Peican Zhu, Fukai Wang, Biao Wu, Feng Wang, Chunde Wang, Xiaotong Zhang, Kai Yu, Bo Liu
An ecologically and economically important species in East Asia, the natural resources of pen shell Atrina pectinate have suffered severe population declines due to habitat destruction, pollution, and overfishing. Assessing genetic diversity and population structure is the basis for establishing conservation programs for A. pectinate. Our data indicated that high genetic diversity was found in all six populations, with mean allelic richness (Ar) ranging from 8.255 to 9.734, mean observed heterozygosity (Ho) from 0.574 to 0.680, and mean expected heterozygosity (He) from 0.620 to 0.691. The five A. pectinate populations were divided into two clusters. This clustering result was partly consistent with their geographical origin; the RZ population did not cluster with the northern populations (DL, CD), suggesting that there is no genetic divergence and geographical differentiation between the North China Sea (CD, DL, and RZ) and the Southeast China Sea (HK and ST). Our results show no significant genetic differentiation between samples from the North China Sea and the Southeast China Sea. High dispersal potential of larvae by passive drift with ocean currents may explain the lack of genetic differentiation between samples. The results suggest a weak level of genetic structure in A. pectinate with a long planktonic larval stage.
{"title":"Genetic population structure of the pen shell Atrina pectinata along the coastlines of China revealed by microsatellites","authors":"Peican Zhu, Fukai Wang, Biao Wu, Feng Wang, Chunde Wang, Xiaotong Zhang, Kai Yu, Bo Liu","doi":"10.46989/001c.92111","DOIUrl":"https://doi.org/10.46989/001c.92111","url":null,"abstract":"An ecologically and economically important species in East Asia, the natural resources of pen shell Atrina pectinate have suffered severe population declines due to habitat destruction, pollution, and overfishing. Assessing genetic diversity and population structure is the basis for establishing conservation programs for A. pectinate. Our data indicated that high genetic diversity was found in all six populations, with mean allelic richness (Ar) ranging from 8.255 to 9.734, mean observed heterozygosity (Ho) from 0.574 to 0.680, and mean expected heterozygosity (He) from 0.620 to 0.691. The five A. pectinate populations were divided into two clusters. This clustering result was partly consistent with their geographical origin; the RZ population did not cluster with the northern populations (DL, CD), suggesting that there is no genetic divergence and geographical differentiation between the North China Sea (CD, DL, and RZ) and the Southeast China Sea (HK and ST). Our results show no significant genetic differentiation between samples from the North China Sea and the Southeast China Sea. High dispersal potential of larvae by passive drift with ocean currents may explain the lack of genetic differentiation between samples. The results suggest a weak level of genetic structure in A. pectinate with a long planktonic larval stage.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"60 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139527364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manh Van Ngo, Dung Van Tran, Thuy Thi Nguyen, Hung Quoc Pham
Salinity is crucial in fish larval rearing. In longfin batfish (Platax teira), little research has been conducted regarding the specific effects of salinity on growth, survival, deformity, and food utilization efficiency. This study aimed to determine the optimal salinity level for larval rearing of the longfin batfish by testing five different salinity levels (10, 15, 20, 25, and 30‰). Larvae of 1.5 cm in length and 0.2 g/fish in weight were stocked in cylindrical fiberglass tanks (300 L) at a density of 1 fish/L. The fish were fed to meet their dietary requirement and divided into four daily feedings. Each treatment was replicated three times over a 28-day period of rearing. The results revealed that salinity significantly influenced the growth (length, weight, biomass), and food utilization efficiency of the longfin batfish larvae. Overall, larvae reared at salinity levels of 15-20‰ exhibited superior performance compared to those exposed to salinity levels of 10, 25, and 30‰. However, salinity did not affect the coefficient of variation, survival, and deformity. From these findings, it is recommended to rear longfin batfish larvae at a salinity of 15-20‰ to achieve optimal growth and food utilization efficiency. This study provides valuable insights for longfin batfish larval rearing guidance, contributing to the aquaculture development of this economically valuable species.
{"title":"Growth, survival and food utilization efficiency of longfin batfish (Platax teira Forsskål, 1775) larvae reared under different salinity levels","authors":"Manh Van Ngo, Dung Van Tran, Thuy Thi Nguyen, Hung Quoc Pham","doi":"10.46989/001c.92092","DOIUrl":"https://doi.org/10.46989/001c.92092","url":null,"abstract":"Salinity is crucial in fish larval rearing. In longfin batfish (Platax teira), little research has been conducted regarding the specific effects of salinity on growth, survival, deformity, and food utilization efficiency. This study aimed to determine the optimal salinity level for larval rearing of the longfin batfish by testing five different salinity levels (10, 15, 20, 25, and 30‰). Larvae of 1.5 cm in length and 0.2 g/fish in weight were stocked in cylindrical fiberglass tanks (300 L) at a density of 1 fish/L. The fish were fed to meet their dietary requirement and divided into four daily feedings. Each treatment was replicated three times over a 28-day period of rearing. The results revealed that salinity significantly influenced the growth (length, weight, biomass), and food utilization efficiency of the longfin batfish larvae. Overall, larvae reared at salinity levels of 15-20‰ exhibited superior performance compared to those exposed to salinity levels of 10, 25, and 30‰. However, salinity did not affect the coefficient of variation, survival, and deformity. From these findings, it is recommended to rear longfin batfish larvae at a salinity of 15-20‰ to achieve optimal growth and food utilization efficiency. This study provides valuable insights for longfin batfish larval rearing guidance, contributing to the aquaculture development of this economically valuable species.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139618672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The hepatopancreas of Procambarus clarkii provides lipids and other nutrients for ovarian development and contains hormones such as estradiol related to ovarian development. During the development of the P. clarkii ovary, the hepatopancreas’ tissue structure and fatty acid content undergo corresponding changes. A controlled indoor experiment was conducted to investigate the effects of exogenous estradiol injection on the tissue structure and fatty acid content in the hepatopancreas of P. clarkii. The experiment consists of three experimental groups and one control group. The P. clarkii in the experimental group were injected with 5, 0.5, and 0.05 μg/(g body weight) of estradiol once every 5 days for 5 times and with normal saline injection as the control. After 25 days, the content of fatty acids and changes in tissue structure in the hepatopancreas were measured. The results showed that the main fatty acids in P. clarkii hepatopancreas were C18:4n, C20:3n, and EPA, and polyunsaturated fatty acid (PUFA) content was 57.17%±4.76%. Injecting estradiol mainly affected the content of C16:0, C16:1n, C18:0, C18:1n9, C18:2n, C18:3n6, C18:3n6, C18:4n, C20:1n, C20:2n, C20:3n, C20:4n3, C20:5n3, C22:n (n=1-4), C22:5n, and C22:6n in the hepatopancreas. Injecting estradiol significantly reduced the total content of PUFA in hepatopancreas with the reduction of C18:3n6, C18:4n, C20:3n, C20:4n3, C20:5n3 C22: n (n=1-4) (P<0.05); Estradiol injection significantly increased the total content of monounsaturated fatty acid (MUFA) in hepatopancreas with the increase of C18:1n9, and C20:1n (P<0.05); Injection of estradiol (0.05 μg/g) significantly increased the total content of saturated fatty acids (SFA) in the hepatopancreas (P<0.05) with the increase of C16:0 and C18:0 (P<0.05). Injecting estradiol can increase the volume and quantity of hepatopancreatic B cells, and the 5 μg/g group had the most obvious changes.
{"title":"Effects of estradiol on fatty acid composition and tissue structure of hepatopancreas in Procambarus clarkii","authors":"Weihong Zhao, Jintao Liu, Xirui Zheng, L. Lv, Jiyi Chen, Jiamei Wang, Yanming Sui, Liang Zheng","doi":"10.46989/001c.91079","DOIUrl":"https://doi.org/10.46989/001c.91079","url":null,"abstract":"The hepatopancreas of Procambarus clarkii provides lipids and other nutrients for ovarian development and contains hormones such as estradiol related to ovarian development. During the development of the P. clarkii ovary, the hepatopancreas’ tissue structure and fatty acid content undergo corresponding changes. A controlled indoor experiment was conducted to investigate the effects of exogenous estradiol injection on the tissue structure and fatty acid content in the hepatopancreas of P. clarkii. The experiment consists of three experimental groups and one control group. The P. clarkii in the experimental group were injected with 5, 0.5, and 0.05 μg/(g body weight) of estradiol once every 5 days for 5 times and with normal saline injection as the control. After 25 days, the content of fatty acids and changes in tissue structure in the hepatopancreas were measured. The results showed that the main fatty acids in P. clarkii hepatopancreas were C18:4n, C20:3n, and EPA, and polyunsaturated fatty acid (PUFA) content was 57.17%±4.76%. Injecting estradiol mainly affected the content of C16:0, C16:1n, C18:0, C18:1n9, C18:2n, C18:3n6, C18:3n6, C18:4n, C20:1n, C20:2n, C20:3n, C20:4n3, C20:5n3, C22:n (n=1-4), C22:5n, and C22:6n in the hepatopancreas. Injecting estradiol significantly reduced the total content of PUFA in hepatopancreas with the reduction of C18:3n6, C18:4n, C20:3n, C20:4n3, C20:5n3 C22: n (n=1-4) (P<0.05); Estradiol injection significantly increased the total content of monounsaturated fatty acid (MUFA) in hepatopancreas with the increase of C18:1n9, and C20:1n (P<0.05); Injection of estradiol (0.05 μg/g) significantly increased the total content of saturated fatty acids (SFA) in the hepatopancreas (P<0.05) with the increase of C16:0 and C18:0 (P<0.05). Injecting estradiol can increase the volume and quantity of hepatopancreatic B cells, and the 5 μg/g group had the most obvious changes.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":" 73","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139619385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mud crabs (Scylla genus) are luxury foods in high demand internationally. The efficient techniques for mud crab hatcheries are vital for providing breeds for their aquaculture, which is rapidly growing in many countries. This study aims to investigate the effects of different feeds and stocking densities on mud crabs’ growth and survival rates (Scylla paramamosain) in the stage from megalopa to crablet-1 stage. Two separate experiments were conducted indoors in the 60-liter round plastic tanks (containing 50 liters of water at a 28‰ salinity). Experiment 1 investigated four feeds: frozen Artemia biomass, pureed shrimp meat, Lansy pellet feed (48% protein), and NRD pellet feed (55% protein). Megalopae (mean weight of 5.8 mg) were stocked at a density of 10/L. In experiment 2, the megalopae (mean weight of 5.4 mg) were stocked at densities of 20, 30, and 40/L and were fed the Lansy pellet feed, which was the best one selected from experiment 1. High survival rates were obtained at all four feeds (82.2–87.5%) and three stocking densities (88.4–90.1%). The growth performances in Lansy feed and frozen Artemia biomass were better than those in pureed shrimp meat and NRD pellet feed, which was seen through higher indicators of daily weight gain (DWG) and specific growth rate in weight (SGRw) (p < 0.05). Despite the survival rate showing no significant difference among the feeds, their highest value (87.5%) was observed in the Lansy pellet feed. On the other hand, the growth performances showed a significant decrease at stocking densities ≥ 30/L, as complemented by a significant decrease in DWG and SGRw at these stocking densities (p < 0.05). Both survival rate and metamorphosis durations did not show significant differences among the feeds or stocking densities (p > 0.05). The investigated feeds and stocking densities suit the nursing mud crab (S. paramamosain) megalopa. In contrast, the Lansy pellet feeds had a stocking density of 20/L, resulting in the highest nursing efficiency.
{"title":"Effects of different feeds and stocking densities on growth and survival rates of mud crab (Scylla paramamosain) at the stage from megalopa to crablet-1","authors":"Tien Hai Ly, Le Hoang Vu, D. X. Diep","doi":"10.46989/001c.91128","DOIUrl":"https://doi.org/10.46989/001c.91128","url":null,"abstract":"Mud crabs (Scylla genus) are luxury foods in high demand internationally. The efficient techniques for mud crab hatcheries are vital for providing breeds for their aquaculture, which is rapidly growing in many countries. This study aims to investigate the effects of different feeds and stocking densities on mud crabs’ growth and survival rates (Scylla paramamosain) in the stage from megalopa to crablet-1 stage. Two separate experiments were conducted indoors in the 60-liter round plastic tanks (containing 50 liters of water at a 28‰ salinity). Experiment 1 investigated four feeds: frozen Artemia biomass, pureed shrimp meat, Lansy pellet feed (48% protein), and NRD pellet feed (55% protein). Megalopae (mean weight of 5.8 mg) were stocked at a density of 10/L. In experiment 2, the megalopae (mean weight of 5.4 mg) were stocked at densities of 20, 30, and 40/L and were fed the Lansy pellet feed, which was the best one selected from experiment 1. High survival rates were obtained at all four feeds (82.2–87.5%) and three stocking densities (88.4–90.1%). The growth performances in Lansy feed and frozen Artemia biomass were better than those in pureed shrimp meat and NRD pellet feed, which was seen through higher indicators of daily weight gain (DWG) and specific growth rate in weight (SGRw) (p < 0.05). Despite the survival rate showing no significant difference among the feeds, their highest value (87.5%) was observed in the Lansy pellet feed. On the other hand, the growth performances showed a significant decrease at stocking densities ≥ 30/L, as complemented by a significant decrease in DWG and SGRw at these stocking densities (p < 0.05). Both survival rate and metamorphosis durations did not show significant differences among the feeds or stocking densities (p > 0.05). The investigated feeds and stocking densities suit the nursing mud crab (S. paramamosain) megalopa. In contrast, the Lansy pellet feeds had a stocking density of 20/L, resulting in the highest nursing efficiency.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139439122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu-Qing Zhang, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, K. Zhu, Dian-chang Zhang
To explore the morphological and phenotypic characteristics and differences among different populations of Larimichthys crocea, traditional morphological measurements were carried out on three wild populations from Zhoushan, Xiamen and Zhanjiang and two farmed populations from Ningde and Wenzhou. Seven morphological parameters of five L. crocea populations were compared and analyzed. The results of one-way ANOVA showed significant differences in trunk and caudal stalk among the five populations. The contribution rates of the first five principal components to the total difference among different populations were 29.984%, 18.462%, 17.234%, 12.167%, and 9.904%, respectively, and the cumulative contribution rates were 87.751%. Trunk can be used to distinguish different geographic populations best. The cluster analysis results showed that the distance between wild populations was the closest, while the distance between farmed populations was far. The step discriminant method established the classification discriminant function of 5 populations. The discriminant accuracy P1 was 78.3%-92.7%, the discriminant accuracy P2 was 76.4%-96.5%, and the comprehensive recognition rate was 99.3%. The discriminant accuracy of this method was high, and it could provide a reference for the differentiation of different populations of L. crocea. This study provided basic morphological data for identifying a large yellow croaker population, protecting germplasm resources, and breeding improved varieties.
{"title":"Analysis of morphological differences in five large yellow croaker (Larimichthys crocea) populations","authors":"Yu-Qing Zhang, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, K. Zhu, Dian-chang Zhang","doi":"10.46989/001c.91049","DOIUrl":"https://doi.org/10.46989/001c.91049","url":null,"abstract":"To explore the morphological and phenotypic characteristics and differences among different populations of Larimichthys crocea, traditional morphological measurements were carried out on three wild populations from Zhoushan, Xiamen and Zhanjiang and two farmed populations from Ningde and Wenzhou. Seven morphological parameters of five L. crocea populations were compared and analyzed. The results of one-way ANOVA showed significant differences in trunk and caudal stalk among the five populations. The contribution rates of the first five principal components to the total difference among different populations were 29.984%, 18.462%, 17.234%, 12.167%, and 9.904%, respectively, and the cumulative contribution rates were 87.751%. Trunk can be used to distinguish different geographic populations best. The cluster analysis results showed that the distance between wild populations was the closest, while the distance between farmed populations was far. The step discriminant method established the classification discriminant function of 5 populations. The discriminant accuracy P1 was 78.3%-92.7%, the discriminant accuracy P2 was 76.4%-96.5%, and the comprehensive recognition rate was 99.3%. The discriminant accuracy of this method was high, and it could provide a reference for the differentiation of different populations of L. crocea. This study provided basic morphological data for identifying a large yellow croaker population, protecting germplasm resources, and breeding improved varieties.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"27 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139384784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Tu, Nguyen Thi Kim Lien, D. X. Diep, Tien Hai Ly
This study evaluated the effects of ginger extract as a feed additive on the growth performance, digestive enzymes, and resistance to environmental stress of the whiteleg shrimp, Litopenaeus vannamei. 1200 juveniles were randomly allocated into four groups with three replicates. Shrimps were fed diets supplemented with 0 (T0-control), 0.5 (T1), 1 (T2), and 1.5 g kg-1 of ginger extract (T3) for 56 days. The results indicated that shrimp fed with ginger extract had a lower feed conversion ratio (FCR) than the control. In addition, shrimp fed with 0.5 g kg-1 of ginger extract had significantly greater weight gain and protease activity in the intestine than those in the control group. However, dietary supplementation with ginger extract did not significantly affect survival rates. After 56 days of culture, shrimp fed with 0.5 and 1 g kg-1 of ginger extract had higher survival rates than the controls after 72 – 96 h exposure to high ammonia stress (40 mg L-1). Based on the study’s findings, ginger extract could be recommended for shrimp feed to enhance growth and resistance against stress factors, and the optimal level is 0.5 g kg-1.
本研究评估了生姜提取物作为饲料添加剂对南美白对虾（Litopenaeus vannamei）生长性能、消化酶和抗环境胁迫能力的影响。将 1200 尾幼体随机分为四组，每组三个重复。分别投喂添加 0（T0-对照组）、0.5（T1）、1（T2）和 1.5 g kg-1 生姜提取物（T3）的日粮 56 天。结果表明，用生姜提取物喂养的对虾的饲料转化率（FCR）低于对照组。此外，喂食 0.5 g kg-1 生姜提取物的对虾的增重和肠道蛋白酶活性明显高于对照组。然而，膳食中添加生姜提取物对成活率没有明显影响。养殖 56 天后，喂食 0.5 和 1 g kg-1 生姜提取物的对虾在暴露于高氨胁迫（40 mg L-1）72-96 小时后的存活率高于对照组。根据研究结果，生姜提取物可推荐用于对虾饲料，以提高对虾的生长和抗应激能力，最佳添加量为 0.5 g kg-1。
{"title":"Effect of ginger, Zingiber officinale extract on growth performance, digestive enzyme and stress tolerance of whiteleg shrimp, Litopenaeus vannamei juveniles","authors":"P. Tu, Nguyen Thi Kim Lien, D. X. Diep, Tien Hai Ly","doi":"10.46989/001c.90973","DOIUrl":"https://doi.org/10.46989/001c.90973","url":null,"abstract":"This study evaluated the effects of ginger extract as a feed additive on the growth performance, digestive enzymes, and resistance to environmental stress of the whiteleg shrimp, Litopenaeus vannamei. 1200 juveniles were randomly allocated into four groups with three replicates. Shrimps were fed diets supplemented with 0 (T0-control), 0.5 (T1), 1 (T2), and 1.5 g kg-1 of ginger extract (T3) for 56 days. The results indicated that shrimp fed with ginger extract had a lower feed conversion ratio (FCR) than the control. In addition, shrimp fed with 0.5 g kg-1 of ginger extract had significantly greater weight gain and protease activity in the intestine than those in the control group. However, dietary supplementation with ginger extract did not significantly affect survival rates. After 56 days of culture, shrimp fed with 0.5 and 1 g kg-1 of ginger extract had higher survival rates than the controls after 72 – 96 h exposure to high ammonia stress (40 mg L-1). Based on the study’s findings, ginger extract could be recommended for shrimp feed to enhance growth and resistance against stress factors, and the optimal level is 0.5 g kg-1.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"20 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wei Cao, Mingyi Zhang, Nan Wu, Haiying Han, Rujie Zhong, Tao Yu, Xiaomei Wang, Liqun Ren, Chunnuan Zhao, Bo Li, Yanxin Zheng
The black rockfish Sebastes schlegelii is an important fishery species in Japan, South Korea, and China. Overfishing has severely depleted the natural resources of S. schlegelii in recent years, leading to the initiation of programs aimed at enhancing fish stock. However, the genetic structure of northern populations remains elusive, posing challenges in collecting and preserving germplasm resources. In this study, a total of 191 S. schlegelii individuals from seven populations, including one cultured population (Changdao: CDYZ) and six wild populations (Lianyungang: LYG; Qingdao: QD; Weihai: WH; Changdao: CDYS; Beidaihe: BDH) sequenced by 2b-RAD method and their population genetics was analyzed using 27,064 SNPs obtained. The results indicated low genetic diversity in both wild and cultured populations (PIC < 0.25, Ho: 0.174-0.273, He: 0.173-0.234), with the cultured population exhibiting higher diversity than the wild ones. Moderate genetic differentiation existed between the cultured population and six wild populations (0.05 < Fst < 0.25). However, the genetic differentiation was lower among the other wild populations (Fst < 0.05). The analyses of population genetic structure indicated that the wild populations clustered together first and finally with the cultured population CDYZ, which clustered separately except for one individual. Therefore, scientific breeding programs and germplasm conservation can be important in stabilizing genetic structure. In addition, gene flow among the seven populations was high (Nm > 1). This study provides a theoretical basis for conserving and rationalizing germplasm resources for S. schlegelii.
{"title":"Population genetics analysis of the black rockfish Sebastes schlegelii in Northern China based on 2b-RAD simplified genome sequencing","authors":"Wei Cao, Mingyi Zhang, Nan Wu, Haiying Han, Rujie Zhong, Tao Yu, Xiaomei Wang, Liqun Ren, Chunnuan Zhao, Bo Li, Yanxin Zheng","doi":"10.46989/001c.90864","DOIUrl":"https://doi.org/10.46989/001c.90864","url":null,"abstract":"The black rockfish Sebastes schlegelii is an important fishery species in Japan, South Korea, and China. Overfishing has severely depleted the natural resources of S. schlegelii in recent years, leading to the initiation of programs aimed at enhancing fish stock. However, the genetic structure of northern populations remains elusive, posing challenges in collecting and preserving germplasm resources. In this study, a total of 191 S. schlegelii individuals from seven populations, including one cultured population (Changdao: CDYZ) and six wild populations (Lianyungang: LYG; Qingdao: QD; Weihai: WH; Changdao: CDYS; Beidaihe: BDH) sequenced by 2b-RAD method and their population genetics was analyzed using 27,064 SNPs obtained. The results indicated low genetic diversity in both wild and cultured populations (PIC < 0.25, Ho: 0.174-0.273, He: 0.173-0.234), with the cultured population exhibiting higher diversity than the wild ones. Moderate genetic differentiation existed between the cultured population and six wild populations (0.05 < Fst < 0.25). However, the genetic differentiation was lower among the other wild populations (Fst < 0.05). The analyses of population genetic structure indicated that the wild populations clustered together first and finally with the cultured population CDYZ, which clustered separately except for one individual. Therefore, scientific breeding programs and germplasm conservation can be important in stabilizing genetic structure. In addition, gene flow among the seven populations was high (Nm > 1). This study provides a theoretical basis for conserving and rationalizing germplasm resources for S. schlegelii.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"82 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139178476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yihao Wang, Yu Yuan, Kun Peng, Yilin Wang, Longjun Deng, Tiancai Li, Defang Chen, Ouyang Ping, Xiaoli Huang, Hongrui Guo, H. Deng, W. Lai, Yi Geng
Streptococcus agalactiae (GBS) is an important Gram-positive pathogen of fish aquaculture worldwide. In this study, we performed a comparative analysis of GBS S03 (serotype III) and S07 (serotype Ia) isolated from Schizothorax spp.. We explored the association between phenotypic antibiotic resistance, virulence, and genomic characteristics. Antimicrobial sensitivity tests on 12 common antibiotics using the disc diffusion method revealed that the GBS S03 resisted seven antibiotics, while GBS S07 showed sensitivity to eleven antibiotics. Pathogenicity analysis demonstrated greater virulence of GBS S07 than S03. Then, the occurrence of antibiotic resistance and virulence genes were identified using the whole-genome sequence (WGS) of GBS S03 and GBS S07. Besides just GBS S03 had gyrA and parC gene mutations, there were mefE, tetO, lnuB, lsaE, APH3’, and sat-4 resistance genes present only in GBS S03 genome. In addition to 51 virulence genes in both GBS S03 and S07 genomes, GBS S07 carried virulence genes associated with invasion, such as SAN_1519, rfbA and cylE genes. There was complete concordance between genotypic evidence and phenotypic characteristics. Virulence factors and phylogenetic analysis showed that GBS S03 and human sources shared an extremely close evolutionary relationship. Our findings also provide important proof for using WGS as an effective tool for phenotypic predictions of GBS.
{"title":"Comparative analysis of Streptococcus agalactiae S03 and S07 isolated from Schizothorax spp. with different antibiotic resistance and virulence","authors":"Yihao Wang, Yu Yuan, Kun Peng, Yilin Wang, Longjun Deng, Tiancai Li, Defang Chen, Ouyang Ping, Xiaoli Huang, Hongrui Guo, H. Deng, W. Lai, Yi Geng","doi":"10.46989/001c.90498","DOIUrl":"https://doi.org/10.46989/001c.90498","url":null,"abstract":"Streptococcus agalactiae (GBS) is an important Gram-positive pathogen of fish aquaculture worldwide. In this study, we performed a comparative analysis of GBS S03 (serotype III) and S07 (serotype Ia) isolated from Schizothorax spp.. We explored the association between phenotypic antibiotic resistance, virulence, and genomic characteristics. Antimicrobial sensitivity tests on 12 common antibiotics using the disc diffusion method revealed that the GBS S03 resisted seven antibiotics, while GBS S07 showed sensitivity to eleven antibiotics. Pathogenicity analysis demonstrated greater virulence of GBS S07 than S03. Then, the occurrence of antibiotic resistance and virulence genes were identified using the whole-genome sequence (WGS) of GBS S03 and GBS S07. Besides just GBS S03 had gyrA and parC gene mutations, there were mefE, tetO, lnuB, lsaE, APH3’, and sat-4 resistance genes present only in GBS S03 genome. In addition to 51 virulence genes in both GBS S03 and S07 genomes, GBS S07 carried virulence genes associated with invasion, such as SAN_1519, rfbA and cylE genes. There was complete concordance between genotypic evidence and phenotypic characteristics. Virulence factors and phylogenetic analysis showed that GBS S03 and human sources shared an extremely close evolutionary relationship. Our findings also provide important proof for using WGS as an effective tool for phenotypic predictions of GBS.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139178128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study aimed to assess Nile tilapia’s (Oreochromis niloticus) growth performance and body chemical composition through varying levels of L-Glutamic acid supplementation. In the experiment, four different groups of feed were prepared; the control C (0% L-Glutamic acid), G1 (1% L-Glutamic acid), G2 (2% L-Glutamic acid), and G3 (3% L-Glutamic acid). Tilapia juveniles with an average initial weight of 4.86 g were stocked into 12 fiberglass tanks (450L) with 40 individuals and three replications under controlled conditions, and the experiment was continued for a period of 60 days. At the end of the experiment, for the G2 group final weight (FW, 19.31±0.59 g), specific growth rate (SGR; 2.30±0.05), feed conversion ratio (FCR; 1.02±0.03), daily growth rate (DGR; 4.95±0.20) protein efficiency ratio (PER 2.83±0.10) and net protein utilization (NPU; 61.62±3.39) were better than the other groups (P<0.05). Nutritional composition data showed that the groups differed between protein, dry matter, and lipid compositions. The G2 group exhibited the highest whole-body protein level, recording a value of 21.24±0.52, whereas the control group demonstrated the lowest protein level at 20.17±0.15. In conclusion, incorporating 2% L-Glutamic acid into the diet of juvenile Nile tilapia is advisable for both the growth and development of the fish and for enhancing their nutritional composition.
{"title":"Determination of Growth and Nutritional Composition of Nile Tilapia (Oreochromis niloticus) Fed With L-Glutamic Acid Supplemented Feeds","authors":"Yılmaz Dağdelen, O. Taşbozan","doi":"10.46989/001c.90850","DOIUrl":"https://doi.org/10.46989/001c.90850","url":null,"abstract":"The study aimed to assess Nile tilapia’s (Oreochromis niloticus) growth performance and body chemical composition through varying levels of L-Glutamic acid supplementation. In the experiment, four different groups of feed were prepared; the control C (0% L-Glutamic acid), G1 (1% L-Glutamic acid), G2 (2% L-Glutamic acid), and G3 (3% L-Glutamic acid). Tilapia juveniles with an average initial weight of 4.86 g were stocked into 12 fiberglass tanks (450L) with 40 individuals and three replications under controlled conditions, and the experiment was continued for a period of 60 days. At the end of the experiment, for the G2 group final weight (FW, 19.31±0.59 g), specific growth rate (SGR; 2.30±0.05), feed conversion ratio (FCR; 1.02±0.03), daily growth rate (DGR; 4.95±0.20) protein efficiency ratio (PER 2.83±0.10) and net protein utilization (NPU; 61.62±3.39) were better than the other groups (P<0.05). Nutritional composition data showed that the groups differed between protein, dry matter, and lipid compositions. The G2 group exhibited the highest whole-body protein level, recording a value of 21.24±0.52, whereas the control group demonstrated the lowest protein level at 20.17±0.15. In conclusion, incorporating 2% L-Glutamic acid into the diet of juvenile Nile tilapia is advisable for both the growth and development of the fish and for enhancing their nutritional composition.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"4 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139178466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hui Liu, Zhuo Pei, Ke Fan, Yuanyi Liu, Yang Wang, Yumei Liu, Yong Huang
This study aimed to understand the effect of various levels of waterborne cadmium exposure and associated integrated response in largemouth bass (Micropterus salmoides). The experimental fish were randomly divided into 4 groups: Group H (high concentration group): 0.25 mg/L of water (100% of the LC50); Group M (medium concentration group): 0.01 mg/L of water (50% of the LC50); group L (low concentration group): 0.05 mg/L of water (12.5% of the LC50) and group C (control). Experimental fish in group C (control group) were cultured in cadmium (Cd)-free tap water. After 28 days of Cd exposure, the gill and liver tissues were collected for histological analysis and antioxidant enzyme activities assay. Serum biochemical indices and the expression levels of HSP70, HSP90, Cyp1a, and MT in the liver were assayed. Results indicated Cd exposure in group H could significantly damage the gill and liver. The SOD activity of gill and liver exhibited an increasing trend with Cd concentrations increasing, and SOD activities increased significantly in group M compared to that of group C. Still, SOD activity significantly decreased in group H. MDA content increased significantly in both the gill and liver, while T-AOC activities were not significantly different in both the gill and liver. By comparison with group C, the activities of ALT and AST in serum were significantly increased in group M and group H; the contents of ALB and TP significantly decreased in group M and group H except for the content slightly increased in the group L; the contents of GLU, TG, and TC did not show significant changes in the group L and the group M, but increased significantly in the group H. The expression levels of HSP70 and HSP90 in the liver exhibited the highest level in group M, whereas the expression levels of MT and Cyp1a were highest in groups H and L, respectively. Our results are intended to provide a reference basis for the safety risk evaluation of Cd and healthy fish farming.
本研究旨在了解大口鲈鱼（Micropterus salmoides）暴露于不同水平水体镉的影响及相关综合反应。实验鱼被随机分为 4 组：H组（高浓度组）：0.01毫克/升水（LC50的50%）；L组（低浓度组）：L 组（低浓度组）：0.05 毫克/升水（LC50 的 12.5%）和 C 组（对照组）。C 组（对照组）的实验鱼在无镉（Cd）的自来水中养殖。镉暴露 28 天后，收集鳃和肝组织进行组织学分析和抗氧化酶活性检测。检测血清生化指标以及肝脏中 HSP70、HSP90、Cyp1a 和 MT 的表达水平。结果表明，H 组的镉暴露会对鳃和肝脏造成严重损害。随着镉浓度的增加，鳃和肝脏的 SOD 活性呈上升趋势，与 C 组相比，M 组的 SOD 活性显著增加。与 C 组相比，血清中 ALT 和 AST 的活性在 M 组和 H 组明显升高；ALB 和 TP 的含量在 M 组和 H 组明显降低，只有 L 组略有升高；GLU、TG 和 TC 的含量在 L 组和 M 组没有明显变化，但在 H 组明显升高。我们的研究结果旨在为镉的安全风险评估和健康养鱼提供参考依据。
{"title":"Effects of cadmium exposure on tissue structure, antioxidant enzyme activity, serum biochemical indices and gene expression in the liver of juvenile largemouth bass (Micropterus salmoides)","authors":"Hui Liu, Zhuo Pei, Ke Fan, Yuanyi Liu, Yang Wang, Yumei Liu, Yong Huang","doi":"10.46989/001c.90334","DOIUrl":"https://doi.org/10.46989/001c.90334","url":null,"abstract":"This study aimed to understand the effect of various levels of waterborne cadmium exposure and associated integrated response in largemouth bass (Micropterus salmoides). The experimental fish were randomly divided into 4 groups: Group H (high concentration group): 0.25 mg/L of water (100% of the LC50); Group M (medium concentration group): 0.01 mg/L of water (50% of the LC50); group L (low concentration group): 0.05 mg/L of water (12.5% of the LC50) and group C (control). Experimental fish in group C (control group) were cultured in cadmium (Cd)-free tap water. After 28 days of Cd exposure, the gill and liver tissues were collected for histological analysis and antioxidant enzyme activities assay. Serum biochemical indices and the expression levels of HSP70, HSP90, Cyp1a, and MT in the liver were assayed. Results indicated Cd exposure in group H could significantly damage the gill and liver. The SOD activity of gill and liver exhibited an increasing trend with Cd concentrations increasing, and SOD activities increased significantly in group M compared to that of group C. Still, SOD activity significantly decreased in group H. MDA content increased significantly in both the gill and liver, while T-AOC activities were not significantly different in both the gill and liver. By comparison with group C, the activities of ALT and AST in serum were significantly increased in group M and group H; the contents of ALB and TP significantly decreased in group M and group H except for the content slightly increased in the group L; the contents of GLU, TG, and TC did not show significant changes in the group L and the group M, but increased significantly in the group H. The expression levels of HSP70 and HSP90 in the liver exhibited the highest level in group M, whereas the expression levels of MT and Cyp1a were highest in groups H and L, respectively. Our results are intended to provide a reference basis for the safety risk evaluation of Cd and healthy fish farming.","PeriodicalId":506439,"journal":{"name":"Israeli Journal of Aquaculture - Bamidgeh","volume":"92 7-8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139181363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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