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Assessment of knowledge, attitudes, and practices related to the prevalence of sheep scab among communal sheep farmers in Eastern Cape Province, South Africa 评估南非东开普省公社养羊户对绵羊疥疮发病率的认识、态度和做法
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.558-563
M. Yawa, Bukeka Mtenjwa, I. Jaja, S. Mthi, N. Nyangiwe, S. Tokozwayo, T. Stempa, L. Qokweni
Background and Aim: Sheep scab is one of the most contagious diseases of sheep found in rural communities worldwide and is a major health and welfare concern for sheep farming. Information on the attitudes of communal farmers to sheep scab remains speculative in the Eastern Cape Province. This study aimed to investigate knowledge, attitudes, and practices related to the prevalence of sheep scab among communal sheep farmers in Eastern Cape Province, South Africa.Materials and Methods: From June to August 2022, a cross-sectional survey using a semi-structured questionnaire (n = 160) was conducted in two rural communities of the Enoch Mgijima Local Municipality in Eastern Cape Province. Purpose sampling was used to obtain respondents’ knowledge, attitudes, and management practices regarding sheep scabs.Results: Among respondents, 81% were males and 19% were females. The majority of sheep farmers (59%) in both communities were aware of sheep scabs, but the majority (64%) did not receive any farming training. The majority of sheep farmers (65%) believed that sheep scab was the most serious threat to their sheep production since it increased mortality. In the past 3 years, approximately 57% of respondents reported an increase in the prevalence of sheep scab. The majority of farmers reported wool loss as the most noticeable clinical symptom (73%). More than 80% of sheep farmers do not use acaricide on a regular basis. Sixty-one percent of farmers prefer a pour-on topical application method. The frequency of annual dipping was every fortnight (40%), during summer and winter (24%). Most farmers (66%) regularly vaccinate their sheep.Conclusion: The prevalence of sheep scab is increasing in these communities; however, a better understanding of the factors that facilitate its transmission is required to allow improved management and control of this disease. The government must involve extension officers and distribute them to disseminate information to farmers. Thus, it will be easy to translate the literature into action.Keywords: communal sheep farmers, Eastern Cape Province, season, sheep scab control methods, sheep scab.
背景和目的:绵羊疮痂病是全世界农村地区发现的最具传染性的绵羊疾病之一,也是养羊业的一个主要健康和福利问题。在东开普省,有关乡镇农民对羊疥癣的态度的信息仍然是猜测性的。本研究旨在调查南非东开普省公社养羊户对绵羊疥疮流行的相关知识、态度和做法:2022 年 6 月至 8 月,在东开普省 Enoch Mgijima 地方市的两个农村社区使用半结构式问卷(n = 160)进行了横断面调查。调查采用目的抽样法,以了解受访者对羊疥癣的认识、态度和管理方法:在受访者中,81% 为男性,19% 为女性。两个社区的大多数养羊人(59%)都知道羊疥癣,但大多数(64%)没有接受过任何养殖培训。大多数养羊户(65%)认为羊疥癣是对其羊群生产最严重的威胁,因为羊疥癣会增加死亡率。在过去 3 年中,约有 57% 的受访者表示羊疥癣的发病率有所上升。大多数养殖户称羊毛脱落是最明显的临床症状(73%)。超过 80% 的养羊户没有定期使用杀螨剂。61%的养殖户喜欢使用浇灌式局部施药方法。每年浸药的频率为每两周一次(40%),夏季和冬季各一次(24%)。大多数农民(66%)定期给羊接种疫苗:结论:绵羊疮痂病在这些社区的流行率正在上升;然而,需要更好地了解促进其传播的因素,以便改进对这种疾病的管理和控制。政府必须让推广人员参与进来,让他们向农民传播信息。关键词:社区养羊户、东开普省、季节、羊疮痂病防治方法、羊疮痂病。
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引用次数: 0
Seroprevalence of peste des petits ruminants disease in Indonesian buffaloes may be an emerging threat to small ruminants 印度尼西亚水牛的小反刍兽疫血清流行率可能是对小型反刍动物的新威胁
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.535-539
I. Sendow, Heri Hoerudin, Risza Hartawan, Nuha Fairusya, A. Ratnawati, A. H. Wardhana, D. Sawitri, H. Nuradji, N. Dharmayanti, M. Saepulloh, E. Chowdhury
Background and Aim: The peste des petit ruminants (PPR) is a disaster-class virus that causes catastrophic drawbacks to small ruminant industries in affected countries. As PPR disease has been reported in neighboring countries, Indonesia, which has a large population of sheep and goats, has become prone to the emerging threat of infection. Because the virus can also infect other animals with subclinical manifestations, large ruminants, such as buffaloes, may play an important role in spreading the virus in the environment. Therefore, the main objective of this study was to identify PPR seroprevalence in the buffalo population of Indonesia.Materials and Methods: A competitive enzyme-linked immunosorbent assay was performed to identify the specific antibody for PPR viruses in the buffalo population using serum bank collection from the National Research and Innovation Agency, Indonesia.Results: PPR virus seroprevalence was detected in buffalo from Central Java, East Java, and East Nusa Tenggara Province in Indonesia. Although seroprevalence was low in the population, the antibody titer was relatively high in the positive samples. Sex and age were identified as determinant factors in the seroprevalence distribution of the buffalo population.Conclusion: The presence of antibodies against the PPR virus in buffaloes may indicate that PPR virus is circulating in the buffalo population of Indonesia.Keywords: antibody, buffalo, Indonesia, peste des petit ruminants, seroprevalence.
背景与目的:小反刍兽疫(PPR)是一种灾难级病毒,会给受影响国家的小反刍兽疫产业带来灾难性的后果。随着周边国家相继出现 PPR 疾病的报道,拥有大量绵羊和山羊的印度尼西亚也很容易受到新出现的感染威胁。由于该病毒还能以亚临床表现感染其他动物,水牛等大型反刍动物可能在环境中传播病毒方面扮演重要角色。因此,本研究的主要目的是确定印度尼西亚水牛群体中的 PPR 血清流行率:采用竞争性酶联免疫吸附试验,利用从印度尼西亚国家研究与创新局血清库中采集的血清,鉴定水牛群体中 PPR 病毒的特异性抗体:结果:在印度尼西亚中爪哇省、东爪哇省和东努沙登加拉省的水牛中检测到了 PPR 病毒血清阳性反应。虽然血清流行率较低,但阳性样本的抗体滴度相对较高。性别和年龄是影响水牛血清流行率分布的决定性因素:关键词:抗体;水牛;印度尼西亚;小反刍兽疫;血清流行率。
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引用次数: 0
Seroprevalence and risk factors associated with Theileria parva infection among calves in Narok County, Kenya 肯尼亚纳罗克县小牛感染副丝虫的血清流行率和相关风险因素
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.620-629
Wyckliff Ngetich, G. Gitau, T. Okumu, G. Aboge, Daniel Muasya
Background and Aim: East Coast fever (ECF), caused by Theileria parva, is a devastating disease that causes significant economic losses to cattle production in sub-Saharan Africa. Prevention and control of ECF are challenging in pastoral settings due to inadequate epidemiological information. This study aimed to estimate the seroprevalence and risk factors associated with T. parva infection among calves in different production systems to help design appropriate control interventions.Materials and Methods: Blood samples were collected from 318 calves and tested using an indirect enzyme-linked immunosorbent assay targeting antibodies against polymorphic immunodominant molecules found on the surface of T. parva. Information on calf characteristics and management practices was also collected during sampling. Descriptive statistics and logistic regression were used to analyze potential risk factors, such as age and acaricide application, where p < 0.05 was considered significantResults: Of the 318 calves sampled, 41 (12.89%) were positive for T. parva, with a higher proportion in pastoral systems (36.58%) than in mixed farming systems (34.10%) and agropastoral systems (29.27%). From univariate analysis, calf age (p = 0.002), body weight (p = 0.001), suckling status (p = 0.026), rectal temperature (p = 0.06), calves on pasture (p = 0.022), other feeds (p = 0.004), feed grown within the farm (p = 0.004), acaricide application (p = 0.001), and acaricide application frequency (p = 0.001) were significantly associated with seropositivity. However, calf age (odds ratio [OR], 0.96; 95% confidence interval [CI], 0.91-0.99; p = 0.04), other feeds (OR, 8.82; 95% CI, 1.74-44.63; p = 0.009), and suckling status (OR, 0.38; 95% CI, 0.15-0.99; p = 0.05) were significantly associated with T. parva infection in the multivariable mixed logistic model.Conclusion: T. parva is circulating in young calves in the study area (and possibly in cattle populations due to maternal transfer of antibodies to the calves). There is a need for molecular surveillance to determine the presence and burden of T. parva infection.Keywords: calves, risk factors, seroprevalence, Theileria parva.
背景与目的:由副丝虫引起的东海岸热(ECF)是一种毁灭性疾病,给撒哈拉以南非洲地区的牛群生产造成了重大经济损失。由于流行病学信息不足,在牧区预防和控制东海岸热具有挑战性。本研究旨在估算不同生产系统中犊牛的血清流行率以及与T. parva感染相关的风险因素,以帮助设计适当的控制干预措施:收集了 318 头犊牛的血样,并使用间接酶联免疫吸附试验进行检测,该试验的目标抗体是针对疟原虫表面的多态免疫显性分子。采样期间还收集了犊牛特征和管理方法的信息。使用描述性统计和逻辑回归分析潜在的风险因素,如年龄和杀螨剂的使用,P < 0.05 为显著结果:在采样的 318 头犊牛中,有 41 头(12.89%)对副疟原虫呈阳性反应,牧区的比例(36.58%)高于混养区(34.10%)和农牧区(29.27%)。单变量分析显示,犊牛年龄(p = 0.002)、体重(p = 0.001)、哺乳状况(p = 0.026)、直肠温度(p = 0.06)、牧草上的犊牛(p = 0.022)、其他饲料(p = 0.004)、农场内种植的饲料(p = 0.004)、杀螨剂施用(p = 0.001)和杀螨剂施用频率(p = 0.001)与血清阳性率显著相关。然而,在多变量混合逻辑模型中,犊牛年龄(几率比[OR],0.96;95% 置信区间[CI],0.91-0.99;p = 0.04)、其他饲料(OR,8.82;95% CI,1.74-44.63;p = 0.009)和哺乳状况(OR,0.38;95% CI,0.15-0.99;p = 0.05)与T. parva感染明显相关:结论:T. parva 在研究地区的幼犊中流行(也可能因母体将抗体传给幼犊而在牛群中流行)。关键词:犊牛;风险因素;血清流行率;副猪嗜血杆菌。
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引用次数: 0
A pilot study on the pulmonary anthracosis in stray dogs of Kathmandu Valley, Nepal: A potential public health threat for future 关于尼泊尔加德满都谷地流浪狗肺炭疽病的试点研究:对未来公共卫生的潜在威胁
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.658-665
Sunil Thapa, Rajesh Bhatta, Bikash Puri, R. Bashyal, Romi Kunwar, Swochhal Prakash Shrestha, Girija Regmi, Pushkar Pal
Background and Aim: Kathmandu is a densely populated metropolitan city in Nepal. In recent years, however, the metropolis has been ranked as one of the most polluted cities worldwide. Both humans and animals are susceptible to various respiratory diseases due to chronic exposure to polluted air. Due to the relative similarities in the anatomical structure and physiological functions of the respiratory system between humans and dogs, polluted environments may lead to respiratory illness in similar ways in both species living in the valley. On the basis of information on the air quality in the valley, this study was conceived to assess pulmonary illness in street dogs to discern the health hazards caused by polluted air.Materials and Methods: A total of 76 dogs with clinical signs of tachypnea, dyspnea, sneezing, coughing, mucopurulent discharge, moderate hyperthermia, and anorexia admitted from July 2020 to November 2020 in Animal Nepal for treatment were included in this study. Among them, 24 animals responded to treatment, and 52 dogs died during their stay in the hospital. The 52 dead animals were necropsied, and the lesions that resembled pulmonary anthracosis were further studied grossly and histologically in a blinded fashion by trained veterinary pathologists.Results: Significant morphological alterations were observed in the lungs and associated lymph nodes of 25 animals, indicating pulmonary anthracosis. Gross morphological changes included multiple black foci with hemorrhage, congestion, nodular, and emphysema on the parietal and visceral surfaces of the lungs. The alveolar septa and visceral pleura exhibited deposition of black particles. Congestion, emphysema, and inflammatory exudates were also detected in the lung tissues and lymph nodes.Conclusion: The clinical, gross, and microscopic findings accurately resembled those of pulmonary anthracosis. This life-threatening condition in stray canines may be caused by a critical level of air pollution from different sources and carbon emissions from vehicles. To protect animals and humans living in the Kathmandu Valley, concerned government and non-government agencies should work toward reducing air pollution levels as soon as possible.Keywords: air pollution, histopathology, Kathmandu, pulmonary anthracosis, respiratory disease, stray dogs.
背景和目的:加德满都是尼泊尔人口稠密的大都市。然而,近年来,这座大都市已被列为全球污染最严重的城市之一。由于长期暴露在污染的空气中,人类和动物都很容易患上各种呼吸道疾病。由于人和狗的呼吸系统在解剖结构和生理功能上相对相似,污染环境可能会以类似的方式导致生活在山谷中的两种动物患上呼吸系统疾病。根据山谷空气质量的相关信息,本研究旨在评估流浪狗的肺部疾病,以发现污染空气对健康的危害:本研究共纳入了从 2020 年 7 月至 2020 年 11 月在尼泊尔动物园接受治疗的 76 只临床表现为呼吸急促、呼吸困难、打喷嚏、咳嗽、粘液脓性分泌物、中度高热和厌食的狗。其中,24 只动物接受了治疗,52 只狗在住院期间死亡。对这52只死亡动物进行了尸体解剖,并由训练有素的兽医病理学家在盲法下对类似肺炭疽的病变进行了进一步的大体和组织学研究:结果:在 25 只动物的肺部和相关淋巴结中观察到了明显的形态变化,表明患上了肺炭疽病。大体形态变化包括肺顶叶和内脏表面出现多个黑色病灶,伴有出血、充血、结节和气肿。肺泡间隔和内脏胸膜有黑色颗粒沉积。肺组织和淋巴结中也发现了充血、气肿和炎性渗出物:结论:临床、大体和显微镜检查结果都与肺炭疽病十分相似。流浪犬的这种危及生命的病症可能是由不同来源的空气污染和车辆的碳排放造成的。为了保护生活在加德满都谷地的动物和人类,相关政府和非政府机构应尽快降低空气污染水平。关键词:空气污染;组织病理学;加德满都;肺炭疽病;呼吸系统疾病;流浪狗。
{"title":"A pilot study on the pulmonary anthracosis in stray dogs of Kathmandu Valley, Nepal: A potential public health threat for future","authors":"Sunil Thapa, Rajesh Bhatta, Bikash Puri, R. Bashyal, Romi Kunwar, Swochhal Prakash Shrestha, Girija Regmi, Pushkar Pal","doi":"10.14202/vetworld.2024.658-665","DOIUrl":"https://doi.org/10.14202/vetworld.2024.658-665","url":null,"abstract":"Background and Aim: Kathmandu is a densely populated metropolitan city in Nepal. In recent years, however, the metropolis has been ranked as one of the most polluted cities worldwide. Both humans and animals are susceptible to various respiratory diseases due to chronic exposure to polluted air. Due to the relative similarities in the anatomical structure and physiological functions of the respiratory system between humans and dogs, polluted environments may lead to respiratory illness in similar ways in both species living in the valley. On the basis of information on the air quality in the valley, this study was conceived to assess pulmonary illness in street dogs to discern the health hazards caused by polluted air.\u0000\u0000Materials and Methods: A total of 76 dogs with clinical signs of tachypnea, dyspnea, sneezing, coughing, mucopurulent discharge, moderate hyperthermia, and anorexia admitted from July 2020 to November 2020 in Animal Nepal for treatment were included in this study. Among them, 24 animals responded to treatment, and 52 dogs died during their stay in the hospital. The 52 dead animals were necropsied, and the lesions that resembled pulmonary anthracosis were further studied grossly and histologically in a blinded fashion by trained veterinary pathologists.\u0000\u0000Results: Significant morphological alterations were observed in the lungs and associated lymph nodes of 25 animals, indicating pulmonary anthracosis. Gross morphological changes included multiple black foci with hemorrhage, congestion, nodular, and emphysema on the parietal and visceral surfaces of the lungs. The alveolar septa and visceral pleura exhibited deposition of black particles. Congestion, emphysema, and inflammatory exudates were also detected in the lung tissues and lymph nodes.\u0000\u0000Conclusion: The clinical, gross, and microscopic findings accurately resembled those of pulmonary anthracosis. This life-threatening condition in stray canines may be caused by a critical level of air pollution from different sources and carbon emissions from vehicles. To protect animals and humans living in the Kathmandu Valley, concerned government and non-government agencies should work toward reducing air pollution levels as soon as possible.\u0000\u0000Keywords: air pollution, histopathology, Kathmandu, pulmonary anthracosis, respiratory disease, stray dogs.","PeriodicalId":506834,"journal":{"name":"Veterinary World","volume":"34 30","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140276945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative analysis of post-operative rehabilitation approaches for medial patellar luxation in small-breed dogs 小型犬髌骨内侧脱位术后康复方法比较分析
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.550-557
E. Akaraphutiporn, Irin Kwananocha, Chularach Meechai, Pijitra Suksomboonwong, Suchanun Aramsriprasert, Ornjira Meethong, Arttapol Triampitak, C. Wangdee
Background and Aim: Electrical stimulation (ES) and light amplification by stimulated emission of radiation (LASER) therapy are frequently used in post-operative rehabilitation; however, there is currently insufficient research comparing their effectiveness. This study aimed to assess the effectiveness of post-operative rehabilitation following medial patellar luxation (MPL) surgical correction by comparing ES and LASER therapy when combined with exercise. This was compared with a control group that consisted solely of post-operative home exercise implemented by the owner.Materials and Methods: We conducted a prospective clinical trial on dogs that had undergone surgical treatment for MPL. The dogs were categorized into the following three groups: The control group, which did not participate in any post-operative rehabilitation program; the ES group, which received post-operative rehabilitation involving ES therapy; and the LASER group, which underwent post-operative rehabilitation featuring LASER therapy.Results: There were no significant differences among the groups regarding the evaluation parameters, including lameness score, pain score, thigh muscle circumference, and range of motion. Although there may have been a difference in pain score in some groups, it could be attributed to the pre-operative condition of patients. These results aligned with the owner questionnaires’ canine brief pain inventory assessments, showing no significant differences between treatment groups.Conclusion: Post-operative rehabilitation for MPL correction may enhance limb usage, joint function, muscle mass, and pain relief. However, the duration and level of post-operative pain may influence the necessity for rehabilitation. In addition, ES and LASER therapy offer similar pain-relieving effects after MPL surgery; therefore, the choice between these methods depends on the availability of equipment and veterinarian preferences.Keywords: electrical stimulation, light amplification by stimulated emission of radiation therapy, medial patellar luxation, post-operative management, rehabilitation.
背景和目的:电刺激(ES)和受激辐射光放大(LASER)疗法经常用于术后康复;但是,目前对这两种疗法的有效性进行比较的研究尚不充分。本研究旨在通过比较ES和LASER疗法与运动相结合的效果,评估髌骨内侧外翻(MPL)手术矫正后的术后康复效果。材料和方法:我们对接受过 MPL 手术治疗的狗进行了前瞻性临床试验。这些狗被分为以下三组:对照组:不参加任何术后康复项目;ES 组:接受 ES 治疗的术后康复项目;LASER 组:接受以 LASER 治疗为特色的术后康复项目:结果:各组在跛行评分、疼痛评分、大腿肌肉周长和活动范围等评估参数方面无明显差异。虽然某些组的疼痛评分可能存在差异,但这可能与患者术前的状况有关。这些结果与犬主问卷的犬类简短疼痛清单评估结果一致,显示治疗组之间没有显著差异:结论:MPL 矫正术后康复可提高肢体使用率、关节功能、肌肉质量和疼痛缓解率。然而,术后疼痛的持续时间和程度可能会影响康复治疗的必要性。此外,ES疗法和激光疗法在MPL术后具有相似的止痛效果;因此,在这两种方法之间做出选择取决于设备的可用性和兽医的偏好。关键词:电刺激;刺激辐射光放大疗法;髌骨内侧松弛症;术后管理;康复。
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引用次数: 0
Anatomical, pathological, and histological features of experimental respiratory infection of birds by biofilm-forming bacteria Staphylococcus aureus 生物膜形成菌金黄色葡萄球菌对鸟类呼吸道感染实验的解剖学、病理学和组织学特征
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.612-619
Ekaterina Lenchenko, N. Sachivkina, Olesya Petrukhina, N. Petukhov, Andrey Zharov, N. Zhabo, M. Avdonina
Background and Aim: The pathogenesis of staphylococcal infections is mediated by virulence factors, such as enzymes, toxins, and biofilms, which increase the resistance of microorganisms to host immune system evasion. Testing and searching for standardized multi-level algorithms for the indication and differentiation of biofilms at the early stages of diagnosis will contribute to the development of preventive measures to control the critical points of technology and manage dangerous risk factors for the spread of infectious diseases. This research aimed to study the main stages of Staphylococcus aureus biofilm formation in in vitro experiments and to analyze the dynamics of respiratory syndrome development in chickens infected with these bacteria.Materials and Methods: Experimental reproduction of the infectious process was performed using laboratory models: 10-day-old White Leghorn chickens (n = 20). Before the experiments, the birds were divided into two groups according to the principle of analogs: Group I (control, n = 10): the birds were intranasally inoculated with 0.5 cm3 of 0.9% NaCl solution; Group II (experiment, n = 10): the birds were intranasally inoculated with a suspension of S. aureus bacteria, 0.5 cm3, concentration 1 billion/cm3.Results: Colonization of individual areas of the substrate under study in vitro occurred gradually from the sedimentation and adhesion of single motile planktonic cells to the attachment stage of microcolony development. Staining preparations with gentian violet due to the “metachromosia” property of this dye are a quick and fairly simple way to differentiate cells and the intercellular matrix of biofilms. Fixation with vapors of glutaraldehyde and osmium tetroxide preserves the natural architecture of biofilms under optical and scanning electron microscopy. Pure cultures of S. aureus microorganisms were isolated from the blood, lungs, small intestine, liver, kidneys, and spleen after 5–10 days during experimental infection of chickens. Clinical signs of respiratory syndrome developed within 5–6 days after infection. Acute and subacute serousfibrinous airsacculitis, characterized by edema and thickening of the membranes of the air sacs and the presence of turbid, watery, foamy contents in the cavity, was the most characteristic pathomorphological sign. The signs of acute congestive hyperemia and one-sided serous-fibrinous pneumonia developed with significant thickening of fibrinous deposits. In Garder’s gland, there was an increase in the number of secretory sections, indicating hypersecretion of the glands. In the lymphoid follicles of Meckel’s diverticulum, leukocytes, usually lymphocytes, and pseudoeosinophils were detected.Conclusion: Hydration and heteromorphism of the internal environment of biofilms determine the localization of differentiated cells in a three-dimensional matrix for protection against adverse factors. The most characteristic pathomorphological sign was the develo
背景和目的:葡萄球菌感染的致病机理是由毒力因子介导的,如酶、毒素和生物膜,它们增加了微生物对宿主免疫系统逃避的抵抗力。在诊断的早期阶段测试和寻找用于指示和区分生物膜的标准化多级算法,将有助于制定预防措施,控制技术的关键点,管理传染病传播的危险风险因素。本研究旨在研究体外实验中金黄色葡萄球菌生物膜形成的主要阶段,并分析感染这些细菌的鸡呼吸道综合征的发展动态:使用实验室模型对感染过程进行实验重现:10 日龄的白羽肉鸡(n = 20)。实验前,根据类比原则将鸡分为两组:第一组(对照组,n = 10):给鸡鼻内接种 0.5 cm3 的 0.9% 氯化钠溶液;第二组(实验组,n = 10):给鸡鼻内接种 0.5 cm3 的金黄色葡萄球菌悬浮液,浓度为 10 亿/cm3:结果:体外研究中基质上各个区域的定殖是从单个运动浮游细胞的沉积和粘附逐渐发展到微菌落发育的附着阶段。由于龙胆紫具有 "变色 "特性,因此用龙胆紫染色制备生物膜是区分细胞和生物膜细胞间基质的一种快速而简单的方法。在光学显微镜和扫描电子显微镜下,用戊二醛和四氧化锇的蒸气进行固定可保留生物膜的天然结构。在鸡实验性感染 5--10 天后,从血液、肺部、小肠、肝脏、肾脏和脾脏中分离出纯净的金黄色葡萄球菌微生物培养物。感染后 5--6 天内出现呼吸道综合征的临床症状。急性和亚急性浆液性纤维素性气囊炎的特征是气囊膜水肿、增厚,气囊腔内有浑浊、水样、泡沫状内容物,这是最典型的病理形态学特征。急性充血性充血和一侧浆液性纤维蛋白性肺炎的体征随着纤维蛋白沉积物的明显增厚而出现。在加德腺,分泌物切片数量增加,表明腺体分泌过多。在梅克尔憩室的淋巴滤泡中,检测到白细胞(通常是淋巴细胞)和假嗜酸粒细胞:生物膜内部环境的水合性和异形性决定了分化细胞在三维基质中的定位,以抵御不利因素的影响。在易感模型中再现感染过程时,最典型的病理形态学特征是急性和亚急性浆液性纤维素性气囊炎的发展。纤维素沉积明显增厚,出现急性充血高血压症状,并出现一到两次浆液性-纤维素性肺炎。关键词:气囊炎;禽;生物膜;发病机制;呼吸综合征肺炎;金黄色葡萄球菌。
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引用次数: 0
Spatial and temporal distribution of foot-and-mouth disease outbreaks in Algeria from 2014 to 2022 2014 至 2022 年阿尔及利亚口蹄疫疫情的时空分布情况
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.509-517
Meryem Guessoum, M. Adnane, R. Baazizi, Madina-Saliha Derguini
Background and Aim: Foot-and-mouth disease (FMD), a major transboundary animal ailment in Algeria, is a serious economic burden on the livestock sector. This study aimed to investigate the spatiotemporal distribution of FMD in Algeria and identifies the factors contributing to this phenomenon.Materials and Methods: Data on FMD cases occurring in Algeria from 2014 to 2022 were collected from various sources, including archives at the Ministry of Agriculture, peer-reviewed journal articles, conference proceedings, reference laboratory reports, and unpublished scientific reports. The data were compiled and analyzed using MS Office Excel® and SPSS® software.Results: A total of 22,690 FMD cases and 1,141 outbreaks were reported in Algeria between 2014 and 2022. The apex of infections occurred in 2014 (34.5%), followed by an increase in the number of infections in 2019 and 2017. The prevalence of FMD extended to 91.6% of the districts of the country, particularly in the north (center) and eastern regions. Cows were the most affected, with 654 outbreaks and more than 3,665 cases. Although FMD affected all four regions, there was a statistically significant long-term decline in the incidence.Conclusion: These spatial and temporal trends underscore the robust disease control methodologies implemented by the Algerian government, such as the strategic immunization of livestock to fortify their viral resistance, stringent constraints on animal mobility, and enlightenment of farmers regarding the hazards associated with unrestricted livestock movement to effectively curb FMD dissemination.Keywords: control measures, distribution, foot-and-mouth disease, livestock, outbreaks.
背景和目的:口蹄疫(FMD)是阿尔及利亚的一种主要跨境动物疾病,对畜牧业造成了严重的经济负担。本研究旨在调查口蹄疫在阿尔及利亚的时空分布情况,并确定导致这一现象的因素:从各种来源收集了 2014 年至 2022 年阿尔及利亚发生的口蹄疫病例数据,包括农业部档案、同行评审期刊论文、会议记录、参考实验室报告和未发表的科学报告。数据使用 MS Office Excel® 和 SPSS® 软件进行汇编和分析:结果:2014 年至 2022 年期间,阿尔及利亚共报告了 22,690 例口蹄疫病例和 1,141 次疫情爆发。感染高峰出现在 2014 年(34.5%),随后在 2019 年和 2017 年感染数量有所增加。口蹄疫的流行范围扩大到全国 91.6% 的地区,尤其是北部(中部)和东部地区。奶牛受影响最大,共发生 654 起疫情,超过 3 665 例病例。尽管口蹄疫影响了所有四个地区,但从统计学角度看,发病率长期显著下降:这些空间和时间趋势凸显了阿尔及利亚政府实施的强有力的疾病控制方法,如对牲畜进行战略性免疫以增强其对病毒的抵抗力、严格限制牲畜的流动性以及向农民宣传牲畜无限制流动的危害,从而有效遏制口蹄疫的传播。
{"title":"Spatial and temporal distribution of foot-and-mouth disease outbreaks in Algeria from 2014 to 2022","authors":"Meryem Guessoum, M. Adnane, R. Baazizi, Madina-Saliha Derguini","doi":"10.14202/vetworld.2024.509-517","DOIUrl":"https://doi.org/10.14202/vetworld.2024.509-517","url":null,"abstract":"Background and Aim: Foot-and-mouth disease (FMD), a major transboundary animal ailment in Algeria, is a serious economic burden on the livestock sector. This study aimed to investigate the spatiotemporal distribution of FMD in Algeria and identifies the factors contributing to this phenomenon.\u0000\u0000Materials and Methods: Data on FMD cases occurring in Algeria from 2014 to 2022 were collected from various sources, including archives at the Ministry of Agriculture, peer-reviewed journal articles, conference proceedings, reference laboratory reports, and unpublished scientific reports. The data were compiled and analyzed using MS Office Excel® and SPSS® software.\u0000\u0000Results: A total of 22,690 FMD cases and 1,141 outbreaks were reported in Algeria between 2014 and 2022. The apex of infections occurred in 2014 (34.5%), followed by an increase in the number of infections in 2019 and 2017. The prevalence of FMD extended to 91.6% of the districts of the country, particularly in the north (center) and eastern regions. Cows were the most affected, with 654 outbreaks and more than 3,665 cases. Although FMD affected all four regions, there was a statistically significant long-term decline in the incidence.\u0000\u0000Conclusion: These spatial and temporal trends underscore the robust disease control methodologies implemented by the Algerian government, such as the strategic immunization of livestock to fortify their viral resistance, stringent constraints on animal mobility, and enlightenment of farmers regarding the hazards associated with unrestricted livestock movement to effectively curb FMD dissemination.\u0000\u0000Keywords: control measures, distribution, foot-and-mouth disease, livestock, outbreaks.","PeriodicalId":506834,"journal":{"name":"Veterinary World","volume":"48 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140085634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans 小鼠、大鼠和人类精子和雄性生殖道中 Siglec-1 蛋白网络和表达模式的比较
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.645-657
H. Almhanna, A. Kumar, David Kilroy, Gina Duggan, Jane A. Irwin, Bridget Hogg, Colm Reid
Background and Aim: Sialic acid-binding immunoglobulin-like lectin 1 (Siglec-1) is a transmembrane glycoprotein involved in the sialic acid (Sia)-dependent regulation of the immune system. Siglec-1 expression has recently been identified in the male reproductive tract (MRT) of several species, including humans, cattle, horses, and sheep, and may play a role in modulating fertility in a Sia-dependent manner.Materials and Methods: In this study, protein–protein interaction (PPI) analysis of Siglec-1 was conducted to identify associated network protein conservation, and the expression of Siglec-1 in the MRT of mice and rats, including their accessory sex glands and spermatozoa was determined by immunostaining.Results: Network analysis of proteins with Siglec-1 in mice and rats demonstrated significant similarity to human Siglec-1 networks, suggesting a similar conservation of network proteins between these species and, hence, a potential conservation role in immune modulation and function. Specific immunostaining patterns of mouse and rat testes, epididymis, ductus deferens, accessory sex gland tissues, and sperm were detected using human Siglec-1. These results confirmed that the human Siglec-1 antibody could cross-react with mouse and rat Siglec-1, suggesting that the specific expression patterns of Siglec-1 in the MRT and sperm of both mice and rats are similar to those observed in other species.Conclusion: The conservation of Siglec-1 expression patterns in sperm and within the MRT and the similarity of protein networks for Siglec-1 across species suggest that Siglec-1 may function in a similar manner across species. These results also suggest that rodents may serve as a valuable model system for exploring the function of Siglecs in the reproductive system across species and their potential role in modulating fertility in a Sia-dependent manner.Keywords: female reproductive tract, male reproductive tract, protein–protein interaction, sialic acid, sialic acid-binding immunoglobulin-like lectin-1.
背景与目的:硅铝酸结合免疫球蛋白样凝集素 1(Siglec-1)是一种跨膜糖蛋白,参与硅铝酸(Sia)对免疫系统的依赖性调控。最近在包括人、牛、马和羊在内的多个物种的雄性生殖道(MRT)中发现了 Siglec-1 的表达,它可能以依赖 Sia 的方式在调节生育能力方面发挥作用:本研究对Siglec-1进行了蛋白质相互作用(PPI)分析,以确定相关的网络蛋白质保护,并通过免疫染色法测定了Siglec-1在小鼠和大鼠MRT(包括附属性腺和精子)中的表达:结果:对小鼠和大鼠体内带有Siglec-1的蛋白质进行网络分析后发现,这些蛋白质与人类的Siglec-1网络非常相似,这表明这些物种之间存在类似的网络蛋白质保护,因此在免疫调节和功能方面存在潜在的保护作用。使用人类 Siglec-1 检测了小鼠和大鼠睾丸、附睾、输精管、附属性腺组织和精子的特异性免疫染色模式。这些结果证实人 Siglec-1 抗体可与小鼠和大鼠 Siglec-1 发生交叉反应,表明 Siglec-1 在小鼠和大鼠 MRT 和精子中的特异性表达模式与在其他物种中观察到的模式相似:结论:Siglec-1在精子和MRT中的表达模式以及Siglec-1的蛋白质网络在不同物种间的相似性表明,Siglec-1可能以类似的方式在不同物种间发挥作用。关键词:雌性生殖道 雄性生殖道 蛋白相互作用 Sialic acid Sialic acid结合免疫球蛋白样凝集素-1。
{"title":"Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans","authors":"H. Almhanna, A. Kumar, David Kilroy, Gina Duggan, Jane A. Irwin, Bridget Hogg, Colm Reid","doi":"10.14202/vetworld.2024.645-657","DOIUrl":"https://doi.org/10.14202/vetworld.2024.645-657","url":null,"abstract":"Background and Aim: Sialic acid-binding immunoglobulin-like lectin 1 (Siglec-1) is a transmembrane glycoprotein involved in the sialic acid (Sia)-dependent regulation of the immune system. Siglec-1 expression has recently been identified in the male reproductive tract (MRT) of several species, including humans, cattle, horses, and sheep, and may play a role in modulating fertility in a Sia-dependent manner.\u0000\u0000Materials and Methods: In this study, protein–protein interaction (PPI) analysis of Siglec-1 was conducted to identify associated network protein conservation, and the expression of Siglec-1 in the MRT of mice and rats, including their accessory sex glands and spermatozoa was determined by immunostaining.\u0000\u0000Results: Network analysis of proteins with Siglec-1 in mice and rats demonstrated significant similarity to human Siglec-1 networks, suggesting a similar conservation of network proteins between these species and, hence, a potential conservation role in immune modulation and function. Specific immunostaining patterns of mouse and rat testes, epididymis, ductus deferens, accessory sex gland tissues, and sperm were detected using human Siglec-1. These results confirmed that the human Siglec-1 antibody could cross-react with mouse and rat Siglec-1, suggesting that the specific expression patterns of Siglec-1 in the MRT and sperm of both mice and rats are similar to those observed in other species.\u0000\u0000Conclusion: The conservation of Siglec-1 expression patterns in sperm and within the MRT and the similarity of protein networks for Siglec-1 across species suggest that Siglec-1 may function in a similar manner across species. These results also suggest that rodents may serve as a valuable model system for exploring the function of Siglecs in the reproductive system across species and their potential role in modulating fertility in a Sia-dependent manner.\u0000\u0000Keywords: female reproductive tract, male reproductive tract, protein–protein interaction, sialic acid, sialic acid-binding immunoglobulin-like lectin-1.","PeriodicalId":506834,"journal":{"name":"Veterinary World","volume":"33 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140279866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of expanding farmlands with domestication of animals in the vicinity of disturbed swamps and built-up farmland ponds on population dispersion and decline of locally adapted Mansonia vectors (Diptera: Culicidae) 在受干扰的沼泽和已建成的农田池塘附近扩大农田并驯化动物对适应当地环境的曼氏病媒(双翅目:蚤科)的种群扩散和衰退的影响
Pub Date : 2024-03-01 DOI: 10.14202/vetworld.2024.564-576
Suntorn Pimnon, A. Bhumiratana, A. Intarapuk, W. Ritthison
Background and Objectives: The adaptive processes and resilience of Mansonia vectors responsible for bioindicators can change in response to climate, land use, and environmental changes. This study evaluated the effects of expanding farmlands with the domestication of animals in the vicinity of either disturbed swamps or built-up farmland ponds on the population dispersion and decline of locally adapted Mansonia faunas as a result of expanding farmlands in Thailand.Materials and Methods: Based on environmental surveys, four different geographically defined study sites were selected: I - the expanding farmlands with domestication of livestock and pet animals in the vicinity of low-lying swamp with habitat fragmentation and aquatic vegetation; II - the expanding farmlands with domestication of pet animals in the vicinity of elevated swamp with habitat destruction and aquatic vegetation; III - the expanding farmlands with domestication of livestock and pet animals in the vicinity of low-lying farmland ponds with restoration and aquatic vegetation; and IV - the expanding farmlands with domestication of pet animals in the vicinity of elevated farmland ponds with restoration and aquatic vegetation. Human landing catch collection method was used to periodically assess the species composition and abundance of Mansonia vectors.Results: Aggregated distributions and seasonal abundances of Mansonia faunas (Mansonia uniformis, Mansonia indiana, Mansonia annulifera, Mansonia annulata, Mansonia bonneae, and Mansonia dives) with variable proportions were observed at all the study sites. A decline in the population of Mansonia faunas, except for Ma. uniformis, was observed at study sites I and II.Conclusion: The anticipated effects of expanding farmlands affected the population dispersion and decline of locally adapted Mansonia faunas, thus representing a diverse assemblage of Mansonia species with different adaptations, ecological tolerances, and host exploitation strategies in life. These effects depended either on the function of disturbed swamps or on the development of farmland ponds, whether they provided a wide range of freshwater habitats, or on the domestication of animals, whether they provided animal blood meal sources.Keywords: built-up farmland ponds, disturbed swamps, expanding farmland, freshwater habitats, and animal blood meal sources, Mansonia fauna.
背景与目标:负责生物指标的 Mansonia 向量的适应过程和复原力会随着气候、土地利用和环境变化而改变。本研究评估了在泰国受干扰的沼泽或已建成的农田池塘附近扩大农田和驯化动物对适应当地环境的 Mansonia 动物群的种群分散和减少的影响:根据环境调查,选择了四个不同的地理研究地点:I - 在低洼沼泽附近驯养家畜和宠物动物的农田不断扩大,生境破碎,水生植被破坏;II - 在高地沼泽附近驯养宠物动物的农田不断扩大,生境破坏,水生植被破坏;III - 在低洼农田池塘附近驯养家畜和宠物动物的农田不断扩大,生境恢复,水生植被破坏;IV - 在高地农田池塘附近驯养宠物动物的农田不断扩大,生境恢复,水生植被破坏。采用人工登陆捕捉法,定期评估曼氏藻类病媒的物种组成和丰度:结果:在所有研究地点都观察到了不同比例的 Mansonia 动物群(Mansonia uniformis、Mansonia indiana、Mansonia annulifera、Mansonia annulata、Mansonia bonneae 和 Mansonia dives)的聚集分布和季节丰度。在研究地点 I 和 II 观察到 Mansonia 动物群数量减少,但 Ma. uniformis 除外:结论:农田扩大的预期效应影响了适应当地环境的 Mansonia 动物群的种群分散和数量下降,从而代表了具有不同适应性、生态耐受性和寄主开发策略的 Mansonia 物种的多样性组合。这些影响或者取决于受干扰沼泽的功能,或者取决于农田池塘的发展,它们是否提供了广泛的淡水生境,或者取决于动物的驯化,它们是否提供了动物血粉来源。关键词:建成的农田池塘;受干扰的沼泽;扩大的农田;淡水生境;动物血粉来源;Mansonia动物群。
{"title":"Effect of expanding farmlands with domestication of animals in the vicinity of disturbed swamps and built-up farmland ponds on population dispersion and decline of locally adapted Mansonia vectors (Diptera: Culicidae)","authors":"Suntorn Pimnon, A. Bhumiratana, A. Intarapuk, W. Ritthison","doi":"10.14202/vetworld.2024.564-576","DOIUrl":"https://doi.org/10.14202/vetworld.2024.564-576","url":null,"abstract":"Background and Objectives: The adaptive processes and resilience of Mansonia vectors responsible for bioindicators can change in response to climate, land use, and environmental changes. This study evaluated the effects of expanding farmlands with the domestication of animals in the vicinity of either disturbed swamps or built-up farmland ponds on the population dispersion and decline of locally adapted Mansonia faunas as a result of expanding farmlands in Thailand.\u0000\u0000Materials and Methods: Based on environmental surveys, four different geographically defined study sites were selected: I - the expanding farmlands with domestication of livestock and pet animals in the vicinity of low-lying swamp with habitat fragmentation and aquatic vegetation; II - the expanding farmlands with domestication of pet animals in the vicinity of elevated swamp with habitat destruction and aquatic vegetation; III - the expanding farmlands with domestication of livestock and pet animals in the vicinity of low-lying farmland ponds with restoration and aquatic vegetation; and IV - the expanding farmlands with domestication of pet animals in the vicinity of elevated farmland ponds with restoration and aquatic vegetation. Human landing catch collection method was used to periodically assess the species composition and abundance of Mansonia vectors.\u0000\u0000Results: Aggregated distributions and seasonal abundances of Mansonia faunas (Mansonia uniformis, Mansonia indiana, Mansonia annulifera, Mansonia annulata, Mansonia bonneae, and Mansonia dives) with variable proportions were observed at all the study sites. A decline in the population of Mansonia faunas, except for Ma. uniformis, was observed at study sites I and II.\u0000\u0000Conclusion: The anticipated effects of expanding farmlands affected the population dispersion and decline of locally adapted Mansonia faunas, thus representing a diverse assemblage of Mansonia species with different adaptations, ecological tolerances, and host exploitation strategies in life. These effects depended either on the function of disturbed swamps or on the development of farmland ponds, whether they provided a wide range of freshwater habitats, or on the domestication of animals, whether they provided animal blood meal sources.\u0000\u0000Keywords: built-up farmland ponds, disturbed swamps, expanding farmland, freshwater habitats, and animal blood meal sources, Mansonia fauna.","PeriodicalId":506834,"journal":{"name":"Veterinary World","volume":"1 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140270261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Tonovet® and Tonovet plus® tonometers for measuring intraocular pressure in dogs, cats, horses, cattle, and sheep 用于测量狗、猫、马、牛和羊眼内压的 Tonovet® 和 Tonovet plus® 眼压计的比较
Pub Date : 2024-02-01 DOI: 10.14202/vetworld.2024.384-388
L. Kovaļčuka, A. Mālniece, Jana Vanaga
Background and Aim: Reference ranges for intraocular pressure (IOP) in healthy animals are device-specific; therefore, it is strongly recommended to use appropriate reference values according to the device. Therefore, our aim was to compare IOP readings made by TonoVet® and TonoVet Plus® in healthy dogs, cats, sheep, cattle, and horses. We compared IOP values measured by TonoVet® and TonoVet Plus® tonometers in clinically normal eyes of dogs, cats, horses, cattle, and sheep.Materials and Methods: Five groups comprising 20 animals each of dogs (various breeds, 9 months–10 years old, 14 females, 6 males), cats (various breeds, 6 months–12 years old, 8 females, 12 males), horses (various breeds, 5–12 years old, 12 females, 8 males), cattle (Holstein, 1–7 lactation, female), and sheep (Latvian Darkhead ewes, 1–8 years old) were included in the study. Both eyes of all animals were subjected to ophthalmic examination, including evaluation of IOP by rebound tonometry using TonoVet® and TonoVet Plus® devices. Normality was determined using the Shapiro–Wilk test. The independent t-test was used to determine differences between IOP values in the right and left eyes and between both tonometers. This study was approved by the Ethical Commission of the Latvia University of Life Sciences and Technologies (Nr. LLU_Dzaep_2022-2-4).Results: No differences in IOP between the right and left eyes were found in all cases (p > 0.05). The mean IOP ± standard deviation values in both eyes for TonoVet® and TonoVet Plus® tonometers were as follows: for dogs, 15.25 ± 2.73 mmHg and 19.65 ± 3.46 mmHg; and in cats, 18.88 ± 3.98 mmHg and 18.78 ± 4.26 mmHg, respectively. In horses, mean IOP was 22.15 ± 3.74 mmHg and 24.28 ± 3.00 mmHg; in cattle, 24.73 ± 2.89 mmHg and 23.28 ± 2.97 mmHg; and in sheep, 18.05 ± 3.54 mmHg and 22.49 ± 4.66 mmHg, respectively. Significant differences in IOP values were observed between the tonometers in sheep, dog, and horse groups (mean difference –4.40, –4.48, and 2.13, respectively).Conclusion: This study showed significantly higher IOP values measured by the TonoVet Plus® tonometer in dogs and sheep.Keywords: cats, cattle, dogs, horses, sheep, TonoVet plus, TonoVet.
背景和目的:健康动物眼内压 (IOP) 的参考范围因设备而异;因此,强烈建议根据设备使用适当的参考值。因此,我们的目的是比较 TonoVet® 和 TonoVet Plus® 对健康狗、猫、羊、牛和马的眼压读数。我们比较了 TonoVet® 和 TonoVet Plus® 眼压计在临床上正常的狗、猫、马、牛和羊眼中测量的眼压值:本研究共分为五组,每组 20 只,包括狗(不同品种,9 个月至 10 岁,14 只雌性,6 只雄性)、猫(不同品种,6 个月至 12 岁,8 只雌性,12 只雄性)、马(不同品种,5 至 12 岁,12 只雌性,8 只雄性)、牛(荷斯坦牛,1 至 7 个月,雌性)和绵羊(拉脱维亚黑头母羊,1 至 8 岁)。所有动物的双眼都接受了眼科检查,包括使用 TonoVet® 和 TonoVet Plus® 设备通过回弹式眼压计评估眼压。采用 Shapiro-Wilk 检验确定正态性。采用独立 t 检验确定左右眼眼压值之间以及两种眼压计之间的差异。本研究获得了拉脱维亚生命科学与技术大学伦理委员会的批准(编号:LLU_Dzaep_2022-2-4):所有病例的左右眼眼压均无差异(P > 0.05)。TonoVet® 和 TonoVet Plus® 眼压计的双眼平均眼压 ± 标准偏差值如下:狗分别为 15.25 ± 2.73 mmHg 和 19.65 ± 3.46 mmHg;猫分别为 18.88 ± 3.98 mmHg 和 18.78 ± 4.26 mmHg。马的平均眼压分别为 22.15 ± 3.74 mmHg 和 24.28 ± 3.00 mmHg;牛的平均眼压分别为 24.73 ± 2.89 mmHg 和 23.28 ± 2.97 mmHg;绵羊的平均眼压分别为 18.05 ± 3.54 mmHg 和 22.49 ± 4.66 mmHg。在羊、狗和马组中,眼压计之间的眼压值存在显著差异(平均差异分别为-4.40、-4.48和2.13):本研究表明,用 TonoVet Plus® 眼压计测量的狗和羊的眼压值明显更高。
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Veterinary World
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