olarak, KLN ve NLT’ler hem biyouyumluluk hem de etkililik anlamında oküler tedaviler için umut vadeden ilaç taşıyıcı sistemlerdir. Derleme kapsamında incelenen literatür çalışmaları da bu çıkarımı desteklemektedir. Ancak saklama süresince karşılaşılabilen stabilite sorunları ve tekrarlanabilir büyük ölçekte üretim konusunda yaşanan sıkıntılar nedeniyle klinik tedavide yeterince kullanılamamaktadır. Bu sorunların çözümü aşamasında QbD’nin etkili olacağı düşünülmektedir.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"5 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140697645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Job analysis is a method in which the duties and responsibilities in an organization are examined and researched systematically. The aim of this study is to determine both the workload of the pharmacist including the consultation to the patients and administrative works in community pharmacies, and the time allocated to these responsibilities. We are presenting advices which increase the productivity of the management. Material and Method: This study is descriptive-survey research. In this research, the survey method was used as a data collection tool. A questionnaire was prepared for the study, which was created by conducting interviews identifying the tasks that the pharmacists do in their routine. The survey was applied to a small number of pharmacists. The final version was sent by e-mail to pharmacists located in Ankara. Result and Discussion: It was determined that pharmacists spend most of their time in the pharmacy on patient counseling. Pharmacy staff assist the pharmacist with counseling, but mostly with administrative work. This study is important for the students who plan to be community pharmacists to be able to manage their pharmacies efficiently. It shows that the work awaits them in a community pharmacy and the time to do the work in the pharmacy on average basis.
{"title":"A STUDY ON JOB ANALYSIS IN COMMUNITY PHARMACIES: CASE OF ANKARA","authors":"Mine Zanbak Canan, M. Uzun, Gülbin Özçelikay","doi":"10.33483/jfpau.1403130","DOIUrl":"https://doi.org/10.33483/jfpau.1403130","url":null,"abstract":"Objective: Job analysis is a method in which the duties and responsibilities in an organization are examined and researched systematically. The aim of this study is to determine both the workload of the pharmacist including the consultation to the patients and administrative works in community pharmacies, and the time allocated to these responsibilities. We are presenting advices which increase the productivity of the management.\u0000Material and Method: This study is descriptive-survey research. In this research, the survey method was used as a data collection tool. A questionnaire was prepared for the study, which was created by conducting interviews identifying the tasks that the pharmacists do in their routine. The survey was applied to a small number of pharmacists. The final version was sent by e-mail to pharmacists located in Ankara.\u0000Result and Discussion: It was determined that pharmacists spend most of their time in the pharmacy on patient counseling. Pharmacy staff assist the pharmacist with counseling, but mostly with administrative work. This study is important for the students who plan to be community pharmacists to be able to manage their pharmacies efficiently. It shows that the work awaits them in a community pharmacy and the time to do the work in the pharmacy on average basis.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140705916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aims to reveal how the restrictions, quarantines, social policies, and implemented measures during the COVID-19 pandemic have affected the consumption of antidepressant drugs in Türkiye. Material and Method: The research was conducted based on the total consumption figures of antidepressant drugs between 2017 and 2022. The necessary data were obtained from IQVIA/Turkey and OECD official sources. Result and Discussion: Our study has shown that antidepressant consumption in Türkiye during the pandemic has increased much more than expected. Additionally, alongside countries where consumption has increased in OECD countries, there are countries where consumption has not been affected. Examining countries' social and economic policies where consumption is unaffected during the pandemic would be beneficial. Türkiye's social and economic policies have proven inadequate in psychologically protecting the country's citizens during the pandemic.
{"title":"ANTIDEPRESSANT CONSUMPTION IN TÜRKİYE DURING THE PANDEMIC","authors":"Hilal Demirhan Keleş, Emrah Bilgener, Emre Keleş","doi":"10.33483/jfpau.1431730","DOIUrl":"https://doi.org/10.33483/jfpau.1431730","url":null,"abstract":"Objective: This study aims to reveal how the restrictions, quarantines, social policies, and implemented measures during the COVID-19 pandemic have affected the consumption of antidepressant drugs in Türkiye.\u0000Material and Method: The research was conducted based on the total consumption figures of antidepressant drugs between 2017 and 2022. The necessary data were obtained from IQVIA/Turkey and OECD official sources.\u0000Result and Discussion: Our study has shown that antidepressant consumption in Türkiye during the pandemic has increased much more than expected. Additionally, alongside countries where consumption has increased in OECD countries, there are countries where consumption has not been affected. Examining countries' social and economic policies where consumption is unaffected during the pandemic would be beneficial. Türkiye's social and economic policies have proven inadequate in psychologically protecting the country's citizens during the pandemic.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140729486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Petroselinum crispum (Mill.) Fuss is a bright green biennial medicinal and aromatic herb that grows almost all over the world. Today, it is one of the most commonly used culinary herbs. In addition to its use as food, it has been shown to possess broad pharmacological activities in several in vivo and in vitro studies. This study aimed to comprehensively summarize the current studies on the traditional use, phytochemical composition, pharmacological activities, clinical studies, toxicity, and drug interactions of parsley. Result and Discussion: According to the literature data, parsley is used as a diuretic, carminative, emmenagogue and for the prevention and treatment of kidney stone formation, the treatment of conditions such as urinary tract infections and stomach disorders. Its phytochemical composition consists of flavonoids, coumarins, phenolic compounds, organic acids, carotenoids, vitamins, minerals, fixed oil, essential oil, and other compounds. Studies on P. crispum have shown that it has a wide range of pharmacological activities, including antioxidant, antibacterial, antifungal, antidiabetic, antihypertensive, antiplatelet, analgesic, antiinflammatory, antihepatotoxic, antinephrotoxic, anticancer, antiurolithiatic, wound healing, antiobesity, estrogenic and neuroprotective effects. This review comprehensively summarizes the scientific data of the last ten years (2013-2023) on P. crispum.
{"title":"PETROSELINUM CRISPUM (MILL.) FUSS (PARSLEY), A FOOD AND MEDICINALLY IMPORTANT PLANT: A REVIEW OF RECENT STUDIES BETWEEN 2013-2023","authors":"Tuğba Subaş, U. Özgen, İçim Gökkaya, Gülin Renda","doi":"10.33483/jfpau.1362626","DOIUrl":"https://doi.org/10.33483/jfpau.1362626","url":null,"abstract":"Objective: Petroselinum crispum (Mill.) Fuss is a bright green biennial medicinal and aromatic herb that grows almost all over the world. Today, it is one of the most commonly used culinary herbs. In addition to its use as food, it has been shown to possess broad pharmacological activities in several in vivo and in vitro studies. This study aimed to comprehensively summarize the current studies on the traditional use, phytochemical composition, pharmacological activities, clinical studies, toxicity, and drug interactions of parsley.\u0000Result and Discussion: According to the literature data, parsley is used as a diuretic, carminative, emmenagogue and for the prevention and treatment of kidney stone formation, the treatment of conditions such as urinary tract infections and stomach disorders. Its phytochemical composition consists of flavonoids, coumarins, phenolic compounds, organic acids, carotenoids, vitamins, minerals, fixed oil, essential oil, and other compounds. Studies on P. crispum have shown that it has a wide range of pharmacological activities, including antioxidant, antibacterial, antifungal, antidiabetic, antihypertensive, antiplatelet, analgesic, antiinflammatory, antihepatotoxic, antinephrotoxic, anticancer, antiurolithiatic, wound healing, antiobesity, estrogenic and neuroprotective effects. This review comprehensively summarizes the scientific data of the last ten years (2013-2023) on P. crispum.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"279 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140730290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ekin Erdoğmuş, Seda Ipek, Belma Gümüşel, Y. Duydu, Aylin Üstündağ
Objective: This study aimed to determine the DNA damage induced by Bisphenol A (BPA) and Bisphenol S (BPS) on MCF7 cell line. Material and Method: DNA damage was determined by COMET assay in MCF7 cell line at 0.1, 0.5, 1, 5, 10 and 50 μM concentrations of BPA and BPS. Result and Discussion: All BPA and BPS concentrations studied (0.1, 0.5, 1, 5, 10 and 50 μM) significantly induced DNA damage on MCF7 cell line compared with control (p<0.05). BPS significantly induced DNA damage more than BPA at the 3 highest concentrations studied (5, 10 and 50 μM) (p<0.05). This study shows that bisphenol derivatives can also cause DNA damage like BPA.
研究目的本研究旨在确定双酚 A（BPA）和双酚 S（BPS）对 MCF7 细胞株 DNA 的损伤：采用 COMET 法测定 MCF7 细胞株在 0.1、0.5、1、5、10 和 50 μM 双酚 A 和双酚 S 浓度下的 DNA 损伤：与对照组相比，所有研究浓度（0.1、0.5、1、5、10 和 50 μM）的双酚 A 和双酚 BPS 都能明显诱导 MCF7 细胞系的 DNA 损伤（p<0.05）。在研究的 3 个最高浓度（5、10 和 50 μM）下，双酚 A 诱导的 DNA 损伤明显高于双酚 BPA（p<0.05）。这项研究表明，双酚衍生物也会像双酚 A 一样造成 DNA 损伤。
{"title":"DETERMINATION OF DNA DAMAGE INDUCED BY BISPHENOL A AND BISPHENOL S IN MCF7 CELL LINE","authors":"Ekin Erdoğmuş, Seda Ipek, Belma Gümüşel, Y. Duydu, Aylin Üstündağ","doi":"10.33483/jfpau.1428739","DOIUrl":"https://doi.org/10.33483/jfpau.1428739","url":null,"abstract":"Objective: This study aimed to determine the DNA damage induced by Bisphenol A (BPA) and Bisphenol S (BPS) on MCF7 cell line.\u0000Material and Method: DNA damage was determined by COMET assay in MCF7 cell line at 0.1, 0.5, 1, 5, 10 and 50 μM concentrations of BPA and BPS.\u0000Result and Discussion: All BPA and BPS concentrations studied (0.1, 0.5, 1, 5, 10 and 50 μM) significantly induced DNA damage on MCF7 cell line compared with control (p<0.05). BPS significantly induced DNA damage more than BPA at the 3 highest concentrations studied (5, 10 and 50 μM) (p<0.05). This study shows that bisphenol derivatives can also cause DNA damage like BPA.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140730048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Bu araştırma makalesinin amacı, terapötik müdahale için yeni yollar keşfetmek üzere in-silico yöntemler kullanarak anti-apoptotik protein inhibitörleri incelemektir. Bununla birlikte ilaç keşif süreçlerini hızlandırmada hesaplamalı yaklaşımların potansiyelini vurgulamakta ve daha fazla deneysel doğrulama için umut verici adayları belirlemek için uygun maliyetli ve zaman açısından verimli bir strateji sunmayı hedeflemektedir. Gereç ve Yöntem: Bu çalışmada, önemli anti-apoptotik proteinleri hedef alan potansiyel inhibitörleri belirlemek için gelişmiş hesaplama teknikleri kullanılmıştır. Moleküler yerleştirmeden yararlanarak, hedef proteinlerle bağlanma ilgileri ve etkileşim modellerini tespit etmek için çeşitli kimyasal kütüphaneleri sistematik olarak taranmıştır Çalışma, potansiyel ilaç adaylarının tasarımını optimize etmek için bu etkileşimlerin yapısal inceliklerini aydınlatmaya odaklanmıştır. Sonuç ve Tartışma: Bulgularımız, yüksek bağlanma afiniteleri ve uygun farmakokinetik özelliklere sahip umut verici öncü bileşikleri ortaya çıkarmakta ve bunları daha fazla deneysel doğrulama için aday olarak sunmaktadır. Bu çalışmada elde edilen hesaplamalı içgörüler, anti-apoptotik protein inhibitörleri hakkındaki artan bilgi birikimine katkıda bulunmakla kalmayıp, aynı zamanda anormal apoptotik düzenleme ile karakterize edilen hastalıklarla mücadelede yeni terapötiklerin rasyonel tasarımı için bir temel sağlamaktadır.
{"title":"ANTİ-APOPTOTİK PROTEİN İÇİN YENİ İNHİBİTÖRLERİN İN-SİLİKO YÖNTEMLERLE ARAŞTIRILMASI","authors":"M. Ünal","doi":"10.33483/jfpau.1412714","DOIUrl":"https://doi.org/10.33483/jfpau.1412714","url":null,"abstract":"Amaç: Bu araştırma makalesinin amacı, terapötik müdahale için yeni yollar keşfetmek üzere in-silico yöntemler kullanarak anti-apoptotik protein inhibitörleri incelemektir. Bununla birlikte ilaç keşif süreçlerini hızlandırmada hesaplamalı yaklaşımların potansiyelini vurgulamakta ve daha fazla deneysel doğrulama için umut verici adayları belirlemek için uygun maliyetli ve zaman açısından verimli bir strateji sunmayı hedeflemektedir.\u0000Gereç ve Yöntem: Bu çalışmada, önemli anti-apoptotik proteinleri hedef alan potansiyel inhibitörleri belirlemek için gelişmiş hesaplama teknikleri kullanılmıştır. Moleküler yerleştirmeden yararlanarak, hedef proteinlerle bağlanma ilgileri ve etkileşim modellerini tespit etmek için çeşitli kimyasal kütüphaneleri sistematik olarak taranmıştır Çalışma, potansiyel ilaç adaylarının tasarımını optimize etmek için bu etkileşimlerin yapısal inceliklerini aydınlatmaya odaklanmıştır.\u0000Sonuç ve Tartışma: Bulgularımız, yüksek bağlanma afiniteleri ve uygun farmakokinetik özelliklere sahip umut verici öncü bileşikleri ortaya çıkarmakta ve bunları daha fazla deneysel doğrulama için aday olarak sunmaktadır. Bu çalışmada elde edilen hesaplamalı içgörüler, anti-apoptotik protein inhibitörleri hakkındaki artan bilgi birikimine katkıda bulunmakla kalmayıp, aynı zamanda anormal apoptotik düzenleme ile karakterize edilen hastalıklarla mücadelede yeni terapötiklerin rasyonel tasarımı için bir temel sağlamaktadır.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"53 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140730044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In our study, it was aimed to make a comparative analysis of the environmental impact profiles of two approaches including Gas Chromatography (GC) and Liquid Chromatography (LC) methods, which are frequently used techniques for the determination of non-steroidal anti-inflammatory drugs (NSAIDs) and their metabolites in environmental water samples. Material and Method: The evaluation of the methods' environmental impact was performed using National Environmental Methods Index Label (NEMI), Analytical Eco-scale, Analytical GREEnness Metric (AGREE), and Green Analytical Procedure Index (GAPI). Result and Discussion: The routine analysis of NSAIDs in environmental waters is carried out, resulting in a significant volume of chemical waste. In recent times, there has been a growing significance attributed to environmentally conscious analytical methodologies and the evaluation of methodologies through a green lens to confront this challenge. There is no statistically significant difference in terms of environmental impact profile was observed between the two methods compared.
{"title":"EVALUATION OF GREENNESS PROFILES OF VARIOUS DEVELOPED METHODS FOR THE DETERMINATION OF COMMONLY USED NONSTEROIDAL ANTI-INFLAMMATORY DRUGS (NSAIDs) IN ENVIRONMENTAL WATERS","authors":"B. Sezgin, M. Soyseven","doi":"10.33483/jfpau.1425350","DOIUrl":"https://doi.org/10.33483/jfpau.1425350","url":null,"abstract":"Objective: In our study, it was aimed to make a comparative analysis of the environmental impact profiles of two approaches including Gas Chromatography (GC) and Liquid Chromatography (LC) methods, which are frequently used techniques for the determination of non-steroidal anti-inflammatory drugs (NSAIDs) and their metabolites in environmental water samples.\u0000Material and Method: The evaluation of the methods' environmental impact was performed using National Environmental Methods Index Label (NEMI), Analytical Eco-scale, Analytical GREEnness Metric (AGREE), and Green Analytical Procedure Index (GAPI).\u0000Result and Discussion: The routine analysis of NSAIDs in environmental waters is carried out, resulting in a significant volume of chemical waste. In recent times, there has been a growing significance attributed to environmentally conscious analytical methodologies and the evaluation of methodologies through a green lens to confront this challenge. There is no statistically significant difference in terms of environmental impact profile was observed between the two methods compared.","PeriodicalId":504701,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":"45 37","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140752347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：