Melike Bakır, Şehriban Demir, Cebrail Yıldırım, Abdullah Kahraman
Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (Lens culinaris Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition, we tested the transferability of 86 SSR markers, including the 33 newly developed ones, to other legume species. Transferability rates of lentil SSR markers to other species varied between 13.76% (Vicia sativa L.) and 2.58% (Phaseolus vulgaris L. and Trifolium pratense L.). These new SSR markers could be used in further molecular breeding, population genetics, and genetic mapping studies in lentils and other legume species.
{"title":"Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes","authors":"Melike Bakır, Şehriban Demir, Cebrail Yıldırım, Abdullah Kahraman","doi":"10.17221/57/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/57/2023-cjgpb","url":null,"abstract":"Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (<i>Lens culinaris</i> Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition, we tested the transferability of 86 SSR markers, including the 33 newly developed ones, to other legume species. Transferability rates of lentil SSR markers to other species varied between 13.76% (<i>Vicia sativa</i> L.) and 2.58% (<i>Phaseolus vulgaris</i> L. and <i>Trifolium pratense</i> L.). These new SSR markers could be used in further molecular breeding, population genetics, and genetic mapping studies in lentils and other legume species.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135367110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F2 segregating population of melons generated from these two parents was inoculated by the PM pathogen. Based on bulk segregant analysis (BSA) using these 100 InDel markers, the powdery mildew resistance was associated with the genomic region LVpm12.1 on the melon chromosome 12. This region overlapped the previously described quantitative trait locus (QTL)-hotspot area carrying multiple PM-resistance QTLs. Moreover, conventional QTL mapping analysis was done, which located LVpm12.1 in the region between 22.72 and 23.34 Mb, where three highly polymorphic InDel markers MInDel89, MInDel92, and MInDel93 were detected. Therefore, these markers could be used to track this resistance locus in melon while the lines carrying this locus could be employed in PM melon resistance breeding programs after validation tests.
{"title":"Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon","authors":"Yawo Mawunyo Nevame Adedze, Xia Lu, Wenyi Fan, Wenting Zhang, Xue Yang, Zhijun Deng, Md. Amirul Alam, Guangli Xu, Lihua Zhang, Wenhu Li","doi":"10.17221/40/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/40/2023-cjgpb","url":null,"abstract":"Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F<sub>2</sub> segregating population of melons generated from these two parents was inoculated by the PM pathogen. Based on bulk segregant analysis (BSA) using these 100 InDel markers, the powdery mildew resistance was associated with the genomic region <i>LVpm12.1</i> on the melon chromosome 12. This region overlapped the previously described quantitative trait locus (QTL)-hotspot area carrying multiple PM-resistance QTLs. Moreover, conventional QTL mapping analysis was done, which located <i>LVpm12.1</i> in the region between 22.72 and 23.34 Mb, where three highly polymorphic InDel markers MInDel89, MInDel92, and MInDel93 were detected. Therefore, these markers could be used to track this resistance locus in melon while the lines carrying this locus could be employed in PM melon resistance breeding programs after validation tests.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135830741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martin Hádlík, Miroslav Baránek, Ivana Flajšingerová, Kateřina Baránková
Fungal pathogens Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni (downy mildew) and Erysiphe necator Schwein. (powdery mildew) represent the biggest threats for grape growers worldwide. Under suitable conditions, these pathogens can spread very quickly through vineyards and cause significant damage. The most ecological way to reduce the possibility of infection in vineyards is growing interspecific genotypes which are able to suppress these pathogens and stop the spread of infection. With this in mind, 9 international and 11 Czech grapevine genotypes were analysed in order to genotype the resistance loci present in them. As a tool a set of SSR markers linked to known loci of resistance to downy and powdery mildew was used. Namely, presence of four loci responsible for resistance to Plasmopara viticola (Rpv3, Rpv4, Rpv7, and Rpv10) and two for Erysiphe necator (Ren3 and Ren9) loci were analysed with respective SSR markers. By this way the degree of resistance gene pyramiding was newly assessed in all analysed cultivars and their perspectives in grapevine breeding are discussed.
真菌病原体葡萄浆原菌(Plasmopara viticola)。& M.A.柯蒂斯)伯尔。& De Toni(霜霉病)和Erysiphe necator Schwein。白粉病是全球葡萄种植者面临的最大威胁。在适当的条件下，这些病原体可以在葡萄园中迅速传播并造成重大损害。最生态的方式toÂ降低ofÂ感染的可能性inÂ葡萄园isÂ种植种间基因型，能够toÂ抑制这些病原体并阻止传播ofÂ感染。基于这种inÂ思路，对9个国际葡萄基因型和11个捷克葡萄基因型进行了分析inÂ顺序为toÂ，对它们所存在的抗性位点进行了基因型分析inÂ。As aÂ工具a set of SSR标记链接toÂ已知位点ofÂ抗性toÂ使用霜霉病和白粉病。分别用SSR标记分析了of 4个负责抗性的位点toÂ葡萄浆原体(Rpv3、Rpv4、Rpv7和Rpv10)和2个负责抗性的位点(Ren3和Ren9)的存在。ByÂ由此对ofÂ抗性基因金字塔化程度进行了新的评价inÂ对所有分析品种及其选育前景进行了讨论inÂ。
{"title":"Verification of resistance loci pyramiding in popular interspecific grape varieties using SSR markers","authors":"Martin Hádlík, Miroslav Baránek, Ivana Flajšingerová, Kateřina Baránková","doi":"10.17221/43/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/43/2023-cjgpb","url":null,"abstract":"Fungal pathogens Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni (downy mildew) and Erysiphe necator Schwein. (powdery mildew) represent the biggest threats for grape growers worldwide. Under suitable conditions, these pathogens can spread very quickly through vineyards and cause significant damage. The most ecological way to reduce the possibility of infection in vineyards is growing interspecific genotypes which are able to suppress these pathogens and stop the spread of infection. With this in mind, 9 international and 11 Czech grapevine genotypes were analysed in order to genotype the resistance loci present in them. As a tool a set of SSR markers linked to known loci of resistance to downy and powdery mildew was used. Namely, presence of four loci responsible for resistance to Plasmopara viticola (Rpv3, Rpv4, Rpv7, and Rpv10) and two for Erysiphe necator (Ren3 and Ren9) loci were analysed with respective SSR markers. By this way the degree of resistance gene pyramiding was newly assessed in all analysed cultivars and their perspectives in grapevine breeding are discussed.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135833642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Revanasiddayya Revanasiddayya, Jayaprakash Mohan Nidagundi, B. Fakrudin, P. Kuchanur, L. N. Yogeesh, S. Hanchinal, Talagunda Chandrashekar Suma, G. Sunkad, B. Muralidhara, Doddamani Maheshkumar, Channabasava Channabasava, Patil Sudha, Shivamurthy Rashmi, Virupapura Cholaraya Raghavendra
{"title":"Genetic diversity among coloured cotton genotypes in relation to their fibre colour and ploidy level based on SSR markers","authors":"Revanasiddayya Revanasiddayya, Jayaprakash Mohan Nidagundi, B. Fakrudin, P. Kuchanur, L. N. Yogeesh, S. Hanchinal, Talagunda Chandrashekar Suma, G. Sunkad, B. Muralidhara, Doddamani Maheshkumar, Channabasava Channabasava, Patil Sudha, Shivamurthy Rashmi, Virupapura Cholaraya Raghavendra","doi":"10.17221/12/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/12/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45962210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Erbaş, H. Baydar, H. Hatipoğlu, H. Koc, M. Babaoğlu, A. Köse
{"title":"Genotypic and environmental variability and stability of seed yield, oil content and fatty acids in high-oleic and high-linoleic safflower (Carthamus tinctorius L.) lines and cultivars","authors":"S. Erbaş, H. Baydar, H. Hatipoğlu, H. Koc, M. Babaoğlu, A. Köse","doi":"10.17221/113/2022-cjgpb","DOIUrl":"https://doi.org/10.17221/113/2022-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46500540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Pavlović, J. Damnjanovic, Z. Girek, Lela Belić, M. Ugrinović
{"title":"Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli","authors":"S. Pavlović, J. Damnjanovic, Z. Girek, Lela Belić, M. Ugrinović","doi":"10.17221/26/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/26/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43762891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gang Jing, Youhua Yao, Li-Gang An, Yongmei Cui, Yixiong Bai, Xin Li, X. Yao, Kunlun Wu
{"title":"Identification and functional analysis of the HvWRKY1 gene associated with Qingke (Hordeum vulgare L. var. nudum Hook. f.) leaf stripe disease","authors":"Gang Jing, Youhua Yao, Li-Gang An, Yongmei Cui, Yixiong Bai, Xin Li, X. Yao, Kunlun Wu","doi":"10.17221/14/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/14/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41747722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiang Su, Kanghua Xian, C. Fu, Jinxiang He, Baojun Liu, N. Huang
{"title":"Selection of suitable reference genes in Paulownia fortunei (Seem.) Hemsl. under different tissues and abiotic stresses for qPCR normalization","authors":"Jiang Su, Kanghua Xian, C. Fu, Jinxiang He, Baojun Liu, N. Huang","doi":"10.17221/72/2022-cjgpb","DOIUrl":"https://doi.org/10.17221/72/2022-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49215310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huali Yang, Wenda Diao, Xiaocui Yan, T. Gebrewahid, Zaifeng Li, Zhanjun Yao
{"title":"Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trials","authors":"Huali Yang, Wenda Diao, Xiaocui Yan, T. Gebrewahid, Zaifeng Li, Zhanjun Yao","doi":"10.17221/9/2023-cjgpb","DOIUrl":"https://doi.org/10.17221/9/2023-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41375529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernand S. Sohindji, Florent J.-B. Quenum, Nicodème V. Fassinou-Hotegni, Adechina Adékounlé Oke, C. Adje, E. Achigan-Dako
{"title":"Crossing possibility for breeding promising orange-fleshed sweetpotato genotypes in Benin","authors":"Fernand S. Sohindji, Florent J.-B. Quenum, Nicodème V. Fassinou-Hotegni, Adechina Adékounlé Oke, C. Adje, E. Achigan-Dako","doi":"10.17221/104/2022-cjgpb","DOIUrl":"https://doi.org/10.17221/104/2022-cjgpb","url":null,"abstract":"","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44561159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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