Pub Date : 2024-01-16DOI: 10.9734/sajrm/2024/v18i1342
Christiana N. Opara, F. I. Omeje
This study aims to screen fungal isolates from sun dried meat samples to identify fungal presumed producing mycotoxins in the sun dried meat. A total of 8 fungal isolates from sun dried meat were used for the study. They are Aspergillus (2), Penicillium spp (3), Fusarium spp, Mucor spp and Rhizopus spp. The fungal isolates from eight sun dried-meat samples were sub-cultured on Potato Dextrose Agar and Sabouraud Dextrose agar in order to obtain pure colonies for further analysis. The detection of mycotoxins in the sun dry meat samples was carried out by the use of Coconut Agar Media (CAM), by which the fungal isolates were cultured and plates incubated for 3-7days at 28C. The results obtained show that some of the fungal isolates had the ability to produce mycotoxins. From the mycotoxin screening, only four (4) species (Aspergillus spps, and Penicillium spp) were positive (+) and Rhizopus spp, Fusarium spp and Mucor spp, were negative (-). Mycotoxin contamination level varied in different market locations in which Academy Market had the highest level of mycotoxin (71.4%) followed by Oje Market (14.3%) and Oremiji Market (14.3%). Mycotoxigenic producing fungi and mycotoxin levels in the sun dried meat samples are public health concern. Also these results calls for more sensitization for safety of sun dried meat.
{"title":"Primary Screening for Fungi Isolates that Produce Mycotoxin from Sun-Dried Meat","authors":"Christiana N. Opara, F. I. Omeje","doi":"10.9734/sajrm/2024/v18i1342","DOIUrl":"https://doi.org/10.9734/sajrm/2024/v18i1342","url":null,"abstract":"This study aims to screen fungal isolates from sun dried meat samples to identify fungal presumed producing mycotoxins in the sun dried meat. A total of 8 fungal isolates from sun dried meat were used for the study. They are Aspergillus (2), Penicillium spp (3), Fusarium spp, Mucor spp and Rhizopus spp. The fungal isolates from eight sun dried-meat samples were sub-cultured on Potato Dextrose Agar and Sabouraud Dextrose agar in order to obtain pure colonies for further analysis. The detection of mycotoxins in the sun dry meat samples was carried out by the use of Coconut Agar Media (CAM), by which the fungal isolates were cultured and plates incubated for 3-7days at 28C. The results obtained show that some of the fungal isolates had the ability to produce mycotoxins. From the mycotoxin screening, only four (4) species (Aspergillus spps, and Penicillium spp) were positive (+) and Rhizopus spp, Fusarium spp and Mucor spp, were negative (-). Mycotoxin contamination level varied in different market locations in which Academy Market had the highest level of mycotoxin (71.4%) followed by Oje Market (14.3%) and Oremiji Market (14.3%). Mycotoxigenic producing fungi and mycotoxin levels in the sun dried meat samples are public health concern. Also these results calls for more sensitization for safety of sun dried meat.","PeriodicalId":509356,"journal":{"name":"South Asian Journal of Research in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139620384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-12DOI: 10.9734/sajrm/2024/v18i1340
Abdullahi K., Innocent W. J., Hafsat S. B., Habibu M., Yusuf Abdurrahman
The study was conducted to isolate and identify the bacteria, to know the sources of contamination of milk and antibiotic sensitivity of bacteria obtained from Dutsin-Ma, Katsina State. A total of 45 samples were collected from different locations in Dutsin-Ma such as Wednesday market, opposite the FUDMA takeoff site and Hospital road. All these samples were analyzed by culturing in different media such as Salmonella-Shigella agar, Eosin Methylene Blue agar, Mannitol Salt Agar, Nutrient agar, Cetrimide agar, and MacConkey agar. Biochemical tests were performed to identify the organism. Among 45 samples, 20 (37.7%) were Staphylococcus spp. Similarly, 11 (20.8%), 6 (11.3%), 4(7.5%) and 12(22.6%) were found positive for Escherichia coli, Pseudomonas aeruginosa, Klebsiella and Salmonella spp. and. respectively. Results of the antibiotic sensitivity test represent that, out of ten antibiotics Staphylococcus sp. were very sensitive against Gentamicin (95%), Ciprofloxacin (90%), Streptomycin (70%), and highly resistant against Zinnacef (60%), Ampiclox (70%), Amoxicillin (50%). Salmonella sp. were highly sensitive to Pefloxacin (83.3%), Sparfloxacin (83.3%), and Ciprofloxacin (95%), but resistant against Augmentin (83.3%), Streptomycin (75%), Sulfamethoxazole (66.6%). Klebsiella spp. were highly sensitive to Pefloxacin (50%), Sparfloxacin, Chloramphenicol (75%), and Ciprofloxacin (75%), but resistant to Gentamicin (100%), Streptomycin (100%), Sulfamethoxazole (75%), Augmentin (75%). Escherichia coli were highly sensitive to Gentamicin (72.7%), Ciprofloxacin (100%), Ofloxacin (90.9%), Sparfloxacin (72.7%), but highly resistant to Sulphamethoxazole (72.2%), Whereas, Pseudomonas aeruginosa were highly sensitive Ciprofloxacin (100%), Chloramphenicol (66.6%), Ofloxacin (66.6%), but highly resistant against Streptomycin (100%), Augmentin 83.35%), Perfloxacin (83.3%). Data from this study suggested that raw milk contaminated with drug-resistant bacteria may cause public health hazards.
{"title":"Isolation, Identification and Antibiogram of Bacteria Isolated from Raw Cow Milk in Dutsin-Ma, Katsina State","authors":"Abdullahi K., Innocent W. J., Hafsat S. B., Habibu M., Yusuf Abdurrahman","doi":"10.9734/sajrm/2024/v18i1340","DOIUrl":"https://doi.org/10.9734/sajrm/2024/v18i1340","url":null,"abstract":"The study was conducted to isolate and identify the bacteria, to know the sources of contamination of milk and antibiotic sensitivity of bacteria obtained from Dutsin-Ma, Katsina State. A total of 45 samples were collected from different locations in Dutsin-Ma such as Wednesday market, opposite the FUDMA takeoff site and Hospital road. All these samples were analyzed by culturing in different media such as Salmonella-Shigella agar, Eosin Methylene Blue agar, Mannitol Salt Agar, Nutrient agar, Cetrimide agar, and MacConkey agar. Biochemical tests were performed to identify the organism. Among 45 samples, 20 (37.7%) were Staphylococcus spp. Similarly, 11 (20.8%), 6 (11.3%), 4(7.5%) and 12(22.6%) were found positive for Escherichia coli, Pseudomonas aeruginosa, Klebsiella and Salmonella spp. and. respectively. Results of the antibiotic sensitivity test represent that, out of ten antibiotics Staphylococcus sp. were very sensitive against Gentamicin (95%), Ciprofloxacin (90%), Streptomycin (70%), and highly resistant against Zinnacef (60%), Ampiclox (70%), Amoxicillin (50%). Salmonella sp. were highly sensitive to Pefloxacin (83.3%), Sparfloxacin (83.3%), and Ciprofloxacin (95%), but resistant against Augmentin (83.3%), Streptomycin (75%), Sulfamethoxazole (66.6%). Klebsiella spp. were highly sensitive to Pefloxacin (50%), Sparfloxacin, Chloramphenicol (75%), and Ciprofloxacin (75%), but resistant to Gentamicin (100%), Streptomycin (100%), Sulfamethoxazole (75%), Augmentin (75%). Escherichia coli were highly sensitive to Gentamicin (72.7%), Ciprofloxacin (100%), Ofloxacin (90.9%), Sparfloxacin (72.7%), but highly resistant to Sulphamethoxazole (72.2%), Whereas, Pseudomonas aeruginosa were highly sensitive Ciprofloxacin (100%), Chloramphenicol (66.6%), Ofloxacin (66.6%), but highly resistant against Streptomycin (100%), Augmentin 83.35%), Perfloxacin (83.3%). Data from this study suggested that raw milk contaminated with drug-resistant bacteria may cause public health hazards.","PeriodicalId":509356,"journal":{"name":"South Asian Journal of Research in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139624200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-05DOI: 10.9734/sajrm/2024/v18i1338
Adeka P., Imandeh G. N., Ikpa T. F., Okafor, I. D.
Background: Malaria remains a persistent public health challenge, particularly in sub-Saharan Africa, where asymptomatic falciparum malaria poses a significant threat. Asymptomatic cases serve as a crucial parasite reservoir, contributing to ongoing transmission. Aim: The study investigates the prevalence of asymptomatic falciparum malaria and assesses the occurrence of gene deletion in the Plasmodium falciparum Histidine Rich Protein 2 (Pf HRP2) gene in Makurdi, Benue State, Nigeria. Methodology: A cross-sectional study was conducted between September and October 2019, which involved 374 apparently healthy individuals from five communities. Malaria diagnosis utilized Rapid Diagnostic Test (RDT) kits, microscopy, and polymerase chain reaction (PCR) for Pf HRP2 gene assessment. Results: The study found a prevalence of 25.4% by RDT and 28.1% by microscopy. Asymptomatic falciparum malaria was significantly influenced by location, proximity to water bodies, bed net usage, and history of malaria treatment, while, age, and insecticide usage showed no significant impact. PCR results revealed amplified fragments with band sizes ranging from 600 to 900 base pairs in 40 positive isolates, eliminating Pf HRP2 gene deletion as a cause for false negatives observed between RDT and microscopy results. Discussion/Conclusion: The study highlights a high malaria transmission rate in Nigeria, emphasizing the role of location-specific factors and bed net usage in the proliferation of asymptomatic falciparum malaria. Importantly, no gene deletion was identified in the Pf HRP2 gene among the studied Plasmodium falciparum isolates.
{"title":"Prevalence of Asymptomatic Falciparum Malaria, Risk Factors, and Absence of PfHRP2 Gene Deletion in Makurdi, Nigeria","authors":"Adeka P., Imandeh G. N., Ikpa T. F., Okafor, I. D.","doi":"10.9734/sajrm/2024/v18i1338","DOIUrl":"https://doi.org/10.9734/sajrm/2024/v18i1338","url":null,"abstract":"Background: Malaria remains a persistent public health challenge, particularly in sub-Saharan Africa, where asymptomatic falciparum malaria poses a significant threat. Asymptomatic cases serve as a crucial parasite reservoir, contributing to ongoing transmission. \u0000Aim: The study investigates the prevalence of asymptomatic falciparum malaria and assesses the occurrence of gene deletion in the Plasmodium falciparum Histidine Rich Protein 2 (Pf HRP2) gene in Makurdi, Benue State, Nigeria. \u0000Methodology: A cross-sectional study was conducted between September and October 2019, which involved 374 apparently healthy individuals from five communities. Malaria diagnosis utilized Rapid Diagnostic Test (RDT) kits, microscopy, and polymerase chain reaction (PCR) for Pf HRP2 gene assessment. \u0000Results: The study found a prevalence of 25.4% by RDT and 28.1% by microscopy. Asymptomatic falciparum malaria was significantly influenced by location, proximity to water bodies, bed net usage, and history of malaria treatment, while, age, and insecticide usage showed no significant impact. PCR results revealed amplified fragments with band sizes ranging from 600 to 900 base pairs in 40 positive isolates, eliminating Pf HRP2 gene deletion as a cause for false negatives observed between RDT and microscopy results. \u0000Discussion/Conclusion: The study highlights a high malaria transmission rate in Nigeria, emphasizing the role of location-specific factors and bed net usage in the proliferation of asymptomatic falciparum malaria. Importantly, no gene deletion was identified in the Pf HRP2 gene among the studied Plasmodium falciparum isolates.","PeriodicalId":509356,"journal":{"name":"South Asian Journal of Research in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139450410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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