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Analysis of environmental factors influencing lumpy skin disease outbreak seasonality and assessment of its spread risk in the Saratovskaya oblast of Russia 影响俄罗斯萨拉托夫州块状皮肤病爆发季节性的环境因素分析及其传播风险评估
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.630-644
Dmitry Podshibyakin, L. Padilo, V. Agoltsov, O. Chernykh, Olga Popova, Kalabekov Mutalif, N. Solotova
Background and Aim: Lumpy skin disease (LSD) is a transboundary viral disease of cattle that causes serious economic losses due to a significant decrease in meat and milk productivity. This study analyzed the influence of natural and anthropogenic environmental factors on LSD spread seasonality and assessed the risk of LSD outbreaks in the Saratovskaya oblast of the Russian Federation.Materials and Methods: Data on LSD outbreaks and environmental factors during different seasons were collected for the period 2011-2020 in the Balkan Peninsula, Middle East, and Russia. Risk assessment was performed using mathematical modeling with generalized linear regression and maximum entropy.Results: Fourteen statistically significant environmental factors influencing LSD spread were identified. The analysis of MaxEnt models built using the selected factors showed that the presence of the pathogen is mostly exerted by: the density of susceptible cattle (an increased risk is observed at a density above 10 and 20 heads/10 km2 in winter and autumn, with a permanent risk in spring and summer), the density of water bodies (the risk is increased at any density in winter and autumn, in the range of 13-23.5 m2/km2 in spring, in the ranges of 0-8 and over 14.5 m2/km2 in summer), and average monthly precipitation rate (the most risky are 105-185 mm/month in winter, 35 mm in spring, 15-105 mm in summer, and above 50 mm in autumn).Conclusion: LSD tends to spread during the warm season. Compared with other test zones, the Saratovskaya oblast has a negligible risk of disease spread (in winter), low risk (in spring), or medium risk (in summer and autumn). The annual risk is low to medium.Keywords: cattle, environmental factors, generalized linear regression, lumpy skin disease, maximum entropy, species distribution.
背景与目的:结节性皮肤病(LSD)是一种牛的跨境病毒性疾病,会导致肉类和牛奶产量显著下降,造成严重的经济损失。本研究分析了自然和人为环境因素对 LSD 传播季节性的影响,并评估了 LSD 在俄罗斯联邦萨拉托夫州爆发的风险:收集了 2011-2020 年期间巴尔干半岛、中东和俄罗斯不同季节的迷幻剂爆发和环境因素数据。采用广义线性回归和最大熵数学模型进行风险评估:结果:确定了影响 LSD 传播的 14 个具有统计学意义的环境因素。利用所选因素建立的 MaxEnt 模型分析表明,病原体的存在主要受到以下因素的影响:易感牛的密度(在冬季和秋季,当密度超过 10 头和 20 头/10 平方公里时,风险会增加,而在春季和夏季,风险永久不变)、水体密度(在冬季和秋季,任何密度的风险都会增加,在 13-23.5 m2/km2,夏季在 0-8 m2/km2 和 14.5 m2/km2 以上),以及月平均降水量(风险最大的是冬季 105-185 mm/月,春季 35 mm/月,夏季 15-105 mm/月,秋季 50 mm/月以上):结论:LSD 往往在温暖季节蔓延。与其他试验区相比,萨拉托夫州的病害传播风险可忽略不计(冬季)、低风险(春季)或中等风险(夏季和秋季)。关键词:牛、环境因素、广义线性回归、块状皮肤病、最大熵、物种分布。
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引用次数: 0
Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection 因嗜水气单胞菌感染而补充枯草芽孢杆菌和干酪乳杆菌以提高鲶鱼的生长性能和免疫力
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.602-611
Nurul Aini, Dini Sarafina Yulia Rosa Putri, Divany Hunaimatul Achhlam, Fatimah Fatimah, S. Andriyono, Dyah Hariani, Hoang Dang Khoa Do, S. Wahyuningsih
Background and Aim: Catfish has a high economic value and is popular among consumers. To ensure well-stocked catfish stocks, good fisheries management must also be ensured. The high demand for catfish must be supplemented by preventive measures against pathogenic bacterial infections using probiotics with high potential for Lactobacillus casei and Bacillus subtilis. The aim of this study was to determine the effect of probiotic supplementation consisting of a combination of L. casei and B. subtilis probiotics on the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with Aeromonas hydrophila.Materials and Methods: This study used a completely randomized study with eight treatments and three replications. The manipulated factor was the probiotic concentration [0% (A), 0.5% (B), 10% (C), and 15% (D)] in groups of catfish infected and uninfected with A. hydrophila. Combination of B. subtilis, and L. casei that were used in a 1:1 ratio of 108 colony forming unit/mL. The study lasted for 42 days. On the 35th day, A. hydrophila was infected by intramuscular injection into fish. The Statistical Package for the Social Sciences (SPSS) software version 23.0 (IBM SPSS Statistics) was used to analyze data on growth, immune system, and water quality.Results: Providing probiotics in feed can increase the nutritional value of feed based on proximate test results. There were significant differences in average daily gain (ADG), feed conversion ratio (FCR), and survival rate (SR) parameters in the group of catfish infected with A. hydrophila (p > 0.05); however, there were no significant differences in final body weight, specific growth rate (SGR), and percentage weight gain. Interleukin-1β (IL-1β) levels were significantly different between treatments C and D. The tumor necrosis factor (TNF) α parameters were significantly different between treatments A and C, whereas the phagocytic activity of treatment A was significantly different from that of treatment D. There was a significant difference (p > 0.05) in the growth parameters of SGR, ADG, and FCR in the group of fish that were not infected with A. hydrophila, with the best treatment being a probiotic concentration of 15%, but there was no significant difference in the SR parameters. IL-1β and TNF-α levels significantly differed between E and E0 (15% probiotics) but were not significantly different in terms of phagocytosis parameters.Conclusion: Based on the results of this study, it can be concluded that using a combination of probiotics L. casei and B. subtilis can improve the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with A. hydrophila.Keywords: catfish, fish stocks and fisheries management, growth parameters, immune response, probiotic.
背景和目的:鲶鱼具有很高的经济价值,深受消费者喜爱。为确保鲶鱼种群数量充足,还必须确保良好的渔业管理。为了满足对鲶鱼的高需求,必须使用具有高潜力的乳酸杆菌和枯草芽孢杆菌等益生菌来预防病原菌感染。本研究旨在确定由干酪乳杆菌和枯草芽孢杆菌益生菌组合组成的益生菌补充剂对感染嗜水气单胞菌的鲶鱼的生长、免疫系统、水质、饲料近似值和身体成分的影响:本研究采用完全随机研究法,共有八个处理和三次重复。处理因子为益生菌浓度[0%(A)、0.5%(B)、10%(C)和 15%(D)]。枯草芽孢杆菌和干酪乳杆菌以 1:1 的比例混合使用,菌落形成单位为 108 个/毫升。研究持续了 42 天。第 35 天,通过肌肉注射使鱼感染蚜虫。使用社会科学统计软件包(SPSS)软件 23.0 版(IBM SPSS Statistics)分析生长、免疫系统和水质数据:根据近似测试结果,在饲料中添加益生菌可提高饲料的营养价值。感染虹彩蓑衣藻的鲶鱼组在平均日增重(ADG)、饲料转化率(FCR)和存活率(SR)参数上存在显著差异(P > 0.05);但在最终体重、特定生长率(SGR)和增重百分比上没有显著差异。白细胞介素-1δ(IL-1δ)水平在处理 C 和处理 D 之间存在显著差异;肿瘤坏死因子(TNF)δ参数在处理 A 和处理 C 之间存在显著差异,而处理 A 的吞噬活性与处理 D 存在显著差异。未感染嗜水青虫的鱼类组在 SGR、ADG 和 FCR 等生长参数上有显著差异(p > 0.05),其中益生菌浓度为 15%的处理效果最好,但在 SR 参数上没有显著差异。E和E0(15%益生菌)的IL-1δ²和TNF-δ±水平有显著差异,但在吞噬作用参数方面没有显著差异:根据本研究的结果,可以得出结论:联合使用益生菌 L. casei 和 B. subtilis 可以改善感染鳗鲡的鲶鱼的生长、免疫系统、水质、饲料的近似值和体质。
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引用次数: 0
Antimicrobial resistance profiles of Escherichia coli derived from an integrated agroforestry-livestock system in Deli Serdang Regency, Indonesia 印度尼西亚 Deli Serdang 地区农林牧综合系统中大肠杆菌的抗菌药耐药性概况
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.690-699
R. R. Dewi, A. Nuryawan, S. M. Jajere, J. M. Sihombing, I. J. Tambunan
Background and Aim: Antimicrobial resistance (AMR) has become a significant global concern. Epidemiological data do not provide a robust description of the potential risks associated with AMR in the integrated agroforestry-livestock systems in Indonesia. Thus, the present study investigated the phenotypic and multidrug resistance (MDR) profiles of Escherichia coli strains isolated from the feces of livestock raised in the agro-silvopastoral system in Deli Serdang Regency, North Sumatra Province.Materials and Methods: A standard microbiological culture procedure was followed to isolate the organism and test antibiotic susceptibility using the Kirby-Bauer disk diffusion protocol. Furthermore, the multiple antibiotic resistance index was determined. Univariate analysis was conducted to identify the risk factors associated with AMR.Results: The vast majority (77.5%) of livestock farmers were aged >30 years. All farmers were men and had no higher education (100% of them). The majority of the animal species managed were cattle and goats (37.5% each) and the livestock grazing pasture system (67.5%). In addition, the majority of farmers reported high antimicrobial use on their farms (87.5%). Of the samples (n = 142) analyzed, n = 70 were positive, with an overall prevalence of 44.4%. The species-specific prevalences of E. coli were 32.5%, 47.8%, and 50% in buffalo, goat, and cattle, respectively. Ampicillin and tetracyclines exhibited high resistance levels among the studied animal species. A relatively lower MDR for E. coli was associated with grazing on the pasture.Conclusion: The findings from the current study provide baseline epidemiological information for future robust studies aimed at elucidating the drivers and patterns of AMR in agro-silvopastoral systems in the study area or elsewhere.Keywords: agro-silvopastoral, antimicrobial Resistance, Escherichia coli,Indonesia, livestock, multidrug resistance.
背景与目的:抗菌素耐药性(AMR)已成为全球关注的一个重要问题。流行病学数据并未对印度尼西亚农林畜牧综合系统中与 AMR 相关的潜在风险提供有力的描述。因此,本研究调查了从北苏门答腊省德利瑟当地区农林牧系统饲养的牲畜粪便中分离出的大肠埃希氏菌株的表型和耐多药(MDR)特征:按照标准微生物培养程序分离菌体,并使用柯比鲍尔盘扩散法检测抗生素敏感性。此外,还测定了多重抗生素耐药性指数。进行单变量分析以确定与 AMR 相关的风险因素:绝大多数(77.5%)畜牧业者的年龄大于 30 岁。所有养殖户均为男性,且未受过高等教育(100%)。管理的动物种类大多是牛和山羊(各占 37.5%),以及牲畜放牧草场系统(占 67.5%)。此外,大多数农户称其农场使用大量抗菌剂(87.5%)。在分析的样本(n = 142)中,n = 70 呈阳性,总流行率为 44.4%。水牛、山羊和牛的大肠埃希氏菌特定物种流行率分别为 32.5%、47.8% 和 50%。在所研究的动物物种中,氨苄西林和四环素的耐药性水平较高。大肠杆菌的耐药性相对较低与在牧场放牧有关:本次研究的结果为今后旨在阐明该研究地区或其他地区农-硅-牧系统中 AMR 的驱动因素和模式的有力研究提供了流行病学基线信息。
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引用次数: 0
Apgar scores in puppies following the induction of etomidate compared with alfaxalone or propofol for cesarean section 剖腹产手术中使用依托咪酯与阿法沙龙或异丙酚诱导后幼犬的 Apgar 评分比较
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.527-534
T. Srithunyarat, S. Jitpean, Piyasak Wipoosak, Chalermkwan Nonthakotr, N. Boonbal, P. Kunkitti, S. Seesupa
Background and Aim: The Apgar score is a useful assessment of neonatal viability in dogs. The Apgar score in puppies born by cesarean section can be lower than vaginal delivery because all anesthetic drugs can cross the placenta. Therefore, anesthetic drugs with minimal cardiorespiratory effect and rapid elimination are recommended for cesarean section. The present study aimed to compare Apgar scores in puppies born after the induction of etomidate, alfaxalone or propofol, and those maintained with isoflurane inhalation during cesarean section.Materials and Methods: Thirty-six bitches were equally divided in the three anesthetic drug groups. Modified Apgar scores were assessed at 5, 15, and 60 min after delivery. Intraoperative vital signs and Apgar scores were compared using a linear mixed model and adjusted pairwise comparisons using Bonferroni analysis.Results: A total of 125 puppies were included in this study. Age, body weight, litter size, type of surgery, delivery time, anesthetic and surgical duration, and intraoperative vital signs did not significantly differ between the groups. Puppies in the alfaxalone and propofol groups had significantly higher Apgar scores than the etomidate group in both elective and emergency surgery. In elective surgery, Apgar scores at 5 min after delivery did not differ significantly between groups. At 15 and 60 min after delivery, Apgar scores in the etomidate group were significantly lower than those in the other groups. In emergency surgery, Apgar scores were significantly lower in the etomidate group than in the alfaxalone group at all time points.Conclusion: Induction with alfaxalone and propofol resulted in better outcomes with higher Apgar scores and neonatal viability than etomidate. Therefore, alfaxalone and propofol should be used as anesthetic induction drugs in both elective and emergency cesarean sections.Keywords: anesthesia, Apgar, puppies, viability, vigorous.
背景和目的:Apgar 评分是评估犬新生儿存活能力的有效方法。剖腹产幼犬的 Apgar 评分可能低于阴道分娩,因为所有麻醉药物都能穿过胎盘。因此,建议在剖腹产时使用对心肺功能影响最小且能快速排出的麻醉药物。本研究旨在比较依托咪酯、阿法沙隆或丙泊酚诱导后出生的幼犬与剖腹产时吸入异氟醚维持的幼犬的阿普加评分:将 36 只母羊平均分配到三种麻醉药物组。在分娩后 5、15 和 60 分钟评估改良阿普加评分。使用线性混合模型比较术中生命体征和 Apgar 评分,并使用 Bonferroni 分析调整配对比较:本研究共纳入 125 只幼犬。各组间的年龄、体重、产仔数、手术类型、分娩时间、麻醉和手术持续时间以及术中生命体征均无显著差异。在择期手术和急诊手术中,阿法沙龙组和丙泊酚组的幼犬阿普加评分明显高于依托咪酯组。在择期手术中,各组在分娩后 5 分钟的 Apgar 评分没有明显差异。在分娩后 15 分钟和 60 分钟,依托咪酯组的 Apgar 评分明显低于其他组别。在急诊手术中,依托咪酯组的Apgar评分在所有时间点都明显低于阿法沙龙组:结论:与依托咪酯相比,使用阿法沙龙和异丙酚诱导能获得更好的结果,Apgar评分更高,新生儿存活率更高。因此,阿法沙酮和丙泊酚应作为麻醉诱导药物用于择期和急诊剖宫产。
{"title":"Apgar scores in puppies following the induction of etomidate compared with alfaxalone or propofol for cesarean section","authors":"T. Srithunyarat, S. Jitpean, Piyasak Wipoosak, Chalermkwan Nonthakotr, N. Boonbal, P. Kunkitti, S. Seesupa","doi":"10.14202/vetworld.2024.527-534","DOIUrl":"https://doi.org/10.14202/vetworld.2024.527-534","url":null,"abstract":"Background and Aim: The Apgar score is a useful assessment of neonatal viability in dogs. The Apgar score in puppies born by cesarean section can be lower than vaginal delivery because all anesthetic drugs can cross the placenta. Therefore, anesthetic drugs with minimal cardiorespiratory effect and rapid elimination are recommended for cesarean section. The present study aimed to compare Apgar scores in puppies born after the induction of etomidate, alfaxalone or propofol, and those maintained with isoflurane inhalation during cesarean section.\u0000\u0000Materials and Methods: Thirty-six bitches were equally divided in the three anesthetic drug groups. Modified Apgar scores were assessed at 5, 15, and 60 min after delivery. Intraoperative vital signs and Apgar scores were compared using a linear mixed model and adjusted pairwise comparisons using Bonferroni analysis.\u0000\u0000Results: A total of 125 puppies were included in this study. Age, body weight, litter size, type of surgery, delivery time, anesthetic and surgical duration, and intraoperative vital signs did not significantly differ between the groups. Puppies in the alfaxalone and propofol groups had significantly higher Apgar scores than the etomidate group in both elective and emergency surgery. In elective surgery, Apgar scores at 5 min after delivery did not differ significantly between groups. At 15 and 60 min after delivery, Apgar scores in the etomidate group were significantly lower than those in the other groups. In emergency surgery, Apgar scores were significantly lower in the etomidate group than in the alfaxalone group at all time points.\u0000\u0000Conclusion: Induction with alfaxalone and propofol resulted in better outcomes with higher Apgar scores and neonatal viability than etomidate. Therefore, alfaxalone and propofol should be used as anesthetic induction drugs in both elective and emergency cesarean sections.\u0000\u0000Keywords: anesthesia, Apgar, puppies, viability, vigorous.","PeriodicalId":506834,"journal":{"name":"Veterinary World","volume":"35 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140276928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial effects of catnip (Nepeta cataria L.) essential oil against canine skin infection pathogens 猫薄荷(Nepeta cataria L.)精油对犬皮肤感染病原体的抗菌作用
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.585-592
G. Borlace, Ranee Singh, S. Seubsasana, Pranom Chantaranothai, Eakachai Thongkham, J. Aiemsaard
Background and Aim: Catnip essential oils have antimicrobial effects against bacteria, yeast, and fungi; however, there is limited information regarding their antimicrobial activity against pathogens that cause canine skin infections. This study aimed to identify the phytochemical constituents of catnip essential oil and assay its antimicrobial activity against Staphylococcus pseudintermedius, Malassezia pachydermatis, Microsporum canis, Microsporum gypseum, Microsporum gallinae, and Trichophyton mentagrophytes.Materials and Methods: Catnip essential oil was extracted by hydrodistillation, and its chemical constituents were analyzed by gas chromatography–mass spectrometry (GC–MS). In vitro antimicrobial activity was investigated using broth microdilution and time-kill tests. To evaluate the effect of catnip essential oil on microbial morphology and cell membrane integrity, scanning electron microscopy (SEM) and leakage studies were conducted.Results: GC–MS analysis revealed that the principal components of catnip essential oil were cis- and trans-nepetalactone (57.09% of peak area), trans-, cis-nepetalactone (39.69% of peak area), trans-caryophyllene (1.88% of peak area), and caryophyllene oxide (1.34% of peak area). The minimum inhibitory concentration, minimum bactericidal concentration, and minimum fungicidal concentration values determined by broth microdilution ranged from 0.0625 mg/mL to 4.0 mg/ mL. Time-kill testing showed that the germicidal effects of catnip essential oil were time and concentration-dependent, respectively. Environmental SEM and cell leakage analysis indicated that catnip essential oil disrupted the integrity of cell membranes in the tested microorganisms.Conclusion: Catnip essential oil has potential as an alternative antimicrobial against a wide range of canine skin infection pathogens, including S. pseudintermedius, M. pachydermatis, Mi. canis, Mi. gypseum, Mi. gallinae, and T. mentagrophytes.Keywords: antimicrobial activity, canine dermatitis, catnip, Nepeta cataria.
背景和目的:猫薄荷精油对细菌、酵母菌和真菌有抗菌作用,但有关其对引起犬皮肤感染的病原体的抗菌活性的信息却很有限。本研究旨在鉴定猫薄荷精油中的植物化学成分,并检测其对假中间葡萄球菌、马拉色菌、犬小孢子菌、石膏样小孢子菌、五倍子小孢子菌和毛癣菌的抗菌活性:猫薄荷精油采用水蒸馏法提取,其化学成分采用气相色谱-质谱法(GC-MS)进行分析。采用肉汤微稀释法和时间致死试验研究了猫薄荷精油的体外抗菌活性。为了评估猫薄荷精油对微生物形态和细胞膜完整性的影响,进行了扫描电子显微镜(SEM)和渗漏研究:气相色谱-质谱分析显示,猫薄荷精油的主要成分为顺式和反式庚内酯(占峰面积的 57.09%)、反式和顺式庚内酯(占峰面积的 39.69%)、反式茶碱(占峰面积的 1.88%)和氧化茶碱(占峰面积的 1.34%)。肉汤微稀释法测定的最低抑菌浓度、最低杀菌浓度和最低杀真菌浓度值介于 0.0625 毫克/毫升至 4.0 毫克/毫升之间。时间杀灭测试表明,猫薄荷精油的杀菌效果分别与时间和浓度有关。环境扫描电镜和细胞渗漏分析表明,猫薄荷精油破坏了受测微生物细胞膜的完整性:结论:猫薄荷精油具有替代抗菌剂的潜力,可用于抗击多种犬皮肤感染病原体,包括S. pseudintermedius、M. pachydermatis、Mi. canis、Mi. gypseum、Mi. gallinae和T. mentagrophytes。 关键词:抗菌活性;犬皮炎;猫薄荷;Nepeta cataria。
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引用次数: 0
Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility 精子活力正常和不良的巴厘牛体外胚胎发育能力比较
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.593-601
H. Hasbi, H. Iskandar, H. Sonjaya, B. Purwantara, R. I. Arifiantini, M. Agil, Berlin Pandapotan Pardede, S. Suyadi, W. A. Septian, Daud Samsudewa, Erni Damayanti, T. Maulana, Syahruddin Said
Background and Aim: Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts the reproductive rates. A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the quality of livestock. This study analyzed the developmental competence of in vitro embryos recovered from Bali cattle with normal or poor sperm motility.Materials and Methods: Nine bulls with normal fresh semen (NFS) or poor fresh semen (PFS) motility were ejaculated for semen. Semen ejaculates, including volume, motility, and sperm concentration, were evaluated immediately after collection to measure the quality of the fresh semen. Frozen semen was evaluated using computer-assisted semen analysis (CASA) for motility, progressive sperm motility, distance curve path, distance curve linear, distance straight line, average path velocity, curvilinear velocity, linear velocity, straightness (STR), linearity of forward progression (LIN), wobble, and average lateral head displacement (ALH). Bull groups were used to determine in vitro embryo cleavage ability after fertilization of Bali cattle. Ovaries of Bali cattle were collected by slicing, and only cytoplasmic oocytes with compact cumulus cells were used in this study. The oocytes were matured, and in vitro fertilization was performed using fertilization media with a final sperm concentration of 1.5 × 106 spermatozoa/mL. After 48 h, the embryo cleavage ability of the cultured oocytes was evaluated.Results: There were significant differences in motility values between the NFS and PFS groups; however, there were no significant differences in the volume or sperm concentration. There was a significant difference in the LIN value between the groups but no significant differences in other CASA parameters. There were no significant differences in the cleavage rate and morula between the groups, but a positive correlation was observed between the cleavage rate and the morula and between the morula and ALH. A significant negative correlation was observed between the cleavage rate and STR and between the morula and STR; no significant differences were observed for other variables.Conclusion: Despite variations in sperm characteristics, both normal and poor sperm motility demonstrated comparable in vitro embryonic development competence. These findings provide important insights into the fertility potential of Bali bulls, providing valuable information that can enhance selection strategies to improve the quality of livestock production.Keywords: Bali cattle, embryos, in vitro semen quality.
背景和目的:生育力直接影响繁殖率,因此对提高畜牧业生产效率至关重要。全面了解精子质量与胚胎发育之间的关系是优化繁殖结果和提高家畜质量的关键。本研究分析了精子活力正常或不良的巴厘牛体外胚胎的发育能力:对 9 头新鲜精液(NFS)活力正常或新鲜精液(PFS)活力差的公牛进行精液射精。采集精液后立即对精液量、活力和精子浓度进行评估,以衡量新鲜精液的质量。使用计算机辅助精液分析(CASA)对冷冻精液的运动性、精子渐进运动性、曲线路径距离、曲线直线距离、直线距离、平均路径速度、曲线速度、直线速度、直线度(STR)、前向直线度(LIN)、摆动和平均侧头位移(ALH)进行评估。公牛组用于测定巴厘牛受精后的体外胚胎裂解能力。通过切片采集巴厘岛牛的卵巢,本研究仅使用具有紧凑积层细胞的胞质卵母细胞。卵母细胞成熟后,使用最终精子浓度为 1.5 Ã- 106 个精子/毫升的受精培养基进行体外受精。48 h后,评估培养卵母细胞的胚胎裂解能力:NFS组和PFS组的活力值有明显差异,但精子量和精子浓度无明显差异。两组的 LIN 值有显著差异,但其他 CASA 参数无显著差异。各组之间的卵裂率和畸形率无明显差异,但卵裂率与畸形率之间以及畸形率与 ALH 之间呈正相关。裂解率与STR之间以及畸形率与STR之间呈明显的负相关;其他变量之间无明显差异:结论:尽管精子的特征存在差异,但正常和不良精子的体外胚胎发育能力相当。这些发现为了解巴厘公牛的繁殖潜力提供了重要依据,为改进选育策略、提高畜牧业生产质量提供了宝贵信息:巴厘牛 胚胎 体外精液质量
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引用次数: 0
Nutrient digestibility, characteristics of rumen fermentation, and microbial protein synthesis from Pesisir cattle diet containing non-fiber carbohydrate to rumen degradable protein ratio and sulfur supplement 含有非纤维碳水化合物与瘤胃可降解蛋白质比率和硫补充剂的 Pesisir 牛日粮的营养消化率、瘤胃发酵特征和微生物蛋白质合成
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.672-681
M. Zain, U. H. Tanuwiria, J. A. Syamsu, Yunilas Yunilas, R. Pazla, E. M. Putri, M. Makmur, Ummi Amanah, Putri Okta Shafura, Bima Bagaskara
Background and Aim: To achieve optimal feed efficiency in ruminants, especially Pesisir cattle, it is necessary to maintain a harmonious equilibrium between energy and protein levels within the rumen. Sulfur supplementation can potentially escalate the energy–protein balance in the rumen. The aim of this study was to explore the formulation of ruminant diets by synchronizing rumen degradable protein (RDP) and non-fiber carbohydrate (NFC) while adding sulfur minerals at different levels. Nutrient digestibility, NH3 concentration, volatile fatty acids (VFA) production, microbial protein synthesis (MPS), and methane gas production were assessed.Materials and Methods: We employed a randomized block design with a 2 × 2 × 3 factorial arrangement and examined diverse incubation periods of 6, 24, and 48 h. Treatment consisted of RDP (60% and 65%), NFC (35% and 40%), and sulfur (0%, 0.15%, and 0.3%) levels. In this study, the Tilley and Terry in vitro technique, which used Pesisir cattle’s rumen fluid, was employed to assess the digestibility of dry matter, organic matter, acid detergent fiber, neutral detergent fiber, and RDP-Rumen undegradable protein. In addition, it measures various rumen fluid attributes, including pH, NH3, VFA, MPS, and methane gas production.Results: Treatment with a coordinated combination of 65% RDP and 40% NFC combined with 0.15% sulfur supplement yielded significantly improved digestibility and notably reduced methane gas production (p < 0.05).Conclusion: The enhancement in digestibility and reduction in methane gas emissions can be attributed to the interaction of RDP, NFC, and sulfur. Feed digestibility was increased in the 65% RDP treatment with 40% NFC and 0.15% sulfur, along with a decrease in methane gas production.Keywords: degradable and undegradable protein, digestibility, non-fiber carbohydrate, rumen fermentation, sulfur.
背景和目的:为了使反刍动物(尤其是 Pesisir 牛)获得最佳饲料效率,有必要保持瘤胃内能量和蛋白质水平的和谐平衡。补硫可能会加剧瘤胃中的能量-蛋白质平衡。本研究的目的是通过同步瘤胃可降解蛋白质(RDP)和非纤维碳水化合物(NFC),同时添加不同水平的硫矿物质,探索反刍动物日粮的配方。对营养物质消化率、NH3 浓度、挥发性脂肪酸(VFA)产量、微生物蛋白质合成(MPS)和甲烷气体产量进行了评估:处理包括 RDP(60% 和 65%)、NFC(35% 和 40%)和硫(0%、0.15% 和 0.3%)水平。本研究采用 Tilley 和 Terry 体外技术,使用 Pesisir 牛的瘤胃液来评估干物质、有机物质、酸性洗涤纤维、中性洗涤纤维和 RDP-Rumen 难降解蛋白质的消化率。此外,它还能测量瘤胃液的各种属性,包括 pH 值、NH3、VFA、MPS 和甲烷气体产量:使用 65% RDP 和 40% NFC 的协调组合以及 0.15% 的硫补充剂进行处理,可显著提高消化率并明显减少甲烷气体的产生(p < 0.05):消化率的提高和甲烷气体排放量的减少可归因于 RDP、NFC 和硫的相互作用。可降解和不可降解蛋白质、消化率、非纤维碳水化合物、瘤胃发酵、硫。
{"title":"Nutrient digestibility, characteristics of rumen fermentation, and microbial protein synthesis from Pesisir cattle diet containing non-fiber carbohydrate to rumen degradable protein ratio and sulfur supplement","authors":"M. Zain, U. H. Tanuwiria, J. A. Syamsu, Yunilas Yunilas, R. Pazla, E. M. Putri, M. Makmur, Ummi Amanah, Putri Okta Shafura, Bima Bagaskara","doi":"10.14202/vetworld.2024.672-681","DOIUrl":"https://doi.org/10.14202/vetworld.2024.672-681","url":null,"abstract":"Background and Aim: To achieve optimal feed efficiency in ruminants, especially Pesisir cattle, it is necessary to maintain a harmonious equilibrium between energy and protein levels within the rumen. Sulfur supplementation can potentially escalate the energy–protein balance in the rumen. The aim of this study was to explore the formulation of ruminant diets by synchronizing rumen degradable protein (RDP) and non-fiber carbohydrate (NFC) while adding sulfur minerals at different levels. Nutrient digestibility, NH3 concentration, volatile fatty acids (VFA) production, microbial protein synthesis (MPS), and methane gas production were assessed.\u0000\u0000Materials and Methods: We employed a randomized block design with a 2 × 2 × 3 factorial arrangement and examined diverse incubation periods of 6, 24, and 48 h. Treatment consisted of RDP (60% and 65%), NFC (35% and 40%), and sulfur (0%, 0.15%, and 0.3%) levels. In this study, the Tilley and Terry in vitro technique, which used Pesisir cattle’s rumen fluid, was employed to assess the digestibility of dry matter, organic matter, acid detergent fiber, neutral detergent fiber, and RDP-Rumen undegradable protein. In addition, it measures various rumen fluid attributes, including pH, NH3, VFA, MPS, and methane gas production.\u0000\u0000Results: Treatment with a coordinated combination of 65% RDP and 40% NFC combined with 0.15% sulfur supplement yielded significantly improved digestibility and notably reduced methane gas production (p < 0.05).\u0000\u0000Conclusion: The enhancement in digestibility and reduction in methane gas emissions can be attributed to the interaction of RDP, NFC, and sulfur. Feed digestibility was increased in the 65% RDP treatment with 40% NFC and 0.15% sulfur, along with a decrease in methane gas production.\u0000\u0000Keywords: degradable and undegradable protein, digestibility, non-fiber carbohydrate, rumen fermentation, sulfur.","PeriodicalId":506834,"journal":{"name":"Veterinary World","volume":"159 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140270786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of temperature on the virulence of Streptococcus agalactiae in Indonesian aquaculture: A better vaccine design is required 温度对印度尼西亚水产养殖中无乳酸链球菌毒力的影响:需要更好的疫苗设计
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.682-689
A. Lusiastuti, A. Suhermanto, Bernadetta Rina Hastilestari, Suryanto Suryanto, Mira Mawardi, D. Sugiani, Dewi Syahidah, P. E. Sudaryatma, Domenico Caruso
Due to their poikilothermic nature, fish are very sensitive to changes in temperature. Due to climate change, the average global temperature has increased by 1.5°C in the last century, which may have caused an increase in farmed fish mortality recently. Predictions using the model estimate that a 1°C increase in temperature could cause 3%-4% and 4%-6% mortality due to infectious diseases in organisms living in warm and temperate waters, respectively. There is a need to determine whether there is a relationship between increasing environmental temperature and disease virulence. This review examines the influence and impact of increasing temperatures due to climate change on the physiology and pathogenicity of Streptococcus agalactiae, which causes streptococcosis in tilapia and causes significant economic losses. Changes in the pathogenicity of S. agalactiae, especially its virulence properties due to increasing temperature, require changes in the composition design of the fish vaccine formula to provide better protection through the production of protective antibodies.Keywords: adaptation, microbes, pathogen, temperature, virulence.
由于鱼类具有低温特性,它们对温度变化非常敏感。由于气候变化,上个世纪全球平均气温上升了 1.5°C,这可能导致最近养殖鱼类死亡率上升。根据模型预测估计,气温上升 1°C 可分别导致生活在温暖水域和温带水域的生物因传染病死亡 3%-4%和 4%-6%。有必要确定环境温度升高与疾病毒性之间是否存在关系。本综述探讨了气候变化导致气温升高对导致罗非鱼链球菌病并造成重大经济损失的无乳链球菌的生理和致病性的影响和冲击。随着温度的升高,S. agalactiae 的致病性发生变化,尤其是其毒力特性的变化,需要改变鱼类疫苗配方的组成设计,通过产生保护性抗体来提供更好的保护。
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引用次数: 0
Physicochemical properties and fatty acid profile of oil extracted from black soldier fly larvae (Hermetia illucens) 从黑兵器蝇幼虫(Hermetia illucens)中提取的油的理化性质和脂肪酸概况
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.518-526
Krittika Srisuksai, Paviga Limudomporn, U. Kovitvadhi, Khunakon Thongsuwan, Witcha Imaram, Ratchaphon Lertchaiyongphanit, Tharinee Sareepoch, A. Kovitvadhi, W. Fungfuang
Background and Aim: Hermetia illucens, a black soldier fly, is widely recognized for sustainable recycling of organic waste. Black soldier fly larvae (BSFLs) can consume various types of biowastes and convert them into nutrient-rich biomass, including proteins, lipids, chitin, and minerals. This study investigated the best extraction method by comparing the fatty acid profiles, percentage yield, and antioxidant properties of BSFL oil extracted using different extraction methods.Materials and Methods: The physicochemical properties, fatty acid profile, and free radical scavenging ability of BSFL oil were analyzed using six extraction methods.Results: Ultrasonic extraction with hexane resulted in the highest yields compared with different extraction methods. Lauric acid (28%–37%) was the most abundant fatty acid in all extracts, followed by palmitic acid, myristic acid, oleic acid, and linoleic acid. Compared with other methods, aqueous extraction showed the highest lauric acid composition and free radical scavenging activities. In addition, high-temperature aqueous extraction resulted in higher oil yield and free radical scavenging activities than low-temperature extraction.Conclusion: High-temperature aqueous extraction is the best extraction method because it is rich in lauric acid, has antioxidant ability, and can be further developed to produce novel sustainable biomaterials for humans and animals.Keywords: black soldier fly, extraction method, fatty acid, Hermetia illucens.
背景和目的:黑兵蝇(Hermetia illucens)被广泛认为是一种可持续回收利用有机废物的害虫。黑翅大实蝇幼虫(BSFLs)可食用各种类型的生物废物,并将其转化为营养丰富的生物质,包括蛋白质、脂类、甲壳素和矿物质。本研究通过比较不同萃取方法提取的 BSFL 油的脂肪酸谱、产量百分比和抗氧化特性,研究最佳萃取方法:采用六种萃取方法分析了 BSFL 油的理化性质、脂肪酸谱和自由基清除能力:结果:与不同的萃取方法相比,用正己烷超声波萃取的产量最高。月桂酸(28%-37%)是所有提取物中含量最高的脂肪酸,其次是棕榈酸、肉豆蔻酸、油酸和亚油酸。与其他方法相比,水提取法的月桂酸成分和自由基清除活性最高。此外,与低温萃取相比,高温水萃取的出油率和自由基清除活性更高:结论:高温水萃取是最佳的萃取方法,因为它富含月桂酸,具有抗氧化能力,可进一步开发用于生产新型可持续生物材料,供人类和动物使用。 关键词:黑兵蝇;萃取方法;脂肪酸;Hermetia illucens。
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引用次数: 0
Current trends and spatial-temporal dynamics of veterinary dentistry research: A scientometric study 兽医牙科研究的当前趋势和时空动态:科学计量学研究
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.666-671
Daniel Alvitez-Temoche, Elca del Aguila, Diego Ga, larza-Valencia, Ivan Calderon, Fran Espinoza-Carhuancho, Josmel Pacheco-Mendoza, Frank Mayta-Tovalino
Background and Aim: Understanding dental care in dogs has made remarkable progress in veterinary medicine. Therefore, this study aimed to analyze the academic literature published in veterinary dentistry from 1990 to 2023.Materials and Methods: A descriptive study was conducted using a scientometric approach and metadata from the Web of Science database. A search strategy adapted for this database was developed using MeSH and Emtree terms and the Boolean operators AND and OR. Using Bibliometrix, different metrics were evaluated to assess the scientific production of researchers and institutions and the impact of authors based on their publications. CiteSpace was also used for co-citation analysis and visualization of citation networks, trends, and patterns in this field of study over time.Results: The bibliometric study analyzed 211 documents from 50 different sources from 1990 to 2023, with an annual growth rate of 6.5%, covering the period 1990–2023. A total of 474 authors were identified, with an average of 2.82 coauthors per paper and 11.85% international coauthorships. The average age of the papers was 12.4 years and 4.55 citations per paper. The most common types of documents were articles (154 documents).Conclusion: Research in veterinary dentistry has shown steady growth from 1990 to 2023. Although there have been fluctuations in article production over the years, there has been a steady growth in article production in veterinary dentistry in general. The annual average number of citations per article has varied over the years, reaching 45 in 2015. However, the average number of citations per article has decreased significantly from 2021 to 2023.Keywords: bibliometrix, scientometrics, veterinary dentistry.
背景和目的:在兽医学领域,了解犬的牙科护理已取得显著进展。因此,本研究旨在分析 1990 年至 2023 年期间发表的兽医牙科学术文献:本研究采用科学计量学方法和科学网数据库中的元数据进行描述性研究。使用 MeSH 和 Emtree 术语以及布尔运算符 AND 和 OR 制定了适合该数据库的搜索策略。使用 Bibliometrix 评估了不同的指标,以评估研究人员和机构的科研成果以及作者根据其出版物产生的影响。此外,还使用 CiteSpace 进行了共引分析,并对该研究领域长期以来的引文网络、趋势和模式进行了可视化分析:文献计量学研究分析了 1990 年至 2023 年期间来自 50 个不同来源的 211 篇文献,年增长率为 6.5%。共确定了 474 位作者,平均每篇论文有 2.82 位共同作者,国际共同作者占 11.85%。论文的平均年龄为 12.4 年,每篇论文的引用次数为 4.55 次。最常见的文献类型是文章(154 篇):从 1990 年到 2023 年,兽医牙科的研究一直在稳步增长。虽然多年来文章产量有所波动,但总体而言,兽医牙科学的文章产量一直在稳步增长。每篇文章的年均被引次数多年来有所变化,2015 年达到 45 次。然而,从2021年到2023年,每篇文章的平均被引次数明显下降。关键词:文献计量学;科学计量学;兽医牙科学。
{"title":"Current trends and spatial-temporal dynamics of veterinary dentistry research: A scientometric study","authors":"Daniel Alvitez-Temoche, Elca del Aguila, Diego Ga, larza-Valencia, Ivan Calderon, Fran Espinoza-Carhuancho, Josmel Pacheco-Mendoza, Frank Mayta-Tovalino","doi":"10.14202/vetworld.2024.666-671","DOIUrl":"https://doi.org/10.14202/vetworld.2024.666-671","url":null,"abstract":"Background and Aim: Understanding dental care in dogs has made remarkable progress in veterinary medicine. Therefore, this study aimed to analyze the academic literature published in veterinary dentistry from 1990 to 2023.\u0000\u0000Materials and Methods: A descriptive study was conducted using a scientometric approach and metadata from the Web of Science database. A search strategy adapted for this database was developed using MeSH and Emtree terms and the Boolean operators AND and OR. Using Bibliometrix, different metrics were evaluated to assess the scientific production of researchers and institutions and the impact of authors based on their publications. CiteSpace was also used for co-citation analysis and visualization of citation networks, trends, and patterns in this field of study over time.\u0000\u0000Results: The bibliometric study analyzed 211 documents from 50 different sources from 1990 to 2023, with an annual growth rate of 6.5%, covering the period 1990–2023. A total of 474 authors were identified, with an average of 2.82 coauthors per paper and 11.85% international coauthorships. The average age of the papers was 12.4 years and 4.55 citations per paper. The most common types of documents were articles (154 documents).\u0000\u0000Conclusion: Research in veterinary dentistry has shown steady growth from 1990 to 2023. Although there have been fluctuations in article production over the years, there has been a steady growth in article production in veterinary dentistry in general. The annual average number of citations per article has varied over the years, reaching 45 in 2015. However, the average number of citations per article has decreased significantly from 2021 to 2023.\u0000\u0000Keywords: bibliometrix, scientometrics, veterinary dentistry.","PeriodicalId":506834,"journal":{"name":"Veterinary World","volume":"14 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140268582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Veterinary World
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