Pub Date : 2024-03-15DOI: 10.29326/2304-196x-2024-13-1-44-56
N. N. Lugovskaya, E. A. Silanteva, T. V. Okovytaya, A. A. Kharitonova, Y. M. Gochmuradov, E. A. Razgulyaeva, O. O. Budina, E. A. Yasneva
A total of 138 serum samples from pigs and cattle vaccinated against foot-and-mouth disease virus (FMDV) of one or two serotypes or infected with FMDV were used for testing of 24 enzyme-linked immunosorbent assay (ELISA) diagnostic tests-systems for detection of antibodies against FMDV structural proteins produced by 6 manufacturers (Federal Centre for Animal Health, Prionics, IZSLER, Innovative Diagnostics, BIONOTE and MEDIAN Diagnostics) for their serotype-specificity. All used test-systems detected apparent serotype-specific activity (homologous reaction) as well as cross-reacting virus-specific antibodies that was accounted for some reasons related to conservative epitopes in amino acid sequence of FMDV virion capsid VP1–VP3 polypeptides, accessibility of internal conservative epitopes of VP4 polypeptide for the animal’s immune system during virus replication or vaccine antigen (virus) destruction in the animal’s body in the process of immunity development, as well as the pilot anti-FMD vaccine composition, etc. Nevertheless, the analysis of a large data set (about 3,500 tests) showed that the homologous serotype-specific reaction in general was significantly higher and predominant, the proportion of virus-specific non-protective antibodies, including cross-reacting ones, was not significant and did not distort the results of ELISA tests of anti-FMD vaccine for its immunogenicity. Inconclusive test results require confirmation with other serological tests. Complex tests for FMDV using different diagnostic methods such as ELISA with standard and reference test-systems and/or virus neutralization test in cell culture are to be considered as the best option.
{"title":"Testing of diagnostic test-systems for detection of antibodies to foot-and-mouth disease virus structural proteins with enzyme-linked immunosorbent assay for their serotype specificity","authors":"N. N. Lugovskaya, E. A. Silanteva, T. V. Okovytaya, A. A. Kharitonova, Y. M. Gochmuradov, E. A. Razgulyaeva, O. O. Budina, E. A. Yasneva","doi":"10.29326/2304-196x-2024-13-1-44-56","DOIUrl":"https://doi.org/10.29326/2304-196x-2024-13-1-44-56","url":null,"abstract":"A total of 138 serum samples from pigs and cattle vaccinated against foot-and-mouth disease virus (FMDV) of one or two serotypes or infected with FMDV were used for testing of 24 enzyme-linked immunosorbent assay (ELISA) diagnostic tests-systems for detection of antibodies against FMDV structural proteins produced by 6 manufacturers (Federal Centre for Animal Health, Prionics, IZSLER, Innovative Diagnostics, BIONOTE and MEDIAN Diagnostics) for their serotype-specificity. All used test-systems detected apparent serotype-specific activity (homologous reaction) as well as cross-reacting virus-specific antibodies that was accounted for some reasons related to conservative epitopes in amino acid sequence of FMDV virion capsid VP1–VP3 polypeptides, accessibility of internal conservative epitopes of VP4 polypeptide for the animal’s immune system during virus replication or vaccine antigen (virus) destruction in the animal’s body in the process of immunity development, as well as the pilot anti-FMD vaccine composition, etc. Nevertheless, the analysis of a large data set (about 3,500 tests) showed that the homologous serotype-specific reaction in general was significantly higher and predominant, the proportion of virus-specific non-protective antibodies, including cross-reacting ones, was not significant and did not distort the results of ELISA tests of anti-FMD vaccine for its immunogenicity. Inconclusive test results require confirmation with other serological tests. Complex tests for FMDV using different diagnostic methods such as ELISA with standard and reference test-systems and/or virus neutralization test in cell culture are to be considered as the best option.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":" 52","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140391956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.29326/2304-196x-2024-13-1-73-77
M. Baratov, A. R. Mustafayev
Contamination of the environment with the infectious animal disease agents is still a pressing problem for the poultry farms. Mycobacterium avium can grow and replicate in organic wastes from the poultry farms for a long time thus contaminating vast adjacent areas and being the source of infection not only for wild and domestic animals but also for humans. The studies were aimed at the examination of the duration of Mycobacterium avium survival in the natural environment in two geographical regions of the Republic of Dagestan characterized by different soil and climate. Samples of Mycobacterium avium-contaminated feces and soil collected from pastures and farmyards (on the surface and from 5 cm depth) were tested. The experiments demonstrated that pathogenic for chickens mycobacteria survived for up to 30 days in the samples collected in the sub-mountainous areas in summertime, when the air temperature ranged from 15.1 to 30 °С, land surface temperature – from 17 to 38 °С, air humidity – from 44 to 94% and average monthly precipitation amounted to 1.5 mm. From September to May, with the air temperature ranging from –10.8 to +25 °C, land surface temperature from –14 to +30 °C, air humidity 26–100% and average precipitation 0.39 mm, the bacteria survived for up to 213 days on the soil surface on the pastures and farmyards, and for up to 243 days at the depth of 5 cm and in the feces. In the plain area, in the same time period in the slightly saline soil with high humus content and at air temperature from –11.9 to +27.3 °C, soil temperature from –13 to +45 °C, air humidity from 37 to 100% and average precipitation 20.4 mm, Mycobacterium avium survived just like in the sub-mountainous area, i.e. for 213 and 243 days, respectively. The post-mortem lesions in the internal organs of the poultry corresponded to the tuberculosis clinical signs in 86 of 171 birds (50.3%). The study results will allow for the development of the optimal algorithm for animal health and management measures aimed at tuberculosis eradication on the poultry farms.
{"title":"Scientific justification of Mycobacterium avium survival in natural environment of Republic of Dagestan","authors":"M. Baratov, A. R. Mustafayev","doi":"10.29326/2304-196x-2024-13-1-73-77","DOIUrl":"https://doi.org/10.29326/2304-196x-2024-13-1-73-77","url":null,"abstract":"Contamination of the environment with the infectious animal disease agents is still a pressing problem for the poultry farms. Mycobacterium avium can grow and replicate in organic wastes from the poultry farms for a long time thus contaminating vast adjacent areas and being the source of infection not only for wild and domestic animals but also for humans. The studies were aimed at the examination of the duration of Mycobacterium avium survival in the natural environment in two geographical regions of the Republic of Dagestan characterized by different soil and climate. Samples of Mycobacterium avium-contaminated feces and soil collected from pastures and farmyards (on the surface and from 5 cm depth) were tested. The experiments demonstrated that pathogenic for chickens mycobacteria survived for up to 30 days in the samples collected in the sub-mountainous areas in summertime, when the air temperature ranged from 15.1 to 30 °С, land surface temperature – from 17 to 38 °С, air humidity – from 44 to 94% and average monthly precipitation amounted to 1.5 mm. From September to May, with the air temperature ranging from –10.8 to +25 °C, land surface temperature from –14 to +30 °C, air humidity 26–100% and average precipitation 0.39 mm, the bacteria survived for up to 213 days on the soil surface on the pastures and farmyards, and for up to 243 days at the depth of 5 cm and in the feces. In the plain area, in the same time period in the slightly saline soil with high humus content and at air temperature from –11.9 to +27.3 °C, soil temperature from –13 to +45 °C, air humidity from 37 to 100% and average precipitation 20.4 mm, Mycobacterium avium survived just like in the sub-mountainous area, i.e. for 213 and 243 days, respectively. The post-mortem lesions in the internal organs of the poultry corresponded to the tuberculosis clinical signs in 86 of 171 birds (50.3%). The study results will allow for the development of the optimal algorithm for animal health and management measures aimed at tuberculosis eradication on the poultry farms.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140391329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.29326/2304-196x-2023-12-4-345-353
E. A. Trofimova, S. V. Kononova, I. Shumilova, B. L. Manin
Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin.
{"title":"Goat-derived cell line (Capra hircus) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum","authors":"E. A. Trofimova, S. V. Kononova, I. Shumilova, B. L. Manin","doi":"10.29326/2304-196x-2023-12-4-345-353","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-4-345-353","url":null,"abstract":"Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"17 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.29326/2304-196x-2023-12-4-322-330
L. I. Drozdova, A. S. Barkova, M. N. Isakova, L. P. Larionov, V. V. Permikin, N. M. Starikov, T. G. Khonina
Cow’s milk quality, which may deteriorate due to inflammatory processes in the mammary glands, remains one of the important problems of dairy farming and requires effective, safe and affordable therapeutic agents. Nanocomposite silicon-zinc-boron-containing glycerohydrogel (Si-Zn-B-gel) may become a very good solution to the issue. The paper demonstrates wound-healing effect of the glycerohydrogel and confirms its effectiveness for teat hyperkeratosis treatment indairy cows. Results of a rat burn model – based experiment suggest that Si-Zn-B-gel is a promising wound healing agent for topical use. Thus, on Day 9 complete re-epithelialization of the burn surface was observed, with fibrous structures prevailing in the granulation tissue of the dermal layer, on Day 19 a mature scar was formed with a longitudinal alignment of collagen fibers. The production tests conducted inhigh producing dairy cows have demonstrated good therapeutic effect of the Si-Zn-B gel for teat-end hyperkeratosis and confirmed its long-term effect that helps to longer maintain the results achieved during treatment. After a 7-day treatment physiological structure of up to 27.8% teats improved, on Day 14 of the experiment no severe hyperkeratotic lesions were observed and the number of teats that correspond to the physiological norm was 72.2%. Analysis of the data collected shows that the Si-Zn-B-gel is effective for teat-end hyperkeratosis treatment, thus, it prevents mastitis in animals and improves the milk quality.
{"title":"Evaluating wound-healing effect of silicon-zinc-boron-containing glycerohydrogel and its effect on mammary glands of high producing dairy cows","authors":"L. I. Drozdova, A. S. Barkova, M. N. Isakova, L. P. Larionov, V. V. Permikin, N. M. Starikov, T. G. Khonina","doi":"10.29326/2304-196x-2023-12-4-322-330","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-4-322-330","url":null,"abstract":"Cow’s milk quality, which may deteriorate due to inflammatory processes in the mammary glands, remains one of the important problems of dairy farming and requires effective, safe and affordable therapeutic agents. Nanocomposite silicon-zinc-boron-containing glycerohydrogel (Si-Zn-B-gel) may become a very good solution to the issue. The paper demonstrates wound-healing effect of the glycerohydrogel and confirms its effectiveness for teat hyperkeratosis treatment indairy cows. Results of a rat burn model – based experiment suggest that Si-Zn-B-gel is a promising wound healing agent for topical use. Thus, on Day 9 complete re-epithelialization of the burn surface was observed, with fibrous structures prevailing in the granulation tissue of the dermal layer, on Day 19 a mature scar was formed with a longitudinal alignment of collagen fibers. The production tests conducted inhigh producing dairy cows have demonstrated good therapeutic effect of the Si-Zn-B gel for teat-end hyperkeratosis and confirmed its long-term effect that helps to longer maintain the results achieved during treatment. After a 7-day treatment physiological structure of up to 27.8% teats improved, on Day 14 of the experiment no severe hyperkeratotic lesions were observed and the number of teats that correspond to the physiological norm was 72.2%. Analysis of the data collected shows that the Si-Zn-B-gel is effective for teat-end hyperkeratosis treatment, thus, it prevents mastitis in animals and improves the milk quality.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"25 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-240-245
M. D. Lozovoy, S. Shcherbinin, A. Karaulov
African swine fever is a highly contagious viral disease of pigs; however, no vaccines are available to control it. Currently the disease causes significant economic damage in many countries. The Republic of China is the first country in Southeast Asia, which officially reported the African swine fever outbreak in 2018. The disease further spread to all provinces of the country with 200 outbreaks in domestic pigs and 10 outbreaks in wild boar in total; herewith the overall population of pigs de- creased dramatically, approximately by 180 million animals. Following the confirmation of the first African swine fever outbreak, the PRC Ministry of Agriculture and Rural Affairs launched the “African Swine Fever Contingency Plan and Emergency Response”, which facilitated the disease eradication and already by 2023, no new infection outbreaks had been reported. The country with the largest swine population in the world achieved rather moderate spread rates if compared to Europe. In addition, the virus strains, which circulated in the Chinese territory, were genetically diverse and different in virulence, leading to a wide range of clinical signs manifested by diseased animals. Such aspects were supposed to complicate the eradication measures, but instead, they proved their effectiveness. The experience gained from the disease control in China is most certainly of interest for the Russian Federation, infected with African swine fever since 2008.
{"title":"Specific features of African swine fever control activities in China","authors":"M. D. Lozovoy, S. Shcherbinin, A. Karaulov","doi":"10.29326/2304-196x-2023-12-3-240-245","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-240-245","url":null,"abstract":"African swine fever is a highly contagious viral disease of pigs; however, no vaccines are available to control it. Currently the disease causes significant economic damage in many countries. The Republic of China is the first country in Southeast Asia, which officially reported the African swine fever outbreak in 2018. The disease further spread to all provinces of the country with 200 outbreaks in domestic pigs and 10 outbreaks in wild boar in total; herewith the overall population of pigs de- creased dramatically, approximately by 180 million animals. Following the confirmation of the first African swine fever outbreak, the PRC Ministry of Agriculture and Rural Affairs launched the “African Swine Fever Contingency Plan and Emergency Response”, which facilitated the disease eradication and already by 2023, no new infection outbreaks had been reported. The country with the largest swine population in the world achieved rather moderate spread rates if compared to Europe. In addition, the virus strains, which circulated in the Chinese territory, were genetically diverse and different in virulence, leading to a wide range of clinical signs manifested by diseased animals. Such aspects were supposed to complicate the eradication measures, but instead, they proved their effectiveness. The experience gained from the disease control in China is most certainly of interest for the Russian Federation, infected with African swine fever since 2008.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"192 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139344226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-215-221
S. V. Ivanova, L. A. Melnikova, A. P. Rodionov, V. Evstifeev
Anthrax is a highly dangerous disease of animals and humans caused by the spore-forming bacterium Bacillus anthracis. Currently, the disease is widespread in many countries of the world. Many regions of the Russian Federation are anthrax-endemic. A large number of anthrax treatment, diagnosis and prevention tools are developed using hyperimmune serum. Currently known commercial hyperimmune sera are produced by 2-month long immunization of horses, which is a long and expensive process. This suggests the need to develop faster and cheaper ways to produce anti-anthrax hyperimmune sera; such possible ways became the objective of this study. A live culture of Bacillus anthracis 55-VNIIVViM vaccine strain, used to produce live vaccines against animal anthrax, was used in the experiments. Rabbits were used as animal models. Based on the findings the method of rabbit immunization was selected. The optimal method included intravenous injection of the antigen in increasing amounts according to the following scheme: injection I – 0.5 cm 3; injection II – 1 cm3; injection III – 2 cm3 at a dose of100million mc / animal in 1cm 3, with 4-day interval between injections. This scheme made it possible to produce the serum with a high antibody titer equal to 14 log2. For long-term storage of the serum produced, the freeze-drying modes were optimized, giving 2% residual moisture content of the finished product. The analysis of the freeze-dried serum storage terms showed that the initial activity and physico-chemical properties of the product are maintained for 30 months.
炭疽病是由炭疽芽孢杆菌（Bacillus anthracis）引起的一种高度危险的人畜疾病。目前，这种疾病在世界许多国家广泛流行。俄罗斯联邦的许多地区都是炭疽病流行区。利用超免疫血清开发了大量炭疽治疗、诊断和预防工具。目前已知的商业超免疫血清是通过对马进行长达 2 个月的免疫来生产的，这是一个漫长而昂贵的过程。这表明有必要开发更快、更便宜的方法来生产抗炭疽超免疫血清；这种可能的方法成为本研究的目标。实验中使用了炭疽杆菌 55-VNIIVViM 疫苗菌株的活体培养物，该菌株用于生产动物炭疽活疫苗。兔子被用作动物模型。根据研究结果选择了兔免疫方法。最佳方法包括按照以下方案静脉注射抗原，注射量依次递增：注射 I - 0.5 cm 3；注射 II - 1 cm 3；注射 III - 2 cm 3，剂量为 1 亿微克/只（1 cm 3），每次注射间隔 4 天。通过这种方法可以生产出抗体滴度高达 14 log2 的血清。为了长期保存所生产的血清，对冷冻干燥模式进行了优化，使成品的残余水分含量达到 2%。对冻干血清储存条件的分析表明，产品的初始活性和理化特性可保持 30 个月。
{"title":"Method of obtaining and storing hyperimmune anthrax serum","authors":"S. V. Ivanova, L. A. Melnikova, A. P. Rodionov, V. Evstifeev","doi":"10.29326/2304-196x-2023-12-3-215-221","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-215-221","url":null,"abstract":"Anthrax is a highly dangerous disease of animals and humans caused by the spore-forming bacterium Bacillus anthracis. Currently, the disease is widespread in many countries of the world. Many regions of the Russian Federation are anthrax-endemic. A large number of anthrax treatment, diagnosis and prevention tools are developed using hyperimmune serum. Currently known commercial hyperimmune sera are produced by 2-month long immunization of horses, which is a long and expensive process. This suggests the need to develop faster and cheaper ways to produce anti-anthrax hyperimmune sera; such possible ways became the objective of this study. A live culture of Bacillus anthracis 55-VNIIVViM vaccine strain, used to produce live vaccines against animal anthrax, was used in the experiments. Rabbits were used as animal models. Based on the findings the method of rabbit immunization was selected. The optimal method included intravenous injection of the antigen in increasing amounts according to the following scheme: injection I – 0.5 cm 3; injection II – 1 cm3; injection III – 2 cm3 at a dose of100million mc / animal in 1cm 3, with 4-day interval between injections. This scheme made it possible to produce the serum with a high antibody titer equal to 14 log2. For long-term storage of the serum produced, the freeze-drying modes were optimized, giving 2% residual moisture content of the finished product. The analysis of the freeze-dried serum storage terms showed that the initial activity and physico-chemical properties of the product are maintained for 30 months.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-190-196
A. S. Yakovleva, А. V. Scherbakov
{"title":"Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review)","authors":"A. S. Yakovleva, А. V. Scherbakov","doi":"10.29326/2304-196x-2023-12-3-190-196","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-190-196","url":null,"abstract":"","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139345101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-222-227
V. Evstifeev, F. М. Khusainov, S. I. Yakovlev, R. I. Shangaraev, V. I. Eremets
Specific antibodies against сhlamydia were detected using complement fixation test and сhlamydia genome was detected using polymerase chain reaction in pregnant rabbits experimentally infected with Chlamydia psittaci. The infected rabbits developed a fever and respiratory signs and the infection was confirmed by specific antibodies against сhlamydia detected in their blood and by abnormalities in rabbit kindling. Complement fixation test of paired rabbit sera revealed an increase in the titers of specific antibodies against сhlamydia, which on Day 7 post infection varied within 1:7.5; on Day 14, mean concentration was 1:40 and by Day 30 mean titer increased to 1:60. However, when pathological materials from the urogenital tract of the experimental animals were tested in polymerase chain reaction and in smear microscopy, it was impossible to confirm that there is an etiological link between сhlamydia and kindling problems in experimental animals. At the same time, molecular and genetic tests of internal organs (liver) sampled from stillborn baby rabbits revealed the сhlamydia genome, thus, proving сhlamydia involvement into the pathological kindling. Therefore, such a retrospective method as complement fixation test with a сhlamydia antigen is of high diagnostic value for lifetime сhlamydia diagnosis.
{"title":"Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits","authors":"V. Evstifeev, F. М. Khusainov, S. I. Yakovlev, R. I. Shangaraev, V. I. Eremets","doi":"10.29326/2304-196x-2023-12-3-222-227","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-222-227","url":null,"abstract":"Specific antibodies against сhlamydia were detected using complement fixation test and сhlamydia genome was detected using polymerase chain reaction in pregnant rabbits experimentally infected with Chlamydia psittaci. The infected rabbits developed a fever and respiratory signs and the infection was confirmed by specific antibodies against сhlamydia detected in their blood and by abnormalities in rabbit kindling. Complement fixation test of paired rabbit sera revealed an increase in the titers of specific antibodies against сhlamydia, which on Day 7 post infection varied within 1:7.5; on Day 14, mean concentration was 1:40 and by Day 30 mean titer increased to 1:60. However, when pathological materials from the urogenital tract of the experimental animals were tested in polymerase chain reaction and in smear microscopy, it was impossible to confirm that there is an etiological link between сhlamydia and kindling problems in experimental animals. At the same time, molecular and genetic tests of internal organs (liver) sampled from stillborn baby rabbits revealed the сhlamydia genome, thus, proving сhlamydia involvement into the pathological kindling. Therefore, such a retrospective method as complement fixation test with a сhlamydia antigen is of high diagnostic value for lifetime сhlamydia diagnosis.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"13 29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139343719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-265-272
I. M. Klinovitskaya, М. А. Shibayev, А. К. Karaulov, А. М. Selyanin
Remuneration is one of the main factors that can motivate an employee to work productively, as well as influence the prestige of a profession. The paper presents the results of analysis of remuneration of the veterinary specialists of the State Veterinary Service for the country as a whole, for the federal districts and 85 regions of the country, as well as at various organizational levels within the Veterinary Service for 2021. The assessment of the veterinary specialist salary level as compared with that throughout the economy of the Russian Federation Subjects is presented. The veterinary specialist average monthly salary for the State Veterinary Service as a whole varies within a wide range: from 16.3 to 114.9 thousand rubles. The minimum and maximum salaries at different organizational levels within the State Veterinary Service differ 5–8-fold. The veterinary specialist salary level is lower than that for the economy of the relevant Russian Federation Subject: for the State Veterinary Service as a whole – in 69 regions of the country, for treatment and preventive care institutions – in 71 Subjects, in laboratory diagnosis institutions – in 72 Subjects. Of all the federal districts, the North Caucasian Federal District has the lowest veterinary specialist salary level both in absolute terms (about 21 thousand rubles) and as compared with the average for the economy of the Subjects of this federal district (64%). The paper also examines veterinary specialist modal and median salary values, which allow for the assessment of remuneration of this category of the State Veterinary Service staff from different viewpoints.
{"title":"Remuneration of veterinary specialists of the State Veterinary Service in the Russian Federation Subjects","authors":"I. M. Klinovitskaya, М. А. Shibayev, А. К. Karaulov, А. М. Selyanin","doi":"10.29326/2304-196x-2023-12-3-265-272","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-265-272","url":null,"abstract":"Remuneration is one of the main factors that can motivate an employee to work productively, as well as influence the prestige of a profession. The paper presents the results of analysis of remuneration of the veterinary specialists of the State Veterinary Service for the country as a whole, for the federal districts and 85 regions of the country, as well as at various organizational levels within the Veterinary Service for 2021. The assessment of the veterinary specialist salary level as compared with that throughout the economy of the Russian Federation Subjects is presented. The veterinary specialist average monthly salary for the State Veterinary Service as a whole varies within a wide range: from 16.3 to 114.9 thousand rubles. The minimum and maximum salaries at different organizational levels within the State Veterinary Service differ 5–8-fold. The veterinary specialist salary level is lower than that for the economy of the relevant Russian Federation Subject: for the State Veterinary Service as a whole – in 69 regions of the country, for treatment and preventive care institutions – in 71 Subjects, in laboratory diagnosis institutions – in 72 Subjects. Of all the federal districts, the North Caucasian Federal District has the lowest veterinary specialist salary level both in absolute terms (about 21 thousand rubles) and as compared with the average for the economy of the Subjects of this federal district (64%). The paper also examines veterinary specialist modal and median salary values, which allow for the assessment of remuneration of this category of the State Veterinary Service staff from different viewpoints.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139344709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-259-264
А. Smagulova, Е. V. Kukhar, Т. Glotova, А. G. Glotov
Dermatophytoses are diseases of skin and its accessory structures that are widely spread worldwide. They are most commonly caused by fungi of the genera Micro sporum and Trichophyton. The identification of the agent’s species has a great epidemiological significance and is essential for effective therapy. The aim of the study is the identification and phylogenetic analysis of dermatophytes isolated from dogs and cats in the Republic of Kazakhstan and the Russian Federation by means of molecular techniques. The fungal isolate species were confirmed by sequencing using two rDNA internal transcribed spacer (ITS) primer pairs, and this allowed for their deposition to the GenBank database. Based on the sequencing results, Microsporum canis (12 strains) and Trichophyton benhamiae (2 strains) were identified. The nucleotide sequences were analysed, and phylogenetic trees were constructed, taking into account the results of the dermatophyte identification using two primer pairs. The constructed phylogenetic trees reflecting the relationships of dermatophytes showed that, irrespective of the primer pairs used, the Microsporum and Trichophyton pathogens are in all cases reliably assigned to different clades. The analysis of ITS4F/ITS5R sequence fragment structures enabled the establish- ment of genetic relatedness between the Trichophyton benhamiae strains first isolated from cats in Russia and the Russian strain recovered from a guinea pig. The comparative analysis of the genomes of the Microsporum and Trichophyton fungi and reference strains revealed a relatively low level of intraspecies polymor- phism and point mutations of the sequences. The data analysis demonstrated a high percentage of nucleotide sequence homology, and this allows using the primers for PCR tests intended for dermatophytosis diagnosis in cats and dogs.
{"title":"Phylogenetic analysis of dermatophytes isolated from small domestic animals","authors":"А. Smagulova, Е. V. Kukhar, Т. Glotova, А. G. Glotov","doi":"10.29326/2304-196x-2023-12-3-259-264","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-259-264","url":null,"abstract":"Dermatophytoses are diseases of skin and its accessory structures that are widely spread worldwide. They are most commonly caused by fungi of the genera Micro sporum and Trichophyton. The identification of the agent’s species has a great epidemiological significance and is essential for effective therapy. The aim of the study is the identification and phylogenetic analysis of dermatophytes isolated from dogs and cats in the Republic of Kazakhstan and the Russian Federation by means of molecular techniques. The fungal isolate species were confirmed by sequencing using two rDNA internal transcribed spacer (ITS) primer pairs, and this allowed for their deposition to the GenBank database. Based on the sequencing results, Microsporum canis (12 strains) and Trichophyton benhamiae (2 strains) were identified. The nucleotide sequences were analysed, and phylogenetic trees were constructed, taking into account the results of the dermatophyte identification using two primer pairs. The constructed phylogenetic trees reflecting the relationships of dermatophytes showed that, irrespective of the primer pairs used, the Microsporum and Trichophyton pathogens are in all cases reliably assigned to different clades. The analysis of ITS4F/ITS5R sequence fragment structures enabled the establish- ment of genetic relatedness between the Trichophyton benhamiae strains first isolated from cats in Russia and the Russian strain recovered from a guinea pig. The comparative analysis of the genomes of the Microsporum and Trichophyton fungi and reference strains revealed a relatively low level of intraspecies polymor- phism and point mutations of the sequences. The data analysis demonstrated a high percentage of nucleotide sequence homology, and this allows using the primers for PCR tests intended for dermatophytosis diagnosis in cats and dogs.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139344807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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