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Coupling Michelson-like lateral shear interferometric microscopy with self-referencing numerical phase calibration for quantitative measurement of 3D surface morphology of biological cells 将迈克尔逊式横向剪切干涉显微镜与自参照数值相位校准联用,定量测量生物细胞的三维表面形态
Pub Date : 2024-04-16 DOI: 10.2351/7.0001360
Tengfei Sun, Shaoying Ke, Wentao Sui, Wenhao Zhang, Peng Lu, Dongfeng Qi, Bing Yang, Juan Wei, Wei Zhang, Hongyu Zheng
A Michelson interferometer is commonly used for evaluating the morphology of a cell. However, the interference imaging with reference and object beams is easily affected by external vibrations and environmental disturbances, leading to unstable interference patterns. In this paper, the three-dimensional surface morphology of the biological cell is evaluated by a new quantitative phase imaging method, which couples Michelson-like lateral shear interferometric microscopy with self-referencing numerical phase calibration. The Michelson-like lateral shear interferometric microscopy is constructed by replacing the two plane mirrors of the traditional Michelson interferometer with two common right-angle prisms and generates interference fringe patterns. The lateral shear is created and freely adjustable by simply translating/or rotating one right-angle prism. To calculate the phase information of the biological cells quantitatively, the classical Fourier transform method is used to process the recorded interferogram, and then the self-referencing numerical phase calibration method is utilized for acquiring accurate phase information. Successfully achieving quantitative phase imaging of a cell verifies the feasibility and practicability of the proposed method.
迈克尔逊干涉仪通常用于评估细胞的形态。然而,参考光束和目标光束的干涉成像容易受到外部振动和环境干扰的影响,导致干涉图不稳定。本文采用一种新的定量相位成像方法来评估生物细胞的三维表面形态,该方法将迈克尔逊类横向剪切干涉显微镜与自参考数值相位校准相结合。米克尔逊式横向剪切干涉显微镜是用两个普通直角棱镜取代传统米克尔逊干涉仪的两个平面镜,并产生干涉条纹图案。只需平移/或旋转一个直角棱镜,即可产生横向剪切并自由调节。为了定量计算生物细胞的相位信息,采用经典的傅里叶变换方法处理记录的干涉图,然后利用自参照数值相位校准方法获取准确的相位信息。成功实现细胞的定量相位成像验证了所提方法的可行性和实用性。
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引用次数: 0
Research on melting behavior of coaxially fed powder in diode laser cladding process 二极管激光熔覆工艺中同轴送粉熔化行为的研究
Pub Date : 2024-04-11 DOI: 10.2351/7.0001047
Zhu Ming, Hanlin Yan, Zongzhi Zhang, Yang Qian, Shi Yu, Fan Ding
The purpose of this study was to investigate the thermal interaction between coaxially fed powder and diode laser, which is extremely complex and difficult to be detected in thermal real-time monitoring by the infrared camera system. In order to analyze the dynamic melting behavior of the powder, a high-speed camera system was established to capture the melting behavior of a single powder coaxially fed into the laser, which can reflect the entire melting process. There is a transition of “solid → solid-liquid two-phase state → liquid state” after a single powder enters the laser, and the duration and thermophysical behavior in different transition stages are not the same. Different states and duration determine distinct heat absorption effects. Based on high-speed camera detection results, the thermophysical behavior in different characteristic stages of the melting process in single powder was studied to develop a mathematical model, which is able to simulate and predict the temperature and state of the powder about to enter the melting pool. The experimental and simulation results show that (1) for a well-tested powder melting process, where the laser power ranges from 100 to 1500 W, the duration of the powder particle's absorption stage is between 4.41 and 18 ms, the duration of the solid-liquid two-phase state is between 0.52 and 2.63 ms, and the duration of the liquid state is between 4.67 and 13.48 ms. (2) The laser power ranges from 100 to 1500 W, and the temperature of the powder particles as they enter the melt pool ranges from 745 to 3200 °C.
本研究的目的是研究同轴送入的粉末与二极管激光器之间的热相互作用,这种相互作用极其复杂,在热实时监测中很难通过红外摄像系统检测到。为了分析粉末的动态熔化行为,建立了一个高速摄像系统,以捕捉同轴送入激光器的单个粉末的熔化行为,从而反映整个熔化过程。单个粉末进入激光器后存在 "固态→固液两相态→液态 "的过渡,不同过渡阶段的持续时间和热物理性质并不相同。不同的状态和持续时间决定了不同的吸热效果。基于高速摄像检测结果,研究了单个粉末在熔化过程中不同特征阶段的热物理行为,从而建立了一个数学模型,该模型能够模拟和预测即将进入熔池的粉末的温度和状态。实验和模拟结果表明:(1) 在激光功率为 100 至 1500 W 的情况下,粉末颗粒吸收阶段的持续时间为 4.41 至 18 ms,固液两相状态的持续时间为 0.52 至 2.63 ms,液态的持续时间为 4.67 至 13.48 ms。(2) 激光功率范围为 100 至 1500 W,粉末颗粒进入熔池时的温度范围为 745 至 3200 ℃。
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引用次数: 0
Laser writing of memristive logic gates and crossbar arrays 激光写入忆阻器逻辑门和交叉条阵列
Pub Date : 2024-04-10 DOI: 10.2351/7.0001345
Joshua Jones, Ningyue Mao, Peng Peng
Memristor-based logic circuits are gaining a lot of attention due to the potential for high logic density hardware and novel in-memory computing applications. Readily available methods for fabricating of memristor logic structures that are suitable for integration with conventional computer hardware are a growing need. This work presents a direct laser writing process capable of rapidly fabricating memristor logic circuits by laser irradiation of metal salt precursor solutions. Planar memristor patterns are fabricated, and their I–V response is characterized. Boolean logic gates are fabricated from planar memristor pairs that exhibit low programming voltages and rapid switching. Cu/Cu2O/Cu and Ag/Cu2O/Cu memristors are also fabricated in crossbar arrays, showing the ability to be programmed to multiple resistance states through ultrashort voltage pulses. The devices also show the potential to have high endurance and nonvolatile resistance state retention.
基于忆阻器的逻辑电路因其在高逻辑密度硬件和新型内存计算应用方面的潜力而备受关注。人们越来越需要能与传统计算机硬件集成的现成的忆阻器逻辑结构制造方法。这项研究提出了一种直接激光写入工艺,能够通过激光照射金属盐前驱体溶液快速制造忆阻器逻辑电路。我们制作了平面忆阻器图案,并对其 I-V 响应进行了表征。利用平面忆阻器对制作出的布尔逻辑门具有编程电压低、开关速度快的特点。Cu/Cu2O/Cu 和 Ag/Cu2O/Cu 记忆晶闸管也被制作成横条阵列,显示出通过超短电压脉冲编程到多种电阻状态的能力。这些器件还显示出具有高耐用性和非易失性电阻状态保持的潜力。
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引用次数: 0
Characterization of the deformation behavior and microstructure evolution in laser bending of TC4 titanium alloy heavy plate TC4 钛合金厚板在激光弯曲过程中的变形行为和微观结构演变特征
Pub Date : 2024-03-01 DOI: 10.2351/7.0001303
Yulin Shao, Zhanzhou Liu, Haochen Ding, Chi Zhang, Zhiwen Shao, Liwen Zhang
A new plastic processing method for laser bending of TC4 titanium alloy heavy plates was developed in this work. Different from previous studies, the thickness of the plates in this work reached 6 and 12 mm. High-energy laser beam was applied on the surface of the TC4 titanium alloy heavy plate, resulting in thermal stress on the surface of the plate and causing bending deformation. The microstructure and mechanical properties of the bended plates were investigated. A finite element model was also developed to simulate the laser-assisted bending process of the TC4 titanium alloy heavy plate. The microstructure observation indicates that the original α + β worm-like microstructure changed to basket-weave microstructure composed of α′ phase of acicular martensite after laser bending. This microstructure with high densities of dislocation and twinning played an important role in grain boundary strengthening. Therefore, the hardness of the center of the heat-affected zone > the hardness of the base metal > the hardness of the edge of the heat-affected zone after bending. The tensile strength of the heat-affected zone is not significantly different from that of the base metal, but the tensile elongation is slightly lower than that of the base metal and its plasticity is lower. The simulation implies that temperature gradients in the normal direction caused by laser scanning can stimulate a horizontal partial stress σx. The variation of σx causes the plate form a bending angle after laser scanning.
本研究开发了一种用于激光弯曲 TC4 钛合金厚板的新型塑性加工方法。与以往的研究不同,本研究中的板材厚度达到了 6 毫米和 12 毫米。在 TC4 钛合金厚板表面施加高能激光束,使板材表面产生热应力,引起弯曲变形。研究了弯曲板的微观结构和机械性能。此外,还建立了有限元模型来模拟 TC4 钛合金厚板的激光辅助弯曲过程。微观结构观察表明,激光弯曲后,原来的 α + β 蠕虫状微观结构转变为由针状马氏体的 α′ 相组成的篮织状微观结构。这种具有高位错密度和孪晶的微观组织在晶界强化中发挥了重要作用。因此,弯曲后热影响区中心的硬度>基体金属的硬度>热影响区边缘的硬度。热影响区的抗拉强度与基体金属的抗拉强度相差不大,但拉伸伸长率略低于基体金属,塑性也较低。模拟结果表明,激光扫描引起的法线方向的温度梯度会激发水平分应力 σx。σx 的变化会导致板材在激光扫描后形成弯曲角度。
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引用次数: 0
Effect of ultrasonic vibration on microstructural evolution, clad defects, and surface properties in laser direct energy deposition of Inconel 625 超声波振动对激光直接能量沉积铬镍铁合金 625 的微结构演变、熔覆缺陷和表面性能的影响
Pub Date : 2024-03-01 DOI: 10.2351/7.0001258
Biplab Kumar Dash, S. Bhatnagar, Hari Srinivasa Rao Magham, Shubham Rao, Gopinath Muvvala, S. Mullick
Laser direct energy deposition (DED) has some accompanying issues, such as existence of micropores, elemental segregation at grain boundaries, intergranular corrosion, etc. Therefore, the current work aims for a reduction in clad defects and enhancement in surface properties for laser direct deposition of Inconel 625 by implementing ultrasonic vibration. The acoustic streaming and cavitation effect induced by ultrasonic vibration results in the breaking of columnar grains, along with grain refinement and better elemental distribution in the matrix during the solidification process. The investigation is carried out for deposition using a 240 W Yb-fiber laser under the application of ultrasonic vibration with a variable amplitude of 6–13 μm (frequency: 33–28 kHz). A relatively higher vibration amplitude was found more efficient in converting long columnar grains into finer and uniformly distributed equiaxed grains, with a significant reduction in micropores. Further, it resulted in a shorter molten pool lifetime because of the generation of more nucleation centers, leading to better cooling. The above effects resulted in higher microhardness of the deposited layer. Further, the wear and corrosion resistance showed an improvement with the application of vibration, which may be due to the finer equiaxed grains, less porosity, and better elemental distribution at a higher vibration amplitude.
激光直接能量沉积(DED)有一些伴随问题,如存在微孔、晶界元素偏析、晶间腐蚀等。因此,目前的工作旨在通过超声波振动来减少 Inconel 625 激光直接沉积的熔覆缺陷并提高其表面性能。在凝固过程中,超声波振动诱导的声流和空化效应会导致柱状晶粒破碎、晶粒细化以及基体中元素分布的改善。研究使用 240 W Yb 光纤激光器,在振幅为 6-13 μm(频率:33-28 kHz)的超声波振动下进行沉积。结果发现,相对较高的振幅能更有效地将长柱状晶粒转化为更细且分布均匀的等轴晶粒,同时显著减少微孔。此外,由于产生了更多的成核中心,因此熔池寿命更短,冷却效果更好。上述效应使沉积层的显微硬度更高。此外,耐磨性和耐腐蚀性也随着振动的应用而得到改善,这可能是由于在较高的振动振幅下,等轴晶更细、孔隙率更小、元素分布更均匀。
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引用次数: 0
Study on the processing parameter window for laser cleaning thermal barrier coating 激光清洗热障涂层的加工参数窗口研究
Pub Date : 2024-02-01 DOI: 10.2351/7.0001202
Guoqiang Dai, Yunxia Ye, Yinqun Hua
In this paper, laser cleaning of thermal barrier coatings is investigated by finite element simulation and experiments. The effect of different parameters on the maximum temperature of the substrate surface during the cleaning process is studied. The results show that the repetition frequency and pulse width have little effect on the maximum temperature of the substrate surface, and the maximum temperature of the substrate surface has an approximately linear relationship with the laser power and an exponential decay relationship with the scanning speed. From the perspective of cleaning efficiency and damage to the substrate, the parameter windows for laser cleaning of thermal barrier coatings were obtained. The highest cleaning efficiency for the top ceramic layer is about 3.02 mm3/s. The highest cleaning efficiency for the bond layer is about 2.17 mm3/s.
本文通过有限元模拟和实验研究了热障涂层的激光清洗。研究了清洗过程中不同参数对基材表面最高温度的影响。结果表明,重复频率和脉冲宽度对基材表面的最高温度影响不大,基材表面的最高温度与激光功率呈近似线性关系,与扫描速度呈指数衰减关系。从清洗效率和对基底的损伤角度出发,得到了激光清洗隔热涂层的参数窗口。顶部陶瓷层的最高清洁效率约为 3.02 mm3/s。结合层的最高清洁效率约为 2.17 mm3/s。
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引用次数: 0
Pressure estimation via measurement of reduced light scattering coefficient by oblique laser incident reflectometry 通过斜激光入射反射测量法测量减弱的光散射系数来估算压力
Pub Date : 2024-02-01 DOI: 10.2351/7.0001263
David Abookasis, Daniel Malchi, Dror Robinson, Mustafa Yassin
Continuous measurement of pressure is vital in many fields of industry, medicine, and science. Of particular interest is the ability to measure pressure in a noninvasive and contact-free manner. This work presents the potential of oblique incident reflectometry (OIR) to monitor variation in pressure via the reduced scattering parameter (μs′). Pressure deforms the geometry of the medium and causes distortion of its internal structure and the spatial distribution of optical properties. Light scattering is related to the morphology (size, density, distribution, etc.) and refractive index distributions of the medium, and applied pressure will influence directly these parameters. Therefore, we assume that pressure can be quantitatively assessed through monitoring the reduced scattering coefficient. For this purpose, the technique of OIR to evaluate the scattering parameter during pressure variations was utilized. OIR is a simple noninvasive and contact-free imaging technique able to quantify both absorption and scattering properties of a sample. In our setup, the medium is illuminated obliquely by a narrow laser beam, and the diffuse reflectance light is captured by a CCD camera. In offline processing, the shift (δ) of the diffuse light center from the incident point is mathematically analyzed and μs′ coefficient (μs′∼δ−1) is extracted. We present here confirmation of the validity of this assumption through results of a series of experiments performed on turbid liquid and artery occlusion of a human subject under different pressure levels. Thus, μs′ has the potential to serve as a good indicator for the monitoring of pressure.
压力的连续测量在工业、医学和科学的许多领域都至关重要。其中,以非侵入式和非接触方式测量压力的能力尤为重要。这项工作展示了斜入射反射仪 (OIR) 通过减小散射参数 (μs′)来监测压力变化的潜力。压力会使介质的几何形状发生变形,并导致其内部结构和光学特性的空间分布发生扭曲。光散射与介质的形态(大小、密度、分布等)和折射率分布有关,施加的压力将直接影响这些参数。因此,我们认为可以通过监测降低的散射系数来定量评估压力。为此,我们采用了 OIR 技术来评估压力变化时的散射参数。OIR 是一种简单的非侵入式非接触成像技术,能够量化样品的吸收和散射特性。在我们的装置中,介质被一束窄激光斜射,漫反射光被 CCD 相机捕获。在离线处理过程中,对漫射光中心与入射点的偏移 (δ)进行数学分析,并提取出 μs′ 系数 (μs′∼δ-1)。在此,我们通过在不同压力水平下对浑浊液体和人体动脉闭塞进行的一系列实验结果,证实了这一假设的正确性。因此,μs′有可能成为监测压力的良好指标。
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引用次数: 0
Study on the processing parameter window for laser cleaning thermal barrier coating 激光清洗热障涂层的加工参数窗口研究
Pub Date : 2024-02-01 DOI: 10.2351/7.0001202
Guoqiang Dai, Yunxia Ye, Yinqun Hua
In this paper, laser cleaning of thermal barrier coatings is investigated by finite element simulation and experiments. The effect of different parameters on the maximum temperature of the substrate surface during the cleaning process is studied. The results show that the repetition frequency and pulse width have little effect on the maximum temperature of the substrate surface, and the maximum temperature of the substrate surface has an approximately linear relationship with the laser power and an exponential decay relationship with the scanning speed. From the perspective of cleaning efficiency and damage to the substrate, the parameter windows for laser cleaning of thermal barrier coatings were obtained. The highest cleaning efficiency for the top ceramic layer is about 3.02 mm3/s. The highest cleaning efficiency for the bond layer is about 2.17 mm3/s.
本文通过有限元模拟和实验研究了热障涂层的激光清洗。研究了清洗过程中不同参数对基材表面最高温度的影响。结果表明,重复频率和脉冲宽度对基材表面的最高温度影响不大,基材表面的最高温度与激光功率呈近似线性关系,与扫描速度呈指数衰减关系。从清洗效率和对基底的损伤角度出发,得到了激光清洗隔热涂层的参数窗口。顶部陶瓷层的最高清洁效率约为 3.02 mm3/s。结合层的最高清洁效率约为 2.17 mm3/s。
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引用次数: 0
Underwater laser welding of tube-plate structure of aluminum alloy 铝合金管板结构的水下激光焊接
Pub Date : 2024-01-10 DOI: 10.2351/7.0001193
Qi Cheng, Ning Guo, Yunlong Fu, Junhui Tong, Xin Zhang, Hao Chen, Jinlong He
Underwater local dry laser welding of the tube-plate structure was performed first. The effects of laser power and welding speed on welding quality were studied. With the increase in the laser power or decrease in the welding speed, the melting zone (MZ) area and depth-width ratio of the welded joint increased, and metallurgical porosity also increased. When the laser power was 2 kW and the welding speed was 12 mm/s, a better metallurgical bond could be formed between the tube and plate, and there were fewer metallurgical pores in the welded joint. Due to a large amount of evaporation and ionization of water in the gap between the tube and plate, metallurgical pores formed in the welded joint. By adding a waterproof layer on the back of the substrate, the metallurgical porosity decreased from 2.1% to 0%. In order to investigate the influence of a water environment on welding quality, the in-air laser welding was performed. Compared to in-air welded joint, the MZ area, depth to width ratio, and grain size were smaller. The average microhardness of underwater welded joint was higher than that of in-air welded joint due to the refined crystalline strengthening. In addition, because the magnesium burning loss in an underwater environment was less than that in an in-air environment, the microhardness values at the top of the underwater MZ were similar to those at the bottom, while the microhardness values at the top and bottom of the in-air MZ were much different.
首先对管板结构进行了水下局部干激光焊接。研究了激光功率和焊接速度对焊接质量的影响。随着激光功率的增加或焊接速度的降低,焊接接头的熔化区(MZ)面积和深宽比增大,冶金气孔率也增大。当激光功率为 2 kW、焊接速度为 12 mm/s 时,管材和板材之间能形成较好的冶金结合,焊点中的冶金气孔也较少。由于管和板之间的间隙中有大量水分蒸发和电离,焊点中形成了冶金气孔。通过在基板背面添加防水层,冶金孔隙率从 2.1% 降至 0%。为了研究水环境对焊接质量的影响,进行了空气中激光焊接。与空气中的焊点相比,MZ 面积、深宽比和晶粒尺寸都较小。由于结晶强化细化,水下焊接接头的平均显微硬度高于空气中的焊接接头。此外,由于水下环境中的镁燃烧损耗小于空气中的镁燃烧损耗,水下 MZ 顶部的显微硬度值与底部的显微硬度值相似,而空气中 MZ 顶部和底部的显微硬度值相差很大。
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引用次数: 0
Formation and effect of rough surface in pulsed laser removal of thick paint layer on 2024 aluminum 脉冲激光去除 2024 铝材上厚漆层时粗糙表面的形成和影响
Pub Date : 2024-01-09 DOI: 10.2351/7.0001010
Ming Zhu, Hao Zhang, Kun Shi, Xiaofei Hou, Yu Shi, Ding Fan, Wenzhu Zhang
When using pulsed laser to remove a thick paint layer from a 2024 aluminum alloy substrate, the paint layer should be removed layer by layer, and the substrate should remain intact after the paint layer is completely removed. In this process, the formation of a rough surface on the paint increases laser absorption, changing the removal mechanism, which becomes an unstable factor for nondestructive paint removal. This study investigated the laser paint removal mechanism of fluorocarbon paint and found that when the laser fluence was 0.91 J/cm2, the main removal mechanisms were thermal stress and combustion-decomposition removal, while at 94.36 J/cm2, the main mechanism was sputtering removal. The study also investigated the relationship between surface roughness and the number of laser scans, which showed a positive linear correlation. Additionally, an equivalent model of a rough surface was established to analyze the changes in absorption, though this model has yet to be verified and further research and validation is required to confirm our findings.
在使用脉冲激光去除 2024 铝合金基材上的厚漆层时,应逐层去除漆层,并在漆层完全去除后保持基材完好无损。在此过程中,油漆表面形成的粗糙面会增加激光吸收,改变去除机理,成为无损去除油漆的不稳定因素。本研究对氟碳漆的激光除漆机理进行了研究,发现当激光通量为 0.91 J/cm2 时,主要的除漆机理是热应力除漆和燃烧分解除漆,而当激光通量为 94.36 J/cm2 时,主要的除漆机理是溅射除漆。研究还调查了表面粗糙度与激光扫描次数之间的关系,结果显示两者呈正线性关系。此外,还建立了粗糙表面的等效模型来分析吸收的变化,不过该模型还有待验证,需要进一步的研究和验证来证实我们的发现。
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引用次数: 0
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Journal of Laser Applications
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