Improving the financial performance of fishery enterprises is an inevitable choice to promote the development of fishery economy. In this paper, 102 observation samples are selected, and the necessary and sufficient conditions for high financial performance of fishery listed companies are analyzed by using collocation perspective and qualitative comparative analysis (QCA). Research has found that the formation of high financial performance is a complex causal relationship with multiple concurrent factors, which is the result of the combined effect of multiple conditions, and the effect of each condition is not equal; Moreover, there is no unique optimal model, and 6 different conditional configurations (paths or models) can achieve the same results. The conclusion of this study is helpful to enriching the research on the factors affecting the financial performance of fishery enterprises from the perspective of system integration, and is of great theoretical and practical significance to reveal the factor combination mode produced by the research tendency of fishery enterprise ’performance.
{"title":"Exploring Multiple Paths to Improve Fishery Financial Performance from a Configuration Perspective: A Study Based on the fsQCA Method","authors":"Siyuan Zhang, Shiwei Xu, Xuping Huang, Yiqi Wang","doi":"10.46989/001c.122113","DOIUrl":"https://doi.org/10.46989/001c.122113","url":null,"abstract":"Improving the financial performance of fishery enterprises is an inevitable choice to promote the development of fishery economy. In this paper, 102 observation samples are selected, and the necessary and sufficient conditions for high financial performance of fishery listed companies are analyzed by using collocation perspective and qualitative comparative analysis (QCA). Research has found that the formation of high financial performance is a complex causal relationship with multiple concurrent factors, which is the result of the combined effect of multiple conditions, and the effect of each condition is not equal; Moreover, there is no unique optimal model, and 6 different conditional configurations (paths or models) can achieve the same results. The conclusion of this study is helpful to enriching the research on the factors affecting the financial performance of fishery enterprises from the perspective of system integration, and is of great theoretical and practical significance to reveal the factor combination mode produced by the research tendency of fishery enterprise ’performance.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"9 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141928820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuanyuan Zhang, Hong Lu, Han Ke, Hui-zhong Cheng, Yong-an Zhu, Li-ping Song, Hong-yan Tian, Wen-wen Huang
This experiment aimed to investigate the effects of emodin on the physiological responses and antioxidant gene expression of Wuchang bream infected with Aeromonas hydrophila. The experimental diets were prepared with supplementing 0, 30, 100 and 150 mg kg−1 emodin to basal (control) diet respectively, and fed to fish with initial weight of 50.4 ± 2.35 g. All fish were divided into five experimental groups: uninfected fish fed with basal control diet (negative control, NC), infected fish fed with the diet supplemented with 0 (positive control group, PC), 30, 100, and 150 mg kg-1 emodin. The fish were reared for 14 days, sampled at different time points and then analyzed. The results showed that the physiological responses and related antioxidant gene expression of infected Wuchang bream were significantly influenced by the dosage of added emodin and the feeding duration (P < 0.05). Comparing to the positive control group, emodin could inhibit the levels of cortisol (COR), triiodothyronine (T3), and thyroxine (T4) in infected Wuchang bream, with hormone levels reaching equilibrium in the shortest time at 30 and 100 mg kg-1 emodin supplementation. Meanwhile, emodin significantly affected alkaline phosphatase (AKP) activity, glucose (Glu) and triglyceride (TG) contents, and related antioxidant gene expression in infected Wuchang bream (P < 0.05), with the best effect observed at 100 mg kg-1 emodin supplementation in the diet. In conclusion, the supplementation of 100 mg/kg emodin to diet can enhance the resistance of Wuchang bream to A. hydrophila infection via promoting physiological metabolism and antioxidant capacity.
{"title":"Effects of emodin on the physiological responses and antioxidant gene expression of Wuchang bream infected with Aeromonas hydrophila","authors":"Yuanyuan Zhang, Hong Lu, Han Ke, Hui-zhong Cheng, Yong-an Zhu, Li-ping Song, Hong-yan Tian, Wen-wen Huang","doi":"10.46989/001c.120385","DOIUrl":"https://doi.org/10.46989/001c.120385","url":null,"abstract":"This experiment aimed to investigate the effects of emodin on the physiological responses and antioxidant gene expression of Wuchang bream infected with Aeromonas hydrophila. The experimental diets were prepared with supplementing 0, 30, 100 and 150 mg kg−1 emodin to basal (control) diet respectively, and fed to fish with initial weight of 50.4 ± 2.35 g. All fish were divided into five experimental groups: uninfected fish fed with basal control diet (negative control, NC), infected fish fed with the diet supplemented with 0 (positive control group, PC), 30, 100, and 150 mg kg-1 emodin. The fish were reared for 14 days, sampled at different time points and then analyzed. The results showed that the physiological responses and related antioxidant gene expression of infected Wuchang bream were significantly influenced by the dosage of added emodin and the feeding duration (P < 0.05). Comparing to the positive control group, emodin could inhibit the levels of cortisol (COR), triiodothyronine (T3), and thyroxine (T4) in infected Wuchang bream, with hormone levels reaching equilibrium in the shortest time at 30 and 100 mg kg-1 emodin supplementation. Meanwhile, emodin significantly affected alkaline phosphatase (AKP) activity, glucose (Glu) and triglyceride (TG) contents, and related antioxidant gene expression in infected Wuchang bream (P < 0.05), with the best effect observed at 100 mg kg-1 emodin supplementation in the diet. In conclusion, the supplementation of 100 mg/kg emodin to diet can enhance the resistance of Wuchang bream to A. hydrophila infection via promoting physiological metabolism and antioxidant capacity.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"98 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141663957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aquatic products have played an increasingly important role in residents’ diets, with improved production capacity and living standards in recent years. Accelerating the organic diffusion of aquaculture technology is an effective way to increase production. Taking the example of South American white shrimp aquaculture, this article combines carbon emissions and aquaculture cost benefits. By using data from South American white shrimp aquaculture in Hebei, Shandong, and Jiangsu Province in China from 2016 to 2021, the article innovatively considers aquaculture cycle carbon emissions as non-expected output, constructs a non-expected SBM-DEA super-efficiency model to evaluate the comprehensive efficiency of two aquaculture technologies, and analyzes the influencing factors of the diffusion of shrimp culture technology through case studies. The research results show that the comprehensive efficiency of factory-based aquaculture technology is generally better than pond-based aquaculture, but carbon emissions are higher in the factory model. The main factors affecting the diffusion of factory-based technology are policy support, social networks, farmers’ own situations, and technological attributes.
{"title":"The diffusion path and influencing factors of shrimp farming technology","authors":"Hongtao Jin, Lele Xiang, Fang Chen, Wenjun Zhu","doi":"10.46989/001c.120489","DOIUrl":"https://doi.org/10.46989/001c.120489","url":null,"abstract":"Aquatic products have played an increasingly important role in residents’ diets, with improved production capacity and living standards in recent years. Accelerating the organic diffusion of aquaculture technology is an effective way to increase production. Taking the example of South American white shrimp aquaculture, this article combines carbon emissions and aquaculture cost benefits. By using data from South American white shrimp aquaculture in Hebei, Shandong, and Jiangsu Province in China from 2016 to 2021, the article innovatively considers aquaculture cycle carbon emissions as non-expected output, constructs a non-expected SBM-DEA super-efficiency model to evaluate the comprehensive efficiency of two aquaculture technologies, and analyzes the influencing factors of the diffusion of shrimp culture technology through case studies. The research results show that the comprehensive efficiency of factory-based aquaculture technology is generally better than pond-based aquaculture, but carbon emissions are higher in the factory model. The main factors affecting the diffusion of factory-based technology are policy support, social networks, farmers’ own situations, and technological attributes.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":" 98","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141671030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
espl1 (extra spindle pole bodies like 1), a cysteine endopeptidase, is a mitotic key player in chromosomal segregation and centriole duplication during mitosis and meiosis. Considering the espl1 gene has not been reported in aquatic organisms, we reported the isolation and expression of the espl1 gene from loach. In this study, the full-length cDNA of espl1 was cloned for the first time in loach. In loach, the full-length cDNA of espl1 consisted of 6948 bp, the open reading frame (ORF) is 6240 bp, and the espl1 gene encodes 2139 amino acids. Moreover, the deduced amino acid sequences of espl1 in loach shared the highest identity with those of Cyprinus carpio (78.39%) and Sinocyclocheilus anshuiensis (78.27%), and the sequence homology among the various separases is confined to the C-terminal region. Furthermore, tissue-specific checking results indicated that the espl1 gene of the loach gene was highly expressed in the ovary and testis, especially in stage Ⅳ oocytes and stage Ⅳ testis by Real-time quantitative PCR (qPCR). Then, whole-mount in situ hybridization analyses revealed the expression of espl1 in the early development of loach. We found that the positive signal of espl1 was observed in the notochord during the early embryo development of loach. Last but not least, when treated with luteinizing hormone-releasing hormone analog (LHRH-A2), the mRNA expression of espl1 was significantly increased in the testes and ovaries. These observations suggest that the espl1 gene had a distinct and important role in the gonads of Loach. This study will be of value for further studies into the function of the espl1 gene in fish.
espl1（extra spindle pole bodies like 1）是一种半胱氨酸内肽酶，是有丝分裂和减数分裂过程中染色体分离和中心粒复制的关键角色。考虑到espl1基因在水生生物中尚未见报道，我们报道了泥鳅中espl1基因的分离和表达。本研究首次在泥鳅中克隆了espl1的全长cDNA。在泥鳅中，espl1的全长cDNA为6948 bp，开放阅读框（ORF）为6240 bp，espl1基因编码2139个氨基酸。此外，泥鳅中espl1的氨基酸序列推导结果与鲤鱼（78.39%）和安溪鳜鱼（78.27%）的氨基酸序列相同度最高，且各种分离酶之间的同源性仅限于C端区域。此外，组织特异性检测结果表明，泥鳅espl1基因在卵巢和睾丸中高表达，尤其是在Ⅳ期卵母细胞和Ⅳ期睾丸中。然后，通过全图原位杂交分析发现了 espl1 在泥鳅早期发育中的表达。我们发现，在泥鳅早期胚胎发育过程中，espl1的阳性信号出现在脊索上。最后，当使用黄体生成素释放激素类似物（LHRH-A2）处理泥鳅时，espl1在睾丸和卵巢中的mRNA表达量显著增加。这些观察结果表明，espl1基因在泥鳅性腺中具有独特而重要的作用。这项研究对进一步研究 espl1 基因在鱼类中的功能具有重要价值。
{"title":"Cloning and expression patterns of espl1 gene in loach (Misgurnus anguillicaudatus) during gonad development","authors":"Hanjun Jiang, Qianqian Huang, Xusheng Guo, Jiahui Liu, Dexiang Feng, Xiaojuan Cao","doi":"10.46989/001c.116379","DOIUrl":"https://doi.org/10.46989/001c.116379","url":null,"abstract":"espl1 (extra spindle pole bodies like 1), a cysteine endopeptidase, is a mitotic key player in chromosomal segregation and centriole duplication during mitosis and meiosis. Considering the espl1 gene has not been reported in aquatic organisms, we reported the isolation and expression of the espl1 gene from loach. In this study, the full-length cDNA of espl1 was cloned for the first time in loach. In loach, the full-length cDNA of espl1 consisted of 6948 bp, the open reading frame (ORF) is 6240 bp, and the espl1 gene encodes 2139 amino acids. Moreover, the deduced amino acid sequences of espl1 in loach shared the highest identity with those of Cyprinus carpio (78.39%) and Sinocyclocheilus anshuiensis (78.27%), and the sequence homology among the various separases is confined to the C-terminal region. Furthermore, tissue-specific checking results indicated that the espl1 gene of the loach gene was highly expressed in the ovary and testis, especially in stage Ⅳ oocytes and stage Ⅳ testis by Real-time quantitative PCR (qPCR). Then, whole-mount in situ hybridization analyses revealed the expression of espl1 in the early development of loach. We found that the positive signal of espl1 was observed in the notochord during the early embryo development of loach. Last but not least, when treated with luteinizing hormone-releasing hormone analog (LHRH-A2), the mRNA expression of espl1 was significantly increased in the testes and ovaries. These observations suggest that the espl1 gene had a distinct and important role in the gonads of Loach. This study will be of value for further studies into the function of the espl1 gene in fish.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":" 71","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141672852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Xu, Yang Zhou, Weijie Cui, Qun Lu, Jianhu Liu, Cong Duan, Tao He
The Spawning ground is a crucial habitat for fish, and the physical characteristics of the habitat are important for fish life history. In this paper, Large-Scale Particle Image Velocimetry (LSPIV) was used to measure the surface flow field and the substrate (particle size and mineral composition) in the spawning ground of Schizothorax grahami. Based on the literature and consideration of experimental feasibility, two spawning grounds in the source of the Chishui River were selected—Erlongqiangbao (located in the mainstream) and Bamaoba (located in the tributary), two and three cross sections of each river section were analyzed respectively. The results show that Erlongqiangbao first flows in the direction of “southeast-northwest” and then “southwest—northeast” after crossing the bridge; the maximum velocity of the reach is 3.374m/s. The size of the substrate is mainly less than 1mm in the upstream section of Erlongqiangbao, both 25-50mm in the middle and downstream, and the substrate type mainly is quartz and calcite; Gushangtian flows in the direction of “southwest-northeast,” the maximum velocity of the reach is 1.537m/s, the size of the substrate is mainly greater than 50mm in the upstream, and 25-50mm in the middle and downstream, the substrate type mainly is quartz, calcite, and dolomite. The mineral compositions of the two spawning sites were analyzed by principal component analysis. Quartz and anorthose are more than 1 in composite scores, respectively ranked first and second, indicating that they play a dominant role in the mineral composition. This study could provide basic data and ideas for restoring S. grahami spawning ground.
{"title":"Characteristics of surface flow field and substrate in the spawning ground of Schizothorax grahami","authors":"Li Xu, Yang Zhou, Weijie Cui, Qun Lu, Jianhu Liu, Cong Duan, Tao He","doi":"10.46989/001c.120233","DOIUrl":"https://doi.org/10.46989/001c.120233","url":null,"abstract":"The Spawning ground is a crucial habitat for fish, and the physical characteristics of the habitat are important for fish life history. In this paper, Large-Scale Particle Image Velocimetry (LSPIV) was used to measure the surface flow field and the substrate (particle size and mineral composition) in the spawning ground of Schizothorax grahami. Based on the literature and consideration of experimental feasibility, two spawning grounds in the source of the Chishui River were selected—Erlongqiangbao (located in the mainstream) and Bamaoba (located in the tributary), two and three cross sections of each river section were analyzed respectively. The results show that Erlongqiangbao first flows in the direction of “southeast-northwest” and then “southwest—northeast” after crossing the bridge; the maximum velocity of the reach is 3.374m/s. The size of the substrate is mainly less than 1mm in the upstream section of Erlongqiangbao, both 25-50mm in the middle and downstream, and the substrate type mainly is quartz and calcite; Gushangtian flows in the direction of “southwest-northeast,” the maximum velocity of the reach is 1.537m/s, the size of the substrate is mainly greater than 50mm in the upstream, and 25-50mm in the middle and downstream, the substrate type mainly is quartz, calcite, and dolomite. The mineral compositions of the two spawning sites were analyzed by principal component analysis. Quartz and anorthose are more than 1 in composite scores, respectively ranked first and second, indicating that they play a dominant role in the mineral composition. This study could provide basic data and ideas for restoring S. grahami spawning ground.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"19 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141685029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we investigated the effects of predation by the whelk Neptunea arthritica cumingii on the culture and survival of scallops (Mizuhopecten yessoensis) during bottom culture. The behavioral characteristics of three sizes (small, medium, and large) of scallops were assessed in response to exposure to N. cumingii. We also measured the activities of superoxide dismutase, catalase, arginine kinase, and octopine dehydrogenase in the gill, adductor muscle, and mantle tissues of scallops before and after exposure to predation. We found that scallops escaped from N. cumingii predation by continuous shell closure or movement. The shell closure force increased with the scallop size, and scallops of the same size that were stimulated by the presence of N. cumingii closed their shells more frequently than control scallops. The shell closure ability of scallops of all sizes decreased as the duration of the experiment continued. The enzyme activities in the three scallop tissues changed significantly after continuous stimulation, and the trend was particularly obvious for the enzyme activities in the adductor muscles of medium-sized scallops. Transcriptome analysis of the adductor muscles from medium-sized scallops detected 405 differentially expressed genes (172 upregulated and 233 downregulated), where the expression levels of RYK, APC, BAMBI, WNT16, CACYBP, and RUVBL1 changed after stimulation by N. cumingii. The sequencing results were verified by quantitative real-time PCR. We hypothesize these genes may be related to muscle movement and regulation in M. yessoensis after stimulation.
在这项研究中，我们调查了在底层养殖过程中，海螺Neptunea arthritica cumingii的捕食对扇贝（Mizuhopecten yessoensis）的养殖和存活的影响。我们评估了三种规格（小、中、大）扇贝的行为特征对暴露于 N. cumingii 的反应。我们还测量了扇贝在暴露于捕食前后鳃、内收肌和套膜组织中超氧化物歧化酶、过氧化氢酶、精氨酸激酶和章鱼碱脱氢酶的活性。我们发现，扇贝通过持续闭壳或移动来躲避 N. cumingii 的捕食。扇贝的闭壳力随扇贝的大小而增加，相同大小的扇贝在N. cumingii的刺激下闭壳的频率高于对照组扇贝。随着实验时间的延长，各种大小扇贝的闭壳能力都有所下降。三种扇贝组织的酶活性在持续刺激后发生了显著变化，中型扇贝内收肌的酶活性变化趋势尤为明显。对中型扇贝内收肌的转录组分析发现了405个差异表达基因（172个上调，233个下调），其中RYK、APC、BAMBI、WNT16、CACYBP和RUVBL1的表达水平在受到N.实时定量 PCR 验证了测序结果。我们推测这些基因可能与 M. yessoensis 受刺激后的肌肉运动和调节有关。
{"title":"Behavioral characteristics and transcriptome analysis of Mizuhopecten yessoensis in response to Neptunea arthritica cumingii predation during laboratory bottom-sowing culture","authors":"Yang Liu, Danyang Li, Ying Tian, Junxia Mao, Xubo Wang, Zhenlin Hao","doi":"10.46989/001c.117407","DOIUrl":"https://doi.org/10.46989/001c.117407","url":null,"abstract":"In this study, we investigated the effects of predation by the whelk Neptunea arthritica cumingii on the culture and survival of scallops (Mizuhopecten yessoensis) during bottom culture. The behavioral characteristics of three sizes (small, medium, and large) of scallops were assessed in response to exposure to N. cumingii. We also measured the activities of superoxide dismutase, catalase, arginine kinase, and octopine dehydrogenase in the gill, adductor muscle, and mantle tissues of scallops before and after exposure to predation. We found that scallops escaped from N. cumingii predation by continuous shell closure or movement. The shell closure force increased with the scallop size, and scallops of the same size that were stimulated by the presence of N. cumingii closed their shells more frequently than control scallops. The shell closure ability of scallops of all sizes decreased as the duration of the experiment continued. The enzyme activities in the three scallop tissues changed significantly after continuous stimulation, and the trend was particularly obvious for the enzyme activities in the adductor muscles of medium-sized scallops. Transcriptome analysis of the adductor muscles from medium-sized scallops detected 405 differentially expressed genes (172 upregulated and 233 downregulated), where the expression levels of RYK, APC, BAMBI, WNT16, CACYBP, and RUVBL1 changed after stimulation by N. cumingii. The sequencing results were verified by quantitative real-time PCR. We hypothesize these genes may be related to muscle movement and regulation in M. yessoensis after stimulation.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"17 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141342064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thi Cam Tu Phan, Thi Thu Nguyen, Thi Kim Lien Nguyen, Truong Giang Huynh, Thi Thanh Hien Tran
Probiotics are widely applied in aquaculture, which can improve the growth, survival and health of aquatic animals. The aim of this study was to isolate and evaluate a Lactobacillus strain from the intestine of healthy whiteleg shrimp, Litopenaeus vannamei. A total of 20 Lactobacillus isolates showed rod-shaped morphology, gram-positive, non-motile, non-spore forming, and catalase and oxidase negative. Among the isolates, strain CMT1 exhibited the highest antibacterial activity against Vibrio parahaemolyticus and extracellular enzyme activity, which was selected and identified as Lactobacillus plantarum CMT1 based on 16S rDNA gene sequencing. This strain was also chosen to evaluate its effects on growth and survival of the whiteleg shrimp as a feed supplement. Four different diets were prepared, including the control (a commercial diet without L. plantarum CMT1) and three different concentrations of L. plantarum CMT1, viz. T1 (106 CFU kg diet-1), T2 (107 CFU kg diet-1), and T3 (108 CFU kg diet-1). After a 56-day feeding trial, the growth parameters in the T3 group had the highest values and showed a significant difference compared to the other groups. The survival rates of shrimp fed T2 and T3 diets were significantly higher than those in the control diet (p < 0.05). The lowest FCR value was recorded in the T3 group and showed a significant difference compared to the control group (p < 0.05). In addition, Lactobacillus counts in T2 and T3 groups increased significantly from day 28, which indicated that L. plantarum CMT1 could sustain its population in the intestine of shrimp. However, the total Vibrio sp. count in the T2 and T3 groups was significantly lower than that in the control group (p < 0.05). In conclusion, L. plantarum CMT1 could be used as a potential probiotic for shrimp aquaculture, and the recommended level of the probiotic strain CMT1 is 108 CFU kg diet-1.
益生菌被广泛应用于水产养殖业，可改善水产动物的生长、存活和健康。本研究旨在从健康的南美白对虾肠道中分离和评估乳酸杆菌菌株。共分离出 20 株乳酸杆菌，其形态呈杆状，革兰氏阳性，无运动性，不形成孢子，过氧化氢酶和氧化酶阴性。在分离菌株中，菌株 CMT1 对副溶血性弧菌的抗菌活性最高，胞外酶活性也最高，根据 16S rDNA 基因测序，该菌株被选中并鉴定为植物乳杆菌 CMT1。选择该菌株的目的还在于评估其作为饲料添加剂对南美白对虾生长和存活的影响。制备了四种不同的日粮，包括对照组（不含植物乳杆菌 CMT1 的商品日粮）和三种不同浓度的植物乳杆菌 CMT1，即 T1（106 CFU kg 日粮-1）、T2（107 CFU kg 日粮-1）和 T3（108 CFU kg 日粮-1）。经过 56 天的喂养试验，T3 组的生长参数值最高，与其他组相比差异显著。投喂 T2 和 T3 日粮的对虾存活率明显高于对照组（p < 0.05）。T3 组的 FCR 值最低，与对照组相比差异显著（p < 0.05）。此外，从第 28 天开始，T2 和 T3 组的乳酸杆菌数量明显增加，这表明植物乳杆菌 CMT1 可维持其在对虾肠道中的数量。然而，T2 和 T3 组的弧菌总数明显低于对照组（p < 0.05）。总之，植物弧菌 CMT1 可用作对虾养殖的潜在益生菌，益生菌株 CMT1 的推荐用量为 108 CFU kg diet-1。
{"title":"Isolation of Lactobacillus plantarum CMT1 from shrimp intestine and its effects on growth and survival of the whiteleg shrimp, Litopeneaus vannamei","authors":"Thi Cam Tu Phan, Thi Thu Nguyen, Thi Kim Lien Nguyen, Truong Giang Huynh, Thi Thanh Hien Tran","doi":"10.46989/001c.117555","DOIUrl":"https://doi.org/10.46989/001c.117555","url":null,"abstract":"Probiotics are widely applied in aquaculture, which can improve the growth, survival and health of aquatic animals. The aim of this study was to isolate and evaluate a Lactobacillus strain from the intestine of healthy whiteleg shrimp, Litopenaeus vannamei. A total of 20 Lactobacillus isolates showed rod-shaped morphology, gram-positive, non-motile, non-spore forming, and catalase and oxidase negative. Among the isolates, strain CMT1 exhibited the highest antibacterial activity against Vibrio parahaemolyticus and extracellular enzyme activity, which was selected and identified as Lactobacillus plantarum CMT1 based on 16S rDNA gene sequencing. This strain was also chosen to evaluate its effects on growth and survival of the whiteleg shrimp as a feed supplement. Four different diets were prepared, including the control (a commercial diet without L. plantarum CMT1) and three different concentrations of L. plantarum CMT1, viz. T1 (106 CFU kg diet-1), T2 (107 CFU kg diet-1), and T3 (108 CFU kg diet-1). After a 56-day feeding trial, the growth parameters in the T3 group had the highest values and showed a significant difference compared to the other groups. The survival rates of shrimp fed T2 and T3 diets were significantly higher than those in the control diet (p < 0.05). The lowest FCR value was recorded in the T3 group and showed a significant difference compared to the control group (p < 0.05). In addition, Lactobacillus counts in T2 and T3 groups increased significantly from day 28, which indicated that L. plantarum CMT1 could sustain its population in the intestine of shrimp. However, the total Vibrio sp. count in the T2 and T3 groups was significantly lower than that in the control group (p < 0.05). In conclusion, L. plantarum CMT1 could be used as a potential probiotic for shrimp aquaculture, and the recommended level of the probiotic strain CMT1 is 108 CFU kg diet-1.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"74 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141338195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Choresca, Princess P. Taberna, Fiona L. Pedroso, Faith Loraine T. Magbanua, M. T. T. Oclos, M. J. C. Danting
The climbing perch (Anabas testudineus) is a valuable fish species with significant potential for aquaculture. However, a low survival rate was observed at its early development stages, requiring an optimized protocol for sustainable aquaculture production. This paper presents an improved breeding and seed production technology for climbing perch. Mature climbing perch were induced to spawn using salmon gonadotropin-releasing hormone analog (sGnRHa) at a dose of 30 µg/kg-1 body weight of the breeder. The female broodfish spawn 9–10 hours after hormone injection. Developmental stages were defined based on the morphological features of the embryos from the first cleavage to the hatching stage. The first cleavage began at approximately 18–20 minutes after fertilization. Newly hatched larvae were observed within 18–20 h at 30°C, having an average larval size of 0.389±0.042 mm (total length). Successful breeding entails a selection of suitable algal species, an optimal aquaculture environment, regular feeding rates, consistent monitoring of larval development, and effective management of water quality. Survival rates from the larval stage to fry were achieved at 84.69% and 77.60%, respectively, while the survival rate to the fingerlings stage was 72.51%. Therefore, by implementing an optimized protocol, aquaculture practitioners can maximize the production potential of climbing perch while ensuring sustainable cultivation practices. The findings from this research contribute to the advancement of climbing perch aquaculture by providing valuable insights for the successful cultivation and increased economic profitability of A. testudineus species in hatchery productions.
{"title":"Improved breeding and seed production of climbing perch (Anabas testudineus) in controlled tanks and cage systems","authors":"C. Choresca, Princess P. Taberna, Fiona L. Pedroso, Faith Loraine T. Magbanua, M. T. T. Oclos, M. J. C. Danting","doi":"10.46989/001c.116472","DOIUrl":"https://doi.org/10.46989/001c.116472","url":null,"abstract":"The climbing perch (Anabas testudineus) is a valuable fish species with significant potential for aquaculture. However, a low survival rate was observed at its early development stages, requiring an optimized protocol for sustainable aquaculture production. This paper presents an improved breeding and seed production technology for climbing perch. Mature climbing perch were induced to spawn using salmon gonadotropin-releasing hormone analog (sGnRHa) at a dose of 30 µg/kg-1 body weight of the breeder. The female broodfish spawn 9–10 hours after hormone injection. Developmental stages were defined based on the morphological features of the embryos from the first cleavage to the hatching stage. The first cleavage began at approximately 18–20 minutes after fertilization. Newly hatched larvae were observed within 18–20 h at 30°C, having an average larval size of 0.389±0.042 mm (total length). Successful breeding entails a selection of suitable algal species, an optimal aquaculture environment, regular feeding rates, consistent monitoring of larval development, and effective management of water quality. Survival rates from the larval stage to fry were achieved at 84.69% and 77.60%, respectively, while the survival rate to the fingerlings stage was 72.51%. Therefore, by implementing an optimized protocol, aquaculture practitioners can maximize the production potential of climbing perch while ensuring sustainable cultivation practices. The findings from this research contribute to the advancement of climbing perch aquaculture by providing valuable insights for the successful cultivation and increased economic profitability of A. testudineus species in hatchery productions.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"31 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141103888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Xuan Huy, Nguyen Ty, Tran Van Giang, Tran Vinh Phuong
This study investigates the genetic diversity and population structure of the greasyback shrimp, Metapenaeus ensis (De Haan, 1844), within the Tam Giang – Cau Hai lagoon, Vietnam, by analyzing mtCOI genes from 91 individuals collected across four populations in nine sampling locations. High genetic diversity was found, with 34 unique haplotypes and 38 genetic variations identified. Most genetic variation occurred within populations (AMOVA), suggesting high gene flow. Low and non-significant Fst values and close genetic distances confirmed minimal differentiation among populations. These findings provide the first insights into M. ensis population genetics in this lagoon, informing conservation and management efforts. Understanding genetic diversity is crucial for sustainable management and conservation of marine species. This research aids in understanding population resilience, potential impacts of overfishing, and may assist in developing sustainable harvest strategies of this economically important shrimp species.
本研究通过分析在 9 个采样点的 4 个种群中收集的 91 个个体的 mtCOI 基因，调查了越南 Tam Giang - Cau Hai 泻湖中油背对虾（Metapenaeus ensis，De Haan，1844 年）的遗传多样性和种群结构。结果发现遗传多样性很高，有 34 个独特的单倍型和 38 个遗传变异。大多数遗传变异发生在种群内部（AMOVA），表明基因流动很频繁。较低且不显著的 Fst 值和较近的遗传距离证实了种群间的差异极小。这些发现首次揭示了该环礁湖中 M. ensis 的种群遗传学，为保护和管理工作提供了信息。了解遗传多样性对于海洋物种的可持续管理和保护至关重要。这项研究有助于了解种群的恢复能力、过度捕捞的潜在影响，并有助于制定这一具有重要经济价值的虾类物种的可持续捕捞战略。
{"title":"A First Look at Genetic Diversity of Metapenaeus ensis Populations in Tam Giang – Cau Hai Lagoon, Vietnam","authors":"Nguyen Xuan Huy, Nguyen Ty, Tran Van Giang, Tran Vinh Phuong","doi":"10.46989/001c.117579","DOIUrl":"https://doi.org/10.46989/001c.117579","url":null,"abstract":"This study investigates the genetic diversity and population structure of the greasyback shrimp, Metapenaeus ensis (De Haan, 1844), within the Tam Giang – Cau Hai lagoon, Vietnam, by analyzing mtCOI genes from 91 individuals collected across four populations in nine sampling locations. High genetic diversity was found, with 34 unique haplotypes and 38 genetic variations identified. Most genetic variation occurred within populations (AMOVA), suggesting high gene flow. Low and non-significant Fst values and close genetic distances confirmed minimal differentiation among populations. These findings provide the first insights into M. ensis population genetics in this lagoon, informing conservation and management efforts. Understanding genetic diversity is crucial for sustainable management and conservation of marine species. This research aids in understanding population resilience, potential impacts of overfishing, and may assist in developing sustainable harvest strategies of this economically important shrimp species.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"14 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140963015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. C. Thien, Ho T. Manh, Duong T. Quyen, Nguyen T.L. Xuan, Nguyen T. Lan, Nguyen M. Hung
Snails are the first intermediate host in the life cycle of trematodes. Previous research found that Trichopodus microlepis fish, cultured in earthen ponds in Tan Nhut Commune, Binh Chanh District, Ho Chi Minh City, Vietnam had a high infection rate with metacercariae of Centrocestus formosanus and Procevorum sp. It is necessary to investigate whether habitats supplying water to the ponds may contribute to the transmission by carrying intermediate hosts and/or cercariae into these ponds or not. A study of cercariae and snail composition in rice fields and canal supplying water directly to these Trichopodus microlepis ponds was carried out with six samplings in the years 2022 and 2023. A total of 1,151 snails were collected, and nine snail species belonging to 8 genera and 6 families were identified. Three snail species in both rice fields and canal were infected with trematode (cercariae stage) including Bithynia siamensis, Lymnaea viridis and Indoplanorbis exustus. The overall prevalence in the dry season (25.9%) was significantly higher than in the wet season (10.6%) (P<0.05). Three cercariae morphotypes were recovered from snails: xiphidio (in all three snails), echinostome (in Lymnaea viridis and Indoplanorbis exustus), and pleurolophocercous in Bithynia siamensis. This finding shows that Bithynia siamensis is one of the infected sources for Trichopodus microlepis fish cultured in ponds, as pleurolophocercous is also the cercariae in the family of Heterophyidae (including Centrocestus formosanus and Procevorum sp.). Further research should be done to clarify the transmission of fish-borne zoonotic diseases to cultured Trichopodus microlepis in other habitats including the actual fishponds, and sources of trematodes in order to produce Trichopodus microlepis free of metacercariae, contributing to clean aquaculture development and safe food for local people in the area.
{"title":"Cercariae from snails in rice fields and canal supplying water directly to Trichopodus microlepis fish cultured in earthen ponds in Binh Chanh district, Ho Chi Minh City, Vietnam.","authors":"P. C. Thien, Ho T. Manh, Duong T. Quyen, Nguyen T.L. Xuan, Nguyen T. Lan, Nguyen M. Hung","doi":"10.46989/001c.117344","DOIUrl":"https://doi.org/10.46989/001c.117344","url":null,"abstract":"Snails are the first intermediate host in the life cycle of trematodes. Previous research found that Trichopodus microlepis fish, cultured in earthen ponds in Tan Nhut Commune, Binh Chanh District, Ho Chi Minh City, Vietnam had a high infection rate with metacercariae of Centrocestus formosanus and Procevorum sp. It is necessary to investigate whether habitats supplying water to the ponds may contribute to the transmission by carrying intermediate hosts and/or cercariae into these ponds or not. A study of cercariae and snail composition in rice fields and canal supplying water directly to these Trichopodus microlepis ponds was carried out with six samplings in the years 2022 and 2023. A total of 1,151 snails were collected, and nine snail species belonging to 8 genera and 6 families were identified. Three snail species in both rice fields and canal were infected with trematode (cercariae stage) including Bithynia siamensis, Lymnaea viridis and Indoplanorbis exustus. The overall prevalence in the dry season (25.9%) was significantly higher than in the wet season (10.6%) (P<0.05). Three cercariae morphotypes were recovered from snails: xiphidio (in all three snails), echinostome (in Lymnaea viridis and Indoplanorbis exustus), and pleurolophocercous in Bithynia siamensis. This finding shows that Bithynia siamensis is one of the infected sources for Trichopodus microlepis fish cultured in ponds, as pleurolophocercous is also the cercariae in the family of Heterophyidae (including Centrocestus formosanus and Procevorum sp.). Further research should be done to clarify the transmission of fish-borne zoonotic diseases to cultured Trichopodus microlepis in other habitats including the actual fishponds, and sources of trematodes in order to produce Trichopodus microlepis free of metacercariae, contributing to clean aquaculture development and safe food for local people in the area.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"40 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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