Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-228-232
M. Ostyakova, I. Shulga, V. Irkhina, K. S. Kositsyna, N. Golaydo
The effective management practices of dairy farming are inextricably linked with the production of high quality milk, while the mastitis is one of the causes of reductions in milk yields and quality. The aim of the work was to study the microbiological profile of milk, the sensitivity of isolated microorganisms to antibiotics and metabolism features of cows with mastitis in the Amur Oblast. The following microorganisms were identified in the milk samples from cows with mastitis: Staphylococcus epidermidis (34.69%); microbial associations: Streptococcus agalactiae + Escherichia coli (32.65%) and Staphylococcus saprophyticus + Streptococcus agalactiae + Escherichia coli (30.61%); Staphylococcus haemolyticus (2.05%). Microorganisms isolated from the milk of mastitis-affected cows were susceptible to the following antimicrobials: Escherichia coli to cefotaxime (28.00 ± 2.00 mm) and ceftriaxone (27.50 ± 0.35 mm); Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus to cefotaxime (35.50 ± 0.18 mm) and amoxicillin (35.10 ± 0.35 mm); Streptococcus agalactiae to tetracycline (27.60 ± 1.17 mm) and gentamicin (26.40 ± 0.99 mm). Metabolic disorders were observed in cows with mastitis. The albumin-globulin ratio was reduced (0.41), which is typical for various inflammatory processes; and a low albumin levels (29.00 ± 0.89%) suggested a decreased protein synthesis in hepatocytes. A moderate increase in gamma globulins (47.60 ± 1.05%) was associated with stimulation of the phagocytic mononuclear system. Water and mineral metabolism disorders were confirmed by low levels of calcium (1.80 ± 0.03 mmol/L) and magnesium (0.70 ± 0.02 mmol/L), which is a sign of many pathological conditions, and is associated with heavy lactation. Calcium-phosphorus ratio was reduced (0.82). Hematological indicators suggested hypochromic anemia (hemoglobin level – 100.60 ± 1.28 g/L, globular value – 0.60 ± 0.01). The leukogram was indicative of lymphocytopenia (36.90 ± 2.60%) and neutrophilia (rod-shaped neutrophils – 1.80 ± 0.13%, segmented neutrophils – 51.80 ± 2.51%).
{"title":"Metabolism features and milk microbiota of cows with mastitis in the Amur Oblast","authors":"M. Ostyakova, I. Shulga, V. Irkhina, K. S. Kositsyna, N. Golaydo","doi":"10.29326/2304-196x-2023-12-3-228-232","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-228-232","url":null,"abstract":"The effective management practices of dairy farming are inextricably linked with the production of high quality milk, while the mastitis is one of the causes of reductions in milk yields and quality. The aim of the work was to study the microbiological profile of milk, the sensitivity of isolated microorganisms to antibiotics and metabolism features of cows with mastitis in the Amur Oblast. The following microorganisms were identified in the milk samples from cows with mastitis: Staphylococcus epidermidis (34.69%); microbial associations: Streptococcus agalactiae + Escherichia coli (32.65%) and Staphylococcus saprophyticus + Streptococcus agalactiae + Escherichia coli (30.61%); Staphylococcus haemolyticus (2.05%). Microorganisms isolated from the milk of mastitis-affected cows were susceptible to the following antimicrobials: Escherichia coli to cefotaxime (28.00 ± 2.00 mm) and ceftriaxone (27.50 ± 0.35 mm); Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus to cefotaxime (35.50 ± 0.18 mm) and amoxicillin (35.10 ± 0.35 mm); Streptococcus agalactiae to tetracycline (27.60 ± 1.17 mm) and gentamicin (26.40 ± 0.99 mm). Metabolic disorders were observed in cows with mastitis. The albumin-globulin ratio was reduced (0.41), which is typical for various inflammatory processes; and a low albumin levels (29.00 ± 0.89%) suggested a decreased protein synthesis in hepatocytes. A moderate increase in gamma globulins (47.60 ± 1.05%) was associated with stimulation of the phagocytic mononuclear system. Water and mineral metabolism disorders were confirmed by low levels of calcium (1.80 ± 0.03 mmol/L) and magnesium (0.70 ± 0.02 mmol/L), which is a sign of many pathological conditions, and is associated with heavy lactation. Calcium-phosphorus ratio was reduced (0.82). Hematological indicators suggested hypochromic anemia (hemoglobin level – 100.60 ± 1.28 g/L, globular value – 0.60 ± 0.01). The leukogram was indicative of lymphocytopenia (36.90 ± 2.60%) and neutrophilia (rod-shaped neutrophils – 1.80 ± 0.13%, segmented neutrophils – 51.80 ± 2.51%).","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139344737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-197-207
М. I. Doronin, D. V. Mikhalishin, A. V. Sprygin, A. Mazloum, T. Zhbanova, К. N. Gruzdev, Е. V. Chernyshova
Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecular biology. Practical advantage of simplicity as well as combination of high speed, sensitivity and specificity made it possible to use this analysis for nucleic acid quantitation. The paper presents general information and recommended rules for the development of real-time qPCR. The publication is aimed to acquaint the researchers and reviewers with necessary requirements to be followed in order to ensure high accuracy, reliability and transparency of the experiments, correct interpretation and repeatability of the test results. Current approaches are described that allow obtaining reliable and consistent results by different operators, at different times and in different laboratories. Basic requirements for reagents used, nucleotide sequences and validation methods are given. In general, the publication gives the information needed to achieve three ultimate goals: to provide the authors with a broad range of tools and requirements for the development of real-time qPCR based-techniques; to give the possibility to the reviewers and editors of assessing the quality of articles and guidelines/instructions in accordance with the required criteria; to obtain consistent and reliable results of tests performed using this method.
{"title":"Current approaches to development of real-time qPCR test-kits","authors":"М. I. Doronin, D. V. Mikhalishin, A. V. Sprygin, A. Mazloum, T. Zhbanova, К. N. Gruzdev, Е. V. Chernyshova","doi":"10.29326/2304-196x-2023-12-3-197-207","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-197-207","url":null,"abstract":"Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecular biology. Practical advantage of simplicity as well as combination of high speed, sensitivity and specificity made it possible to use this analysis for nucleic acid quantitation. The paper presents general information and recommended rules for the development of real-time qPCR. The publication is aimed to acquaint the researchers and reviewers with necessary requirements to be followed in order to ensure high accuracy, reliability and transparency of the experiments, correct interpretation and repeatability of the test results. Current approaches are described that allow obtaining reliable and consistent results by different operators, at different times and in different laboratories. Basic requirements for reagents used, nucleotide sequences and validation methods are given. In general, the publication gives the information needed to achieve three ultimate goals: to provide the authors with a broad range of tools and requirements for the development of real-time qPCR based-techniques; to give the possibility to the reviewers and editors of assessing the quality of articles and guidelines/instructions in accordance with the required criteria; to obtain consistent and reliable results of tests performed using this method.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139345038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-246-252
N. V. Shanshin
The paper demonstrates how a joint use of polyvalent serum and immunomodulators affects morbidity, survival, and resistance of calves in the early postnatal period. The objectives of the research are: to study how a joint administration of polyvalent serum and immunomodulators changes dynamics of morpho-biochemical and immunological blood parameters in calves; to determine an optimal ratio between the polyvalent serum and immunomodulators and frequency of administration so that to increase overall body resistance, ensure survival of calves in the early postnatal period; to assess cost-effectiveness of the joint use of polyvalent serum and immunomodulators. Research and production testing was done in Simmental calves. For this purpose one control group and five experimental groups were formed (at least 5 animals in each group). Polyvalent serum (20.0 mL) was once administered subcutaneously to the control calves on the first day of life and the animals of the experimental groups received the serum and immunomodulators according to the relevant dosing instructions. The obtained results demonstrate that the optimal protocols include a single administration of “Ribotan” in combination with serum on the first day of life, as well as a double administration of a polyvalent serum 7 days later, alternating “Fosprenil” with “Immunophane”. Administration of polyvalent serum together with an immunomodulator to newborn calves can reduce the morbidity by 70.0% and achieve 100.0% survival, improving this indicator by 22.3% compared to the control. Cost-effectiveness assessment of the veterinary measures specified in these protocols shows that each rouble spent on the measures saves 25.29 roubles. To increase nonspecific resistance of calves in the early postnatal period, preference shall be given to those veterinary medicinal product that can not only normalize the immune system, but also have a combined positive effect on homeostasis in general, stimulate the growth and development of young animals at the early stages of ontogenesis.
{"title":"Joint use of polyvalent serum and immunomodulators for calves in early postnatal period","authors":"N. V. Shanshin","doi":"10.29326/2304-196x-2023-12-3-246-252","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-246-252","url":null,"abstract":"The paper demonstrates how a joint use of polyvalent serum and immunomodulators affects morbidity, survival, and resistance of calves in the early postnatal period. The objectives of the research are: to study how a joint administration of polyvalent serum and immunomodulators changes dynamics of morpho-biochemical and immunological blood parameters in calves; to determine an optimal ratio between the polyvalent serum and immunomodulators and frequency of administration so that to increase overall body resistance, ensure survival of calves in the early postnatal period; to assess cost-effectiveness of the joint use of polyvalent serum and immunomodulators. Research and production testing was done in Simmental calves. For this purpose one control group and five experimental groups were formed (at least 5 animals in each group). Polyvalent serum (20.0 mL) was once administered subcutaneously to the control calves on the first day of life and the animals of the experimental groups received the serum and immunomodulators according to the relevant dosing instructions. The obtained results demonstrate that the optimal protocols include a single administration of “Ribotan” in combination with serum on the first day of life, as well as a double administration of a polyvalent serum 7 days later, alternating “Fosprenil” with “Immunophane”. Administration of polyvalent serum together with an immunomodulator to newborn calves can reduce the morbidity by 70.0% and achieve 100.0% survival, improving this indicator by 22.3% compared to the control. Cost-effectiveness assessment of the veterinary measures specified in these protocols shows that each rouble spent on the measures saves 25.29 roubles. To increase nonspecific resistance of calves in the early postnatal period, preference shall be given to those veterinary medicinal product that can not only normalize the immune system, but also have a combined positive effect on homeostasis in general, stimulate the growth and development of young animals at the early stages of ontogenesis.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-253-258
S. I. Suleymanov
Despite numerous studies, the problem of escherichiosis in newborn calves remains one of the most urgent due to the extensive spread of the disease and high mortality of young animals. This paper presents results of experimental studies carried out at the FSBSI “All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy”. The aim of the work was to conduct complex morphological studies using modern methods for a deeper understanding of escherichiosis etiology and pathogenesis. For that, pathological samples were collected from 28 1–10 day-old calves diagnosed with colibacteriosis at the initial disease stage, demonstrating pronounced clinical signs and having the terminal stage of the disease. Samples from 6 clinically healthy calves of a similar age were used as control. It was found that newborn calves at the initial stage of escherichiosis demonstrated early structural changes in the ultrastructure of mucosa cells of the rennet and small intestine, as well as in the liver parenchyma, and mild changes – in the exocrine part of the pancreas. The most profound morphofunctional changes were observed in digestive organs with apparent clinical signs of the disease. As the condition developed, the range of pathological processes expanded and involved the structural organization of the rennet, small and large intestines, liver and pancreas. At the terminal stage of escherichiosis, deep inflammatory processes occurred not only in digestive organs, but also in other systems of diseased calves. Structural changes in digestive organs had an alterative nature at the initial disease stage, whereas in case of clinically pronounced disease signs there were manifestations of catarrhal-necrotic inflammation with multiple hemorrhages in the gastrointestinal tract and parenchymal organs. Digestive organ pathology plays the leading role in formation of the clinical and morphological picture at the initial stage of escherichiosis in calves. As the disease developed, the calves demonstrated changes at molecular and subcellular levels that were detected using histochemical and ultrastructural tests.
{"title":"Functional morphology of digestive organs of newborn calves and pathogenesis of escherichiosis","authors":"S. I. Suleymanov","doi":"10.29326/2304-196x-2023-12-3-253-258","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-253-258","url":null,"abstract":"Despite numerous studies, the problem of escherichiosis in newborn calves remains one of the most urgent due to the extensive spread of the disease and high mortality of young animals. This paper presents results of experimental studies carried out at the FSBSI “All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy”. The aim of the work was to conduct complex morphological studies using modern methods for a deeper understanding of escherichiosis etiology and pathogenesis. For that, pathological samples were collected from 28 1–10 day-old calves diagnosed with colibacteriosis at the initial disease stage, demonstrating pronounced clinical signs and having the terminal stage of the disease. Samples from 6 clinically healthy calves of a similar age were used as control. It was found that newborn calves at the initial stage of escherichiosis demonstrated early structural changes in the ultrastructure of mucosa cells of the rennet and small intestine, as well as in the liver parenchyma, and mild changes – in the exocrine part of the pancreas. The most profound morphofunctional changes were observed in digestive organs with apparent clinical signs of the disease. As the condition developed, the range of pathological processes expanded and involved the structural organization of the rennet, small and large intestines, liver and pancreas. At the terminal stage of escherichiosis, deep inflammatory processes occurred not only in digestive organs, but also in other systems of diseased calves. Structural changes in digestive organs had an alterative nature at the initial disease stage, whereas in case of clinically pronounced disease signs there were manifestations of catarrhal-necrotic inflammation with multiple hemorrhages in the gastrointestinal tract and parenchymal organs. Digestive organ pathology plays the leading role in formation of the clinical and morphological picture at the initial stage of escherichiosis in calves. As the disease developed, the calves demonstrated changes at molecular and subcellular levels that were detected using histochemical and ultrastructural tests.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139344367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.29326/2304-196x-2023-12-3-233-239
V. Kirpichenko, F. A. Bakieva, S. B. Mamanova, Е. К. Ospanov, S. Е. Kaimoldina
A comprehensive serological monitoring is currently underway in the Republic of Kazakhstan to detect the circulation of the infectious bovine rhinotracheitis pathogen. To conduct a full-fledged and resultful study, the principles of sampling size representativeness provision and mathematical calculations were observed. The sampling size of the total number of epizootological units included mainly the raions and settlements in which (or near which) infectious bovine rhinotracheitis cases had been previously recorded. The sampling size of livestock population included in the study was determined in accordance with the recommendations of the World Organization for Animal Health. Thus, the study covered 7 (out of 13) raions of the Karaganda Oblast in 2021 and 2022. The other 6 raions and cities of regional significance will be included in the research in 2023. The paper presents the results of infectious bovine rhinotracheitis retrospective analysis and own studies conducted in 2021–2022. Statistical analysis and graphical visualization of investigation results were performed using Statistica, Excel, and QGIS programs. It was established that the epizootic situation for this disease was unfavourable in the Karaganda Oblast in 2021–2022. The data and results of serological studies presented by the Veterinary Control and Surveillance Committee of the Ministry of Agriculture of the Republic of Kazakhstan confirm the circulation of the infectious bovine rhinotracheitis virus in the following raions of the Karaganda Oblast: Abaisky, Aktogaisky, Bukhar-Zhyrausky, Karkaralinsky, Nurinsky and Osakarovsky.
{"title":"Epizootic situation for infectious bovine rhinotracheitis in the Karaganda Oblast, the Republic of Kazakhstan, in 2021–2022","authors":"V. Kirpichenko, F. A. Bakieva, S. B. Mamanova, Е. К. Ospanov, S. Е. Kaimoldina","doi":"10.29326/2304-196x-2023-12-3-233-239","DOIUrl":"https://doi.org/10.29326/2304-196x-2023-12-3-233-239","url":null,"abstract":"A comprehensive serological monitoring is currently underway in the Republic of Kazakhstan to detect the circulation of the infectious bovine rhinotracheitis pathogen. To conduct a full-fledged and resultful study, the principles of sampling size representativeness provision and mathematical calculations were observed. The sampling size of the total number of epizootological units included mainly the raions and settlements in which (or near which) infectious bovine rhinotracheitis cases had been previously recorded. The sampling size of livestock population included in the study was determined in accordance with the recommendations of the World Organization for Animal Health. Thus, the study covered 7 (out of 13) raions of the Karaganda Oblast in 2021 and 2022. The other 6 raions and cities of regional significance will be included in the research in 2023. The paper presents the results of infectious bovine rhinotracheitis retrospective analysis and own studies conducted in 2021–2022. Statistical analysis and graphical visualization of investigation results were performed using Statistica, Excel, and QGIS programs. It was established that the epizootic situation for this disease was unfavourable in the Karaganda Oblast in 2021–2022. The data and results of serological studies presented by the Veterinary Control and Surveillance Committee of the Ministry of Agriculture of the Republic of Kazakhstan confirm the circulation of the infectious bovine rhinotracheitis virus in the following raions of the Karaganda Oblast: Abaisky, Aktogaisky, Bukhar-Zhyrausky, Karkaralinsky, Nurinsky and Osakarovsky.","PeriodicalId":507311,"journal":{"name":"Veterinary Science Today","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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