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Characterization of the physicochemical, bromatological properties, and antioxidant activity of powdered sugarcane bagasse 甘蔗渣粉的物理化学、溴化特性和抗氧化活性的表征
Pub Date : 2024-04-19 DOI: 10.47280/revfacagron(luz).v41.n2.04
Alanís Cabrera, Maritza Velásquez, osé Muñoz
By-products are currently considered important foods for human consumption due to their large contribution of bioactive compounds. The objective of the study was to characterize the physicochemical, bromatological, and antioxidant properties of powdered sugarcane bagasse. To obtain sugarcane bagasse powder (PBCA), samples were collected in three artisanal sugar mills in the Junín canton, province of Manabí. The samples were labeled under the codes; M1, M2, and M3. An analysis of variance and Tukey's test at 5 % significance were applied. Statistical significance was determined between the samples evaluated, the results demonstrated a variation in the physicochemical properties: pH (5.96 ± 0.01 – 7.14 ± 0.05); acidity (0.09 ± 0.00 – 0.37 ± 0.00 %); moisture (5.05 ± 0.32 – 9.80 ± 0.68 %) and ash (1.94 ± 0.00 – 4.47 ± 0.02 %), bromatological: crude fiber (13.85 ± 0.11 – 24.39%); protein (0.16 ± 0.00 – 0.86 ± 0.01 %); dry matter (88.52 ± 3.51 – 94.94 ± 0.32%) and fat (0.09 ± 0.00 – 0.13 ± 0.01%), functional and antioxidant compounds: hemicellulose (25.32 ± 0.79 %); cellulose (17.90 ± 0.05 – 26.83 ± 0.20%); lignin (0.31 ± 0.00 – 0.51 ± 0.00 %); water retention capacity (3.27 ± 0.01 – 4.93 ± 0.19 g H2O.g-1); antioxidant activity (3.70 ± 0.03 – 9.92 ± 9.12 µmol trolox equivalent.g-1) and total phenols (2.19 ± 0.00 – 13.35 ± 0.03 mg gallic acid equivalent.g-1). All samples were microbiologically acceptable. PBCA presented nutritional characteristics of importance for the formulation of products for human consumption.
副产品由于含有大量生物活性化合物,目前被认为是人类食用的重要食品。这项研究的目的是鉴定甘蔗渣粉的物理化学、溴化物和抗氧化特性。为了获得甘蔗渣粉(PBCA),研究人员在马纳比省胡宁县的三家手工制糖厂采集了样品。样本分别以 M1、M2 和 M3 编码标示。采用方差分析和 Tukey's 5 % 显著性检验。结果显示理化特性存在差异:pH 值(5.96 ± 0.01 - 7.14 ± 0.05);酸度(0.09 ± 0.00 - 0.37 ± 0.00 %);水分(5.05 ± 0.32 - 9.80 ± 0.68 %)和灰分(1.94 ± 0.00 - 4.47 ± 0.02 %);溴化物:粗纤维(13.85 ± 0.11 - 24.39 %);蛋白质(0.16 ± 0.00 - 0.86 ± 0.01 %);干物质(88.52 ± 3.51 - 94.94 ± 0.32 %)和脂肪(0.09 ± 0.00 - 0.13 ± 0.01 %),功能性和抗氧化化合物:半纤维素(25.32 ± 0.79 %);纤维素(17.90 ± 0.05 - 26.83 ± 0.20 %);木质素(0.31 ± 0.00 - 0.51 ± 0.00 %);保水能力(3.27 ± 0.01 - 4.93 ± 0.19 g H2O.g-1);抗氧化活性(3.70 ± 0.03 - 9.92 ± 9.12 µmol trolox equivalent.g-1)和总酚(2.19 ± 0.00 - 13.35 ± 0.03 mg 没食子酸当量.g-1)。所有样品在微生物方面都是合格的。PBCA 具有重要的营养特性,可用于配制供人类食用的产品。
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引用次数: 0
Physical and sensory quality of coffee dried in three prototypes of greenhouse solar dryers 在三种原型温室太阳能干燥器中干燥的咖啡的物理和感官质量
Pub Date : 2024-04-09 DOI: 10.47280/revfacagron(luz).v41.n2.02
María Huamán-Murillo, Shirley Mejía, Silvia Murillo-Baca, Fortunato Ponce-Rosas, Maria Fuentes-Meza
Reducing the drying time of coffee without causing negative effects on cup quality has been the subject of several investigations. The objective was to determine the effect of three variants of a greenhouse-type solar dryer prototype on the physical, sensory and microbiological quality of coffee. The solar dryer prototype with three variants, prototype 1 (P1) greenhouse solar dryer, prototype 2 (P2) solar dryer with air extractor and prototype 3 (P3) solar dryer with air extractor and solar powered heaters, was compared to traditional drying (T0) (cement floor). Drying time, physical grain defects, yield, sensory and microbiological quality were evaluated. The best results were obtained in P3 with a drying time of 52 h (2 ¼ days), with dry parchment coffee beans without primary defects, higher yield in exportable gold coffee (83.24 %), sensory profile of 84.25 points qualified as specialty coffee (with Q Premium grade) and better safety than coffee obtained by traditional drying; demonstrating that the greenhouse type solar dryer with air extractor and solar energy heaters is a sustainable alternative to improve the efficiency of coffee drying while preserving its sensory quality, an essential value for producers to achieve better prices.
在不对咖啡杯质量造成负面影响的情况下缩短咖啡干燥时间一直是多项研究的主题。这项研究的目的是确定温室型太阳能干燥器原型的三种变体对咖啡物理、感官和微生物质量的影响。该太阳能烘干机原型有三种变体,分别是原型 1(P1)温室太阳能烘干机、原型 2(P2)带空气提取器的太阳能烘干机和原型 3(P3)带空气提取器和太阳能加热器的太阳能烘干机,并与传统烘干(T0)(水泥地面)进行了比较。对干燥时间、谷物物理缺陷、产量、感官和微生物质量进行了评估。P3 获得了最佳结果,干燥时间为 52 小时(2 ¼ 天),干燥的羊皮纸咖啡豆没有初级缺陷,出口金咖啡产量更高(83.24%),感官质量 84.25 分,符合特种咖啡(Q 级特等)标准,安全性优于传统干燥方式获得的咖啡;这表明,带有空气抽吸器和太阳能加热器的温室型太阳能干燥器是一种可持续的替代方法,既能提高咖啡干燥效率,又能保持其感官质量,这是生产商获得更高价位的重要价值。
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引用次数: 0
Molecular variation of lipoxygenase-associated genes in grain of commercial Mexican soybean cultivars 墨西哥商品大豆品种谷粒中脂氧合酶相关基因的分子变异
Pub Date : 2024-03-17 DOI: 10.47280/revfacagron(luz).v41.n2.01
Mónica López, Ana Sifuentes, Francisco Paredes, Nicolás Maldonado, Homar Gill
Lipoxygenase enzymes encoded by the Lox1, Lox2 and Lox3 genes play a crucial role in soybean grain, particularly in the development of off-flavors. Understanding molecular variation within Lox genes is essential for the improvement of soybean organoleptic traits. This study investigated the genetic variation in the internal regions of the Lox1, Lox2, and Lox3 genes in mature grain of commercially grown soybean cultivars in Mexico. Genomic DNA from a diverse panel of Mexican soybean cultivars was analyzed using resequencing techniques and in-silico analysis. Single nucleotide polymorphisms (SNP) within the Lox1, Lox2, and Lox3 genes were identified and characterized. The findings indicated that Lox3 gene displayed lower genetic variability compared to Lox1 and Lox2 genes, specifically, was identified a total of 26 SNPs in the Lox1 gene, 11 SNPs in the Lox2 gene, and 5 SNPs in the Lox3 gene among the examined cultivars. A non-synonymous SNP variant of the C/C genotype located in exon 6 of the Lox2 gene was associated with a destabilizing effect on the lipoxygenase 2 enzyme in the Guayparime S-10 and Huasteca 300 cultivars. These findings provide insights into the molecular variation of lipoxygenase-associated genes in Mexican soybean cultivars.
由 Lox1、Lox2 和 Lox3 基因编码的脂氧合酶在大豆谷物中发挥着至关重要的作用,尤其是在异味的形成过程中。了解 Lox 基因内部的分子变异对改良大豆感官性状至关重要。本研究调查了墨西哥商业种植的大豆栽培品种成熟谷粒中 Lox1、Lox2 和 Lox3 基因内部区域的遗传变异。研究人员采用重测序技术和内部分析方法,对墨西哥大豆栽培品种的基因组 DNA 进行了分析。对 Lox1、Lox2 和 Lox3 基因中的单核苷酸多态性(SNP)进行了鉴定和表征。研究结果表明,与 Lox1 和 Lox2 基因相比,Lox3 基因的遗传变异性较低,具体而言,在所研究的栽培品种中,Lox1 基因中共鉴定出 26 个 SNP,Lox2 基因中共鉴定出 11 个 SNP,Lox3 基因中共鉴定出 5 个 SNP。在 Guayparime S-10 和 Huasteca 300 栽培品种中,位于 Lox2 基因第 6 外显子的 C/C 基因型非同义 SNP 变异与脂氧合酶 2 酶的不稳定性有关。这些发现有助于深入了解墨西哥大豆栽培品种中脂氧合酶相关基因的分子变异。
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引用次数: 0
Effect of grazing on the composition and floristic diversity during fallow at El-Tarf, Algeria 放牧对阿尔及利亚 El-Tarf 地区休耕期间植物组成和植物多样性的影响
Pub Date : 2024-03-13 DOI: 10.47280/revfacagron(luz).v41.n1.10
Saida Matallah, Fouzi Matallah
In the El-Tarf region, located in northeastern Algeria, fallow lands are frequently used as grazing grounds for sheep flocks, where they are left all day with a significant grazing load. Limited studies conducted to assess the effects of grazing have yielded varied and sometimes contradictory results, thus underscoring the need for reliable local data. The study aims to assess the impact of grazing on the floristic richness, diversity, production, and chemical composition of fallow lands in the El-Tarf region (northeast Algeria). Measurements were taken during the optimal vegetation period from March to June over a period of 2 years (2019 and 2020). The experimental design included a single factor: grazing intensity (no grazing, moderate grazing, and intense grazing). The results indicated that grazing reduced floristic richness and diversity (Maximum Diversity, Shannon and Weaver Index, Evenness Index), but it did not have an effect on coverage. The non-grazed fallow land is richer, hosting 23 herbaceous species distributed among 8 botanical families, with the most represented being Asteraceae (39 %), followed by Poaceae (26 %). The Fabaceae, on the other hand, display a more modest representation, accounting for only 13 %.Grazing also decreased significantly the biomass and total aboveground herbaceous phytomass, with improvements noted in non-grazed conditions, recording values of 3.80 t.DM.ha-1 and 1.77 t.DM.ha-1, respectively. In addition, grazing had a significant impact on chemical composition, with the highest values recorded for moderate grazing, reaching 14.7 % for total nitrogen content and 11.66 % for mineral content.
在位于阿尔及利亚东北部的 El-Tarf 地区,休耕地经常被用作羊群的牧场,羊群整天都在那里吃草。为评估放牧的影响而进行的有限研究得出的结果各不相同,有时甚至相互矛盾,这突出表明需要可靠的当地数据。本研究旨在评估放牧对 El-Tarf 地区(阿尔及利亚东北部)休耕地的植物丰富度、多样性、产量和化学成分的影响。测量在 3 月至 6 月的最佳植被期进行,为期两年(2019 年和 2020 年)。实验设计包括一个因素:放牧强度(无放牧、适度放牧和高强度放牧)。结果表明,放牧降低了植物丰富度和多样性(最大多样性、香农和韦弗指数、均匀度指数),但对覆盖率没有影响。未放牧的休耕地植物种类更丰富,有 23 种草本植物,分布在 8 个植物科中,其中最多的是菊科(39%),其次是禾本科(26%)。放牧也显著降低了草本植物的生物量和地上植物总重量,而在非放牧条件下则有所改善,记录值分别为 3.80 吨 DM.ha-1 和 1.77 吨 DM.ha-1。此外,放牧对化学成分也有显著影响,中度放牧的数值最高,总氮含量达到 14.7%,矿物质含量达到 11.66%。
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引用次数: 0
Diversity and productive potential of timber trees in cocoa agroforestry systems in AltaVerapaz, Guatemala 危地马拉 AltaVerapaz 可可农林系统中用材树的多样性和生产潜力
Pub Date : 2024-02-27 DOI: 10.47280/revfacagron(luz).v41.n1.08
C. Villanueva-González, J. Ruiz-Chután, Z. Polesný, M. Kalousová, Cristóbal Villanueva, B. Lojka
Timber production in agroforestry systems can improve environmental and economic sustainability, especially for families living in rural areas. Despite its importance in Guatemala, little is known about the potential of wood and its contribution to the sustainability of traditional agroforestry systems. This study aims to analyze the timber resources in cocoa agroforestry systems (CAFS) of different ages, evaluating variables such as floristic composition, species diversity, and the potential to obtain timber from the species present in the evaluated CAFS. Twenty temporary sampling plots of 2,500 m2 were established, and dendrometric variables were measured in all plots. Species occurrence was statistically analyzed by cross-tabulation and Pearson's Chi-square test (χ2) to determine species' frequency distribution among the evaluated CAFS ages. Eight hundred twenty-seven trees of 38 species belonging to 19 families were identified. The most represented species in the age range of the evaluated CAFS were Gliricidia sepium (35.07 %), Swietenia macrophylla (19.11 %), and Inga sapindoides (7.62 %). The total shade trees occupied a basal area of 33.29 m2, and a total volume of 352.35 m3 was recorded, of which 148.9 m3 were for commercial use. The most frequent uses of the wood were firewood (38.21 %), thin plank (30.23 %), and poles (22.85 %), while the use of thick plank was the least common (8.71 %). The CAFS in the studied area are characterized by a wide diversity of shade tree species. These results provide a solid basis for promoting sustainable practices that foster the productivity and preservation of these systems, thus contributing to the sustainability and well-being of farmers.
农林系统中的木材生产可以改善环境和经济的可持续性,尤其是对生活在农村地区的家庭而言。尽管木材在危地马拉非常重要,但人们对木材的潜力及其对传统农林系统可持续性的贡献知之甚少。本研究旨在分析不同树龄的可可农林系统(CAFS)中的木材资源,评估可可农林系统中的植物组成、物种多样性以及从这些物种中获取木材的潜力等变量。共建立了 20 个面积为 2,500 平方米的临时取样地块,并对所有地块的树势变量进行了测量。通过交叉表和皮尔逊卡方检验(χ2)对物种出现情况进行统计分析,以确定物种在所评估的 CAFS 树龄中的频率分布。共鉴定了隶属于 19 个科 38 个物种的 827 棵树。在所评估的 CAFS 树龄范围内,最多的树种为 Gliricidia sepium(35.07%)、Swietenia macrophylla(19.11%)和 Inga sapindoides(7.62%)。所有遮荫树的基部面积为 33.29 平方米,总体积为 352.35 立方米,其中 148.9 立方米用于商业用途。最常用的木材是木柴(38.21%)、薄木板(30.23%)和木杆(22.85%),而使用厚木板的情况最少(8.71%)。研究地区的 CAFS 具有树荫树种多样性的特点。这些结果为推广可持续做法提供了坚实的基础,可促进这些系统的生产力和保护,从而为农民的可持续性和福祉做出贡献。
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引用次数: 0
Bioactive compounds and antioxidant activity of Annona muricata L. fruits located in Manabí, Ecuador 厄瓜多尔马纳比地区艳阳果的生物活性化合物和抗氧化活性
Pub Date : 2024-02-27 DOI: 10.47280/revfacagron.v41.n1.07
C. Párraga, Fernando Rincón-Acosta, Roy Barre-Zambrano, Jhon Vera-Cedeño, Plinio Vargas, Freddy Mendoza
El mesoendocarpio de Annona muricata L. (Annonaceae) presenta en su composición minerales esenciales, ácido ascórbico, fibra dietaría, polifenoles y acetogeninas, lo cual evidencia los beneficios nutracéuticos de este frutal. El objeto de este estudio fue determinar los compuestos bioactivos y la actividad antioxidante de frutos de A. muricata localizados en Manabí, Ecuador. Se determinó el contenido de vitamina C, fibra dietaría, minerales, polifenoles, flavonoides, actividad antioxidante y se identificó la presencia de acetogeninas. Se observó que los minerales potasio, calcio, sodio, zinc, magnesio y hierro son los componentes mayoritarios presentes en la ceniza de la pulpa de guanábana. Así mismo, se evidenció un contenido relevante de vitamina C (26,64 mg AA.100 g-1 pulpa), polifenoles totales (398,79 mg EAG.100 g-1 pulpa), flavonoides (192,20 mg EQ.100 g-1 pulpa) y alta actividad antioxidante (318,90 mmoles equivalentes de trolox.100 g-1 pulpa) en los frutos de A. muricata. Los extractos etanólicos obtenidos del fruto al aplicar la prueba de Kedde, presentaron un anillo de color rosado, lo cual es un indicador inequívoco de la presencia de acetogeninas, las cuales tienen efecto antineoplásico demostrado. El mesoendocarpio de A. muricata localizados en Manabí, Ecuador tiene un importante contenido de compuestos bioactivos: minerales esenciales (K, Ca, Zn, Mg), vitamina C, polifenoles, presencia de acetogeninas y una alta actividad antioxidante, propiedades que disminuyen el riesgo de padecer enfermedades degenerativas y el envejecimiento celular. Por lo tanto, se recomienda su consumo en la dieta diaria como fruta fresca y procesada, debido a que constituye una valiosa alternativa nutricional y terapéutica.
Annona muricata L.(芒果科)的中果皮含有人体必需的矿物质、抗坏血酸、膳食纤维、多酚和乙酰甙元,这证明了这种水果的营养保健功效。本研究旨在确定厄瓜多尔马纳比地区 A. muricata 果实的生物活性化合物和抗氧化活性。研究测定了维生素 C、膳食纤维、矿物质、多酚、类黄酮、抗氧化活性和乙酰甙元的含量。研究发现,酸果肉灰分中的主要成分是钾、钙、钠、锌、镁和铁等矿物质。同样,在 A. muricata 的果实中,维生素 C(26.64 毫克 AA.100 克-1 果肉)、总多酚(398.79 毫克 EAG.100 克-1 果肉)、类黄酮(192.20 毫克 EQ.100 克-1 果肉)的含量也很高,而且抗氧化活性也很高(318.90 毫摩尔当量的 trolox.100 克-1 果肉)。从果实中提取的乙醇萃取物在进行 Kedde 试验时显示出粉红色的环,这是乙酰苷元存在的明确指标,而乙酰苷元已被证实具有抗肿瘤作用。位于厄瓜多尔马纳比的 A. muricata 的中果皮含有大量生物活性化合物:必需矿物质(钾、钙、锌、镁)、维生素 C、多酚、乙酰甙元和高抗氧化活性,这些特性可降低退行性疾病和细胞老化的风险。因此,建议在日常饮食中食用新鲜水果和加工水果,因为它是一种有价值的营养和治疗替代品。
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引用次数: 0
Influence of inorganic fertilization on production parameters of two varieties of sweet potato 无机肥料对两个甘薯品种生产参数的影响
Pub Date : 2024-02-27 DOI: 10.47280/revfacagron(luz).v41.n1.09
Gloria Cobeña, Eddie Zambrano, Favio Ruilova, Xavier Ortiz, Chang Hwan
In order to determine the effect of inorganic fertilization treatments on the productive behavior of the sweetpotato varieties "INIAP-Toquecita" and "INIAP-Buena Vista", this research was carried out during the dry season (June–December 2022) at the Estación Experimental Portoviejo del Instituto Nacional de Investigaciones Agropecuarias-INIAP, in the Colón parish, canton Portoviejo, province of Manabí, Ecuador. A randomized complete block design was used with three replications, and the following variables were analyzed: percent yield, total number of guides per plant, length of guides, mass of 100 guides, foliage yield, and number and yield of commercial roots. The inorganic fertilization treatments under study were: 1. N+P2O5+K2O+S+Mg+B; 2. N+P2O5+K2O+S+Mg+B+Zn+Mn+Fe; 3. 50 % more N+P2O5+K2O+S+Mg+B with respect to treatment 1; 4. N+P2O5+K2O; and 5. no fertilizers (control). It was evidenced that the inorganic fertilization treatments implemented on the variety "INIAP-Buena Vista" outperformed the results of the control, while treatments 1 and 2 of inorganic fertilization implemented on the variety "INIAP-Toquecita" outperformed the control. It was observed that the most favorable scenario for maximizing the yield of commercial tuberous roots in the variety "INIAP-Buena Vista" was treatment 3, which had 50 % more macro and micronutrients per hectare added to the soil with respect to treatment 1. The varieties evaluated did not show nutritional deficiencies during the production cycle (120 days).
为了确定无机肥处理对甘薯品种 "INIAP-Toquecita "和 "INIAP-Buena Vista "产量的影响,本研究于旱季(2022年6月至12月)在厄瓜多尔马纳比省波托维耶霍县科隆教区的国家农业研究学院波托维耶霍实验基地(Estación Experimental Portoviejo del Instituto Nacional de Investigaciones Agropecuarias-INIAP)进行。采用随机完全区组设计,三次重复,分析了以下变量:产量百分比、每株导叶总数、导叶长度、100 根导叶的质量、叶片产量以及商品根的数量和产量。研究中的无机肥处理有1.N+P2O5+K2O+S+Mg+B;2.N+P2O5+K2O+S+Mg+B+Zn+Mn+Fe;3.N+P2O5+K2O+S+Mg+B 比处理 1 增加 50%;4.N+P2O5+K2O;5.不施肥(对照)。结果表明,在 "INIAP-Buena Vista "品种上施用无机肥的处理效果优于对照,而在 "INIAP-Toquecita "品种上施用无机肥的处理 1 和处理 2 的效果优于对照。据观察,对 "INIAP-Buena Vista "品种最大限度地提高商品块根产量最有利的方案是处理 3,与处理 1 相比,处理 3 每公顷土壤中添加的宏量和微量营养元素多 50%。所评估的品种在生产周期(120 天)内没有出现营养缺乏症。
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引用次数: 0
Typology of production units and livestock technologies for adaptation to drought in Sinaloa, Mexico 墨西哥锡那罗亚适应干旱的生产单位和畜牧技术类型
Pub Date : 2024-02-20 DOI: 10.47280/revfacagron(luz).v41.n1.06
V. Cuevas-Reyes, Alfredo Loaiza, Obed Gutiérrez, Germán Buendía, C. Rosales-Nieto
Drought as an effect of climate change affects the productivity and sustainability of livestock systems. The objective of this study was to analyze how technological land management for adaptation to climate change adopted by livestock farmers in southern Sinaloa, Mexico, corresponds to the typologies identified in the study area. A non-probabilistic sampling was applied, selecting 50 production units (UP) in six municipalities of Sinaloa, whose information was analyzed by cluster analysis and descriptive statistics. It were identified three livestock typologies. Cluster 1 (46 %), was defined as subsistence since its production units (PU) has few animals and showed the smallest total surface area, the producers are the oldest and use the shade in paddocks and the adjustment of stocking rates as drought mitigation practices. Cluster 2 (46 %), sowed the medium productive behavior, are younger producers whose PU showed the largest area of crops and rangeland, this group adopted stocking rate adjustment, forage conservation and species diversification as mitigation measures. Cluster 3 (8 %) showed the highest total area, livestock inventory and productivity levels; drought mitigation decisions are focused on stocking rate adjustment and forage conservation. The study identified mitigation practices related to land use from the farmers' point of view. These results can be used to conduct studies in similar environments and to scale adaptation measures for climate change from the local level and by type of farmer.
干旱是气候变化的一个影响因素,它影响着畜牧系统的生产力和可持续性。本研究的目的是分析墨西哥锡那罗亚州南部畜牧业者为适应气候变化而采用的土地管理技术如何与研究地区确定的类型相一致。研究采用了非概率抽样法,在锡那罗亚州的六个市镇选择了 50 个生产单位(UP),并通过聚类分析和描述性统计对其信息进行了分析。确定了三种牲畜类型。群组 1(46%)被定义为生计型,因为其生产单位(PU)的牲畜数量很少,总面积最小,生产者年龄最大,使用围场遮荫和调整放养率作为缓解干旱的措施。第 2 组(46%)为中等生产行为,生产者较年轻,其生产单位的农作物和牧场面积最大,该组采用调整放养率、保护牧草和物种多样化作为缓解措施。第 3 组(8%)的总面积、牲畜存栏数和生产率水平最高;旱情缓解决策的重点是调整放养率和保护饲草。这项研究从农民的角度确定了与土地利用有关的缓解措施。这些结果可用于在类似环境中开展研究,并从地方层面按农民类型推广气候变化适应措施。
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引用次数: 0
Influence of beneficial microorganisms on the agronomic behavior of potato crop cv.“Bicentenaria” 有益微生物对 "Bicentenaria "马铃薯作物农艺性状的影响
Pub Date : 2024-02-18 DOI: 10.47280/revfacagron(luz).v41.n1.05
Pedro Flores, Betsabe Leon
The excessive use of chemical fertilizers causes alterations in soil microbial activity, environmental pollution and high production costs in potato cultivation (Solanum tuberosum L.). One way to avoid this effect is with the use of beneficial microorganisms, due to their ability to capture atmospheric nitrogen, produce growth-promoting substances such as indole acetic acid and solubilize inorganic phosphorus from insoluble compounds, which in turn time improves crop yields. The objective of this research was to evaluate the effect of the beneficial microorganisms as biofertilizers on growth and yield of potato crop. In a population of 1,600 potato plants cv. “Bicentenaria” four treatments were evaluated: Trichoderma harzianum (0.5 g.L-1), Glomus spp. (30 g.plant-1), efficient microorganisms (EM) (50 mL.L-1) and a control treatment (no application of microorganisms). A completely randomized experimental design with four repetitions per treatment was used. The effect of the treatments was evaluated using vegetative and reproductive variables. It was evident that the treatment with T. harzianum significantly favored the rest of the treatments in plant height at 90 days (43.60 cm), tuber weight (154 g) and yield (57.13 t.ha-1). The use of Glomus spp. and EM, had only a partial effect on the growth of the plants. The treatment with T. harzianum could represent an ecological agricultural alternative for potato production.
在马铃薯种植(Solanum tuberosum L.)过程中,过量使用化肥会导致土壤微生物活性改变、环境污染和生产成本升高。避免这种影响的方法之一是使用有益微生物,因为它们能够捕捉大气中的氮,产生促进生长的物质(如吲哚乙酸),并从不溶性化合物中溶解无机磷,从而提高作物产量。这项研究的目的是评估有益微生物作为生物肥料对马铃薯作物生长和产量的影响。在种植了 1,600 株马铃薯的 "Bicentenaria "品种中,对四种处理方法进行了评估:毛霉(0.5 g.L-1)、菌胶团(30 g.plant-1)、高效微生物(EM)(50 mL.L-1)和对照处理(不施用微生物)。采用完全随机试验设计,每个处理重复四次。使用无性繁殖和繁殖变量评估了处理的效果。结果表明,在 90 天时的株高(43.60 厘米)、块茎重量(154 克)和产量(57.13 吨/公顷)方面,使用哈茨菌的处理明显优于其他处理。使用 Glomus spp.和 EM 只对植物生长产生部分影响。使用 T. harzianum 处理可作为马铃薯生产的一种生态农业替代方法。
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引用次数: 0
Molecular study of the “Escoba Blanca” variety of Sesamum indicum L. used in Paraguay 巴拉圭使用的 Sesamum indicum L.品种 "Escoba Blanca "的分子研究
Pub Date : 2024-02-09 DOI: 10.47280/revfacagron.v41.n1.04
Roberto Martínez-López, W. Pereira, A. Arrúa, Danilo Fernández, L. Centurión
The high quality of sesame seeds originating in the country has led Paraguay to be among the main exporters. Of the varieties available in Paraguayan territory, the most widespread is ‘‘Escoba Blanca’’, which, possibly due to the multiplication process, could promote changes in its allele frequency, diversity, and genetic purity. This work was carried out, aiming to determine the genetic differentiation between 50 populations/seedbeds/banks from seven Paraguayan companies collecting ‘‘Escoba Blanca’’ sesame, using microsatellite markers. These seven banks/companies/cooperatives collect and represent samples from all the producers/seedbeds located in different departments of the Eastern and Western Region (Chaco) of the country, with whom they work, market, and collect sesame. Plant tissue was obtained to extract DNA, from seedlings planted especially for the purpose, using all the included samples/accessions. Six microsatellite markers were used: GBssrsa184, GBssrsa123, GBssrsa182, GBssrsa108, GBssrsa08, and GBssrsa72. The following were calculated: number and frequency of alleles, distance/groupings, differentiation between populations, and their genetic structure. The mean number of alleles per locus ranged from 1.33 to 3.00. In the markers, GBssrsa184 and GBssrsa108, three populations presented a higher frequency of alleles. The populations examined exhibited a wide degree of genetic differentiation between them, with the identification of four groups, with greater and less purity respectively.Keywords: genetic diversity, genotype, microsatellite markers, Sesamum indicum L.
巴拉圭原产的芝麻质量上乘,因此成为主要出口国之一。在巴拉圭境内的芝麻品种中,分布最广的是 "Escoba Blanca",可能是由于繁殖过程,该品种的等位基因频率、多样性和遗传纯度都发生了变化。开展这项工作的目的是利用微卫星标记确定巴拉圭七家收集 "Escoba Blanca "芝麻的公司的 50 个种群/苗床/库之间的遗传差异。这七个库/公司/合作社收集并代表了来自该国东部和西部地区(查科)不同省份的所有生产者/种子基地的样本,他们与这些生产者/种子基地合作,销售并收集芝麻。从专门为此目的种植的秧苗中获取植物组织提取 DNA,使用的是所有包含的样本/品种。使用了六个微卫星标记:GBssrsa184、GBssrsa123、GBssrsa182、GBssrsa108、GBssrsa08 和 GBssrsa72。对等位基因的数量和频率、距离/分组、种群间的分化及其遗传结构进行了计算。每个位点的平均等位基因数从 1.33 到 3.00 不等。在标记 GBssrsa184 和 GBssrsa108 中,三个种群的等位基因频率较高。受检种群之间的遗传分化程度较高,分别鉴定出4个纯度较高和较低的群体。
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引用次数: 0
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Revista de la Facultad de Agronomía, Universidad del Zulia
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