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Metabolomic Profiling, Volatile Fatty Acids, and Greenhouse Gas Emissions of Beef Cattle Infused with Different Essential Oil Blends 不同精油混合物浸泡肉牛的代谢组学分析、挥发性脂肪酸和温室气体排放
Pub Date : 2024-07-23 DOI: 10.3390/ruminants4030024
Deborah O. Okedoyin, Joel O. Alabi, Chika C. Anotaenwere, Michael Wuaku, DeAndrea Gray, Oludotun O. Adelusi, K. Ike, P. Dele, O. Oderinwale, M. Idowu, I. Ogunade, U. Anele
Essential oils are natural feed additives that improve animal health and enhance their performance. This study investigated the effects of the rumen infusion of five essential oil blends (EOBs) on greenhouse gas (GHG) emissions, rumen fermentation parameters, and rumen metabolome and metabolic pathways in Black Angus cows. Using a 6 × 6 Latin Square experimental design, a 90-day study was conducted with six cattle. A daily dosage of 4 mL of EOBs was administered during each infusion. Volcano plot analyses between the control (CON) and each of the EOBs (EOB1, EOB2, EOB3, EOB4, and EOB5) revealed several differentially abundant (p ≤ 0.05; absolute fold change ≥1.5) metabolites. The EOB5 treatment exhibited the most significant impact, with 26 differentially abundant metabolites, including elevated valine and reduced gallic acid. Volatile fatty acids (VFAs), including valerate, isobutyrate, and isovalerate, were significantly increased (p < 0.05). GHG emissions were not significantly affected, but a numerical decrease was observed in the animals infused with the EOB5 treatment. Ammonia nitrogen concentrations remained within the suitable range for rumen microbes’ growth, indicating a normal internal environment for microbial crude protein synthesis. In conclusion, the study has demonstrated that the direct infusion of EOBs significantly improved the generation of VFAs and impacted the energy production, protein synthesis, and microbial activity of the animals.
精油是一种天然饲料添加剂,可改善动物健康并提高其生产性能。本研究调查了在黑安格斯奶牛瘤胃中注入五种混合精油(EOB)对温室气体(GHG)排放、瘤胃发酵参数、瘤胃代谢组和代谢途径的影响。采用 6 × 6 拉丁方实验设计,对 6 头牛进行了为期 90 天的研究。在每次输注过程中,每天输注 4 毫升 EOBs。对照组(CON)和每种 EOBs(EOB1、EOB2、EOB3、EOB4 和 EOB5)之间的火山图分析表明了几种含量不同(p ≤ 0.05;绝对折叠变化≥1.5)的代谢物。EOB5 处理的影响最为显著,有 26 种代谢物含量不同,包括缬氨酸增加和没食子酸减少。包括戊酸、异丁酸和异戊酸在内的挥发性脂肪酸(VFAs)显著增加(p < 0.05)。温室气体排放未受到明显影响,但在输注 EOB5 处理剂的动物中观察到数值下降。氨氮浓度保持在适合瘤胃微生物生长的范围内,表明微生物粗蛋白合成的内部环境正常。总之,该研究表明,直接灌注 EOB 能显著改善 VFAs 的产生,并影响动物的能量生产、蛋白质合成和微生物活性。
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引用次数: 0
The Effects of Breed, Lactation Number, and Lameness on the Behavior, Production, and Reproduction of Lactating Dairy Cows in Central Texas 品种、泌乳数和跛足对得克萨斯州中部泌乳奶牛行为、生产和繁殖的影响
Pub Date : 2024-07-12 DOI: 10.3390/ruminants4030023
Lily A. Martin, Edward C. Webb, C. L. Runyan, Jennifer A. Spencer, Barbara W. Jones, Kimberly B. Wellmann
The objective of this study was to evaluate the effects of breed, lactation number, and lameness on lying time, milk yield, milk urea nitrogen concentration (MUN), progesterone concentration (P4), and the calving-to-conception interval (CCI) of lactating dairy cows in Central Texas. A total of 84 lactating dairy cows (Holsteins, Jerseys, and crossbreeds) from a commercial dairy farm in Central Texas were randomly selected and enrolled in this study from October 2023 to February 2024. Cows (60 ± 7 DIM) were enrolled in cohorts weekly for five weeks and were randomly fitted with an IceQube pedometer (IceRobotics, Edinburgh, UK) to track lying time. Lameness and body condition scores (BCS) were recorded, and blood samples were collected once a week. Parameters of reproductive performance included insemination rate, conception rate, pregnancy rate, and the CCI. Monthly dairy herd improvement association (DHIA) testing included milk yield and MUN concentrations. Breed and lactation number had a significant effect on milk yield, MUN concentration, lying time, BCS, and lameness (p < 0.001). Lactation number had a significant effect on P4 concentrations (p < 0.001). There was a positive correlation between lameness and milk yield (p = 0.014) and a negative correlation between lameness and MUN concentrations (p = 0.038).
本研究的目的是评估品种、泌乳数和跛足对得克萨斯州中部泌乳奶牛的卧床时间、产奶量、牛奶尿素氮浓度(MUN)、孕酮浓度(P4)和产犊至受孕间隔(CCI)的影响。从 2023 年 10 月到 2024 年 2 月,德克萨斯州中部一家商业化奶牛场共随机选取了 84 头泌乳奶牛(荷斯坦、杰西和杂交品种)参与本研究。奶牛(60 ± 7 DIM)每周分批饲养五周,并随机安装 IceQube 计步器(IceRobotics,英国爱丁堡)以追踪卧地时间。记录跛行和体况评分(BCS),每周采集一次血样。繁殖性能参数包括授精率、受胎率、妊娠率和 CCI。乳牛群改良协会(DHIA)的月度检测包括产奶量和MUN浓度。品种和泌乳数对产奶量、MUN浓度、卧位时间、BCS和跛行有显著影响(p < 0.001)。泌乳次数对 P4 浓度有显著影响(p < 0.001)。跛足与产奶量呈正相关(p = 0.014),跛足与 MUN 浓度呈负相关(p = 0.038)。
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引用次数: 0
Evaluating the Bias of Two Point-of-Care Glucometers for Calves and Ewes: Awareness for Ruminant Practitioners 评估用于犊牛和母羊的两种定点血糖仪的偏差:反刍动物从业人员的认识
Pub Date : 2024-07-02 DOI: 10.3390/ruminants4030022
Ryan Flynn, Haley Cremerius, Lisa Ebner, P. Mulon, Jessica Garcia, Kailee Bennett, Jessica Gerbert, Lainey Harvill, Olivia G Escher, Channing Cantrell, Windy Soto-Gonzalez, Rebecca R. Rahn, Jeff D. Olivarez, L. Yuan, J. Mochel, A. Kreuder, Joe Smith
(1) Background: Multiple point-of-care (POC) glucometers are in use in veterinary medicine, but few are compared to each other. This leaves the potential for clinicians to be unaware of the effect of bias when comparing results from different POC glucometers. (2) Methods: Samples from healthy calves and ewes were simultaneously compared with two POC veterinary glucometers, the Precision Xtra and the AlphaTrak2, under both the “canine” and “feline” settings. The results of each sample were statistically analyzed with linear regression and Bland–Altman analysis. (3) Results: 170 samples from healthy calves and 108 samples from healthy ewes were available for comparison. Calves: The AT2 consistently overestimated blood glucose concentrations when compared to the PX device with the calves. Correlationt with the PX was r = 0.8496 (canine setting) and r = 0.8861 (feline setting). Both the canine and feline settings demonstrated a consistent bias (41.11 and 33.64 mg/dL, respectively). Ewes: The AT2 consistently overestimated blood glucose concentrations when compared to the PX device with the ewes. Correlation with the PX was R = 0.4710 (canine setting) and R = 0.7269 (feline setting). Both the canine and feline settings demonstrated a consistent bias (21.23 and 14.54 mg/dL, respectively). (4) Clinicians should be aware of the potential for consistent bias when evaluating calf and sheep blood glucose concentrations as the AT2 device, at both settings, overestimated blood glucose compared to the previously validated PX. This reliability appears to change when the values are farther from the normal ranges, which should be considered when making clinical decisions based on data from these devices.
(1) 背景:兽医学中使用多种护理点(POC)血糖仪,但很少进行相互比较。这使得临床医生在比较不同 POC 血糖仪的结果时,有可能无法意识到偏差的影响。(2) 方法:在 "犬科 "和 "猫科 "设置下,同时使用 Precision Xtra 和 AlphaTrak2 这两种 POC 兽用血糖仪对健康小牛和母羊的样本进行比较。通过线性回归和 Bland-Altman 分析对每个样本的结果进行了统计分析。(3) 结果:170 份健康犊牛样本和 108 份健康母羊样本可供比较。犊牛与 PX 设备相比,AT2 始终高估了小牛的血糖浓度。与 PX 的相关性为 r = 0.8496(犬类设置)和 r = 0.8861(猫类设置)。犬科和猫科设置均显示出一致的偏差(分别为 41.11 和 33.64 mg/dL)。母羊:与 PX 设备相比,AT2 始终高估了母羊的血糖浓度。与 PX 的相关性为 R = 0.4710(犬类设置)和 R = 0.7269(猫类设置)。犬科和猫科设置均显示出一致的偏差(分别为 21.23 和 14.54 mg/dL)。(4) 临床医生在评估小牛和绵羊血糖浓度时应注意可能存在一致的偏差,因为与之前验证的 PX 相比,AT2 设备在两种设置下都高估了血糖。当数值偏离正常范围较远时,这种可靠性似乎会发生变化,因此在根据这些设备的数据做出临床决策时应考虑到这一点。
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引用次数: 0
A Novel Direct-Fed Microbial Impacts Growth Performance and Supports Overall Health of Feedlot Cattle 一种新型直接饲喂微生物菌剂可影响饲养场牛的生长性能并促进其整体健康
Pub Date : 2024-06-06 DOI: 10.3390/ruminants4020019
Octavio Guimaraes, Garrett Preedy, J. T. Fox, B. Cappellozza, Ty C. Davis, Jennifer S. Schutz, M. Theurer
Non-hormone-treated beef steers (n = 1625; 371 ± 8.4 kg) were used to determine the impact of a direct-fed microbial (DFM) on growth performance, carcass characteristics, health parameters, and economic outcomes in finishing beef cattle. Steers were blocked based on initial BW, sorted into three optimal marketing groups for each day of enrollment, randomly assigned to one of two treatments (12 replicates per treatment), and fed for an average of 133 days before harvest. Treatments included the following: (1) control (CON) and (2) 50 mg/head per day of a DFM (BDP) containing Lactobacillus animalis 506, Propionibacterium freudenreichii 507, Bacillus licheniformis 809, and Bacillus subtilis 597 (BOVAMINE DEFEND® Plus). Steers were weighed in drafts by pen on a certified scale at closeout, and both pens within a block were harvested on the same day. Carcass characteristics and liver scores were collected upon slaughter. Data were analyzed as part of a completely randomized block design with the pen as the experimental unit. There was no difference in dry matter intake and final body weight between treatments. The BDP steers exhibited a lower number of total outs (deads and removed) throughout the experiment (p < 0.01). Average daily gain (ADG) tended to be greater (p < 0.06), and feed efficiency (p < 0.01) was greater, for steers receiving BDP. Although there were no statistical differences in hot carcass weight and quality grade between treatments, there was a lower incidence of liver abscess scores (p < 0.01) in the BDP steers. These data suggest that BOVAMINE DEFEND® Plus supplementation improves growth performance during the finishing phase, reduces overall mortality, and improves feed to gain efficiency.
使用未经激素处理的肉牛(n = 1625;371 ± 8.4 kg)来确定直接饲喂微生物(DFM)对育成肉牛的生长性能、胴体特征、健康参数和经济效益的影响。根据初始体重对阉牛进行分群,每天将其分为三个最佳销售组,随机分配到两个处理中的一个(每个处理有 12 个重复),并在收获前平均饲喂 133 天。处理包括(1) 对照组(CON);(2) 每天 50 毫克/头的 DFM(BDP),其中含有动物乳杆菌 506、芽孢杆菌 507、地衣芽孢杆菌 809 和枯草芽孢杆菌 597(BOVAMINE DEFEND® Plus)。结账时,用合格的磅秤按栏对阉牛进行称重,同一区块内的两栏阉牛在同一天收获。屠宰时收集胴体特征和肝脏评分。数据分析采用完全随机区组设计,以栏为实验单位。不同处理间的干物质摄入量和最终体重没有差异。在整个实验过程中,BDP 牛的总出栏数(死亡和淘汰)较低(p < 0.01)。接受 BDP 的母牛平均日增重(ADG)往往更高(p < 0.06),饲料效率更高(p < 0.01)。虽然不同处理间的热胴体重量和质量等级没有统计学差异,但 BDP 牛的肝脓肿评分发生率较低(p < 0.01)。这些数据表明,补充 BOVAMINE DEFEND® Plus 可提高育成期的生长性能,降低总死亡率,并提高饲料增重效率。
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引用次数: 0
Estimation of Genetic Parameters for Litter Size and Growth Traits in a Prolific Line of Tunisian Barbarine Sheep 突尼斯滩羊高产品系产仔数和生长性状的遗传参数估计
Pub Date : 2024-06-04 DOI: 10.3390/ruminants4020018
C. Ziadi, Sonia Bedhiaf-Romdhani, Antonio Molina
This study aimed to assess genetic parameters for ewe productivity in a Tunisian Barbarine sheep line. The traits studied were litter size (LS), birth weight (BW), weight at 90 days (W90), and average daily gain between 10 and 30 days (ADG13). A total of 3804 growth and 2726 lambing records were used. Bivariate linear and threshold animal models were fitted and analyzed using the Gibbs sampling methodology. Heritabilities for LS obtained with univariate threshold, bivariate linear, and threshold models were around 0.15, higher than the estimate obtained by a univariate linear model (0.09 ± 0.03). Direct heritability for growth traits remained consistent across models, except for W90 in the bivariate linear threshold model. Maternal heritability for growth traits was higher than direct heritability, ranging from 0.07 to 0.15, except for BW. The covariances between the direct and maternal effects of growth traits were slightly negative. Repeatability oscillated between 0.16 and 0.62. Direct genetic correlations between LS and the other traits were negative, varying from −0.18 (LS-BW) to −0.83 (LS-W90). Our results suggest that the threshold model may be the most appropriate for LS. A selection index including LS and growth traits may be proposed for routine genetic evaluation in this population.
本研究旨在评估突尼斯巴巴里绵羊品系母羊生产性能的遗传参数。研究的性状包括窝产仔数(LS)、出生体重(BW)、90天体重(W90)和10至30天平均日增重(ADG13)。共使用了 3804 份生长记录和 2726 份产羔记录。采用吉布斯抽样方法拟合和分析了二元线性模型和阈值动物模型。单变量阈值、双变量线性和阈值模型得到的LS遗传力约为0.15,高于单变量线性模型得到的估计值(0.09 ± 0.03)。除了双变量线性阈值模型中的 W90 外,各模型中生长性状的直接遗传率保持一致。生长性状的母本遗传率高于直接遗传率,从 0.07 到 0.15 不等,体重除外。生长性状的直接效应和母本效应之间的协方差略呈负值。重复性在 0.16 和 0.62 之间波动。LS 与其他性状之间的直接遗传相关性为负,从-0.18(LS-BW)到-0.83(LS-W90)不等。我们的结果表明,阈值模型可能最适合 LS。建议在该群体的常规遗传评估中使用包括 LS 和生长性状的选择指数。
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引用次数: 0
Sustainable Sheep and Goat Farming in Arid Regions of Jordan 约旦干旱地区的可持续绵羊和山羊养殖
Pub Date : 2024-05-09 DOI: 10.3390/ruminants4020017
Faisal Al-Barakeh, Ashraf Omar Khashroum, Radi Tarawneh, Fatima A. Al-Lataifeh, Azzam N. Al-Yacoub, M. Dayoub, Khaled Al-Najjar
This study examines the sustainability of livestock farming in Jordan’s arid regions, focusing on smallholder Awassi sheep and Baladi goat farming. It assesses breeders, economic factors, herd productivity, and sustainability, evaluating the vitality of these breeds by examining reproductive success through birth and survival rates. In 2021–2022, a total of 53 traditional Awassi sheep and Baladi goat farms in Northern Jordan’s Mafraq Governorate were surveyed using a comprehensive questionnaire to gather data on breeders’ information, farm economics, and herd indicators. Dry seasons in arid lands are tough on sheep and goats, with limited fodder and water availability harming their health and reproductive capacity. While wet seasons bring relief, they can also introduce diseases. Special care for these animals throughout the year is crucial to maintain healthy herds in arid regions. Linear models were employed to analyze sample data, with variance and correlation tests used to explore relationships among variables. Additionally, chi-squared analysis was utilized to assess the impact of male replacement plans on these herds. The main outcomes of this study reveal that breeder indicators pose challenges to the sustainability and growth of Awassi sheep and Baladi goat farming. Conversely, economic indicators suggest a blend of financial stability alongside obstacles related to profitability and resource ownership. Breeder age emerges as a significant factor in livestock herd management, influenced by elements such as experience, resource availability, and adherence to traditional practices. Experience notably influences breeders’ decisions regarding male insemination replacements, likely due to acquired skills. Crop cultivation is shown to reduce fodder costs, thereby enhancing profitability for each young animal sold and consequently increasing the breeders’ returns. Winter conditions elevate animal mortality rates due to cold stress, while other animals within herds contribute to increased kid mortality by facilitating disease transmission. Breeder experience significantly impacts lamb survival, underscoring its importance in ensuring proper care. Increasing ewe proportions within herds enhances lamb production and lowers lamb mortality rates. Effective ram management and yearling lamb sales further contribute to herd health and productivity. A higher proportion of goats within a herd positively correlates with the number of young yearlings sold but inversely affects breeding males and overall kid numbers, indicating a delicate balance that influences herd productivity and mortality rates. Sheep (92.5%) and goat herds (90.6%) demonstrate reproductive success, indicative of superior long-term vitality. While sheep excel in birth rates, survival, and sales, they exhibit lower ewe and lambing percentages. Goat farming demonstrates high birth rates, sales, and overall success but requires particular attention to ensuring kid survival, especially durin
本研究探讨了约旦干旱地区畜牧业的可持续性,重点关注小农阿瓦西绵羊和巴拉迪山羊养殖。研究对饲养者、经济因素、畜群生产力和可持续性进行了评估,通过出生率和存活率考察繁殖成功率,评估了这些品种的生命力。2021-2022 年,我们使用综合问卷调查了约旦北部马弗拉克省的 53 个传统阿瓦西绵羊和巴拉迪山羊养殖场,收集了有关饲养者信息、农场经济和畜群指标的数据。干旱地区的旱季对绵羊和山羊来说十分难熬,有限的饲料和水源损害了它们的健康和繁殖能力。雨季虽然能缓解旱情,但也会带来疾病。全年对绵羊和山羊的特殊照顾对于在干旱地区维持健康的羊群至关重要。采用线性模型分析样本数据,并通过方差和相关性检验来探讨变量之间的关系。此外,还利用卡方分析评估了雄性替代计划对这些牛群的影响。这项研究的主要结果表明,种羊指标对阿瓦西绵羊和巴拉迪山羊养殖业的可持续性和增长构成了挑战。与此相反,经济指标表明,财务稳定性与盈利能力和资源所有权方面的障碍并存。饲养员的年龄是畜群管理的一个重要因素,受经验、资源可用性和传统习俗等因素的影响。经验明显影响着饲养者对雄性人工授精替代品的决定,这可能是由于掌握了技能。农作物种植可降低饲料成本,从而提高每头幼畜的销售利润,进而增加饲养者的收益。冬季条件下,动物死亡率会因寒冷压力而升高,而牛群中的其他动物会促进疾病传播,从而导致羔羊死亡率升高。饲养员的经验对羔羊的存活率有很大影响,这突出了其在确保适当照料方面的重要性。提高羊群中母羊的比例可提高羔羊产量并降低羔羊死亡率。有效的公羊管理和一岁羔羊销售可进一步促进羊群健康和生产率。羊群中较高的山羊比例与一岁羔羊的销售数量呈正相关,但与种公羊和羔羊总数成反比,这表明影响羊群生产率和死亡率的微妙平衡。绵羊群(92.5%)和山羊群(90.6%)都取得了繁殖成功,这表明它们具有卓越的长期生命力。虽然绵羊在出生率、存活率和销售方面表现出色,但母羊和羔羊的比例较低。山羊养殖表现出较高的出生率、销售量和整体成功率,但需要特别注意确保羔羊的存活率,尤其是在冬季。改善阿瓦西绵羊群和巴拉迪山羊群的特性对于确保可持续发展至关重要,可以通过在干旱地区采取适当的护理措施来实现。总之,尽管存在挑战,但约旦的绵羊和山羊养殖业仍有很大的发展前景。借鉴饲养者的经验和经济学知识进行有效管理至关重要。增强小规模牧民的能力,实施有针对性的干预措施,如冬季死亡率解决方案和选择性育种,对于可持续发展至关重要。
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引用次数: 0
Stressors Inherent to Beef Cattle Management in the United States of America and the Resulting Impacts on Production Sustainability: A Review 美国肉牛管理的固有压力及其对生产可持续性的影响:综述
Pub Date : 2024-05-08 DOI: 10.3390/ruminants4020016
Toriann Summer Winton, Molly Christine Nicodemus, K. Harvey
Stressors are directly related to major events throughout the beef cattle production cycle. Understanding the impact stressors have on productive outcomes is critical for the efficient implementation of management strategies. Such stressors include environmental extremes, nutritional deprivation, and common management procedures. Environmental extremes such as thermal stress can disturb gestating cows’ normal physiological responses, hindering reproductive efficiency. Thermal stress during the breeding season can affect embryo development causing a decrease in conception rates, although adjusting the scheduling of breeding activities can minimize losses. Additionally, suboptimal nutrition may negatively impact reproductive performance if management strategies including modifying seasonal grazing practices are not implemented. As gestation progresses, nutrient requirements increase; thus, without appropriate dietary management, poor calf performance, the loss of the body condition score, and reduced reproductive performance may result. While weaning is a common management procedure, this event is another major stress within the production system. Applying efficient strategies such as creep feeding or two-step weaning to mitigate weaning stress can maximize production efficiency. This review will explore in-depth the stressors associated with production events in the beef cattle industry and give insight into researched management strategies targeting these stressors that will improve the sustainability of the production system.
应激因素与肉牛生产周期中的重大事件直接相关。了解应激因素对生产结果的影响对于有效实施管理策略至关重要。这些应激因素包括极端环境、营养匮乏和常见的管理程序。极端环境(如热应激)会干扰妊娠母牛的正常生理反应,阻碍繁殖效率。配种季节的热应激会影响胚胎发育,导致受孕率下降,不过调整配种活动的时间安排可将损失降至最低。此外,如果不实施包括改变季节性放牧方式在内的管理策略,营养不足也会对繁殖性能产生负面影响。随着妊娠的进行,营养需求会增加;因此,如果没有适当的饮食管理,可能会导致犊牛表现不佳、体况评分下降和繁殖性能降低。虽然断奶是一个常见的管理程序,但这是生产系统中的另一个主要压力。采用爬行饲喂或两步断奶等有效策略来减轻断奶压力,可最大限度地提高生产效率。本综述将深入探讨肉牛业中与生产事件相关的应激因素,并深入分析针对这些应激因素研究出的管理策略,以提高生产系统的可持续性。
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引用次数: 0
Drinking Behaviour of Beef Cattle Subject to Water Medication in Various Environmental Conditions 不同环境条件下接受饮水药物治疗的肉牛的饮水行为
Pub Date : 2024-04-17 DOI: 10.3390/ruminants4020015
E. P. Romanzini, Vivienne McCollum, Sarah Mcilveen, Kawane Dias da Silva, William Luiz de Souza, Priscila Arrigucci Bernardes, Diogo Fleury Azevedo Costa
Two experiments were conducted to assess the effects of water medication technology on beef cattle behaviour and performance in tropical conditions. Experiment 1 involved 30 Droughtmaster yearling steers monitored over seven days in a controlled environment. Feed and water consumptions were monitored with Smart Feed Pro® systems, with three water treatments administered via uDOSE® technology. The results indicated an average water intake of 13.6 L/head/d. Experiment 2 had 120 yearling steers from four genetic groups grazing on an extensive pasture system. Throughout four 24-day periods, forage availability and chemical composition were measured once monthly. Experiment 2 revealed a variation in water intake, ranging from 16.2 L/head/d down to 4.75 L/head/d. Notably, the lower intake coincided with a rainfall event documented during the fourth experimental period. Overall, results from both experiments indicated that water medication did not alter cattle water preference. There was no preference for treated water sources in Experiment 1, while differences in Experiment 2 appeared to be influenced by external factors like weather and prior habits. These experiments demonstrate the feasibility of water medication for beef cattle without disruption of their natural behaviour.
我们进行了两项实验,以评估在热带条件下水药技术对肉牛行为和性能的影响。实验 1 涉及 30 头 Droughtmaster 一岁牛,在受控环境中进行了为期七天的监测。使用 Smart Feed Pro® 系统监控饲料和水的消耗量,并通过 uDOSE® 技术进行三种水处理。结果表明,平均饮水量为 13.6 升/头/天。实验 2 让来自四个基因组的 120 头一岁犊牛在大面积牧草系统上放牧。在四个为期 24 天的时间里,每月测量一次牧草的可用性和化学成分。实验 2 显示,采食量从 16.2 升/头/天到 4.75 升/头/天不等。值得注意的是,较低的摄水量与第四个实验期的降雨事件相吻合。总之,两次实验的结果都表明,饮水药物不会改变牛的饮水偏好。在实验 1 中,牛只对处理过的水源没有偏好,而在实验 2 中,牛只对水源的偏好似乎受到天气和先前习惯等外部因素的影响。这些实验证明了在不破坏肉牛自然行为的情况下对其进行饮水药物治疗的可行性。
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引用次数: 0
Hot Iron Branding of Beef Cattle: Process Characterization, Implications for Animal Welfare, and Its Efficiency for Cattle Individual Identification 肉牛的热铁烙印:工艺特征、对动物福利的影响及其对牛个体识别的效率
Pub Date : 2024-04-09 DOI: 10.3390/ruminants4020013
Jaira de Oliveira, Joseph Grajales-Cedeño, Mariana Parra Cerezo, T. Valente, M. P. D. Paranhos da Costa
This study aimed to characterize the hot iron branding (HIB) procedure by assessing its implications for animal welfare and its efficiency for cattle identification. The study was carried out in two stages: First, with 37 Nellore calves, by measuring the skin temperatures in the place of HIB application (ONB) and 10 cm above it (OFFB) immediately after its application and during four consecutive days, the time required for application of each HIB digit and the occurrences of rebranding; second, with two batches of cows (N = 97 and N = 94, respectively, by measuring the time spent to read cattle ID and comparing the efficiency of HIB vs. EET (electronic ear tag) and visual ear tags (VET) vs. EET. Skin temperature was significantly affected by the interaction between the place where the skin temperatures were taken (on and 10 cm above the HIB) and assessment day, with temperatures in ONB on days d0 and d2 being higher than in OFFB (p < 0.05), and 86% of the calves required at least one rebranding. EET reading was faster than HIB and VET (p < 0.001), and fewer errors were made when reading EET than HIB (1/97 vs. 17/97) and VET (2/94 vs. 12/94). We concluded that HIB potentially compromises cattle welfare and has a lower efficiency for cattle identification than EET and VET.
本研究旨在通过评估热烙铁(HIB)烙印程序对动物福利的影响及其识别牛只的效率,确定热烙铁烙印程序的特点。研究分两个阶段进行:首先,研究对象是 37 头内洛尔犊牛,方法是测量烙铁熨烫处(ONB)和烙铁熨烫处上方 10 厘米处(OFFB)在烙铁熨烫后立即和连续四天内的皮肤温度、烙铁熨烫每个数字所需的时间以及重新烙印的发生率;其次,研究对象是两批奶牛(分别为 97 头和 94 头),方法是测量读取牛标识所需的时间,并比较烙铁熨烫与电子耳标(EET)和可视耳标(VET)与电子耳标的效率。采集皮温的地点(HIB上和HIB上方10厘米处)与评估日之间的交互作用对皮温有明显影响,第0天和第2天ONB的皮温高于OFFB(p < 0.05),86%的犊牛至少需要重新标记一次。EET 读数比 HIB 和 VET 更快(p < 0.001),EET 读数的错误率比 HIB(1/97 对 17/97)和 VET(2/94 对 12/94)更低。我们的结论是,与 EET 和 VET 相比,HIB 可能会损害牛的福利,而且识别牛的效率较低。
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引用次数: 0
Combining Embryo Transfer and Artificial Insemination to Achieve Twinning in Beef Cattle, and Effects of Different Twin Calf-Raising Methods on Neonatal Behavior and Growth 结合胚胎移植和人工授精实现肉牛双胞胎,以及不同双胞胎犊牛饲养方法对新生儿行为和生长的影响
Pub Date : 2024-04-09 DOI: 10.3390/ruminants4020014
E. M. Bortoluzzi, K. Aubuchon, Nicole D. Robben, Nicole Stafford, M. J. Goering, Claiborn Bronkhorst, J. A. Odde, Clay Breiner, Karol Fike, Lindsey E. Hulbert, Kenneth G. Odde
As the beef industry moves towards efficient animal production to improve sustainability in agriculture, new production and management approaches are emerging. Among the many facets of the beef industry, cow–calf operations have the most opportunity for efficiency improvement, including improvements in fertility. This project accounts for measures and methods of (1) twinning reproductive technologies and (2) twin calf perinatal care and pre-weaning rearing. The overall objective was to produce twin calves using two reproductive technologies—embryo transfer and artificial insemination. The subobjectives were to determine accuracy of twin pregnancies embryo/fetal losses using ultrasonography, evaluate parturition and dystocia, and determine the effects of different twin-raising methods on neonatal behavior and growth. A fixed-time artificial insemination (FTAI) protocol was applied to 77 multiparous Angus-cross cows from a commercial beef herd in northcentral South Dakota during the summer of 2019. Cows were assigned to two different treatments groups: only artificially inseminated (AI) or received an embryo transfer following artificial insemination (ET + AI). They were estrous-synchronized, artificially inseminated (AI) with black Angus semen at day 0, and received and embryo transfer (ET) at day 7. Ultrasound examination detected 56% pregnancy risk for both groups, with sensitivity, specificity, and accuracy of 75%, 100%, and 90.5%, respectively, for bilateral twin detection. Calves were born during spring 2020. Twin calves (n = 34) and singleton calves (n = 11) were assigned to one of three raising methods: (1) twin born and twin raised (TT; n = 16), (2) twin born and single raised (TS; n = 18), and (3) single born and single raised (S; n = 11). Neonatal nursing behavior and birth weights were recorded, and adjusted day 200 and day 280 were calculated measures of vitality and growth. Blood samples were collected at age 24 h for colostrum intake measures (total serum protein, IgG1, and IgM). Twin calves were born 20% (p < 0.05) lighter in body weight than singletons; however, weights did not differ at day 280 between TT and S calves. TS calves had the shortest average latency to stand, but immunoglobulin concentrations did not differ among treatments. At weaning, cows that had birthed and raised twins produced more kilograms of live weight per pregnancy than cows birthing and raising singletons. Using ET + AI proved to increase twinning rate, and growth was maintained when raising both twins with their dam.
随着牛肉业朝着高效畜牧生产的方向发展,以提高农业的可持续性,新的生产和管理方法不断涌现。在牛肉产业的诸多方面中,牛犊饲养最有机会提高效率,包括提高繁殖力。该项目包括以下措施和方法:(1) 双胎繁殖技术;(2) 双胎犊牛围产期护理和断奶前饲养。总体目标是利用胚胎移植和人工授精这两种繁殖技术生产双胞胎犊牛。子目标是利用超声波检查确定双胎妊娠胚胎/胎儿损失的准确性,评估分娩和难产情况,并确定不同的双胎饲养方法对新生儿行为和生长的影响。2019年夏季,南达科他州中北部一个商业化肉牛群的77头多胎安格斯杂交奶牛采用了固定时间人工授精(FTAI)方案。奶牛被分配到两个不同的处理组:只进行人工授精(AI)或在人工授精后进行胚胎移植(ET + AI)。它们进行发情同步,在第0天使用黑安格斯精液进行人工授精(AI),并在第7天接受胚胎移植(ET)。超声波检查发现两组妊娠风险均为 56%,双侧双胞胎检测的灵敏度、特异性和准确性分别为 75%、100% 和 90.5%。小牛于 2020 年春季出生。双胞胎犊牛(n = 34)和单胎犊牛(n = 11)被分配到三种饲养方法中的一种:(1) 双生双养(TT;n = 16);(2) 双生单养(TS;n = 18);(3) 单生单养(S;n = 11)。记录新生儿的哺乳行为和出生体重,并计算调整后的第 200 天和第 280 天的活力和生长情况。在犊牛出生后 24 小时采集血液样本,用于测量初乳摄入量(血清总蛋白、IgG1 和 IgM)。双胞胎犊牛出生时的体重比单胎犊牛轻 20% (p < 0.05);但是,TT 和 S 型犊牛在出生后第 280 天的体重没有差异。TS犊牛的平均站立潜伏期最短,但不同处理间的免疫球蛋白浓度并无差异。断奶时,分娩和哺育双胞胎的奶牛比分娩和哺育单胎的奶牛每胎生产的活重更多。事实证明,使用ET+人工授精可提高双胞胎率,而且与母牛一起饲养双胞胎时,可保持生长。
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