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Disease resistance of improved MR220 lines against Pyricularia oryzae Cavara and their preliminary agronomic performance MR220改良系对稻瘟病菌的抗病性及其初步农艺性能
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-11-24 DOI: 10.17221/55/2021-cjgpb
Siti Nor Hafiza Mohamad, Mohamad Bahagia Ab Ghaffar, A. S. Othman, S. N. Misman, Zuraida Abd Rahman, Mohd Shahril Firdaus Ab Razak, Z. A. Seman, Muhammad Fairuz Mohd Yusof, Khairulmazmi Ahmad, S. N. Yazid, H. Hashim
Blast disease caused by Pyricularia oryzae is one of the most destructive fungal diseases of rice in Malaysia. Utilisation of resistant varieties is the most efficient management approach towards reducing yield losses. The line IRTP21683 with the Pi9 gene has shown strong resistance against the isolate MPO988.3 of pathotype P0.0, the most prevalent P. oryzae pathotype in Malaysia. Crossing of IRTP21683 was undertaken with the recurrent parent MR220, a susceptible elite Malaysian rice variety, using a marker assisted backcrossing technique with two simple sequence repeat markers, RM19776 and RM7311, as the tag for the Pi9 gene. Twenty BC3F4 lines with the Pi9 gene were resistant when challenged with MPO 988.3. The cluster analysis based on seven agronomic parameters showed that the resistant BC3F4 lines could be divided into four groups, of which the members in group 1 and 2 have shown comparable or better performance than MR220. Five lines in group 1, B220PI9-3-48, B220PI9-3-76, B220PI9-3-77, B220PI9-3-79 and B220PI9-3-82 showed outstanding yield performance with early maturation.
稻瘟病是马来西亚水稻最具破坏性的真菌病害之一。利用抗性品种是减少产量损失的最有效的管理方法。具有Pi9基因的IRTP21683对马来西亚最流行的稻瘟病型P0.0分离株MPO988.3表现出较强的抗性。利用标记辅助回交技术,以两个简单序列重复标记RM19776和RM7311作为Pi9基因的标记,将IRTP21683与马来西亚水稻易感优良品种MR220进行杂交。带有Pi9基因的20个BC3F4细胞系在MPO 988.3攻毒时表现出抗性。基于7个农艺参数的聚类分析表明,BC3F4抗性品系可分为4组,其中1、2组成员表现出与MR220相当或更好的表现。1组5个品系B220PI9-3-48、B220PI9-3-76、B220PI9-3-77、B220PI9-3-79和B220PI9-3-82早熟产量表现突出。
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引用次数: 0
Comparative analysis of late blight resistance R genes and their coding proteins in some potato genotypes 马铃薯抗晚疫病R基因及其编码蛋白的比较分析
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-11-04 DOI: 10.17221/49/2021-cjgpb
H. Mahfouze, O. E. El-Sayed
Late blight (LB) disease can cause potato yield losses in both Egypt and the world. Therefore, the structural analysis of resistance (R) genes responsible for LB resistance will help in understanding their functions. This work aimed to identify the variations between the dominant and recessive alleles of two genes, R3a and R8 at the nucleotide and amino acid levels in five potato genotypes. Two genes of R3a and R8 representing the broad-spectrum LB resistance were amplified by specific primers, which gave one amplicon of 194 and 220 bp of each gene, respectively. Two fragments were sequenced after purification using an ABI 3730xl System DNA Sequencer. The DNA sequence alignments of two genes, R3a and R8, were determined among five selected potato genotypes. The percentage of genetic similarity of the nucleotide sequences of the R3a and R8 genes ranged between (82–83%) and (86–87%), respectively, in comparison to the reference sequences in the nucleotide BLAST. We report on the existence of positional differences in the nucleotide sequences, and base-pair substitutions of two fragments, resulting in amino acid changes between the resistant and susceptible potato genotypes. On the other hand, the highest total number of base-pair substitutions was recorded as 16 in the recessive allele r8 of the varieties Bellini and Cara. However, the lowest number was recorded as four in the dominant allele R3a of the variety Cara. The dendrograms of the five potato genotypes were made up of phylogenetically different clusters, separate from all the other named potato accessions of the two genes. The results of this study will create a solid base for the further understanding of the mechanism of plant-pathogen interactions and supply a theoretical reference for durable resistance to late blight diseases in the potato.
晚疫病(LB)可导致埃及和世界马铃薯产量损失。因此,对LB抗性基因进行结构分析将有助于了解其功能。本研究旨在鉴定5个马铃薯基因型中R3a和R8显性和隐性等位基因在核苷酸和氨基酸水平上的差异。利用特异引物扩增了代表LB广谱抗性的R3a和R8两个基因,分别扩增出194 bp和220 bp的扩增子。纯化后使用ABI 3730xl系统DNA测序仪对两个片段进行测序。测定了5个马铃薯基因型中R3a和R8两个基因的DNA序列比对。R3a和R8基因的核苷酸序列与BLAST中参考序列的遗传相似性百分比分别为(82-83%)和(86-87%)。我们报道了核苷酸序列的位置差异,以及两个片段的碱基对替换,导致抗性和敏感马铃薯基因型之间的氨基酸变化。另一方面,贝利尼和卡拉的隐性等位基因r8的碱基对替换次数最多,为16次。然而,卡拉的显性等位基因R3a的数量最少,为4个。5个马铃薯基因型的树状图由系统发育上不同的簇组成,与这2个基因的所有其他已命名的马铃薯材料分离。本研究结果将为进一步了解植物与病原菌相互作用机理奠定坚实的基础,并为马铃薯抗晚疫病提供理论参考。
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引用次数: 1
A 31-bp indel localised in the 5' untranslated region of OsSUT3 affects the gene expression and rice (Oryza sativa L.) pollen development 位于OsSUT3基因5'非翻译区一个31 bp的缺失影响基因表达和水稻花粉发育
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-11-01 DOI: 10.17221/66/2021-cjgpb
Chunlong Zhang, Qiuping Li, Hong Yang, Tuo Wang, Juan Li, J. Wen, S. Jin, Zhonglin Zhang, Lijuan Chen, Dandan Li
OsSUT genes have been demonstrated to be relevant for diverse biological processes in rice. In this study, we identified the close relationship between a 31-bp insertion in a 5' untranslated region (5' UTR) of the OsSUT3 gene and higher OsSUT3 expression in rice panicles by qRT-PCR and transgenic research. Statistically significant results (P < 0.01) were found for this 31-bp insertions/deletions (indels) in the rice pollen development and other panicle traits, such as the pollen number, pollen fertility, seeding rate, and grain length. An evolution analysis showed that the proportion of the 31-bp insertion significantly increases in rice domestication. Therefore, the 31-bp Indel could be considered as a convenient molecular marker to screen more pollen and better panicle traits in rice breeding.
OsSUT基因已被证明与水稻的各种生物过程有关。在本研究中,我们通过qRT-PCR和转基因研究确定了OsSUT3基因5’非翻译区(5’UTR)中31bp的插入与水稻穗中较高的OsSUT3表达之间的密切关系。这31bp的插入/缺失(indel)对水稻花粉发育和其他穗部性状,如花粉数量、花粉育性、播种率和粒长,都有统计学意义(P<0.01)。进化分析表明,在水稻驯化过程中,31bp插入的比例显著增加。因此,31bp Indel可以作为一种方便的分子标记,在水稻育种中筛选更多的花粉和更好的穗部性状。
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引用次数: 2
Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley 西藏无壳大麦HvnHID基因的分离、测序及其在紫色粒色发育中的作用
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-10-05 DOI: 10.17221/34/2021-cjgpb
X. Yao, Leping Su, Youhua Yao, Li-Gang An, Yixiong Bai, Xin Li, Kunlun Wu
2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of the purple-grained Tibetan hulless barley variety Nerumuzha and the white-grained variety Kunlun 10. The HvnHID gene includes the 981 bp open reading frame and encodes a protein of 327 amino acids. It has a typical Abhydrolase_3 domain (78–306) and belongs to the carboxylesterase (CXE) family of the Abhydrolase_3 (α/β hydrolase) superfamily. There are eight nucleotide differences in the HvnHID coding sequence and two amino acid differences (one in the Abhydrolase_3 domain) between Nerumuzha and Kunlun 10. The HvnHID of hulless barley has the closest relationship with the HID in Hordeum vulgare, and the most distant relationship in Panicum hallii. At the early-mid stage of the seed colour development, the HvnHID expression levels in the purple and black seeds were significantly higher than in the white and blue ones (P < 0.01). During the seed colour development of purple-grained hulless barley, the expression of the key genes (HvnF3'H, HvnDRF, HvnANT1, and HvnGT) in the anthocyanidin biosynthetic pathway increased significantly, while the HvnHID expression decreased significantly (P < 0.01). Thus, it is likely that HvnHID negatively regulates the anthocyanidin biosynthesis. This result provides an important basis for further study of the biological functions of HvnHID in the anthocyanidin biosynthetic pathway.
2-羟基异黄酮脱水酶(HID)在异黄酮的生物合成中起着重要作用。本研究从紫粒西藏无壳大麦品种Nerumuzha和白粒西藏无壳大麦品种昆仑10号的种子中分离出HID。hhnhid基因包括981 bp的开放阅读框,编码327个氨基酸的蛋白质。它具有典型的Abhydrolase_3结构域(78-306),属于Abhydrolase_3 (α/β水解酶)超家族的羧酸酯酶(CXE)家族。在hnnhid编码序列上,Nerumuzha和Kunlun 10存在8个核苷酸差异和2个氨基酸差异(1个在Abhydrolase_3结构域)。无壳大麦的hnhid与普通大麦的亲缘关系最密切,与哈利大麦的亲缘关系最远。在种子颜色发育早期和中期,紫色和黑色种子中hnvhid的表达量极显著高于白色和蓝色种子(P < 0.01)。紫粒无壳大麦种子色发育过程中,花青素生物合成途径关键基因HvnF3'H、HvnDRF、HvnANT1和HvnGT的表达量显著升高,而HvnHID的表达量显著降低(P < 0.01)。因此,hnhid可能负调控花青素的生物合成。该结果为进一步研究hhnhid在花青素生物合成途径中的生物学功能提供了重要依据。
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引用次数: 2
Comparing the number of Iranian pomegranate genotypes based on morphological and biochemical properties 基于形态和生化特性的伊朗石榴基因型数量比较
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-09-14 DOI: 10.17221/39/2021-cjgpb
S. Yari, S. Mirjalili, A. Mousavi, E. Poorazizi
Punica granatum L. is one of the oldest known edible fruits. Numerous chemical compounds have been isolated from pomegranate seeds, juice, and peels, which have beneficial effects on human health. This study aimed to perform the physicochemical and morphological properties of twenty-four pomegranate genotypes from various provinces of Iran. Fifteen fruits of each cultivar are collected at harvest maturity in the normal ripening period for the pomegranate from the Iranian pomegranate genetic collection in Yazd, Iran. Five fruits were randomly harvested from each of four orientations of the tree, and were immediately taken to the laboratory for analysis. Three replicates were maintained for each analysis. The results indicated the highest levels of anthocyanin was observed in S783 and R633, while polyphenols in Q529, the antioxidant capacity in N755 and the total soluble solids levels in R633 and the total acidity levels were found in K477 and E336. On the other hand, the fruit weight (in S948), fruit diameter (in SH1738), crown diameter (in R533), total weight of the seeds (in S948), peel thickness (in S716), peel colour (in S948), and red juice (in S783) are significantly affected by the genotype. At a similarity of 50%, the genotypes were divided into nine sub-clusters including A, B, C, D, E, F, G, H and I. These identified genotypes can be rolled out in future breeding programmes.
石榴是已知最古老的可食用水果之一。从石榴籽、石榴汁和石榴皮中分离出了许多对人体健康有益的化合物。本研究旨在研究来自伊朗不同省份的24个石榴基因型的理化和形态特性。在伊朗亚兹德的伊朗石榴遗传收集中,每个品种的15个果实在石榴的正常成熟期收获成熟时收集。从树的四个方向上随机收获5个果实,并立即被带到实验室进行分析。每次分析维持3个重复。结果表明,花青素含量最高的品种是S783和R633,多酚含量最高的品种是Q529,抗氧化能力最高的品种是N755,总可溶性固形物含量最高的品种是K477和E336。另一方面,果实重量(S948)、果实直径(SH1738)、树冠直径(R533)、种子总重(S948)、果皮厚度(S716)、果皮颜色(S948)和红汁(S783)受基因型的显著影响。在相似度为50%的情况下,将基因型分为9个亚群,包括a、B、C、D、E、F、G、H和i。这些已确定的基因型可以在未来的育种计划中推广。
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引用次数: 2
Development of the new waxy winter wheat cultivars Eldija and Sarta 糯性冬小麦新品种Eldija和Sarta的选育
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-08-17 DOI: 10.17221/37/2021-CJGPB
Ž. Liatukas, V. Ruzgas, A. Gorash, J. Cecevičienė, R. Armonienė, G. Statkevičiūtė, K. Jaškūnė, G. Brazauskas
Two new waxy winter wheat (Triticum aestivum L.) cultivars, Eldija and Sarta, were developed at the Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry and released in Lithuania in 2021. The cultivars were developed using waxy wheat material from Nebraska, the United States of America. The mean yield of Eldija and Sarta at five locations over three testing years was 7.56 and 7.21 t/ha or 79.63 and 75.95%, respectively, compared to the yield of the standard cultivars. Eldija and Sarta should be grown under high input conditions due to the relatively low resistance to leaf spot diseases and Fusarium head blight and medium tolerance to lodging. An amylose content of 0.68% and 0.36% of Eldija and Sarta, respectively, a very low falling number (about 60 s), a lower flour yield and higher water absorption compared to common wheat and the reaction to iodine staining (brown colour) characterised the new cultivars as fully waxy wheats. These cultivars are intended for the potential demand from commercial companies for special use in the food industry.
立陶宛农林研究中心农业研究所开发了两个新的糯性冬小麦品种Eldija和Sarta,并于2021年在立陶宛发布。这些品种是用来自美国内布拉斯加州的糯性小麦材料培育的。与标准品种的产量相比,Eldija和Sarta在三个试验年内在五个地点的平均产量分别为7.56和7.21吨/公顷,即79.63和75.95%。Eldija和Sarta应在高投入条件下种植,因为它们对叶斑病和镰刀菌头枯病的抗性相对较低,对倒伏的耐受性中等。Eldija和Sarta的直链淀粉含量分别为0.68%和0.36%,与普通小麦相比,下降数非常低(约60s),面粉产量较低,吸水率较高,对碘染色的反应(棕色)使新品种成为全糯小麦。这些品种旨在满足商业公司在食品行业的特殊用途的潜在需求。
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引用次数: 3
Association of leaf chlorophyll content with the stay-green trait and grain yield in wheat grown under heat stress conditions 热胁迫条件下小麦叶片叶绿素含量与保绿特性及产量的关系
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-07-16 DOI: 10.17221/45/2021-CJGPB
C. Roy, T. Chattopadhyay, R. Ranjan, Wahid ul Hasan, Abhishek Kumar, Nitish De
Heat stress is a major production constraint of wheat in South Asia, particularly in the Gangetic plains of India and Bangladesh. The leaf chlorophyll status is a key determinant for a high rate of photosynthesis under stress. The present experiments included 238 genotypes in 2016–2017 and 321 genotypes in 2017–2018 under optimum and under heat stress conditions. Subsequently, a set of 100 genotypes selected on basis of the heat susceptibility index was evaluated in 2018–2019 under heat stress conditions to study the relationship between important physiological traits and yield under stress. A significant correlation of soil plant analysis development (SPAD) value of the two upper leaves with stay-green trait and grain yield indicates the importance of chlorophyll content, both in flag and penultimate leaf, in maintaining leaf areas under greenness (LAUG) and grain yield under heat stress. The SPAD in the flag and penultimate leaf was responsible for 8.8% and 10.9%, respectively, of the variation in grain yield. For the stay-green trait, 8.4% and 7.2 % of the variation was governed by the SPAD value in the flag and penultimate leaf, respectively. These results suggest that, in addition to the flag leaf, the chlorophyll status of the penultimate leaf can be an important criterion for the selection of superior wheat genotypes under heat stress. The genotypes SW-139; SW 108; DWR-F8-35-9-1; NHP-F8-130; DWR-F8-3-1 that maintained a high chlorophyll content in the flag and penultimate leaf can be used further in breeding programmes addressing heat resistance in wheat.
热应激是南亚小麦生产的主要制约因素,特别是在印度和孟加拉国的恒河平原。叶片叶绿素状态是胁迫下高光合速率的关键决定因素。本实验在最佳和热胁迫条件下,选取了2016-2017年的238个基因型和2017-2018年的321个基因型。随后,在2018-2019年热胁迫条件下,根据热敏感性指数筛选100个基因型,研究胁迫下重要生理性状与产量的关系。上叶土壤植物分析发育(SPAD)值与保绿性状和籽粒产量呈显著相关,表明旗叶和倒数叶叶绿素含量在热胁迫下维持绿叶面积(LAUG)和籽粒产量方面具有重要意义。旗叶和倒数叶SPAD分别占籽粒产量变异的8.8%和10.9%。在留绿性状中,旗叶和倒数叶的SPAD值分别占变异的8.4%和7.2%。这些结果表明,除旗叶外,倒数第二叶的叶绿素状态可以作为热胁迫条件下小麦优良基因型选择的重要标准。基因型SW-139;SW 108;DWR-F8-35-9-1;额定马力- f8 - 130;在旗叶和倒数叶中保持较高叶绿素含量的DWR-F8-3-1可以进一步用于小麦耐热性育种计划。
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引用次数: 8
Mapping of quantitative trait loci for purple stigma and purple apiculus in rice by using a Zhenshan 97B/Minghui 63 RIL population 水稻紫色柱头和紫色尖数量性状位点的珍山97B/明会63 RIL群体定位
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-07-14 DOI: 10.17221/20/2021-CJGPB
Jiping Tong, Zhengshu Han, A. Han
Anthocyanin pigmentation is an important morphological marker that is commonly used to identify rice varieties and for linkage analysis. The following study investigates the genetic factors involved in the purple stigma (Ps) and purple apiculus (Pa) traits of an important indica rice cross between Zhenshan 97 (purple stigma and purple apiculus) and Minghui 63 (grey stigma and colourless apiculus). A recombinant inbred line (RIL) population derived from this cross was used for quantitative trait loci (QTL) mapping of the purple stigma and purple apiculus traits. As a result, one major QTL for the purple stigma trait, temporarily designated qPS-1-1, and one major QTL for the purple apiculus trait, temporarily designated qPA-1-1, were mapped to the short arm of chromosome 6 in the interval between the two markers Y4073L and *P. The LOD peaks of qPS-1-1 and qPA-1-1 were 44.0127 and 173.3585, respectively. In addition, qPS-1-1 and qPA-1-1 explained 66.7416% and 98.6441% of the total phenotypic variance, respectively. The Zhenshan 97 allele increased the purple stigma trait by approximately 8.0355% (for qPS-1-1) and 9.8863% (for qPA-1-1). Moreover, since qPS-1-1 and qPA-1-1 were strongly correlated, they were also located in the same vicinity of the C gene on the short arm of chromosome 6, which suggested that the two QTL might be the same. By comparing these and previous results, it was deduced that qPS-1-1 or qPA-1-1 was the C gene and was pleiotropic for both the colouration of the apiculus and the colouration of the stigma in rice.
花青素色素沉着是水稻品种鉴定和连锁分析的重要形态标记。本文研究了紫柱头和紫尖的珍汕97与灰柱头和无色尖的明恢63杂交籼稻紫色柱头和紫尖性状的遗传因素。利用该杂交获得的重组自交系(RIL)群体对紫柱头和紫尖性状进行了数量性状位点(QTL)定位。将紫色柱头性状的一个主要QTL(暂称qPS-1-1)和紫色尖性状的一个主要QTL(暂称qPA-1-1)定位在6号染色体短臂Y4073L和*P两个标记之间。qPS-1-1和qPA-1-1的LOD峰值分别为44.0127和173.3585。qPS-1-1和qPA-1-1分别解释了总表型方差的66.7416%和98.6441%。珍山97等位基因使紫色柱头性状分别提高了8.0355% (qPS-1-1)和9.8863% (qPA-1-1)。此外,由于qPS-1-1和qPA-1-1具有强相关性,它们也位于6号染色体短臂上C基因的同一附近,这表明这两个QTL可能是相同的。通过与前人研究结果的比较,推测qPS-1-1或qPA-1-1为C基因,且对水稻尖部和柱头着色均具有多效性。
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引用次数: 2
TPR domain coding gene ST2 may be involved in regulating tillering and fertility in rice TPR结构域编码基因ST2可能参与水稻分蘖和育性调控
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-07-14 DOI: 10.17221/103/2020-CJGPB
G. Wei, Hongxia Yang, Zixian Xiong, Jingwen Wu, Danyang Chen, Yang Liu, Yijie Ban, Weichi Liu, Lina Shang, Nan Wang
A decrease in the tiller number and male sterility will lead to a decline in the rice yield. Therefore, it is significant to study the molecular mechanism of controlling the tiller number and regulating the male reproductive development. The mutant st2 (single tiller 2) was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B and showed single tillering and male sterility. I2-KI staining showed that the st2 pollen was aborted. The scanning electron microscope (SEM) observation underlined that the anther of st2 became smaller, the wax of the epidermis reduced, the inner wall shrank and the Ubisch body decreased, the pollen collapsed, and the germination pore developed abnormally. The genetic analysis discovered that the trait was controlled by a single recessive nuclear gene located on chromosome 3. LOC_Os03g05540 encoding a tetratricopeptide repeat (TPR) domain was identified as the candidate gene by sequencing. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that ST2 was highly expressed in the stem apical meristem (SAM) and the initial stage of meiosis during the anther development. The subcellular localisation indicated that ST2 is a nuclear and plasmic localisation protein. The homology analysis demonstrated that ST2 was evolutionarily conserved. These results laid a foundation for further study of the ST2 function.
分蘖数量和雄性不育性的降低将导致水稻产量的下降。因此,研究控制分蘖数和调节雄性生殖发育的分子机制具有重要意义。用甲烷磺酸乙酯(EMS)诱导籼型保持系西农1B产生突变体st2(单分蘖2),表现为单分蘖和雄性不育。I2-KI染色显示st2花粉脱落。扫描电子显微镜(SEM)观察表明,st2的花药变小,表皮蜡减少,内壁缩小,乌比施体减少,花粉塌陷,萌发孔发育异常。遗传分析发现,该性状由位于3号染色体上的一个隐性核基因控制。通过测序鉴定编码四肽重复序列(TPR)结构域的LOC_Os03g05540为候选基因。定量实时聚合酶链反应(qRT-PCR)分析表明,ST2在花药发育过程中的茎尖分生组织(SAM)和减数分裂初期高度表达。亚细胞定位表明ST2是一种细胞核和细胞质定位蛋白。同源性分析表明ST2在进化上是保守的。这些结果为进一步研究ST2的功能奠定了基础。
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引用次数: 1
Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing 应用全基因组测序技术构建烟草回交BC4F3群体高密度遗传图谱及QTL定位
IF 0.9 4区 农林科学 Q3 AGRONOMY Pub Date : 2021-06-08 DOI: 10.17221/8/2021-CJGPB
Zhijun Tong, Sanjie Jiang, Weiming He, Xue-Jun Chen, Lixin Yin, D. Fang, Yafei Hu, F. Jiao, Chi Zhang, Zeng Jianmin, Xin-yan Wu, Shancen Zhao, Jianbo Jian, B. Xiao
Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.
回交是植物育种的有力工具。改良的标记辅助回交旨在将供体亲本中感兴趣的目标基因或数量性状位点(qtl)转移到复发亲本中。本研究以Y3和K326为杂交亲本,YF1-1为F1亲本,构建烟草BC4F3群体。利用381个家系群体的高通量测序数据,构建了包含24 142个高质量SNP (single nucleotide polymorphism)标记的高密度遗传图谱,平均遗传距离为0.59 cM。然后对所有后代进行基因组模块分析。共鉴定出6个农艺性状的43个候选qtl。该研究为烟草育种提供了原始的生物标记物,并为其他植物回交应用提供了线索。
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引用次数: 1
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Czech Journal of Genetics and Plant Breeding
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